MD06G1222300.v1.1

phosphatase 2C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0005794
Physical Location & Seq
Forward (+)
35387113 .. 35390132
3020 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1222300.v1.1.491

Sequence Viewer

Length: 1035 bp
ATGACTGAAACCGAAACCGCCAATCCAGTCCCGACGAACGAGAGTGCAAGAGAGGAGGCGGAGGCCGCGGCAAAGCAAAAGAACAAGCGGGAAAGCGATTCCAGCGATGCTATTTCAGCTGCCAAGAAGTCGAAAACGGAAGAAGATCAAGACCCAGAAGTTGATATGAGCAAAGGGGAAATGGGTTTTGAAATCGAGGCCGATGCTGCGGAGGATAAGGGGCTGAGGCACGCCATGGAGGACGCTTGGGTTGTGCTCTTGGACGCCGCCGGCGAGGATTTTCCCGGAAATTTGAGATGTGCACATTTTGCAGTTTATGATGGGCATGGAGGTCGATTGGCTGCGGAGTATGCTCAGAAGCATCTGAATACGAATGTGCTTTCGGCCGGATTACCACGTGAGTTGTTGGATATAAAAGCAGCCAAGAAAGCCATACTTGACGGTTTTCGGAAAACTGATGAGTCTCTTGTTCGAGAAAGTGCTGCAGGGGGGTGGCAGGATGGTGCTACAGCTGTATGTGTTTGGATATTAGGAAAAACTGTTTTTGTTGCTAACATTGGGGATACAAAAGCAGTGGTAGCAAGATCATCTCCTGTTGAGGGATTGCAAAATGGTTCTAATGAAGCATGTCCCCTGAAGGCGATTGTTTTGACAAGGGAACACAAAGCCATCTATGCACAGGAGCGTGCTCGTATTCAGAAGGCTGGTGGCAATGTGAGTTCAAATGGACGACTACAGGGGCGCCTTGAAGTTTCTAGGGCCTTTGGAGATCGCCAGTTCAAAAAGGTGGGCGTTATTGCAACCCCGGACATTCATTCTTTTGACCTTACAGAAAGAGAGCACTTCATAATTCTTGGTTGCGACGGCCTCTGGGGGGTAATTGGACCAAGTGATGCTGTTGATTTTGTTCAGAAACTGTTGAAGGAGGGACTACCTGTTGCAACTATAAGCCGTCGTCTTGTAAAAGAAGCTGTGCGAGAGAGGCGCTGTAAAGATAACTGCACCGCGATCATAATCGCGTTCAGGCACAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

345

Amino Acids

37.36

Weight (kDa)

5.98

Isoelectric Point (pI)

