FvH4_5g23100

component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0008374
Physical Location & Seq
Reverse (-)
14448144 .. 14452565
4422 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g23100.t1

Sequence Viewer

Length: 678 bp
ATGGAGGACTGGGAAGAAGATGTGCAAGTTCCATCTCTCCTTTCTAAGGAGCCAGTGGTAAGAAGTAACTGGGATGATGAAGATGCTGATGACAATGAGGTGAAGGATTCATGGGAGGATGATGATGAACCTGCTCCTCCGGCACCTGCACCAAAAGCTCCTGCTGAAAAGGCACCCAGGAAACCTGCAGCAAAGGCTGCAGAAAAGAAAGGAAAAGCTGTTGAAGTAGAAAAGGAAGAGTCACTAGATCCCCTGGCTGAGAAACTTCGCCAACAAAGACTGGTGGAAGAAGCAGATTATAAGGCCACTAAAGAACTTTTTTCTACTAGAGGTGAAGAGAAAAACATTGACAATTTCATTCCCAAATCGGAAAGTGACTTTCTGGAATATGCAGAACTCATTTCACATAAACTTCGTCCATTTGAGAAAAGTTTTCATTATATCGGTCTACTTAAGGCAGTTATGAGATTGTCAATGACCTCCTTGAAAGGAGCAGATGCAAAAGATGTTGCATCTTCAATCACGGCAATTGCAAATGAGAAGATAAAAGCTGAGAAGGAAGCCAATGCTGGTAAAAAGAAGACAGGTGCCAAGAAAAAGCAGCTTCATGTTGATAAGCCGGATGATGATATAGCTGTTGATGGTTATGATCCCCTCGATGACTATGACTTTATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
eIF3_subunit

Protein Analysis

226

Amino Acids

25.23

Weight (kDa)

4.76

Isoelectric Point (pI)

