pycom06g07550

Salutaridine reductase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0001077
Physical Location & Seq
Forward (+)
12314268 .. 12315487
1220 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g07550.3

Sequence Viewer

Length: 675 bp
ATGTTTGAGTCTGCCGACGAAAGAAAGGGATGCAGCACGAGAACTTCCAAGCTTCAACGTTCCAATGCCGGATCCGACAATGAAGTTGTGAAGCAAACCTATGAGACAGCAAGGGATTGCCTGAAAACAAACTATTATGGAGTTAAGCAACTTACCGAAGCACTTATTCCACTGCTTCAAAAATCCAACTCGGCAACGATAGTCAATGTCTCCTCCTCGGTGGGAAAGCTAAGAGTACAGTACCGAAACCCGAATCTGAAATCCATCAATTGTTTCAACTGCATGTCTGAGTTTGGAATACCAAATGAGAAAGCAAAGAGGGTTCTAGGAGATACTAACAGCCTCAGAGAAGAGGAAGTGGACAACCTAGTTGAGGAATTTATGGAGGATGTGAAGCAGAGTTTGGTTGAAGCCAAAGTCTGGCCTGCCAAGTTATCTGCCTACATCGTATCCAAAGCAGCTCTGAATGCTTATACAAGAGTCTTGGCAAAGATGTATCCCGACATTGCTATTAACGCAGTCAGTCCTAGTTTTACCAGAACGGAATTAAACAAGAATACTGGGGTGCTTACAGTTGAAGAAGGCGCAAAAGGTCCTGTAAAGCTAGCTTTGTTGCCTGACAATCGAGTTTCTGGCCTCTACTTCGAACAGACTGAATTGTCAACCTTTGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00902 ko01110 map00902 map01110
Pfam Domains
Protein Families
adh_short adh_short_C2

Protein Analysis

225

Amino Acids

24.91

Weight (kDa)

7.56

Isoelectric Point (pI)

