RchiOBHm_Chr7g0198571

Salutaridine reductase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0001077
Physical Location & Seq
Reverse (-)
16509654 .. 16511402
1749 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ17766

Sequence Viewer

Length: 792 bp
ATGGCGGAAACTACCATCAATTTTGGGTCAAAGAAGATTGCAGTTGTTACTGGAGCCAACAAAGGGATTGGACTTGAGATTAGTAAGCAATTAGCTTCTAAAGGAGTTGGGGTGGTATTAACAGCAAGAGATGCGAAGAGAGGAACAGAAGCAGCTGAAAGCCTAAAGGCTTCTGGTTTCTCCGATGTGGTCTTCCATCAGCTAGATGTAACTGCCCCAACTAGCATTGCTTCTTTGGCAAACTTTCTCAAAACGCAATTCAGAAAGCTTGACATATTGGTTAAACAAAATACAGTGCAAACTTACGAGACAGCGGCGGATTGTTTGAAAACAAACTATTATGGAATCAAGCAACTCACAGAAGCACTTGTTCCACTACTTCAAAAATCAGAAGCTCCAAAGATAGTCAATGTCTCTTCCACACTGGGACAGCTAAGAGAGCTAGGAGATGCGGATAACCTCACCGAGGAGAAAGTGGACAAGCTAGTTGAGGAATTTCTAGAGGATGTGAAGCATGATTCGATAGAATCCAAAGGATGGCCTTTAGATATATCTGCCTACATTGTATCGAAAGCAGCTCTTAATGCTTATACAAGAGTCGTGGCAAAGAAGTATCCCCACATTGCTATTAACGCAGTTAGTCCAGGTTATACCAAAACAGATCTCAACAATAATACTGGGATTCTCACAGTTGAAGAAGCTGCACTTGGTCCTGTGAAGCTAGCTTTGATAGCCGAAACTGGAATTTCAGGCCTCTTCTTTGACCGGACTGAAGACTCAACCTTTGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00902 ko01110 map00902 map01110
Pfam Domains
Protein Families
adh_short adh_short_C2

Protein Analysis

263

Amino Acids

28.34

Weight (kDa)

5.55

Isoelectric Point (pI)

