Rw7G022410

component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0008374
Physical Location & Seq
Reverse (-)
26329079 .. 26331590
2512 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G022410.1

Sequence Viewer

Length: 672 bp
ATGGAGGACTGGGAAGATGCGAGAATTCCATCCCTCCTTTCTAAGGAGCCTGCAGTAAAAGGTAACTGGGATGATGAAGATGCGGATGAGAATGACGTGAAGGACTCATGGGAGGATGATGATGAACCTGCTCCTCCGGAACCTGCACCAAAGGCTCCTGCTGAAAAGGCTCCCAAGAAACCTGCAACAAAGGCTGCAGAAAAGAAAGGAAAAGCTGTTGAAGTACAAAAGGAAGAGCCACTAGATCCCCTGGCTGAGAAACTTCGCCAACAAAGACTGGTGGAAGAAGCAGATTATAAGGCCACTAAAGAACTGTTTTCTACTAGAGATGAGAAGAACATTGATAATTTCATTCCCAAATCCGAAAGTGACTTCCTGGAATATGCCGAACTTATTTCACATAAACTTCGTCCATTTGAGAAAAGTTTTCATTATATCGGTCTACTTAAGGCAGTAATGAGACTGTCAATGACTTCTTTGAAAGGAGCTGATGCAAAAGATGTTGCTTCTTCAATTACGGCAATTGCAAATGAGAAGATAAAAGCTGAGAAGGAAGCCAATGCTGGTAAAAAGAAGACAGGTGCCAAGAAAAAGCAGCTTCATGTTGATAAGCCGGATGATGATATAGCTGTTGATGGCTATGATCCCCTTGATGACTATGACTTTATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
eIF3_subunit

Protein Analysis

223

Amino Acids

25.03

Weight (kDa)

4.83

Isoelectric Point (pI)

