RchiOBHm_Chr7g0211971

Salutaridine reductase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0001077
Physical Location & Seq
Forward (+)
29149411 .. 29151139
1729 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ18964

Sequence Viewer

Length: 903 bp
ATGGCAGAAACAACCATCAGTTTTGAGTCCAAGAGGATTGCTGTTGTTACTGGAGCCAACAAAGGGATTGGACTTGAGATTAGTAGGCAATTAGCTTCTAATGGAGTTGGGGTGGTACTAACAGCAAGAGATTTGAAGAGAGGCACAGAAGCTGTTGAAAAGCTTAAGGCCTCTGGTTTCTCTGATGTGGTCTTTCATCAGCTAGATGTAAATAACCCAACCACCATTGCTGCTCTGGCAGATTTTCTCAAAACCCAGTTTGGAAAGCTTGACATTTTGGTTAATAATGCAGGAGTCCTTGGATCCACATACCTCACCGATAACGTCGTAGTGGAGCCTCAAGATATCTTAGGTTCTACATCACTGAAAGAAGTTTTAGTGCAAACATATGAGACGGCAGAGGATTGCTTGAAAACAAACTATTATGGAATCAAGCAACTCACAGAAGCACTTCTTCCGCTTCTTCAAAAATCAGAAGCAGCAAGGATAGTCAATGTCTCTTCAGCACTGGGACAGCTAAGGGCTATTGCAAATGAGAAAGCCAAGAAGGAGCTAGGAGATGTCAATAACCTCACCGAGGAAAAAGTGGACAAGCTGGTTGAGGAATTTCTGGAGGATGTGAAGCAGGATTTGATAGAATCCAAAGGCTGGCCGCTAAACATGTCTTCTTACATTGTATCAAAAGCAGCTCTGAATGCTTATACAAGAGTCTTGGCCAGGAAGTATCCCAAAATTGCTACAAACGCAGTTAGTCCTGGTTTTACCAAAACAGACATCAACCAAAATACCGGGATTAACACAGTTGAAGAAGCTGCAGAAGGTCCTGTGAAGCTGGCTTTAATAGCTGACTCTGGTATTTCTGGCCTCTACTTTGAAATGACGGAAGAGTCATCCTTTGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00902 ko01110 map00902 map01110
Pfam Domains
Protein Families
adh_short adh_short_C2

Protein Analysis

300

Amino Acids

32.44

Weight (kDa)

4.94

Isoelectric Point (pI)

