Rroxscaffold_3G00248530

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0010290
Physical Location & Seq
Reverse (-)
41853354 .. 41855470
2117 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00248530.1

Sequence Viewer

Length: 726 bp
ATGAAATCATCTGCTCTGATCCGAACCGGTTCCATTGGTCTCTCCGGCTCTCCCGCCGTCTCCGGCTCTCCGAGAGTCTTCCTCTCTCGAACCGACTCGCTCTCCGGCGCCGAGAAGAGCACCGTCGCAGCCTCTCTGAGGGTTTGTCTCCACTTCGACGTCAACCGCAGCCGCGACAAGAACACTATCCGGCGAGCTGTATCCGAGAGCAACATTCTACGATCGGACTCCGGTAAAGCGAGGTATATCCCGTCCGGAAGCAGGTCGCCGGGAAGTGGAATTCCGGAAGAGGAGATGGGAAGCAAAAAGGTGACGATCGAAAGAGAAGAGCGGAGAAAGATGGGAGAGTATTATCAGGAGATGCTCAAGTCTAATCCGAGTGATCCTCTGTTGCTCAGAAACTACGGTCAATTCCTGCACGAGGTGGAGAAGGATATGGTGCGAGCTGAGGAGTGCTATTGCAGAGCCATATTGGCGAGTCCCGGAGACGGCGAGTTGTTGTCGCTGTACGGGAAGCTGATATGGGAGAGTCAGAGGGATAAGGATCGGGCCAATTCTTACTTTAACCAGGCTCTTTCGGCTTCACCCAACAATTGCATGGTTTTGGGATCCTTTGCGAGCTTCATGTGGGAAGTAGAGGAAGATGAAGAAGACGATGAAGAAAACAACAGTAACAGTACAACTGCTCAAGTCTCATCAGCAGCTCAGCTTGTTCCAGCATTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

241

Amino Acids

26.61

Weight (kDa)

5.32

Isoelectric Point (pI)

