Rh7BG256000

Salutaridine reductase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0001077
Physical Location & Seq
Forward (+)
23306803 .. 23308252
1450 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG256000.1

Sequence Viewer

Length: 831 bp
ATGGCAGAAACAACCATCAGTTTTGGGTCCAAGAGGATTGCTGTTGTTACTGGAGCCAACAAAGGGATTGGACTTGAGATTAGTAGGCAATTAGCTTCTGATGGAGTTGGGGTGGTACTAACAGCAAGAGATGTGAAGAGAGGCACAGAAGCTGTTCAAAAGCTTAAGGCCTCTGGTTTCTCTGATGTGGTCTTTCATCAGCTAGATGTTAACAATGCAGGAGTCCTTGGATCCACATACCTCACCGATAACGTCGTAGTGGATCGTCAAAATGTTTTAGGTTCTACATCACTGAAAGAAGTTTTAGTGCAAACATACGAAACGGCAGAGGATTGCTTGAAAACAAACTATTATGGAATCAAGCAACTCACAGAAGCACTTCTTCCCCTTCTTCAAAAATCAGAAGCAGCTAGGATAGTAAATGTCTCTTCAGCACTTGGACAGCTAAGGGTTATTGCAAATGAGAAAGCCAAGAAAGAGCTAGGAGATGTCAATAACCTCACCGAGGAAAAAGTGGACAAGCTGGTTGAGGAATTTCTGGAGGATGTGAAGCAGGATTTGATAGAATCCAAAGGCTGGCCTCTAAACATATCTTCTTACATTGTATCAAAAGCAGCTCTGAATGCTTATACAAGAGTCTTGGCAAAGAAGTATCCTGAAATTGCTACAAACGCAGTTAGTCCTGGCTTTACCAAAACAGATATCAACCAAAATACTGGGATTAACACTGTTGAAGAAGGTGCAGAAGGTCCTGTGAAGCTGGCTTTGATAGCTGACACTGAAATTTCTGGCCTCTACTTTGAAATGACTGAAGAGTCAACCTTTGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00902 ko01110 map00902 map01110
Pfam Domains
Protein Families
adh_short adh_short_C2

Protein Analysis

276

Amino Acids

29.87

Weight (kDa)

4.92

Isoelectric Point (pI)

