Rorug07G0048300

Salutaridine reductase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0001077
Physical Location & Seq
Reverse (-)
3498463 .. 3501702
3240 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0048300.1

Sequence Viewer

Length: 909 bp
ATGTTTGGTTTAAAGAAGTCCCCGTTAAAGGCTGCTAAGCGTAACTCAGTTGACCCTCTATATCCTGCTTCCTCTGGCTCAAACCCGTTTGACGATGAGCCTGCACAAGTTAGACAGAATGAGAAGCATGCACTAGTTGGACAGAATGATAAGCAGGCACTATTTGGACAGGATACTAGCACCAATCCTTTTGATGATGATCAAGGAAAAGAGAATACTTCTTTGTATTCATACAAGAGTACTTACTCCGAGAGAAACAAGTATAAGAATGACTTCCGCAATTCAGGAGGCGTTGAAAATCAATCGGTGCAAGAGTTGGAAAGTTATGCAGTGTATAAGGCTGAGGAGACTACAAACTCAGTCCACAGCTGCCTGAGGATTGCTGAAGATATACGTGAGGATGCTACCAAGACTCTGGTCATGTTGCATCACCAGGGTGACCAAATCACCAGGACCCACTTTGTTGCTGCTGACATTGAACAGGATCTCAGTCGGGGTGAAAAGCTTCTGGGGAGTCTCGGGGGCATCTTCTCTAAGACTTGGAAGCCAAAGAAGGGTCGCACAATAACTGGCCCTGTCATCACACCAGATGCTGCAATCAAAAAGAGCAGCCAGTTGGAGAGGGAAAAGCTGGGACTGACTCCACCCAAGGGATTGTCACATTCACGAACACCACTTCCTGAACCCACCAATGCCCTTCAGAAAGTTGAGGTAGAGAAGGCGAAACAAGATGAAGGACTGTCAGATTTAAGTAATCTGTTGGGAGAGCTGAAGGATATGGCCATTGACATGGGGTCTGAAATCGATAGGCATAACAAAGCTATAGGTCATATGGATGCTGATGTTGATGAGCTCCACTACCGTGTTAGAGGTGCTACTCAACGTGGTCGTCGCTTGCTTGGAAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00902 ko01110 map00902 map01110
Pfam Domains
Protein Families
adh_short adh_short_C2

Protein Analysis

302

Amino Acids

33.55

Weight (kDa)

6.77

Isoelectric Point (pI)

