RchiOBHm_Chr7g0198551

Salutaridine reductase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0001077
Physical Location & Seq
Reverse (-)
16462537 .. 16464317
1781 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ17764

Sequence Viewer

Length: 855 bp
ATGGCGGAAACTACCATCAATTTTGGGTCAAAGAAGATTGCAGTTGTTACTGGAGCCAACAAAGGGATTGGACTTGAGATTAGTAAGCAATTAGCTTCTAAAGGAGTTGGGGTGGTATTAACAGCAAGAGATGCGAAGAGAGGAACAGAAGCAGCTGAAAGCCTAAAGGCTTCTGGTTTCTCCGATGTGGTCTTCCATCAGCTAGATGTAACTGCCCCAACTAGCATTGCTTCTTTGGCAAACTTTCTCAAAACGCAATTCAGAAAGCTTGACATATTGGTTAACAATGCAGGAGTCCTTGGATCTGTATTCCTCGCAGACGACATAGCTGGGCCTGACAATGTTAAACAAAATACAGTGCAAACTTACGAGACAGCGGCGGATTGTTTGAAAACAAACTATTATGGAATCAAGCAACTCACAGAAGCACTTGTTCCACTACTTCAAAAATCAGAAGCTCCAAAGATAGTCAATGTCTCTTCCACACTGGGACAGCTAAGAGAGCTAGGAGATGCGGATAACCTCACCGAGGAGAAAGTGGACAAGCTAGTTGAGGAATTTCTAGAGGATGTGAAGCATGATTCGATAGAATCCAAAGGATGGCCTTTAGATATATCTGCCTACATTGTATCGAAAGCAGCTCTTAATGCTTATACAAGAGTCGTGGCAAAGAAGTATCCCCACATTGCTATTAACGCAGTTAGTCCAGGTTATACCAAAACAGATCTCAACAATAATACTGGGATTCTCACAGTTGAAGAAGCTGCACTTGGTCCTGTGAAGCTAGCTTTGATAGCCGAAACTGGAATTTCAGGCCTCTTCTTTGACCGGACTGAAGACTCAACCTTTGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00902 ko01110 map00902 map01110
Pfam Domains
Protein Families
adh_short adh_short_C2

Protein Analysis

284

Amino Acids

30.38

Weight (kDa)

5.16

Isoelectric Point (pI)

