MD06G1077500.v1.1

Salutaridine reductase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0001077
Physical Location & Seq
Forward (+)
19222869 .. 19224715
1847 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1077500.v1.1.491

Sequence Viewer

Length: 912 bp
ATGGCAGAAATCAGTTTCGGGTCTAAGAGGATTGCGGTTGTTACTGGAGGCAACAAAGGGATTGGATTTGAGATTAGCAGGCAATTAGCAGCTAATGGAGTTGGGGTGGTGTTAACTGCAAGAGATGTGAGGAGGGGAACAGAAGCCGTTGAGAAATTGAAGGCTTCTTATGGCCTCTCTGATGTGGTATTTCATCAGCTTGATGTAACTGACCCATCTAGCATTGCCTCTCTGGCTGAATTTCTCAAAACTCAACTTGGAAAACTTGACATTTTGGTAAATAATGCAGCAGTTGTCGGGTCCATATTCCTCACAGACGACAAAGAGAAGTTGGCAATTGATGATCTTATAGGTCCCAAAGCAGCAAATAAGAATCAAGTCCTAAAACAAACCTACGAGACAGCAGGGGATTGTTTAAAAACAAACTACTATGGAATTAAGCAACTCACGGAAGCACTCATTCCACTTCTTCAACAATCTGACTCGGCAAGGATCGTCAATGTCTCCTCCCAATTGGGACAACTAAGGGTTATTCCAAACGAGAAAGCGAAGAAGGAGCTAGTAGATGGTGATAGCCTCACAGAAGAGAAAGTGGACAACCTTGTTGAGGGATTTTTGGAGGATGTGAAGCAGAATTTGGTTGAAGCCAAAAGCTGGCCAACCAATTTCTCTGCCTACATTGTATCCAAAGCCGCTCTGAATGCTTACACAAGAGTTTTGGCTAAGAAGTATTCCAGCAAAATTGCTATCAATGCCGTGTGTCCGGGTTTTACCGACACGGACTTGAACAATCACACTGGAGTCTTCACAACTGAAGAAGCTGCAAAAGGTCCTGTGAAGCTGGCTTTGCTGCCTGATAATGGAGCTTCTGGCCTCTTCTTTGCACAGACTGAACTATCAACTTTCGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00902 ko01110 map00902 map01110
Pfam Domains
Protein Families
adh_short adh_short_C2

Protein Analysis

304

Amino Acids

32.6

Weight (kDa)

5.47

Isoelectric Point (pI)

