RLG00000003862

Salutaridine reductase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0001077
Physical Location & Seq
Forward (+)
53664003 .. 53667756
3754 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000003862

Sequence Viewer

Length: 630 bp
ATGACCAAGACAGCAAAAGAAGAAGAAGCAAGCTTGAATGTCGAAGTAGCATCATCGGAGATTAAGGAGCAGTCCGGGCATTCCCACAGCGTCGTCGAGGCCAGGCAGCATGCCACGATAAAGCTTCTCAATCGAAACAAAATGAGAAAAACTTCAACCACCTCATGCAGTCCCGACCTCACCTTACCTCTGCCCAATAACTCCAGTCGGTTCTCTTCTGATCACCGCTGCAAATCTGTGATCCACCCTCAGCTTATTTCAAATGAGAAAGCAAAGAAGGAGCTAGAAGATGTGGATAACCTCACCGAGGAGAAAGTGGACAAGCTAGTTGAGGAATTTCTAGAGGATGTGAAGCGGGATTTGATAGAATCTAAAGGCTGGCCTTTAGATATATCTACTTACATTGTATCACAAGCTCTTAATGCTTATACAAGAGTCGTGGCAAAGAAACATCCCCAAATTGCTATAAACGCAGTTAGTCCAGGTTATACTAAAACTGATCTCTACAATAATACCGGGATTCTCACAATTGAAGAAGCTGCACTAGTTCCTGTGAAGCTGGCTTTGATAGCCAAAACTGGAATTTCAGGCCTCTTCTTTAACCAAACTGAAGAGTCAACCTTTGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00902 ko01110 map00902 map01110
Pfam Domains
Protein Families
adh_short adh_short_C2

Protein Analysis

210

Amino Acids

23.24

Weight (kDa)

5.91

Isoelectric Point (pI)

