Rorug06G0422600

phosphatase 2C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0005794
Physical Location & Seq
Forward (+)
56087955 .. 56090377
2423 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0422600.1

Sequence Viewer

Length: 1023 bp
ATGGCTGGGTTCTTCGGTAATTTAGGGGGAAGAGATCAAGGGCCGAGAAACAAACAAGACGGAGACGATGAAAGAGGAGGAAATTTAAACCTGTTTTCGTACGGAAACGAAGAGATCTACAACAACAAGGGGGGTTTCGAGTTATGGCCACAGTATCATCCTCACCATCCTCATCATCAGCAAAGCTTGAACTACTACGCGTTTGGAGTGGATCCTAGCCGTAATAACAACCACAACAACTTGGAGGGCAACGTCTCGGATGACTCATCTTCGGGGTTGAGACTCACTGTGATGCGACAAGGAAGCGGCGGTGGCGGTGGCGGTGGCGGTAGTGGTCTAGGATCGGGCGGAATGAATTGTCAGGATTGCGGGAATCAAGCTAAGAAAGACTGTCCTCACTTGCGGTGCCGAACCTGTTGCAAGAGCCGAGGGTTTCAGTGCCAAACACACGTCAAGAGCACTTGGGTTCCTGCCGCGAAACGCCGCGAGAGGCAGGAACAATTCTCTGCTCTTCAGCAACAACAACAACAACAGCAGTTACAGTTTCCGAAAAGGCAGAGAGACAATCAAGCAGGAGGAGCTGGAGGCTCTTCTCTAGCCTGCGTTCGTATACCCACTAACACGTCAGGGTTGGAGCTTGCACCATTTCCAGCGGAGGTGAGCTCGCCAGCCCTGTTCCGGTGTGTGAAAGTCAGTGCAATGGACGATGTGGAGGAGCAGTACGCGTACCAGACGGCGGTGAACATCGGAGGGCACGTGTTCAAGGGACTTCTCTACGATCAAGGACTGGATGGTCATTACACAAGCGCCACCACCGCCGGCGATAGCTCCTCAGGCCAACAAGACGGAGGAGGCGGAGGGGGACATCATCATAATAATCAACAGCAACTTAATCTTATAACCACCACAAGCACAAGTGCCAACCCACCTACGGCGACGTTGCTCGACCCTACGGTGGTGTTTCCGACTCCGCTGAATGCTTTTGTGGCTGGTACGCAATACTTCCCACCTCCGAGGTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

340

Amino Acids

36.48

Weight (kDa)

6.88

Isoelectric Point (pI)

