pycom06g02170

component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0008374
Physical Location & Seq
Forward (+)
2611173 .. 2615403
4231 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g02170.2

Sequence Viewer

Length: 684 bp
ATGGAGGACTGGGAAGAAGAGAAAGTTCCAGCTCTCCTTGCTAAGGACAAACCGATGCATAAATGGGATGACGAAGATGTTGATGAAAATGATATTACGGAGTCATGGGAGGATTTAGATGAACCTGCTCCTCTGGAACCTGCAGCAAAGCCTGTTGCTGAGAAGGCACCTAAGAAACCTGCAACAAAACCTGCTGCAAAAGCTACAGAAAAGAAAGGAAAGATCGTTGAAGTAGAAAAGGAAGAGCCACTGGATCCTGTGGCTGAGAAACTTCGCCAACAAAGGCTGGTGGAAGAAGCAGATTATAAGTCCACTAAAGAGTTGTTTTCCAGTGGAGGCAATGAAAAAAACATTGACAATTTCATTCCGAAATCCGAAAATGACTTCCTGGAATATGCAGAACTTATTTCGCATAAACTTCGTCCATTTGAGAAAAGTTTCCATTATATTGGTCTACTCAAGGCAGTAATGAGACTGTCAATGACGTCGTTGAAAGGAGCAGATGCGAAAGAAGTTGCATCTTCCATTACGGCAATTGCAAATGAGAAGATAAAAGCAGAGAAGGAAGCAAATGCTGGCAAAAAGAAAACAGGTGCCAAGAAAAAGCAGCTAATAGTTGATAAGGCGGATGATGATATAGCTGTTGAGGGCTATGATCCACTCGATGATTATGATTTTATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
eIF3_subunit

Protein Analysis

228

Amino Acids

25.5

Weight (kDa)

4.87

Isoelectric Point (pI)

