Rmu_sc0027080.1_g000001

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0027080.1
OG0010290
Physical Location & Seq
Forward (+)
1 .. 535
535 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0027080.1_g000001.1.cds

Sequence Viewer

Length: 412 bp
cgcagcctctctgagggtttgtctccacttcgacgtcaaccgcagccgcgacaagaacactattcggcgagctgtatccgacagcaacattctacgaccggactccggtaaagagaggtatatcccgtccggaagcaggtccaggtctccgggaagtggaattccggaagaggagatgggaagcaaaaaggtgacgatcgaaagagaagagcggagaaagatgggagagtattatcaggcgatgctcaagtctaatccgagtgatcctctgctgctcagaaactacggtcaattcctgcacgaggtggagaaggatatggtgcgagccgatgagtactattgcagagccatattggcgagtcccggagacggcgagttgttgtcgctgtacgggaagctgatatgggagagt
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

137

Amino Acids

15.64

Weight (kDa)

8.58

Isoelectric Point (pI)

64.64

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 37
Acc36I ACCTGC 1 cut(s) 127
AccBSI CCGCTC 1 cut(s) 212
AccII CGCG 1 cut(s) 49
AccIII TCCGGA 2 cut(s) 129, 164
AciI CCGC 3 cut(s) 41, 47, 212
AclWI GGATC 1 cut(s) 258
AcsI RAATTY 1 cut(s) 160
AcyI GRCGYC 1 cut(s) 34
AdeI CACNNNGTG 1 cut(s) 305
AfaI GTAC 2 cut(s) 336, 390
AfiI CCNNNNNNNGG 6 cut(s) 13, 105, 136, 156, 302, 369
AjnI CCWGG 1 cut(s) 141
AluBI AGCT 2 cut(s) 72, 398
AluI AGCT 2 cut(s) 72, 398
Alw26I GTCTC 3 cut(s) 27, 151, 361
AlwI GGATC 1 cut(s) 258
Aor13HI TCCGGA 2 cut(s) 129, 164
ApeKI GCWGC 3 cut(s) 3, 43, 272
ApoI RAATTY 1 cut(s) 160
AspS9I GGNCC 1 cut(s) 139
AsuC2I CCSGG 2 cut(s) 151, 364
AsuHPI GGTGA 1 cut(s) 203
AvaII GGWCC 1 cut(s) 139
BauI CACGAG 1 cut(s) 300
BbvI GCAGC 3 cut(s) 15, 55, 259
BccI CCATC 2 cut(s) 170, 215
BceAI ACGGC 1 cut(s) 386
BciT130I CCWGG 1 cut(s) 143
BciVI GTATCC 1 cut(s) 86
BcnI CCSGG 2 cut(s) 151, 364
BcoDI GTCTC 3 cut(s) 27, 151, 361
BfuAI ACCTGC 1 cut(s) 127
BfuI GTATCC 1 cut(s) 86
BglI GCCNNNNNGGC 1 cut(s) 354
BisI GCNGC 4 cut(s) 4, 44, 47, 273
BlsI GCNGC 4 cut(s) 5, 45, 48, 274
BmcAI AGTACT 1 cut(s) 336
Bme1390I CCNGG 3 cut(s) 143, 151, 364
Bme18I GGWCC 1 cut(s) 139
BmgT120I GGNCC 1 cut(s) 139
BmrFI CCNGG 3 cut(s) 143, 151, 364
BmsI GCATC 1 cut(s) 232
BplI GAGNNNNNCTC 2 cut(s) 251, 283
BpuEI CTTGAG 1 cut(s) 231
BpuMI CCSGG 2 cut(s) 151, 364
BsaHI GRCGYC 1 cut(s) 34
BsaI GGTCTC 1 cut(s) 151
BsaWI WCCGGW 4 cut(s) 98, 105, 129, 164
Bsc4I CCNNNNNNNGG 6 cut(s) 13, 105, 136, 156, 302, 369
BseAI TCCGGA 2 cut(s) 129, 164
BseBI CCWGG 1 cut(s) 143