57.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF702 PF05142 117 - 264 1.7e-63 Domain of unknown function (DUF702)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 899
AasI GACNNNNNNGTC 1 cut(s) 792
AccB1I GGYRCC 1 cut(s) 405
AccI GTMKAC 1 cut(s) 610
AccII CGCG 4 cut(s) 200, 476, 486, 725
AclWI GGATC 3 cut(s) 206, 219, 349
AcoI YGGCCR 1 cut(s) 146
AcsI RAATTY 1 cut(s) 82
AcuI CTGAAG 1 cut(s) 497
AcvI CACGTG 1 cut(s) 757
AfaI GTAC 4 cut(s) 101, 722, 728, 994
AfiI CCNNNNNNNGG 4 cut(s) 678, 736, 931, 955
AflIII ACRYGT 5 cut(s) 198, 448, 621, 723, 756
AgsI TTSAA 2 cut(s) 190, 763
AjiI CACGTC 2 cut(s) 451, 624
AluBI AGCT 6 cut(s) 186, 380, 581, 637, 663, 828
AluI AGCT 6 cut(s) 186, 380, 581, 637, 663, 828
Alw21I GWGCWC 2 cut(s) 461, 665
Alw26I GTCTC 4 cut(s) 57, 259, 274, 555
AlwI GGATC 3 cut(s) 206, 219, 349
AoxI GGCC 3 cut(s) 41, 146, 835
ApoI RAATTY 1 cut(s) 82
AspLEI GCGC 1 cut(s) 809
AspS9I GGNCC 1 cut(s) 41
AsuHPI GGTGA 3 cut(s) 155, 670, 751
AxyI CCTNAGG 1 cut(s) 832
BaeGI GKGCMC 1 cut(s) 756
BalI TGGCCA 1 cut(s) 148
BamHI GGATCC 1 cut(s) 211
BanI GGYRCC 1 cut(s) 405
BanII GRGCYC 1 cut(s) 665
BbrPI CACGTG 1 cut(s) 757
Bbv12I GWGCWC 2 cut(s) 461, 665
BccI CCATC 2 cut(s) 174, 785
BceAI ACGGC 3 cut(s) 204, 750, 948
BcgI CGANNNNNNTGC 2 cut(s) 399, 433
BcoDI GTCTC 4 cut(s) 57, 259, 274, 555
BfaI CTAG 3 cut(s) 216, 338, 596
BfoI RGCGCY 1 cut(s) 810
BglII AGATCT 1 cut(s) 114
BisI GCNGC 3 cut(s) 307, 474, 484
BlsI GCNGC 3 cut(s) 308, 475, 485
BmgBI CACGTC 2 cut(s) 451, 624
BmgT120I GGNCC 1 cut(s) 41
BmiI GGNNCC 3 cut(s) 213, 407, 468
BmsI GCATC 1 cut(s) 282
BplI GAGNNNNNCTC 2 cut(s) 647, 679
BpmI CTGGAG 1 cut(s) 603
BsaAI YACGTR 1 cut(s) 757
BsaJI CCNNGG 2 cut(s) 427, 1013
BsaWI WCCGGW 1 cut(s) 678
BsaXI ACNNNNNCTCC 4 cut(s) 704, 734, 741, 771
Bsc4I CCNNNNNNNGG 4 cut(s) 678, 736, 931, 955
Bse118I RCCGGY 1 cut(s) 818
Bse1I ACTGG 1 cut(s) 792
Bse21I CCTNAGG 1 cut(s) 832
Bse3DI GCAATG 1 cut(s) 705
BseDI CCNNGG 2 cut(s) 427, 1013
BseGI GGATG 4 cut(s) 157, 166, 265, 796
BseLI CCNNNNNNNGG 4 cut(s) 678, 736, 931, 955
BseMI GCAATG 1 cut(s) 705
BseMII CTCAG 1 cut(s) 846
BseNI ACTGG 1 cut(s) 792
BseRI GAGGAG 5 cut(s) 90, 591, 728, 820, 864
BseSI GKGCMC 1 cut(s) 756
