Rw7G015020

Salutaridine reductase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0001077
Physical Location & Seq
Reverse (-)
14904507 .. 14906918
2412 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G015020.1

Sequence Viewer

Length: 897 bp
ATGGTGGCAATCTGCTTGATCCGCAAGTCATGCACGAGTGGATCCACAACGATTGCAGTTGTTACTGGAGCCAACAAAGGGATTGGACTTGAGATTAGTAAGCAATTAGCTTCTAAAGGAGTTGGGGTGGTATTAACAGCAAGAGATGCGAAGAGAGGAACAGAAGCAGCTGAAAGCCTAAAGGCTTCTGGTTTCTCCGATGTGGTCTTCCATCAGCTAGATGTAACTGCCCCAACTAGCATTGCTTCTTTGGCAAACTTTCTCAAAACGCAATTCAGAAAGCTTGACATATTGGTTAACAATGCAGGAGTCCTTGGATCTGTATACCTCGCAGACGACATAGTCGGGCCTAACAATGTTAAACAAAATAAAGTGCAAACTTACGAGACAGCGGCGGATTGTTTGAAAACAAACTATTATGGAATCAAGCAACTCACAGAAGCACTTGTTCCACTACTTCAAAAATCTGAAGCTCCAAAGATAGTCAATGTCTCTTCCTCACGGGGACAGCTGAGACTTATTTCAAATGAGAAAGCAAAGAAGGAGCTAGGAGATGTGGATAACCTCACCGAGGAGAAAGTGGACAAGCTAGTCGAGGAATTTCTAGAGGATGTGAAGCATGATTCGATAAAATCCAAAGGATGGCCTTTAGATATATCTGCCTACATTGTATCAAAAGCAGCTCTTAATGCTTATACAAGAGTCGTGGCAAAGAAGTATCCCCACATTGCTATAAACGCAGTTGGTCCAGGTTATACCAAAACAGATCTCAACAATAATACCGGGATTCTCACAGTTGAAGAAGCTGCACTAGGTCCTGTGAAGCTGGCTTTGATAGCCGAAACTGGAATTTCAGGCCGCTTCTTTAACCGGAATGAAGAGTCAACCTTTGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00902 ko01110 map00902 map01110
Pfam Domains
Protein Families
adh_short adh_short_C2

Protein Analysis

298

Amino Acids

32.0

Weight (kDa)

8.56

Isoelectric Point (pI)

