Rh7AG262600

component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0008374
Physical Location & Seq
Reverse (-)
26942859 .. 26947354
4496 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG262600.1

Sequence Viewer

Length: 615 bp
ATGGAGGACTGGGAAGAAGATGCGAGAATTCCATCCCTCCTTTCTAAGGAGCCAGCAGTAAAAGGTAACTGGGATGATGAAGATGCGGATGAGAATGACGTGAAGGACTCATGGGAGGATGATGATGAACCTGCTCCTCCGGAACCTGCACCAAAGGCTCCTGCTGAAAAGGCTCCCAAGAAACCTGCAACAAAGGCTGCAGAAAAGAAAGGAAAAGCTGTTGAAGTACAAAAGGAAGAGCCACTAGATCCCCTGGCTGAGAAACTTCTCCAACAAAGACTGGTGGAAGAAGCAGATTATAAGGCCACTAAAGAACTGTTTTCTACTAGAGATGAGAAGAACATTGATAATTTCATTCCCAAATCCGAAAGTGACTTCCTGGAATATGCCGAACTTATTTCACATAAACTTCGTCCATTTGAGAAAAGTTTTCATTATATCGGTCTACTTAAGGCAGTAATGAGACTGTCAATGACTTCTTTGAAAGGAGCTGATGCAAAAGATGTTGCTTCTTCAATTACGGCAATTGCAAATGAGAAGATAAAAGCTGAGAAGGAAGCCAATGCTGGTAAAAAGAAGACAGGTGCGTATCTTTACGTACCTAATTTGCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
eIF3_subunit

Protein Analysis

204

Amino Acids

22.81

Weight (kDa)

4.9

Isoelectric Point (pI)

