RLG00000003866

Salutaridine reductase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0001077
Physical Location & Seq
Reverse (-)
53725539 .. 53739899
14361 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000003866

Sequence Viewer

Length: 879 bp
ATGGCAGAAACAACCATCAGTTCCGAGTCCAAGAGGTGCTTCAATTTTTCATTTCCATTGCTTCTTATTATCATTCATCACTCGTTCTTGATTTTGATCGATTTTCAGTTTTTCTTTCCGGGTGATATTAGGATTGCAGTTGTTACTGGAGCCAACAAAGGGATTGGATTTGAGATTAGTAGGCAACTAGCTACTAAAGGAGTTGGGGTGGTAGTGACAGCCAGAGATGTGAAGAGAGGCATAGAAGCTGTTGAAAAACTTAGGGCTTCTGGTTTCCCTGATATAGTCTTTCATCTGCTAGATGTAAACAACCCAACTACCATAGCTTCTCTGGCAGATTTTCTCAAAAACCAGTTTGGAAAGCTTGACATATTGGTTAACAATGCAGCAATCCTTGGATCCACATACCTCACAGATAACATCGTACTGGACCCTGAACATTTGACGGAGGTTGGTGGCGTTGCGAGTGGCCGGGTCGGGGTCCAGAGGAGGAAGGATTTCTTAGTACTTCCACTCAGCTCGAAGGAGAGGCGCTTGGAAGCTTGTGAAGGATTTGCAGAGCAACAAGTGGACAAGATAGTTGAGGATTTTCTGGAAGATGTGAAGCAGGATTTGATAGAATCCAAACGGTGGCCTGTGTCTTCTTACATTGTATCAAAGGTAGCTCTGAATGCTTATACAAGAGTCTTGGCAAAGAAATATCCCAAAATTGCTACAAATGCAGTTAGTCCCGGTTTTACGAAAACAGACATCAACAAAAATACTGGGATTAACACAGTTGAAGAAGCTGCAGAAGGTCCTGTGAAGCTGGCTTTGATAGCTGACACTGGAATTTCTGGCTTTTACTTTGAAGAAACTGAACAGTCAACCTCTAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00902 ko01110 map00902 map01110
Pfam Domains
Protein Families
adh_short adh_short_C2

Protein Analysis

293

Amino Acids

32.07

Weight (kDa)

5.7

Isoelectric Point (pI)

