Rh7BG175000

Salutaridine reductase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0001077
Physical Location & Seq
Reverse (-)
13707682 .. 13711666
3985 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG175000.1

Sequence Viewer

Length: 900 bp
ATGTCCTCCGGCAGAAACAAGTCGGAAGGTGTTTTTTCTGAAGCATTTAGAAGGATTGCAGTTGTTACTGGAGCCAACAAAGGGATTGGACTTGAGATTAGTAAGCAATTAGCTTCTAAAGGAGTTGGGGTGGTATTAACAGCAAGAGATGCGAAGAGAGGAACAGAAGCAGCTGAAAGCCTAAAGGCTTCTAGTTTCTCCGATGTGGTCTTCCATCAGCTAGATGTAACTGCCCCAACTAGCATTGCTTCTTTGGCAAACTTTCTCAAAACGCAATTCAGAAAGCTTGACATATTGGTTAACAATGCAGGAGTCCTTGGATCTGTATACCTAGCAGACGACATAGTTGGGCCTGACAATGTTAAACAAAATACAGTGCAAACTTACGAGACAGCGGCGGATTGTTTGAAAACAAACTATTATGGAATCAAGCAACTCACAGAAGCACTTGTTCCACTACTTCAAAAATCTGAAGCTCCAAAGATAGTCAATGTCTCTTCCACAATGGGACAGCTAAGACTTATTTCAAATGAGAAAGCAAAGAAGGAGCTAGGAGATGCGGATAACCTCACCGAGGAGAAAGTGGACAAGCTAGTTGAGGAATTTCTAGAGGATGTGAAGCATGATTCGATAGAATCCAAAGGATGGCCTTTAGATATATCTGCCTACATTGTATCAAAAGCAGCTCTTAATGCTTATACAAGAGTCGTGGCAAAGAAGTATCCCCACATTGCTATTAACGCAGTTAGTCCAGGTTATACCAAAACAGATCTCAACAATAATACCGGGATTCTCACAGTTGAAGAAGCTGCACTTGGTCCTGTGAAGCTGGCTTTGATAGCCGAAACTGGAATTTCAGGCCTCTTCTTTGACCGGATTGAAGAGTCAACCTTTGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00902 ko01110 map00902 map01110
Pfam Domains
Protein Families
adh_short adh_short_C2

Protein Analysis

299

Amino Acids

32.23

Weight (kDa)

5.6

Isoelectric Point (pI)

