Rw7G015060

Salutaridine reductase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0001077
Physical Location & Seq
Reverse (-)
14965836 .. 14969146
3311 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G015060.1

Sequence Viewer

Length: 927 bp
ATGCAATTGCTGGAAATAATCTTGAGTTCAGACAAAAAAAGAAAAGAATATAGAAGACATCTTGAATATTCTTTCATCCTTATTCATATTGTTACTGGAGCCAACAAAGGGATTGGACTTGAGATTAGTAAGCAATTAGCTTCTAAAGGAGTTGGGGTGGTATTAACAGCAAGAGATGCGAAGAGAGGAACAGAAGCAGCTGAAAGCCTAAAGGCTTCTGGTTTCTCCGATGTGGTCTTCCATCAGCTAGACGTAACTGCCCCAACTAGCATTGCTTCTTTGGCAAACTTTCTCAAAACGCAATTCAGAAAGCTTGACATATTGGTTAACAACGCAGGAGTCCTTGGATCTGTATTCCTCGCAGACGACATAGTTGGGCCTGACAATGTTAAACAAAATACAGTGCAAACTTACGAGACAGCGGCGGATTGTTTGAAAACAAACTATTATGGAATCAAGCAACTCACAGAAGCACTTGTTCCACTACTTCAAAAATCTGAAGCTCCAAAGATAGTCAATGTCTCTTCCACACTGGGACAGCTAAGACTTATTTCAAATGAGAAAGCAAAGAAGGAGCTAGGAGATGCGGATAACCTCACCGAGGAGAAAGTGGACAAGCTAGTTGAGGAATTTCTAGAGGATGTGAAGCATGATTCGATAGAATCCAAAGGATGGCCTTTAGATATATCTGCCTACATTGTATCGAAAGCAGCTCTTAATGCTTATACAAGAGTCGTGGCAAAGAAGTATCCCCACATTGCTATTAACGCAGTTAGTCCAGGTTATACCAAAACAGATCTCAACAATAATACTGGGATTCTCACAGTTGAAGAAGCTGCACTTGGTCCTGTGAAGCTGGCTTTGATAGCCGAAACTGGAATTTCAGGCCTCTTCTTTGACCGGACTGAAGAGTCAACCTTTGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00902 ko01110 map00902 map01110
Pfam Domains
Protein Families
adh_short adh_short_C2

Protein Analysis

308

Amino Acids

33.6

Weight (kDa)

5.85

Isoelectric Point (pI)