25.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
adh_short PF00106 18 - 173 8.8e-29 short chain dehydrogenase
KR PF08659 19 - 111 7.3e-08 KR domain
adh_short_C2 PF13561 23 - 170 5.7e-19 Enoyl-(Acyl carrier protein) reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 341, 590
AccB7I CCANNNNNTGG 1 cut(s) 642
AccI GTMKAC 1 cut(s) 324
AciI CCGC 4 cut(s) 22, 392, 395, 859
AclWI GGATC 4 cut(s) 13, 36, 49, 325
AcsI RAATTY 2 cut(s) 599, 849
AcuI CTGAAG 1 cut(s) 489
AfiI CCNNNNNNNGG 3 cut(s) 78, 571, 642
AgsI TTSAA 4 cut(s) 406, 461, 525, 800
AjnI CCWGG 1 cut(s) 748
Alw26I GTCTC 3 cut(s) 380, 496, 508
AlwI GGATC 4 cut(s) 13, 36, 49, 325
AoxI GGCC 3 cut(s) 347, 644, 856
ApeKI GCWGC 3 cut(s) 167, 680, 806
ApoI RAATTY 2 cut(s) 599, 849
AspS9I GGNCC 3 cut(s) 347, 746, 815
AsuC2I CCSGG 1 cut(s) 784
AsuHPI GGTGA 1 cut(s) 559
AvaII GGWCC 2 cut(s) 746, 815
BamHI GGATCC 1 cut(s) 41
BauI CACGAG 1 cut(s) 34
BbsI GAAGAC 1 cut(s) 199
BbvI GCAGC 3 cut(s) 179, 692, 793
BccI CCATC 2 cut(s) 219, 636
BciT130I CCWGG 1 cut(s) 750
BciVI GTATCC 1 cut(s) 729
BcnI CCSGG 1 cut(s) 784
BcoDI GTCTC 3 cut(s) 380, 496, 508
BfaI CTAG 6 cut(s) 218, 237, 548, 590, 605, 812
BfuI GTATCC 1 cut(s) 729
BglII AGATCT 1 cut(s) 766
BisI GCNGC 5 cut(s) 168, 393, 681, 807, 859
BlsI GCNGC 5 cut(s) 169, 394, 682, 808, 860
Bme1390I CCNGG 2 cut(s) 750, 784
Bme18I GGWCC 2 cut(s) 746, 815
BmgT120I GGNCC 3 cut(s) 347, 746, 815
BmiI GGNNCC 2 cut(s) 43, 70
BmrFI CCNGG 2 cut(s) 750, 784
BmsI GCATC 1 cut(s) 136
BpiI GAAGAC 1 cut(s) 199
BpmI CTGGAG 1 cut(s) 87
BpuEI CTTGAG 1 cut(s) 110
BpuMI CCSGG 1 cut(s) 784
BsaJI CCNNGG 2 cut(s) 313, 570
BsaWI WCCGGW 1 cut(s) 870
BsaXI ACNNNNNCTCC 2 cut(s) 111, 141
Bsc4I CCNNNNNNNGG 3 cut(s) 78, 571, 642
Bse1I ACTGG 2 cut(s) 70, 850
Bse3DI GCAATG 2 cut(s) 240, 726
BseBI CCWGG 1 cut(s) 750
BseDI CCNNGG 2 cut(s) 313, 570
BseGI GGATG 2 cut(s) 616, 647
BseLI CCNNNNNNNGG 3 cut(s) 78, 571, 642
BseMI GCAATG 2 cut(s) 240, 726
BseMII CTCAG 1 cut(s) 503
BseNI ACTGG 2 cut(s) 70, 850
BseRI GAGGAG 1 cut(s) 587
BseXI GCAGC 3 cut(s) 179, 692, 793
BsgI GTGCAG 1 cut(s) 792
BshFI GGCC 3 cut(s) 349, 646, 858
BsiSI CCGG 2 cut(s) 783, 871
BslFI GGGAC 1 cut(s) 519
BslI CCNNNNNNNGG 3 cut(s) 78, 571, 642
BsmAI GTCTC 3 cut(s) 380, 496, 508
BsmFI GGGAC 1 cut(s) 519
BsnI GGCC 3 cut(s) 349, 646, 858
Bsp143I GATC 4 cut(s) 18, 41, 317, 766
BspACI CCGC 4 cut(s) 22, 392, 395, 859
BspANI GGCC 3 cut(s) 349, 646, 858
BspCNI CTCAG 1 cut(s) 504
BspLI GGNNCC 2 cut(s) 43, 70
BspPI GGATC 4 cut(s) 13, 36, 49, 325
BsrDI GCAATG 2 cut(s) 240, 726