36.19

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 277
AclWI GGATC 1 cut(s) 492
AcoI YGGCCR 1 cut(s) 780
AcuI CTGAAG 3 cut(s) 405, 683, 791
AfaI GTAC 1 cut(s) 241
AfiI CCNNNNNNNGG 3 cut(s) 28, 554, 650
AgsI TTSAA 2 cut(s) 296, 479
AhdI GACNNNNNGTC 1 cut(s) 793
AhlI ACTAGT 1 cut(s) 133
AjnI CCWGG 2 cut(s) 432, 449
AleI CACNNNNGTG 2 cut(s) 435, 861
AloI GAACNNNNNNTCC 2 cut(s) 661, 693
AluBI AGCT 6 cut(s) 369, 505, 631, 769, 821, 853
AluI AGCT 6 cut(s) 369, 505, 631, 769, 821, 853
Alw21I GWGCWC 1 cut(s) 855
Alw26I GTCTC 2 cut(s) 341, 521
AlwI GGATC 1 cut(s) 492
AlwNI CAGNNNCTG 1 cut(s) 593
Ama87I CYCGRG 1 cut(s) 518
AoxI GGCC 2 cut(s) 571, 780
ApeKI GCWGC 5 cut(s) 32, 369, 467, 593, 609
Asp700I GAANNNNTTC 2 cut(s) 272, 504
AspS9I GGNCC 2 cut(s) 453, 572
AsuHPI GGTGA 4 cut(s) 422, 439, 449, 509
AvaI CYCGRG 1 cut(s) 518
AvaII GGWCC 1 cut(s) 453
AxyI CCTNAGG 1 cut(s) 374
BalI TGGCCA 1 cut(s) 782
BanII GRGCYC 1 cut(s) 855
Bbv12I GWGCWC 1 cut(s) 855
BbvCI CCTCAGC 1 cut(s) 342
BbvI GCAGC 5 cut(s) 19, 356, 454, 580, 621
BcgI CGANNNNNNTGC 2 cut(s) 83, 117
BciT130I CCWGG 2 cut(s) 434, 451
BciVI GTATCC 1 cut(s) 166
BclI TGATCA 1 cut(s) 199
BcoDI GTCTC 2 cut(s) 341, 521
BcuI ACTAGT 1 cut(s) 133
BfaI CTAG 2 cut(s) 134, 177
BfmI CTRYAG 1 cut(s) 822
BfuI GTATCC 1 cut(s) 166
BisI GCNGC 5 cut(s) 33, 370, 468, 594, 610
BlpI GCTNAGC 1 cut(s) 36
BlsI GCNGC 5 cut(s) 34, 371, 469, 595, 611
BmcAI AGTACT 1 cut(s) 241
Bme1390I CCNGG 2 cut(s) 434, 451
Bme18I GGWCC 1 cut(s) 453
BmeRI GACNNNNNGTC 1 cut(s) 793
BmeT110I CYCGRG 1 cut(s) 518
BmgT120I GGNCC 2 cut(s) 453, 572
BmiI GGNNCC 1 cut(s) 455
BmrFI CCNGG 2 cut(s) 434, 451
BmsI GCATC 5 cut(s) 391, 436, 534, 580, 826
BoxI GACNNNNGTC 1 cut(s) 416
Bpu10I CCTNAGC 1 cut(s) 342
Bpu1102I GCTNAGC 1 cut(s) 36
Bsa29I ATCGAT 1 cut(s) 804
BsaAI YACGTR 1 cut(s) 395
BsaBI GATNNNNATC 1 cut(s) 198
BsaJI CCNNGG 2 cut(s) 433, 648
Bsc4I CCNNNNNNNGG 3 cut(s) 28, 554, 650
Bse1I ACTGG 2 cut(s) 574, 613
Bse21I CCTNAGG 1 cut(s) 374
Bse8I GATNNNNATC 1 cut(s) 198
BseBI CCWGG 2 cut(s) 434, 451
BseCI ATCGAT 1 cut(s) 804
BseDI CCNNGG 2 cut(s) 433, 648
BseGI GGATG 2 cut(s) 406, 841
BseJI GATNNNNATC 1 cut(s) 198
BseLI CCNNNNNNNGG 3 cut(s) 28, 554, 650
BseMII CTCAG 5 cut(s) 60, 333, 365, 372, 502