33.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
adh_short PF00106 19 - 174 1.1e-28 short chain dehydrogenase
KR PF08659 21 - 112 2.3e-07 KR domain
adh_short_C2 PF13561 24 - 171 6.6e-19 Enoyl-(Acyl carrier protein) reductase
adh_short_C2 PF13561 219 - 261 1.4e-06 Enoyl-(Acyl carrier protein) reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 645
AccI GTMKAC 1 cut(s) 327
AciI CCGC 3 cut(s) 395, 398, 560
AclWI GGATC 1 cut(s) 328
AcsI RAATTY 2 cut(s) 602, 852
AcuI CTGAAG 2 cut(s) 60, 492
AfiI CCNNNNNNNGG 3 cut(s) 81, 574, 645
AgsI TTSAA 5 cut(s) 409, 464, 528, 803, 881
AjnI CCWGG 1 cut(s) 751
AjuI GAANNNNNNNTTGG 2 cut(s) 798, 830
Alw26I GTCTC 2 cut(s) 383, 499
AlwI GGATC 1 cut(s) 328
AoxI GGCC 3 cut(s) 350, 647, 859
ApeKI GCWGC 3 cut(s) 170, 683, 809
ApoI RAATTY 2 cut(s) 602, 852
AspS9I GGNCC 2 cut(s) 350, 818
AsuC2I CCSGG 1 cut(s) 787
AsuHPI GGTGA 1 cut(s) 562
AvaII GGWCC 1 cut(s) 818
BbsI GAAGAC 1 cut(s) 202
BbvI GCAGC 3 cut(s) 182, 695, 796
BccI CCATC 2 cut(s) 222, 639
BciT130I CCWGG 1 cut(s) 753
BciVI GTATCC 1 cut(s) 732
BcnI CCSGG 1 cut(s) 787
BcoDI GTCTC 2 cut(s) 383, 499
BfaI CTAG 7 cut(s) 192, 221, 240, 332, 551, 593, 608
BfuI GTATCC 1 cut(s) 732
BglII AGATCT 1 cut(s) 769
BisI GCNGC 4 cut(s) 171, 396, 684, 810
BlsI GCNGC 4 cut(s) 172, 397, 685, 811
Bme1390I CCNGG 2 cut(s) 753, 787
Bme18I GGWCC 1 cut(s) 818
BmgT120I GGNCC 2 cut(s) 350, 818
BmiI GGNNCC 1 cut(s) 73
BmrFI CCNGG 2 cut(s) 753, 787
BmsI GCATC 2 cut(s) 139, 547
BpiI GAAGAC 1 cut(s) 202
BpmI CTGGAG 1 cut(s) 90
BpuEI CTTGAG 1 cut(s) 113
BpuMI CCSGG 1 cut(s) 787
BsaJI CCNNGG 2 cut(s) 316, 573
BsaWI WCCGGW 1 cut(s) 873
BsaXI ACNNNNNCTCC 2 cut(s) 114, 144
Bsc4I CCNNNNNNNGG 3 cut(s) 81, 574, 645
Bse1I ACTGG 2 cut(s) 73, 853
Bse3DI GCAATG 2 cut(s) 243, 729
BseBI CCWGG 1 cut(s) 753
BseDI CCNNGG 2 cut(s) 316, 573
BseGI GGATG 2 cut(s) 619, 650
BseLI CCNNNNNNNGG 3 cut(s) 81, 574, 645
BseMI GCAATG 2 cut(s) 243, 729
BseNI ACTGG 2 cut(s) 73, 853
BseRI GAGGAG 1 cut(s) 590
BseXI GCAGC 3 cut(s) 182, 695, 796
BsgI GTGCAG 1 cut(s) 795
BshFI GGCC 3 cut(s) 352, 649, 861
BsiSI CCGG 3 cut(s) 9, 786, 874
BslFI GGGAC 1 cut(s) 522
BslI CCNNNNNNNGG 3 cut(s) 81, 574, 645
BsmAI GTCTC 2 cut(s) 383, 499
BsmFI GGGAC 1 cut(s) 522
BsnI GGCC 3 cut(s) 352, 649, 861
Bsp143I GATC 2 cut(s) 320, 769
BspACI CCGC 3 cut(s) 395, 398, 560
BspANI GGCC 3 cut(s) 352, 649, 861
BspLI GGNNCC 1 cut(s) 73
BspPI GGATC 1 cut(s) 328
BsrDI GCAATG 2 cut(s) 243, 729
