Rorug04G0053600

phosphatase 2C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0005794
Physical Location & Seq
Forward (+)
8326061 .. 8328788
2728 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0053600.1

Sequence Viewer

Length: 531 bp
ATGGAAGTGGAACGGGCCAACTCGTCCCAATACCCGAAGCCAATAAGTAGTCAAGCACCTAGTTACTCGCTACTGACGACTCTGATTTTTCAGACTAAAACCTCTTCCCACACAGCACAGTCTGCAACTACTCGATTGAAAATGGCAGACGAAGCCAACAGAGCTGCGTTCATGGAGATTCAAGGTCGCATGATCGAGCTCACCGCCAAACTCAAGCAGGTGCAGAACCAGATGCGAAACAAAGAAGGAGAAAAGAAGCGTGCTTTTCTAACCCTAGAGGAAATTCGCCCTTTGCCTGATGACGCCAATACTTACAAATCCATAGGAAGAACGTTTGTTTTAGAGCCCAAGTCAGTGTTGGTGAATGAACAGGAGCAGAAGCTCAAGGATAGTGAGAGCGCAATTGCCTCACTACAGACCTCAAAGGAATACATTGAGAAACAGATTGGAGAGGTGGAGAGCAACTTGAGGGAGCTATTGAACCAGGATCCAAGTCTTGCTCGTCAGATAATGTCCATGACTGTAATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

176

Amino Acids

19.94

Weight (kDa)

7.88

Isoelectric Point (pI)