BsrI ACTGG 2 cut(s) 70, 850
BssECI CCNNGG 2 cut(s) 313, 570
BssMI GATC 4 cut(s) 18, 41, 317, 766
BssNAI GTATAC 1 cut(s) 325
BssSI CACGAG 1 cut(s) 34
BssT1I CCWWGG 1 cut(s) 313
Bst1107I GTATAC 1 cut(s) 325
Bst2BI CACGAG 1 cut(s) 34
Bst2UI CCWGG 1 cut(s) 750
Bst4CI ACNGT 1 cut(s) 796
Bst6I CTCTTC 3 cut(s) 146, 499, 873
BstAPI GCANNNNNTGC 2 cut(s) 30, 146
BstC8I GCNNGC 1 cut(s) 828
BstDEI CTNAG 1 cut(s) 512
BstENI CCTNNNNNAGG 1 cut(s) 569
BstF5I GGATG 2 cut(s) 616, 647
BstKTI GATC 4 cut(s) 21, 44, 320, 769
BstMAI GTCTC 3 cut(s) 380, 496, 508
BstMBI GATC 4 cut(s) 18, 41, 317, 766
BstMWI GCNNNNNNNGC 7 cut(s) 21, 30, 146, 251, 689, 737, 836
BstNI CCWGG 1 cut(s) 750
BstSCI CCNGG 2 cut(s) 748, 782
BstV1I GCAGC 3 cut(s) 179, 692, 793
BstV2I GAAGAC 1 cut(s) 199
BstX2I RGATCY 3 cut(s) 41, 317, 766
BstYI RGATCY 3 cut(s) 41, 317, 766
BstZ17I GTATAC 1 cut(s) 325
BsuI GTATCC 1 cut(s) 729
BsuRI GGCC 3 cut(s) 349, 646, 858
BtsCI GGATG 2 cut(s) 616, 647
Cac8I GCNNGC 1 cut(s) 828
Cfr13I GGNCC 3 cut(s) 347, 746, 815
CspCI CAANNNNNGTGG 4 cut(s) 34, 69, 687, 722
CviAII CATG 2 cut(s) 30, 620
DdeI CTNAG 1 cut(s) 512
DpnI GATC 4 cut(s) 20, 43, 319, 768
DpnII GATC 4 cut(s) 18, 41, 317, 766
DrdI GACNNNNNNGTC 2 cut(s) 341, 590
DseDI GACNNNNNNGTC 2 cut(s) 341, 590
Eam1104I CTCTTC 3 cut(s) 146, 499, 873
EarI CTCTTC 3 cut(s) 146, 499, 873
EciI GGCGGA 1 cut(s) 410
Eco130I CCWWGG 1 cut(s) 313
Eco47I GGWCC 2 cut(s) 746, 815
Eco57I CTGAAG 1 cut(s) 489
EcoNI CCTNNNNNAGG 1 cut(s) 569
EcoO109I RGGNCCY 1 cut(s) 815
EcoRII CCWGG 1 cut(s) 748
EcoT14I CCWWGG 1 cut(s) 313
ErhI CCWWGG 1 cut(s) 313
FaeI CATG 2 cut(s) 33, 623
FalI AAGNNNNNCTT 2 cut(s) 669, 701
FaqI GGGAC 1 cut(s) 519
FatI CATG 2 cut(s) 29, 619
FblI GTMKAC 1 cut(s) 324
Fnu4HI GCNGC 5 cut(s) 168, 393, 681, 807, 859
FokI GGATG 2 cut(s) 623, 654
Fsp4HI GCNGC 5 cut(s) 168, 393, 681, 807, 859
FspBI CTAG 6 cut(s) 218, 237, 548, 590, 605, 812
GluI GCNGC 5 cut(s) 168, 393, 681, 807, 859
GsuI CTGGAG 1 cut(s) 87
HaeIII GGCC 3 cut(s) 349, 646, 858
HapII CCGG 2 cut(s) 783, 871
Hin1II CATG 2 cut(s) 33, 623
HincII GTYRAC 2 cut(s) 298, 885
HindII GTYRAC 2 cut(s) 298, 885
HindIII AAGCTT 1 cut(s) 281
HinfI GANTC 6 cut(s) 309, 423, 623, 702, 787, 881
HpaI GTTAAC 1 cut(s) 298
HpaII CCGG 2 cut(s) 783, 871
HphI GGTGA 1 cut(s) 559
Hpy166II GTNNAC 4 cut(s) 298, 325, 583, 885
Hpy188I TCNGA 3 cut(s) 199, 278, 469
Hpy188III TCNNGA 1 cut(s) 605
Hpy8I GTNNAC 4 cut(s) 298, 325, 583, 885
HpyAV CCTTC 1 cut(s) 535
HpyCH4III ACNGT 1 cut(s) 796
HpyCH4V TGCA 5 cut(s) 33, 56, 305, 376, 809
HpyF10VI GCNNNNNNNGC 7 cut(s) 21, 30, 146, 251, 689, 737, 836