39.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
eIF3_subunit PF08597 2 - 196 5e-48 Translation initiation factor eIF3 subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 300
Acc36I ACCTGC 3 cut(s) 139, 154, 193
AccI GTMKAC 1 cut(s) 445
AccIII TCCGGA 1 cut(s) 139
AciI CCGC 1 cut(s) 86
AclWI GGATC 1 cut(s) 242
AcsI RAATTY 1 cut(s) 27
AfaI GTAC 2 cut(s) 228, 600
AfiI CCNNNNNNNGG 1 cut(s) 46
AflII CTTAAG 1 cut(s) 449
AgsI TTSAA 3 cut(s) 224, 484, 516
AjiI CACGTC 1 cut(s) 100
AjnI CCWGG 2 cut(s) 252, 378
AluBI AGCT 3 cut(s) 218, 491, 548
AluI AGCT 3 cut(s) 218, 491, 548
Alw26I GTCTC 1 cut(s) 457
AlwI GGATC 1 cut(s) 242
Aor13HI TCCGGA 1 cut(s) 139
AoxI GGCC 1 cut(s) 303
ApeKI GCWGC 1 cut(s) 197
ApoI RAATTY 1 cut(s) 27
BbsI GAAGAC 1 cut(s) 584
BbvI GCAGC 1 cut(s) 184
BccI CCATC 1 cut(s) 40
BceAI ACGGC 1 cut(s) 537
BciT130I CCWGG 2 cut(s) 254, 380
BcoDI GTCTC 1 cut(s) 457
BfaI CTAG 2 cut(s) 245, 327
BfmI CTRYAG 1 cut(s) 198
BfrI CTTAAG 1 cut(s) 449
BfuAI ACCTGC 3 cut(s) 139, 154, 193
BisI GCNGC 1 cut(s) 198
BlsI GCNGC 1 cut(s) 199
Bme1390I CCNGG 2 cut(s) 254, 380
BmgBI CACGTC 1 cut(s) 100
BmiI GGNNCC 4 cut(s) 51, 144, 159, 174
BmrFI CCNGG 2 cut(s) 254, 380
BmrI ACTGGG 2 cut(s) 19, 79
BmsI GCATC 3 cut(s) 10, 73, 484
BmuI ACTGGG 2 cut(s) 19, 79
BpiI GAAGAC 1 cut(s) 584
BsaAI YACGTR 1 cut(s) 598
BsaJI CCNNGG 1 cut(s) 252
BsaWI WCCGGW 1 cut(s) 139
Bsc4I CCNNNNNNNGG 1 cut(s) 46
Bse1I ACTGG 3 cut(s) 14, 74, 285
BseAI TCCGGA 1 cut(s) 139
BseBI CCWGG 2 cut(s) 254, 380
BseDI CCNNGG 1 cut(s) 252
BseGI GGATG 4 cut(s) 32, 79, 94, 124
BseLI CCNNNNNNNGG 1 cut(s) 46
BseMII CTCAG 2 cut(s) 249, 540
BseNI ACTGG 3 cut(s) 14, 74, 285
BseRI GAGGAG 1 cut(s) 126
BseXI GCAGC 1 cut(s) 184
BsgI GTGCAG 1 cut(s) 132
BshFI GGCC 1 cut(s) 305
BsiSI CCGG 1 cut(s) 140
BslI CCNNNNNNNGG 1 cut(s) 46
BsmAI GTCTC 1 cut(s) 457
BsnI GGCC 1 cut(s) 305
Bsp13I TCCGGA 1 cut(s) 139
Bsp143I GATC 1 cut(s) 247
BspACI CCGC 1 cut(s) 86
BspANI GGCC 1 cut(s) 305
BspCNI CTCAG 2 cut(s) 250, 541
BspEI TCCGGA 1 cut(s) 139
BspLI GGNNCC 4 cut(s) 51, 144, 159, 174
BspMAI CTGCAG 1 cut(s) 202
BspMI ACCTGC 3 cut(s) 139, 154, 193
BspPI GGATC 1 cut(s) 242
BspQI GCTCTTC 1 cut(s) 231
BspTI CTTAAG 1 cut(s) 449
BsrI ACTGG 3 cut(s) 14, 74, 285
BssECI CCNNGG 1 cut(s) 252
BssMI GATC 1 cut(s) 247
Bst2UI CCWGG 2 cut(s) 254, 380
Bst4CI ACNGT 2 cut(s) 318, 468
Bst6I CTCTTC 1 cut(s) 231
BstAFI CTTAAG 1 cut(s) 449
BstBAI YACGTR 1 cut(s) 598
BstC8I GCNNGC 1 cut(s) 54
BstDEI CTNAG 3 cut(s) 45, 258, 549
BstENI CCTNNNNNAGG 1 cut(s) 44
BstF5I GGATG 4 cut(s) 32, 79, 94, 124
BstKTI GATC 1 cut(s) 250
BstMAI GTCTC 1 cut(s) 457
BstMBI GATC 1 cut(s) 247
BstMWI GCNNNNNNNGC 3 cut(s) 155, 170, 194
BstNI CCWGG 2 cut(s) 254, 380
BstSCI CCNGG 2 cut(s) 252, 378
BstSFI CTRYAG 1 cut(s) 198
BstSNI TACGTA 1 cut(s) 598
BstV1I GCAGC 1 cut(s) 184
BstV2I GAAGAC 1 cut(s) 584
BstX2I RGATCY 1 cut(s) 247
BstYI RGATCY 1 cut(s) 247
BsuRI GGCC 1 cut(s) 305
BtrI CACGTC 1 cut(s) 100
BtsCI GGATG 4 cut(s) 32, 79, 94, 124
BveI ACCTGC 3 cut(s) 139, 154, 193
Cac8I GCNNGC 1 cut(s) 54
Csp6I GTAC 2 cut(s) 227, 599
CviAII CATG 1 cut(s) 111
CviQI GTAC 2 cut(s) 227, 599
DdeI CTNAG 3 cut(s) 45, 258, 549