45.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 71 - 332 2.6e-53 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 741
AccII CGCG 3 cut(s) 68, 1007, 1019
AciI CCGC 9 cut(s) 18, 59, 66, 68, 88, 209, 267, 344, 1005
AcoI YGGCCR 1 cut(s) 384
AcsI RAATTY 1 cut(s) 289
AcuI CTGAAG 1 cut(s) 656
AcvI CACGTG 1 cut(s) 398
AcyI GRCGYC 2 cut(s) 264, 742
AfiI CCNNNNNNNGG 2 cut(s) 599, 874
AgsI TTSAA 5 cut(s) 191, 723, 749, 781, 922
AluBI AGCT 3 cut(s) 119, 512, 971
AluI AGCT 3 cut(s) 119, 512, 971
Alw21I GWGCWC 4 cut(s) 258, 304, 691, 843
Alw26I GTCTC 1 cut(s) 468
Alw44I GTGCAC 1 cut(s) 300
AlwNI CAGNNNCTG 1 cut(s) 916
AoxI GGCC 5 cut(s) 63, 198, 384, 759, 865
ApaLI GTGCAC 1 cut(s) 300
ApeKI GCWGC 5 cut(s) 119, 206, 341, 419, 482
ApoI RAATTY 1 cut(s) 289
AspLEI GCGC 2 cut(s) 744, 987
AspS9I GGNCC 2 cut(s) 759, 884
AsuC2I CCSGG 2 cut(s) 285, 806
AvaII GGWCC 1 cut(s) 884
BaeGI GKGCMC 1 cut(s) 304
BanI GGYRCC 1 cut(s) 741
BbrPI CACGTG 1 cut(s) 398
Bbv12I GWGCWC 4 cut(s) 258, 304, 691, 843
BbvCI CCTCAGC 1 cut(s) 224
BbvI GCAGC 5 cut(s) 106, 193, 328, 431, 469
BccI CCATC 3 cut(s) 314, 494, 677
BceAI ACGGC 2 cut(s) 880, 936
BcgI CGANNNNNNTGC 4 cut(s) 185, 219, 314, 348
BciVI GTATCC 1 cut(s) 556
BcnI CCSGG 2 cut(s) 285, 806
BcoDI GTCTC 1 cut(s) 468
BfaI CTAG 1 cut(s) 756
BfmI CTRYAG 3 cut(s) 483, 507, 734
BfoI RGCGCY 2 cut(s) 745, 988
BfuI GTATCC 1 cut(s) 556
BisI GCNGC 8 cut(s) 66, 69, 120, 207, 267, 342, 420, 483
BlsI GCNGC 8 cut(s) 67, 70, 121, 208, 268, 343, 421, 484
Bme1390I CCNGG 2 cut(s) 285, 806
Bme18I GGWCC 1 cut(s) 884
BmgT120I GGNCC 2 cut(s) 759, 884
BmiI GGNNCC 1 cut(s) 743
BmrFI CCNGG 2 cut(s) 285, 806
BmsI GCATC 4 cut(s) 97, 193, 370, 883
Bpu10I CCTNAGC 1 cut(s) 224
BpuMI CCSGG 2 cut(s) 285, 806
BsaAI YACGTR 1 cut(s) 398
BsaBI GATNNNNATC 1 cut(s) 1013
BsaHI GRCGYC 2 cut(s) 264, 742
BsaJI CCNNGG 3 cut(s) 66, 234, 804
Bsc4I CCNNNNNNNGG 2 cut(s) 599, 874
Bse118I RCCGGY 1 cut(s) 269
Bse1I ACTGG 2 cut(s) 26, 775
Bse3DI GCAATG 1 cut(s) 718
Bse8I GATNNNNATC 1 cut(s) 1013
BseDI CCNNGG 3 cut(s) 66, 234, 804
BseGI GGATG 1 cut(s) 505
BseJI GATNNNNATC 1 cut(s) 1013
BseLI CCNNNNNNNGG 2 cut(s) 599, 874
BseMI GCAATG 1 cut(s) 718
BseMII CTCAG 2 cut(s) 215, 368
BseNI ACTGG 2 cut(s) 26, 775
BseRI GAGGAG 1 cut(s) 68
BseSI GKGCMC 1 cut(s) 304
