Rw7G022380

Salutaridine reductase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0001077
Physical Location & Seq
Forward (+)
26317105 .. 26318571
1467 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G022380.1

Sequence Viewer

Length: 906 bp
ATGGCAGAAATAACAACCATCAGTTTTGGGTCCAAGAGGATTGCTGTTGTTACTGGAGCCAACAAAGGGATTGGACTTGAGATTAGTAGGCAATTAGCTTATAATGGAGTTGGGGTGGTACTAACAGCAAGAGATGTGAAGAGAGGCACAGAAGCTGTTGAAAAGCTTAAGGCCTCTGGTTTCTCTGATGTGGTCTTTCATCAGCTAGATGTAAATAACCCAACTACCATTGCTTCTCTGGCAGATTTTCTCAAAACTCAGTTTGGAAAGCTTGACATTTTGGTTAACAATGCAGGAGTCCTTGGATCCACATACCTCACCGATAACGTCGTAGTGGATCGTCAAAATGTTTTAGGTTCTACATCACTGAAAGAAGTTTTAGTGCAAACATACGAAACGGCAGAGGATTGCTTGAAAACAAACTATTATGGAATCAAGCAACTCACAGAAGCACTTCTTCCCCTTCTTCAAAAATCAGAAGCAGCAAGGATAGTAAATGTCTCTTCAGGACTTGGACAGCTAAGGGTTATTGCAAATGAGAGAGCCAAGAAAGAGCTAGGAGATGTCAATAACCTCACCGAGAAAAAAGTGGACAAACTGGTTGAGGAATTTCTGGAGGATGTGAAGCAGGATTTGATAGAATCCAAAGGCTGGCCTCTGAACATATCTTCTTACATTGTATCAAAAGCAGCTTTGAATGCTTATACAAGAGTCTTGGCAAAGAAGTATCCTGAAATTGCTACGAACGCAGTTAGTCCTGGCTTTACCAAAACAGATATCAACCAAAATACTGGGATTAACACAGTTGAAGAAGGTGCGGAAGGTCCTGTGAAGCTGGCTTTGATAGCTGACACTGGAATTTCTGGCCTCTACTTCGAAATGACTGAAGAGTCAACCTTTGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00902 ko01110 map00902 map01110
Pfam Domains
Protein Families
adh_short adh_short_C2

Protein Analysis

301

Amino Acids

32.6

Weight (kDa)

5.15

Isoelectric Point (pI)

