Rorug07G0129700

component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0001077
Physical Location & Seq
Reverse (-)
10277642 .. 10280738
3097 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0129700.1

Sequence Viewer

Length: 684 bp
ATGGCCGAGATAGCGCCGGCGAACTTCGTAGACCAATCAGTCGTTCCGGGAGATGTGGTTCTTGACCTAACAAAAATGGCGAACCAGACCATCAAACTGGGCGGCGGTCTCCGGCAGGACTGCGATGCAATAGCTGCGATGAAGGCCGGAACATTGAGGTTCTCAAAGCCCAACAAGTACTGGGTCGAAACGTCCCAGAAACGGTATGTGCCTTGTGTGGAGGATTCTGTTCTTGGAATCGTGGTGGACTCTAAAGCAGATAATTTTCTGGTGGACATTAAAGGGCCTTCGCTGGCGTTTTTGCCCGTGCTTGCTTTCGAAGGAGGAACCAGGAGAAACATACCAAAGTTTGAGGTAGGTACATTGCTTTATGTTCGGGTCGTGAAGGCAAACCCTGGTATGAACCCTGAGTTGTCATGCACTGATGCCGGTGGAAAAGCAGCAGAGTATGGTCCCCTTAAAGATGGCTACATGTTTGAATGCTCAACTGGCCTTTCAAGAATGCTGCTGAGTTCACCAACATGTCCAGTTCTTGCAGCACTTGGGAGGAACATCTCCTTTGAAATAGCAGTTGGTTTAAATGGCCGTGTTTGGGTGAACGCCACTGAAGCAGATAAGGTGATCATAGTTGCTAATGCAATTATGAACTCGGAGTCCCTTAGTGGGGTACAGCAGATTGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
eIF3_subunit

Protein Analysis

227

Amino Acids

24.2

Weight (kDa)

5.61

Isoelectric Point (pI)