BseNI ACTGG 2 cut(s) 574, 613
BseRI GAGGAG 1 cut(s) 359
BseXI GCAGC 5 cut(s) 19, 356, 454, 580, 621
BseYI CCCAGC 1 cut(s) 631
BsgI GTGCAG 1 cut(s) 87
BshFI GGCC 2 cut(s) 573, 782
BshVI ATCGAT 1 cut(s) 804
BsiHKAI GWGCWC 1 cut(s) 855
BsiHKCI CYCGRG 1 cut(s) 518
BslFI GGGAC 2 cut(s) 4, 648
BslI CCNNNNNNNGG 3 cut(s) 28, 554, 650
BsmAI GTCTC 2 cut(s) 341, 521
BsmFI GGGAC 2 cut(s) 4, 648
BsnI GGCC 2 cut(s) 573, 782
BsoBI CYCGRG 1 cut(s) 518
Bsp1286I GDGCHC 1 cut(s) 855
Bsp143I GATC 2 cut(s) 199, 484
Bsp1720I GCTNAGC 1 cut(s) 36
BspACI CCGC 1 cut(s) 277
BspANI GGCC 2 cut(s) 573, 782
BspCNI CTCAG 5 cut(s) 59, 334, 366, 371, 501
BspDI ATCGAT 1 cut(s) 804
BspLI GGNNCC 1 cut(s) 455
BspPI GGATC 1 cut(s) 492
BsrI ACTGG 2 cut(s) 574, 613
BssECI CCNNGG 2 cut(s) 433, 648
BssMI GATC 2 cut(s) 199, 484
BssT1I CCWWGG 1 cut(s) 648
Bst2UI CCWGG 2 cut(s) 434, 451
Bst4CI ACNGT 2 cut(s) 741, 863
BstBAI YACGTR 1 cut(s) 395
BstC8I GCNNGC 4 cut(s) 102, 129, 156, 896
BstDEI CTNAG 7 cut(s) 36, 46, 342, 358, 374, 488, 534
BstEII GGTNACC 1 cut(s) 437
BstF5I GGATG 2 cut(s) 406, 841
BstKTI GATC 2 cut(s) 202, 487
BstMAI GTCTC 2 cut(s) 341, 521
BstMBI GATC 2 cut(s) 199, 484
BstNI CCWGG 2 cut(s) 434, 451
BstNSI RCATGY 1 cut(s) 131
BstPAI GACNNNNGTC 1 cut(s) 416
BstPI GGTNACC 1 cut(s) 437
BstSCI CCNGG 2 cut(s) 432, 449
BstSFI CTRYAG 1 cut(s) 822
BstV1I GCAGC 5 cut(s) 19, 356, 454, 580, 621
BstX2I RGATCY 1 cut(s) 484
BstXI CCANNNNNNTGG 2 cut(s) 415, 790
BstYI RGATCY 1 cut(s) 484
Bsu15I ATCGAT 1 cut(s) 804
Bsu36I CCTNAGG 1 cut(s) 374
BsuI GTATCC 1 cut(s) 166
BsuRI GGCC 2 cut(s) 573, 782
BsuTUI ATCGAT 1 cut(s) 804
BtsCI GGATG 2 cut(s) 406, 841
BtsI GCAGTG 1 cut(s) 336
BtsIMutI CAGTG 1 cut(s) 336
Cac8I GCNNGC 4 cut(s) 102, 129, 156, 896
CaiI CAGNNNCTG 1 cut(s) 593
Cfr13I GGNCC 2 cut(s) 453, 572
ClaI ATCGAT 1 cut(s) 804
Csp6I GTAC 1 cut(s) 240
CviAII CATG 3 cut(s) 128, 421, 790
CviQI GTAC 1 cut(s) 240
DdeI CTNAG 7 cut(s) 36, 46, 342, 358, 374, 488, 534
DpnI GATC 2 cut(s) 201, 486
DpnII GATC 2 cut(s) 199, 484
DraI TTTAAA 1 cut(s) 12
DriI GACNNNNNGTC 1 cut(s) 793
EaeI YGGCCR 1 cut(s) 780
Eam1105I GACNNNNNGTC 1 cut(s) 793
Ecl136II GAGCTC 1 cut(s) 853
Eco130I CCWWGG 1 cut(s) 648
Eco24I GRGCYC 1 cut(s) 855