BseYI CCCAGC 1 cut(s) 5
Bsh1236I CGCG 4 cut(s) 200, 476, 486, 725
BshFI GGCC 3 cut(s) 43, 148, 837
BshNI GGYRCC 1 cut(s) 405
BsiHKAI GWGCWC 2 cut(s) 461, 665
BsiSI CCGG 2 cut(s) 679, 819
BsiWI CGTACG 1 cut(s) 99
BslFI GGGAC 2 cut(s) 780, 876
BslI CCNNNNNNNGG 4 cut(s) 678, 736, 931, 955
BsmAI GTCTC 4 cut(s) 57, 259, 274, 555
BsmBI CGTCTC 2 cut(s) 57, 259
BsmFI GGGAC 2 cut(s) 780, 876
BsmI GAATGC 1 cut(s) 982
BsnI GGCC 3 cut(s) 43, 148, 837
Bsp1286I GDGCHC 3 cut(s) 461, 665, 756
Bsp143I GATC 5 cut(s) 34, 114, 211, 341, 778
BspANI GGCC 3 cut(s) 43, 148, 837
BspCNI CTCAG 1 cut(s) 845
BspFNI CGCG 4 cut(s) 200, 476, 486, 725
BspLI GGNNCC 3 cut(s) 213, 407, 468
BspPI GGATC 3 cut(s) 206, 219, 349
BspQI GCTCTTC 2 cut(s) 516, 595
BspT107I GGYRCC 1 cut(s) 405
BsrDI GCAATG 1 cut(s) 705
BsrFI RCCGGY 1 cut(s) 818
BsrI ACTGG 1 cut(s) 792
BssAI RCCGGY 1 cut(s) 818
BssECI CCNNGG 2 cut(s) 427, 1013
BssMI GATC 5 cut(s) 34, 114, 211, 341, 778
BssNAI GTATAC 1 cut(s) 611
Bst1107I GTATAC 1 cut(s) 611
Bst4CI ACNGT 5 cut(s) 153, 289, 392, 543, 955
Bst6I CTCTTC 4 cut(s) 25, 105, 516, 595
BstBAI YACGTR 1 cut(s) 757
BstC8I GCNNGC 5 cut(s) 601, 639, 665, 669, 820
BstDEI CTNAG 3 cut(s) 381, 832, 1020
BstF5I GGATG 4 cut(s) 157, 166, 265, 796
BstFNI CGCG 4 cut(s) 200, 476, 486, 725
BstH2I RGCGCY 1 cut(s) 810
BstHHI GCGC 1 cut(s) 809
BstKTI GATC 5 cut(s) 37, 117, 214, 344, 781
BstMAI GTCTC 4 cut(s) 57, 259, 274, 555
BstMBI GATC 5 cut(s) 34, 114, 211, 341, 778
BstMWI GCNNNNNNNGC 5 cut(s) 312, 578, 815, 834, 986
BstSLI GKGCMC 1 cut(s) 756
BstUI CGCG 4 cut(s) 200, 476, 486, 725
BstX2I RGATCY 2 cut(s) 114, 211
BstYI RGATCY 2 cut(s) 114, 211
BstZ17I GTATAC 1 cut(s) 611
Bsu36I CCTNAGG 1 cut(s) 832
BsuRI GGCC 3 cut(s) 43, 148, 837
BtrI CACGTC 2 cut(s) 451, 624
BtsCI GGATG 4 cut(s) 157, 166, 265, 796
BtsIMutI CAGTG 3 cut(s) 285, 443, 700
Cac8I GCNNGC 5 cut(s) 601, 639, 665, 669, 820
CfoI GCGC 1 cut(s) 809
Cfr10I RCCGGY 1 cut(s) 818
Cfr13I GGNCC 1 cut(s) 41
Csp6I GTAC 4 cut(s) 100, 721, 727, 993
CviQI GTAC 4 cut(s) 100, 721, 727, 993
DdeI CTNAG 3 cut(s) 381, 832, 1020
DpnI GATC 5 cut(s) 36, 116, 213, 343, 780
DpnII GATC 5 cut(s) 34, 114, 211, 341, 778
DraI TTTAAA 1 cut(s) 87
DrdI GACNNNNNNGTC 1 cut(s) 792
