FvH4_5g22450

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0010290
Physical Location & Seq
Forward (+)
13815011 .. 13817448
2438 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g22450.t1

Sequence Viewer

Length: 723 bp
ATGAAATCATCTGTTCTGATCCGAACCAGTTCCATTGGCCTCGCCGTCTCCGGCTCCCCTAGAGTCTCCCTCTCCCGCTCCGACTCTCTCTCCGGCCTCTTCTCGCCTGAGAAGACCAACGCCGCCGCGTCTCCGAGGATCTGCCTCCAGCTCGACGTCAACAGCCGCCGCGACAGGGAGACCATCCGGCGGGCTCTCTCCGACAGCAATATTCTGCGATCGGACTCCGGTAAGGCGAGGGGCGGAAGGTCGCCGGGGAGCGGTATTCCGGAAGCCGAGATGGAATTTAACGGCGGAAGCAAGACGGTGACGAACGAGAGAGAGGAGCGGAGGAAGATGGGAGCGTATTATCTGGAGATGCTCAAGTCTAATCCCAGTGATCCTCTCCTTCTCAGAAACTACGGCCAGTTTCTGCACGAGGTGGAGAAGGATATGGTGCGAGCTGAGGAGTACTATAGCAGAGCAATATTGGCTAGTCCCGGAGACGGCGAGGTGTTGTCGCTGTACGGGAAGCTGATATGGGAGAGTCAAAGGGATAAGGATCGAGCCAAATCTTACTTTAACCGGGCGGTTTCGGCCTCACCTGACAATAGCATGGTTTTGGGATCCTTTGCAAGCTTCATGTGGGAAGCAGAGGAAGACGAAGAAGATGATGAAGAATTCAACAGTAACAGTACAACTGCTCAAGTCTCATCAGCAGCTGAGCTAGTTCCAGTGTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

241

Amino Acids

26.51

Weight (kDa)

5.12

Isoelectric Point (pI)