Eco47I GGWCC 1 cut(s) 453
Eco53kI GAGCTC 1 cut(s) 853
Eco57I CTGAAG 3 cut(s) 405, 683, 791
Eco81I CCTNAGG 1 cut(s) 374
Eco88I CYCGRG 1 cut(s) 518
Eco91I GGTNACC 1 cut(s) 437
EcoICRI GAGCTC 1 cut(s) 853
EcoO109I RGGNCCY 1 cut(s) 453
EcoO65I GGTNACC 1 cut(s) 437
EcoRII CCWGG 2 cut(s) 432, 449
EcoT14I CCWWGG 1 cut(s) 648
EcoT38I GRGCYC 1 cut(s) 855
ErhI CCWWGG 1 cut(s) 648
FaeI CATG 3 cut(s) 131, 424, 793
FalI AAGNNNNNCTT 2 cut(s) 257, 289
FaqI GGGAC 2 cut(s) 4, 648
FatI CATG 3 cut(s) 127, 420, 789
FauNDI CATATG 1 cut(s) 831
FbaI TGATCA 1 cut(s) 199
Fnu4HI GCNGC 5 cut(s) 33, 370, 468, 594, 610
FokI GGATG 2 cut(s) 413, 848
FriOI GRGCYC 1 cut(s) 855
Fsp4HI GCNGC 5 cut(s) 33, 370, 468, 594, 610
FspBI CTAG 2 cut(s) 134, 177
GluI GCNGC 5 cut(s) 33, 370, 468, 594, 610
GsaI CCCAGC 1 cut(s) 635
HaeIII GGCC 2 cut(s) 573, 782
Hin1II CATG 3 cut(s) 131, 424, 793
HincII GTYRAC 1 cut(s) 52
HindII GTYRAC 1 cut(s) 52
HindIII AAGCTT 1 cut(s) 503
HinfI GANTC 3 cut(s) 412, 514, 640
HphI GGTGA 4 cut(s) 422, 439, 449, 509
Hpy166II GTNNAC 2 cut(s) 52, 364
Hpy188I TCNGA 4 cut(s) 250, 702, 745, 799
Hpy188III TCNNGA 3 cut(s) 285, 666, 680
Hpy8I GTNNAC 2 cut(s) 52, 364
Hpy99I CGWCG 1 cut(s) 894
HpyAV CCTTC 5 cut(s) 547, 707, 712, 728, 766
HpyCH4III ACNGT 2 cut(s) 741, 863
HpyCH4IV ACGT 2 cut(s) 394, 883
HpyCH4V TGCA 6 cut(s) 104, 131, 310, 329, 427, 596
HpyF3I CTNAG 7 cut(s) 36, 46, 342, 358, 374, 488, 534
HpySE526I ACGT 2 cut(s) 394, 883
Hsp92II CATG 3 cut(s) 131, 424, 793
Ksp22I TGATCA 1 cut(s) 199
Kzo9I GATC 2 cut(s) 199, 484
LmnI GCTCC 1 cut(s) 858
Lsp1109I GCAGC 5 cut(s) 19, 356, 454, 580, 621
LweI GCATC 5 cut(s) 391, 436, 534, 580, 826
MaeI CTAG 2 cut(s) 134, 177
MaeII ACGT 2 cut(s) 394, 883
MaeIII GTNAC 3 cut(s) 41, 437, 657
MalI GATC 2 cut(s) 201, 486
MboI GATC 2 cut(s) 199, 484
MboII GAAGA 2 cut(s) 398, 520
MflI RGATCY 1 cut(s) 484
MhlI GDGCHC 1 cut(s) 855
MlsI TGGCCA 1 cut(s) 782
MluCI AATT 1 cut(s) 280
MluNI TGGCCA 1 cut(s) 782
MlyI GAGTC 3 cut(s) 406, 523, 634
MmeI TCCRAC 3 cut(s) 118, 297, 597
MnlI CCTC 9 cut(s) 66, 82, 281, 337, 369, 391, 615, 703, 863
Mox20I TGGCCA 1 cut(s) 782
MroXI GAANNNNTTC 2 cut(s) 272, 504
MscI TGGCCA 1 cut(s) 782
MseI TTAA 3 cut(s) 11, 26, 749