27.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
adh_short PF00106 12 - 145 2.5e-24 short chain dehydrogenase
KR PF08659 15 - 100 3.7e-06 KR domain
adh_short_C2 PF13561 18 - 144 7.1e-15 Enoyl-(Acyl carrier protein) reductase
adh_short_C2 PF13561 183 - 225 1.5e-06 Enoyl-(Acyl carrier protein) reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 537
AciI CCGC 4 cut(s) 5, 314, 317, 452
AcsI RAATTY 2 cut(s) 494, 744
AcuI CTGAAG 1 cut(s) 792
AfiI CCNNNNNNNGG 3 cut(s) 63, 466, 537
AgsI TTSAA 3 cut(s) 328, 383, 695
AjnI CCWGG 1 cut(s) 643
AjuI GAANNNNNNNTTGG 2 cut(s) 690, 722
Alw26I GTCTC 2 cut(s) 302, 418
AoxI GGCC 2 cut(s) 539, 751
ApeKI GCWGC 3 cut(s) 152, 575, 701
ApoI RAATTY 2 cut(s) 494, 744
ArsI GACNNNNNNTTYG 1 cut(s) 767
AspS9I GGNCC 1 cut(s) 710
AsuHPI GGTGA 1 cut(s) 454
AsuNHI GCTAGC 1 cut(s) 721
AvaII GGWCC 1 cut(s) 710
BbsI GAAGAC 2 cut(s) 184, 780
BbvI GCAGC 3 cut(s) 164, 587, 688
BccI CCATC 3 cut(s) 23, 204, 531
BciT130I CCWGG 1 cut(s) 645
BciVI GTATCC 1 cut(s) 624
BcoDI GTCTC 2 cut(s) 302, 418
BfaI CTAG 6 cut(s) 203, 222, 443, 485, 500, 722
BfuI GTATCC 1 cut(s) 624
BglII AGATCT 1 cut(s) 661
BisI GCNGC 4 cut(s) 153, 315, 576, 702
BlsI GCNGC 4 cut(s) 154, 316, 577, 703
Bme1390I CCNGG 1 cut(s) 645
Bme18I GGWCC 1 cut(s) 710
BmgT120I GGNCC 1 cut(s) 710
BmiI GGNNCC 1 cut(s) 55
BmrFI CCNGG 1 cut(s) 645
BmrI ACTGGG 2 cut(s) 434, 687
BmsI GCATC 2 cut(s) 121, 439
BmtI GCTAGC 1 cut(s) 725
BmuI ACTGGG 2 cut(s) 434, 687
BpiI GAAGAC 2 cut(s) 184, 780
BpmI CTGGAG 1 cut(s) 72
BpuEI CTTGAG 1 cut(s) 95
BsaJI CCNNGG 1 cut(s) 465
BsaWI WCCGGW 1 cut(s) 765
BsaXI ACNNNNNCTCC 2 cut(s) 96, 126
Bsc4I CCNNNNNNNGG 3 cut(s) 63, 466, 537
Bse1I ACTGG 4 cut(s) 55, 429, 682, 745
Bse3DI GCAATG 2 cut(s) 225, 621
BseBI CCWGG 1 cut(s) 645
BseDI CCNNGG 1 cut(s) 465
BseGI GGATG 2 cut(s) 511, 542
BseLI CCNNNNNNNGG 3 cut(s) 63, 466, 537
BseMI GCAATG 2 cut(s) 225, 621
BseNI ACTGG 4 cut(s) 55, 429, 682, 745
BseRI GAGGAG 1 cut(s) 482
BseXI GCAGC 3 cut(s) 164, 587, 688
BsgI GTGCAG 1 cut(s) 687
BshFI GGCC 2 cut(s) 541, 753
BsiSI CCGG 1 cut(s) 766
BslFI GGGAC 1 cut(s) 441
BslI CCNNNNNNNGG 3 cut(s) 63, 466, 537
BsmAI GTCTC 2 cut(s) 302, 418
BsmFI GGGAC 1 cut(s) 441
BsnI GGCC 2 cut(s) 541, 753
Bsp143I GATC 1 cut(s) 661
BspACI CCGC 4 cut(s) 5, 314, 317, 452
BspANI GGCC 2 cut(s) 541, 753
BspLI GGNNCC 1 cut(s) 55
BspOI GCTAGC 1 cut(s) 725
BsrDI GCAATG 2 cut(s) 225, 621
BsrI ACTGG 4 cut(s) 55, 429, 682, 745
BssECI CCNNGG 1 cut(s) 465
BssMI GATC 1 cut(s) 661
Bst2UI CCWGG 1 cut(s) 645
Bst4CI ACNGT 2 cut(s) 295, 691
Bst6I CTCTTC 3 cut(s) 131, 421, 761
BstAPI GCANNNNNTGC 1 cut(s) 131
BstC8I GCNNGC 1 cut(s) 723
BstDEI CTNAG 1 cut(s) 434
BstENI CCTNNNNNAGG 1 cut(s) 464
BstF5I GGATG 2 cut(s) 511, 542
BstKTI GATC 1 cut(s) 664
BstMAI GTCTC 2 cut(s) 302, 418
BstMBI GATC 1 cut(s) 661
BstMWI GCNNNNNNNGC 6 cut(s) 131, 236, 439, 584, 632, 731
BstNI CCWGG 1 cut(s) 645
BstSCI CCNGG 1 cut(s) 643
BstV1I GCAGC 3 cut(s) 164, 587, 688
BstV2I GAAGAC 2 cut(s) 184, 780
BstX2I RGATCY 1 cut(s) 661
BstYI RGATCY 1 cut(s) 661
BsuI GTATCC 1 cut(s) 624
BsuRI GGCC 2 cut(s) 541, 753
BtsCI GGATG 2 cut(s) 511, 542
BtsIMutI CAGTG 2 cut(s) 300, 422
Cac8I GCNNGC 1 cut(s) 723
Cfr13I GGNCC 1 cut(s) 710
CspCI CAANNNNNGTGG 2 cut(s) 582, 617
CviAII CATG 1 cut(s) 515
DdeI CTNAG 1 cut(s) 434
DpnI GATC 1 cut(s) 663
