MD06G1036900.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0010290
Physical Location & Seq
Forward (+)
4699553 .. 4701512
1960 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1036900.v1.1.491

Sequence Viewer

Length: 741 bp
ATGATCCGAACCGGTTCGATTGCGCTTCAGTCACCGGCCGTTTCCGGCTCGCCCAGAGTGTCCCTAGCCCGGAACGACTCGCTCTCGAGCGTTTTCTCCGGCGAGAGGAGCACCGTCGGATCTCCCATGATCTCCCTGCATTTCGACGTCAATCGCCGCCGAGATAAGACGATCCAGAGAGCTCTGTCGGAGAGCGACATTCTCCGATCGGCGCCCGACGGATTTCGAGCTCTGCCGGCGAGTTACGCCGGAAAATTGGCGGGAAGTGGAATTTCGACGGAGGAGCTGGTTCCTGGCGGCGGCGATCGCGGAGGGAAAACTGTAACGGTGGAGGAAGGCGAGCAGAGGAAGATCGGAGCTTATTATCAAGAGATGCTGAAGTCAAACCCCGGCGATCCGTTGCTGCTGAGGAACTACGGGAAATTCTTGCACGAGGTGGAGAAGGATGCGGTGGGGGCGGAGGAATGCTATTGTAGGGCGATATTGGGGAGTCCGGGAGACGGTGAGTTGTTGTCGCTCTACGCAAAGCTAATCTGGGAGACGCAGAGAGATGAGGAGCGGGCTAAATCGTACTTTGATCAGGCTGTTTCTGCCTCCCCTGATGATTGCATGGTGTTGGGGTCCTTCGCTAGCTTCATGTGGGAAGCAGAGGAAGACGAAGACGAAGACGACAGCAAAGAAATCAACATTTACGATGGAACTCAAGTCTCATCGGTGCCGGCTCTGGTTCCTGCATTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

247

Amino Acids

26.69

Weight (kDa)

4.66

Isoelectric Point (pI)

