RchiOBHm_Chr7g0198451

Salutaridine reductase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0001077
Physical Location & Seq
Reverse (-)
16342933 .. 16345521
2589 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ17756

Sequence Viewer

Length: 882 bp
ATGGCGGAAACTACCATCAATTTTGGGTCAAAGAAGATTGCAGTTGTTACTGGAGCCAACAAAGGGATTGGACTTGAGATTAGTAAGCAATTAGCTTCTAAAGGAGTTGGGGTGGTATTAACAGCAAGAGATGCGAAGAGAGGAACAGAAGCAGCTGAAAGCCTAAAGGCTTCTGGTTTCTCCGATGTGGTCTTCCATCAGCTAGATGTAACTGCCCCAACTAGCATTGCTTCTTTGGCAAACTTTCTCAAAACGCAATTCAGAAAGCTTGACATATTGGTTAACAATGCAGGAGTCCTTGGATCTGTATACCTCGCAGACGACATAGTTGGGCCTAACAATATTAAACAAAATAAAGTGCAAAGTTACGAGACAGCGGTGGATTGTTTGAAAACAAACTATTATGGAATCAAGCAACTCACAGAAGCACTTGTTCCACTACTTCAAAAATCTGAAGCTCCAAAGATAGTCAATGTCTCTTCCTCACGGGGACAGCTGAGACTTATTTCAAATGAGAAAGCAAAGAAGGAGCTAGGAGATGTGGATAACCTCACCGAGGAGAAAGTGGACAAGCTAGTCGAGGAGTTTCTAGAGGATGTGAAGCATGATTCGATAAAATCCAAAGGATGGCCTTTAGATATATCTGCCTACATTGTATCAAAAGCAGCTCTTAATGCTTATACAAGAGTCATGGCAAAGAAGTATCCCCACATTGCTATAAACGCAGTTGGTCCAGGTTATACCAAAACAGATCTCAACAATAATACCGGGATTCTCACAGTTGAAGAAGCTGCACTAGATCCTGTGAAGCTGGCTTTGATAGCCGAAACTGGAATTTCAGGTCGCTTCTTTAACCGGAATGAAGAGTCAACCTTTGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00902 ko01110 map00902 map01110
Pfam Domains
Protein Families
adh_short adh_short_C2

Protein Analysis

293

Amino Acids

31.67

Weight (kDa)

7.71

Isoelectric Point (pI)