60.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_TmcB PF25474 113 - 156 1.4e-06 TmcB, TPR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 162
Acc36I ACCTGC 1 cut(s) 252
AccB1I GGYRCC 1 cut(s) 107
AccBSI CCGCTC 1 cut(s) 331
AccII CGCG 1 cut(s) 174
AccIII TCCGGA 2 cut(s) 254, 283
AciI CCGC 4 cut(s) 54, 166, 172, 331
AclWI GGATC 5 cut(s) 13, 377, 552, 603, 616
AcsI RAATTY 1 cut(s) 279
AcyI GRCGYC 2 cut(s) 108, 159
AdeI CACNNNGTG 1 cut(s) 424
AfaI GTAC 2 cut(s) 509, 679
AfiI CCNNNNNNNGG 5 cut(s) 138, 261, 275, 421, 488
AgeI ACCGGT 1 cut(s) 26
AjnI CCWGG 1 cut(s) 567
AluBI AGCT 6 cut(s) 197, 446, 517, 621, 704, 709
AluI AGCT 6 cut(s) 197, 446, 517, 621, 704, 709
Alw21I GWGCWC 1 cut(s) 122
Alw26I GTCTC 5 cut(s) 44, 64, 152, 480, 697
AlwI GGATC 5 cut(s) 13, 377, 552, 603, 616
Aor13HI TCCGGA 2 cut(s) 254, 283
AoxI GGCC 1 cut(s) 549
ApeKI GCWGC 3 cut(s) 128, 168, 701
ApoI RAATTY 1 cut(s) 279
AsiGI ACCGGT 1 cut(s) 26
Asp700I GAANNNNTTC 1 cut(s) 28
AspLEI GCGC 1 cut(s) 110
AspS9I GGNCC 1 cut(s) 549
AsuC2I CCSGG 2 cut(s) 270, 483
AsuHPI GGTGA 2 cut(s) 322, 576
BamHI GGATCC 1 cut(s) 608
BanI GGYRCC 1 cut(s) 107
BauI CACGAG 1 cut(s) 419
BbsI GAAGAC 2 cut(s) 70, 657
Bbv12I GWGCWC 1 cut(s) 122
BbvCI CCTCAGC 1 cut(s) 447
BbvI GCAGC 3 cut(s) 140, 180, 713
BccI CCATC 2 cut(s) 289, 334
BceAI ACGGC 2 cut(s) 41, 505
BciT130I CCWGG 1 cut(s) 569
BciVI GTATCC 1 cut(s) 211
BcnI CCSGG 2 cut(s) 270, 483
BcoDI GTCTC 5 cut(s) 44, 64, 152, 480, 697
BfoI RGCGCY 1 cut(s) 111
BfuAI ACCTGC 1 cut(s) 252
BfuI GTATCC 1 cut(s) 211
BglI GCCNNNNNGGC 1 cut(s) 473
BisI GCNGC 4 cut(s) 129, 169, 172, 702
BlpI GCTNAGC 1 cut(s) 705
BlsI GCNGC 4 cut(s) 130, 170, 173, 703
Bme1390I CCNGG 3 cut(s) 270, 483, 569
BmgT120I GGNCC 1 cut(s) 549
BmiI GGNNCC 3 cut(s) 31, 109, 610
BmrFI CCNGG 3 cut(s) 270, 483, 569
BmsI GCATC 1 cut(s) 351
BpiI GAAGAC 2 cut(s) 70, 657
BplI GAGNNNNNCTC 4 cut(s) 66, 98, 370, 402
Bpu10I CCTNAGC 1 cut(s) 447
Bpu1102I GCTNAGC 1 cut(s) 705
BpuEI CTTGAG 2 cut(s) 350, 672
BpuMI CCSGG 2 cut(s) 270, 483
BsaBI GATNNNNATC 1 cut(s) 543
BsaHI GRCGYC 2 cut(s) 108, 159
BsaI GGTCTC 1 cut(s) 44
BsaWI WCCGGW 4 cut(s) 26, 230, 254, 283
BsaXI ACNNNNNCTCC 2 cut(s) 86, 116
Bsc4I CCNNNNNNNGG 5 cut(s) 138, 261, 275, 421, 488
Bse118I RCCGGY 1 cut(s) 26
Bse8I GATNNNNATC 1 cut(s) 543
BseAI TCCGGA 2 cut(s) 254, 283
BseBI CCWGG 1 cut(s) 569
BseJI GATNNNNATC 1 cut(s) 543
BseLI CCNNNNNNNGG 5 cut(s) 138, 261, 275, 421, 488
BseMII CTCAG 4 cut(s) 128, 409, 438, 719
BseRI GAGGAG 2 cut(s) 305, 464
BseXI GCAGC 3 cut(s) 140, 180, 713
BsgI GTGCAG 1 cut(s) 401
Bsh1236I CGCG 1 cut(s) 174
Bsh1285I CGRYCG 2 cut(s) 224, 318
BshFI GGCC 1 cut(s) 551
BshNI GGYRCC 1 cut(s) 107
BshTI ACCGGT 1 cut(s) 26
BsiEI CGRYCG 2 cut(s) 224, 318
BsiHKAI GWGCWC 1 cut(s) 122
BslFI GGGAC 1 cut(s) 465
BslI CCNNNNNNNGG 5 cut(s) 138, 261, 275, 421, 488
BsmAI GTCTC 5 cut(s) 44, 64, 152, 480, 697
BsmBI CGTCTC 2 cut(s) 64, 480
BsmFI GGGAC 1 cut(s) 465
BsnI GGCC 1 cut(s) 551
Bso31I GGTCTC 1 cut(s) 44