58.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_TmcB PF25474 117 - 160 4.3e-06 TmcB, TPR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 150
AccB1I GGYRCC 2 cut(s) 211, 715
AccBSI CCGCTC 1 cut(s) 559
AccII CGCG 1 cut(s) 309
AciI CCGC 8 cut(s) 157, 260, 297, 300, 309, 449, 458, 559
AclWI GGATC 3 cut(s) 127, 166, 389
AcoI YGGCCR 1 cut(s) 36
AcsI RAATTY 2 cut(s) 270, 422
AcuI CTGAAG 2 cut(s) 11, 398
AcyI GRCGYC 2 cut(s) 147, 212
AdeI CACNNNGTG 1 cut(s) 436
AfaI GTAC 1 cut(s) 572
AfiI CCNNNNNNNGG 4 cut(s) 69, 105, 299, 500
AgeI ACCGGT 1 cut(s) 11
AjnI CCWGG 1 cut(s) 292
AluBI AGCT 6 cut(s) 182, 230, 286, 359, 529, 633
AluI AGCT 6 cut(s) 182, 230, 286, 359, 529, 633
Alw21I GWGCWC 3 cut(s) 113, 184, 232
Alw26I GTCTC 3 cut(s) 492, 533, 712
AlwI GGATC 3 cut(s) 127, 166, 389
Ama87I CYCGRG 1 cut(s) 85
AoxI GGCC 1 cut(s) 36
ApeKI GCWGC 1 cut(s) 403
ApoI RAATTY 2 cut(s) 270, 422
AsiGI ACCGGT 1 cut(s) 11
AsiSI GCGATCGC 1 cut(s) 307
Asp700I GAANNNNTTC 1 cut(s) 13
AspLEI GCGC 2 cut(s) 25, 214
AspS9I GGNCC 1 cut(s) 621
AsuC2I CCSGG 3 cut(s) 70, 390, 495
AsuHPI GGTGA 2 cut(s) 24, 515
AsuNHI GCTAGC 1 cut(s) 629
AvaI CYCGRG 1 cut(s) 85
AvaII GGWCC 1 cut(s) 621
BanI GGYRCC 2 cut(s) 211, 715
BanII GRGCYC 2 cut(s) 184, 232
BauI CACGAG 1 cut(s) 431
BbsI GAAGAC 3 cut(s) 660, 666, 672
Bbv12I GWGCWC 3 cut(s) 113, 184, 232
BbvCI CCTCAGC 1 cut(s) 407
BbvI GCAGC 1 cut(s) 390
BccI CCATC 1 cut(s) 689
BceAI ACGGC 1 cut(s) 23
BciT130I CCWGG 1 cut(s) 294
BclI TGATCA 1 cut(s) 577
BcnI CCSGG 3 cut(s) 70, 390, 495
BcoDI GTCTC 3 cut(s) 492, 533, 712
BfaI CTAG 2 cut(s) 65, 630
BfoI RGCGCY 1 cut(s) 215
BisI GCNGC 4 cut(s) 157, 298, 301, 404
BlsI GCNGC 4 cut(s) 158, 299, 302, 405
Bme1390I CCNGG 4 cut(s) 70, 294, 390, 495
Bme18I GGWCC 1 cut(s) 621
BmeT110I CYCGRG 1 cut(s) 85
BmgT120I GGNCC 1 cut(s) 621
BmiI GGNNCC 5 cut(s) 213, 291, 622, 717, 729
BmrFI CCNGG 4 cut(s) 70, 294, 390, 495
BmsI GCATC 2 cut(s) 363, 436
BmtI GCTAGC 1 cut(s) 633
BpiI GAAGAC 3 cut(s) 660, 666, 672
Bpu10I CCTNAGC 1 cut(s) 407
BpuEI CTTGAG 1 cut(s) 687
BpuMI CCSGG 3 cut(s) 70, 390, 495
BsaHI GRCGYC 2 cut(s) 147, 212
BsaJI CCNNGG 1 cut(s) 388
BsaWI WCCGGW 1 cut(s) 11
Bsc4I CCNNNNNNNGG 4 cut(s) 69, 105, 299, 500
Bse118I RCCGGY 4 cut(s) 11, 34, 235, 718
BseBI CCWGG 1 cut(s) 294
BseDI CCNNGG 1 cut(s) 388
BseGI GGATG 1 cut(s) 451
BseLI CCNNNNNNNGG 4 cut(s) 69, 105, 299, 500
BseMII CTCAG 1 cut(s) 398
BseRI GAGGAG 3 cut(s) 121, 296, 569
BseX3I CGGCCG 1 cut(s) 36
BseXI GCAGC 1 cut(s) 390
Bsh1236I CGCG 1 cut(s) 309