BseLI CCNNNNNNNGG 6 cut(s) 13, 105, 136, 156, 302, 369
BseMII CTCAG 1 cut(s) 290
BseRI GAGGAG 1 cut(s) 186
BseXI GCAGC 3 cut(s) 15, 55, 259
BsgI GTGCAG 1 cut(s) 282
Bsh1236I CGCG 1 cut(s) 49
Bsh1285I CGRYCG 2 cut(s) 99, 199
BsiEI CGRYCG 2 cut(s) 99, 199
BsiSI CCGG 6 cut(s) 99, 106, 130, 150, 165, 364
BslFI GGGAC 1 cut(s) 346
BslI CCNNNNNNNGG 6 cut(s) 13, 105, 136, 156, 302, 369
BsmAI GTCTC 3 cut(s) 27, 151, 361
BsmBI CGTCTC 1 cut(s) 361
BsmFI GGGAC 1 cut(s) 346
Bso31I GGTCTC 1 cut(s) 151
Bsp13I TCCGGA 2 cut(s) 129, 164
Bsp143I GATC 2 cut(s) 196, 263
BspACI CCGC 3 cut(s) 41, 47, 212
BspCNI CTCAG 2 cut(s) 4, 289
BspEI TCCGGA 2 cut(s) 129, 164
BspFNI CGCG 1 cut(s) 49
BspMI ACCTGC 1 cut(s) 127
BspPI GGATC 1 cut(s) 258
BspQI GCTCTTC 1 cut(s) 202
BspTNI GGTCTC 1 cut(s) 151
BsrBI CCGCTC 1 cut(s) 212
BssMI GATC 2 cut(s) 196, 263
BssNI GRCGYC 1 cut(s) 34
BssSI CACGAG 1 cut(s) 300
Bst2BI CACGAG 1 cut(s) 300
Bst2UI CCWGG 1 cut(s) 143
Bst4CI ACNGT 1 cut(s) 288
Bst6I CTCTTC 2 cut(s) 163, 202
BstACI GRCGYC 1 cut(s) 34
BstC8I GCNNGC 2 cut(s) 70, 325
BstDEI CTNAG 2 cut(s) 12, 276
BstENI CCTNNNNNAGG 2 cut(s) 11, 300
BstFNI CGCG 1 cut(s) 49
BstKTI GATC 2 cut(s) 199, 266
BstMAI GTCTC 3 cut(s) 27, 151, 361
BstMBI GATC 2 cut(s) 196, 263
BstMCI CGRYCG 2 cut(s) 99, 199
BstMWI GCNNNNNNNGC 1 cut(s) 354
BstNI CCWGG 1 cut(s) 143
BstSCI CCNGG 3 cut(s) 141, 149, 362
BstUI CGCG 1 cut(s) 49
BstV1I GCAGC 3 cut(s) 15, 55, 259
BsuI GTATCC 1 cut(s) 86
BtgZI GCGATG 1 cut(s) 255
BveI ACCTGC 1 cut(s) 127
Cac8I GCNNGC 2 cut(s) 70, 325
Cfr13I GGNCC 1 cut(s) 139
Csp6I GTAC 2 cut(s) 335, 389
CviJI RGCY 6 cut(s) 6, 46, 72, 327, 348, 398
CviKI_1 RGCY 6 cut(s) 6, 46, 72, 327, 348, 398
CviQI GTAC 2 cut(s) 335, 389
DdeI CTNAG 2 cut(s) 12, 276
DpnI GATC 2 cut(s) 198, 265
DpnII GATC 2 cut(s) 196, 263
DraIII CACNNNGTG 1 cut(s) 305
Eam1104I CTCTTC 2 cut(s) 163, 202
EarI CTCTTC 2 cut(s) 163, 202
Eco31I GGTCTC 1 cut(s) 151
Eco47I GGWCC 1 cut(s) 139
EcoNI CCTNNNNNAGG 2 cut(s) 11, 300
EcoRI GAATTC 1 cut(s) 160
EcoRII CCWGG 1 cut(s) 141
Esp3I CGTCTC 1 cut(s) 361
FaiI YATR 4 cut(s) 121, 318, 351, 404
FaqI GGGAC 1 cut(s) 346
Fnu4HI GCNGC 4 cut(s) 4, 44, 47, 273
Fsp4HI GCNGC 4 cut(s) 4, 44, 47, 273
GluI GCNGC 4 cut(s) 4, 44, 47, 273
HapII CCGG 6 cut(s) 99, 106, 130, 150, 165, 364
Hin1I GRCGYC 1 cut(s) 34
HincII GTYRAC 1 cut(s) 38
HindII GTYRAC 1 cut(s) 38
HinfI GANTC 2 cut(s) 102, 359