Bsp1286I GDGCHC 1 cut(s) 122
Bsp13I TCCGGA 2 cut(s) 254, 283
Bsp143I GATC 6 cut(s) 18, 221, 315, 382, 544, 608
Bsp1720I GCTNAGC 1 cut(s) 705
BspACI CCGC 4 cut(s) 54, 166, 172, 331
BspANI GGCC 1 cut(s) 551
BspCNI CTCAG 4 cut(s) 129, 408, 439, 718
BspEI TCCGGA 2 cut(s) 254, 283
BspFNI CGCG 1 cut(s) 174
BspLI GGNNCC 3 cut(s) 31, 109, 610
BspMI ACCTGC 1 cut(s) 252
BspPI GGATC 5 cut(s) 13, 377, 552, 603, 616
BspQI GCTCTTC 2 cut(s) 110, 321
BspT107I GGYRCC 1 cut(s) 107
BspTNI GGTCTC 1 cut(s) 44
BsrBI CCGCTC 1 cut(s) 331
BsrFI RCCGGY 1 cut(s) 26
BssAI RCCGGY 1 cut(s) 26
BssMI GATC 6 cut(s) 18, 221, 315, 382, 544, 608
BssNI GRCGYC 2 cut(s) 108, 159
BssSI CACGAG 1 cut(s) 419
Bst2BI CACGAG 1 cut(s) 419
Bst2UI CCWGG 1 cut(s) 569
Bst4CI ACNGT 4 cut(s) 124, 407, 671, 677
Bst6I CTCTTC 3 cut(s) 110, 282, 321
BstACI GRCGYC 2 cut(s) 108, 159
BstC8I GCNNGC 3 cut(s) 195, 444, 619
BstDEI CTNAG 4 cut(s) 137, 395, 447, 705
BstENI CCTNNNNNAGG 2 cut(s) 136, 419
BstFNI CGCG 1 cut(s) 174
BstH2I RGCGCY 1 cut(s) 111
BstHHI GCGC 1 cut(s) 110
BstKTI GATC 6 cut(s) 21, 224, 318, 385, 547, 611
BstMAI GTCTC 5 cut(s) 44, 64, 152, 480, 697
BstMBI GATC 6 cut(s) 18, 221, 315, 382, 544, 608
BstMCI CGRYCG 2 cut(s) 224, 318
BstMWI GCNNNNNNNGC 2 cut(s) 473, 578
BstNI CCWGG 1 cut(s) 569
BstSCI CCNGG 3 cut(s) 268, 481, 567
BstUI CGCG 1 cut(s) 174
BstV1I GCAGC 3 cut(s) 140, 180, 713
BstV2I GAAGAC 2 cut(s) 70, 657
BstX2I RGATCY 1 cut(s) 608
BstYI RGATCY 1 cut(s) 608
BsuI GTATCC 1 cut(s) 211
BsuRI GGCC 1 cut(s) 551
BveI ACCTGC 1 cut(s) 252
Cac8I GCNNGC 3 cut(s) 195, 444, 619
CfoI GCGC 1 cut(s) 110
Cfr10I RCCGGY 1 cut(s) 26
Cfr13I GGNCC 1 cut(s) 549
Csp6I GTAC 2 cut(s) 508, 678
CspAI ACCGGT 1 cut(s) 26
CviAII CATG 2 cut(s) 598, 625
CviQI GTAC 2 cut(s) 508, 678
DdeI CTNAG 4 cut(s) 137, 395, 447, 705
DinI GGCGCC 1 cut(s) 109
DpnI GATC 6 cut(s) 20, 223, 317, 384, 546, 610
DpnII GATC 6 cut(s) 18, 221, 315, 382, 544, 608
DraIII CACNNNGTG 1 cut(s) 424
Eam1104I CTCTTC 3 cut(s) 110, 282, 321
EarI CTCTTC 3 cut(s) 110, 282, 321
Eco31I GGTCTC 1 cut(s) 44
EcoNI CCTNNNNNAGG 2 cut(s) 136, 419
EcoRI GAATTC 1 cut(s) 279
EcoRII CCWGG 1 cut(s) 567
EgeI GGCGCC 1 cut(s) 109
EheI GGCGCC 1 cut(s) 109
Esp3I CGTCTC 2 cut(s) 64, 480
FaeI CATG 2 cut(s) 601, 628
FaiI YATR 6 cut(s) 246, 437, 470, 523, 599, 626
FaqI GGGAC 1 cut(s) 465
FatI CATG 2 cut(s) 597, 624
FauI CCCGC 1 cut(s) 61
Fnu4HI GCNGC 4 cut(s) 129, 169, 172, 702
Fsp4HI GCNGC 4 cut(s) 129, 169, 172, 702
GlaI GCGC 1 cut(s) 109
GluI GCNGC 4 cut(s) 129, 169, 172, 702
HaeII RGCGCY 1 cut(s) 111
HaeIII GGCC 1 cut(s) 551
HhaI GCGC 1 cut(s) 110
Hin1I GRCGYC 2 cut(s) 108, 159
Hin1II CATG 2 cut(s) 601, 628
Hin6I GCGC 1 cut(s) 108
HinP1I GCGC 1 cut(s) 108
HincII GTYRAC 1 cut(s) 163
HindII GTYRAC 1 cut(s) 163
HinfI GANTC 5 cut(s) 75, 95, 227, 478, 529
HphI GGTGA 2 cut(s) 322, 576
Hpy166II GTNNAC 1 cut(s) 163
Hpy188I TCNGA 9 cut(s) 18, 23, 72, 138, 205, 226, 378, 398, 534
Hpy188III TCNNGA 4 cut(s) 87, 255, 284, 356
Hpy8I GTNNAC 1 cut(s) 163
Hpy99I CGWCG 2 cut(s) 128, 161