39.17

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 658
AccB7I CCANNNNNTGG 1 cut(s) 420
AclI AACGTT 1 cut(s) 58
AclWI GGATC 2 cut(s) 66, 79
AcsI RAATTY 1 cut(s) 377
AfaI GTAC 2 cut(s) 237, 242
AfiI CCNNNNNNNGG 2 cut(s) 373, 420
AgsI TTSAA 5 cut(s) 56, 179, 277, 410, 578
AjuI GAANNNNNNNTTGG 2 cut(s) 386, 418
AluBI AGCT 5 cut(s) 52, 229, 461, 604, 608
AluI AGCT 5 cut(s) 52, 229, 461, 604, 608
Alw26I GTCTC 2 cut(s) 98, 214
AlwI GGATC 2 cut(s) 66, 79
AoxI GGCC 2 cut(s) 422, 634
ApeKI GCWGC 2 cut(s) 33, 458
ApoI RAATTY 1 cut(s) 377
AspLEI GCGC 1 cut(s) 587
AspS9I GGNCC 1 cut(s) 593
AsuII TTCGAA 1 cut(s) 645
AsuNHI GCTAGC 1 cut(s) 604
AvaII GGWCC 1 cut(s) 593
BamHI GGATCC 1 cut(s) 71
BauI CACGAG 1 cut(s) 37
BbvI GCAGC 2 cut(s) 45, 470
BccI CCATC 1 cut(s) 272
BciVI GTATCC 2 cut(s) 460, 507
BcoDI GTCTC 2 cut(s) 98, 214
BfaI CTAG 4 cut(s) 326, 368, 528, 605
BfuI GTATCC 2 cut(s) 460, 507
BisI GCNGC 2 cut(s) 34, 459
BlsI GCNGC 2 cut(s) 35, 460
Bme18I GGWCC 1 cut(s) 593
BmgT120I GGNCC 1 cut(s) 593
BmiI GGNNCC 1 cut(s) 73
BmrI ACTGGG 1 cut(s) 570
BmsI GCATC 1 cut(s) 20
BmtI GCTAGC 1 cut(s) 608
BmuI ACTGGG 1 cut(s) 570
Bpu14I TTCGAA 1 cut(s) 645
BsaJI CCNNGG 1 cut(s) 216
Bsc4I CCNNNNNNNGG 2 cut(s) 373, 420
Bse1I ACTGG 1 cut(s) 565
Bse3DI GCAATG 1 cut(s) 504
BseDI CCNNGG 1 cut(s) 216
BseGI GGATG 2 cut(s) 35, 394
BseLI CCNNNNNNNGG 2 cut(s) 373, 420
BseMI GCAATG 1 cut(s) 504
BseMII CTCAG 2 cut(s) 279, 358
BseNI ACTGG 1 cut(s) 565
BseRI GAGGAG 2 cut(s) 202, 205
BseXI GCAGC 2 cut(s) 45, 470
BshFI GGCC 2 cut(s) 424, 636
BsiSI CCGG 1 cut(s) 69
BslI CCNNNNNNNGG 2 cut(s) 373, 420
BsmAI GTCTC 2 cut(s) 98, 214
BsmI GAATGC 1 cut(s) 472
BsnI GGCC 2 cut(s) 424, 636
Bsp119I TTCGAA 1 cut(s) 645
Bsp143I GATC 1 cut(s) 71
BspANI GGCC 2 cut(s) 424, 636
BspCNI CTCAG 2 cut(s) 280, 357
BspLI GGNNCC 1 cut(s) 73
BspOI GCTAGC 1 cut(s) 608
BspPI GGATC 2 cut(s) 66, 79
BspT104I TTCGAA 1 cut(s) 645
BsrDI GCAATG 1 cut(s) 504
BsrI ACTGG 1 cut(s) 565
BssECI CCNNGG 1 cut(s) 216
BssMI GATC 1 cut(s) 71
BssSI CACGAG 1 cut(s) 37
Bst2BI CACGAG 1 cut(s) 37
Bst4CI ACNGT 2 cut(s) 240, 574
Bst6I CTCTTC 1 cut(s) 345
BstBI TTCGAA 1 cut(s) 645
BstC8I GCNNGC 2 cut(s) 426, 606
BstDEI CTNAG 3 cut(s) 230, 288, 344
BstENI CCTNNNNNAGG 1 cut(s) 371
BstF5I GGATG 2 cut(s) 35, 394
BstHHI GCGC 1 cut(s) 587
BstKTI GATC 1 cut(s) 74
BstMAI GTCTC 2 cut(s) 98, 214
BstMBI GATC 1 cut(s) 71
BstMWI GCNNNNNNNGC 2 cut(s) 467, 515
BstNSI RCATGY 1 cut(s) 286
BstV1I GCAGC 2 cut(s) 45, 470
BstX2I RGATCY 1 cut(s) 71
BstYI RGATCY 1 cut(s) 71
BsuI GTATCC 2 cut(s) 460, 507
BsuRI GGCC 2 cut(s) 424, 636
BtsCI GGATG 2 cut(s) 35, 394
BtsI GCAGTG 1 cut(s) 170
BtsIMutI CAGTG 1 cut(s) 170
Cac8I GCNNGC 2 cut(s) 426, 606
CfoI GCGC 1 cut(s) 587
Cfr13I GGNCC 1 cut(s) 593
Csp6I GTAC 2 cut(s) 236, 241
CviAII CATG 1 cut(s) 283
CviJI RGCY 9 cut(s) 52, 229, 342, 413, 424, 461, 604, 608, 636
CviKI_1 RGCY 9 cut(s) 52, 229, 342, 413, 424, 461, 604, 608, 636
CviQI GTAC 2 cut(s) 236, 241
DdeI CTNAG 3 cut(s) 230, 288, 344
DpnI GATC 1 cut(s) 73
DpnII GATC 1 cut(s) 71
DrdI GACNNNNNNGTC 1 cut(s) 658
DseDI GACNNNNNNGTC 1 cut(s) 658
Eam1104I CTCTTC 1 cut(s) 345
EarI CTCTTC 1 cut(s) 345
Eco47I GGWCC 1 cut(s) 593