26.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
adh_short PF00106 14 - 177 3.4e-28 short chain dehydrogenase
KR PF08659 16 - 117 4.1e-08 KR domain
adh_short_C2 PF13561 19 - 179 2.3e-18 Enoyl-(Acyl carrier protein) reductase
adh_short_C2 PF13561 222 - 264 2.6e-06 Enoyl-(Acyl carrier protein) reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 102
AasI GACNNNNNNGTC 1 cut(s) 889
AccB7I CCANNNNNTGG 1 cut(s) 651
AciI CCGC 1 cut(s) 818
AclWI GGATC 3 cut(s) 300, 313, 345
AcsI RAATTY 2 cut(s) 608, 858
AcuI CTGAAG 2 cut(s) 489, 906
AfaI GTAC 1 cut(s) 120
AfiI CCNNNNNNNGG 2 cut(s) 66, 651
AflII CTTAAG 1 cut(s) 167
AgsI TTSAA 5 cut(s) 161, 415, 470, 697, 809
AjnI CCWGG 1 cut(s) 757
Alw26I GTCTC 1 cut(s) 505
AlwI GGATC 3 cut(s) 300, 313, 345
AlwNI CAGNNNCTG 1 cut(s) 155
AoxI GGCC 3 cut(s) 171, 653, 865
ApeKI GCWGC 2 cut(s) 482, 689
ApoI RAATTY 2 cut(s) 608, 858
Asp700I GAANNNNTTC 1 cut(s) 453
AspS9I GGNCC 2 cut(s) 30, 824
AsuHPI GGTGA 2 cut(s) 310, 568
AsuII TTCGAA 1 cut(s) 876
AvaII GGWCC 2 cut(s) 30, 824
BamHI GGATCC 1 cut(s) 305
BbvI GCAGC 2 cut(s) 494, 701
BccI CCATC 1 cut(s) 26
BceAI ACGGC 1 cut(s) 414
BciT130I CCWGG 1 cut(s) 759
BciVI GTATCC 1 cut(s) 738
BcoDI GTCTC 1 cut(s) 505
BfaI CTAG 2 cut(s) 206, 557
BfrI CTTAAG 1 cut(s) 167
BfuI GTATCC 1 cut(s) 738
BisI GCNGC 2 cut(s) 483, 690
BlsI GCNGC 2 cut(s) 484, 691
Bme1390I CCNGG 1 cut(s) 759
Bme18I GGWCC 2 cut(s) 30, 824
BmgT120I GGNCC 2 cut(s) 30, 824
BmiI GGNNCC 3 cut(s) 31, 58, 307
BmrFI CCNGG 1 cut(s) 759
BmrI ACTGGG 1 cut(s) 801
BmuI ACTGGG 1 cut(s) 801
BpmI CTGGAG 2 cut(s) 75, 635
Bpu10I CCTNAGC 1 cut(s) 521
Bpu14I TTCGAA 1 cut(s) 876
BpuEI CTTGAG 1 cut(s) 98
BsaJI CCNNGG 1 cut(s) 301
BsaXI ACNNNNNCTCC 2 cut(s) 99, 129
Bsc4I CCNNNNNNNGG 2 cut(s) 66, 651
Bse1I ACTGG 4 cut(s) 58, 603, 796, 859
Bse3DI GCAATG 1 cut(s) 228
BseBI CCWGG 1 cut(s) 759
BseDI CCNNGG 1 cut(s) 301
BseGI GGATG 1 cut(s) 625
BseLI CCNNNNNNNGG 2 cut(s) 66, 651
BseMI GCAATG 1 cut(s) 228
BseMII CTCAG 1 cut(s) 272
BseNI ACTGG 4 cut(s) 58, 603, 796, 859
BseXI GCAGC 2 cut(s) 494, 701
BshFI GGCC 3 cut(s) 173, 655, 867
BslI CCNNNNNNNGG 2 cut(s) 66, 651
BsmAI GTCTC 1 cut(s) 505
BsmI GAATGC 1 cut(s) 703
BsnI GGCC 3 cut(s) 173, 655, 867
Bsp119I TTCGAA 1 cut(s) 876
Bsp143I GATC 2 cut(s) 305, 337
BspACI CCGC 1 cut(s) 818
BspANI GGCC 3 cut(s) 173, 655, 867
BspCNI CTCAG 1 cut(s) 271
BspLI GGNNCC 3 cut(s) 31, 58, 307