MslI CAYNNNNRTG 4 cut(s) 435, 788, 834, 861
Msp20I TGGCCA 1 cut(s) 782
MspA1I CMGCKG 1 cut(s) 369
MspR9I CCNGG 2 cut(s) 434, 451
MvaI CCWGG 2 cut(s) 434, 451
NdeI CATATG 1 cut(s) 831
NdeII GATC 2 cut(s) 199, 484
NlaIII CATG 3 cut(s) 131, 424, 793
NlaIV GGNNCC 1 cut(s) 455
NmuCI GTSAC 2 cut(s) 437, 657
NspI RCATGY 1 cut(s) 131
OliI CACNNNNGTG 2 cut(s) 435, 861
PaeI GCATGC 1 cut(s) 131
PdmI GAANNNNTTC 2 cut(s) 272, 504
PkrI GCNGC 5 cut(s) 34, 371, 469, 595, 611
PleI GAGTC 3 cut(s) 406, 522, 634
PpsI GAGTC 3 cut(s) 406, 522, 634
Ppu21I YACGTR 1 cut(s) 395
PpuMI RGGWCCY 1 cut(s) 453
PshAI GACNNNNGTC 1 cut(s) 416
Psp124BI GAGCTC 1 cut(s) 855
Psp5II RGGWCCY 1 cut(s) 453
Psp6I CCWGG 2 cut(s) 432, 449
PspEI GGTNACC 1 cut(s) 437
PspFI CCCAGC 1 cut(s) 631
PspGI CCWGG 2 cut(s) 432, 449
PspN4I GGNNCC 1 cut(s) 455
PspPI GGNCC 2 cut(s) 453, 572
PspPPI RGGWCCY 1 cut(s) 453
PstNI CAGNNNCTG 1 cut(s) 593
PsuI RGATCY 1 cut(s) 484
PvuII CAGCTG 1 cut(s) 369
RsaI GTAC 1 cut(s) 241
RsaNI GTAC 1 cut(s) 240
RseI CAYNNNNRTG 4 cut(s) 435, 788, 834, 861
SacI GAGCTC 1 cut(s) 855
SaqAI TTAA 3 cut(s) 11, 26, 749
SatI GCNGC 5 cut(s) 33, 370, 468, 594, 610
Sau3AI GATC 2 cut(s) 199, 484
Sau96I GGNCC 2 cut(s) 453, 572
ScaI AGTACT 1 cut(s) 241
SchI GAGTC 3 cut(s) 406, 523, 634
ScrFI CCNGG 2 cut(s) 434, 451
SduI GDGCHC 1 cut(s) 855
SfaNI GCATC 5 cut(s) 391, 436, 534, 580, 826
SfcI CTRYAG 1 cut(s) 822
SinI GGWCC 1 cut(s) 453
SmiMI CAYNNNNRTG 4 cut(s) 435, 788, 834, 861
SpeI ACTAGT 1 cut(s) 133
SphI GCATGC 1 cut(s) 131
Sse9I AATT 1 cut(s) 280
SsiI CCGC 1 cut(s) 277
SspMI CTAG 2 cut(s) 134, 177
SstI GAGCTC 1 cut(s) 855
StyD4I CCNGG 2 cut(s) 432, 449
StyI CCWWGG 1 cut(s) 648
TaaI ACNGT 2 cut(s) 741, 863
TaiI ACGT 2 cut(s) 397, 886
TaqI TCGA 1 cut(s) 804
TasI AATT 1 cut(s) 280
TatI WGTACW 1 cut(s) 239
Tru1I TTAA 3 cut(s) 11, 26, 749
Tru9I TTAA 3 cut(s) 11, 26, 749
TscAI CASTG 1 cut(s) 336
TseFI GTSAC 2 cut(s) 437, 657
TseI GCWGC 5 cut(s) 32, 369, 467, 593, 609
Tsp45I GTSAC 2 cut(s) 437, 657
TspDTI ATGAA 2 cut(s) 219, 747
TspRI CASTG 1 cut(s) 336
VpaK11BI GGWCC 1 cut(s) 453
XceI RCATGY 1 cut(s) 131
XmnI GAANNNNTTC 2 cut(s) 272, 504
XspI CTAG 2 cut(s) 134, 177
ZrmI AGTACT 1 cut(s) 241
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.