FvH4_5g01930

Salutaridine reductase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0001077
Physical Location & Seq
Forward (+)
1205679 .. 1207566
1888 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g01930.t1

Sequence Viewer

Length: 858 bp
ATGGAGGAAACTACCATCAATTTTGGGTCTAAGAAGATTGCAGTTGTTACTGGAGCCAACAAAGGGATTGGACTTGAGATTAGCAAGCAATTAGCTGCTAAAGGAGTTGGGGTGGTATTAACAGCAAGAGATGTGAAGAGAGGAACAGAAGCTGCTGAAAATCTTAAGGCTTCTGGGTTCTCTGATGTGGTATTTCATCAGCTAGATGTAACAGAGCCGACTACTATTGCTTCTTTGGCAAACTTTCTTGAAACGCAATTTGGAAAGCTTGACATATTGGTTAACAATGCAGGAGTCGTTGGATCTGTATACCTCACAGCCGACTATGATCCAGTGCAAACATACGAGACAGCGAGGGATTGTTTGAAAACAAACTATTATGGGCTCAAGCAACTCACAGAAGCACTTGTTCCGCTGCTTCAAAAATCTGAAGCTGCAAGGATAGTCAATGTCTCTTCCGGATTAGGACAGCTAAGAAATATTGGAAATGAGAAGGCCAAGAAGGAGCTAGGAGATGCAGATAACCTCAACGAGGAGAAAGTGGACAAGCTAGTTGAGGAATTTCTGGAGGATGTGAAACAGGATTCGATAGAATCGAAAGGCTGGCCTCTAAGTATATCTGCCTACATTGTCTCAAAAGCAGCTCTGAATGCTTATACAAGACTCTTGGCAAAGAAGTATCCCCATATTGCCATAAACGCAGTTGGTCCAGGTTATACCAAAACAGACCTCAATAATAATTCCGGGATTCTCACAGTTGAAGAAGCTGCAGTAGGTCCTGTGAGGCTGGCTTTGATAGCCGAAACTAGAATTTCCGGCCTCTTCTTCAACAGAAATGAAGAGTCGACCTTTGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00902 ko01110 map00902 map01110
Pfam Domains
Protein Families
adh_short adh_short_C2

Protein Analysis

286

Amino Acids

30.8

Weight (kDa)

5.23

Isoelectric Point (pI)