25.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
adh_short PF00106 12 - 166 7e-29 short chain dehydrogenase
KR PF08659 15 - 106 9e-08 KR domain
adh_short_C2 PF13561 18 - 165 6.7e-19 Enoyl-(Acyl carrier protein) reductase
adh_short_C2 PF13561 204 - 246 1.7e-06 Enoyl-(Acyl carrier protein) reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 600
AciI CCGC 4 cut(s) 5, 377, 380, 515
AclWI GGATC 1 cut(s) 310
AcsI RAATTY 2 cut(s) 557, 807
AcuI CTGAAG 1 cut(s) 855
AfiI CCNNNNNNNGG 3 cut(s) 63, 529, 600
AgsI TTSAA 3 cut(s) 391, 446, 758
AjnI CCWGG 1 cut(s) 706
AjuI GAANNNNNNNTTGG 2 cut(s) 753, 785
Alw26I GTCTC 2 cut(s) 365, 481
AlwI GGATC 1 cut(s) 310
AoxI GGCC 3 cut(s) 332, 602, 814
ApeKI GCWGC 3 cut(s) 152, 638, 764
ApoI RAATTY 2 cut(s) 557, 807
ArsI GACNNNNNNTTYG 1 cut(s) 830
AspS9I GGNCC 2 cut(s) 332, 773
AsuHPI GGTGA 1 cut(s) 517
AsuNHI GCTAGC 1 cut(s) 784
AvaII GGWCC 1 cut(s) 773
BbsI GAAGAC 2 cut(s) 184, 843
BbvI GCAGC 3 cut(s) 164, 650, 751
BccI CCATC 3 cut(s) 23, 204, 594
BciT130I CCWGG 1 cut(s) 708
BciVI GTATCC 1 cut(s) 687
BcoDI GTCTC 2 cut(s) 365, 481
BfaI CTAG 6 cut(s) 203, 222, 506, 548, 563, 785
BfuI GTATCC 1 cut(s) 687
BglII AGATCT 1 cut(s) 724
BisI GCNGC 4 cut(s) 153, 378, 639, 765
BlsI GCNGC 4 cut(s) 154, 379, 640, 766
Bme1390I CCNGG 1 cut(s) 708
Bme18I GGWCC 1 cut(s) 773
BmgT120I GGNCC 2 cut(s) 332, 773
BmiI GGNNCC 1 cut(s) 55
BmrFI CCNGG 1 cut(s) 708
BmrI ACTGGG 2 cut(s) 497, 750
BmsI GCATC 2 cut(s) 121, 502
BmtI GCTAGC 1 cut(s) 788
BmuI ACTGGG 2 cut(s) 497, 750
BpiI GAAGAC 2 cut(s) 184, 843
BpmI CTGGAG 1 cut(s) 72
BpuEI CTTGAG 1 cut(s) 95
BsaJI CCNNGG 2 cut(s) 298, 528
BsaWI WCCGGW 1 cut(s) 828
BsaXI ACNNNNNCTCC 2 cut(s) 96, 126
Bsc4I CCNNNNNNNGG 3 cut(s) 63, 529, 600
Bse1I ACTGG 4 cut(s) 55, 492, 745, 808
Bse3DI GCAATG 2 cut(s) 225, 684
BseBI CCWGG 1 cut(s) 708
BseDI CCNNGG 2 cut(s) 298, 528
BseGI GGATG 2 cut(s) 574, 605
BseLI CCNNNNNNNGG 3 cut(s) 63, 529, 600
BseMI GCAATG 2 cut(s) 225, 684
BseNI ACTGG 4 cut(s) 55, 492, 745, 808
BseRI GAGGAG 1 cut(s) 545
BseXI GCAGC 3 cut(s) 164, 650, 751
BseYI CCCAGC 1 cut(s) 329
BsgI GTGCAG 1 cut(s) 750
BshFI GGCC 3 cut(s) 334, 604, 816
BsiSI CCGG 1 cut(s) 829
BslFI GGGAC 1 cut(s) 504
BslI CCNNNNNNNGG 3 cut(s) 63, 529, 600
BsmAI GTCTC 2 cut(s) 365, 481
BsmFI GGGAC 1 cut(s) 504
BsnI GGCC 3 cut(s) 334, 604, 816
Bsp143I GATC 2 cut(s) 302, 724
BspACI CCGC 4 cut(s) 5, 377, 380, 515
BspANI GGCC 3 cut(s) 334, 604, 816