Bsh1285I CGRYCG 3 cut(s) 39, 209, 307
BshFI GGCC 1 cut(s) 38
BshNI GGYRCC 2 cut(s) 211, 715
BshTI ACCGGT 1 cut(s) 11
BsiEI CGRYCG 3 cut(s) 39, 209, 307
BsiHKAI GWGCWC 3 cut(s) 113, 184, 232
BsiHKCI CYCGRG 1 cut(s) 85
BslFI GGGAC 1 cut(s) 46
BslI CCNNNNNNNGG 4 cut(s) 69, 105, 299, 500
BsmAI GTCTC 3 cut(s) 492, 533, 712
BsmBI CGTCTC 2 cut(s) 492, 533
BsmFI GGGAC 1 cut(s) 46
BsmI GAATGC 1 cut(s) 470
BsnI GGCC 1 cut(s) 38
BsoBI CYCGRG 1 cut(s) 85
Bsp1286I GDGCHC 3 cut(s) 113, 184, 232
Bsp143I GATC 9 cut(s) 3, 119, 129, 171, 206, 304, 351, 394, 577
BspACI CCGC 8 cut(s) 157, 260, 297, 300, 309, 449, 458, 559
BspANI GGCC 1 cut(s) 38
BspCNI CTCAG 1 cut(s) 399
BspFNI CGCG 1 cut(s) 309
BspLI GGNNCC 5 cut(s) 213, 291, 622, 717, 729
BspOI GCTAGC 1 cut(s) 633
BspPI GGATC 3 cut(s) 127, 166, 389
BspT107I GGYRCC 2 cut(s) 211, 715
BsrBI CCGCTC 1 cut(s) 559
BsrFI RCCGGY 4 cut(s) 11, 34, 235, 718
BssAI RCCGGY 4 cut(s) 11, 34, 235, 718
BssECI CCNNGG 1 cut(s) 388
BssMI GATC 9 cut(s) 3, 119, 129, 171, 206, 304, 351, 394, 577
BssNI GRCGYC 2 cut(s) 147, 212
BssSI CACGAG 1 cut(s) 431
Bst2BI CACGAG 1 cut(s) 431
Bst2UI CCWGG 1 cut(s) 294
Bst4CI ACNGT 4 cut(s) 115, 322, 328, 503
BstACI GRCGYC 2 cut(s) 147, 212
BstC8I GCNNGC 6 cut(s) 50, 237, 341, 561, 631, 720
BstDEI CTNAG 1 cut(s) 407
BstF5I GGATG 1 cut(s) 451
BstFNI CGCG 1 cut(s) 309
BstH2I RGCGCY 1 cut(s) 215
BstHHI GCGC 2 cut(s) 25, 214
BstKTI GATC 9 cut(s) 6, 122, 132, 174, 209, 307, 354, 397, 580
BstMAI GTCTC 3 cut(s) 492, 533, 712
BstMBI GATC 9 cut(s) 3, 119, 129, 171, 206, 304, 351, 394, 577
BstMCI CGRYCG 3 cut(s) 39, 209, 307
BstMWI GCNNNNNNNGC 6 cut(s) 108, 236, 245, 306, 455, 590
BstNI CCWGG 1 cut(s) 294
BstSCI CCNGG 4 cut(s) 68, 292, 388, 493
BstUI CGCG 1 cut(s) 309
BstV1I GCAGC 1 cut(s) 390
BstV2I GAAGAC 3 cut(s) 660, 666, 672
BstX2I RGATCY 1 cut(s) 119
BstYI RGATCY 1 cut(s) 119
BstZI CGGCCG 1 cut(s) 36
BsuRI GGCC 1 cut(s) 38
BtsCI GGATG 1 cut(s) 451
Cac8I GCNNGC 6 cut(s) 50, 237, 341, 561, 631, 720
CfoI GCGC 2 cut(s) 25, 214
Cfr10I RCCGGY 4 cut(s) 11, 34, 235, 718
Cfr13I GGNCC 1 cut(s) 621
CseI GACGC 1 cut(s) 550
Csp6I GTAC 1 cut(s) 571
CspAI ACCGGT 1 cut(s) 11
CviAII CATG 3 cut(s) 127, 610, 637
CviQI GTAC 1 cut(s) 571
DdeI CTNAG 1 cut(s) 407
DinI GGCGCC 1 cut(s) 213
DpnI GATC 9 cut(s) 5, 121, 131, 173, 208, 306, 353, 396, 579
DpnII GATC 9 cut(s) 3, 119, 129, 171, 206, 304, 351, 394, 577
DraIII CACNNNGTG 1 cut(s) 436
EaeI YGGCCR 1 cut(s) 36
EagI CGGCCG 1 cut(s) 36
EciI GGCGGA 1 cut(s) 473