57.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_TmcB PF25474 113 - 156 4.8e-07 TmcB, TPR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 159
AccBSI CCGCTC 3 cut(s) 78, 261, 328
AccII CGCG 2 cut(s) 128, 171
AccIII TCCGGA 1 cut(s) 268
AclWI GGATC 6 cut(s) 13, 146, 374, 549, 600, 613
AcoI YGGCCR 1 cut(s) 403
AcsI RAATTY 2 cut(s) 284, 659
AcyI GRCGYC 1 cut(s) 156
AdeI CACNNNGTG 1 cut(s) 421
AfaI GTAC 3 cut(s) 452, 506, 676
AfiI CCNNNNNNNGG 4 cut(s) 175, 189, 260, 485
AgsI TTSAA 1 cut(s) 664
AluBI AGCT 6 cut(s) 151, 443, 514, 618, 701, 706
AluI AGCT 6 cut(s) 151, 443, 514, 618, 701, 706
Alw26I GTCTC 6 cut(s) 52, 70, 135, 173, 477, 694
AlwI GGATC 6 cut(s) 13, 146, 374, 549, 600, 613
AlwNI CAGNNNCTG 2 cut(s) 412, 701
Aor13HI TCCGGA 1 cut(s) 268
AoxI GGCC 4 cut(s) 37, 94, 403, 576
ApeKI GCWGC 1 cut(s) 698
ApoI RAATTY 2 cut(s) 284, 659
Asp700I GAANNNNTTC 1 cut(s) 28
AsuC2I CCSGG 3 cut(s) 255, 480, 566
AsuHPI GGTGA 2 cut(s) 319, 573
BamHI GGATCC 1 cut(s) 605
BanII GRGCYC 1 cut(s) 196
BauI CACGAG 1 cut(s) 416
BbsI GAAGAC 2 cut(s) 119, 645
BbvCI CCTCAGC 1 cut(s) 444
BbvI GCAGC 1 cut(s) 710
BccI CCATC 3 cut(s) 191, 274, 331
BceAI ACGGC 4 cut(s) 29, 307, 418, 502
BcnI CCSGG 3 cut(s) 255, 480, 566
BcoDI GTCTC 6 cut(s) 52, 70, 135, 173, 477, 694
BfaI CTAG 3 cut(s) 60, 474, 707
BfmI CTRYAG 1 cut(s) 454
BisI GCNGC 5 cut(s) 123, 126, 166, 169, 699
BlpI GCTNAGC 1 cut(s) 702
BlsI GCNGC 5 cut(s) 124, 127, 167, 170, 700
BmcAI AGTACT 1 cut(s) 452
Bme1390I CCNGG 3 cut(s) 255, 480, 566
BmiI GGNNCC 2 cut(s) 55, 607
BmrFI CCNGG 3 cut(s) 255, 480, 566
BmrI ACTGGG 1 cut(s) 369
BmsI GCATC 1 cut(s) 348
BmuI ACTGGG 1 cut(s) 369
BpiI GAAGAC 2 cut(s) 119, 645
BplI GAGNNNNNCTC 2 cut(s) 54, 86
BpmI CTGGAG 2 cut(s) 131, 374
Bpu10I CCTNAGC 1 cut(s) 444
Bpu1102I GCTNAGC 1 cut(s) 702
BpuEI CTTGAG 2 cut(s) 347, 669
BpuMI CCSGG 3 cut(s) 255, 480, 566
BsaBI GATNNNNATC 1 cut(s) 540
BsaHI GRCGYC 1 cut(s) 156
BsaI GGTCTC 1 cut(s) 173
BsaJI CCNNGG 2 cut(s) 134, 254
BsaWI WCCGGW 2 cut(s) 227, 268
BsaXI ACNNNNNCTCC 2 cut(s) 74, 104
Bsc4I CCNNNNNNNGG 4 cut(s) 175, 189, 260, 485
Bse1I ACTGG 4 cut(s) 27, 375, 406, 713
Bse8I GATNNNNATC 1 cut(s) 540
BseAI TCCGGA 1 cut(s) 268
BseDI CCNNGG 2 cut(s) 134, 254
BseGI GGATG 1 cut(s) 183
BseJI GATNNNNATC 1 cut(s) 540
BseLI CCNNNNNNNGG 4 cut(s) 175, 189, 260, 485
BseMII CTCAG 4 cut(s) 99, 406, 435, 693
BseNI ACTGG 4 cut(s) 27, 375, 406, 713
BseRI GAGGAG 2 cut(s) 338, 461
BseXI GCAGC 1 cut(s) 710
BsgI GTGCAG 1 cut(s) 398
Bsh1236I CGCG 2 cut(s) 128, 171
Bsh1285I CGRYCG 1 cut(s) 221
BshFI GGCC 4 cut(s) 39, 96, 405, 578
BsiEI CGRYCG 1 cut(s) 221
BsiSI CCGG 8 cut(s) 51, 93, 187, 228, 254, 269, 480, 565
BslFI GGGAC 1 cut(s) 462
BslI CCNNNNNNNGG 4 cut(s) 175, 189, 260, 485
BsmAI GTCTC 6 cut(s) 52, 70, 135, 173, 477, 694
BsmBI CGTCTC 3 cut(s) 52, 135, 477
BsmFI GGGAC 1 cut(s) 462
BsnI GGCC 4 cut(s) 39, 96, 405, 578
Bso31I GGTCTC 1 cut(s) 173