25.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
adh_short PF00106 12 - 168 1.3e-28 short chain dehydrogenase
KR PF08659 15 - 106 1.3e-07 KR domain
adh_short_C2 PF13561 18 - 165 1e-18 Enoyl-(Acyl carrier protein) reductase
adh_short_C2 PF13561 213 - 255 7.3e-06 Enoyl-(Acyl carrier protein) reductase
adh_short PF00106 214 - 255 6.7e-06 short chain dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 575
AccB7I CCANNNNNTGG 1 cut(s) 627
AccI GTMKAC 1 cut(s) 309
AciI CCGC 2 cut(s) 5, 377
AclWI GGATC 2 cut(s) 310, 794
AcsI RAATTY 1 cut(s) 834
AcuI CTGAAG 1 cut(s) 474
AfiI CCNNNNNNNGG 3 cut(s) 63, 556, 627
AgsI TTSAA 4 cut(s) 391, 446, 510, 785
AjnI CCWGG 1 cut(s) 733
Alw26I GTCTC 3 cut(s) 365, 481, 493
AlwI GGATC 2 cut(s) 310, 794
AoxI GGCC 2 cut(s) 332, 629
ApeKI GCWGC 3 cut(s) 152, 665, 791
ApoI RAATTY 1 cut(s) 834
AspS9I GGNCC 2 cut(s) 332, 731
AsuC2I CCSGG 1 cut(s) 769
AsuHPI GGTGA 1 cut(s) 544
AvaII GGWCC 1 cut(s) 731
BbsI GAAGAC 1 cut(s) 184
BbvI GCAGC 3 cut(s) 164, 677, 778
BccI CCATC 3 cut(s) 23, 204, 621
BciT130I CCWGG 1 cut(s) 735
BciVI GTATCC 1 cut(s) 714
BcnI CCSGG 1 cut(s) 769
BcoDI GTCTC 3 cut(s) 365, 481, 493
BfaI CTAG 6 cut(s) 203, 222, 533, 575, 590, 797
BfuI GTATCC 1 cut(s) 714
BglII AGATCT 1 cut(s) 751
BisI GCNGC 3 cut(s) 153, 666, 792
BlsI GCNGC 3 cut(s) 154, 667, 793
Bme1390I CCNGG 2 cut(s) 735, 769
Bme18I GGWCC 1 cut(s) 731
BmgT120I GGNCC 2 cut(s) 332, 731
BmiI GGNNCC 1 cut(s) 55
BmrFI CCNGG 2 cut(s) 735, 769
BmsI GCATC 1 cut(s) 121
BpiI GAAGAC 1 cut(s) 184
BpmI CTGGAG 1 cut(s) 72
BpuEI CTTGAG 1 cut(s) 95
BpuMI CCSGG 1 cut(s) 769
BsaJI CCNNGG 2 cut(s) 298, 555
BsaWI WCCGGW 1 cut(s) 855
BsaXI ACNNNNNCTCC 2 cut(s) 96, 126
Bsc4I CCNNNNNNNGG 3 cut(s) 63, 556, 627
Bse1I ACTGG 2 cut(s) 55, 835
Bse3DI GCAATG 2 cut(s) 225, 711
BseBI CCWGG 1 cut(s) 735
BseDI CCNNGG 2 cut(s) 298, 555
BseGI GGATG 2 cut(s) 601, 632
BseLI CCNNNNNNNGG 3 cut(s) 63, 556, 627
BseMI GCAATG 2 cut(s) 225, 711
BseMII CTCAG 1 cut(s) 488
BseNI ACTGG 2 cut(s) 55, 835
BseRI GAGGAG 2 cut(s) 572, 596
BseXI GCAGC 3 cut(s) 164, 677, 778
BsgI GTGCAG 1 cut(s) 777
BshFI GGCC 2 cut(s) 334, 631
BsiSI CCGG 2 cut(s) 768, 856
BslFI GGGAC 1 cut(s) 504
BslI CCNNNNNNNGG 3 cut(s) 63, 556, 627
BsmAI GTCTC 3 cut(s) 365, 481, 493
BsmFI GGGAC 1 cut(s) 504
BsnI GGCC 2 cut(s) 334, 631
Bsp143I GATC 3 cut(s) 302, 751, 799
BspACI CCGC 2 cut(s) 5, 377
BspANI GGCC 2 cut(s) 334, 631