42.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rrp40_N PF18311 31 - 68 1.1e-07 Exosome complex exonuclease Rrp40 N-terminal domain
RRP40_S1 PF21262 69 - 151 3.6e-27 Exosome complex component RRP40, S1 domain
KH_6 PF15985 156 - 204 4.6e-16 KH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 38
AccI GTMKAC 1 cut(s) 30
AciI CCGC 2 cut(s) 102, 105
AcoI YGGCCR 2 cut(s) 3, 583
AcuI CTGAAG 1 cut(s) 627
AfaI GTAC 3 cut(s) 179, 361, 669
AfiI CCNNNNNNNGG 4 cut(s) 201, 592, 663, 664
AflIII ACRYGT 2 cut(s) 471, 521
AgsI TTSAA 3 cut(s) 479, 498, 563
AjnI CCWGG 2 cut(s) 329, 394
AjuI GAANNNNNNNTTGG 4 cut(s) 27, 59, 555, 587
AloI GAACNNNNNNTCC 4 cut(s) 42, 74, 638, 670
AluBI AGCT 1 cut(s) 134
AluI AGCT 1 cut(s) 134
Alw26I GTCTC 1 cut(s) 113
AoxI GGCC 5 cut(s) 3, 144, 284, 490, 583
ApeKI GCWGC 4 cut(s) 134, 440, 505, 536
AspLEI GCGC 1 cut(s) 16
AspS9I GGNCC 2 cut(s) 284, 452
AsuC2I CCSGG 1 cut(s) 48
AsuHPI GGTGA 3 cut(s) 507, 607, 631
AsuII TTCGAA 1 cut(s) 318
AvaII GGWCC 1 cut(s) 452
BbvI GCAGC 4 cut(s) 121, 452, 492, 548
BccI CCATC 2 cut(s) 98, 458
BceAI ACGGC 1 cut(s) 570
BciT130I CCWGG 2 cut(s) 331, 396
BclI TGATCA 1 cut(s) 621
BcnI CCSGG 1 cut(s) 48
BcoDI GTCTC 1 cut(s) 113
BfoI RGCGCY 1 cut(s) 17
BisI GCNGC 5 cut(s) 103, 135, 441, 506, 537
BlsI GCNGC 5 cut(s) 104, 136, 442, 507, 538
BmcAI AGTACT 1 cut(s) 179
Bme1390I CCNGG 3 cut(s) 48, 331, 396
Bme18I GGWCC 1 cut(s) 452
BmgT120I GGNCC 2 cut(s) 284, 452
BmiI GGNNCC 2 cut(s) 328, 454
BmrFI CCNGG 3 cut(s) 48, 331, 396
BmrI ACTGGG 2 cut(s) 107, 190
BmsI GCATC 2 cut(s) 115, 415
BmuI ACTGGG 2 cut(s) 107, 190
Bpu14I TTCGAA 1 cut(s) 318
BpuMI CCSGG 1 cut(s) 48
BsaI GGTCTC 1 cut(s) 113
BsaJI CCNNGG 1 cut(s) 394
BsaXI ACNNNNNCTCC 2 cut(s) 42, 72
Bsc4I CCNNNNNNNGG 4 cut(s) 201, 592, 663, 664
Bse118I RCCGGY 2 cut(s) 16, 428
Bse1I ACTGG 4 cut(s) 102, 185, 493, 527
Bse3DI GCAATG 1 cut(s) 362
BseBI CCWGG 2 cut(s) 331, 396
BseDI CCNNGG 1 cut(s) 394
BseLI CCNNNNNNNGG 4 cut(s) 201, 592, 663, 664
BseMI GCAATG 1 cut(s) 362
BseMII CTCAG 2 cut(s) 399, 500
BseNI ACTGG 4 cut(s) 102, 185, 493, 527
BseXI GCAGC 4 cut(s) 121, 452, 492, 548
BshFI GGCC 5 cut(s) 5, 146, 286, 492, 585
BsiSI CCGG 5 cut(s) 17, 47, 112, 147, 429
BslFI GGGAC 3 cut(s) 178, 438, 640
BslI CCNNNNNNNGG 4 cut(s) 201, 592, 663, 664
BsmAI GTCTC 1 cut(s) 113
BsmFI GGGAC 3 cut(s) 178, 438, 640
BsmI GAATGC 2 cut(s) 485, 507
BsnI GGCC 5 cut(s) 5, 146, 286, 492, 585
Bso31I GGTCTC 1 cut(s) 113
Bsp119I TTCGAA 1 cut(s) 318
Bsp143I GATC 1 cut(s) 621
BspACI CCGC 2 cut(s) 102, 105
BspANI GGCC 5 cut(s) 5, 146, 286, 492, 585
BspCNI CTCAG 2 cut(s) 400, 501