40.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
eIF3_subunit PF08597 3 - 225 4.4e-53 Translation initiation factor eIF3 subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 300
AarI CACCTGC 1 cut(s) 154
Acc36I ACCTGC 3 cut(s) 139, 154, 193
AccB1I GGYRCC 3 cut(s) 142, 172, 587
AccI GTMKAC 1 cut(s) 448
AclWI GGATC 2 cut(s) 242, 644
AfiI CCNNNNNNNGG 1 cut(s) 46
AflII CTTAAG 1 cut(s) 452
AgsI TTSAA 3 cut(s) 224, 487, 519
AjnI CCWGG 2 cut(s) 176, 252
AluBI AGCT 5 cut(s) 158, 218, 551, 604, 635
AluI AGCT 5 cut(s) 158, 218, 551, 604, 635
AlwI GGATC 2 cut(s) 242, 644
AoxI GGCC 1 cut(s) 303
ApeKI GCWGC 3 cut(s) 188, 197, 601
AsuHPI GGTGA 2 cut(s) 112, 344
BanI GGYRCC 3 cut(s) 142, 172, 587
BbsI GAAGAC 1 cut(s) 587
BbvI GCAGC 3 cut(s) 184, 200, 613
BccI CCATC 2 cut(s) 40, 635
BceAI ACGGC 1 cut(s) 540
BciT130I CCWGG 2 cut(s) 178, 254
BfaI CTAG 2 cut(s) 245, 327
BfmI CTRYAG 2 cut(s) 186, 198
BfrI CTTAAG 1 cut(s) 452
BfuAI ACCTGC 3 cut(s) 139, 154, 193
BisI GCNGC 3 cut(s) 189, 198, 602
BlsI GCNGC 3 cut(s) 190, 199, 603
Bme1390I CCNGG 2 cut(s) 178, 254
BmiI GGNNCC 4 cut(s) 51, 144, 174, 589
BmrFI CCNGG 2 cut(s) 178, 254
BmrI ACTGGG 2 cut(s) 19, 79
BmsI GCATC 3 cut(s) 73, 487, 521
BmuI ACTGGG 2 cut(s) 19, 79
BpiI GAAGAC 1 cut(s) 587
BsaJI CCNNGG 2 cut(s) 176, 252
Bsc4I CCNNNNNNNGG 1 cut(s) 46
Bse1I ACTGG 4 cut(s) 14, 53, 74, 285
BseBI CCWGG 2 cut(s) 178, 254
BseDI CCNNGG 2 cut(s) 176, 252
BseGI GGATG 3 cut(s) 79, 124, 628
BseLI CCNNNNNNNGG 1 cut(s) 46
BseMII CTCAG 2 cut(s) 249, 543
BseNI ACTGG 4 cut(s) 14, 53, 74, 285
BseRI GAGGAG 1 cut(s) 126
BseXI GCAGC 3 cut(s) 184, 200, 613
BsgI GTGCAG 1 cut(s) 132
BshFI GGCC 1 cut(s) 305
BshNI GGYRCC 3 cut(s) 142, 172, 587
BsiSI CCGG 2 cut(s) 140, 620
BslI CCNNNNNNNGG 1 cut(s) 46
BsnI GGCC 1 cut(s) 305
Bsp143I GATC 2 cut(s) 247, 649
BspANI GGCC 1 cut(s) 305
BspCNI CTCAG 2 cut(s) 250, 544
BspLI GGNNCC 4 cut(s) 51, 144, 174, 589
BspMAI CTGCAG 2 cut(s) 190, 202
BspMI ACCTGC 3 cut(s) 139, 154, 193
BspPI GGATC 2 cut(s) 242, 644
BspT107I GGYRCC 3 cut(s) 142, 172, 587
BspTI CTTAAG 1 cut(s) 452
BsrI ACTGG 4 cut(s) 14, 53, 74, 285
BssECI CCNNGG 2 cut(s) 176, 252
BssMI GATC 2 cut(s) 247, 649
Bst2UI CCWGG 2 cut(s) 178, 254
Bst6I CTCTTC 2 cut(s) 231, 330
BstAFI CTTAAG 1 cut(s) 452
BstAPI GCANNNNNTGC 1 cut(s) 197
BstDEI CTNAG 3 cut(s) 45, 258, 552
BstENI CCTNNNNNAGG 1 cut(s) 44
BstF5I GGATG 3 cut(s) 79, 124, 628
BstKTI GATC 2 cut(s) 250, 652
BstMBI GATC 2 cut(s) 247, 649
BstMWI GCNNNNNNNGC 5 cut(s) 140, 155, 170, 194, 197
BstNI CCWGG 2 cut(s) 178, 254
BstSCI CCNGG 2 cut(s) 176, 252
BstSFI CTRYAG 2 cut(s) 186, 198
BstV1I GCAGC 3 cut(s) 184, 200, 613
BstV2I GAAGAC 1 cut(s) 587
BstX2I RGATCY 1 cut(s) 247
BstYI RGATCY 1 cut(s) 247
BsuRI GGCC 1 cut(s) 305
BtsCI GGATG 3 cut(s) 79, 124, 628
BtsIMutI CAGTG 1 cut(s) 60
BveI ACCTGC 3 cut(s) 139, 154, 193
CviAII CATG 2 cut(s) 111, 608
DdeI CTNAG 3 cut(s) 45, 258, 552
