Rw7G021550

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0010290
Physical Location & Seq
Forward (+)
25011932 .. 25013617
1686 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G021550.1

Sequence Viewer

Length: 732 bp
ATGAAATCATCTGCTCTGATCCGAACCGGTTCCATTGGTCTCTCCGGCTCTCCCGCCGTCTCCGGCTCTCCGAGAGTCTCCCTCTCTCGAACCGACTCGCTCTCCGGCGCCGAGAAGAGCACCGTCGCAGCCTCTCTGAGGGTTTGTCTCCACTTCGACGTCAACCGCAGCCGCGACAAGAACACTATTCGGCGAGCTGTATCCGAGAGCAACATTCTAAGATCAGACTCCGGTAAAGAGAGGTATATCCCGTCCGGAAGCAGGTCCAGGTCTCCGGGAAGTGGAATTCCGGAAGAGGAGATGGGAAGCAAAAAGGTGACGATCGAAAGAGAAGAGCGGAGAAAGATGGGAGAGTATTATCAGGCGATGCTCAAGTCTAATCCGAGTGATCCTCTGCTGCTCAGAAACTACGGTCAATTCCTGCACGAGGTGGAGAAGGATATGGTGCGAGCCGAGGAGTACTATTGCAGAGCCATATTGGCGAGTCCCGGAGACGGCGAGTTGTTGTCGCTGTACGGGAAGCTGATATGGGAGAGTCAGAGGGATAAGGATCGGGCCAATTCTTACTTTAACCAGGCTGTTTCGGCTTCACCCAACAATTGCATGGTTTTGGGATCCTTTGCTAGCTTCATGTGGGAAGTAGAGGAAGATGAAGAAAACGATGAAGAAATCAACAGTAACAGTACAACTGCTCAAGTCGCATCAGCAGCTCAGCTTGTTCCAGCATTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

243

Amino Acids

26.82

Weight (kDa)

5.64

Isoelectric Point (pI)