HpaII CCGG 6 cut(s) 99, 106, 130, 150, 165, 364
HphI GGTGA 1 cut(s) 203
Hpy166II GTNNAC 1 cut(s) 38
Hpy188I TCNGA 4 cut(s) 13, 80, 259, 279
Hpy188III TCNNGA 2 cut(s) 130, 165
Hpy8I GTNNAC 1 cut(s) 38
Hpy99I CGWCG 1 cut(s) 36
HpyAV CCTTC 1 cut(s) 305
HpyCH4III ACNGT 1 cut(s) 288
HpyCH4IV ACGT 1 cut(s) 34
HpyCH4V TGCA 2 cut(s) 299, 343
HpyF10VI GCNNNNNNNGC 1 cut(s) 354
HpyF3I CTNAG 2 cut(s) 12, 276
HpySE526I ACGT 1 cut(s) 34
Hsp92I GRCGYC 1 cut(s) 34
Kpn2I TCCGGA 2 cut(s) 129, 164
Kzo9I GATC 2 cut(s) 196, 263
LguI GCTCTTC 1 cut(s) 202
Lsp1109I GCAGC 3 cut(s) 15, 55, 259
LweI GCATC 1 cut(s) 232
MaeII ACGT 1 cut(s) 34
MaeIII GTNAC 1 cut(s) 191
MalI GATC 2 cut(s) 198, 265
MbiI CCGCTC 1 cut(s) 212
MboI GATC 2 cut(s) 196, 263
MboII GAAGA 2 cut(s) 180, 219
MluCI AATT 2 cut(s) 160, 291
MlyI GAGTC 2 cut(s) 96, 368
MmeI TCCRAC 1 cut(s) 103
MnlI CCTC 6 cut(s) 7, 17, 109, 164, 277, 296
MroI TCCGGA 2 cut(s) 129, 164
MspI CCGG 6 cut(s) 99, 106, 130, 150, 165, 364
MspR9I CCNGG 3 cut(s) 143, 151, 364
MvaI CCWGG 1 cut(s) 143
MvnI CGCG 1 cut(s) 49
MwoI GCNNNNNNNGC 1 cut(s) 354
NciI CCSGG 2 cut(s) 151, 364
NdeII GATC 2 cut(s) 196, 263
NmuCI GTSAC 1 cut(s) 191
PciSI GCTCTTC 1 cut(s) 202
PfoI TCCNGGA 2 cut(s) 149, 362
PkrI GCNGC 4 cut(s) 5, 45, 48, 274
Ple19I CGATCG 1 cut(s) 199
PleI GAGTC 2 cut(s) 96, 367
PpsI GAGTC 2 cut(s) 96, 367
Psp6I CCWGG 1 cut(s) 141
PspGI CCWGG 1 cut(s) 141
PspPI GGNCC 1 cut(s) 139
PvuI CGATCG 1 cut(s) 199
RsaI GTAC 2 cut(s) 336, 390
RsaNI GTAC 2 cut(s) 335, 389
SapI GCTCTTC 1 cut(s) 202
SatI GCNGC 4 cut(s) 4, 44, 47, 273
Sau3AI GATC 2 cut(s) 196, 263
Sau96I GGNCC 1 cut(s) 139
ScaI AGTACT 1 cut(s) 336
SchI GAGTC 2 cut(s) 96, 368
ScrFI CCNGG 3 cut(s) 143, 151, 364
SetI ASST 8 cut(s) 37, 74, 120, 141, 147, 193, 307, 400
SfaNI GCATC 1 cut(s) 232
SinI GGWCC 1 cut(s) 139
SmlI CTYRAG 1 cut(s) 246
SmoI CTYRAG 1 cut(s) 246
Sse9I AATT 2 cut(s) 160, 291
SsiI CCGC 3 cut(s) 41, 47, 212
StyD4I CCNGG 3 cut(s) 141, 149, 362
TaaI ACNGT 1 cut(s) 288
TaiI ACGT 1 cut(s) 37
TaqI TCGA 2 cut(s) 31, 199
TasI AATT 2 cut(s) 160, 291
TatI WGTACW 1 cut(s) 334
TauI GCSGC 1 cut(s) 49
TseFI GTSAC 1 cut(s) 191
TseI GCWGC 3 cut(s) 3, 43, 272
Tsp45I GTSAC 1 cut(s) 191
VpaK11BI GGWCC 1 cut(s) 139
XagI CCTNNNNNAGG 2 cut(s) 11, 300
XapI RAATTY 1 cut(s) 160
ZraI GACGTC 1 cut(s) 35
ZrmI AGTACT 1 cut(s) 336
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.