DpnI GATC 2 cut(s) 249, 651
DpnII GATC 2 cut(s) 247, 649
Eam1104I CTCTTC 2 cut(s) 231, 330
EarI CTCTTC 2 cut(s) 231, 330
EcoNI CCTNNNNNAGG 1 cut(s) 44
EcoRII CCWGG 2 cut(s) 176, 252
FaeI CATG 2 cut(s) 114, 611
FatI CATG 2 cut(s) 110, 607
FblI GTMKAC 1 cut(s) 448
Fnu4HI GCNGC 3 cut(s) 189, 198, 602
FokI GGATG 3 cut(s) 86, 131, 635
Fsp4HI GCNGC 3 cut(s) 189, 198, 602
FspBI CTAG 2 cut(s) 245, 327
GluI GCNGC 3 cut(s) 189, 198, 602
HaeIII GGCC 1 cut(s) 305
HapII CCGG 2 cut(s) 140, 620
Hin1II CATG 2 cut(s) 114, 611
HinfI GANTC 2 cut(s) 107, 239
HpaII CCGG 2 cut(s) 140, 620
HphI GGTGA 2 cut(s) 112, 344
Hpy166II GTNNAC 1 cut(s) 449
Hpy188I TCNGA 1 cut(s) 370
Hpy188III TCNNGA 1 cut(s) 383
Hpy8I GTNNAC 1 cut(s) 449
HpyAV CCTTC 2 cut(s) 97, 550
HpyCH4V TGCA 8 cut(s) 25, 149, 188, 200, 392, 500, 512, 533
HpyF10VI GCNNNNNNNGC 5 cut(s) 140, 155, 170, 194, 197
HpyF3I CTNAG 3 cut(s) 45, 258, 552
Hsp92II CATG 2 cut(s) 114, 611
Kzo9I GATC 2 cut(s) 247, 649
LmnI GCTCC 4 cut(s) 49, 139, 163, 491
Lsp1109I GCAGC 3 cut(s) 184, 200, 613
LweI GCATC 3 cut(s) 73, 487, 521
MaeI CTAG 2 cut(s) 245, 327
MaeIII GTNAC 3 cut(s) 65, 240, 374
MalI GATC 2 cut(s) 249, 651
MboI GATC 2 cut(s) 247, 649
MboII GAAGA 9 cut(s) 26, 29, 92, 248, 299, 347, 507, 553, 592
MfeI CAATTG 1 cut(s) 528
MflI RGATCY 1 cut(s) 247
MluCI AATT 2 cut(s) 352, 528
MlyI GAGTC 1 cut(s) 248
MnlI CCTC 6 cut(s) 91, 109, 147, 323, 490, 665
MseI TTAA 1 cut(s) 453
MspCI CTTAAG 1 cut(s) 452
MspI CCGG 2 cut(s) 140, 620
MspR9I CCNGG 2 cut(s) 178, 254
MunI CAATTG 1 cut(s) 528
MvaI CCWGG 2 cut(s) 178, 254
MwoI GCNNNNNNNGC 5 cut(s) 140, 155, 170, 194, 197
NdeII GATC 2 cut(s) 247, 649
NlaIII CATG 2 cut(s) 114, 611
NlaIV GGNNCC 4 cut(s) 51, 144, 174, 589
NmuCI GTSAC 2 cut(s) 240, 374
PaqCI CACCTGC 1 cut(s) 154
PfeI GAWTC 1 cut(s) 107
PkrI GCNGC 3 cut(s) 190, 199, 603
PleI GAGTC 1 cut(s) 247
PpsI GAGTC 1 cut(s) 247
PsiI TTATAA 1 cut(s) 300
Psp6I CCWGG 2 cut(s) 176, 252
PspGI CCWGG 2 cut(s) 176, 252
PspN4I GGNNCC 4 cut(s) 51, 144, 174, 589
PstI CTGCAG 2 cut(s) 190, 202
PsuI RGATCY 1 cut(s) 247
SaqAI TTAA 1 cut(s) 453
SatI GCNGC 3 cut(s) 189, 198, 602
Sau3AI GATC 2 cut(s) 247, 649
SchI GAGTC 1 cut(s) 248
ScrFI CCNGG 2 cut(s) 178, 254
SfaNI GCATC 3 cut(s) 73, 487, 521
SfcI CTRYAG 2 cut(s) 186, 198
SmlI CTYRAG 1 cut(s) 452
SmoI CTYRAG 1 cut(s) 452
Sse9I AATT 2 cut(s) 352, 528
SspMI CTAG 2 cut(s) 245, 327
StyD4I CCNGG 2 cut(s) 176, 252
TaqI TCGA 1 cut(s) 657
TaqII GACCGA 1 cut(s) 434
TasI AATT 2 cut(s) 352, 528
TfiI GAWTC 1 cut(s) 107
Tru1I TTAA 1 cut(s) 453
Tru9I TTAA 1 cut(s) 453
TscAI CASTG 1 cut(s) 60
TseFI GTSAC 2 cut(s) 240, 374
TseI GCWGC 3 cut(s) 188, 197, 601
Tsp45I GTSAC 2 cut(s) 240, 374
TspDTI ATGAA 6 cut(s) 93, 99, 141, 346, 425, 596
TspRI CASTG 1 cut(s) 60
Vha464I CTTAAG 1 cut(s) 452
XagI CCTNNNNNAGG 1 cut(s) 44
XmiI GTMKAC 1 cut(s) 448
XspI CTAG 2 cut(s) 245, 327
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.