60.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_TmcB PF25474 115 - 158 6.3e-07 TmcB, TPR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 162
Acc36I ACCTGC 1 cut(s) 252
AccB1I GGYRCC 1 cut(s) 107
AccBSI CCGCTC 1 cut(s) 337
AccII CGCG 1 cut(s) 174
AccIII TCCGGA 2 cut(s) 254, 289
AciI CCGC 4 cut(s) 54, 166, 172, 337
AclWI GGATC 5 cut(s) 13, 383, 558, 609, 622
AcsI RAATTY 1 cut(s) 285
AcyI GRCGYC 2 cut(s) 108, 159
AdeI CACNNNGTG 1 cut(s) 430
AfaI GTAC 3 cut(s) 461, 515, 685
AfiI CCNNNNNNNGG 5 cut(s) 138, 261, 281, 427, 494
AgeI ACCGGT 1 cut(s) 26
AjnI CCWGG 2 cut(s) 266, 573
AluBI AGCT 5 cut(s) 197, 523, 627, 710, 715
AluI AGCT 5 cut(s) 197, 523, 627, 710, 715
Alw21I GWGCWC 1 cut(s) 122
Alw26I GTCTC 6 cut(s) 44, 64, 82, 152, 276, 486
AlwI GGATC 5 cut(s) 13, 383, 558, 609, 622
Aor13HI TCCGGA 2 cut(s) 254, 289
AoxI GGCC 1 cut(s) 555
ApeKI GCWGC 4 cut(s) 128, 168, 397, 707
ApoI RAATTY 1 cut(s) 285
AsiGI ACCGGT 1 cut(s) 26
Asp700I GAANNNNTTC 1 cut(s) 28
AspLEI GCGC 1 cut(s) 110
AspS9I GGNCC 2 cut(s) 264, 555
AsuC2I CCSGG 2 cut(s) 276, 489
AsuHPI GGTGA 2 cut(s) 328, 582
AsuNHI GCTAGC 1 cut(s) 623
AvaII GGWCC 1 cut(s) 264
BamHI GGATCC 1 cut(s) 614
BanI GGYRCC 1 cut(s) 107
BauI CACGAG 1 cut(s) 425
Bbv12I GWGCWC 1 cut(s) 122
BbvI GCAGC 4 cut(s) 140, 180, 384, 719
BccI CCATC 2 cut(s) 295, 340
BceAI ACGGC 2 cut(s) 41, 511
BciT130I CCWGG 2 cut(s) 268, 575
BciVI GTATCC 1 cut(s) 211
BcnI CCSGG 2 cut(s) 276, 489
BcoDI GTCTC 6 cut(s) 44, 64, 82, 152, 276, 486
BfaI CTAG 1 cut(s) 624
BfoI RGCGCY 1 cut(s) 111
BfuAI ACCTGC 1 cut(s) 252
BfuI GTATCC 1 cut(s) 211
BglI GCCNNNNNGGC 1 cut(s) 479
BisI GCNGC 5 cut(s) 129, 169, 172, 398, 708
BlpI GCTNAGC 1 cut(s) 711
BlsI GCNGC 5 cut(s) 130, 170, 173, 399, 709
BmcAI AGTACT 1 cut(s) 461
Bme1390I CCNGG 4 cut(s) 268, 276, 489, 575
Bme18I GGWCC 1 cut(s) 264
BmgT120I GGNCC 2 cut(s) 264, 555
BmiI GGNNCC 3 cut(s) 31, 109, 616
BmrFI CCNGG 4 cut(s) 268, 276, 489, 575
BmsI GCATC 2 cut(s) 357, 710
BmtI GCTAGC 1 cut(s) 627
BplI GAGNNNNNCTC 4 cut(s) 66, 98, 376, 408
Bpu1102I GCTNAGC 1 cut(s) 711
BpuEI CTTGAG 2 cut(s) 356, 678
BpuMI CCSGG 2 cut(s) 276, 489
BsaBI GATNNNNATC 1 cut(s) 549
BsaHI GRCGYC 2 cut(s) 108, 159
BsaI GGTCTC 2 cut(s) 44, 276
BsaJI CCNNGG 1 cut(s) 453
BsaWI WCCGGW 4 cut(s) 26, 230, 254, 289
BsaXI ACNNNNNCTCC 2 cut(s) 86, 116
Bsc4I CCNNNNNNNGG 5 cut(s) 138, 261, 281, 427, 494
Bse118I RCCGGY 1 cut(s) 26
Bse8I GATNNNNATC 1 cut(s) 549
BseAI TCCGGA 2 cut(s) 254, 289