32.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
adh_short PF00106 13 - 152 5.7e-18 short chain dehydrogenase
adh_short_C2 PF13561 18 - 154 2.1e-09 Enoyl-(Acyl carrier protein) reductase
adh_short_C2 PF13561 196 - 239 2e-06 Enoyl-(Acyl carrier protein) reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 814
AccB7I CCANNNNNTGG 1 cut(s) 576
AclWI GGATC 3 cut(s) 225, 238, 270
AcsI RAATTY 2 cut(s) 533, 783
AcuI CTGAAG 2 cut(s) 414, 831
AfaI GTAC 1 cut(s) 117
AfiI CCNNNNNNNGG 3 cut(s) 63, 505, 576
AflII CTTAAG 1 cut(s) 164
AgsI TTSAA 5 cut(s) 158, 340, 395, 734, 803
AjnI CCWGG 1 cut(s) 682
Alw26I GTCTC 1 cut(s) 430
AlwI GGATC 3 cut(s) 225, 238, 270
AlwNI CAGNNNCTG 1 cut(s) 152
AoxI GGCC 3 cut(s) 168, 578, 790
ApeKI GCWGC 2 cut(s) 407, 614
ApoI RAATTY 2 cut(s) 533, 783
Asp700I GAANNNNTTC 2 cut(s) 153, 378
AspS9I GGNCC 2 cut(s) 27, 749
AsuHPI GGTGA 2 cut(s) 235, 493
AvaII GGWCC 2 cut(s) 27, 749
BamHI GGATCC 1 cut(s) 230
BbvI GCAGC 2 cut(s) 419, 626
BccI CCATC 2 cut(s) 23, 95
BceAI ACGGC 1 cut(s) 339
BciT130I CCWGG 1 cut(s) 684
BciVI GTATCC 1 cut(s) 663
BcoDI GTCTC 1 cut(s) 430
BfaI CTAG 3 cut(s) 203, 411, 482
BfrI CTTAAG 1 cut(s) 164
BfuI GTATCC 1 cut(s) 663
BisI GCNGC 2 cut(s) 408, 615
BlsI GCNGC 2 cut(s) 409, 616
Bme1390I CCNGG 1 cut(s) 684
Bme18I GGWCC 2 cut(s) 27, 749
BmgT120I GGNCC 2 cut(s) 27, 749
BmiI GGNNCC 3 cut(s) 28, 55, 232
BmrFI CCNGG 1 cut(s) 684
BmrI ACTGGG 1 cut(s) 726
BmuI ACTGGG 1 cut(s) 726
BpmI CTGGAG 2 cut(s) 72, 560
Bpu10I CCTNAGC 1 cut(s) 446
BpuEI CTTGAG 1 cut(s) 95
BsaJI CCNNGG 2 cut(s) 226, 504
BsaXI ACNNNNNCTCC 2 cut(s) 96, 126
Bsc4I CCNNNNNNNGG 3 cut(s) 63, 505, 576
Bse1I ACTGG 2 cut(s) 55, 721
BseBI CCWGG 1 cut(s) 684
BseDI CCNNGG 2 cut(s) 226, 504
BseGI GGATG 1 cut(s) 550
BseLI CCNNNNNNNGG 3 cut(s) 63, 505, 576
BseNI ACTGG 2 cut(s) 55, 721
BseXI GCAGC 2 cut(s) 419, 626
BsgI GTGCAG 1 cut(s) 762
BshFI GGCC 3 cut(s) 170, 580, 792
BslI CCNNNNNNNGG 3 cut(s) 63, 505, 576
BsmAI GTCTC 1 cut(s) 430
BsmI GAATGC 1 cut(s) 628
BsnI GGCC 3 cut(s) 170, 580, 792
Bsp143I GATC 2 cut(s) 230, 262
BspANI GGCC 3 cut(s) 170, 580, 792
BspLI GGNNCC 3 cut(s) 28, 55, 232
BspPI GGATC 3 cut(s) 225, 238, 270
BspTI CTTAAG 1 cut(s) 164
BsrI ACTGG 2 cut(s) 55, 721
BssECI CCNNGG 2 cut(s) 226, 504
BssMI GATC 2 cut(s) 230, 262
BssT1I CCWWGG 1 cut(s) 226
Bst2UI CCWGG 1 cut(s) 684
Bst4CI ACNGT 1 cut(s) 730
Bst6I CTCTTC 3 cut(s) 131, 433, 807
BstAFI CTTAAG 1 cut(s) 164
BstC8I GCNNGC 2 cut(s) 578, 762
BstDEI CTNAG 1 cut(s) 446
BstENI CCTNNNNNAGG 1 cut(s) 503
BstF5I GGATG 1 cut(s) 550
BstKTI GATC 2 cut(s) 233, 265
BstMAI GTCTC 1 cut(s) 430
BstMBI GATC 2 cut(s) 230, 262
BstMWI GCNNNNNNNGC 3 cut(s) 623, 671, 770
BstNI CCWGG 1 cut(s) 684
BstSCI CCNGG 1 cut(s) 682
BstV1I GCAGC 2 cut(s) 419, 626
BstX2I RGATCY 1 cut(s) 230
BstXI CCANNNNNNTGG 1 cut(s) 716
BstYI RGATCY 1 cut(s) 230
BsuI GTATCC 1 cut(s) 663
BsuRI GGCC 3 cut(s) 170, 580, 792
BtsCI GGATG 1 cut(s) 550
BtsIMutI CAGTG 3 cut(s) 290, 726, 777
Cac8I GCNNGC 2 cut(s) 578, 762
CaiI CAGNNNCTG 1 cut(s) 152
Cfr13I GGNCC 2 cut(s) 27, 749
Csp6I GTAC 1 cut(s) 116
CviQI GTAC 1 cut(s) 116
DdeI CTNAG 1 cut(s) 446
DpnI GATC 2 cut(s) 232, 264
DpnII GATC 2 cut(s) 230, 262
DrdI GACNNNNNNGTC 1 cut(s) 814
DseDI GACNNNNNNGTC 1 cut(s) 814
Eam1104I CTCTTC 3 cut(s) 131, 433, 807
EarI CTCTTC 3 cut(s) 131, 433, 807
Eco130I CCWWGG 1 cut(s) 226
Eco147I AGGCCT 1 cut(s) 170
Eco32I GATATC 1 cut(s) 703