39.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
adh_short PF00106 45 - 134 1.8e-18 short chain dehydrogenase
adh_short_C2 PF13561 50 - 137 3.9e-14 Enoyl-(Acyl carrier protein) reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 630
AclWI GGATC 2 cut(s) 393, 406
AcoI YGGCCR 1 cut(s) 469
AcsI RAATTY 1 cut(s) 831
AfaI GTAC 2 cut(s) 426, 507
AfiI CCNNNNNNNGG 3 cut(s) 159, 478, 630
AgsI TTSAA 4 cut(s) 43, 254, 782, 851
AjuI GAANNNNNNNTTGG 2 cut(s) 23, 55
AlwI GGATC 2 cut(s) 393, 406
AoxI GGCC 2 cut(s) 469, 632
ApeKI GCWGC 2 cut(s) 386, 788
ApoI RAATTY 1 cut(s) 831
Asp700I GAANNNNTTC 1 cut(s) 497
AspLEI GCGC 1 cut(s) 534
AspS9I GGNCC 3 cut(s) 430, 481, 797
AsuC2I CCSGG 3 cut(s) 120, 473, 732
AsuHPI GGTGA 1 cut(s) 134
AvaII GGWCC 3 cut(s) 430, 481, 797
BamHI GGATCC 1 cut(s) 398
BbsI GAAGAC 1 cut(s) 633
BbvI GCAGC 2 cut(s) 398, 775
BccI CCATC 1 cut(s) 23
BcnI CCSGG 3 cut(s) 120, 473, 732
BfaI CTAG 2 cut(s) 188, 299
BfmI CTRYAG 1 cut(s) 789
BfoI RGCGCY 1 cut(s) 535
BisI GCNGC 2 cut(s) 387, 789
BlsI GCNGC 2 cut(s) 388, 790
BmcAI AGTACT 1 cut(s) 507
Bme1390I CCNGG 3 cut(s) 120, 473, 732
Bme18I GGWCC 3 cut(s) 430, 481, 797
BmgT120I GGNCC 3 cut(s) 430, 481, 797
BmiI GGNNCC 4 cut(s) 151, 400, 432, 482
BmrFI CCNGG 3 cut(s) 120, 473, 732
BmrI ACTGGG 1 cut(s) 774
BmuI ACTGGG 1 cut(s) 774
BpiI GAAGAC 1 cut(s) 633
BpmI CTGGAG 1 cut(s) 168
BpuMI CCSGG 3 cut(s) 120, 473, 732
Bsa29I ATCGAT 1 cut(s) 99
BsaBI GATNNNNATC 1 cut(s) 95
BsaJI CCNNGG 1 cut(s) 394
BsaXI ACNNNNNCTCC 2 cut(s) 192, 222
Bsc4I CCNNNNNNNGG 3 cut(s) 159, 478, 630
Bse1I ACTGG 5 cut(s) 151, 352, 432, 769, 832
Bse3DI GCAATG 1 cut(s) 56
Bse8I GATNNNNATC 1 cut(s) 95
BseCI ATCGAT 1 cut(s) 99
BseDI CCNNGG 1 cut(s) 394
BseJI GATNNNNATC 1 cut(s) 95
BseLI CCNNNNNNNGG 3 cut(s) 159, 478, 630
BseMI GCAATG 1 cut(s) 56
BseMII CTCAG 1 cut(s) 529
BseNI ACTGG 5 cut(s) 151, 352, 432, 769, 832
BseRI GAGGAG 1 cut(s) 502
BseXI GCAGC 2 cut(s) 398, 775
BshFI GGCC 2 cut(s) 471, 634
BshVI ATCGAT 1 cut(s) 99
BsiSI CCGG 3 cut(s) 119, 472, 732
BslFI GGGAC 1 cut(s) 714
BslI CCNNNNNNNGG 3 cut(s) 159, 478, 630
BsmFI GGGAC 1 cut(s) 714
BsmI GAATGC 1 cut(s) 676
BsnI GGCC 2 cut(s) 471, 634
Bsp143I GATC 2 cut(s) 96, 398
BspANI GGCC 2 cut(s) 471, 634
BspCNI CTCAG 1 cut(s) 528
BspDI ATCGAT 1 cut(s) 99
BspLI GGNNCC 4 cut(s) 151, 400, 432, 482