EcoNI CCTNNNNNAGG 1 cut(s) 371
EcoO109I RGGNCCY 1 cut(s) 593
FaeI CATG 1 cut(s) 286
FaiI YATR 5 cut(s) 102, 138, 284, 383, 474
FatI CATG 1 cut(s) 282
Fnu4HI GCNGC 2 cut(s) 34, 459
FokI GGATG 2 cut(s) 42, 401
Fsp4HI GCNGC 2 cut(s) 34, 459
FspBI CTAG 4 cut(s) 326, 368, 528, 605
GlaI GCGC 1 cut(s) 586
GluI GCNGC 2 cut(s) 34, 459
HaeIII GGCC 2 cut(s) 424, 636
HapII CCGG 1 cut(s) 69
HhaI GCGC 1 cut(s) 587
Hin1II CATG 1 cut(s) 286
Hin6I GCGC 1 cut(s) 585
HinP1I GCGC 1 cut(s) 585
HincII GTYRAC 1 cut(s) 663
HindII GTYRAC 1 cut(s) 663
HindIII AAGCTT 1 cut(s) 50
HinfI GANTC 3 cut(s) 8, 253, 480
HpaII CCGG 1 cut(s) 69
Hpy166II GTNNAC 2 cut(s) 361, 663
Hpy188I TCNGA 5 cut(s) 76, 258, 289, 347, 465
Hpy188III TCNNGA 1 cut(s) 500
Hpy8I GTNNAC 2 cut(s) 361, 663
Hpy99I CGWCG 1 cut(s) 20
HpyAV CCTTC 1 cut(s) 575
HpyCH4III ACNGT 2 cut(s) 240, 574
HpyCH4IV ACGT 1 cut(s) 58
HpyCH4V TGCA 2 cut(s) 33, 282
HpyF10VI GCNNNNNNNGC 2 cut(s) 467, 515
HpyF3I CTNAG 3 cut(s) 230, 288, 344
HpySE526I ACGT 1 cut(s) 58
Hsp92II CATG 1 cut(s) 286
HspAI GCGC 1 cut(s) 585
Kzo9I GATC 1 cut(s) 71
LpnPI CCDG 9 cut(s) 82, 134, 406, 438, 546, 550, 609, 618, 630
Lsp1109I GCAGC 2 cut(s) 45, 470
LweI GCATC 1 cut(s) 20
MaeI CTAG 4 cut(s) 326, 368, 528, 605
MaeII ACGT 1 cut(s) 58
MalI GATC 1 cut(s) 73
MboI GATC 1 cut(s) 71
MboII GAAGA 2 cut(s) 362, 590
MfeI CAATTG 1 cut(s) 268
MflI RGATCY 1 cut(s) 71
MluCI AATT 4 cut(s) 268, 377, 545, 656
MlyI GAGTC 2 cut(s) 17, 489
MmeI TCCRAC 2 cut(s) 99, 210
MnlI CCTC 8 cut(s) 223, 226, 312, 346, 353, 367, 379, 647
MseI TTAA 3 cut(s) 144, 513, 548
MspI CCGG 1 cut(s) 69
MunI CAATTG 1 cut(s) 268
Mva1269I GAATGC 1 cut(s) 472
MwoI GCNNNNNNNGC 2 cut(s) 467, 515
NdeII GATC 1 cut(s) 71
NheI GCTAGC 1 cut(s) 604
NlaIII CATG 1 cut(s) 286
NlaIV GGNNCC 1 cut(s) 73
NmeAIII GCCGAG 1 cut(s) 170
NspI RCATGY 1 cut(s) 286
NspV TTCGAA 1 cut(s) 645
PctI GAATGC 1 cut(s) 472
PfeI GAWTC 1 cut(s) 253
PflMI CCANNNNNTGG 1 cut(s) 420
PkrI GCNGC 2 cut(s) 35, 460
PleI GAGTC 2 cut(s) 16, 488
PpsI GAGTC 2 cut(s) 16, 488
PpuMI RGGWCCY 1 cut(s) 593
Psp1406I AACGTT 1 cut(s) 58
Psp5II RGGWCCY 1 cut(s) 593
PspN4I GGNNCC 1 cut(s) 73
PspPI GGNCC 1 cut(s) 593
PspPPI RGGWCCY 1 cut(s) 593
PsuI RGATCY 1 cut(s) 71
RsaI GTAC 2 cut(s) 237, 242
RsaNI GTAC 2 cut(s) 236, 241
SaqAI TTAA 3 cut(s) 144, 513, 548
SatI GCNGC 2 cut(s) 34, 459
Sau3AI GATC 1 cut(s) 71
Sau96I GGNCC 1 cut(s) 593
SchI GAGTC 2 cut(s) 17, 489
SfaNI GCATC 1 cut(s) 20
SfuI TTCGAA 1 cut(s) 645
SinI GGWCC 1 cut(s) 593
Sse9I AATT 4 cut(s) 268, 377, 545, 656
SspMI CTAG 4 cut(s) 326, 368, 528, 605
TaaI ACNGT 2 cut(s) 240, 574
TaiI ACGT 1 cut(s) 61
TaqI TCGA 2 cut(s) 625, 645
TasI AATT 4 cut(s) 268, 377, 545, 656
TatI WGTACW 1 cut(s) 235
TfiI GAWTC 1 cut(s) 253
Tru1I TTAA 3 cut(s) 144, 513, 548
Tru9I TTAA 3 cut(s) 144, 513, 548
TscAI CASTG 1 cut(s) 177
TseI GCWGC 2 cut(s) 33, 458
TspDTI ATGAA 1 cut(s) 96
TspGWI ACGGA 1 cut(s) 557
TspRI CASTG 1 cut(s) 177
Van91I CCANNNNNTGG 1 cut(s) 420
VpaK11BI GGWCC 1 cut(s) 593
XagI CCTNNNNNAGG 1 cut(s) 371
XapI RAATTY 1 cut(s) 377
XceI RCATGY 1 cut(s) 286
XspI CTAG 4 cut(s) 326, 368, 528, 605
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.