38.78

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 306
AatII GACGTC 1 cut(s) 488
Acc36I ACCTGC 4 cut(s) 133, 148, 187, 199
AccB1I GGYRCC 2 cut(s) 166, 593
AccI GTMKAC 1 cut(s) 454
AciI CCGC 1 cut(s) 626
AclWI GGATC 3 cut(s) 248, 261, 650
AcyI GRCGYC 1 cut(s) 485
AfiI CCNNNNNNNGG 1 cut(s) 43
AgsI TTSAA 2 cut(s) 230, 493
AjnI CCWGG 1 cut(s) 387
AluBI AGCT 4 cut(s) 32, 203, 610, 641
AluI AGCT 4 cut(s) 32, 203, 610, 641
Alw26I GTCTC 1 cut(s) 466
AlwI GGATC 3 cut(s) 248, 261, 650
ApeKI GCWGC 3 cut(s) 143, 194, 607
Asp700I GAANNNNTTC 1 cut(s) 437
BamHI GGATCC 1 cut(s) 253
BanI GGYRCC 2 cut(s) 166, 593
BbvI GCAGC 3 cut(s) 155, 181, 619
BceAI ACGGC 1 cut(s) 546
BcgI CGANNNNNNTGC 2 cut(s) 401, 435
BciT130I CCWGG 1 cut(s) 389
BcoDI GTCTC 1 cut(s) 466
BfmI CTRYAG 2 cut(s) 141, 204
BfuAI ACCTGC 4 cut(s) 133, 148, 187, 199
BisI GCNGC 3 cut(s) 144, 195, 608
BlsI GCNGC 3 cut(s) 145, 196, 609
Bme1390I CCNGG 1 cut(s) 389
BmiI GGNNCC 4 cut(s) 138, 168, 255, 595
BmrFI CCNGG 1 cut(s) 389
BmrI ACTGGG 1 cut(s) 19
BmsI GCATC 3 cut(s) 45, 493, 527
BmuI ACTGGG 1 cut(s) 19
Bpu10I CCTNAGC 1 cut(s) 42
BpuEI CTTGAG 1 cut(s) 443
BsaHI GRCGYC 1 cut(s) 485
Bsc4I CCNNNNNNNGG 1 cut(s) 43
Bse1I ACTGG 3 cut(s) 14, 255, 330
Bse3DI GCAATG 1 cut(s) 346
BseBI CCWGG 1 cut(s) 389
BseGI GGATG 2 cut(s) 73, 634
BseLI CCNNNNNNNGG 1 cut(s) 43
BseMI GCAATG 1 cut(s) 346
BseMII CTCAG 2 cut(s) 150, 255
BseNI ACTGG 3 cut(s) 14, 255, 330
BseRI GAGGAG 1 cut(s) 120
BseXI GCAGC 3 cut(s) 155, 181, 619
BshNI GGYRCC 2 cut(s) 166, 593
BslI CCNNNNNNNGG 1 cut(s) 43
BsmAI GTCTC 1 cut(s) 466
Bsp143I GATC 3 cut(s) 222, 253, 655
BspACI CCGC 1 cut(s) 626
BspCNI CTCAG 2 cut(s) 151, 256
BspLI GGNNCC 4 cut(s) 138, 168, 255, 595
BspMAI CTGCAG 1 cut(s) 145
BspMI ACCTGC 4 cut(s) 133, 148, 187, 199
BspPI GGATC 3 cut(s) 248, 261, 650
BspQI GCTCTTC 1 cut(s) 237
BspT107I GGYRCC 2 cut(s) 166, 593
BsrDI GCAATG 1 cut(s) 346
BsrI ACTGG 3 cut(s) 14, 255, 330
BssMI GATC 3 cut(s) 222, 253, 655
BssNI GRCGYC 1 cut(s) 485
Bst2UI CCWGG 1 cut(s) 389
Bst4CI ACNGT 1 cut(s) 477
Bst6I CTCTTC 2 cut(s) 12, 237
BstACI GRCGYC 1 cut(s) 485
BstC8I GCNNGC 1 cut(s) 577
BstDEI CTNAG 4 cut(s) 42, 159, 171, 264
BstENI CCTNNNNNAGG 1 cut(s) 41
BstF5I GGATG 2 cut(s) 73, 634
BstKTI GATC 3 cut(s) 225, 256, 658
BstMAI GTCTC 1 cut(s) 466
BstMBI GATC 3 cut(s) 222, 253, 655
BstMWI GCNNNNNNNGC 3 cut(s) 38, 164, 200
BstNI CCWGG 1 cut(s) 389
BstSCI CCNGG 1 cut(s) 387
BstSFI CTRYAG 2 cut(s) 141, 204
BstV1I GCAGC 3 cut(s) 155, 181, 619
BstX2I RGATCY 1 cut(s) 253
BstXI CCANNNNNNTGG 1 cut(s) 449
BstYI RGATCY 1 cut(s) 253
BtsCI GGATG 2 cut(s) 73, 634
BtsIMutI CAGTG 2 cut(s) 248, 337
BveI ACCTGC 4 cut(s) 133, 148, 187, 199
Cac8I GCNNGC 1 cut(s) 577
CviAII CATG 1 cut(s) 105
CviJI RGCY 9 cut(s) 32, 151, 203, 247, 263, 286, 610, 641, 651
CviKI_1 RGCY 9 cut(s) 32, 151, 203, 247, 263, 286, 610, 641, 651