BspMAI CTGCAG 1 cut(s) 793
BspPI GGATC 2 cut(s) 393, 406
BsrDI GCAATG 1 cut(s) 56
BsrI ACTGG 5 cut(s) 151, 352, 432, 769, 832
BssECI CCNNGG 1 cut(s) 394
BssMI GATC 2 cut(s) 96, 398
BssT1I CCWWGG 1 cut(s) 394
Bst4CI ACNGT 3 cut(s) 630, 778, 864
Bst6I CTCTTC 1 cut(s) 227
BstC8I GCNNGC 1 cut(s) 810
BstDEI CTNAG 3 cut(s) 260, 502, 515
BstH2I RGCGCY 1 cut(s) 535
BstHHI GCGC 1 cut(s) 534
BstKTI GATC 2 cut(s) 99, 401
BstMBI GATC 2 cut(s) 96, 398
BstMWI GCNNNNNNNGC 4 cut(s) 332, 671, 719, 818
BstSCI CCNGG 3 cut(s) 118, 471, 730
BstSFI CTRYAG 1 cut(s) 789
BstV1I GCAGC 2 cut(s) 398, 775
BstV2I GAAGAC 1 cut(s) 633
BstX2I RGATCY 1 cut(s) 398
BstYI RGATCY 1 cut(s) 398
Bsu15I ATCGAT 1 cut(s) 99
BsuRI GGCC 2 cut(s) 471, 634
BsuTUI ATCGAT 1 cut(s) 99
BtsIMutI CAGTG 1 cut(s) 825
Cac8I GCNNGC 1 cut(s) 810
CfoI GCGC 1 cut(s) 534
Cfr13I GGNCC 3 cut(s) 430, 481, 797
ClaI ATCGAT 1 cut(s) 99
Csp6I GTAC 2 cut(s) 425, 506
CviQI GTAC 2 cut(s) 425, 506
DdeI CTNAG 3 cut(s) 260, 502, 515
DpnI GATC 2 cut(s) 98, 400
DpnII GATC 2 cut(s) 96, 398
EaeI YGGCCR 1 cut(s) 469
Eam1104I CTCTTC 1 cut(s) 227
EarI CTCTTC 1 cut(s) 227
Eco130I CCWWGG 1 cut(s) 394
Eco47I GGWCC 3 cut(s) 430, 481, 797
EcoO109I RGGNCCY 1 cut(s) 797
EcoT14I CCWWGG 1 cut(s) 394
ErhI CCWWGG 1 cut(s) 394
FaiI YATR 6 cut(s) 242, 284, 323, 371, 406, 678
FalI AAGNNNNNCTT 4 cut(s) 23, 55, 485, 517
FaqI GGGAC 1 cut(s) 714
Fnu4HI GCNGC 2 cut(s) 387, 789
Fsp4HI GCNGC 2 cut(s) 387, 789
FspBI CTAG 2 cut(s) 188, 299
GlaI GCGC 1 cut(s) 533
GluI GCNGC 2 cut(s) 387, 789
GsuI CTGGAG 1 cut(s) 168
HaeII RGCGCY 1 cut(s) 535
HaeIII GGCC 2 cut(s) 471, 634
HapII CCGG 3 cut(s) 119, 472, 732
HhaI GCGC 1 cut(s) 534
Hin6I GCGC 1 cut(s) 532
HinP1I GCGC 1 cut(s) 532
HincII GTYRAC 2 cut(s) 379, 867
HindII GTYRAC 2 cut(s) 379, 867
HindIII AAGCTT 2 cut(s) 362, 540
HinfI GANTC 3 cut(s) 26, 620, 684
HpaI GTTAAC 1 cut(s) 379
HpaII CCGG 3 cut(s) 119, 472, 732
HphI GGTGA 1 cut(s) 134
Hpy166II GTNNAC 4 cut(s) 307, 379, 571, 867
Hpy188I TCNGA 2 cut(s) 25, 669
Hpy188III TCNNGA 3 cut(s) 88, 484, 593
Hpy8I GTNNAC 4 cut(s) 307, 379, 571, 867
HpyAV CCTTC 4 cut(s) 487, 517, 542, 788
HpyCH4III ACNGT 3 cut(s) 630, 778, 864
HpyCH4V TGCA 5 cut(s) 137, 386, 557, 722, 791
HpyF10VI GCNNNNNNNGC 4 cut(s) 332, 671, 719, 818
HpyF3I CTNAG 3 cut(s) 260, 502, 515