BseBI CCWGG 2 cut(s) 268, 575
BseDI CCNNGG 1 cut(s) 453
BseJI GATNNNNATC 1 cut(s) 549
BseLI CCNNNNNNNGG 5 cut(s) 138, 261, 281, 427, 494
BseMII CTCAG 3 cut(s) 128, 415, 725
BseRI GAGGAG 2 cut(s) 311, 470
BseXI GCAGC 4 cut(s) 140, 180, 384, 719
BsgI GTGCAG 1 cut(s) 407
Bsh1236I CGCG 1 cut(s) 174
Bsh1285I CGRYCG 1 cut(s) 324
BshFI GGCC 1 cut(s) 557
BshNI GGYRCC 1 cut(s) 107
BshTI ACCGGT 1 cut(s) 26
BsiEI CGRYCG 1 cut(s) 324
BsiHKAI GWGCWC 1 cut(s) 122
BsiSI CCGG 9 cut(s) 27, 45, 63, 105, 231, 255, 275, 290, 489
BslFI GGGAC 1 cut(s) 471
BslI CCNNNNNNNGG 5 cut(s) 138, 261, 281, 427, 494
BsmAI GTCTC 6 cut(s) 44, 64, 82, 152, 276, 486
BsmBI CGTCTC 2 cut(s) 64, 486
BsmFI GGGAC 1 cut(s) 471
BsnI GGCC 1 cut(s) 557
Bso31I GGTCTC 2 cut(s) 44, 276
Bsp1286I GDGCHC 1 cut(s) 122
Bsp13I TCCGGA 2 cut(s) 254, 289
Bsp143I GATC 6 cut(s) 18, 221, 321, 388, 550, 614
Bsp1720I GCTNAGC 1 cut(s) 711
BspACI CCGC 4 cut(s) 54, 166, 172, 337
BspANI GGCC 1 cut(s) 557
BspCNI CTCAG 3 cut(s) 129, 414, 724
BspEI TCCGGA 2 cut(s) 254, 289
BspFNI CGCG 1 cut(s) 174
BspLI GGNNCC 3 cut(s) 31, 109, 616
BspMI ACCTGC 1 cut(s) 252
BspOI GCTAGC 1 cut(s) 627
BspPI GGATC 5 cut(s) 13, 383, 558, 609, 622
BspQI GCTCTTC 2 cut(s) 110, 327
BspT107I GGYRCC 1 cut(s) 107
BspTNI GGTCTC 2 cut(s) 44, 276
BsrBI CCGCTC 1 cut(s) 337
BsrFI RCCGGY 1 cut(s) 26
BssAI RCCGGY 1 cut(s) 26
BssECI CCNNGG 1 cut(s) 453
BssMI GATC 6 cut(s) 18, 221, 321, 388, 550, 614
BssNI GRCGYC 2 cut(s) 108, 159
BssSI CACGAG 1 cut(s) 425
Bst2BI CACGAG 1 cut(s) 425
Bst2UI CCWGG 2 cut(s) 268, 575
Bst4CI ACNGT 4 cut(s) 124, 413, 677, 683
Bst6I CTCTTC 3 cut(s) 110, 288, 327
BstACI GRCGYC 2 cut(s) 108, 159
BstC8I GCNNGC 3 cut(s) 195, 450, 625
BstDEI CTNAG 4 cut(s) 137, 218, 401, 711
BstENI CCTNNNNNAGG 2 cut(s) 136, 425
BstFNI CGCG 1 cut(s) 174
BstH2I RGCGCY 1 cut(s) 111
BstHHI GCGC 1 cut(s) 110
BstKTI GATC 6 cut(s) 21, 224, 324, 391, 553, 617
BstMAI GTCTC 6 cut(s) 44, 64, 82, 152, 276, 486
BstMBI GATC 6 cut(s) 18, 221, 321, 388, 550, 614
BstMCI CGRYCG 1 cut(s) 324
BstMWI GCNNNNNNNGC 4 cut(s) 479, 584, 698, 707
BstNI CCWGG 2 cut(s) 268, 575
BstSCI CCNGG 4 cut(s) 266, 274, 487, 573
BstUI CGCG 1 cut(s) 174
BstV1I GCAGC 4 cut(s) 140, 180, 384, 719
BstX2I RGATCY 1 cut(s) 614
BstYI RGATCY 1 cut(s) 614
BsuI GTATCC 1 cut(s) 211
BsuRI GGCC 1 cut(s) 557
BtgZI GCGATG 1 cut(s) 380
BveI ACCTGC 1 cut(s) 252
Cac8I GCNNGC 3 cut(s) 195, 450, 625
CfoI GCGC 1 cut(s) 110
Cfr10I RCCGGY 1 cut(s) 26
Cfr13I GGNCC 2 cut(s) 264, 555