BseX3I CGGCCG 1 cut(s) 384
BseXI GCAGC 5 cut(s) 106, 193, 328, 431, 469
BsgI GTGCAG 1 cut(s) 985
Bsh1236I CGCG 3 cut(s) 68, 1007, 1019
Bsh1285I CGRYCG 1 cut(s) 387
BshFI GGCC 5 cut(s) 65, 200, 386, 761, 867
BshNI GGYRCC 1 cut(s) 741
BsiEI CGRYCG 1 cut(s) 387
BsiHKAI GWGCWC 4 cut(s) 258, 304, 691, 843
BsiSI CCGG 4 cut(s) 270, 285, 387, 806
BslFI GGGAC 3 cut(s) 14, 615, 942
BslI CCNNNNNNNGG 2 cut(s) 599, 874
BsmAI GTCTC 1 cut(s) 468
BsmFI GGGAC 3 cut(s) 14, 615, 942
BsnI GGCC 5 cut(s) 65, 200, 386, 761, 867
Bsp1286I GDGCHC 4 cut(s) 258, 304, 691, 843
Bsp143I GATC 4 cut(s) 145, 584, 769, 1008
Bsp19I CCATGG 1 cut(s) 234
BspACI CCGC 9 cut(s) 18, 59, 66, 68, 88, 209, 267, 344, 1005
BspANI GGCC 5 cut(s) 65, 200, 386, 761, 867
BspCNI CTCAG 2 cut(s) 216, 367
BspFNI CGCG 3 cut(s) 68, 1007, 1019
BspLI GGNNCC 1 cut(s) 743
BspMAI CTGCAG 1 cut(s) 487
BspT107I GGYRCC 1 cut(s) 741
BsrDI GCAATG 1 cut(s) 718
BsrFI RCCGGY 1 cut(s) 269
BsrI ACTGG 2 cut(s) 26, 775
BssAI RCCGGY 1 cut(s) 269
BssECI CCNNGG 3 cut(s) 66, 234, 804
BssMI GATC 4 cut(s) 145, 584, 769, 1008
BssNI GRCGYC 2 cut(s) 264, 742
BssT1I CCWWGG 1 cut(s) 234
Bst4CI ACNGT 3 cut(s) 443, 541, 918
BstACI GRCGYC 2 cut(s) 264, 742
BstAPI GCANNNNNTGC 1 cut(s) 308
BstBAI YACGTR 1 cut(s) 398
BstC8I GCNNGC 3 cut(s) 231, 271, 687
BstDEI CTNAG 2 cut(s) 224, 354
BstDSI CCRYGG 2 cut(s) 66, 234
BstF5I GGATG 1 cut(s) 505
BstFNI CGCG 3 cut(s) 68, 1007, 1019
BstH2I RGCGCY 2 cut(s) 745, 988
BstHHI GCGC 2 cut(s) 744, 987
BstKTI GATC 4 cut(s) 148, 587, 772, 1011
BstMAI GTCTC 1 cut(s) 468
BstMBI GATC 4 cut(s) 145, 584, 769, 1008
BstMCI CGRYCG 1 cut(s) 387
BstNSI RCATGY 1 cut(s) 630
BstSCI CCNGG 2 cut(s) 283, 804
BstSFI CTRYAG 3 cut(s) 483, 507, 734
BstSLI GKGCMC 1 cut(s) 304
BstUI CGCG 3 cut(s) 68, 1007, 1019
BstV1I GCAGC 5 cut(s) 106, 193, 328, 431, 469
BstZI CGGCCG 1 cut(s) 384
BsuI GTATCC 1 cut(s) 556
BsuRI GGCC 5 cut(s) 65, 200, 386, 761, 867
BtgI CCRYGG 2 cut(s) 66, 234
BtgZI GCGATG 1 cut(s) 120
BtsCI GGATG 1 cut(s) 505
BtsI GCAGTG 1 cut(s) 579
BtsIMutI CAGTG 1 cut(s) 579
Cac8I GCNNGC 3 cut(s) 231, 271, 687
CaiI CAGNNNCTG 1 cut(s) 916
CfoI GCGC 2 cut(s) 744, 987
Cfr10I RCCGGY 1 cut(s) 269
Cfr13I GGNCC 2 cut(s) 759, 884
Cfr42I CCGCGG 1 cut(s) 69
CseI GACGC 2 cut(s) 251, 272
CspCI CAANNNNNGTGG 4 cut(s) 384, 419, 555, 590
