RLG00000005371

phosphatase 2C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0005794
Physical Location & Seq
Forward (+)
67165578 .. 67168532
2955 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000005371

Sequence Viewer

Length: 999 bp
ATGAGTGAAGCTATCCAAAAATCGAAGCCAAAGCGCGAGAGCGATTCCGGGGATTCAAGCTCAGTTGCAAAGAAGCTGAAAATGGATCAAAGCCAAATCCCGGAAGTTGATTCGAGTGGAAGTGAGGCAAATTCAGATGCGAGGGAAATGGGTTTTGAAATCGAAGCTGAGGTAGCAGAGGATAAGGGGTTAAGGCACGCCATGGAGGACGCGTGGGTTCTGCTCTTGGATGCGAGCTTGGGTTTTCACGGAAACTTGAGGTGTGCACATTTTGCGATTTATGATGGGCATGGAGGTCGATTGGCTGCAGCGTATGCTCAGAAGCATTTGCATTCGAATGTGCTTTCGGCTGGATTACCACGTGAGTTGTTGAATGTGAAAGCTGCCAAGAAAGCTATTCTTGATGGTTTTCGGAAAACAGATGAGGCTATTCTTCAAGAAAGTGCTGCAGGGGGATGGCAAGACGGTGCTACAGCTGTATGTGTTTGGATATTAGGAAAAACAGTATTTGTTGCTAATATTGGGGATGCAAAAGCTGTGGTAGGAAGATCATCTCCTGTTGATAGATTGCAAAATGGTTCTGATGAAACACGTCTTTTGAAGGCCATTGTTTTAACTAGAGAACACAAAGCCATCTATGCACAGGAGCGTGCTCGTATTCAGAAGGCTGGTGGTAGTGTGAGTTCAAATGGACGACTACAGGGTCGCCTTGAAGTTTCTAGGGCCTTTGGAGATCGTCAGTTCAAAAAGTTTGGTGTTATTGCTACCCCAGATATTCATTCATTTGACCTTACAGAAAGAGAGCACTTCATAATTCTTGGTTGCGACGGCCTCTGGGGGGTAATTGGACCAAGTGATGCTGTTGATTTTGTTCAGAAACAATTAAAGGAGGGACTACCTGTTACAGCTATTAGCCGCTCTCTAGTTAGGGAAGCTGTGCGTGAGCGGCGCTGTAAAGATAACTGCACGGCAATCATAATCCTTTTCAGGCACAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

333

Amino Acids

36.2

Weight (kDa)

8.51

Isoelectric Point (pI)