30.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
adh_short PF00106 13 - 174 7.5e-29 short chain dehydrogenase
KR PF08659 15 - 107 5.6e-08 KR domain
adh_short_C2 PF13561 18 - 178 4e-19 Enoyl-(Acyl carrier protein) reductase
adh_short_C2 PF13561 217 - 267 4.6e-07 Enoyl-(Acyl carrier protein) reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 886
AccB7I CCANNNNNTGG 1 cut(s) 648
AciI CCGC 2 cut(s) 458, 653
AclWI GGATC 2 cut(s) 297, 310
AcoI YGGCCR 2 cut(s) 650, 714
AcsI RAATTY 1 cut(s) 605
AcuI CTGAAG 1 cut(s) 486
AfaI GTAC 1 cut(s) 117
AfiI CCNNNNNNNGG 3 cut(s) 63, 577, 648
AflII CTTAAG 1 cut(s) 164
AflIII ACRYGT 1 cut(s) 660
AgsI TTSAA 6 cut(s) 136, 158, 412, 467, 806, 875
AjnI CCWGG 2 cut(s) 716, 754
Alw26I GTCTC 2 cut(s) 386, 502
AlwI GGATC 2 cut(s) 297, 310
AlwNI CAGNNNCTG 1 cut(s) 152
AoxI GGCC 4 cut(s) 168, 650, 714, 862
ApeKI GCWGC 4 cut(s) 230, 479, 686, 812
ApoI RAATTY 1 cut(s) 605
Asp700I GAANNNNTTC 1 cut(s) 450
AspS9I GGNCC 1 cut(s) 821
AsuC2I CCSGG 1 cut(s) 790
AsuHPI GGTGA 2 cut(s) 307, 565
AvaII GGWCC 1 cut(s) 821
BalI TGGCCA 1 cut(s) 716
BamHI GGATCC 1 cut(s) 302
BbsI GAAGAC 1 cut(s) 657
BbvI GCAGC 4 cut(s) 217, 491, 698, 799
BccI CCATC 1 cut(s) 23
BceAI ACGGC 1 cut(s) 411
BciT130I CCWGG 2 cut(s) 718, 756
BciVI GTATCC 1 cut(s) 735
BcnI CCSGG 1 cut(s) 790
BcoDI GTCTC 2 cut(s) 386, 502
BfaI CTAG 2 cut(s) 203, 554
BfmI CTRYAG 1 cut(s) 813
BfrI CTTAAG 1 cut(s) 164
BfuI GTATCC 1 cut(s) 735
BisI GCNGC 5 cut(s) 231, 480, 653, 687, 813
BlsI GCNGC 5 cut(s) 232, 481, 654, 688, 814
Bme1390I CCNGG 3 cut(s) 718, 756, 790
Bme18I GGWCC 1 cut(s) 821
BmgT120I GGNCC 1 cut(s) 821
BmiI GGNNCC 3 cut(s) 55, 304, 336
BmrFI CCNGG 3 cut(s) 718, 756, 790
BmrI ACTGGG 2 cut(s) 250, 518
BmuI ACTGGG 2 cut(s) 250, 518
BpiI GAAGAC 1 cut(s) 657
BpmI CTGGAG 2 cut(s) 72, 632
Bpu10I CCTNAGC 1 cut(s) 518
BpuEI CTTGAG 2 cut(s) 95, 324
BpuMI CCSGG 1 cut(s) 790
BsaJI CCNNGG 2 cut(s) 298, 576
BsaXI ACNNNNNCTCC 2 cut(s) 96, 126
Bsc4I CCNNNNNNNGG 3 cut(s) 63, 577, 648
Bse1I ACTGG 3 cut(s) 55, 256, 513
Bse3DI GCAATG 1 cut(s) 225
BseBI CCWGG 2 cut(s) 718, 756
BseDI CCNNGG 2 cut(s) 298, 576
BseGI GGATG 2 cut(s) 622, 890
BseLI CCNNNNNNNGG 3 cut(s) 63, 577, 648
BseMI GCAATG 1 cut(s) 225
BseNI ACTGG 3 cut(s) 55, 256, 513
BseXI GCAGC 4 cut(s) 217, 491, 698, 799
BshFI GGCC 4 cut(s) 170, 652, 716, 864
BsiSI CCGG 1 cut(s) 789
BslFI GGGAC 1 cut(s) 525
BslI CCNNNNNNNGG 3 cut(s) 63, 577, 648
BsmAI GTCTC 2 cut(s) 386, 502
BsmBI CGTCTC 1 cut(s) 386
BsmFI GGGAC 1 cut(s) 525
BsmI GAATGC 1 cut(s) 700
BsnI GGCC 4 cut(s) 170, 652, 716, 864
Bsp143I GATC 1 cut(s) 302
BspACI CCGC 2 cut(s) 458, 653
BspANI GGCC 4 cut(s) 170, 652, 716, 864
BspLI GGNNCC 3 cut(s) 55, 304, 336
BspMAI CTGCAG 1 cut(s) 817
BspPI GGATC 2 cut(s) 297, 310