Eco47I GGWCC 2 cut(s) 27, 749
Eco57I CTGAAG 2 cut(s) 414, 831
EcoNI CCTNNNNNAGG 1 cut(s) 503
EcoO109I RGGNCCY 1 cut(s) 749
EcoRII CCWGG 1 cut(s) 682
EcoRV GATATC 1 cut(s) 703
EcoT14I CCWWGG 1 cut(s) 226
ErhI CCWWGG 1 cut(s) 226
FaiI YATR 5 cut(s) 238, 316, 354, 590, 630
FalI AAGNNNNNCTT 2 cut(s) 366, 398
Fnu4HI GCNGC 2 cut(s) 408, 615
FokI GGATG 1 cut(s) 557
Fsp4HI GCNGC 2 cut(s) 408, 615
FspBI CTAG 3 cut(s) 203, 411, 482
GluI GCNGC 2 cut(s) 408, 615
GsuI CTGGAG 2 cut(s) 72, 560
HaeIII GGCC 3 cut(s) 170, 580, 792
HincII GTYRAC 2 cut(s) 211, 819
HindII GTYRAC 2 cut(s) 211, 819
HindIII AAGCTT 1 cut(s) 161
HinfI GANTC 5 cut(s) 222, 357, 566, 636, 815
HpaI GTTAAC 1 cut(s) 211
HphI GGTGA 2 cut(s) 235, 493
Hpy166II GTNNAC 3 cut(s) 211, 517, 819
Hpy188I TCNGA 4 cut(s) 100, 184, 403, 621
Hpy188III TCNNGA 2 cut(s) 539, 656
Hpy8I GTNNAC 3 cut(s) 211, 517, 819
Hpy99I CGWCG 1 cut(s) 257
HpyAV CCTTC 3 cut(s) 398, 731, 740
HpyCH4III ACNGT 1 cut(s) 730
HpyCH4IV ACGT 1 cut(s) 252
HpyCH4V TGCA 4 cut(s) 218, 310, 458, 743
HpyF10VI GCNNNNNNNGC 3 cut(s) 623, 671, 770
HpyF3I CTNAG 1 cut(s) 446
HpySE526I ACGT 1 cut(s) 252
KspAI GTTAAC 1 cut(s) 211
Kzo9I GATC 2 cut(s) 230, 262
LmnI GCTCC 1 cut(s) 53
Lsp1109I GCAGC 2 cut(s) 419, 626
MaeI CTAG 3 cut(s) 203, 411, 482
MaeII ACGT 1 cut(s) 252
MaeIII GTNAC 1 cut(s) 46
MalI GATC 2 cut(s) 232, 264
MboI GATC 2 cut(s) 230, 262
MboII GAAGA 7 cut(s) 148, 374, 383, 420, 585, 746, 824
MflI RGATCY 1 cut(s) 230
MluCI AATT 4 cut(s) 89, 533, 660, 783
MlyI GAGTC 3 cut(s) 231, 645, 824
MroXI GAANNNNTTC 2 cut(s) 153, 378
MseI TTAA 3 cut(s) 165, 210, 723
MspCI CTTAAG 1 cut(s) 164
MspR9I CCNGG 1 cut(s) 684
Mva1269I GAATGC 1 cut(s) 628
MvaI CCWGG 1 cut(s) 684
MwoI GCNNNNNNNGC 3 cut(s) 623, 671, 770
NdeII GATC 2 cut(s) 230, 262
NlaIV GGNNCC 3 cut(s) 28, 55, 232
PceI AGGCCT 1 cut(s) 170
PctI GAATGC 1 cut(s) 628
PdmI GAANNNNTTC 2 cut(s) 153, 378
PfeI GAWTC 2 cut(s) 357, 566
PflMI CCANNNNNTGG 1 cut(s) 576
PkrI GCNGC 2 cut(s) 409, 616
PleI GAGTC 3 cut(s) 230, 644, 823
PpsI GAGTC 3 cut(s) 230, 644, 823
PpuMI RGGWCCY 1 cut(s) 749
Psp5II RGGWCCY 1 cut(s) 749
Psp6I CCWGG 1 cut(s) 682
PspGI CCWGG 1 cut(s) 682
PspN4I GGNNCC 3 cut(s) 28, 55, 232
PspPI GGNCC 2 cut(s) 27, 749
PspPPI RGGWCCY 1 cut(s) 749
PstNI CAGNNNCTG 1 cut(s) 152
PsuI RGATCY 1 cut(s) 230
RsaI GTAC 1 cut(s) 117
RsaNI GTAC 1 cut(s) 116
SaqAI TTAA 3 cut(s) 165, 210, 723
SatI GCNGC 2 cut(s) 408, 615
Sau3AI GATC 2 cut(s) 230, 262
Sau96I GGNCC 2 cut(s) 27, 749
SchI GAGTC 3 cut(s) 231, 645, 824
ScrFI CCNGG 1 cut(s) 684
SinI GGWCC 2 cut(s) 27, 749
SmlI CTYRAG 2 cut(s) 74, 164
SmoI CTYRAG 2 cut(s) 74, 164
Sse9I AATT 4 cut(s) 89, 533, 660, 783
SseBI AGGCCT 1 cut(s) 170
SspMI CTAG 3 cut(s) 203, 411, 482
StuI AGGCCT 1 cut(s) 170
StyD4I CCNGG 1 cut(s) 682
StyI CCWWGG 1 cut(s) 226
TaaI ACNGT 1 cut(s) 730
TaiI ACGT 1 cut(s) 255
TasI AATT 4 cut(s) 89, 533, 660, 783
TfiI GAWTC 2 cut(s) 357, 566
Tru1I TTAA 3 cut(s) 165, 210, 723
Tru9I TTAA 3 cut(s) 165, 210, 723
TscAI CASTG 3 cut(s) 297, 733, 784
TseI GCWGC 2 cut(s) 407, 614
TspDTI ATGAA 1 cut(s) 185
TspRI CASTG 3 cut(s) 297, 733, 784
Van91I CCANNNNNTGG 1 cut(s) 576
Vha464I CTTAAG 1 cut(s) 164
VpaK11BI GGWCC 2 cut(s) 27, 749
XagI CCTNNNNNAGG 1 cut(s) 503
XapI RAATTY 2 cut(s) 533, 783
XmnI GAANNNNTTC 2 cut(s) 153, 378
XspI CTAG 3 cut(s) 203, 411, 482
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.