Csp6I GTAC 3 cut(s) 460, 514, 684
CspAI ACCGGT 1 cut(s) 26
CviAII CATG 2 cut(s) 604, 631
CviQI GTAC 3 cut(s) 460, 514, 684
DdeI CTNAG 4 cut(s) 137, 218, 401, 711
DinI GGCGCC 1 cut(s) 109
DpnI GATC 6 cut(s) 20, 223, 323, 390, 552, 616
DpnII GATC 6 cut(s) 18, 221, 321, 388, 550, 614
DraIII CACNNNGTG 1 cut(s) 430
Eam1104I CTCTTC 3 cut(s) 110, 288, 327
EarI CTCTTC 3 cut(s) 110, 288, 327
Eco31I GGTCTC 2 cut(s) 44, 276
Eco47I GGWCC 1 cut(s) 264
EcoNI CCTNNNNNAGG 2 cut(s) 136, 425
EcoRI GAATTC 1 cut(s) 285
EcoRII CCWGG 2 cut(s) 266, 573
EgeI GGCGCC 1 cut(s) 109
EheI GGCGCC 1 cut(s) 109
Esp3I CGTCTC 2 cut(s) 64, 486
FaeI CATG 2 cut(s) 607, 634
FaiI YATR 6 cut(s) 246, 443, 476, 529, 605, 632
FaqI GGGAC 1 cut(s) 471
FatI CATG 2 cut(s) 603, 630
FauI CCCGC 1 cut(s) 61
Fnu4HI GCNGC 5 cut(s) 129, 169, 172, 398, 708
Fsp4HI GCNGC 5 cut(s) 129, 169, 172, 398, 708
FspBI CTAG 1 cut(s) 624
GlaI GCGC 1 cut(s) 109
GluI GCNGC 5 cut(s) 129, 169, 172, 398, 708
HaeII RGCGCY 1 cut(s) 111
HaeIII GGCC 1 cut(s) 557
HapII CCGG 9 cut(s) 27, 45, 63, 105, 231, 255, 275, 290, 489
HhaI GCGC 1 cut(s) 110
Hin1I GRCGYC 2 cut(s) 108, 159
Hin1II CATG 2 cut(s) 607, 634
Hin6I GCGC 1 cut(s) 108
HinP1I GCGC 1 cut(s) 108
HincII GTYRAC 1 cut(s) 163
HindII GTYRAC 1 cut(s) 163
HinfI GANTC 5 cut(s) 75, 95, 227, 484, 535
HpaII CCGG 9 cut(s) 27, 45, 63, 105, 231, 255, 275, 290, 489
HphI GGTGA 2 cut(s) 328, 582
Hpy166II GTNNAC 1 cut(s) 163
Hpy188I TCNGA 9 cut(s) 18, 23, 72, 138, 205, 226, 384, 404, 540
Hpy188III TCNNGA 3 cut(s) 87, 255, 290
Hpy8I GTNNAC 1 cut(s) 163
Hpy99I CGWCG 2 cut(s) 128, 161
HpyAV CCTTC 1 cut(s) 430
HpyCH4III ACNGT 4 cut(s) 124, 413, 677, 683
HpyCH4IV ACGT 1 cut(s) 159
HpyCH4V TGCA 3 cut(s) 424, 468, 603
HpyF10VI GCNNNNNNNGC 4 cut(s) 479, 584, 698, 707
HpyF3I CTNAG 4 cut(s) 137, 218, 401, 711
HpySE526I ACGT 1 cut(s) 159
Hsp92I GRCGYC 2 cut(s) 108, 159
Hsp92II CATG 2 cut(s) 607, 634
HspAI GCGC 1 cut(s) 108
KasI GGCGCC 1 cut(s) 107
Kpn2I TCCGGA 2 cut(s) 254, 289
Kzo9I GATC 6 cut(s) 18, 221, 321, 388, 550, 614
LguI GCTCTTC 2 cut(s) 110, 327
Lsp1109I GCAGC 4 cut(s) 140, 180, 384, 719
LweI GCATC 2 cut(s) 357, 710
MaeI CTAG 1 cut(s) 624
MaeII ACGT 1 cut(s) 159
MaeIII GTNAC 2 cut(s) 316, 677
MalI GATC 6 cut(s) 20, 223, 323, 390, 552, 616
MbiI CCGCTC 1 cut(s) 337
MboI GATC 6 cut(s) 18, 221, 321, 388, 550, 614
MboII GAAGA 6 cut(s) 127, 305, 344, 659, 665, 677
MfeI CAATTG 1 cut(s) 598
MflI RGATCY 1 cut(s) 614
MhlI GDGCHC 1 cut(s) 122