DpnI GATC 1 cut(s) 249
DpnII GATC 1 cut(s) 247
Eam1104I CTCTTC 1 cut(s) 231
EarI CTCTTC 1 cut(s) 231
Eco105I TACGTA 1 cut(s) 598
EcoNI CCTNNNNNAGG 1 cut(s) 44
EcoRI GAATTC 1 cut(s) 27
EcoRII CCWGG 2 cut(s) 252, 378
FaeI CATG 1 cut(s) 114
FaiI YATR 5 cut(s) 112, 300, 387, 405, 438
FatI CATG 1 cut(s) 110
FblI GTMKAC 1 cut(s) 445
Fnu4HI GCNGC 1 cut(s) 198
FokI GGATG 4 cut(s) 19, 86, 101, 131
Fsp4HI GCNGC 1 cut(s) 198
FspBI CTAG 2 cut(s) 245, 327
GluI GCNGC 1 cut(s) 198
HaeIII GGCC 1 cut(s) 305
HapII CCGG 1 cut(s) 140
Hin1II CATG 1 cut(s) 114
HinfI GANTC 1 cut(s) 107
HpaII CCGG 1 cut(s) 140
Hpy166II GTNNAC 1 cut(s) 446
Hpy188I TCNGA 1 cut(s) 367
Hpy188III TCNNGA 1 cut(s) 140
Hpy8I GTNNAC 1 cut(s) 446
HpyAV CCTTC 2 cut(s) 97, 547
HpyCH4III ACNGT 2 cut(s) 318, 468
HpyCH4IV ACGT 2 cut(s) 99, 597
HpyCH4V TGCA 5 cut(s) 149, 188, 200, 497, 530
HpyF10VI GCNNNNNNNGC 3 cut(s) 155, 170, 194
HpyF3I CTNAG 3 cut(s) 45, 258, 549
HpySE526I ACGT 2 cut(s) 99, 597
Hsp92II CATG 1 cut(s) 114
Kpn2I TCCGGA 1 cut(s) 139
Kzo9I GATC 1 cut(s) 247
LguI GCTCTTC 1 cut(s) 231
LmnI GCTCC 5 cut(s) 49, 139, 163, 178, 488
Lsp1109I GCAGC 1 cut(s) 184
LweI GCATC 3 cut(s) 10, 73, 484
MaeI CTAG 2 cut(s) 245, 327
MaeII ACGT 2 cut(s) 99, 597
MaeIII GTNAC 2 cut(s) 65, 371
MalI GATC 1 cut(s) 249
MboI GATC 1 cut(s) 247
MboII GAAGA 9 cut(s) 26, 29, 92, 248, 299, 349, 504, 550, 589
MfeI CAATTG 1 cut(s) 525
MflI RGATCY 1 cut(s) 247
MluCI AATT 5 cut(s) 27, 349, 516, 525, 604
MlyI GAGTC 1 cut(s) 101
MmeI TCCRAC 1 cut(s) 295
MnlI CCTC 3 cut(s) 47, 109, 147
MroI TCCGGA 1 cut(s) 139
MseI TTAA 1 cut(s) 450
MspCI CTTAAG 1 cut(s) 449
MspI CCGG 1 cut(s) 140
MspR9I CCNGG 2 cut(s) 254, 380
MunI CAATTG 1 cut(s) 525
MvaI CCWGG 2 cut(s) 254, 380
MwoI GCNNNNNNNGC 3 cut(s) 155, 170, 194
NdeII GATC 1 cut(s) 247
NlaIII CATG 1 cut(s) 114
NlaIV GGNNCC 4 cut(s) 51, 144, 159, 174
NmuCI GTSAC 1 cut(s) 371
PciSI GCTCTTC 1 cut(s) 231
PfoI TCCNGGA 1 cut(s) 378
PkrI GCNGC 1 cut(s) 199
PleI GAGTC 1 cut(s) 101
PpsI GAGTC 1 cut(s) 101
Ppu21I YACGTR 1 cut(s) 598
PsiI TTATAA 1 cut(s) 300
Psp6I CCWGG 2 cut(s) 252, 378
PspGI CCWGG 2 cut(s) 252, 378
PspN4I GGNNCC 4 cut(s) 51, 144, 159, 174
PstI CTGCAG 1 cut(s) 202
PsuI RGATCY 1 cut(s) 247
RsaI GTAC 2 cut(s) 228, 600
RsaNI GTAC 2 cut(s) 227, 599
SapI GCTCTTC 1 cut(s) 231
SaqAI TTAA 1 cut(s) 450
SatI GCNGC 1 cut(s) 198
Sau3AI GATC 1 cut(s) 247
SchI GAGTC 1 cut(s) 101
ScrFI CCNGG 2 cut(s) 254, 380
SfaNI GCATC 3 cut(s) 10, 73, 484
SfcI CTRYAG 1 cut(s) 198
SmlI CTYRAG 1 cut(s) 449
SmoI CTYRAG 1 cut(s) 449
SnaBI TACGTA 1 cut(s) 598
Sse9I AATT 5 cut(s) 27, 349, 516, 525, 604
SsiI CCGC 1 cut(s) 86
SspMI CTAG 2 cut(s) 245, 327
StyD4I CCNGG 2 cut(s) 252, 378
TaaI ACNGT 2 cut(s) 318, 468
TaiI ACGT 2 cut(s) 102, 600
TaqII GACCGA 1 cut(s) 431
TasI AATT 5 cut(s) 27, 349, 516, 525, 604
TatI WGTACW 1 cut(s) 226
Tru1I TTAA 1 cut(s) 450
Tru9I TTAA 1 cut(s) 450
TseFI GTSAC 1 cut(s) 371
TseI GCWGC 1 cut(s) 197
Tsp45I GTSAC 1 cut(s) 371
TspDTI ATGAA 4 cut(s) 93, 141, 343, 422
Vha464I CTTAAG 1 cut(s) 449
XagI CCTNNNNNAGG 1 cut(s) 44
XapI RAATTY 1 cut(s) 27
XmiI GTMKAC 1 cut(s) 445
XspI CTAG 2 cut(s) 245, 327
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.