BspCNI CTCAG 1 cut(s) 489
BspLI GGNNCC 1 cut(s) 55
BspPI GGATC 2 cut(s) 310, 794
BsrDI GCAATG 2 cut(s) 225, 711
BsrI ACTGG 2 cut(s) 55, 835
BssECI CCNNGG 2 cut(s) 298, 555
BssMI GATC 3 cut(s) 302, 751, 799
BssNAI GTATAC 1 cut(s) 310
BssT1I CCWWGG 1 cut(s) 298
Bst1107I GTATAC 1 cut(s) 310
Bst2UI CCWGG 1 cut(s) 735
Bst4CI ACNGT 1 cut(s) 781
Bst6I CTCTTC 3 cut(s) 131, 484, 858
BstAPI GCANNNNNTGC 1 cut(s) 131
BstC8I GCNNGC 1 cut(s) 813
BstDEI CTNAG 1 cut(s) 497
BstENI CCTNNNNNAGG 1 cut(s) 554
BstF5I GGATG 2 cut(s) 601, 632
BstKTI GATC 3 cut(s) 305, 754, 802
BstMAI GTCTC 3 cut(s) 365, 481, 493
BstMBI GATC 3 cut(s) 302, 751, 799
BstMWI GCNNNNNNNGC 5 cut(s) 131, 236, 674, 722, 821
BstNI CCWGG 1 cut(s) 735
BstSCI CCNGG 2 cut(s) 733, 767
BstV1I GCAGC 3 cut(s) 164, 677, 778
BstV2I GAAGAC 1 cut(s) 184
BstX2I RGATCY 3 cut(s) 302, 751, 799
BstYI RGATCY 3 cut(s) 302, 751, 799
BstZ17I GTATAC 1 cut(s) 310
BsuI GTATCC 1 cut(s) 714
BsuRI GGCC 2 cut(s) 334, 631
BtsCI GGATG 2 cut(s) 601, 632
Cac8I GCNNGC 1 cut(s) 813
Cfr13I GGNCC 2 cut(s) 332, 731
CviAII CATG 2 cut(s) 605, 691
DdeI CTNAG 1 cut(s) 497
DpnI GATC 3 cut(s) 304, 753, 801
DpnII GATC 3 cut(s) 302, 751, 799
DrdI GACNNNNNNGTC 1 cut(s) 575
DseDI GACNNNNNNGTC 1 cut(s) 575
Eam1104I CTCTTC 3 cut(s) 131, 484, 858
EarI CTCTTC 3 cut(s) 131, 484, 858
EciI GGCGGA 1 cut(s) 20
Eco130I CCWWGG 1 cut(s) 298
Eco47I GGWCC 1 cut(s) 731
Eco57I CTGAAG 1 cut(s) 474
EcoNI CCTNNNNNAGG 1 cut(s) 554
EcoRII CCWGG 1 cut(s) 733
EcoT14I CCWWGG 1 cut(s) 298
ErhI CCWWGG 1 cut(s) 298
FaeI CATG 2 cut(s) 608, 694
FalI AAGNNNNNCTT 2 cut(s) 654, 686
FaqI GGGAC 1 cut(s) 504
FatI CATG 2 cut(s) 604, 690
FblI GTMKAC 1 cut(s) 309
Fnu4HI GCNGC 3 cut(s) 153, 666, 792
FokI GGATG 2 cut(s) 608, 639
Fsp4HI GCNGC 3 cut(s) 153, 666, 792
FspBI CTAG 6 cut(s) 203, 222, 533, 575, 590, 797
GluI GCNGC 3 cut(s) 153, 666, 792
GsuI CTGGAG 1 cut(s) 72
HaeIII GGCC 2 cut(s) 334, 631
HapII CCGG 2 cut(s) 768, 856
Hin1II CATG 2 cut(s) 608, 694
HincII GTYRAC 2 cut(s) 283, 870
HindII GTYRAC 2 cut(s) 283, 870
HindIII AAGCTT 1 cut(s) 266
HinfI GANTC 6 cut(s) 294, 408, 608, 687, 772, 866
HpaI GTTAAC 1 cut(s) 283
HpaII CCGG 2 cut(s) 768, 856
HphI GGTGA 1 cut(s) 544
Hpy166II GTNNAC 4 cut(s) 283, 310, 568, 870
Hpy188I TCNGA 3 cut(s) 184, 263, 454
Hpy188III TCNNGA 1 cut(s) 590
Hpy8I GTNNAC 4 cut(s) 283, 310, 568, 870
HpyAV CCTTC 1 cut(s) 520
HpyCH4III ACNGT 1 cut(s) 781