29.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
adh_short PF00106 12 - 162 8.4e-31 short chain dehydrogenase
KR PF08659 15 - 162 8.6e-09 KR domain
adh_short_C2 PF13561 18 - 156 2.9e-22 Enoyl-(Acyl carrier protein) reductase
adh_short_C2 PF13561 200 - 247 7.5e-06 Enoyl-(Acyl carrier protein) reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 309, 845
AccIII TCCGGA 1 cut(s) 458
AciI CCGC 1 cut(s) 413
AclWI GGATC 2 cut(s) 310, 323
AcsI RAATTY 2 cut(s) 560, 810
AcuI CTGAAG 1 cut(s) 450
AfiI CCNNNNNNNGG 2 cut(s) 63, 532
AflII CTTAAG 1 cut(s) 164
AgsI TTSAA 5 cut(s) 251, 367, 422, 761, 829
AjnI CCWGG 1 cut(s) 709
AjuI GAANNNNNNNTTGG 2 cut(s) 243, 275
Alw26I GTCTC 3 cut(s) 341, 457, 637
AlwI GGATC 2 cut(s) 310, 323
AlwNI CAGNNNCTG 1 cut(s) 152
Aor13HI TCCGGA 1 cut(s) 458
AoxI GGCC 3 cut(s) 495, 605, 817
ApeKI GCWGC 6 cut(s) 95, 152, 415, 434, 641, 767
ApoI RAATTY 2 cut(s) 560, 810
AspS9I GGNCC 2 cut(s) 707, 776
AsuC2I CCSGG 1 cut(s) 745
AvaII GGWCC 2 cut(s) 707, 776
BanII GRGCYC 1 cut(s) 387
BbvI GCAGC 6 cut(s) 82, 139, 402, 421, 653, 754
BccI CCATC 1 cut(s) 23
BciT130I CCWGG 1 cut(s) 711
BciVI GTATCC 1 cut(s) 690
BcnI CCSGG 1 cut(s) 745
BcoDI GTCTC 3 cut(s) 341, 457, 637
BfaI CTAG 4 cut(s) 203, 509, 551, 807
BfmI CTRYAG 1 cut(s) 768
BfrI CTTAAG 1 cut(s) 164
BfuI GTATCC 1 cut(s) 690
BisI GCNGC 6 cut(s) 96, 153, 416, 435, 642, 768
BlsI GCNGC 6 cut(s) 97, 154, 417, 436, 643, 769
Bme1390I CCNGG 2 cut(s) 711, 745
Bme18I GGWCC 2 cut(s) 707, 776
BmgT120I GGNCC 2 cut(s) 707, 776
BmiI GGNNCC 1 cut(s) 55
BmrFI CCNGG 2 cut(s) 711, 745
BmsI GCATC 1 cut(s) 505
BpmI CTGGAG 2 cut(s) 72, 587
BpuEI CTTGAG 2 cut(s) 95, 371
BpuMI CCSGG 1 cut(s) 745
BsaWI WCCGGW 1 cut(s) 458
BsaXI ACNNNNNCTCC 2 cut(s) 96, 126
Bsc4I CCNNNNNNNGG 2 cut(s) 63, 532
Bse1I ACTGG 2 cut(s) 55, 332
BseAI TCCGGA 1 cut(s) 458
BseBI CCWGG 1 cut(s) 711
BseGI GGATG 1 cut(s) 577
BseLI CCNNNNNNNGG 2 cut(s) 63, 532
BseNI ACTGG 2 cut(s) 55, 332
BseRI GAGGAG 1 cut(s) 548
BseXI GCAGC 6 cut(s) 82, 139, 402, 421, 653, 754
BshFI GGCC 3 cut(s) 497, 607, 819
BsiSI CCGG 3 cut(s) 459, 744, 816
BslI CCNNNNNNNGG 2 cut(s) 63, 532
BsmAI GTCTC 3 cut(s) 341, 457, 637
BsmI GAATGC 1 cut(s) 655
BsnI GGCC 3 cut(s) 497, 607, 819
Bsp1286I GDGCHC 1 cut(s) 387
Bsp13I TCCGGA 1 cut(s) 458
Bsp143I GATC 2 cut(s) 302, 328
BspACI CCGC 1 cut(s) 413
BspANI GGCC 3 cut(s) 497, 607, 819
BspEI TCCGGA 1 cut(s) 458
BspLI GGNNCC 1 cut(s) 55