BspPI GGATC 3 cut(s) 300, 313, 345
BspT104I TTCGAA 1 cut(s) 876
BspTI CTTAAG 1 cut(s) 167
BsrDI GCAATG 1 cut(s) 228
BsrI ACTGG 4 cut(s) 58, 603, 796, 859
BssECI CCNNGG 1 cut(s) 301
BssMI GATC 2 cut(s) 305, 337
BssT1I CCWWGG 1 cut(s) 301
Bst2UI CCWGG 1 cut(s) 759
Bst4CI ACNGT 1 cut(s) 805
Bst6I CTCTTC 3 cut(s) 134, 508, 882
BstAFI CTTAAG 1 cut(s) 167
BstBI TTCGAA 1 cut(s) 876
BstC8I GCNNGC 2 cut(s) 653, 837
BstDEI CTNAG 2 cut(s) 258, 521
BstF5I GGATG 1 cut(s) 625
BstKTI GATC 2 cut(s) 308, 340
BstMAI GTCTC 1 cut(s) 505
BstMBI GATC 2 cut(s) 305, 337
BstMWI GCNNNNNNNGC 4 cut(s) 239, 698, 746, 845
BstNI CCWGG 1 cut(s) 759
BstSCI CCNGG 1 cut(s) 757
BstV1I GCAGC 2 cut(s) 494, 701
BstX2I RGATCY 1 cut(s) 305
BstXI CCANNNNNNTGG 1 cut(s) 791
BstYI RGATCY 1 cut(s) 305
BsuI GTATCC 1 cut(s) 738
BsuRI GGCC 3 cut(s) 173, 655, 867
BtsCI GGATG 1 cut(s) 625
BtsIMutI CAGTG 2 cut(s) 365, 852
Cac8I GCNNGC 2 cut(s) 653, 837
CaiI CAGNNNCTG 1 cut(s) 155
Cfr13I GGNCC 2 cut(s) 30, 824
Csp6I GTAC 1 cut(s) 119
CviQI GTAC 1 cut(s) 119
DdeI CTNAG 2 cut(s) 258, 521
DpnI GATC 2 cut(s) 307, 339
DpnII GATC 2 cut(s) 305, 337
DrdI GACNNNNNNGTC 1 cut(s) 889
DseDI GACNNNNNNGTC 1 cut(s) 889
Eam1104I CTCTTC 3 cut(s) 134, 508, 882
EarI CTCTTC 3 cut(s) 134, 508, 882
Eco130I CCWWGG 1 cut(s) 301
Eco147I AGGCCT 1 cut(s) 173
Eco32I GATATC 1 cut(s) 778
Eco47I GGWCC 2 cut(s) 30, 824
Eco57I CTGAAG 2 cut(s) 489, 906
EcoO109I RGGNCCY 1 cut(s) 824
EcoRII CCWGG 1 cut(s) 757
EcoRV GATATC 1 cut(s) 778
EcoT14I CCWWGG 1 cut(s) 301
ErhI CCWWGG 1 cut(s) 301
FaiI YATR 6 cut(s) 102, 313, 391, 429, 665, 705
FalI AAGNNNNNCTT 2 cut(s) 441, 473
Fnu4HI GCNGC 2 cut(s) 483, 690
FokI GGATG 1 cut(s) 632
Fsp4HI GCNGC 2 cut(s) 483, 690
FspBI CTAG 2 cut(s) 206, 557
GluI GCNGC 2 cut(s) 483, 690
GsuI CTGGAG 2 cut(s) 75, 635
HaeIII GGCC 3 cut(s) 173, 655, 867
HincII GTYRAC 2 cut(s) 286, 894
HindII GTYRAC 2 cut(s) 286, 894
HindIII AAGCTT 2 cut(s) 164, 269
HinfI GANTC 5 cut(s) 297, 432, 641, 711, 890
HpaI GTTAAC 1 cut(s) 286
HphI GGTGA 2 cut(s) 310, 568
Hpy166II GTNNAC 3 cut(s) 286, 592, 894
Hpy188I TCNGA 3 cut(s) 187, 478, 660
Hpy188III TCNNGA 3 cut(s) 507, 614, 731
Hpy8I GTNNAC 3 cut(s) 286, 592, 894
Hpy99I CGWCG 1 cut(s) 332
HpyAV CCTTC 3 cut(s) 473, 806, 815
HpyCH4III ACNGT 1 cut(s) 805
HpyCH4IV ACGT 1 cut(s) 327
HpyCH4V TGCA 3 cut(s) 293, 385, 533
HpyF10VI GCNNNNNNNGC 4 cut(s) 239, 698, 746, 845