31.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KR PF08659 29 - 121 4e-08 KR domain
adh_short PF00106 30 - 183 1.2e-27 short chain dehydrogenase
adh_short_C2 PF13561 33 - 180 5.6e-19 Enoyl-(Acyl carrier protein) reductase
adh_short_C2 PF13561 228 - 270 1.5e-06 Enoyl-(Acyl carrier protein) reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 910
AccB7I CCANNNNNTGG 1 cut(s) 672
AciI CCGC 3 cut(s) 422, 425, 587
AclWI GGATC 1 cut(s) 355
AcsI RAATTY 2 cut(s) 629, 879
AcuI CTGAAG 2 cut(s) 519, 927
AfiI CCNNNNNNNGG 3 cut(s) 108, 601, 672
AgsI TTSAA 5 cut(s) 65, 436, 491, 555, 830
AjnI CCWGG 1 cut(s) 778
AjuI GAANNNNNNNTTGG 2 cut(s) 825, 857
Alw26I GTCTC 2 cut(s) 410, 526
AlwI GGATC 1 cut(s) 355
AoxI GGCC 3 cut(s) 377, 674, 886
ApeKI GCWGC 3 cut(s) 197, 710, 836
ApoI RAATTY 2 cut(s) 629, 879
AspS9I GGNCC 2 cut(s) 377, 845
AsuHPI GGTGA 1 cut(s) 589
AvaII GGWCC 1 cut(s) 845
BbsI GAAGAC 2 cut(s) 61, 229
BbvI GCAGC 3 cut(s) 209, 722, 823
BccI CCATC 2 cut(s) 249, 666
BciT130I CCWGG 1 cut(s) 780
BciVI GTATCC 1 cut(s) 759
BcoDI GTCTC 2 cut(s) 410, 526
BfaI CTAG 5 cut(s) 248, 267, 578, 620, 635
BfuI GTATCC 1 cut(s) 759
BglII AGATCT 1 cut(s) 796
BisI GCNGC 4 cut(s) 198, 423, 711, 837
BlsI GCNGC 4 cut(s) 199, 424, 712, 838
Bme1390I CCNGG 1 cut(s) 780
Bme18I GGWCC 1 cut(s) 845
BmgT120I GGNCC 2 cut(s) 377, 845
BmiI GGNNCC 1 cut(s) 100
BmrFI CCNGG 1 cut(s) 780
BmrI ACTGGG 2 cut(s) 542, 822
BmsI GCATC 2 cut(s) 166, 574
BmuI ACTGGG 2 cut(s) 542, 822
BpiI GAAGAC 2 cut(s) 61, 229
BpmI CTGGAG 1 cut(s) 117
BpuEI CTTGAG 2 cut(s) 43, 140
BsaJI CCNNGG 2 cut(s) 343, 600
BsaWI WCCGGW 1 cut(s) 900
BsaXI ACNNNNNCTCC 2 cut(s) 141, 171
Bsc4I CCNNNNNNNGG 3 cut(s) 108, 601, 672
Bse1I ACTGG 4 cut(s) 100, 537, 817, 880
Bse3DI GCAATG 2 cut(s) 270, 756
BseBI CCWGG 1 cut(s) 780
BseDI CCNNGG 2 cut(s) 343, 600
BseGI GGATG 3 cut(s) 75, 646, 677
BseLI CCNNNNNNNGG 3 cut(s) 108, 601, 672
BseMI GCAATG 2 cut(s) 270, 756
BseNI ACTGG 4 cut(s) 100, 537, 817, 880
BseRI GAGGAG 1 cut(s) 617
BseXI GCAGC 3 cut(s) 209, 722, 823
BsgI GTGCAG 1 cut(s) 822
BshFI GGCC 3 cut(s) 379, 676, 888
BsiSI CCGG 1 cut(s) 901
BslFI GGGAC 1 cut(s) 549
BslI CCNNNNNNNGG 3 cut(s) 108, 601, 672
BsmAI GTCTC 2 cut(s) 410, 526
BsmFI GGGAC 1 cut(s) 549
BsnI GGCC 3 cut(s) 379, 676, 888
Bsp143I GATC 2 cut(s) 347, 796
BspACI CCGC 3 cut(s) 422, 425, 587
BspANI GGCC 3 cut(s) 379, 676, 888
BspLI GGNNCC 1 cut(s) 100
BspPI GGATC 1 cut(s) 355
BsrDI GCAATG 2 cut(s) 270, 756
BsrI ACTGG 4 cut(s) 100, 537, 817, 880