Bsp1286I GDGCHC 1 cut(s) 196
Bsp13I TCCGGA 1 cut(s) 268
Bsp143I GATC 6 cut(s) 18, 138, 218, 379, 541, 605
Bsp1720I GCTNAGC 1 cut(s) 702
BspANI GGCC 4 cut(s) 39, 96, 405, 578
BspCNI CTCAG 4 cut(s) 100, 405, 436, 694
BspEI TCCGGA 1 cut(s) 268
BspFNI CGCG 2 cut(s) 128, 171
BspLI GGNNCC 2 cut(s) 55, 607
BspPI GGATC 6 cut(s) 13, 146, 374, 549, 600, 613
BspTNI GGTCTC 1 cut(s) 173
BsrBI CCGCTC 3 cut(s) 78, 261, 328
BsrI ACTGG 4 cut(s) 27, 375, 406, 713
BssECI CCNNGG 2 cut(s) 134, 254
BssMI GATC 6 cut(s) 18, 138, 218, 379, 541, 605
BssNI GRCGYC 1 cut(s) 156
BssSI CACGAG 1 cut(s) 416
Bst2BI CACGAG 1 cut(s) 416
Bst4CI ACNGT 3 cut(s) 307, 668, 674
Bst6I CTCTTC 1 cut(s) 104
BstACI GRCGYC 1 cut(s) 156
BstC8I GCNNGC 3 cut(s) 192, 441, 616
BstDEI CTNAG 4 cut(s) 108, 392, 444, 702
BstF5I GGATG 1 cut(s) 183
BstFNI CGCG 2 cut(s) 128, 171
BstKTI GATC 6 cut(s) 21, 141, 221, 382, 544, 608
BstMAI GTCTC 6 cut(s) 52, 70, 135, 173, 477, 694
BstMBI GATC 6 cut(s) 18, 138, 218, 379, 541, 605
BstMCI CGRYCG 1 cut(s) 221
BstMWI GCNNNNNNNGC 2 cut(s) 470, 575
BstSCI CCNGG 3 cut(s) 253, 478, 564
BstSFI CTRYAG 1 cut(s) 454
BstUI CGCG 2 cut(s) 128, 171
BstV1I GCAGC 1 cut(s) 710
BstV2I GAAGAC 2 cut(s) 119, 645
BstX2I RGATCY 2 cut(s) 138, 605
BstYI RGATCY 2 cut(s) 138, 605
BsuRI GGCC 4 cut(s) 39, 96, 405, 578
BtsCI GGATG 1 cut(s) 183
BtsIMutI CAGTG 2 cut(s) 382, 720
Cac8I GCNNGC 3 cut(s) 192, 441, 616
CaiI CAGNNNCTG 2 cut(s) 412, 701
CseI GACGC 1 cut(s) 117
Csp6I GTAC 3 cut(s) 451, 505, 675
CviAII CATG 2 cut(s) 595, 622
CviQI GTAC 3 cut(s) 451, 505, 675
DdeI CTNAG 4 cut(s) 108, 392, 444, 702
DpnI GATC 6 cut(s) 20, 140, 220, 381, 543, 607
DpnII GATC 6 cut(s) 18, 138, 218, 379, 541, 605
DraIII CACNNNGTG 1 cut(s) 421
EaeI YGGCCR 1 cut(s) 403
Eam1104I CTCTTC 1 cut(s) 104
EarI CTCTTC 1 cut(s) 104
EciI GGCGGA 2 cut(s) 258, 309
Eco24I GRGCYC 1 cut(s) 196
Eco31I GGTCTC 1 cut(s) 173
EcoRI GAATTC 1 cut(s) 659
EcoT38I GRGCYC 1 cut(s) 196
Esp3I CGTCTC 3 cut(s) 52, 135, 477
FaeI CATG 2 cut(s) 598, 625
FaiI YATR 5 cut(s) 434, 456, 520, 596, 623
FaqI GGGAC 1 cut(s) 462
FatI CATG 2 cut(s) 594, 621
FauI CCCGC 2 cut(s) 83, 183
Fnu4HI GCNGC 5 cut(s) 123, 126, 166, 169, 699
FokI GGATG 1 cut(s) 170
FriOI GRGCYC 1 cut(s) 196
Fsp4HI GCNGC 5 cut(s) 123, 126, 166, 169, 699
FspBI CTAG 3 cut(s) 60, 474, 707
GluI GCNGC 5 cut(s) 123, 126, 166, 169, 699
GsuI CTGGAG 2 cut(s) 131, 374
HaeIII GGCC 4 cut(s) 39, 96, 405, 578
HapII CCGG 8 cut(s) 51, 93, 187, 228, 254, 269, 480, 565
HgaI GACGC 1 cut(s) 117
Hin1I GRCGYC 1 cut(s) 156
Hin1II CATG 2 cut(s) 598, 625
HincII GTYRAC 1 cut(s) 160
HindII GTYRAC 1 cut(s) 160
HindIII AAGCTT 1 cut(s) 616
HinfI GANTC 4 cut(s) 63, 83, 224, 526
HpaII CCGG 8 cut(s) 51, 93, 187, 228, 254, 269, 480, 565
HphI GGTGA 2 cut(s) 319, 573
Hpy166II GTNNAC 1 cut(s) 160
Hpy188I TCNGA 7 cut(s) 18, 23, 82, 135, 202, 223, 395
Hpy188III TCNNGA 2 cut(s) 269, 353
Hpy8I GTNNAC 1 cut(s) 160