BsrI ACTGG 2 cut(s) 73, 853
BssECI CCNNGG 2 cut(s) 316, 573
BssMI GATC 2 cut(s) 320, 769
BssNAI GTATAC 1 cut(s) 328
BssT1I CCWWGG 1 cut(s) 316
Bst1107I GTATAC 1 cut(s) 328
Bst2UI CCWGG 1 cut(s) 753
Bst4CI ACNGT 2 cut(s) 376, 799
Bst6I CTCTTC 4 cut(s) 149, 502, 869, 876
BstAPI GCANNNNNTGC 1 cut(s) 149
BstC8I GCNNGC 1 cut(s) 831
BstDEI CTNAG 1 cut(s) 515
BstENI CCTNNNNNAGG 1 cut(s) 572
BstF5I GGATG 2 cut(s) 619, 650
BstKTI GATC 2 cut(s) 323, 772
BstMAI GTCTC 2 cut(s) 383, 499
BstMBI GATC 2 cut(s) 320, 769
BstMWI GCNNNNNNNGC 5 cut(s) 149, 254, 692, 740, 839
BstNI CCWGG 1 cut(s) 753
BstSCI CCNGG 2 cut(s) 751, 785
BstV1I GCAGC 3 cut(s) 182, 695, 796
BstV2I GAAGAC 1 cut(s) 202
BstX2I RGATCY 2 cut(s) 320, 769
BstYI RGATCY 2 cut(s) 320, 769
BstZ17I GTATAC 1 cut(s) 328
BsuI GTATCC 1 cut(s) 732
BsuRI GGCC 3 cut(s) 352, 649, 861
BtsCI GGATG 2 cut(s) 619, 650
BtsIMutI CAGTG 1 cut(s) 381
Cac8I GCNNGC 1 cut(s) 831
Cfr13I GGNCC 2 cut(s) 350, 818
CspCI CAANNNNNGTGG 2 cut(s) 690, 725
CviAII CATG 1 cut(s) 623
DdeI CTNAG 1 cut(s) 515
DpnI GATC 2 cut(s) 322, 771
DpnII GATC 2 cut(s) 320, 769
Eam1104I CTCTTC 4 cut(s) 149, 502, 869, 876
EarI CTCTTC 4 cut(s) 149, 502, 869, 876
EciI GGCGGA 1 cut(s) 413
Eco130I CCWWGG 1 cut(s) 316
Eco147I AGGCCT 1 cut(s) 861
Eco47I GGWCC 1 cut(s) 818
Eco57I CTGAAG 2 cut(s) 60, 492
EcoNI CCTNNNNNAGG 1 cut(s) 572
EcoRII CCWGG 1 cut(s) 751
EcoT14I CCWWGG 1 cut(s) 316
ErhI CCWWGG 1 cut(s) 316
FaeI CATG 1 cut(s) 626
FaiI YATR 8 cut(s) 293, 328, 344, 423, 624, 659, 699, 759
FalI AAGNNNNNCTT 4 cut(s) 672, 704, 798, 830
FaqI GGGAC 1 cut(s) 522
FatI CATG 1 cut(s) 622
FblI GTMKAC 1 cut(s) 327
Fnu4HI GCNGC 4 cut(s) 171, 396, 684, 810
FokI GGATG 2 cut(s) 626, 657
Fsp4HI GCNGC 4 cut(s) 171, 396, 684, 810
FspBI CTAG 7 cut(s) 192, 221, 240, 332, 551, 593, 608
GluI GCNGC 4 cut(s) 171, 396, 684, 810
GsuI CTGGAG 1 cut(s) 90
HaeIII GGCC 3 cut(s) 352, 649, 861
HapII CCGG 3 cut(s) 9, 786, 874
Hin1II CATG 1 cut(s) 626
HincII GTYRAC 2 cut(s) 301, 888
HindII GTYRAC 2 cut(s) 301, 888
HindIII AAGCTT 1 cut(s) 284
HinfI GANTC 7 cut(s) 312, 426, 626, 635, 705, 790, 884
HpaI GTTAAC 1 cut(s) 301
HpaII CCGG 3 cut(s) 9, 786, 874
HphI GGTGA 1 cut(s) 562
Hpy166II GTNNAC 4 cut(s) 301, 328, 586, 888
Hpy188I TCNGA 5 cut(s) 25, 40, 202, 281, 472
Hpy188III TCNNGA 1 cut(s) 608
Hpy8I GTNNAC 4 cut(s) 301, 328, 586, 888
HpyAV CCTTC 3 cut(s) 20, 45, 538
HpyCH4III ACNGT 2 cut(s) 376, 799
HpyCH4V TGCA 4 cut(s) 59, 308, 379, 812