CviAII CATG 3 cut(s) 235, 326, 627
DdeI CTNAG 2 cut(s) 224, 354
DinI GGCGCC 1 cut(s) 743
DpnI GATC 4 cut(s) 147, 586, 771, 1010
DpnII GATC 4 cut(s) 145, 584, 769, 1008
EaeI YGGCCR 1 cut(s) 384
EagI CGGCCG 1 cut(s) 384
EciI GGCGGA 1 cut(s) 74
EclXI CGGCCG 1 cut(s) 384
Eco130I CCWWGG 1 cut(s) 234
Eco47I GGWCC 1 cut(s) 884
Eco52I CGGCCG 1 cut(s) 384
Eco57I CTGAAG 1 cut(s) 656
Eco72I CACGTG 1 cut(s) 398
EcoO109I RGGNCCY 1 cut(s) 759
EcoT14I CCWWGG 1 cut(s) 234
EgeI GGCGCC 1 cut(s) 743
EheI GGCGCC 1 cut(s) 743
ErhI CCWWGG 1 cut(s) 234
FaeI CATG 3 cut(s) 238, 329, 630
FalI AAGNNNNNCTT 2 cut(s) 420, 452
FaqI GGGAC 3 cut(s) 14, 615, 942
FatI CATG 3 cut(s) 234, 325, 626
FauI CCCGC 1 cut(s) 81
Fnu4HI GCNGC 8 cut(s) 66, 69, 120, 207, 267, 342, 420, 483
FokI GGATG 1 cut(s) 512
Fsp4HI GCNGC 8 cut(s) 66, 69, 120, 207, 267, 342, 420, 483
FspBI CTAG 1 cut(s) 756
GlaI GCGC 2 cut(s) 743, 986
GluI GCNGC 8 cut(s) 66, 69, 120, 207, 267, 342, 420, 483
HaeII RGCGCY 2 cut(s) 745, 988
HaeIII GGCC 5 cut(s) 65, 200, 386, 761, 867
HapII CCGG 4 cut(s) 270, 285, 387, 806
HgaI GACGC 2 cut(s) 251, 272
HhaI GCGC 2 cut(s) 744, 987
Hin1I GRCGYC 2 cut(s) 264, 742
Hin1II CATG 3 cut(s) 238, 329, 630
Hin6I GCGC 2 cut(s) 742, 985
HinP1I GCGC 2 cut(s) 742, 985
HinfI GANTC 2 cut(s) 98, 461
HpaII CCGG 4 cut(s) 270, 285, 387, 806
Hpy166II GTNNAC 1 cut(s) 302
Hpy188I TCNGA 5 cut(s) 357, 366, 450, 699, 912
Hpy188III TCNNGA 3 cut(s) 31, 149, 473
Hpy8I GTNNAC 1 cut(s) 302
Hpy99I CGWCG 3 cut(s) 37, 866, 957
HpyAV CCTTC 3 cut(s) 631, 694, 916
HpyCH4III ACNGT 3 cut(s) 443, 541, 918
HpyCH4IV ACGT 1 cut(s) 397
HpyCH4V TGCA 9 cut(s) 47, 302, 311, 485, 607, 677, 800, 941, 1002
HpyF3I CTNAG 2 cut(s) 224, 354
HpySE526I ACGT 1 cut(s) 397
Hsp92I GRCGYC 2 cut(s) 264, 742
Hsp92II CATG 3 cut(s) 238, 329, 630
HspAI GCGC 2 cut(s) 742, 985
KasI GGCGCC 1 cut(s) 741
KroI GCCGGC 1 cut(s) 269
KroNI GCCGGC 1 cut(s) 271
KspI CCGCGG 1 cut(s) 69
Kzo9I GATC 4 cut(s) 145, 584, 769, 1008
LmnI GCTCC 1 cut(s) 682
Lsp1109I GCAGC 5 cut(s) 106, 193, 328, 431, 469
LweI GCATC 4 cut(s) 97, 193, 370, 883
MaeI CTAG 1 cut(s) 756
MaeII ACGT 1 cut(s) 397
MalI GATC 4 cut(s) 147, 586, 771, 1010
MboI GATC 4 cut(s) 145, 584, 769, 1008
MboII GAAGA 2 cut(s) 152, 155
MhlI GDGCHC 4 cut(s) 258, 304, 691, 843