BspMAI CTGCAG 1 cut(s) 772
BspPI GGATC 2 cut(s) 310, 323
BspTI CTTAAG 1 cut(s) 164
BsrI ACTGG 2 cut(s) 55, 332
BssMI GATC 2 cut(s) 302, 328
BssNAI GTATAC 1 cut(s) 310
Bst1107I GTATAC 1 cut(s) 310
Bst2UI CCWGG 1 cut(s) 711
Bst4CI ACNGT 1 cut(s) 757
Bst6I CTCTTC 4 cut(s) 131, 460, 827, 834
BstAFI CTTAAG 1 cut(s) 164
BstC8I GCNNGC 3 cut(s) 86, 605, 789
BstDEI CTNAG 3 cut(s) 30, 473, 611
BstENI CCTNNNNNAGG 1 cut(s) 530
BstF5I GGATG 1 cut(s) 577
BstKTI GATC 2 cut(s) 305, 331
BstMAI GTCTC 3 cut(s) 341, 457, 637
BstMBI GATC 2 cut(s) 302, 328
BstMWI GCNNNNNNNGC 4 cut(s) 236, 650, 698, 797
BstNI CCWGG 1 cut(s) 711
BstSCI CCNGG 2 cut(s) 709, 743
BstSFI CTRYAG 1 cut(s) 768
BstV1I GCAGC 6 cut(s) 82, 139, 402, 421, 653, 754
BstX2I RGATCY 1 cut(s) 302
BstYI RGATCY 1 cut(s) 302
BstZ17I GTATAC 1 cut(s) 310
BsuI GTATCC 1 cut(s) 690
BsuRI GGCC 3 cut(s) 497, 607, 819
BtsCI GGATG 1 cut(s) 577
BtsIMutI CAGTG 1 cut(s) 339
Cac8I GCNNGC 3 cut(s) 86, 605, 789
CaiI CAGNNNCTG 1 cut(s) 152
Cfr13I GGNCC 2 cut(s) 707, 776
DdeI CTNAG 3 cut(s) 30, 473, 611
DpnI GATC 2 cut(s) 304, 330
DpnII GATC 2 cut(s) 302, 328
Eam1104I CTCTTC 4 cut(s) 131, 460, 827, 834
EarI CTCTTC 4 cut(s) 131, 460, 827, 834
Eco24I GRGCYC 1 cut(s) 387
Eco47I GGWCC 2 cut(s) 707, 776
Eco57I CTGAAG 1 cut(s) 450
EcoNI CCTNNNNNAGG 1 cut(s) 530
EcoO109I RGGNCCY 1 cut(s) 776
EcoRII CCWGG 1 cut(s) 709
EcoT38I GRGCYC 1 cut(s) 387
FblI GTMKAC 2 cut(s) 309, 845
Fnu4HI GCNGC 6 cut(s) 96, 153, 416, 435, 642, 768
FokI GGATG 1 cut(s) 584
FriOI GRGCYC 1 cut(s) 387
Fsp4HI GCNGC 6 cut(s) 96, 153, 416, 435, 642, 768
FspBI CTAG 4 cut(s) 203, 509, 551, 807
GluI GCNGC 6 cut(s) 96, 153, 416, 435, 642, 768
GsuI CTGGAG 2 cut(s) 72, 587
HaeIII GGCC 3 cut(s) 497, 607, 819
HapII CCGG 3 cut(s) 459, 744, 816
HincII GTYRAC 2 cut(s) 283, 846
HindII GTYRAC 2 cut(s) 283, 846
HindIII AAGCTT 1 cut(s) 266
HinfI GANTC 6 cut(s) 294, 584, 593, 663, 748, 842
HpaI GTTAAC 1 cut(s) 283
HpaII CCGG 3 cut(s) 459, 744, 816
Hpy166II GTNNAC 4 cut(s) 283, 310, 544, 846
Hpy188I TCNGA 3 cut(s) 184, 430, 648
Hpy188III TCNNGA 3 cut(s) 248, 459, 566
Hpy8I GTNNAC 4 cut(s) 283, 310, 544, 846
HpyAV CCTTC 2 cut(s) 487, 496
HpyCH4III ACNGT 1 cut(s) 757
HpyCH4V TGCA 6 cut(s) 41, 290, 337, 437, 518, 770
HpyF10VI GCNNNNNNNGC 4 cut(s) 236, 650, 698, 797
HpyF3I CTNAG 3 cut(s) 30, 473, 611
Kpn2I TCCGGA 1 cut(s) 458
KspAI GTTAAC 1 cut(s) 283
Kzo9I GATC 2 cut(s) 302, 328
LmnI GCTCC 2 cut(s) 53, 505