23.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
adh_short PF00106 11 - 177 3.9e-27 short chain dehydrogenase
KR PF08659 13 - 130 2.2e-08 KR domain
adh_short_C2 PF13561 18 - 182 2.5e-19 Enoyl-(Acyl carrier protein) reductase
adh_short_C2 PF13561 220 - 268 1.1e-08 Enoyl-(Acyl carrier protein) reductase
adh_short PF00106 221 - 265 3.2e-08 short chain dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 654
AccBSI CCGCTC 1 cut(s) 695
AciI CCGC 2 cut(s) 35, 693
AclWI GGATC 1 cut(s) 500
AcoI YGGCCR 1 cut(s) 656
AcsI RAATTY 2 cut(s) 239, 634
AcuI CTGAAG 1 cut(s) 834
AfiI CCNNNNNNNGG 3 cut(s) 607, 654, 860
AgsI TTSAA 4 cut(s) 160, 473, 644, 787
AjuI GAANNNNNNNTTGG 2 cut(s) 620, 652
AluBI AGCT 7 cut(s) 92, 199, 559, 654, 821, 841, 866
AluI AGCT 7 cut(s) 92, 199, 559, 654, 821, 841, 866
Alw26I GTCTC 2 cut(s) 392, 508
AlwI GGATC 1 cut(s) 500
AoxI GGCC 3 cut(s) 172, 656, 871
ApeKI GCWGC 5 cut(s) 89, 287, 362, 821, 850
ApoI RAATTY 2 cut(s) 239, 634
AspS9I GGNCC 3 cut(s) 300, 353, 830
AsuC2I CCSGG 1 cut(s) 765
AsuHPI GGTGA 1 cut(s) 581
AsuII TTCGAA 1 cut(s) 906
AvaII GGWCC 3 cut(s) 300, 353, 830
BalI TGGCCA 1 cut(s) 658
BbsI GAAGAC 1 cut(s) 796
BbvI GCAGC 5 cut(s) 101, 299, 374, 808, 837
BccI CCATC 2 cut(s) 223, 560
BceAI ACGGC 2 cut(s) 131, 740
BciVI GTATCC 1 cut(s) 694
BcnI CCSGG 1 cut(s) 765
BcoDI GTCTC 2 cut(s) 392, 508
BfaI CTAG 2 cut(s) 219, 560
BfuI GTATCC 1 cut(s) 694
BglI GCCNNNNNGGC 1 cut(s) 233
BisI GCNGC 6 cut(s) 90, 288, 363, 693, 822, 851
BlsI GCNGC 6 cut(s) 91, 289, 364, 694, 823, 852
Bme1390I CCNGG 1 cut(s) 765
Bme18I GGWCC 3 cut(s) 300, 353, 830
BmgT120I GGNCC 3 cut(s) 300, 353, 830
BmiI GGNNCC 2 cut(s) 301, 355
BmrFI CCNGG 1 cut(s) 765
BpiI GAAGAC 1 cut(s) 796
BpmI CTGGAG 2 cut(s) 66, 819
Bpu14I TTCGAA 1 cut(s) 906
BpuMI CCSGG 1 cut(s) 765
BsaXI ACNNNNNCTCC 2 cut(s) 90, 120
Bsc4I CCNNNNNNNGG 3 cut(s) 607, 654, 860
Bse1I ACTGG 2 cut(s) 49, 802
Bse3DI GCAATG 1 cut(s) 222
BseGI GGATG 1 cut(s) 628
BseLI CCNNNNNNNGG 3 cut(s) 607, 654, 860
BseMI GCAATG 1 cut(s) 222
BseNI ACTGG 2 cut(s) 49, 802
BseRI GAGGAG 2 cut(s) 145, 496
BseXI GCAGC 5 cut(s) 101, 299, 374, 808, 837
BshFI GGCC 3 cut(s) 174, 658, 873
BsiSI CCGG 1 cut(s) 764
BslFI GGGAC 2 cut(s) 339, 531
BslI CCNNNNNNNGG 3 cut(s) 607, 654, 860
BsmAI GTCTC 2 cut(s) 392, 508
BsmFI GGGAC 2 cut(s) 339, 531
BsmI GAATGC 1 cut(s) 706
BsnI GGCC 3 cut(s) 174, 658, 873
Bsp119I TTCGAA 1 cut(s) 906
Bsp143I GATC 2 cut(s) 343, 492
BspACI CCGC 2 cut(s) 35, 693
BspANI GGCC 3 cut(s) 174, 658, 873
BspLI GGNNCC 2 cut(s) 301, 355
BspPI GGATC 1 cut(s) 500
BspT104I TTCGAA 1 cut(s) 906
BsrBI CCGCTC 1 cut(s) 695
BsrDI GCAATG 1 cut(s) 222
BsrI ACTGG 2 cut(s) 49, 802
BssMI GATC 2 cut(s) 343, 492
Bst6I CTCTTC 2 cut(s) 579, 881
BstBI TTCGAA 1 cut(s) 906
BstC8I GCNNGC 3 cut(s) 80, 656, 843
BstDEI CTNAG 3 cut(s) 24, 524, 723
BstENI CCTNNNNNAGG 1 cut(s) 605
BstF5I GGATG 1 cut(s) 628
BstKTI GATC 2 cut(s) 346, 495
BstMAI GTCTC 2 cut(s) 392, 508
BstMBI GATC 2 cut(s) 343, 492
BstMWI GCNNNNNNNGC 4 cut(s) 233, 701, 752, 847
BstSCI CCNGG 1 cut(s) 763
BstV1I GCAGC 5 cut(s) 101, 299, 374, 808, 837
BstV2I GAAGAC 1 cut(s) 796
BsuI GTATCC 1 cut(s) 694
BsuRI GGCC 3 cut(s) 174, 658, 873
BtsCI GGATG 1 cut(s) 628
BtsIMutI CAGTG 1 cut(s) 795
Cac8I GCNNGC 3 cut(s) 80, 656, 843
Cfr13I GGNCC 3 cut(s) 300, 353, 830
DdeI CTNAG 3 cut(s) 24, 524, 723