Ecl136II GAGCTC 2 cut(s) 182, 230
EclXI CGGCCG 1 cut(s) 36
Eco24I GRGCYC 2 cut(s) 184, 232
Eco47I GGWCC 1 cut(s) 621
Eco52I CGGCCG 1 cut(s) 36
Eco53kI GAGCTC 2 cut(s) 182, 230
Eco57I CTGAAG 2 cut(s) 11, 398
Eco88I CYCGRG 1 cut(s) 85
EcoICRI GAGCTC 2 cut(s) 182, 230
EcoO109I RGGNCCY 1 cut(s) 621
EcoRII CCWGG 1 cut(s) 292
EcoT38I GRGCYC 2 cut(s) 184, 232
EgeI GGCGCC 1 cut(s) 213
EheI GGCGCC 1 cut(s) 213
Esp3I CGTCTC 2 cut(s) 492, 533
FaeI CATG 3 cut(s) 130, 613, 640
FaiI YATR 3 cut(s) 128, 611, 638
FaqI GGGAC 1 cut(s) 46
FatI CATG 3 cut(s) 126, 609, 636
FauI CCCGC 2 cut(s) 253, 552
FbaI TGATCA 1 cut(s) 577
Fnu4HI GCNGC 4 cut(s) 157, 298, 301, 404
FokI GGATG 1 cut(s) 458
FriOI GRGCYC 2 cut(s) 184, 232
Fsp4HI GCNGC 4 cut(s) 157, 298, 301, 404
FspBI CTAG 2 cut(s) 65, 630
GlaI GCGC 2 cut(s) 24, 213
GluI GCNGC 4 cut(s) 157, 298, 301, 404
HaeII RGCGCY 1 cut(s) 215
HaeIII GGCC 1 cut(s) 38
HgaI GACGC 1 cut(s) 550
HhaI GCGC 2 cut(s) 25, 214
Hin1I GRCGYC 2 cut(s) 147, 212
Hin1II CATG 3 cut(s) 130, 613, 640
Hin6I GCGC 2 cut(s) 23, 212
HinP1I GCGC 2 cut(s) 23, 212
HinfI GANTC 2 cut(s) 77, 490
HphI GGTGA 2 cut(s) 24, 515
Hpy188I TCNGA 5 cut(s) 8, 119, 190, 206, 356
Hpy188III TCNNGA 3 cut(s) 85, 175, 368
Hpy99I CGWCG 4 cut(s) 119, 149, 221, 280
HpyAV CCTTC 3 cut(s) 329, 436, 634
HpyCH4III ACNGT 4 cut(s) 115, 322, 328, 503
HpyCH4IV ACGT 1 cut(s) 147
HpyCH4V TGCA 4 cut(s) 139, 430, 609, 734
HpyF10VI GCNNNNNNNGC 6 cut(s) 108, 236, 245, 306, 455, 590
HpyF3I CTNAG 1 cut(s) 407
HpySE526I ACGT 1 cut(s) 147
Hsp92I GRCGYC 2 cut(s) 147, 212
Hsp92II CATG 3 cut(s) 130, 613, 640
HspAI GCGC 2 cut(s) 23, 212
KasI GGCGCC 1 cut(s) 211
KroI GCCGGC 2 cut(s) 235, 718
KroNI GCCGGC 2 cut(s) 237, 720
Ksp22I TGATCA 1 cut(s) 577
Kzo9I GATC 9 cut(s) 3, 119, 129, 171, 206, 304, 351, 394, 577
LmnI GCTCC 4 cut(s) 108, 283, 356, 556
Lsp1109I GCAGC 1 cut(s) 390
LweI GCATC 2 cut(s) 363, 436
MaeI CTAG 2 cut(s) 65, 630
MaeII ACGT 1 cut(s) 147
MaeIII GTNAC 3 cut(s) 30, 242, 322
MalI GATC 9 cut(s) 5, 121, 131, 173, 208, 306, 353, 396, 579
MbiI CCGCTC 1 cut(s) 559
MboI GATC 9 cut(s) 3, 119, 129, 171, 206, 304, 351, 394, 577
MboII GAAGA 4 cut(s) 361, 665, 671, 677
MflI RGATCY 1 cut(s) 119
MhlI GDGCHC 3 cut(s) 113, 184, 232
MluCI AATT 3 cut(s) 254, 270, 422
Mly113I GGCGCC 1 cut(s) 212
MlyI GAGTC 2 cut(s) 71, 499
MmeI TCCRAC 2 cut(s) 97, 168
MroNI GCCGGC 2 cut(s) 235, 718
MroXI GAANNNNTTC 1 cut(s) 13
MseI TTAA 1 cut(s) 739
MspR9I CCNGG 4 cut(s) 70, 294, 390, 495
Mva1269I GAATGC 1 cut(s) 470