Lsp1109I GCAGC 6 cut(s) 82, 139, 402, 421, 653, 754
LweI GCATC 1 cut(s) 505
MaeI CTAG 4 cut(s) 203, 509, 551, 807
MaeIII GTNAC 2 cut(s) 46, 208
MalI GATC 2 cut(s) 304, 330
MboI GATC 2 cut(s) 302, 328
MboII GAAGA 7 cut(s) 46, 148, 447, 773, 814, 817, 851
MflI RGATCY 1 cut(s) 302
MhlI GDGCHC 1 cut(s) 387
MluCI AATT 6 cut(s) 19, 89, 257, 560, 739, 810
MlyI GAGTC 3 cut(s) 303, 657, 851
MmeI TCCRAC 1 cut(s) 280
MroI TCCGGA 1 cut(s) 458
MseI TTAA 3 cut(s) 119, 165, 282
MspA1I CMGCKG 1 cut(s) 415
MspCI CTTAAG 1 cut(s) 164
MspI CCGG 3 cut(s) 459, 744, 816
MspR9I CCNGG 2 cut(s) 711, 745
Mva1269I GAATGC 1 cut(s) 655
MvaI CCWGG 1 cut(s) 711
MwoI GCNNNNNNNGC 4 cut(s) 236, 650, 698, 797
NciI CCSGG 1 cut(s) 745
NdeII GATC 2 cut(s) 302, 328
NlaIV GGNNCC 1 cut(s) 55
PcsI WCGNNNNNNNCGW 1 cut(s) 593
PctI GAATGC 1 cut(s) 655
PfeI GAWTC 3 cut(s) 584, 593, 748
PfoI TCCNGGA 1 cut(s) 743
PkrI GCNGC 6 cut(s) 97, 154, 417, 436, 643, 769
PleI GAGTC 3 cut(s) 302, 657, 850
PpsI GAGTC 3 cut(s) 302, 657, 850
PpuMI RGGWCCY 1 cut(s) 776
Psp5II RGGWCCY 1 cut(s) 776
Psp6I CCWGG 1 cut(s) 709
PspGI CCWGG 1 cut(s) 709
PspN4I GGNNCC 1 cut(s) 55
PspPI GGNCC 2 cut(s) 707, 776
PspPPI RGGWCCY 1 cut(s) 776
PstI CTGCAG 1 cut(s) 772
PstNI CAGNNNCTG 1 cut(s) 152
PsuI RGATCY 1 cut(s) 302
SalI GTCGAC 1 cut(s) 844
SaqAI TTAA 3 cut(s) 119, 165, 282
SatI GCNGC 6 cut(s) 96, 153, 416, 435, 642, 768
Sau3AI GATC 2 cut(s) 302, 328
Sau96I GGNCC 2 cut(s) 707, 776
SchI GAGTC 3 cut(s) 303, 657, 851
ScrFI CCNGG 2 cut(s) 711, 745
SduI GDGCHC 1 cut(s) 387
SfaNI GCATC 1 cut(s) 505
SfcI CTRYAG 1 cut(s) 768
SinI GGWCC 2 cut(s) 707, 776
SmlI CTYRAG 3 cut(s) 74, 164, 386
SmoI CTYRAG 3 cut(s) 74, 164, 386
Sse9I AATT 6 cut(s) 19, 89, 257, 560, 739, 810
SsiI CCGC 1 cut(s) 413
SspI AATATT 1 cut(s) 481
SspMI CTAG 4 cut(s) 203, 509, 551, 807
StyD4I CCNGG 2 cut(s) 709, 743
TaaI ACNGT 1 cut(s) 757
TaqI TCGA 3 cut(s) 587, 596, 845
TasI AATT 6 cut(s) 19, 89, 257, 560, 739, 810
TfiI GAWTC 3 cut(s) 584, 593, 748
Tru1I TTAA 3 cut(s) 119, 165, 282
Tru9I TTAA 3 cut(s) 119, 165, 282
TscAI CASTG 1 cut(s) 339
TseI GCWGC 6 cut(s) 95, 152, 415, 434, 641, 767
TspDTI ATGAA 2 cut(s) 185, 852
TspRI CASTG 1 cut(s) 339
Vha464I CTTAAG 1 cut(s) 164
VpaK11BI GGWCC 2 cut(s) 707, 776
XagI CCTNNNNNAGG 1 cut(s) 530
XapI RAATTY 2 cut(s) 560, 810
XmiI GTMKAC 2 cut(s) 309, 845
XspI CTAG 4 cut(s) 203, 509, 551, 807
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.