BspLI GGNNCC 1 cut(s) 55
BspOI GCTAGC 1 cut(s) 788
BspPI GGATC 1 cut(s) 310
BsrDI GCAATG 2 cut(s) 225, 684
BsrI ACTGG 4 cut(s) 55, 492, 745, 808
BssECI CCNNGG 2 cut(s) 298, 528
BssMI GATC 2 cut(s) 302, 724
BssT1I CCWWGG 1 cut(s) 298
Bst2UI CCWGG 1 cut(s) 708
Bst4CI ACNGT 2 cut(s) 358, 754
Bst6I CTCTTC 3 cut(s) 131, 484, 824
BstAPI GCANNNNNTGC 1 cut(s) 131
BstC8I GCNNGC 1 cut(s) 786
BstDEI CTNAG 1 cut(s) 497
BstENI CCTNNNNNAGG 1 cut(s) 527
BstF5I GGATG 2 cut(s) 574, 605
BstKTI GATC 2 cut(s) 305, 727
BstMAI GTCTC 2 cut(s) 365, 481
BstMBI GATC 2 cut(s) 302, 724
BstMWI GCNNNNNNNGC 6 cut(s) 131, 236, 502, 647, 695, 794
BstNI CCWGG 1 cut(s) 708
BstSCI CCNGG 1 cut(s) 706
BstV1I GCAGC 3 cut(s) 164, 650, 751
BstV2I GAAGAC 2 cut(s) 184, 843
BstX2I RGATCY 2 cut(s) 302, 724
BstYI RGATCY 2 cut(s) 302, 724
BsuI GTATCC 1 cut(s) 687
BsuRI GGCC 3 cut(s) 334, 604, 816
BtsCI GGATG 2 cut(s) 574, 605
BtsIMutI CAGTG 2 cut(s) 363, 485
Cac8I GCNNGC 1 cut(s) 786
Cfr13I GGNCC 2 cut(s) 332, 773
CspCI CAANNNNNGTGG 2 cut(s) 645, 680
CviAII CATG 1 cut(s) 578
DdeI CTNAG 1 cut(s) 497
DpnI GATC 2 cut(s) 304, 726
DpnII GATC 2 cut(s) 302, 724
Eam1104I CTCTTC 3 cut(s) 131, 484, 824
EarI CTCTTC 3 cut(s) 131, 484, 824
EciI GGCGGA 2 cut(s) 20, 395
Eco130I CCWWGG 1 cut(s) 298
Eco147I AGGCCT 1 cut(s) 816
Eco47I GGWCC 1 cut(s) 773
Eco57I CTGAAG 1 cut(s) 855
EcoNI CCTNNNNNAGG 1 cut(s) 527
EcoRII CCWGG 1 cut(s) 706
EcoT14I CCWWGG 1 cut(s) 298
ErhI CCWWGG 1 cut(s) 298
FaeI CATG 1 cut(s) 581
FaiI YATR 7 cut(s) 275, 326, 405, 579, 614, 654, 714
FalI AAGNNNNNCTT 4 cut(s) 627, 659, 753, 785
FaqI GGGAC 1 cut(s) 504
FatI CATG 1 cut(s) 577
Fnu4HI GCNGC 4 cut(s) 153, 378, 639, 765
FokI GGATG 2 cut(s) 581, 612
Fsp4HI GCNGC 4 cut(s) 153, 378, 639, 765
FspBI CTAG 6 cut(s) 203, 222, 506, 548, 563, 785
GluI GCNGC 4 cut(s) 153, 378, 639, 765
GsaI CCCAGC 1 cut(s) 333
GsuI CTGGAG 1 cut(s) 72
HaeIII GGCC 3 cut(s) 334, 604, 816
HapII CCGG 1 cut(s) 829
Hin1II CATG 1 cut(s) 581
HincII GTYRAC 1 cut(s) 283
HindII GTYRAC 1 cut(s) 283
HindIII AAGCTT 1 cut(s) 266
HinfI GANTC 7 cut(s) 294, 408, 581, 590, 660, 745, 839
HpaI GTTAAC 1 cut(s) 283
HpaII CCGG 1 cut(s) 829
HphI GGTGA 1 cut(s) 517
Hpy166II GTNNAC 2 cut(s) 283, 541
Hpy188I TCNGA 3 cut(s) 184, 263, 454
Hpy188III TCNNGA 1 cut(s) 563
Hpy8I GTNNAC 2 cut(s) 283, 541
HpyCH4III ACNGT 2 cut(s) 358, 754
HpyCH4V TGCA 4 cut(s) 41, 290, 361, 767