DpnII GATC 1 cut(s) 661
Eam1104I CTCTTC 3 cut(s) 131, 421, 761
EarI CTCTTC 3 cut(s) 131, 421, 761
EciI GGCGGA 2 cut(s) 20, 332
Eco147I AGGCCT 1 cut(s) 753
Eco47I GGWCC 1 cut(s) 710
Eco57I CTGAAG 1 cut(s) 792
EcoNI CCTNNNNNAGG 1 cut(s) 464
EcoRII CCWGG 1 cut(s) 643
FaeI CATG 1 cut(s) 518
FaiI YATR 6 cut(s) 275, 342, 516, 551, 591, 651
FalI AAGNNNNNCTT 4 cut(s) 564, 596, 690, 722
FaqI GGGAC 1 cut(s) 441
FatI CATG 1 cut(s) 514
Fnu4HI GCNGC 4 cut(s) 153, 315, 576, 702
FokI GGATG 2 cut(s) 518, 549
Fsp4HI GCNGC 4 cut(s) 153, 315, 576, 702
FspBI CTAG 6 cut(s) 203, 222, 443, 485, 500, 722
GluI GCNGC 4 cut(s) 153, 315, 576, 702
GsuI CTGGAG 1 cut(s) 72
HaeIII GGCC 2 cut(s) 541, 753
HapII CCGG 1 cut(s) 766
Hin1II CATG 1 cut(s) 518
HindIII AAGCTT 1 cut(s) 266
HinfI GANTC 6 cut(s) 345, 518, 527, 597, 682, 776
HpaII CCGG 1 cut(s) 766
HphI GGTGA 1 cut(s) 454
Hpy166II GTNNAC 1 cut(s) 478
Hpy188I TCNGA 3 cut(s) 184, 263, 391
Hpy188III TCNNGA 1 cut(s) 500
Hpy8I GTNNAC 1 cut(s) 478
HpyCH4III ACNGT 2 cut(s) 295, 691
HpyCH4V TGCA 3 cut(s) 41, 298, 704
HpyF10VI GCNNNNNNNGC 6 cut(s) 131, 236, 439, 584, 632, 731
HpyF3I CTNAG 1 cut(s) 434
Hsp92II CATG 1 cut(s) 518
Kzo9I GATC 1 cut(s) 661
LmnI GCTCC 2 cut(s) 53, 400
Lsp1109I GCAGC 3 cut(s) 164, 587, 688
LweI GCATC 2 cut(s) 121, 439
MaeI CTAG 6 cut(s) 203, 222, 443, 485, 500, 722
MaeIII GTNAC 2 cut(s) 46, 208
MalI GATC 1 cut(s) 663
MboI GATC 1 cut(s) 661
MboII GAAGA 7 cut(s) 46, 148, 184, 408, 707, 748, 785
MflI RGATCY 1 cut(s) 661
MluCI AATT 5 cut(s) 19, 89, 257, 494, 744
MlyI GAGTC 2 cut(s) 606, 770
MnlI CCTC 6 cut(s) 134, 460, 470, 484, 496, 764
MseI TTAA 4 cut(s) 119, 282, 582, 630
MspA1I CMGCKG 2 cut(s) 155, 314
MspI CCGG 1 cut(s) 766
MspR9I CCNGG 1 cut(s) 645
MvaI CCWGG 1 cut(s) 645
MwoI GCNNNNNNNGC 6 cut(s) 131, 236, 439, 584, 632, 731
NdeII GATC 1 cut(s) 661
NheI GCTAGC 1 cut(s) 721
NlaIII CATG 1 cut(s) 518
NlaIV GGNNCC 1 cut(s) 55
PceI AGGCCT 1 cut(s) 753
PfeI GAWTC 4 cut(s) 345, 518, 527, 682
PflMI CCANNNNNTGG 1 cut(s) 537
PkrI GCNGC 4 cut(s) 154, 316, 577, 703
PleI GAGTC 2 cut(s) 605, 770
PpsI GAGTC 2 cut(s) 605, 770
Psp6I CCWGG 1 cut(s) 643
PspGI CCWGG 1 cut(s) 643
PspN4I GGNNCC 1 cut(s) 55
PspPI GGNCC 1 cut(s) 710
PsuI RGATCY 1 cut(s) 661
PvuII CAGCTG 1 cut(s) 155
SaqAI TTAA 4 cut(s) 119, 282, 582, 630
SatI GCNGC 4 cut(s) 153, 315, 576, 702
Sau3AI GATC 1 cut(s) 661
Sau96I GGNCC 1 cut(s) 710
SchI GAGTC 2 cut(s) 606, 770
ScrFI CCNGG 1 cut(s) 645
SfaNI GCATC 2 cut(s) 121, 439
SinI GGWCC 1 cut(s) 710
SmlI CTYRAG 1 cut(s) 74
SmoI CTYRAG 1 cut(s) 74
Sse9I AATT 5 cut(s) 19, 89, 257, 494, 744
SseBI AGGCCT 1 cut(s) 753
SsiI CCGC 4 cut(s) 5, 314, 317, 452
SspMI CTAG 6 cut(s) 203, 222, 443, 485, 500, 722
StuI AGGCCT 1 cut(s) 753
StyD4I CCNGG 1 cut(s) 643
TaaI ACNGT 2 cut(s) 295, 691
TaqI TCGA 2 cut(s) 521, 569
TasI AATT 5 cut(s) 19, 89, 257, 494, 744
TauI GCSGC 1 cut(s) 317
TfiI GAWTC 4 cut(s) 345, 518, 527, 682
Tru1I TTAA 4 cut(s) 119, 282, 582, 630
Tru9I TTAA 4 cut(s) 119, 282, 582, 630
TscAI CASTG 2 cut(s) 300, 429
TseI GCWGC 3 cut(s) 152, 575, 701
TspRI CASTG 2 cut(s) 300, 429
Van91I CCANNNNNTGG 1 cut(s) 537
VpaK11BI GGWCC 1 cut(s) 710
XagI CCTNNNNNAGG 1 cut(s) 464
XapI RAATTY 2 cut(s) 494, 744
XbaI TCTAGA 1 cut(s) 499
XspI CTAG 6 cut(s) 203, 222, 443, 485, 500, 722
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.