BssECI CCNNGG 2 cut(s) 343, 600
BssMI GATC 2 cut(s) 347, 796
BssT1I CCWWGG 1 cut(s) 343
Bst2UI CCWGG 1 cut(s) 780
Bst4CI ACNGT 2 cut(s) 403, 826
Bst6I CTCTTC 4 cut(s) 176, 529, 896, 903
BstAPI GCANNNNNTGC 1 cut(s) 176
BstC8I GCNNGC 1 cut(s) 858
BstDEI CTNAG 1 cut(s) 542
BstENI CCTNNNNNAGG 1 cut(s) 599
BstF5I GGATG 3 cut(s) 75, 646, 677
BstKTI GATC 2 cut(s) 350, 799
BstMAI GTCTC 2 cut(s) 410, 526
BstMBI GATC 2 cut(s) 347, 796
BstMWI GCNNNNNNNGC 5 cut(s) 176, 281, 719, 767, 866
BstNI CCWGG 1 cut(s) 780
BstSCI CCNGG 1 cut(s) 778
BstV1I GCAGC 3 cut(s) 209, 722, 823
BstV2I GAAGAC 2 cut(s) 61, 229
BstX2I RGATCY 2 cut(s) 347, 796
BstYI RGATCY 2 cut(s) 347, 796
BsuI GTATCC 1 cut(s) 759
BsuRI GGCC 3 cut(s) 379, 676, 888
BtsCI GGATG 3 cut(s) 75, 646, 677
BtsIMutI CAGTG 2 cut(s) 408, 530
Cac8I GCNNGC 1 cut(s) 858
Cfr13I GGNCC 2 cut(s) 377, 845
CspCI CAANNNNNGTGG 2 cut(s) 717, 752
CviAII CATG 1 cut(s) 650
DdeI CTNAG 1 cut(s) 542
DpnI GATC 2 cut(s) 349, 798
DpnII GATC 2 cut(s) 347, 796
DrdI GACNNNNNNGTC 1 cut(s) 910
DseDI GACNNNNNNGTC 1 cut(s) 910
Eam1104I CTCTTC 4 cut(s) 176, 529, 896, 903
EarI CTCTTC 4 cut(s) 176, 529, 896, 903
EciI GGCGGA 1 cut(s) 440
Eco130I CCWWGG 1 cut(s) 343
Eco147I AGGCCT 1 cut(s) 888
Eco47I GGWCC 1 cut(s) 845
Eco57I CTGAAG 2 cut(s) 519, 927
EcoNI CCTNNNNNAGG 1 cut(s) 599
EcoRII CCWGG 1 cut(s) 778
EcoT14I CCWWGG 1 cut(s) 343
ErhI CCWWGG 1 cut(s) 343
FaeI CATG 1 cut(s) 653
FaiI YATR 9 cut(s) 51, 87, 320, 371, 450, 651, 686, 726, 786
FalI AAGNNNNNCTT 4 cut(s) 699, 731, 825, 857
FaqI GGGAC 1 cut(s) 549
FatI CATG 1 cut(s) 649
Fnu4HI GCNGC 4 cut(s) 198, 423, 711, 837
FokI GGATG 3 cut(s) 62, 653, 684
Fsp4HI GCNGC 4 cut(s) 198, 423, 711, 837
FspBI CTAG 5 cut(s) 248, 267, 578, 620, 635
GluI GCNGC 4 cut(s) 198, 423, 711, 837
GsuI CTGGAG 1 cut(s) 117
HaeIII GGCC 3 cut(s) 379, 676, 888
HapII CCGG 1 cut(s) 901
Hin1II CATG 1 cut(s) 653
HincII GTYRAC 2 cut(s) 328, 915
HindII GTYRAC 2 cut(s) 328, 915
HindIII AAGCTT 1 cut(s) 311
HinfI GANTC 7 cut(s) 339, 453, 653, 662, 732, 817, 911
HpaI GTTAAC 1 cut(s) 328
HpaII CCGG 1 cut(s) 901
HphI GGTGA 1 cut(s) 589
Hpy166II GTNNAC 3 cut(s) 328, 613, 915
Hpy188I TCNGA 4 cut(s) 31, 229, 308, 499
Hpy188III TCNNGA 3 cut(s) 22, 62, 635
Hpy8I GTNNAC 3 cut(s) 328, 613, 915
HpyAV CCTTC 1 cut(s) 565
HpyCH4III ACNGT 2 cut(s) 403, 826
HpyCH4IV ACGT 1 cut(s) 252
HpyCH4V TGCA 3 cut(s) 4, 406, 839
HpyF10VI GCNNNNNNNGC 5 cut(s) 176, 281, 719, 767, 866
HpyF3I CTNAG 1 cut(s) 542