48.75

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 226, 355
AclWI GGATC 1 cut(s) 235
AcsI RAATTY 2 cut(s) 335, 582
AcuI CTGAAG 1 cut(s) 630
AfiI CCNNNNNNNGG 1 cut(s) 307
AgsI TTSAA 4 cut(s) 37, 156, 261, 533
AhlI ACTAGT 1 cut(s) 544
AjnI CCWGG 2 cut(s) 101, 481
AluBI AGCT 8 cut(s) 33, 124, 253, 283, 325, 416, 539, 559
AluI AGCT 8 cut(s) 33, 124, 253, 283, 325, 416, 539, 559
AlwI GGATC 1 cut(s) 235
AoxI GGCC 3 cut(s) 99, 380, 589
ApeKI GCWGC 3 cut(s) 106, 228, 539
ApoI RAATTY 2 cut(s) 335, 582
Asp700I GAANNNNTTC 1 cut(s) 151
AsuC2I CCSGG 2 cut(s) 76, 517
AsuHPI GGTGA 3 cut(s) 172, 215, 295
BbvCI CCTCAGC 1 cut(s) 249
BbvI GCAGC 3 cut(s) 118, 215, 526
BciT130I CCWGG 2 cut(s) 103, 483
BclI TGATCA 1 cut(s) 220
BcnI CCSGG 2 cut(s) 76, 517
BcuI ACTAGT 1 cut(s) 544
BfaI CTAG 4 cut(s) 284, 326, 341, 545
BisI GCNGC 3 cut(s) 107, 229, 540
BlsI GCNGC 3 cut(s) 108, 230, 541
Bme1390I CCNGG 4 cut(s) 76, 103, 483, 517
BmrFI CCNGG 4 cut(s) 76, 103, 483, 517
BmsI GCATC 1 cut(s) 59
BpmI CTGGAG 1 cut(s) 187
Bpu10I CCTNAGC 1 cut(s) 249
BpuMI CCSGG 2 cut(s) 76, 517
BsaJI CCNNGG 1 cut(s) 306
Bsc4I CCNNNNNNNGG 1 cut(s) 307
Bse1I ACTGG 2 cut(s) 204, 583
BseBI CCWGG 2 cut(s) 103, 483
BseDI CCNNGG 1 cut(s) 306
BseGI GGATG 2 cut(s) 352, 451
BseLI CCNNNNNNNGG 1 cut(s) 307
BseMII CTCAG 1 cut(s) 263
BseNI ACTGG 2 cut(s) 204, 583
BseRI GAGGAG 1 cut(s) 323
BseXI GCAGC 3 cut(s) 118, 215, 526
BsgI GTGCAG 1 cut(s) 525
BshFI GGCC 3 cut(s) 101, 382, 591
BsiSI CCGG 2 cut(s) 75, 516
BslFI GGGAC 1 cut(s) 156
BslI CCNNNNNNNGG 1 cut(s) 307
BsmFI GGGAC 1 cut(s) 156
BsmI GAATGC 1 cut(s) 79
BsnI GGCC 3 cut(s) 101, 382, 591
Bsp143I GATC 3 cut(s) 220, 240, 499
BspACI CCGC 2 cut(s) 226, 355
BspANI GGCC 3 cut(s) 101, 382, 591
BspCNI CTCAG 1 cut(s) 262
BspPI GGATC 1 cut(s) 235
BsrI ACTGG 2 cut(s) 204, 583
BssECI CCNNGG 1 cut(s) 306
BssMI GATC 3 cut(s) 220, 240, 499
Bst2UI CCWGG 2 cut(s) 103, 483
Bst6I CTCTTC 3 cut(s) 220, 599, 606
BstC8I GCNNGC 4 cut(s) 31, 111, 380, 561
BstDEI CTNAG 1 cut(s) 249
BstENI CCTNNNNNAGG 1 cut(s) 305
BstF5I GGATG 2 cut(s) 352, 451
BstKTI GATC 3 cut(s) 223, 243, 502
BstMBI GATC 3 cut(s) 220, 240, 499
BstMWI GCNNNNNNNGC 4 cut(s) 76, 422, 470, 569
BstNI CCWGG 2 cut(s) 103, 483
BstNSI RCATGY 1 cut(s) 113
BstSCI CCNGG 4 cut(s) 74, 101, 481, 515
BstV1I GCAGC 3 cut(s) 118, 215, 526
BsuRI GGCC 3 cut(s) 101, 382, 591
BtsCI GGATG 2 cut(s) 352, 451
Cac8I GCNNGC 4 cut(s) 31, 111, 380, 561
CseI GACGC 1 cut(s) 79
CspCI CAANNNNNGTGG 2 cut(s) 420, 455
CviAII CATG 2 cut(s) 110, 165
DdeI CTNAG 1 cut(s) 249
DpnI GATC 3 cut(s) 222, 242, 501
DpnII GATC 3 cut(s) 220, 240, 499
Eam1104I CTCTTC 3 cut(s) 220, 599, 606
EarI CTCTTC 3 cut(s) 220, 599, 606
Eco147I AGGCCT 1 cut(s) 591
Eco57I CTGAAG 1 cut(s) 630
EcoNI CCTNNNNNAGG 1 cut(s) 305
EcoRII CCWGG 2 cut(s) 101, 481
FaeI CATG 2 cut(s) 113, 168
FaiI YATR 6 cut(s) 111, 166, 392, 429, 467, 489
FaqI GGGAC 1 cut(s) 156