46.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 60 - 320 1.4e-54 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 702
AccBSI CCGCTC 2 cut(s) 918, 946
AccII CGCG 2 cut(s) 36, 212
AciI CCGC 2 cut(s) 916, 946
AclWI GGATC 1 cut(s) 93
AcsI RAATTY 1 cut(s) 130
AcvI CACGTG 1 cut(s) 362
AfiI CCNNNNNNNGG 2 cut(s) 100, 838
AflIII ACRYGT 2 cut(s) 210, 590
AgsI TTSAA 8 cut(s) 57, 158, 373, 437, 601, 687, 713, 745
AjiI CACGTC 1 cut(s) 593
Alw21I GWGCWC 3 cut(s) 268, 655, 807
Alw44I GTGCAC 1 cut(s) 264
AlwI GGATC 1 cut(s) 93
AoxI GGCC 3 cut(s) 603, 723, 829
ApaLI GTGCAC 1 cut(s) 264
ApeKI GCWGC 4 cut(s) 305, 308, 383, 446
ApoI RAATTY 1 cut(s) 130
AspLEI GCGC 2 cut(s) 36, 951
AspS9I GGNCC 2 cut(s) 723, 848
AsuC2I CCSGG 2 cut(s) 49, 101
AsuII TTCGAA 1 cut(s) 335
AvaII GGWCC 1 cut(s) 848
BaeGI GKGCMC 1 cut(s) 268
BbrPI CACGTG 1 cut(s) 362
Bbv12I GWGCWC 3 cut(s) 268, 655, 807
BbvCI CCTCAGC 1 cut(s) 168
BbvI GCAGC 4 cut(s) 292, 320, 370, 433
BccI CCATC 4 cut(s) 278, 398, 450, 641
BceAI ACGGC 2 cut(s) 844, 984
BcgI CGANNNNNNTGC 2 cut(s) 278, 312
BcnI CCSGG 2 cut(s) 49, 101
BfaI CTAG 3 cut(s) 618, 720, 923
BfmI CTRYAG 4 cut(s) 306, 447, 471, 698
BfoI RGCGCY 1 cut(s) 952
BisI GCNGC 6 cut(s) 306, 309, 384, 447, 916, 947
BlsI GCNGC 6 cut(s) 307, 310, 385, 448, 917, 948
Bme1390I CCNGG 2 cut(s) 49, 101
Bme18I GGWCC 1 cut(s) 848
BmgBI CACGTC 1 cut(s) 593
BmgT120I GGNCC 2 cut(s) 723, 848
BmrFI CCNGG 2 cut(s) 49, 101
BmsI GCATC 4 cut(s) 127, 220, 517, 847
Bpu10I CCTNAGC 1 cut(s) 168
Bpu14I TTCGAA 1 cut(s) 335
BpuEI CTTGAG 1 cut(s) 277
BpuMI CCSGG 2 cut(s) 49, 101
BsaAI YACGTR 1 cut(s) 362
BsaJI CCNNGG 2 cut(s) 48, 201
BsaXI ACNNNNNCTCC 2 cut(s) 197, 227
Bsc4I CCNNNNNNNGG 2 cut(s) 100, 838
BseDI CCNNGG 2 cut(s) 48, 201
BseGI GGATG 3 cut(s) 235, 461, 532
BseLI CCNNNNNNNGG 2 cut(s) 100, 838
BseMII CTCAG 3 cut(s) 75, 159, 332
BseSI GKGCMC 1 cut(s) 268
BseXI GCAGC 4 cut(s) 292, 320, 370, 433
BsgI GTGCAG 1 cut(s) 949
Bsh1236I CGCG 2 cut(s) 36, 212
BshFI GGCC 3 cut(s) 605, 725, 831
BsiHKAI GWGCWC 3 cut(s) 268, 655, 807
BsiSI CCGG 2 cut(s) 48, 101
BslFI GGGAC 1 cut(s) 906
BslI CCNNNNNNNGG 2 cut(s) 100, 838
BsmFI GGGAC 1 cut(s) 906
BsmI GAATGC 1 cut(s) 331
BsnI GGCC 3 cut(s) 605, 725, 831
Bsp119I TTCGAA 1 cut(s) 335
Bsp1286I GDGCHC 3 cut(s) 268, 655, 807
Bsp143I GATC 3 cut(s) 85, 548, 733
Bsp19I CCATGG 1 cut(s) 201
BspACI CCGC 2 cut(s) 916, 946
BspANI GGCC 3 cut(s) 605, 725, 831
BspCNI CTCAG 3 cut(s) 74, 160, 331
BspFNI CGCG 2 cut(s) 36, 212
BspMAI CTGCAG 2 cut(s) 310, 451
BspPI GGATC 1 cut(s) 93