HpyCH4V TGCA 4 cut(s) 41, 290, 361, 794
HpyF10VI GCNNNNNNNGC 5 cut(s) 131, 236, 674, 722, 821
HpyF3I CTNAG 1 cut(s) 497
Hsp92II CATG 2 cut(s) 608, 694
KspAI GTTAAC 1 cut(s) 283
Kzo9I GATC 3 cut(s) 302, 751, 799
LmnI GCTCC 3 cut(s) 53, 463, 529
Lsp1109I GCAGC 3 cut(s) 164, 677, 778
LweI GCATC 1 cut(s) 121
MaeI CTAG 6 cut(s) 203, 222, 533, 575, 590, 797
MaeIII GTNAC 3 cut(s) 46, 208, 365
MalI GATC 3 cut(s) 304, 753, 801
MboI GATC 3 cut(s) 302, 751, 799
MboII GAAGA 6 cut(s) 46, 148, 184, 471, 797, 875
MflI RGATCY 3 cut(s) 302, 751, 799
MluCI AATT 4 cut(s) 19, 89, 257, 834
MlyI GAGTC 3 cut(s) 303, 696, 875
MnlI CCTC 7 cut(s) 134, 323, 493, 550, 560, 574, 586
MseI TTAA 5 cut(s) 119, 282, 345, 672, 852
MspA1I CMGCKG 3 cut(s) 155, 377, 496
MspI CCGG 2 cut(s) 768, 856
MspR9I CCNGG 2 cut(s) 735, 769
MvaI CCWGG 1 cut(s) 735
MwoI GCNNNNNNNGC 5 cut(s) 131, 236, 674, 722, 821
NciI CCSGG 1 cut(s) 769
NdeII GATC 3 cut(s) 302, 751, 799
NlaIII CATG 2 cut(s) 608, 694
NlaIV GGNNCC 1 cut(s) 55
PfeI GAWTC 3 cut(s) 408, 608, 772
PflMI CCANNNNNTGG 1 cut(s) 627
PkrI GCNGC 3 cut(s) 154, 667, 793
PleI GAGTC 3 cut(s) 302, 695, 874
PpsI GAGTC 3 cut(s) 302, 695, 874
Psp6I CCWGG 1 cut(s) 733
PspGI CCWGG 1 cut(s) 733
PspN4I GGNNCC 1 cut(s) 55
PspPI GGNCC 2 cut(s) 332, 731
PsuI RGATCY 3 cut(s) 302, 751, 799
PvuII CAGCTG 2 cut(s) 155, 496
SaqAI TTAA 5 cut(s) 119, 282, 345, 672, 852
SatI GCNGC 3 cut(s) 153, 666, 792
Sau3AI GATC 3 cut(s) 302, 751, 799
Sau96I GGNCC 2 cut(s) 332, 731
SchI GAGTC 3 cut(s) 303, 696, 875
ScrFI CCNGG 2 cut(s) 735, 769
SfaNI GCATC 1 cut(s) 121
SinI GGWCC 1 cut(s) 731
SmlI CTYRAG 1 cut(s) 74
SmoI CTYRAG 1 cut(s) 74
Sse9I AATT 4 cut(s) 19, 89, 257, 834
SsiI CCGC 2 cut(s) 5, 377
SspI AATATT 1 cut(s) 343
SspMI CTAG 6 cut(s) 203, 222, 533, 575, 590, 797
StyD4I CCNGG 2 cut(s) 733, 767
StyI CCWWGG 1 cut(s) 298
TaaI ACNGT 1 cut(s) 781
TaqI TCGA 2 cut(s) 579, 611
TasI AATT 4 cut(s) 19, 89, 257, 834
TfiI GAWTC 3 cut(s) 408, 608, 772
Tru1I TTAA 5 cut(s) 119, 282, 345, 672, 852
Tru9I TTAA 5 cut(s) 119, 282, 345, 672, 852
TseI GCWGC 3 cut(s) 152, 665, 791
TspDTI ATGAA 1 cut(s) 876
Van91I CCANNNNNTGG 1 cut(s) 627
VpaK11BI GGWCC 1 cut(s) 731
XagI CCTNNNNNAGG 1 cut(s) 554
XapI RAATTY 1 cut(s) 834
XbaI TCTAGA 1 cut(s) 589
XmiI GTMKAC 1 cut(s) 309
XspI CTAG 6 cut(s) 203, 222, 533, 575, 590, 797
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.