FatI CATG 2 cut(s) 109, 164
FauI CCCGC 1 cut(s) 348
FbaI TGATCA 1 cut(s) 220
Fnu4HI GCNGC 3 cut(s) 107, 229, 540
FokI GGATG 2 cut(s) 359, 438
Fsp4HI GCNGC 3 cut(s) 107, 229, 540
FspBI CTAG 4 cut(s) 284, 326, 341, 545
GluI GCNGC 3 cut(s) 107, 229, 540
GsuI CTGGAG 1 cut(s) 187
HaeIII GGCC 3 cut(s) 101, 382, 591
HapII CCGG 2 cut(s) 75, 516
HgaI GACGC 1 cut(s) 79
Hin1II CATG 2 cut(s) 113, 168
HincII GTYRAC 1 cut(s) 618
HindII GTYRAC 1 cut(s) 618
HindIII AAGCTT 2 cut(s) 31, 122
HinfI GANTC 4 cut(s) 368, 435, 520, 614
HpaII CCGG 2 cut(s) 75, 516
HphI GGTGA 3 cut(s) 172, 215, 295
Hpy166II GTNNAC 2 cut(s) 319, 618
Hpy188I TCNGA 2 cut(s) 58, 220
Hpy188III TCNNGA 2 cut(s) 173, 341
Hpy8I GTNNAC 2 cut(s) 319, 618
Hpy99I CGWCG 2 cut(s) 95, 98
HpyAV CCTTC 1 cut(s) 271
HpyCH4V TGCA 3 cut(s) 168, 231, 542
HpyF10VI GCNNNNNNNGC 4 cut(s) 76, 422, 470, 569
HpyF3I CTNAG 1 cut(s) 249
Hsp92II CATG 2 cut(s) 113, 168
Ksp22I TGATCA 1 cut(s) 220
Kzo9I GATC 3 cut(s) 220, 240, 499
LmnI GCTCC 2 cut(s) 67, 280
Lsp1109I GCAGC 3 cut(s) 118, 215, 526
LweI GCATC 1 cut(s) 59
MaeI CTAG 4 cut(s) 284, 326, 341, 545
MalI GATC 3 cut(s) 222, 242, 501
MboI GATC 3 cut(s) 220, 240, 499
MboII GAAGA 7 cut(s) 32, 35, 207, 299, 545, 586, 623
MfeI CAATTG 1 cut(s) 528
MluCI AATT 4 cut(s) 335, 459, 528, 582
MlyI GAGTC 2 cut(s) 444, 623
MroXI GAANNNNTTC 1 cut(s) 151
MseI TTAA 3 cut(s) 63, 420, 600
MspA1I CMGCKG 1 cut(s) 228
MspI CCGG 2 cut(s) 75, 516
MspR9I CCNGG 4 cut(s) 76, 103, 483, 517
MunI CAATTG 1 cut(s) 528
Mva1269I GAATGC 1 cut(s) 79
MvaI CCWGG 2 cut(s) 103, 483
MwoI GCNNNNNNNGC 4 cut(s) 76, 422, 470, 569
NciI CCSGG 2 cut(s) 76, 517
NdeII GATC 3 cut(s) 220, 240, 499
NlaIII CATG 2 cut(s) 113, 168
NspI RCATGY 1 cut(s) 113
PaeI GCATGC 1 cut(s) 113
PceI AGGCCT 1 cut(s) 591
PctI GAATGC 1 cut(s) 79
PdmI GAANNNNTTC 1 cut(s) 151
PfeI GAWTC 2 cut(s) 368, 520
PkrI GCNGC 3 cut(s) 108, 230, 541
PleI GAGTC 2 cut(s) 443, 622
PpsI GAGTC 2 cut(s) 443, 622
Psp6I CCWGG 2 cut(s) 101, 481
PspGI CCWGG 2 cut(s) 101, 481
SaqAI TTAA 3 cut(s) 63, 420, 600
SatI GCNGC 3 cut(s) 107, 229, 540
Sau3AI GATC 3 cut(s) 220, 240, 499
SchI GAGTC 2 cut(s) 444, 623
ScrFI CCNGG 4 cut(s) 76, 103, 483, 517
SfaNI GCATC 1 cut(s) 59
SpeI ACTAGT 1 cut(s) 544
SphI GCATGC 1 cut(s) 113
Sse9I AATT 4 cut(s) 335, 459, 528, 582
SseBI AGGCCT 1 cut(s) 591
SsiI CCGC 2 cut(s) 226, 355
SspMI CTAG 4 cut(s) 284, 326, 341, 545
StuI AGGCCT 1 cut(s) 591
StyD4I CCNGG 4 cut(s) 74, 101, 481, 515
TaqI TCGA 3 cut(s) 42, 96, 133
TasI AATT 4 cut(s) 335, 459, 528, 582
TfiI GAWTC 2 cut(s) 368, 520
Tru1I TTAA 3 cut(s) 63, 420, 600
Tru9I TTAA 3 cut(s) 63, 420, 600
TseI GCWGC 3 cut(s) 106, 228, 539
XagI CCTNNNNNAGG 1 cut(s) 305
XapI RAATTY 2 cut(s) 335, 582
XbaI TCTAGA 1 cut(s) 340
XceI RCATGY 1 cut(s) 113
XmnI GAANNNNTTC 1 cut(s) 151
XspI CTAG 4 cut(s) 284, 326, 341, 545
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.