BspT104I TTCGAA 1 cut(s) 335
BsrBI CCGCTC 2 cut(s) 918, 946
BssECI CCNNGG 2 cut(s) 48, 201
BssMI GATC 3 cut(s) 85, 548, 733
BssT1I CCWWGG 1 cut(s) 201
Bst4CI ACNGT 2 cut(s) 467, 505
BstAPI GCANNNNNTGC 2 cut(s) 272, 314
BstBAI YACGTR 1 cut(s) 362
BstBI TTCGAA 1 cut(s) 335
BstC8I GCNNGC 3 cut(s) 198, 235, 651
BstDEI CTNAG 3 cut(s) 61, 168, 318
BstDSI CCRYGG 1 cut(s) 201
BstF5I GGATG 3 cut(s) 235, 461, 532
BstFNI CGCG 2 cut(s) 36, 212
BstH2I RGCGCY 1 cut(s) 952
BstHHI GCGC 2 cut(s) 36, 951
BstKTI GATC 3 cut(s) 88, 551, 736
BstMBI GATC 3 cut(s) 85, 548, 733
BstMWI GCNNNNNNNGC 6 cut(s) 173, 272, 314, 392, 638, 946
BstSCI CCNGG 2 cut(s) 47, 99
BstSFI CTRYAG 4 cut(s) 306, 447, 471, 698
BstSLI GKGCMC 1 cut(s) 268
BstUI CGCG 2 cut(s) 36, 212
BstV1I GCAGC 4 cut(s) 292, 320, 370, 433
BsuRI GGCC 3 cut(s) 605, 725, 831
BtgI CCRYGG 1 cut(s) 201
BtrI CACGTC 1 cut(s) 593
BtsCI GGATG 3 cut(s) 235, 461, 532
Cac8I GCNNGC 3 cut(s) 198, 235, 651
CfoI GCGC 2 cut(s) 36, 951
Cfr13I GGNCC 2 cut(s) 723, 848
CseI GACGC 1 cut(s) 218
CspCI CAANNNNNGTGG 4 cut(s) 348, 383, 519, 554
CviAII CATG 2 cut(s) 202, 290
DdeI CTNAG 3 cut(s) 61, 168, 318
DpnI GATC 3 cut(s) 87, 550, 735
DpnII GATC 3 cut(s) 85, 548, 733
DrdI GACNNNNNNGTC 1 cut(s) 702
DseDI GACNNNNNNGTC 1 cut(s) 702
Eco130I CCWWGG 1 cut(s) 201
Eco47I GGWCC 1 cut(s) 848
Eco72I CACGTG 1 cut(s) 362
EcoO109I RGGNCCY 1 cut(s) 723
EcoT14I CCWWGG 1 cut(s) 201
ErhI CCWWGG 1 cut(s) 201
FaeI CATG 2 cut(s) 205, 293
FaiI YATR 8 cut(s) 203, 282, 291, 315, 481, 639, 812, 977
FalI AAGNNNNNCTT 2 cut(s) 384, 416
FaqI GGGAC 1 cut(s) 906
FatI CATG 2 cut(s) 201, 289
Fnu4HI GCNGC 6 cut(s) 306, 309, 384, 447, 916, 947
FokI GGATG 3 cut(s) 242, 468, 539
Fsp4HI GCNGC 6 cut(s) 306, 309, 384, 447, 916, 947
FspBI CTAG 3 cut(s) 618, 720, 923
GlaI GCGC 2 cut(s) 35, 950
GluI GCNGC 6 cut(s) 306, 309, 384, 447, 916, 947
HaeII RGCGCY 1 cut(s) 952
HaeIII GGCC 3 cut(s) 605, 725, 831
HapII CCGG 2 cut(s) 48, 101
HgaI GACGC 1 cut(s) 218
HhaI GCGC 2 cut(s) 36, 951
Hin1II CATG 2 cut(s) 205, 293
Hin6I GCGC 2 cut(s) 34, 949
HinP1I GCGC 2 cut(s) 34, 949
HinfI GANTC 3 cut(s) 44, 53, 110
HpaII CCGG 2 cut(s) 48, 101
Hpy166II GTNNAC 1 cut(s) 266
Hpy188I TCNGA 6 cut(s) 136, 321, 414, 583, 663, 876
Hpy188III TCNNGA 2 cut(s) 401, 437
Hpy8I GTNNAC 1 cut(s) 266
Hpy99I CGWCG 1 cut(s) 830
HpyAV CCTTC 2 cut(s) 595, 658
HpyCH4III ACNGT 2 cut(s) 467, 505
HpyCH4IV ACGT 2 cut(s) 361, 592
HpyCH4V TGCA 9 cut(s) 68, 266, 308, 331, 449, 530, 571, 641, 966
HpyF10VI GCNNNNNNNGC 6 cut(s) 173, 272, 314, 392, 638, 946