HpySE526I ACGT 1 cut(s) 252
Hsp92II CATG 1 cut(s) 653
KspAI GTTAAC 1 cut(s) 328
Kzo9I GATC 2 cut(s) 347, 796
LmnI GCTCC 3 cut(s) 98, 508, 574
Lsp1109I GCAGC 3 cut(s) 209, 722, 823
LweI GCATC 2 cut(s) 166, 574
MaeI CTAG 5 cut(s) 248, 267, 578, 620, 635
MaeII ACGT 1 cut(s) 252
MaeIII GTNAC 2 cut(s) 91, 253
MalI GATC 2 cut(s) 349, 798
MboI GATC 2 cut(s) 347, 796
MboII GAAGA 7 cut(s) 66, 193, 229, 516, 842, 883, 920
MfeI CAATTG 1 cut(s) 5
MflI RGATCY 2 cut(s) 347, 796
MluCI AATT 5 cut(s) 5, 134, 302, 629, 879
MlyI GAGTC 3 cut(s) 348, 741, 920
MnlI CCTC 7 cut(s) 179, 368, 595, 605, 619, 631, 899
MseI TTAA 5 cut(s) 164, 327, 390, 717, 765
MspA1I CMGCKG 2 cut(s) 200, 422
MspI CCGG 1 cut(s) 901
MspR9I CCNGG 1 cut(s) 780
MunI CAATTG 1 cut(s) 5
MvaI CCWGG 1 cut(s) 780
MwoI GCNNNNNNNGC 5 cut(s) 176, 281, 719, 767, 866
NdeII GATC 2 cut(s) 347, 796
NlaIII CATG 1 cut(s) 653
NlaIV GGNNCC 1 cut(s) 100
PceI AGGCCT 1 cut(s) 888
PfeI GAWTC 4 cut(s) 453, 653, 662, 817
PflMI CCANNNNNTGG 1 cut(s) 672
PkrI GCNGC 4 cut(s) 199, 424, 712, 838
PleI GAGTC 3 cut(s) 347, 740, 919
PpsI GAGTC 3 cut(s) 347, 740, 919
Psp6I CCWGG 1 cut(s) 778
PspGI CCWGG 1 cut(s) 778
PspN4I GGNNCC 1 cut(s) 100
PspPI GGNCC 2 cut(s) 377, 845
PsuI RGATCY 2 cut(s) 347, 796
PvuII CAGCTG 1 cut(s) 200
SaqAI TTAA 5 cut(s) 164, 327, 390, 717, 765
SatI GCNGC 4 cut(s) 198, 423, 711, 837
Sau3AI GATC 2 cut(s) 347, 796
Sau96I GGNCC 2 cut(s) 377, 845
SchI GAGTC 3 cut(s) 348, 741, 920
ScrFI CCNGG 1 cut(s) 780
SfaNI GCATC 2 cut(s) 166, 574
SinI GGWCC 1 cut(s) 845
SmlI CTYRAG 2 cut(s) 22, 119
SmoI CTYRAG 2 cut(s) 22, 119
Sse9I AATT 5 cut(s) 5, 134, 302, 629, 879
SseBI AGGCCT 1 cut(s) 888
SsiI CCGC 3 cut(s) 422, 425, 587
SspI AATATT 1 cut(s) 68
SspMI CTAG 5 cut(s) 248, 267, 578, 620, 635
StuI AGGCCT 1 cut(s) 888
StyD4I CCNGG 1 cut(s) 778
StyI CCWWGG 1 cut(s) 343
TaaI ACNGT 2 cut(s) 403, 826
TaiI ACGT 1 cut(s) 255
TaqI TCGA 2 cut(s) 656, 704
TasI AATT 5 cut(s) 5, 134, 302, 629, 879
TauI GCSGC 1 cut(s) 425
TfiI GAWTC 4 cut(s) 453, 653, 662, 817
Tru1I TTAA 5 cut(s) 164, 327, 390, 717, 765
Tru9I TTAA 5 cut(s) 164, 327, 390, 717, 765
TscAI CASTG 2 cut(s) 408, 537
TseI GCWGC 3 cut(s) 197, 710, 836
TspDTI ATGAA 2 cut(s) 64, 74
TspRI CASTG 2 cut(s) 408, 537
Van91I CCANNNNNTGG 1 cut(s) 672
VpaK11BI GGWCC 1 cut(s) 845
XagI CCTNNNNNAGG 1 cut(s) 599
XapI RAATTY 2 cut(s) 629, 879
XbaI TCTAGA 1 cut(s) 634
XspI CTAG 5 cut(s) 248, 267, 578, 620, 635
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.