Hpy99I CGWCG 1 cut(s) 158
HpyAV CCTTC 3 cut(s) 240, 398, 421
HpyCH4III ACNGT 3 cut(s) 307, 668, 674
HpyCH4IV ACGT 1 cut(s) 156
HpyCH4V TGCA 2 cut(s) 415, 614
HpyF10VI GCNNNNNNNGC 2 cut(s) 470, 575
HpyF3I CTNAG 4 cut(s) 108, 392, 444, 702
HpySE526I ACGT 1 cut(s) 156
Hsp92I GRCGYC 1 cut(s) 156
Hsp92II CATG 2 cut(s) 598, 625
Kpn2I TCCGGA 1 cut(s) 268
Kzo9I GATC 6 cut(s) 18, 138, 218, 379, 541, 605
LmnI GCTCC 5 cut(s) 59, 83, 258, 325, 341
Lsp1109I GCAGC 1 cut(s) 710
LweI GCATC 1 cut(s) 348
MaeI CTAG 3 cut(s) 60, 474, 707
MaeII ACGT 1 cut(s) 156
MaeIII GTNAC 2 cut(s) 307, 668
MalI GATC 6 cut(s) 20, 140, 220, 381, 543, 607
MbiI CCGCTC 3 cut(s) 78, 261, 328
MboI GATC 6 cut(s) 18, 138, 218, 379, 541, 605
MboII GAAGA 7 cut(s) 91, 124, 346, 650, 656, 659, 668
MflI RGATCY 2 cut(s) 138, 605
MhlI GDGCHC 1 cut(s) 196
MluCI AATT 2 cut(s) 284, 659
MlyI GAGTC 4 cut(s) 72, 77, 218, 535
MmeI TCCRAC 2 cut(s) 105, 225
MroI TCCGGA 1 cut(s) 268
MroXI GAANNNNTTC 1 cut(s) 28
MseI TTAA 2 cut(s) 288, 561
MspA1I CMGCKG 1 cut(s) 701
MspI CCGG 8 cut(s) 51, 93, 187, 228, 254, 269, 480, 565
MspR9I CCNGG 3 cut(s) 255, 480, 566
MvnI CGCG 2 cut(s) 128, 171
MwoI GCNNNNNNNGC 2 cut(s) 470, 575
NciI CCSGG 3 cut(s) 255, 480, 566
NdeII GATC 6 cut(s) 18, 138, 218, 379, 541, 605
NlaIII CATG 2 cut(s) 598, 625
NlaIV GGNNCC 2 cut(s) 55, 607
NmeAIII GCCGAG 1 cut(s) 301
NmuCI GTSAC 1 cut(s) 307
PdmI GAANNNNTTC 1 cut(s) 28
PfoI TCCNGGA 1 cut(s) 478
PkrI GCNGC 5 cut(s) 124, 127, 167, 170, 700
Ple19I CGATCG 1 cut(s) 221
PleI GAGTC 4 cut(s) 71, 77, 218, 534
PpsI GAGTC 4 cut(s) 71, 77, 218, 534
PspN4I GGNNCC 2 cut(s) 55, 607
PstNI CAGNNNCTG 2 cut(s) 412, 701
PsuI RGATCY 2 cut(s) 138, 605
PvuI CGATCG 1 cut(s) 221
PvuII CAGCTG 1 cut(s) 701
RsaI GTAC 3 cut(s) 452, 506, 676
RsaNI GTAC 3 cut(s) 451, 505, 675
SaqAI TTAA 2 cut(s) 288, 561
SatI GCNGC 5 cut(s) 123, 126, 166, 169, 699
Sau3AI GATC 6 cut(s) 18, 138, 218, 379, 541, 605
ScaI AGTACT 1 cut(s) 452
SchI GAGTC 4 cut(s) 72, 77, 218, 535
ScrFI CCNGG 3 cut(s) 255, 480, 566
SduI GDGCHC 1 cut(s) 196
SfaNI GCATC 1 cut(s) 348
SfcI CTRYAG 1 cut(s) 454
SmlI CTYRAG 2 cut(s) 362, 684
SmoI CTYRAG 2 cut(s) 362, 684
Sse9I AATT 2 cut(s) 284, 659
SspI AATATT 2 cut(s) 211, 468
SspMI CTAG 3 cut(s) 60, 474, 707
StyD4I CCNGG 3 cut(s) 253, 478, 564
TaaI ACNGT 3 cut(s) 307, 668, 674
TaiI ACGT 1 cut(s) 159
TaqI TCGA 2 cut(s) 153, 544
TasI AATT 2 cut(s) 284, 659
TatI WGTACW 2 cut(s) 450, 674
TauI GCSGC 4 cut(s) 125, 128, 168, 171
Tru1I TTAA 2 cut(s) 288, 561
Tru9I TTAA 2 cut(s) 288, 561
TscAI CASTG 2 cut(s) 382, 720
TseFI GTSAC 1 cut(s) 307
TseI GCWGC 1 cut(s) 698
Tsp45I GTSAC 1 cut(s) 307
TspDTI ATGAA 3 cut(s) 17, 610, 669
TspRI CASTG 2 cut(s) 382, 720
XapI RAATTY 2 cut(s) 284, 659
XmnI GAANNNNTTC 1 cut(s) 28
XspI CTAG 3 cut(s) 60, 474, 707
ZraI GACGTC 1 cut(s) 157
ZrmI AGTACT 1 cut(s) 452
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.