HpyF10VI GCNNNNNNNGC 6 cut(s) 131, 236, 502, 647, 695, 794
HpyF3I CTNAG 1 cut(s) 497
Hsp92II CATG 1 cut(s) 581
KspAI GTTAAC 1 cut(s) 283
Kzo9I GATC 2 cut(s) 302, 724
LmnI GCTCC 2 cut(s) 53, 463
Lsp1109I GCAGC 3 cut(s) 164, 650, 751
LweI GCATC 2 cut(s) 121, 502
MaeI CTAG 6 cut(s) 203, 222, 506, 548, 563, 785
MaeIII GTNAC 2 cut(s) 46, 208
MalI GATC 2 cut(s) 304, 726
MboI GATC 2 cut(s) 302, 724
MboII GAAGA 7 cut(s) 46, 148, 184, 471, 770, 811, 848
MflI RGATCY 2 cut(s) 302, 724
MluCI AATT 5 cut(s) 19, 89, 257, 557, 807
MlyI GAGTC 3 cut(s) 303, 669, 833
MnlI CCTC 7 cut(s) 134, 323, 523, 533, 547, 559, 827
MseI TTAA 5 cut(s) 119, 282, 345, 645, 693
MspA1I CMGCKG 2 cut(s) 155, 377
MspI CCGG 1 cut(s) 829
MspR9I CCNGG 1 cut(s) 708
MvaI CCWGG 1 cut(s) 708
MwoI GCNNNNNNNGC 6 cut(s) 131, 236, 502, 647, 695, 794
NdeII GATC 2 cut(s) 302, 724
NheI GCTAGC 1 cut(s) 784
NlaIII CATG 1 cut(s) 581
NlaIV GGNNCC 1 cut(s) 55
PceI AGGCCT 1 cut(s) 816
PfeI GAWTC 4 cut(s) 408, 581, 590, 745
PflMI CCANNNNNTGG 1 cut(s) 600
PkrI GCNGC 4 cut(s) 154, 379, 640, 766
PleI GAGTC 3 cut(s) 302, 668, 833
PpsI GAGTC 3 cut(s) 302, 668, 833
Psp6I CCWGG 1 cut(s) 706
PspFI CCCAGC 1 cut(s) 329
PspGI CCWGG 1 cut(s) 706
PspN4I GGNNCC 1 cut(s) 55
PspPI GGNCC 2 cut(s) 332, 773
PsuI RGATCY 2 cut(s) 302, 724
PvuII CAGCTG 1 cut(s) 155
SaqAI TTAA 5 cut(s) 119, 282, 345, 645, 693
SatI GCNGC 4 cut(s) 153, 378, 639, 765
Sau3AI GATC 2 cut(s) 302, 724
Sau96I GGNCC 2 cut(s) 332, 773
SchI GAGTC 3 cut(s) 303, 669, 833
ScrFI CCNGG 1 cut(s) 708
SfaNI GCATC 2 cut(s) 121, 502
SinI GGWCC 1 cut(s) 773
SmlI CTYRAG 1 cut(s) 74
SmoI CTYRAG 1 cut(s) 74
Sse9I AATT 5 cut(s) 19, 89, 257, 557, 807
SseBI AGGCCT 1 cut(s) 816
SsiI CCGC 4 cut(s) 5, 377, 380, 515
SspMI CTAG 6 cut(s) 203, 222, 506, 548, 563, 785
StuI AGGCCT 1 cut(s) 816
StyD4I CCNGG 1 cut(s) 706
StyI CCWWGG 1 cut(s) 298
TaaI ACNGT 2 cut(s) 358, 754
TaqI TCGA 2 cut(s) 584, 632
TasI AATT 5 cut(s) 19, 89, 257, 557, 807
TauI GCSGC 1 cut(s) 380
TfiI GAWTC 4 cut(s) 408, 581, 590, 745
Tru1I TTAA 5 cut(s) 119, 282, 345, 645, 693
Tru9I TTAA 5 cut(s) 119, 282, 345, 645, 693
TscAI CASTG 2 cut(s) 363, 492
TseI GCWGC 3 cut(s) 152, 638, 764
TspRI CASTG 2 cut(s) 363, 492
Van91I CCANNNNNTGG 1 cut(s) 600
VpaK11BI GGWCC 1 cut(s) 773
XagI CCTNNNNNAGG 1 cut(s) 527
XapI RAATTY 2 cut(s) 557, 807
XbaI TCTAGA 1 cut(s) 562
XspI CTAG 6 cut(s) 203, 222, 506, 548, 563, 785
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.