DseDI GACNNNNNNGTC 1 cut(s) 792
EaeI YGGCCR 1 cut(s) 146
Eam1104I CTCTTC 4 cut(s) 25, 105, 516, 595
EarI CTCTTC 4 cut(s) 25, 105, 516, 595
EciI GGCGGA 2 cut(s) 363, 870
Ecl136II GAGCTC 1 cut(s) 663
Eco24I GRGCYC 1 cut(s) 665
Eco53kI GAGCTC 1 cut(s) 663
Eco57I CTGAAG 1 cut(s) 497
Eco72I CACGTG 1 cut(s) 757
Eco81I CCTNAGG 1 cut(s) 832
EcoICRI GAGCTC 1 cut(s) 663
EcoT38I GRGCYC 1 cut(s) 665
Esp3I CGTCTC 2 cut(s) 57, 259
FaiI YATR 4 cut(s) 145, 611, 873, 899
FaqI GGGAC 2 cut(s) 780, 876
FauI CCCGC 1 cut(s) 362
FblI GTMKAC 1 cut(s) 610
Fnu4HI GCNGC 3 cut(s) 307, 474, 484
FokI GGATG 4 cut(s) 144, 153, 272, 803
FriOI GRGCYC 1 cut(s) 665
Fsp4HI GCNGC 3 cut(s) 307, 474, 484
FspBI CTAG 3 cut(s) 216, 338, 596
GlaI GCGC 1 cut(s) 808
GluI GCNGC 3 cut(s) 307, 474, 484
GsaI CCCAGC 1 cut(s) 9
GsuI CTGGAG 1 cut(s) 603
HaeII RGCGCY 1 cut(s) 810
HaeIII GGCC 3 cut(s) 43, 148, 837
HapII CCGG 2 cut(s) 679, 819
HhaI GCGC 1 cut(s) 809
Hin6I GCGC 1 cut(s) 807
HinP1I GCGC 1 cut(s) 807
HindIII AAGCTT 1 cut(s) 184
HinfI GANTC 4 cut(s) 263, 282, 373, 967
HpaII CCGG 2 cut(s) 679, 819
HphI GGTGA 3 cut(s) 155, 670, 751
Hpy166II GTNNAC 2 cut(s) 611, 742
Hpy188I TCNGA 5 cut(s) 259, 549, 749, 966, 1014
Hpy188III TCNNGA 2 cut(s) 362, 454
Hpy8I GTNNAC 2 cut(s) 611, 742
Hpy99I CGWCG 1 cut(s) 940
HpyCH4III ACNGT 5 cut(s) 153, 289, 392, 543, 955
HpyCH4IV ACGT 5 cut(s) 252, 450, 623, 756, 938
HpyCH4V TGCA 3 cut(s) 420, 641, 698
HpyF10VI GCNNNNNNNGC 5 cut(s) 312, 578, 815, 834, 986
HpyF3I CTNAG 3 cut(s) 381, 832, 1020
HpySE526I ACGT 5 cut(s) 252, 450, 623, 756, 938
HspAI GCGC 1 cut(s) 807
KroI GCCGGC 1 cut(s) 818
KroNI GCCGGC 1 cut(s) 820
Kzo9I GATC 5 cut(s) 34, 114, 211, 341, 778
LguI GCTCTTC 2 cut(s) 516, 595
LmnI GCTCC 4 cut(s) 578, 634, 715, 833
LweI GCATC 1 cut(s) 282
MaeI CTAG 3 cut(s) 216, 338, 596
MaeII ACGT 5 cut(s) 252, 450, 623, 756, 938
MaeIII GTNAC 1 cut(s) 537
MalI GATC 5 cut(s) 36, 116, 213, 343, 780
MboI GATC 5 cut(s) 34, 114, 211, 341, 778
MboII GAAGA 6 cut(s) 4, 42, 122, 261, 503, 582
MflI RGATCY 2 cut(s) 114, 211
MhlI GDGCHC 3 cut(s) 461, 665, 756
MlsI TGGCCA 1 cut(s) 148
MluCI AATT 4 cut(s) 19, 82, 355, 500
MluI ACGCGT 2 cut(s) 198, 723
MluNI TGGCCA 1 cut(s) 148
MlyI GAGTC 3 cut(s) 257, 276, 961