51.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Prefoldin_2 PF01920 59 - 162 3.5e-21 Prefoldin subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 208
Acc36I ACCTGC 1 cut(s) 208
AciI CCGC 1 cut(s) 204
AclI AACGTT 1 cut(s) 332
AclWI GGATC 2 cut(s) 482, 495
AcsI RAATTY 1 cut(s) 282
AcyI GRCGYC 1 cut(s) 303
AgsI TTSAA 3 cut(s) 139, 182, 481
AjnI CCWGG 1 cut(s) 483
AluBI AGCT 4 cut(s) 164, 199, 382, 475
AluI AGCT 4 cut(s) 164, 199, 382, 475
Alw21I GWGCWC 1 cut(s) 201
AlwI GGATC 2 cut(s) 482, 495
AoxI GGCC 1 cut(s) 15
ApeKI GCWGC 1 cut(s) 164
ApoI RAATTY 1 cut(s) 282
AspLEI GCGC 1 cut(s) 401
AspS9I GGNCC 1 cut(s) 15
AsuHPI GGTGA 2 cut(s) 193, 373
BamHI GGATCC 1 cut(s) 487
BanII GRGCYC 2 cut(s) 201, 348
Bbv12I GWGCWC 1 cut(s) 201
BbvI GCAGC 1 cut(s) 151
BciT130I CCWGG 1 cut(s) 485
BfaI CTAG 2 cut(s) 60, 275
BfmI CTRYAG 1 cut(s) 413
BfuAI ACCTGC 1 cut(s) 208
BisI GCNGC 1 cut(s) 165
BlsI GCNGC 1 cut(s) 166
Bme1390I CCNGG 1 cut(s) 485
BmgT120I GGNCC 1 cut(s) 15
BmiI GGNNCC 1 cut(s) 489
BmrFI CCNGG 1 cut(s) 485
BmsI GCATC 1 cut(s) 222
BpuEI CTTGAG 3 cut(s) 197, 368, 487
BsaHI GRCGYC 1 cut(s) 303
BseBI CCWGG 1 cut(s) 485
BseXI GCAGC 1 cut(s) 151
BsgI GTGCAG 1 cut(s) 242
BshFI GGCC 1 cut(s) 17
BsiHKAI GWGCWC 1 cut(s) 201
BslFI GGGAC 1 cut(s) 10
BsmFI GGGAC 1 cut(s) 10
BsnI GGCC 1 cut(s) 17
Bsp1286I GDGCHC 2 cut(s) 201, 348
Bsp143I GATC 2 cut(s) 192, 487
BspACI CCGC 1 cut(s) 204
BspANI GGCC 1 cut(s) 17
BspLI GGNNCC 1 cut(s) 489
BspMI ACCTGC 1 cut(s) 208
BspPI GGATC 2 cut(s) 482, 495
BssMI GATC 2 cut(s) 192, 487
BssNI GRCGYC 1 cut(s) 303
Bst2UI CCWGG 1 cut(s) 485
Bst4CI ACNGT 2 cut(s) 120, 523
Bst6I CTCTTC 1 cut(s) 109
BstACI GRCGYC 1 cut(s) 303
BstAPI GCANNNNNTGC 1 cut(s) 122
BstC8I GCNNGC 1 cut(s) 261
BstHHI GCGC 1 cut(s) 401
BstKTI GATC 2 cut(s) 195, 490
BstMBI GATC 2 cut(s) 192, 487
BstMWI GCNNNNNNNGC 3 cut(s) 122, 152, 161
BstNI CCWGG 1 cut(s) 485
BstSCI CCNGG 1 cut(s) 483
BstSFI CTRYAG 1 cut(s) 413
BstV1I GCAGC 1 cut(s) 151
BstX2I RGATCY 1 cut(s) 487
BstYI RGATCY 1 cut(s) 487
BsuRI GGCC 1 cut(s) 17
BtsIMutI CAGTG 1 cut(s) 360
BveI ACCTGC 1 cut(s) 208
Cac8I GCNNGC 1 cut(s) 261
CfoI GCGC 1 cut(s) 401
Cfr13I GGNCC 1 cut(s) 15
CseI GACGC 1 cut(s) 311
CviAII CATG 3 cut(s) 172, 190, 517
CviJI RGCY 8 cut(s) 17, 40, 155, 164, 199, 346, 382, 475
CviKI_1 RGCY 8 cut(s) 17, 40, 155, 164, 199, 346, 382, 475
DpnI GATC 2 cut(s) 194, 489
DpnII GATC 2 cut(s) 192, 487
Eam1104I CTCTTC 1 cut(s) 109
EarI CTCTTC 1 cut(s) 109
Ecl136II GAGCTC 1 cut(s) 199
Eco24I GRGCYC 2 cut(s) 201, 348
Eco53kI GAGCTC 1 cut(s) 199
EcoICRI GAGCTC 1 cut(s) 199
EcoRII CCWGG 1 cut(s) 483
EcoT38I GRGCYC 2 cut(s) 201, 348
FaeI CATG 3 cut(s) 175, 193, 520