HspAI GCGC 1 cut(s) 532
KspAI GTTAAC 1 cut(s) 379
Kzo9I GATC 2 cut(s) 96, 398
LmnI GCTCC 1 cut(s) 149
Lsp1109I GCAGC 2 cut(s) 398, 775
MaeI CTAG 2 cut(s) 188, 299
MaeIII GTNAC 2 cut(s) 142, 214
MalI GATC 2 cut(s) 98, 400
MboI GATC 2 cut(s) 96, 398
MboII GAAGA 5 cut(s) 244, 608, 633, 794, 863
MflI RGATCY 1 cut(s) 398
MluCI AATT 4 cut(s) 43, 708, 831, 874
MlyI GAGTC 2 cut(s) 35, 693
MnlI CCTC 8 cut(s) 27, 230, 419, 442, 480, 483, 522, 577
MroXI GAANNNNTTC 1 cut(s) 497
MseI TTAA 2 cut(s) 378, 771
MspI CCGG 3 cut(s) 119, 472, 732
MspR9I CCNGG 3 cut(s) 120, 473, 732
Mva1269I GAATGC 1 cut(s) 676
MwoI GCNNNNNNNGC 4 cut(s) 332, 671, 719, 818
NciI CCSGG 3 cut(s) 120, 473, 732
NdeII GATC 2 cut(s) 96, 398
NlaIV GGNNCC 4 cut(s) 151, 400, 432, 482
NmuCI GTSAC 1 cut(s) 214
PctI GAATGC 1 cut(s) 676
PdmI GAANNNNTTC 1 cut(s) 497
PfeI GAWTC 1 cut(s) 620
PflMI CCANNNNNTGG 1 cut(s) 630
PkrI GCNGC 2 cut(s) 388, 790
PleI GAGTC 2 cut(s) 34, 692
PpsI GAGTC 2 cut(s) 34, 692
PpuMI RGGWCCY 1 cut(s) 797
Psp5II RGGWCCY 1 cut(s) 797
PspN4I GGNNCC 4 cut(s) 151, 400, 432, 482
PspPI GGNCC 3 cut(s) 430, 481, 797
PspPPI RGGWCCY 1 cut(s) 797
PstI CTGCAG 1 cut(s) 793
PsuI RGATCY 1 cut(s) 398
RsaI GTAC 2 cut(s) 426, 507
RsaNI GTAC 2 cut(s) 425, 506
SaqAI TTAA 2 cut(s) 378, 771
SatI GCNGC 2 cut(s) 387, 789
Sau3AI GATC 2 cut(s) 96, 398
Sau96I GGNCC 3 cut(s) 430, 481, 797
ScaI AGTACT 1 cut(s) 507
SchI GAGTC 2 cut(s) 35, 693
ScrFI CCNGG 3 cut(s) 120, 473, 732
SfcI CTRYAG 1 cut(s) 789
SinI GGWCC 3 cut(s) 430, 481, 797
Sse9I AATT 4 cut(s) 43, 708, 831, 874
SspMI CTAG 2 cut(s) 188, 299
StyD4I CCNGG 3 cut(s) 118, 471, 730
StyI CCWWGG 1 cut(s) 394
TaaI ACNGT 3 cut(s) 630, 778, 864
TaqI TCGA 2 cut(s) 99, 521
TasI AATT 4 cut(s) 43, 708, 831, 874
TatI WGTACW 1 cut(s) 505
TfiI GAWTC 1 cut(s) 620
Tru1I TTAA 2 cut(s) 378, 771
Tru9I TTAA 2 cut(s) 378, 771
TscAI CASTG 1 cut(s) 832
TseFI GTSAC 1 cut(s) 214
TseI GCWGC 2 cut(s) 386, 788
Tsp45I GTSAC 1 cut(s) 214
TspDTI ATGAA 3 cut(s) 39, 65, 281
TspGWI ACGGA 1 cut(s) 461
TspRI CASTG 1 cut(s) 832
Van91I CCANNNNNTGG 1 cut(s) 630
VpaK11BI GGWCC 3 cut(s) 430, 481, 797
XapI RAATTY 1 cut(s) 831
XcmI CCANNNNNNNNNTGG 1 cut(s) 328
XmnI GAANNNNTTC 1 cut(s) 497
XspI CTAG 2 cut(s) 188, 299
ZrmI AGTACT 1 cut(s) 507
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.