HpyF3I CTNAG 2 cut(s) 258, 521
HpySE526I ACGT 1 cut(s) 327
KspAI GTTAAC 1 cut(s) 286
Kzo9I GATC 2 cut(s) 305, 337
LmnI GCTCC 1 cut(s) 56
Lsp1109I GCAGC 2 cut(s) 494, 701
MaeI CTAG 2 cut(s) 206, 557
MaeII ACGT 1 cut(s) 327
MaeIII GTNAC 1 cut(s) 49
MalI GATC 2 cut(s) 307, 339
MboI GATC 2 cut(s) 305, 337
MboII GAAGA 7 cut(s) 151, 449, 458, 495, 660, 821, 899
MflI RGATCY 1 cut(s) 305
MluCI AATT 4 cut(s) 92, 608, 735, 858
MlyI GAGTC 3 cut(s) 306, 720, 899
MroXI GAANNNNTTC 1 cut(s) 453
MseI TTAA 3 cut(s) 168, 285, 798
MspCI CTTAAG 1 cut(s) 167
MspR9I CCNGG 1 cut(s) 759
Mva1269I GAATGC 1 cut(s) 703
MvaI CCWGG 1 cut(s) 759
MwoI GCNNNNNNNGC 4 cut(s) 239, 698, 746, 845
NdeII GATC 2 cut(s) 305, 337
NlaIV GGNNCC 3 cut(s) 31, 58, 307
NspV TTCGAA 1 cut(s) 876
PceI AGGCCT 1 cut(s) 173
PctI GAATGC 1 cut(s) 703
PdmI GAANNNNTTC 1 cut(s) 453
PfeI GAWTC 2 cut(s) 432, 641
PflMI CCANNNNNTGG 1 cut(s) 651
PkrI GCNGC 2 cut(s) 484, 691
PleI GAGTC 3 cut(s) 305, 719, 898
PpsI GAGTC 3 cut(s) 305, 719, 898
PpuMI RGGWCCY 1 cut(s) 824
PsiI TTATAA 1 cut(s) 102
Psp5II RGGWCCY 1 cut(s) 824
Psp6I CCWGG 1 cut(s) 757
PspGI CCWGG 1 cut(s) 757
PspN4I GGNNCC 3 cut(s) 31, 58, 307
PspPI GGNCC 2 cut(s) 30, 824
PspPPI RGGWCCY 1 cut(s) 824
PstNI CAGNNNCTG 1 cut(s) 155
PsuI RGATCY 1 cut(s) 305
RsaI GTAC 1 cut(s) 120
RsaNI GTAC 1 cut(s) 119
SaqAI TTAA 3 cut(s) 168, 285, 798
SatI GCNGC 2 cut(s) 483, 690
Sau3AI GATC 2 cut(s) 305, 337
Sau96I GGNCC 2 cut(s) 30, 824
SchI GAGTC 3 cut(s) 306, 720, 899
ScrFI CCNGG 1 cut(s) 759
SfuI TTCGAA 1 cut(s) 876
SinI GGWCC 2 cut(s) 30, 824
SmlI CTYRAG 2 cut(s) 77, 167
SmoI CTYRAG 2 cut(s) 77, 167
Sse9I AATT 4 cut(s) 92, 608, 735, 858
SseBI AGGCCT 1 cut(s) 173
SsiI CCGC 1 cut(s) 818
SspMI CTAG 2 cut(s) 206, 557
StuI AGGCCT 1 cut(s) 173
StyD4I CCNGG 1 cut(s) 757
StyI CCWWGG 1 cut(s) 301
TaaI ACNGT 1 cut(s) 805
TaiI ACGT 1 cut(s) 330
TaqI TCGA 1 cut(s) 876
TasI AATT 4 cut(s) 92, 608, 735, 858
TfiI GAWTC 2 cut(s) 432, 641
Tru1I TTAA 3 cut(s) 168, 285, 798
Tru9I TTAA 3 cut(s) 168, 285, 798
TscAI CASTG 2 cut(s) 372, 859
TseI GCWGC 2 cut(s) 482, 689
TspDTI ATGAA 1 cut(s) 188
TspRI CASTG 2 cut(s) 372, 859
Van91I CCANNNNNTGG 1 cut(s) 651
Vha464I CTTAAG 1 cut(s) 167
VpaK11BI GGWCC 2 cut(s) 30, 824
XapI RAATTY 2 cut(s) 608, 858
XcmI CCANNNNNNNNNTGG 1 cut(s) 235
XmnI GAANNNNTTC 1 cut(s) 453
XspI CTAG 2 cut(s) 206, 557
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.