HpyF10VI GCNNNNNNNGC 5 cut(s) 149, 254, 692, 740, 839
HpyF3I CTNAG 1 cut(s) 515
Hsp92II CATG 1 cut(s) 626
KspAI GTTAAC 1 cut(s) 301
Kzo9I GATC 2 cut(s) 320, 769
LmnI GCTCC 3 cut(s) 71, 481, 547
Lsp1109I GCAGC 3 cut(s) 182, 695, 796
LweI GCATC 2 cut(s) 139, 547
MaeI CTAG 7 cut(s) 192, 221, 240, 332, 551, 593, 608
MaeIII GTNAC 2 cut(s) 64, 226
MalI GATC 2 cut(s) 322, 771
MboI GATC 2 cut(s) 320, 769
MboII GAAGA 6 cut(s) 166, 202, 489, 815, 856, 893
MflI RGATCY 2 cut(s) 320, 769
MluCI AATT 4 cut(s) 107, 275, 602, 852
MlyI GAGTC 3 cut(s) 321, 714, 893
MnlI CCTC 7 cut(s) 16, 152, 568, 578, 592, 604, 872
MseI TTAA 5 cut(s) 137, 300, 363, 690, 738
MspA1I CMGCKG 2 cut(s) 173, 395
MspI CCGG 3 cut(s) 9, 786, 874
MspR9I CCNGG 2 cut(s) 753, 787
MvaI CCWGG 1 cut(s) 753
MwoI GCNNNNNNNGC 5 cut(s) 149, 254, 692, 740, 839
NciI CCSGG 1 cut(s) 787
NdeII GATC 2 cut(s) 320, 769
NlaIII CATG 1 cut(s) 626
NlaIV GGNNCC 1 cut(s) 73
PceI AGGCCT 1 cut(s) 861
PfeI GAWTC 4 cut(s) 426, 626, 635, 790
PflMI CCANNNNNTGG 1 cut(s) 645
PkrI GCNGC 4 cut(s) 172, 397, 685, 811
PleI GAGTC 3 cut(s) 320, 713, 892
PpsI GAGTC 3 cut(s) 320, 713, 892
Psp6I CCWGG 1 cut(s) 751
PspGI CCWGG 1 cut(s) 751
PspN4I GGNNCC 1 cut(s) 73
PspPI GGNCC 2 cut(s) 350, 818
PsuI RGATCY 2 cut(s) 320, 769
PvuII CAGCTG 1 cut(s) 173
SaqAI TTAA 5 cut(s) 137, 300, 363, 690, 738
SatI GCNGC 4 cut(s) 171, 396, 684, 810
Sau3AI GATC 2 cut(s) 320, 769
Sau96I GGNCC 2 cut(s) 350, 818
SchI GAGTC 3 cut(s) 321, 714, 893
ScrFI CCNGG 2 cut(s) 753, 787
SfaNI GCATC 2 cut(s) 139, 547
SinI GGWCC 1 cut(s) 818
SmlI CTYRAG 1 cut(s) 92
SmoI CTYRAG 1 cut(s) 92
Sse9I AATT 4 cut(s) 107, 275, 602, 852
SseBI AGGCCT 1 cut(s) 861
SsiI CCGC 3 cut(s) 395, 398, 560
SspMI CTAG 7 cut(s) 192, 221, 240, 332, 551, 593, 608
StuI AGGCCT 1 cut(s) 861
StyD4I CCNGG 2 cut(s) 751, 785
StyI CCWWGG 1 cut(s) 316
TaaI ACNGT 2 cut(s) 376, 799
TaqI TCGA 1 cut(s) 629
TasI AATT 4 cut(s) 107, 275, 602, 852
TauI GCSGC 1 cut(s) 398
TfiI GAWTC 4 cut(s) 426, 626, 635, 790
Tru1I TTAA 5 cut(s) 137, 300, 363, 690, 738
Tru9I TTAA 5 cut(s) 137, 300, 363, 690, 738
TscAI CASTG 1 cut(s) 381
TseI GCWGC 3 cut(s) 170, 683, 809
TspRI CASTG 1 cut(s) 381
Van91I CCANNNNNTGG 1 cut(s) 645
VpaK11BI GGWCC 1 cut(s) 818
XagI CCTNNNNNAGG 1 cut(s) 572
XapI RAATTY 2 cut(s) 602, 852
XbaI TCTAGA 1 cut(s) 607
XmiI GTMKAC 1 cut(s) 327
XspI CTAG 7 cut(s) 192, 221, 240, 332, 551, 593, 608
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.