HpyF3I CTNAG 1 cut(s) 512
Hsp92II CATG 2 cut(s) 33, 623
KspAI GTTAAC 1 cut(s) 298
Kzo9I GATC 4 cut(s) 18, 41, 317, 766
LmnI GCTCC 3 cut(s) 68, 478, 544
Lsp1109I GCAGC 3 cut(s) 179, 692, 793
LweI GCATC 1 cut(s) 136
MaeI CTAG 6 cut(s) 218, 237, 548, 590, 605, 812
MaeIII GTNAC 2 cut(s) 61, 223
MalI GATC 4 cut(s) 20, 43, 319, 768
MboI GATC 4 cut(s) 18, 41, 317, 766
MboII GAAGA 5 cut(s) 163, 199, 486, 812, 890
MflI RGATCY 3 cut(s) 41, 317, 766
MluCI AATT 4 cut(s) 104, 272, 599, 849
MlyI GAGTC 3 cut(s) 318, 711, 890
MnlI CCTC 7 cut(s) 149, 338, 508, 565, 575, 589, 601
MseI TTAA 5 cut(s) 134, 297, 360, 687, 867
MspA1I CMGCKG 3 cut(s) 170, 392, 511
MspI CCGG 2 cut(s) 783, 871
MspR9I CCNGG 2 cut(s) 750, 784
MvaI CCWGG 1 cut(s) 750
MwoI GCNNNNNNNGC 7 cut(s) 21, 30, 146, 251, 689, 737, 836
NciI CCSGG 1 cut(s) 784
NdeII GATC 4 cut(s) 18, 41, 317, 766
NlaIII CATG 2 cut(s) 33, 623
NlaIV GGNNCC 2 cut(s) 43, 70
PfeI GAWTC 3 cut(s) 423, 623, 787
PflFI GACNNNGTC 1 cut(s) 341
PflMI CCANNNNNTGG 1 cut(s) 642
PkrI GCNGC 5 cut(s) 169, 394, 682, 808, 860
PleI GAGTC 3 cut(s) 317, 710, 889
PpsI GAGTC 3 cut(s) 317, 710, 889
PpuMI RGGWCCY 1 cut(s) 815
Psp5II RGGWCCY 1 cut(s) 815
Psp6I CCWGG 1 cut(s) 748
PspGI CCWGG 1 cut(s) 748
PspN4I GGNNCC 2 cut(s) 43, 70
PspPI GGNCC 3 cut(s) 347, 746, 815
PspPPI RGGWCCY 1 cut(s) 815
PsuI RGATCY 3 cut(s) 41, 317, 766
PsyI GACNNNGTC 1 cut(s) 341
PvuII CAGCTG 2 cut(s) 170, 511
SaqAI TTAA 5 cut(s) 134, 297, 360, 687, 867
SatI GCNGC 5 cut(s) 168, 393, 681, 807, 859
Sau3AI GATC 4 cut(s) 18, 41, 317, 766
Sau96I GGNCC 3 cut(s) 347, 746, 815
SchI GAGTC 3 cut(s) 318, 711, 890
ScrFI CCNGG 2 cut(s) 750, 784
SfaNI GCATC 1 cut(s) 136
SinI GGWCC 2 cut(s) 746, 815
SmlI CTYRAG 1 cut(s) 89
SmoI CTYRAG 1 cut(s) 89
Sse9I AATT 4 cut(s) 104, 272, 599, 849
SsiI CCGC 4 cut(s) 22, 392, 395, 859
SspMI CTAG 6 cut(s) 218, 237, 548, 590, 605, 812
StyD4I CCNGG 2 cut(s) 748, 782
StyI CCWWGG 1 cut(s) 313
TaaI ACNGT 1 cut(s) 796
TaqI TCGA 2 cut(s) 594, 626
TasI AATT 4 cut(s) 104, 272, 599, 849
TauI GCSGC 2 cut(s) 395, 861
TfiI GAWTC 3 cut(s) 423, 623, 787
Tru1I TTAA 5 cut(s) 134, 297, 360, 687, 867
Tru9I TTAA 5 cut(s) 134, 297, 360, 687, 867
TseI GCWGC 3 cut(s) 167, 680, 806
TspDTI ATGAA 1 cut(s) 891
Tth111I GACNNNGTC 1 cut(s) 341
Van91I CCANNNNNTGG 1 cut(s) 642
VpaK11BI GGWCC 2 cut(s) 746, 815
XagI CCTNNNNNAGG 1 cut(s) 569
XapI RAATTY 2 cut(s) 599, 849
XbaI TCTAGA 1 cut(s) 604
XmiI GTMKAC 1 cut(s) 324
XspI CTAG 6 cut(s) 218, 237, 548, 590, 605, 812
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.