MluCI AATT 4 cut(s) 285, 416, 559, 598
Mly113I GGCGCC 1 cut(s) 108
MlyI GAGTC 5 cut(s) 84, 89, 221, 493, 544
MroI TCCGGA 2 cut(s) 254, 289
MroXI GAANNNNTTC 1 cut(s) 28
MseI TTAA 1 cut(s) 570
MspI CCGG 9 cut(s) 27, 45, 63, 105, 231, 255, 275, 290, 489
MspR9I CCNGG 4 cut(s) 268, 276, 489, 575
MunI CAATTG 1 cut(s) 598
MvaI CCWGG 2 cut(s) 268, 575
MvnI CGCG 1 cut(s) 174
MwoI GCNNNNNNNGC 4 cut(s) 479, 584, 698, 707
NarI GGCGCC 1 cut(s) 108
NciI CCSGG 2 cut(s) 276, 489
NdeII GATC 6 cut(s) 18, 221, 321, 388, 550, 614
NheI GCTAGC 1 cut(s) 623
NlaIII CATG 2 cut(s) 607, 634
NlaIV GGNNCC 3 cut(s) 31, 109, 616
NmeAIII GCCGAG 2 cut(s) 136, 478
NmuCI GTSAC 1 cut(s) 316
PciSI GCTCTTC 2 cut(s) 110, 327
PdmI GAANNNNTTC 1 cut(s) 28
PfoI TCCNGGA 2 cut(s) 274, 487
PinAI ACCGGT 1 cut(s) 26
PkrI GCNGC 5 cut(s) 130, 170, 173, 399, 709
Ple19I CGATCG 1 cut(s) 324
PleI GAGTC 5 cut(s) 83, 89, 221, 492, 543
PluTI GGCGCC 1 cut(s) 111
PpsI GAGTC 5 cut(s) 83, 89, 221, 492, 543
Psp6I CCWGG 2 cut(s) 266, 573
PspGI CCWGG 2 cut(s) 266, 573
PspN4I GGNNCC 3 cut(s) 31, 109, 616
PspPI GGNCC 2 cut(s) 264, 555
PsuI RGATCY 1 cut(s) 614
PvuI CGATCG 1 cut(s) 324
RsaI GTAC 3 cut(s) 461, 515, 685
RsaNI GTAC 3 cut(s) 460, 514, 684
SapI GCTCTTC 2 cut(s) 110, 327
SaqAI TTAA 1 cut(s) 570
SatI GCNGC 5 cut(s) 129, 169, 172, 398, 708
Sau3AI GATC 6 cut(s) 18, 221, 321, 388, 550, 614
Sau96I GGNCC 2 cut(s) 264, 555
ScaI AGTACT 1 cut(s) 461
SchI GAGTC 5 cut(s) 84, 89, 221, 493, 544
ScrFI CCNGG 4 cut(s) 268, 276, 489, 575
SduI GDGCHC 1 cut(s) 122
SfaNI GCATC 2 cut(s) 357, 710
SfoI GGCGCC 1 cut(s) 109
SinI GGWCC 1 cut(s) 264
SmlI CTYRAG 2 cut(s) 371, 693
SmoI CTYRAG 2 cut(s) 371, 693
Sse9I AATT 4 cut(s) 285, 416, 559, 598
SsiI CCGC 4 cut(s) 54, 166, 172, 337
SspDI GGCGCC 1 cut(s) 107
SspMI CTAG 1 cut(s) 624
StyD4I CCNGG 4 cut(s) 266, 274, 487, 573
TaaI ACNGT 4 cut(s) 124, 413, 677, 683
TaiI ACGT 1 cut(s) 162
TaqI TCGA 3 cut(s) 88, 156, 324
TasI AATT 4 cut(s) 285, 416, 559, 598
TatI WGTACW 2 cut(s) 459, 683
TauI GCSGC 1 cut(s) 174
Tru1I TTAA 1 cut(s) 570
Tru9I TTAA 1 cut(s) 570
TseFI GTSAC 1 cut(s) 316
TseI GCWGC 4 cut(s) 128, 168, 397, 707
Tsp45I GTSAC 1 cut(s) 316
TspDTI ATGAA 4 cut(s) 17, 619, 666, 678
VpaK11BI GGWCC 1 cut(s) 264
XagI CCTNNNNNAGG 2 cut(s) 136, 425
XapI RAATTY 1 cut(s) 285
XcmI CCANNNNNNNNNTGG 1 cut(s) 601
XmnI GAANNNNTTC 1 cut(s) 28
XspI CTAG 1 cut(s) 624
ZraI GACGTC 1 cut(s) 160
ZrmI AGTACT 1 cut(s) 461
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.