HpyAV CCTTC 1 cut(s) 424
HpyCH4III ACNGT 4 cut(s) 124, 407, 671, 677
HpyCH4IV ACGT 1 cut(s) 159
HpyCH4V TGCA 3 cut(s) 418, 462, 597
HpyF10VI GCNNNNNNNGC 2 cut(s) 473, 578
HpyF3I CTNAG 4 cut(s) 137, 395, 447, 705
HpySE526I ACGT 1 cut(s) 159
Hsp92I GRCGYC 2 cut(s) 108, 159
Hsp92II CATG 2 cut(s) 601, 628
HspAI GCGC 1 cut(s) 108
KasI GGCGCC 1 cut(s) 107
Kpn2I TCCGGA 2 cut(s) 254, 283
Kzo9I GATC 6 cut(s) 18, 221, 315, 382, 544, 608
LguI GCTCTTC 2 cut(s) 110, 321
Lsp1109I GCAGC 3 cut(s) 140, 180, 713
LweI GCATC 1 cut(s) 351
MaeII ACGT 1 cut(s) 159
MaeIII GTNAC 2 cut(s) 310, 671
MalI GATC 6 cut(s) 20, 223, 317, 384, 546, 610
MbiI CCGCTC 1 cut(s) 331
MboI GATC 6 cut(s) 18, 221, 315, 382, 544, 608
MboII GAAGA 8 cut(s) 70, 127, 299, 338, 653, 659, 662, 671
MfeI CAATTG 1 cut(s) 592
MflI RGATCY 1 cut(s) 608
MhlI GDGCHC 1 cut(s) 122
MluCI AATT 4 cut(s) 279, 410, 553, 592
Mly113I GGCGCC 1 cut(s) 108
MlyI GAGTC 5 cut(s) 84, 89, 221, 487, 538
MroI TCCGGA 2 cut(s) 254, 283
MroXI GAANNNNTTC 1 cut(s) 28
MseI TTAA 1 cut(s) 564
MspR9I CCNGG 3 cut(s) 270, 483, 569
MunI CAATTG 1 cut(s) 592
MvaI CCWGG 1 cut(s) 569
MvnI CGCG 1 cut(s) 174
MwoI GCNNNNNNNGC 2 cut(s) 473, 578
NarI GGCGCC 1 cut(s) 108
NciI CCSGG 2 cut(s) 270, 483
NdeII GATC 6 cut(s) 18, 221, 315, 382, 544, 608
NlaIII CATG 2 cut(s) 601, 628
NlaIV GGNNCC 3 cut(s) 31, 109, 610
NmeAIII GCCGAG 1 cut(s) 136
NmuCI GTSAC 1 cut(s) 310
PciSI GCTCTTC 2 cut(s) 110, 321
PdmI GAANNNNTTC 1 cut(s) 28
PfoI TCCNGGA 1 cut(s) 481
PinAI ACCGGT 1 cut(s) 26
PkrI GCNGC 4 cut(s) 130, 170, 173, 703
Ple19I CGATCG 2 cut(s) 224, 318
PleI GAGTC 5 cut(s) 83, 89, 221, 486, 537
PluTI GGCGCC 1 cut(s) 111
PpsI GAGTC 5 cut(s) 83, 89, 221, 486, 537
Psp6I CCWGG 1 cut(s) 567
PspGI CCWGG 1 cut(s) 567
PspN4I GGNNCC 3 cut(s) 31, 109, 610
PspPI GGNCC 1 cut(s) 549
PsuI RGATCY 1 cut(s) 608
PvuI CGATCG 2 cut(s) 224, 318
RsaI GTAC 2 cut(s) 509, 679
RsaNI GTAC 2 cut(s) 508, 678
SapI GCTCTTC 2 cut(s) 110, 321
SaqAI TTAA 1 cut(s) 564
SatI GCNGC 4 cut(s) 129, 169, 172, 702
Sau3AI GATC 6 cut(s) 18, 221, 315, 382, 544, 608
Sau96I GGNCC 1 cut(s) 549
SchI GAGTC 5 cut(s) 84, 89, 221, 487, 538
ScrFI CCNGG 3 cut(s) 270, 483, 569
SduI GDGCHC 1 cut(s) 122
SfaNI GCATC 1 cut(s) 351
SfoI GGCGCC 1 cut(s) 109
SmlI CTYRAG 2 cut(s) 365, 687
SmoI CTYRAG 2 cut(s) 365, 687
Sse9I AATT 4 cut(s) 279, 410, 553, 592
SsiI CCGC 4 cut(s) 54, 166, 172, 331
SspDI GGCGCC 1 cut(s) 107
StyD4I CCNGG 3 cut(s) 268, 481, 567
TaaI ACNGT 4 cut(s) 124, 407, 671, 677
TaiI ACGT 1 cut(s) 162
TaqI TCGA 3 cut(s) 88, 156, 318
TasI AATT 4 cut(s) 279, 410, 553, 592
TatI WGTACW 1 cut(s) 677
TauI GCSGC 1 cut(s) 174
Tru1I TTAA 1 cut(s) 564
Tru9I TTAA 1 cut(s) 564
TseFI GTSAC 1 cut(s) 310
TseI GCWGC 3 cut(s) 128, 168, 701
Tsp45I GTSAC 1 cut(s) 310
TspDTI ATGAA 4 cut(s) 17, 613, 660, 672
XagI CCTNNNNNAGG 2 cut(s) 136, 419
XapI RAATTY 1 cut(s) 279
XcmI CCANNNNNNNNNTGG 1 cut(s) 595
XmnI GAANNNNTTC 1 cut(s) 28
ZraI GACGTC 1 cut(s) 160
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.