35.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
eIF3_subunit PF08597 2 - 223 3e-53 Translation initiation factor eIF3 subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 297
Acc36I ACCTGC 3 cut(s) 136, 151, 190
AccB1I GGYRCC 1 cut(s) 581
AccI GTMKAC 1 cut(s) 442
AccIII TCCGGA 1 cut(s) 136
AciI CCGC 1 cut(s) 83
AclWI GGATC 2 cut(s) 239, 638
AcsI RAATTY 1 cut(s) 24
AfaI GTAC 1 cut(s) 225
AfiI CCNNNNNNNGG 1 cut(s) 43
AflII CTTAAG 1 cut(s) 446
AgsI TTSAA 3 cut(s) 221, 481, 513
AjiI CACGTC 1 cut(s) 97
AjnI CCWGG 2 cut(s) 249, 375
AluBI AGCT 5 cut(s) 215, 488, 545, 598, 629
AluI AGCT 5 cut(s) 215, 488, 545, 598, 629
Alw26I GTCTC 1 cut(s) 454
AlwI GGATC 2 cut(s) 239, 638
Aor13HI TCCGGA 1 cut(s) 136
AoxI GGCC 1 cut(s) 300
ApeKI GCWGC 2 cut(s) 194, 595
ApoI RAATTY 1 cut(s) 24
BanI GGYRCC 1 cut(s) 581
BbsI GAAGAC 1 cut(s) 581
BbvI GCAGC 2 cut(s) 181, 607
BccI CCATC 2 cut(s) 37, 629
BceAI ACGGC 1 cut(s) 534
BciT130I CCWGG 2 cut(s) 251, 377
BcoDI GTCTC 1 cut(s) 454
BfaI CTAG 2 cut(s) 242, 324
BfmI CTRYAG 2 cut(s) 51, 195
BfrI CTTAAG 1 cut(s) 446
BfuAI ACCTGC 3 cut(s) 136, 151, 190
BisI GCNGC 2 cut(s) 195, 596
BlsI GCNGC 2 cut(s) 196, 597
Bme1390I CCNGG 2 cut(s) 251, 377
BmgBI CACGTC 1 cut(s) 97
BmiI GGNNCC 5 cut(s) 48, 141, 156, 171, 583
BmrFI CCNGG 2 cut(s) 251, 377
BmrI ACTGGG 2 cut(s) 19, 76
BmsI GCATC 3 cut(s) 7, 70, 481
BmuI ACTGGG 2 cut(s) 19, 76
BpiI GAAGAC 1 cut(s) 581
BsaJI CCNNGG 1 cut(s) 249
BsaWI WCCGGW 1 cut(s) 136
Bsc4I CCNNNNNNNGG 1 cut(s) 43
Bse1I ACTGG 3 cut(s) 14, 71, 282
BseAI TCCGGA 1 cut(s) 136
BseBI CCWGG 2 cut(s) 251, 377
BseDI CCNNGG 1 cut(s) 249
BseGI GGATG 5 cut(s) 29, 76, 91, 121, 622
BseLI CCNNNNNNNGG 1 cut(s) 43
BseMII CTCAG 2 cut(s) 246, 537
BseNI ACTGG 3 cut(s) 14, 71, 282
BseRI GAGGAG 1 cut(s) 123
BseXI GCAGC 2 cut(s) 181, 607
BsgI GTGCAG 1 cut(s) 129
BshFI GGCC 1 cut(s) 302
BshNI GGYRCC 1 cut(s) 581
BsiSI CCGG 2 cut(s) 137, 614
BslI CCNNNNNNNGG 1 cut(s) 43
BsmAI GTCTC 1 cut(s) 454
BsnI GGCC 1 cut(s) 302
Bsp13I TCCGGA 1 cut(s) 136
Bsp143I GATC 2 cut(s) 244, 643
BspACI CCGC 1 cut(s) 83
BspANI GGCC 1 cut(s) 302
BspCNI CTCAG 2 cut(s) 247, 538
BspEI TCCGGA 1 cut(s) 136
BspLI GGNNCC 5 cut(s) 48, 141, 156, 171, 583
BspMAI CTGCAG 2 cut(s) 55, 199
BspMI ACCTGC 3 cut(s) 136, 151, 190
BspPI GGATC 2 cut(s) 239, 638
BspQI GCTCTTC 1 cut(s) 228
BspT107I GGYRCC 1 cut(s) 581
BspTI CTTAAG 1 cut(s) 446
BsrI ACTGG 3 cut(s) 14, 71, 282
BssECI CCNNGG 1 cut(s) 249
BssMI GATC 2 cut(s) 244, 643
Bst2UI CCWGG 2 cut(s) 251, 377
Bst4CI ACNGT 2 cut(s) 315, 465
Bst6I CTCTTC 1 cut(s) 228
BstAFI CTTAAG 1 cut(s) 446
BstC8I GCNNGC 1 cut(s) 51
BstDEI CTNAG 3 cut(s) 42, 255, 546
BstENI CCTNNNNNAGG 1 cut(s) 41
BstF5I GGATG 5 cut(s) 29, 76, 91, 121, 622
BstKTI GATC 2 cut(s) 247, 646
BstMAI GTCTC 1 cut(s) 454
BstMBI GATC 2 cut(s) 244, 643
BstMWI GCNNNNNNNGC 3 cut(s) 152, 167, 191
BstNI CCWGG 2 cut(s) 251, 377
BstSCI CCNGG 2 cut(s) 249, 375
BstSFI CTRYAG 2 cut(s) 51, 195
BstV1I GCAGC 2 cut(s) 181, 607
BstV2I GAAGAC 1 cut(s) 581
BstX2I RGATCY 1 cut(s) 244
BstYI RGATCY 1 cut(s) 244
BsuRI GGCC 1 cut(s) 302
BtrI CACGTC 1 cut(s) 97
BtsCI GGATG 5 cut(s) 29, 76, 91, 121, 622
BveI ACCTGC 3 cut(s) 136, 151, 190
Cac8I GCNNGC 1 cut(s) 51
Csp6I GTAC 1 cut(s) 224