BspLI GGNNCC 2 cut(s) 328, 454
BspT104I TTCGAA 1 cut(s) 318
BspTNI GGTCTC 1 cut(s) 113
BsrDI GCAATG 1 cut(s) 362
BsrFI RCCGGY 2 cut(s) 16, 428
BsrI ACTGG 4 cut(s) 102, 185, 493, 527
BssAI RCCGGY 2 cut(s) 16, 428
BssECI CCNNGG 1 cut(s) 394
BssMI GATC 1 cut(s) 621
Bst2UI CCWGG 2 cut(s) 331, 396
Bst4CI ACNGT 1 cut(s) 204
BstAPI GCANNNNNTGC 1 cut(s) 134
BstBI TTCGAA 1 cut(s) 318
BstC8I GCNNGC 3 cut(s) 18, 294, 312
BstDEI CTNAG 3 cut(s) 408, 509, 659
BstH2I RGCGCY 1 cut(s) 17
BstHHI GCGC 1 cut(s) 16
BstKTI GATC 1 cut(s) 624
BstMAI GTCTC 1 cut(s) 113
BstMBI GATC 1 cut(s) 621
BstMWI GCNNNNNNNGC 5 cut(s) 11, 134, 143, 489, 608
BstNI CCWGG 2 cut(s) 331, 396
BstNSI RCATGY 2 cut(s) 475, 525
BstSCI CCNGG 3 cut(s) 46, 329, 394
BstV1I GCAGC 4 cut(s) 121, 452, 492, 548
BstXI CCANNNNNNTGG 1 cut(s) 97
BsuRI GGCC 5 cut(s) 5, 146, 286, 492, 585
BtgZI GCGATG 2 cut(s) 138, 152
BtsIMutI CAGTG 2 cut(s) 420, 603
Cac8I GCNNGC 3 cut(s) 18, 294, 312
CfoI GCGC 1 cut(s) 16
Cfr10I RCCGGY 2 cut(s) 16, 428
Cfr13I GGNCC 2 cut(s) 284, 452
Csp6I GTAC 3 cut(s) 178, 360, 668
CviAII CATG 4 cut(s) 417, 472, 522, 681
CviJI RGCY 8 cut(s) 5, 134, 146, 169, 286, 468, 492, 585
CviKI_1 RGCY 8 cut(s) 5, 134, 146, 169, 286, 468, 492, 585
CviQI GTAC 3 cut(s) 178, 360, 668
DdeI CTNAG 3 cut(s) 408, 509, 659
DpnI GATC 1 cut(s) 623
DpnII GATC 1 cut(s) 621
DraI TTTAAA 1 cut(s) 579
DrdI GACNNNNNNGTC 1 cut(s) 38
DseDI GACNNNNNNGTC 1 cut(s) 38
EaeI YGGCCR 2 cut(s) 3, 583
Eco31I GGTCTC 1 cut(s) 113
Eco47I GGWCC 1 cut(s) 452
Eco57I CTGAAG 1 cut(s) 627
EcoO109I RGGNCCY 1 cut(s) 284
EcoRII CCWGG 2 cut(s) 329, 394
FaeI CATG 4 cut(s) 420, 475, 525, 684
FaqI GGGAC 3 cut(s) 178, 438, 640
FatI CATG 4 cut(s) 416, 471, 521, 680
FbaI TGATCA 1 cut(s) 621
FblI GTMKAC 1 cut(s) 30
Fnu4HI GCNGC 5 cut(s) 103, 135, 441, 506, 537
Fsp4HI GCNGC 5 cut(s) 103, 135, 441, 506, 537
GlaI GCGC 1 cut(s) 15
GluI GCNGC 5 cut(s) 103, 135, 441, 506, 537
HaeII RGCGCY 1 cut(s) 17
HaeIII GGCC 5 cut(s) 5, 146, 286, 492, 585
HapII CCGG 5 cut(s) 17, 47, 112, 147, 429
HhaI GCGC 1 cut(s) 16
Hin1II CATG 4 cut(s) 420, 475, 525, 684
Hin6I GCGC 1 cut(s) 14
HinP1I GCGC 1 cut(s) 14
HinfI GANTC 4 cut(s) 224, 237, 248, 653
HpaII CCGG 5 cut(s) 17, 47, 112, 147, 429
HphI GGTGA 3 cut(s) 507, 607, 631
Hpy166II GTNNAC 5 cut(s) 31, 247, 274, 515, 598
Hpy188I TCNGA 1 cut(s) 652
Hpy188III TCNNGA 3 cut(s) 62, 382, 498
Hpy8I GTNNAC 5 cut(s) 31, 247, 274, 515, 598
HpyAV CCTTC 4 cut(s) 136, 297, 314, 379
HpyCH4III ACNGT 1 cut(s) 204
HpyCH4IV ACGT 1 cut(s) 191
HpyCH4V TGCA 5 cut(s) 128, 420, 536, 638, 680
HpyF10VI GCNNNNNNNGC 5 cut(s) 11, 134, 143, 489, 608