MluCI AATT 3 cut(s) 289, 849, 879
Mly113I GGCGCC 1 cut(s) 742
MlyI GAGTC 1 cut(s) 470
MmeI TCCRAC 1 cut(s) 387
MreI CGCCGGCG 1 cut(s) 269
MroNI GCCGGC 1 cut(s) 269
MspA1I CMGCKG 3 cut(s) 68, 119, 512
MspI CCGG 4 cut(s) 270, 285, 387, 806
MspR9I CCNGG 2 cut(s) 285, 806
MvnI CGCG 3 cut(s) 68, 1007, 1019
NaeI GCCGGC 1 cut(s) 271
NarI GGCGCC 1 cut(s) 742
NciI CCSGG 2 cut(s) 285, 806
NcoI CCATGG 1 cut(s) 234
NdeII GATC 4 cut(s) 145, 584, 769, 1008
NgoMIV GCCGGC 1 cut(s) 269
NlaIII CATG 3 cut(s) 238, 329, 630
NlaIV GGNNCC 1 cut(s) 743
NspI RCATGY 1 cut(s) 630
PcsI WCGNNNNNNNCGW 1 cut(s) 270
PdiI GCCGGC 1 cut(s) 271
PfeI GAWTC 1 cut(s) 98
PfoI TCCNGGA 1 cut(s) 283
PkrI GCNGC 8 cut(s) 67, 70, 121, 208, 268, 343, 421, 484
PleI GAGTC 1 cut(s) 469
PluTI GGCGCC 1 cut(s) 745
PmaCI CACGTG 1 cut(s) 398
PmlI CACGTG 1 cut(s) 398
PpsI GAGTC 1 cut(s) 469
Ppu21I YACGTR 1 cut(s) 398
PspCI CACGTG 1 cut(s) 398
PspN4I GGNNCC 1 cut(s) 743
PspPI GGNCC 2 cut(s) 759, 884
PstI CTGCAG 1 cut(s) 487
PstNI CAGNNNCTG 1 cut(s) 916
PvuII CAGCTG 2 cut(s) 119, 512
SacII CCGCGG 1 cut(s) 69
SatI GCNGC 8 cut(s) 66, 69, 120, 207, 267, 342, 420, 483
Sau3AI GATC 4 cut(s) 145, 584, 769, 1008
Sau96I GGNCC 2 cut(s) 759, 884
SchI GAGTC 1 cut(s) 470
ScrFI CCNGG 2 cut(s) 285, 806
SduI GDGCHC 4 cut(s) 258, 304, 691, 843
SetI ASST 8 cut(s) 121, 334, 400, 514, 789, 828, 937, 973
SfaNI GCATC 4 cut(s) 97, 193, 370, 883
SfcI CTRYAG 3 cut(s) 483, 507, 734
SfoI GGCGCC 1 cut(s) 743
Sfr303I CCGCGG 1 cut(s) 69
SgrAI CRCCGGYG 1 cut(s) 269
SgrBI CCGCGG 1 cut(s) 69
SinI GGWCC 1 cut(s) 884
Sse9I AATT 3 cut(s) 289, 849, 879
SsiI CCGC 9 cut(s) 18, 59, 66, 68, 88, 209, 267, 344, 1005
SspDI GGCGCC 1 cut(s) 741
SspMI CTAG 1 cut(s) 756
StyD4I CCNGG 2 cut(s) 283, 804
StyI CCWWGG 1 cut(s) 234
TaaI ACNGT 3 cut(s) 443, 541, 918
TaiI ACGT 1 cut(s) 400
TaqI TCGA 4 cut(s) 131, 195, 334, 472
TasI AATT 3 cut(s) 289, 849, 879
TauI GCSGC 3 cut(s) 68, 71, 269
TfiI GAWTC 1 cut(s) 98
TscAI CASTG 1 cut(s) 579
TseI GCWGC 5 cut(s) 119, 206, 341, 419, 482
TspDTI ATGAA 3 cut(s) 636, 803, 835
TspGWI ACGGA 1 cut(s) 152
TspRI CASTG 1 cut(s) 579
VneI GTGCAC 1 cut(s) 300
VpaK11BI GGWCC 1 cut(s) 884
XapI RAATTY 1 cut(s) 289
XceI RCATGY 1 cut(s) 630
XspI CTAG 1 cut(s) 756
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.