BspTI CTTAAG 1 cut(s) 164
BsrDI GCAATG 1 cut(s) 225
BsrI ACTGG 3 cut(s) 55, 256, 513
BssECI CCNNGG 2 cut(s) 298, 576
BssMI GATC 1 cut(s) 302
BssT1I CCWWGG 1 cut(s) 298
Bst2UI CCWGG 2 cut(s) 718, 756
Bst4CI ACNGT 1 cut(s) 802
Bst6I CTCTTC 3 cut(s) 131, 505, 879
BstAFI CTTAAG 1 cut(s) 164
BstC8I GCNNGC 2 cut(s) 650, 834
BstDEI CTNAG 2 cut(s) 349, 518
BstENI CCTNNNNNAGG 1 cut(s) 575
BstF5I GGATG 2 cut(s) 622, 890
BstKTI GATC 1 cut(s) 305
BstMAI GTCTC 2 cut(s) 386, 502
BstMBI GATC 1 cut(s) 302
BstMWI GCNNNNNNNGC 4 cut(s) 236, 695, 743, 842
BstNI CCWGG 2 cut(s) 718, 756
BstNSI RCATGY 1 cut(s) 664
BstSCI CCNGG 3 cut(s) 716, 754, 788
BstSFI CTRYAG 1 cut(s) 813
BstV1I GCAGC 4 cut(s) 217, 491, 698, 799
BstV2I GAAGAC 1 cut(s) 657
BstX2I RGATCY 1 cut(s) 302
BstYI RGATCY 1 cut(s) 302
BsuI GTATCC 1 cut(s) 735
BsuRI GGCC 4 cut(s) 170, 652, 716, 864
BtsCI GGATG 2 cut(s) 622, 890
BtsIMutI CAGTG 2 cut(s) 362, 506
Cac8I GCNNGC 2 cut(s) 650, 834
CaiI CAGNNNCTG 1 cut(s) 152
Cfr13I GGNCC 1 cut(s) 821
Csp6I GTAC 1 cut(s) 116
CviAII CATG 1 cut(s) 661
CviQI GTAC 1 cut(s) 116
DdeI CTNAG 2 cut(s) 349, 518
DpnI GATC 1 cut(s) 304
DpnII GATC 1 cut(s) 302
DrdI GACNNNNNNGTC 1 cut(s) 886
DseDI GACNNNNNNGTC 1 cut(s) 886
EaeI YGGCCR 2 cut(s) 650, 714
Eam1104I CTCTTC 3 cut(s) 131, 505, 879
EarI CTCTTC 3 cut(s) 131, 505, 879
Eco130I CCWWGG 1 cut(s) 298
Eco147I AGGCCT 1 cut(s) 170
Eco32I GATATC 1 cut(s) 346
Eco47I GGWCC 1 cut(s) 821
Eco57I CTGAAG 1 cut(s) 486
EcoNI CCTNNNNNAGG 1 cut(s) 575
EcoO109I RGGNCCY 1 cut(s) 821
EcoRII CCWGG 2 cut(s) 716, 754
EcoRV GATATC 1 cut(s) 346
EcoT14I CCWWGG 1 cut(s) 298
ErhI CCWWGG 1 cut(s) 298
Esp3I CGTCTC 1 cut(s) 386
FaeI CATG 1 cut(s) 664
FaiI YATR 6 cut(s) 310, 388, 390, 426, 662, 702
FalI AAGNNNNNCTT 2 cut(s) 438, 470
FaqI GGGAC 1 cut(s) 525
FatI CATG 1 cut(s) 660
FauNDI CATATG 1 cut(s) 388
Fnu4HI GCNGC 5 cut(s) 231, 480, 653, 687, 813
FokI GGATG 2 cut(s) 629, 877
Fsp4HI GCNGC 5 cut(s) 231, 480, 653, 687, 813
FspBI CTAG 2 cut(s) 203, 554
GluI GCNGC 5 cut(s) 231, 480, 653, 687, 813
GsuI CTGGAG 2 cut(s) 72, 632
HaeIII GGCC 4 cut(s) 170, 652, 716, 864
HapII CCGG 1 cut(s) 789
Hin1II CATG 1 cut(s) 664
HindIII AAGCTT 2 cut(s) 161, 266
HinfI GANTC 7 cut(s) 26, 294, 429, 638, 708, 848, 887
HpaII CCGG 1 cut(s) 789
HphI GGTGA 2 cut(s) 307, 565
Hpy166II GTNNAC 1 cut(s) 589
Hpy188I TCNGA 3 cut(s) 184, 475, 693
Hpy188III TCNNGA 2 cut(s) 341, 611
Hpy8I GTNNAC 1 cut(s) 589
Hpy99I CGWCG 1 cut(s) 329
HpyAV CCTTC 2 cut(s) 541, 812
HpyCH4III ACNGT 1 cut(s) 802
HpyCH4IV ACGT 1 cut(s) 324
HpyCH4V TGCA 4 cut(s) 290, 382, 530, 815
HpyF10VI GCNNNNNNNGC 4 cut(s) 236, 695, 743, 842
HpyF3I CTNAG 2 cut(s) 349, 518
HpySE526I ACGT 1 cut(s) 324
Hsp92II CATG 1 cut(s) 664
Kzo9I GATC 1 cut(s) 302
LmnI GCTCC 3 cut(s) 53, 334, 550