CviAII CATG 2 cut(s) 108, 602
CviQI GTAC 1 cut(s) 224
DdeI CTNAG 3 cut(s) 42, 255, 546
DpnI GATC 2 cut(s) 246, 645
DpnII GATC 2 cut(s) 244, 643
Eam1104I CTCTTC 1 cut(s) 228
EarI CTCTTC 1 cut(s) 228
EcoNI CCTNNNNNAGG 1 cut(s) 41
EcoRI GAATTC 1 cut(s) 24
EcoRII CCWGG 2 cut(s) 249, 375
FaeI CATG 2 cut(s) 111, 605
FatI CATG 2 cut(s) 107, 601
FblI GTMKAC 1 cut(s) 442
Fnu4HI GCNGC 2 cut(s) 195, 596
FokI GGATG 5 cut(s) 16, 83, 98, 128, 629
Fsp4HI GCNGC 2 cut(s) 195, 596
FspBI CTAG 2 cut(s) 242, 324
GluI GCNGC 2 cut(s) 195, 596
HaeIII GGCC 1 cut(s) 302
HapII CCGG 2 cut(s) 137, 614
Hin1II CATG 2 cut(s) 111, 605
HinfI GANTC 1 cut(s) 104
HpaII CCGG 2 cut(s) 137, 614
Hpy166II GTNNAC 1 cut(s) 443
Hpy188I TCNGA 1 cut(s) 364
Hpy188III TCNNGA 1 cut(s) 137
Hpy8I GTNNAC 1 cut(s) 443
HpyAV CCTTC 2 cut(s) 94, 544
HpyCH4III ACNGT 2 cut(s) 315, 465
HpyCH4IV ACGT 1 cut(s) 96
HpyCH4V TGCA 6 cut(s) 53, 146, 185, 197, 494, 527
HpyF10VI GCNNNNNNNGC 3 cut(s) 152, 167, 191
HpyF3I CTNAG 3 cut(s) 42, 255, 546
HpySE526I ACGT 1 cut(s) 96
Hsp92II CATG 2 cut(s) 111, 605
Kpn2I TCCGGA 1 cut(s) 136
Kzo9I GATC 2 cut(s) 244, 643
LguI GCTCTTC 1 cut(s) 228
LmnI GCTCC 5 cut(s) 46, 136, 160, 175, 485
Lsp1109I GCAGC 2 cut(s) 181, 607
LweI GCATC 3 cut(s) 7, 70, 481
MaeI CTAG 2 cut(s) 242, 324
MaeII ACGT 1 cut(s) 96
MaeIII GTNAC 2 cut(s) 62, 368
MalI GATC 2 cut(s) 246, 645
MboI GATC 2 cut(s) 244, 643
MboII GAAGA 8 cut(s) 26, 89, 245, 296, 346, 501, 547, 586
MfeI CAATTG 1 cut(s) 522
MflI RGATCY 1 cut(s) 244
MluCI AATT 4 cut(s) 24, 346, 513, 522
MlyI GAGTC 1 cut(s) 98
MnlI CCTC 3 cut(s) 44, 106, 144
MroI TCCGGA 1 cut(s) 136
MseI TTAA 1 cut(s) 447
MspCI CTTAAG 1 cut(s) 446
MspI CCGG 2 cut(s) 137, 614
MspR9I CCNGG 2 cut(s) 251, 377
MunI CAATTG 1 cut(s) 522
MvaI CCWGG 2 cut(s) 251, 377
MwoI GCNNNNNNNGC 3 cut(s) 152, 167, 191
NdeII GATC 2 cut(s) 244, 643
NlaIII CATG 2 cut(s) 111, 605
NlaIV GGNNCC 5 cut(s) 48, 141, 156, 171, 583
NmuCI GTSAC 1 cut(s) 368
PciSI GCTCTTC 1 cut(s) 228
PfoI TCCNGGA 1 cut(s) 375
PkrI GCNGC 2 cut(s) 196, 597
PleI GAGTC 1 cut(s) 98
PpsI GAGTC 1 cut(s) 98
PsiI TTATAA 1 cut(s) 297
Psp6I CCWGG 2 cut(s) 249, 375
PspGI CCWGG 2 cut(s) 249, 375
PspN4I GGNNCC 5 cut(s) 48, 141, 156, 171, 583
PstI CTGCAG 2 cut(s) 55, 199
PsuI RGATCY 1 cut(s) 244
RsaI GTAC 1 cut(s) 225
RsaNI GTAC 1 cut(s) 224
SapI GCTCTTC 1 cut(s) 228
SaqAI TTAA 1 cut(s) 447
SatI GCNGC 2 cut(s) 195, 596
Sau3AI GATC 2 cut(s) 244, 643
SchI GAGTC 1 cut(s) 98
ScrFI CCNGG 2 cut(s) 251, 377
SfaNI GCATC 3 cut(s) 7, 70, 481
SfcI CTRYAG 2 cut(s) 51, 195
SmlI CTYRAG 1 cut(s) 446
SmoI CTYRAG 1 cut(s) 446
Sse9I AATT 4 cut(s) 24, 346, 513, 522
SsiI CCGC 1 cut(s) 83
SspMI CTAG 2 cut(s) 242, 324
StyD4I CCNGG 2 cut(s) 249, 375
TaaI ACNGT 2 cut(s) 315, 465
TaiI ACGT 1 cut(s) 99
TaqII GACCGA 1 cut(s) 428
TasI AATT 4 cut(s) 24, 346, 513, 522
TatI WGTACW 1 cut(s) 223
Tru1I TTAA 1 cut(s) 447
Tru9I TTAA 1 cut(s) 447
TseFI GTSAC 1 cut(s) 368
TseI GCWGC 2 cut(s) 194, 595
Tsp45I GTSAC 1 cut(s) 368
TspDTI ATGAA 5 cut(s) 90, 138, 340, 419, 590
Vha464I CTTAAG 1 cut(s) 446
XagI CCTNNNNNAGG 1 cut(s) 41
XapI RAATTY 1 cut(s) 24
XmiI GTMKAC 1 cut(s) 442
XspI CTAG 2 cut(s) 242, 324
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.