HpyF3I CTNAG 3 cut(s) 408, 509, 659
HpySE526I ACGT 1 cut(s) 191
Hsp92II CATG 4 cut(s) 420, 475, 525, 684
HspAI GCGC 1 cut(s) 14
KroI GCCGGC 1 cut(s) 16
KroNI GCCGGC 1 cut(s) 18
Ksp22I TGATCA 1 cut(s) 621
Kzo9I GATC 1 cut(s) 621
Lsp1109I GCAGC 4 cut(s) 121, 452, 492, 548
LweI GCATC 2 cut(s) 115, 415
MaeII ACGT 1 cut(s) 191
MalI GATC 1 cut(s) 623
MboI GATC 1 cut(s) 621
MluCI AATT 2 cut(s) 262, 639
MlyI GAGTC 2 cut(s) 242, 662
MnlI CCTC 5 cut(s) 150, 214, 317, 346, 540
MreI CGCCGGCG 1 cut(s) 16
MroNI GCCGGC 1 cut(s) 16
MseI TTAA 3 cut(s) 279, 459, 578
MslI CAYNNNNRTG 1 cut(s) 520
MspI CCGG 5 cut(s) 17, 47, 112, 147, 429
MspR9I CCNGG 3 cut(s) 48, 331, 396
Mva1269I GAATGC 2 cut(s) 485, 507
MvaI CCWGG 2 cut(s) 331, 396
MwoI GCNNNNNNNGC 5 cut(s) 11, 134, 143, 489, 608
NaeI GCCGGC 1 cut(s) 18
NciI CCSGG 1 cut(s) 48
NdeII GATC 1 cut(s) 621
NgoMIV GCCGGC 1 cut(s) 16
NlaIII CATG 4 cut(s) 420, 475, 525, 684
NlaIV GGNNCC 2 cut(s) 328, 454
NmeAIII GCCGAG 1 cut(s) 31
NspI RCATGY 2 cut(s) 475, 525
NspV TTCGAA 1 cut(s) 318
PciI ACATGT 2 cut(s) 471, 521
PctI GAATGC 2 cut(s) 485, 507
PdiI GCCGGC 1 cut(s) 18
PfeI GAWTC 2 cut(s) 224, 237
PfoI TCCNGGA 1 cut(s) 46
PkrI GCNGC 5 cut(s) 104, 136, 442, 507, 538
PleI GAGTC 2 cut(s) 242, 661
PpsI GAGTC 2 cut(s) 242, 661
PscI ACATGT 2 cut(s) 471, 521
Psp6I CCWGG 2 cut(s) 329, 394
PspGI CCWGG 2 cut(s) 329, 394
PspN4I GGNNCC 2 cut(s) 328, 454
PspPI GGNCC 2 cut(s) 284, 452
RsaI GTAC 3 cut(s) 179, 361, 669
RsaNI GTAC 3 cut(s) 178, 360, 668
RseI CAYNNNNRTG 1 cut(s) 520
SaqAI TTAA 3 cut(s) 279, 459, 578
SatI GCNGC 5 cut(s) 103, 135, 441, 506, 537
Sau3AI GATC 1 cut(s) 621
Sau96I GGNCC 2 cut(s) 284, 452
ScaI AGTACT 1 cut(s) 179
SchI GAGTC 2 cut(s) 242, 662
ScrFI CCNGG 3 cut(s) 48, 331, 396
SetI ASST 7 cut(s) 69, 136, 161, 194, 357, 361, 621
SfaNI GCATC 2 cut(s) 115, 415
SfuI TTCGAA 1 cut(s) 318
SgrAI CRCCGGYG 1 cut(s) 16
SinI GGWCC 1 cut(s) 452
SmiMI CAYNNNNRTG 1 cut(s) 520
Sse9I AATT 2 cut(s) 262, 639
SsiI CCGC 2 cut(s) 102, 105
StyD4I CCNGG 3 cut(s) 46, 329, 394
TaaI ACNGT 1 cut(s) 204
TaiI ACGT 1 cut(s) 194
TaqI TCGA 2 cut(s) 186, 318
TasI AATT 2 cut(s) 262, 639
TatI WGTACW 1 cut(s) 177
TauI GCSGC 1 cut(s) 105
TfiI GAWTC 2 cut(s) 224, 237
Tru1I TTAA 3 cut(s) 279, 459, 578
Tru9I TTAA 3 cut(s) 279, 459, 578
TscAI CASTG 2 cut(s) 427, 610
TseI GCWGC 4 cut(s) 134, 440, 505, 536
TspDTI ATGAA 3 cut(s) 155, 416, 659
TspRI CASTG 2 cut(s) 427, 610
VpaK11BI GGWCC 1 cut(s) 452
XceI RCATGY 2 cut(s) 475, 525
XmiI GTMKAC 1 cut(s) 30
ZrmI AGTACT 1 cut(s) 179
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.