Lsp1109I GCAGC 4 cut(s) 217, 491, 698, 799
MaeI CTAG 2 cut(s) 203, 554
MaeII ACGT 1 cut(s) 324
MaeIII GTNAC 1 cut(s) 46
MalI GATC 1 cut(s) 304
MboI GATC 1 cut(s) 302
MboII GAAGA 7 cut(s) 148, 446, 455, 492, 657, 818, 896
MflI RGATCY 1 cut(s) 302
MlsI TGGCCA 1 cut(s) 716
MluCI AATT 3 cut(s) 89, 605, 732
MluNI TGGCCA 1 cut(s) 716
MlyI GAGTC 5 cut(s) 35, 303, 717, 842, 896
Mox20I TGGCCA 1 cut(s) 716
MroXI GAANNNNTTC 1 cut(s) 450
MscI TGGCCA 1 cut(s) 716
MseI TTAA 4 cut(s) 165, 282, 795, 839
Msp20I TGGCCA 1 cut(s) 716
MspCI CTTAAG 1 cut(s) 164
MspI CCGG 1 cut(s) 789
MspR9I CCNGG 3 cut(s) 718, 756, 790
Mva1269I GAATGC 1 cut(s) 700
MvaI CCWGG 2 cut(s) 718, 756
MwoI GCNNNNNNNGC 4 cut(s) 236, 695, 743, 842
NciI CCSGG 1 cut(s) 790
NdeI CATATG 1 cut(s) 388
NdeII GATC 1 cut(s) 302
NlaIII CATG 1 cut(s) 664
NlaIV GGNNCC 3 cut(s) 55, 304, 336
NspI RCATGY 1 cut(s) 664
PceI AGGCCT 1 cut(s) 170
PciI ACATGT 1 cut(s) 660
PctI GAATGC 1 cut(s) 700
PdmI GAANNNNTTC 1 cut(s) 450
PfeI GAWTC 2 cut(s) 429, 638
PflMI CCANNNNNTGG 1 cut(s) 648
PkrI GCNGC 5 cut(s) 232, 481, 654, 688, 814
PleI GAGTC 5 cut(s) 34, 302, 716, 842, 895
PpsI GAGTC 5 cut(s) 34, 302, 716, 842, 895
PpuMI RGGWCCY 1 cut(s) 821
PscI ACATGT 1 cut(s) 660
Psp5II RGGWCCY 1 cut(s) 821
Psp6I CCWGG 2 cut(s) 716, 754
PspGI CCWGG 2 cut(s) 716, 754
PspN4I GGNNCC 3 cut(s) 55, 304, 336
PspPI GGNCC 1 cut(s) 821
PspPPI RGGWCCY 1 cut(s) 821
PstI CTGCAG 1 cut(s) 817
PstNI CAGNNNCTG 1 cut(s) 152
PsuI RGATCY 1 cut(s) 302
RsaI GTAC 1 cut(s) 117
RsaNI GTAC 1 cut(s) 116
SaqAI TTAA 4 cut(s) 165, 282, 795, 839
SatI GCNGC 5 cut(s) 231, 480, 653, 687, 813
Sau3AI GATC 1 cut(s) 302
Sau96I GGNCC 1 cut(s) 821
SchI GAGTC 5 cut(s) 35, 303, 717, 842, 896
ScrFI CCNGG 3 cut(s) 718, 756, 790
SfcI CTRYAG 1 cut(s) 813
SinI GGWCC 1 cut(s) 821
SmlI CTYRAG 3 cut(s) 74, 164, 339
SmoI CTYRAG 3 cut(s) 74, 164, 339
Sse9I AATT 3 cut(s) 89, 605, 732
SseBI AGGCCT 1 cut(s) 170
SsiI CCGC 2 cut(s) 458, 653
SspMI CTAG 2 cut(s) 203, 554
StuI AGGCCT 1 cut(s) 170
StyD4I CCNGG 3 cut(s) 716, 754, 788
StyI CCWWGG 1 cut(s) 298
TaaI ACNGT 1 cut(s) 802
TaiI ACGT 1 cut(s) 327
TasI AATT 3 cut(s) 89, 605, 732
TauI GCSGC 1 cut(s) 655
TfiI GAWTC 2 cut(s) 429, 638
Tru1I TTAA 4 cut(s) 165, 282, 795, 839
Tru9I TTAA 4 cut(s) 165, 282, 795, 839
TscAI CASTG 2 cut(s) 369, 513
TseI GCWGC 4 cut(s) 230, 479, 686, 812
TspDTI ATGAA 1 cut(s) 185
TspGWI ACGGA 1 cut(s) 896
TspRI CASTG 2 cut(s) 369, 513
Van91I CCANNNNNTGG 1 cut(s) 648
Vha464I CTTAAG 1 cut(s) 164
VpaK11BI GGWCC 1 cut(s) 821
XagI CCTNNNNNAGG 1 cut(s) 575
XapI RAATTY 1 cut(s) 605
XceI RCATGY 1 cut(s) 664
XcmI CCANNNNNNNNNTGG 1 cut(s) 232
XmnI GAANNNNTTC 1 cut(s) 450
XspI CTAG 2 cut(s) 203, 554
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.