MmeI TCCRAC 2 cut(s) 612, 989
Mox20I TGGCCA 1 cut(s) 148
MreI CGCCGGCG 1 cut(s) 818
MroNI GCCGGC 1 cut(s) 818
MscI TGGCCA 1 cut(s) 148
MseI TTAA 2 cut(s) 86, 891
MslI CAYNNNNRTG 1 cut(s) 290
Msp20I TGGCCA 1 cut(s) 148
MspA1I CMGCKG 2 cut(s) 653, 973
MspI CCGG 2 cut(s) 679, 819
Mva1269I GAATGC 1 cut(s) 982
MvnI CGCG 4 cut(s) 200, 476, 486, 725
MwoI GCNNNNNNNGC 5 cut(s) 312, 578, 815, 834, 986
NaeI GCCGGC 1 cut(s) 820
NdeII GATC 5 cut(s) 34, 114, 211, 341, 778
NgoMIV GCCGGC 1 cut(s) 818
NlaIV GGNNCC 3 cut(s) 213, 407, 468
NmeAIII GCCGAG 2 cut(s) 69, 452
PciSI GCTCTTC 2 cut(s) 516, 595
PcsI WCGNNNNNNNCGW 1 cut(s) 753
PctI GAATGC 1 cut(s) 982
PdiI GCCGGC 1 cut(s) 820
PfeI GAWTC 1 cut(s) 373
Pfl23II CGTACG 1 cut(s) 99
PkrI GCNGC 3 cut(s) 308, 475, 485
PleI GAGTC 3 cut(s) 257, 276, 961
PmaCI CACGTG 1 cut(s) 757
PmlI CACGTG 1 cut(s) 757
PpsI GAGTC 3 cut(s) 257, 276, 961
Ppu21I YACGTR 1 cut(s) 757
PsiI TTATAA 1 cut(s) 899
Psp124BI GAGCTC 1 cut(s) 665
PspCI CACGTG 1 cut(s) 757
PspFI CCCAGC 1 cut(s) 5
PspLI CGTACG 1 cut(s) 99
PspN4I GGNNCC 3 cut(s) 213, 407, 468
PspPI GGNCC 1 cut(s) 41
PsuI RGATCY 2 cut(s) 114, 211
RsaI GTAC 4 cut(s) 101, 722, 728, 994
RsaNI GTAC 4 cut(s) 100, 721, 727, 993
RseI CAYNNNNRTG 1 cut(s) 290
SacI GAGCTC 1 cut(s) 665
SapI GCTCTTC 2 cut(s) 516, 595
SaqAI TTAA 2 cut(s) 86, 891
SatI GCNGC 3 cut(s) 307, 474, 484
Sau3AI GATC 5 cut(s) 34, 114, 211, 341, 778
Sau96I GGNCC 1 cut(s) 41
SchI GAGTC 3 cut(s) 257, 276, 961
SduI GDGCHC 3 cut(s) 461, 665, 756
SfaNI GCATC 1 cut(s) 282
SgrAI CRCCGGYG 1 cut(s) 818
SmiMI CAYNNNNRTG 1 cut(s) 290
Sse9I AATT 4 cut(s) 19, 82, 355, 500
SspMI CTAG 3 cut(s) 216, 338, 596
SstI GAGCTC 1 cut(s) 665
TaaI ACNGT 5 cut(s) 153, 289, 392, 543, 955
TaiI ACGT 5 cut(s) 255, 453, 626, 759, 941
TaqI TCGA 2 cut(s) 138, 945
TasI AATT 4 cut(s) 19, 82, 355, 500
TauI GCSGC 3 cut(s) 309, 476, 486
TfiI GAWTC 1 cut(s) 373
Tru1I TTAA 2 cut(s) 86, 891
Tru9I TTAA 2 cut(s) 86, 891
TscAI CASTG 3 cut(s) 292, 443, 700
TspDTI ATGAA 2 cut(s) 84, 368
TspGWI ACGGA 3 cut(s) 75, 117, 861
TspRI CASTG 3 cut(s) 292, 443, 700
XapI RAATTY 1 cut(s) 82
XmiI GTMKAC 1 cut(s) 610
XspI CTAG 3 cut(s) 216, 338, 596
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.