HpyF3I CTNAG 3 cut(s) 61, 168, 318
HpySE526I ACGT 2 cut(s) 361, 592
Hsp92II CATG 2 cut(s) 205, 293
HspAI GCGC 2 cut(s) 34, 949
Kzo9I GATC 3 cut(s) 85, 548, 733
LmnI GCTCC 1 cut(s) 646
Lsp1109I GCAGC 4 cut(s) 292, 320, 370, 433
LweI GCATC 4 cut(s) 127, 220, 517, 847
MaeI CTAG 3 cut(s) 618, 720, 923
MaeII ACGT 2 cut(s) 361, 592
MaeIII GTNAC 1 cut(s) 901
MalI GATC 3 cut(s) 87, 550, 735
MbiI CCGCTC 2 cut(s) 918, 946
MboI GATC 3 cut(s) 85, 548, 733
MboII GAAGA 2 cut(s) 425, 558
MhlI GDGCHC 3 cut(s) 268, 655, 807
MluCI AATT 4 cut(s) 130, 813, 843, 881
MluI ACGCGT 1 cut(s) 210
MseI TTAA 3 cut(s) 191, 614, 884
MslI CAYNNNNRTG 1 cut(s) 336
MspA1I CMGCKG 1 cut(s) 476
MspI CCGG 2 cut(s) 48, 101
MspR9I CCNGG 2 cut(s) 49, 101
Mva1269I GAATGC 1 cut(s) 331
MvnI CGCG 2 cut(s) 36, 212
MwoI GCNNNNNNNGC 6 cut(s) 173, 272, 314, 392, 638, 946
NciI CCSGG 2 cut(s) 49, 101
NcoI CCATGG 1 cut(s) 201
NdeII GATC 3 cut(s) 85, 548, 733
NlaIII CATG 2 cut(s) 205, 293
NspV TTCGAA 1 cut(s) 335
PctI GAATGC 1 cut(s) 331
PfeI GAWTC 3 cut(s) 44, 53, 110
PfoI TCCNGGA 1 cut(s) 99
PkrI GCNGC 6 cut(s) 307, 310, 385, 448, 917, 948
PmaCI CACGTG 1 cut(s) 362
PmlI CACGTG 1 cut(s) 362
Ppu21I YACGTR 1 cut(s) 362
PspCI CACGTG 1 cut(s) 362
PspPI GGNCC 2 cut(s) 723, 848
PsrI GAACNNNNNNTAC 2 cut(s) 667, 699
PstI CTGCAG 2 cut(s) 310, 451
PvuII CAGCTG 1 cut(s) 476
RseI CAYNNNNRTG 1 cut(s) 336
SaqAI TTAA 3 cut(s) 191, 614, 884
SatI GCNGC 6 cut(s) 306, 309, 384, 447, 916, 947
Sau3AI GATC 3 cut(s) 85, 548, 733
Sau96I GGNCC 2 cut(s) 723, 848
ScrFI CCNGG 2 cut(s) 49, 101
SduI GDGCHC 3 cut(s) 268, 655, 807
SfaNI GCATC 4 cut(s) 127, 220, 517, 847
SfcI CTRYAG 4 cut(s) 306, 447, 471, 698
SfuI TTCGAA 1 cut(s) 335
SinI GGWCC 1 cut(s) 848
SmiMI CAYNNNNRTG 1 cut(s) 336
SmlI CTYRAG 1 cut(s) 256
SmoI CTYRAG 1 cut(s) 256
Sse9I AATT 4 cut(s) 130, 813, 843, 881
SsiI CCGC 2 cut(s) 916, 946
SspI AATATT 1 cut(s) 520
SspMI CTAG 3 cut(s) 618, 720, 923
StyD4I CCNGG 2 cut(s) 47, 99
StyI CCWWGG 1 cut(s) 201
TaaI ACNGT 2 cut(s) 467, 505
TaiI ACGT 2 cut(s) 364, 595
TaqI TCGA 5 cut(s) 23, 113, 162, 298, 335
TasI AATT 4 cut(s) 130, 813, 843, 881
TauI GCSGC 2 cut(s) 918, 949
TfiI GAWTC 3 cut(s) 44, 53, 110
Tru1I TTAA 3 cut(s) 191, 614, 884
Tru9I TTAA 3 cut(s) 191, 614, 884
TseI GCWGC 4 cut(s) 305, 308, 383, 446
TspDTI ATGAA 4 cut(s) 600, 767, 771, 799
TspGWI ACGGA 1 cut(s) 264
VneI GTGCAC 1 cut(s) 264
VpaK11BI GGWCC 1 cut(s) 848
XapI RAATTY 1 cut(s) 130
XspI CTAG 3 cut(s) 618, 720, 923
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.