FaiI YATR 4 cut(s) 173, 191, 323, 518
FaqI GGGAC 1 cut(s) 10
FatI CATG 3 cut(s) 171, 189, 516
Fnu4HI GCNGC 1 cut(s) 165
FriOI GRGCYC 2 cut(s) 201, 348
Fsp4HI GCNGC 1 cut(s) 165
FspBI CTAG 2 cut(s) 60, 275
GlaI GCGC 1 cut(s) 400
GluI GCNGC 1 cut(s) 165
HaeIII GGCC 1 cut(s) 17
HgaI GACGC 1 cut(s) 311
HhaI GCGC 1 cut(s) 401
Hin1I GRCGYC 1 cut(s) 303
Hin1II CATG 3 cut(s) 175, 193, 520
Hin6I GCGC 1 cut(s) 399
HinP1I GCGC 1 cut(s) 399
HinfI GANTC 2 cut(s) 79, 178
HphI GGTGA 2 cut(s) 193, 373
Hpy188I TCNGA 3 cut(s) 84, 93, 507
HpyAV CCTTC 1 cut(s) 239
HpyCH4III ACNGT 2 cut(s) 120, 523
HpyCH4IV ACGT 1 cut(s) 332
HpyCH4V TGCA 2 cut(s) 125, 223
HpyF10VI GCNNNNNNNGC 3 cut(s) 122, 152, 161
HpySE526I ACGT 1 cut(s) 332
Hsp92I GRCGYC 1 cut(s) 303
Hsp92II CATG 3 cut(s) 175, 193, 520
HspAI GCGC 1 cut(s) 399
Kzo9I GATC 2 cut(s) 192, 487
LmnI GCTCC 2 cut(s) 373, 472
LpnPI CCDG 6 cut(s) 203, 242, 309, 356, 470, 497
Lsp1109I GCAGC 1 cut(s) 151
LweI GCATC 1 cut(s) 222
MaeI CTAG 2 cut(s) 60, 275
MaeII ACGT 1 cut(s) 332
MaeIII GTNAC 1 cut(s) 62
MalI GATC 2 cut(s) 194, 489
MboI GATC 2 cut(s) 192, 487
MboII GAAGA 2 cut(s) 96, 339
MfeI CAATTG 1 cut(s) 402
MflI RGATCY 1 cut(s) 487
MhlI GDGCHC 2 cut(s) 201, 348
MluCI AATT 2 cut(s) 282, 402
MlyI GAGTC 1 cut(s) 73
MnlI CCTC 6 cut(s) 112, 271, 418, 430, 445, 462
MspR9I CCNGG 1 cut(s) 485
MunI CAATTG 1 cut(s) 402
MvaI CCWGG 1 cut(s) 485
MwoI GCNNNNNNNGC 3 cut(s) 122, 152, 161
NdeII GATC 2 cut(s) 192, 487
NlaIII CATG 3 cut(s) 175, 193, 520
NlaIV GGNNCC 1 cut(s) 489
PaqCI CACCTGC 1 cut(s) 208
PcsI WCGNNNNNNNCGW 1 cut(s) 74
PfeI GAWTC 1 cut(s) 178
PkrI GCNGC 1 cut(s) 166
PleI GAGTC 1 cut(s) 73
PpsI GAGTC 1 cut(s) 73
Psp124BI GAGCTC 1 cut(s) 201
Psp1406I AACGTT 1 cut(s) 332
Psp6I CCWGG 1 cut(s) 483
PspGI CCWGG 1 cut(s) 483
PspN4I GGNNCC 1 cut(s) 489
PspPI GGNCC 1 cut(s) 15
PsuI RGATCY 1 cut(s) 487
SacI GAGCTC 1 cut(s) 201
SatI GCNGC 1 cut(s) 165
Sau3AI GATC 2 cut(s) 192, 487
Sau96I GGNCC 1 cut(s) 15
SchI GAGTC 1 cut(s) 73
ScrFI CCNGG 1 cut(s) 485
SduI GDGCHC 2 cut(s) 201, 348
SfaNI GCATC 1 cut(s) 222
SfcI CTRYAG 1 cut(s) 413
SmlI CTYRAG 3 cut(s) 212, 383, 466
SmoI CTYRAG 3 cut(s) 212, 383, 466
Sse9I AATT 2 cut(s) 282, 402
SsiI CCGC 1 cut(s) 204
SspMI CTAG 2 cut(s) 60, 275
SstI GAGCTC 1 cut(s) 201
StyD4I CCNGG 1 cut(s) 483
TaaI ACNGT 2 cut(s) 120, 523
TaiI ACGT 1 cut(s) 335
TaqI TCGA 2 cut(s) 133, 195
TasI AATT 2 cut(s) 282, 402
TfiI GAWTC 1 cut(s) 178
TscAI CASTG 1 cut(s) 360
TseI GCWGC 1 cut(s) 164
TspDTI ATGAA 2 cut(s) 160, 381
TspRI CASTG 1 cut(s) 360
XapI RAATTY 1 cut(s) 282
XcmI CCANNNNNNNNNTGG 1 cut(s) 355
XspI CTAG 2 cut(s) 60, 275
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.