MvaI CCWGG 1 cut(s) 294
MvnI CGCG 1 cut(s) 309
MwoI GCNNNNNNNGC 6 cut(s) 108, 236, 245, 306, 455, 590
NaeI GCCGGC 2 cut(s) 237, 720
NarI GGCGCC 1 cut(s) 212
NciI CCSGG 3 cut(s) 70, 390, 495
NdeII GATC 9 cut(s) 3, 119, 129, 171, 206, 304, 351, 394, 577
NgoMIV GCCGGC 2 cut(s) 235, 718
NheI GCTAGC 1 cut(s) 629
NlaIII CATG 3 cut(s) 130, 613, 640
NlaIV GGNNCC 5 cut(s) 213, 291, 622, 717, 729
NmeAIII GCCGAG 1 cut(s) 185
NmuCI GTSAC 1 cut(s) 30
PaeR7I CTCGAG 1 cut(s) 85
PctI GAATGC 1 cut(s) 470
PdiI GCCGGC 2 cut(s) 237, 720
PdmI GAANNNNTTC 1 cut(s) 13
PfoI TCCNGGA 1 cut(s) 493
PinAI ACCGGT 1 cut(s) 11
PkrI GCNGC 4 cut(s) 158, 299, 302, 405
Ple19I CGATCG 2 cut(s) 209, 307
PleI GAGTC 2 cut(s) 71, 498
PluTI GGCGCC 1 cut(s) 215
PpsI GAGTC 2 cut(s) 71, 498
PpuMI RGGWCCY 1 cut(s) 621
Psp124BI GAGCTC 2 cut(s) 184, 232
Psp5II RGGWCCY 1 cut(s) 621
Psp6I CCWGG 1 cut(s) 292
PspGI CCWGG 1 cut(s) 292
PspN4I GGNNCC 5 cut(s) 213, 291, 622, 717, 729
PspPI GGNCC 1 cut(s) 621
PspPPI RGGWCCY 1 cut(s) 621
PsuI RGATCY 1 cut(s) 119
PvuI CGATCG 2 cut(s) 209, 307
RgaI GCGATCGC 1 cut(s) 307
RsaI GTAC 1 cut(s) 572
RsaNI GTAC 1 cut(s) 571
SacI GAGCTC 2 cut(s) 184, 232
SaqAI TTAA 1 cut(s) 739
SatI GCNGC 4 cut(s) 157, 298, 301, 404
Sau3AI GATC 9 cut(s) 3, 119, 129, 171, 206, 304, 351, 394, 577
Sau96I GGNCC 1 cut(s) 621
SchI GAGTC 2 cut(s) 71, 499
ScrFI CCNGG 4 cut(s) 70, 294, 390, 495
SduI GDGCHC 3 cut(s) 113, 184, 232
SetI ASST 8 cut(s) 150, 184, 232, 288, 361, 438, 531, 635
SfaAI GCGATCGC 1 cut(s) 307
SfaNI GCATC 2 cut(s) 363, 436
SfoI GGCGCC 1 cut(s) 213
Sfr274I CTCGAG 1 cut(s) 85
SgfI GCGATCGC 1 cut(s) 307
SinI GGWCC 1 cut(s) 621
SlaI CTCGAG 1 cut(s) 85
SmlI CTYRAG 2 cut(s) 85, 702
SmoI CTYRAG 2 cut(s) 85, 702
Sse9I AATT 3 cut(s) 254, 270, 422
SsiI CCGC 8 cut(s) 157, 260, 297, 300, 309, 449, 458, 559
SspDI GGCGCC 1 cut(s) 211
SspMI CTAG 2 cut(s) 65, 630
SstI GAGCTC 2 cut(s) 184, 232
StyD4I CCNGG 4 cut(s) 68, 292, 388, 493
TaaI ACNGT 4 cut(s) 115, 322, 328, 503
TaiI ACGT 1 cut(s) 150
TaqI TCGA 5 cut(s) 17, 86, 144, 226, 275
TasI AATT 3 cut(s) 254, 270, 422
TauI GCSGC 3 cut(s) 159, 300, 303
Tru1I TTAA 1 cut(s) 739
Tru9I TTAA 1 cut(s) 739
TseFI GTSAC 1 cut(s) 30
TseI GCWGC 1 cut(s) 403
Tsp45I GTSAC 1 cut(s) 30
TspDTI ATGAA 1 cut(s) 625
TspGWI ACGGA 3 cut(s) 234, 293, 387
VpaK11BI GGWCC 1 cut(s) 621
XapI RAATTY 2 cut(s) 270, 422
XhoI CTCGAG 1 cut(s) 85
XmnI GAANNNNTTC 1 cut(s) 13
XspI CTAG 2 cut(s) 65, 630
ZraI GACGTC 1 cut(s) 148
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.