DpnI GATC 2 cut(s) 345, 494
DpnII GATC 2 cut(s) 343, 492
DraI TTTAAA 1 cut(s) 417
EaeI YGGCCR 1 cut(s) 656
Eam1104I CTCTTC 2 cut(s) 579, 881
EarI CTCTTC 2 cut(s) 579, 881
Eco47I GGWCC 3 cut(s) 300, 353, 830
Eco57I CTGAAG 1 cut(s) 834
EcoNI CCTNNNNNAGG 1 cut(s) 605
EcoO109I RGGNCCY 2 cut(s) 353, 830
FaiI YATR 4 cut(s) 171, 305, 350, 432
FaqI GGGAC 2 cut(s) 339, 531
Fnu4HI GCNGC 6 cut(s) 90, 288, 363, 693, 822, 851
FokI GGATG 1 cut(s) 635
Fsp4HI GCNGC 6 cut(s) 90, 288, 363, 693, 822, 851
FspBI CTAG 2 cut(s) 219, 560
GluI GCNGC 6 cut(s) 90, 288, 363, 693, 822, 851
GsuI CTGGAG 2 cut(s) 66, 819
HaeIII GGCC 3 cut(s) 174, 658, 873
HapII CCGG 1 cut(s) 764
HincII GTYRAC 1 cut(s) 114
HindII GTYRAC 1 cut(s) 114
HinfI GANTC 3 cut(s) 373, 482, 801
HpaI GTTAAC 1 cut(s) 114
HpaII CCGG 1 cut(s) 764
HphI GGTGA 1 cut(s) 581
Hpy166II GTNNAC 2 cut(s) 114, 595
Hpy188I TCNGA 3 cut(s) 181, 481, 699
Hpy8I GTNNAC 2 cut(s) 114, 595
HpyAV CCTTC 2 cut(s) 154, 547
HpyCH4V TGCA 4 cut(s) 119, 287, 824, 884
HpyF10VI GCNNNNNNNGC 4 cut(s) 233, 701, 752, 847
HpyF3I CTNAG 3 cut(s) 24, 524, 723
KspAI GTTAAC 1 cut(s) 114
Kzo9I GATC 2 cut(s) 343, 492
LmnI GCTCC 2 cut(s) 556, 863
Lsp1109I GCAGC 5 cut(s) 101, 299, 374, 808, 837
MaeI CTAG 2 cut(s) 219, 560
MaeIII GTNAC 2 cut(s) 40, 205
MalI GATC 2 cut(s) 345, 494
MbiI CCGCTC 1 cut(s) 695
MboI GATC 2 cut(s) 343, 492
MboII GAAGA 6 cut(s) 461, 562, 596, 796, 827, 868
MfeI CAATTG 2 cut(s) 336, 512
MlsI TGGCCA 1 cut(s) 658
MluCI AATT 9 cut(s) 83, 155, 239, 336, 435, 512, 634, 664, 741
MluNI TGGCCA 1 cut(s) 658
MlyI GAGTC 2 cut(s) 476, 810
Mox20I TGGCCA 1 cut(s) 658
MscI TGGCCA 1 cut(s) 658
MseI TTAA 3 cut(s) 113, 416, 438
Msp20I TGGCCA 1 cut(s) 658
MspI CCGG 1 cut(s) 764
MspR9I CCNGG 1 cut(s) 765
MunI CAATTG 2 cut(s) 336, 512
Mva1269I GAATGC 1 cut(s) 706
MwoI GCNNNNNNNGC 4 cut(s) 233, 701, 752, 847
NciI CCSGG 1 cut(s) 765
NdeII GATC 2 cut(s) 343, 492
NlaIV GGNNCC 2 cut(s) 301, 355
NmeAIII GCCGAG 1 cut(s) 464
NspV TTCGAA 1 cut(s) 906
PctI GAATGC 1 cut(s) 706
PfeI GAWTC 1 cut(s) 373
PflMI CCANNNNNTGG 1 cut(s) 654
PkrI GCNGC 6 cut(s) 91, 289, 364, 694, 823, 852
PleI GAGTC 2 cut(s) 476, 809
PpsI GAGTC 2 cut(s) 476, 809
PpuMI RGGWCCY 2 cut(s) 353, 830
Psp5II RGGWCCY 2 cut(s) 353, 830
PspN4I GGNNCC 2 cut(s) 301, 355
PspPI GGNCC 3 cut(s) 300, 353, 830
PspPPI RGGWCCY 2 cut(s) 353, 830
SaqAI TTAA 3 cut(s) 113, 416, 438
SatI GCNGC 6 cut(s) 90, 288, 363, 693, 822, 851
Sau3AI GATC 2 cut(s) 343, 492
Sau96I GGNCC 3 cut(s) 300, 353, 830
SchI GAGTC 2 cut(s) 476, 810
ScrFI CCNGG 1 cut(s) 765
SfuI TTCGAA 1 cut(s) 906
SinI GGWCC 3 cut(s) 300, 353, 830
Sse9I AATT 9 cut(s) 83, 155, 239, 336, 435, 512, 634, 664, 741
SsiI CCGC 2 cut(s) 35, 693
SspMI CTAG 2 cut(s) 219, 560
StyD4I CCNGG 1 cut(s) 763
TaqI TCGA 1 cut(s) 906
TasI AATT 9 cut(s) 83, 155, 239, 336, 435, 512, 634, 664, 741
TauI GCSGC 1 cut(s) 695
TfiI GAWTC 1 cut(s) 373
Tru1I TTAA 3 cut(s) 113, 416, 438
Tru9I TTAA 3 cut(s) 113, 416, 438
TscAI CASTG 1 cut(s) 802
TseI GCWGC 5 cut(s) 89, 287, 362, 821, 850
TspDTI ATGAA 1 cut(s) 182
TspGWI ACGGA 2 cut(s) 464, 794
TspRI CASTG 1 cut(s) 802
Van91I CCANNNNNTGG 1 cut(s) 654
VpaK11BI GGWCC 3 cut(s) 300, 353, 830
XagI CCTNNNNNAGG 1 cut(s) 605
XapI RAATTY 2 cut(s) 239, 634
XspI CTAG 2 cut(s) 219, 560
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.