DdeI CTNAG 4 cut(s) 42, 159, 171, 264
DpnI GATC 3 cut(s) 224, 255, 657
DpnII GATC 3 cut(s) 222, 253, 655
Eam1104I CTCTTC 2 cut(s) 12, 237
EarI CTCTTC 2 cut(s) 12, 237
EciI GGCGGA 1 cut(s) 641
EcoNI CCTNNNNNAGG 1 cut(s) 41
EcoRII CCWGG 1 cut(s) 387
EcoT22I ATGCAT 1 cut(s) 60
FaeI CATG 1 cut(s) 108
FatI CATG 1 cut(s) 104
FblI GTMKAC 1 cut(s) 454
Fnu4HI GCNGC 3 cut(s) 144, 195, 608
FokI GGATG 2 cut(s) 80, 641
Fsp4HI GCNGC 3 cut(s) 144, 195, 608
GluI GCNGC 3 cut(s) 144, 195, 608
Hin1I GRCGYC 1 cut(s) 485
Hin1II CATG 1 cut(s) 108
HinfI GANTC 1 cut(s) 101
Hpy166II GTNNAC 2 cut(s) 312, 455
Hpy188I TCNGA 2 cut(s) 369, 376
Hpy188III TCNNGA 1 cut(s) 134
Hpy8I GTNNAC 2 cut(s) 312, 455
Hpy99I CGWCG 1 cut(s) 490
HpyAV CCTTC 2 cut(s) 157, 556
HpyCH4III ACNGT 1 cut(s) 477
HpyCH4IV ACGT 1 cut(s) 485
HpyCH4V TGCA 7 cut(s) 58, 143, 182, 197, 398, 518, 539
HpyF10VI GCNNNNNNNGC 3 cut(s) 38, 164, 200
HpyF3I CTNAG 4 cut(s) 42, 159, 171, 264
HpySE526I ACGT 1 cut(s) 485
Hsp92I GRCGYC 1 cut(s) 485
Hsp92II CATG 1 cut(s) 108
Kzo9I GATC 3 cut(s) 222, 253, 655
LguI GCTCTTC 1 cut(s) 237
LmnI GCTCC 2 cut(s) 133, 497
Lsp1109I GCAGC 3 cut(s) 155, 181, 619
LweI GCATC 3 cut(s) 45, 493, 527
MaeII ACGT 1 cut(s) 485
MalI GATC 3 cut(s) 224, 255, 657
MboI GATC 3 cut(s) 222, 253, 655
MboII GAAGA 7 cut(s) 26, 29, 86, 254, 305, 513, 559
MfeI CAATTG 1 cut(s) 534
MflI RGATCY 1 cut(s) 253
MluCI AATT 2 cut(s) 358, 534
MlyI GAGTC 1 cut(s) 110
MnlI CCTC 4 cut(s) 103, 141, 329, 640
Mph1103I ATGCAT 1 cut(s) 60
MroXI GAANNNNTTC 1 cut(s) 437
MspR9I CCNGG 1 cut(s) 389
MunI CAATTG 1 cut(s) 534
MvaI CCWGG 1 cut(s) 389
MwoI GCNNNNNNNGC 3 cut(s) 38, 164, 200
NdeII GATC 3 cut(s) 222, 253, 655
NlaIII CATG 1 cut(s) 108
NlaIV GGNNCC 4 cut(s) 138, 168, 255, 595
NsiI ATGCAT 1 cut(s) 60
PciSI GCTCTTC 1 cut(s) 237
PdmI GAANNNNTTC 1 cut(s) 437
PfoI TCCNGGA 1 cut(s) 387
PkrI GCNGC 3 cut(s) 145, 196, 609
PleI GAGTC 1 cut(s) 109
PpsI GAGTC 1 cut(s) 109
PsiI TTATAA 1 cut(s) 306
Psp6I CCWGG 1 cut(s) 387
PspGI CCWGG 1 cut(s) 387
PspN4I GGNNCC 4 cut(s) 138, 168, 255, 595
PstI CTGCAG 1 cut(s) 145
PsuI RGATCY 1 cut(s) 253
SapI GCTCTTC 1 cut(s) 237
SatI GCNGC 3 cut(s) 144, 195, 608
Sau3AI GATC 3 cut(s) 222, 253, 655
SchI GAGTC 1 cut(s) 110
ScrFI CCNGG 1 cut(s) 389
SfaNI GCATC 3 cut(s) 45, 493, 527
SfcI CTRYAG 2 cut(s) 141, 204
SmlI CTYRAG 1 cut(s) 458
SmoI CTYRAG 1 cut(s) 458
Sse9I AATT 2 cut(s) 358, 534
SsiI CCGC 1 cut(s) 626
StyD4I CCNGG 1 cut(s) 387
TaaI ACNGT 1 cut(s) 477
TaiI ACGT 1 cut(s) 488
TaqI TCGA 1 cut(s) 663
TasI AATT 2 cut(s) 358, 534
TscAI CASTG 2 cut(s) 255, 337
TseI GCWGC 3 cut(s) 143, 194, 607
TspDTI ATGAA 4 cut(s) 99, 135, 352, 357
TspGWI ACGGA 1 cut(s) 113
TspRI CASTG 2 cut(s) 255, 337
XagI CCTNNNNNAGG 1 cut(s) 41
XmiI GTMKAC 1 cut(s) 454
XmnI GAANNNNTTC 1 cut(s) 437
ZraI GACGTC 1 cut(s) 486
Zsp2I ATGCAT 1 cut(s) 60
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.