RLG00000003860

Salutaridine reductase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0001077
Physical Location & Seq
Forward (+)
53625142 .. 53626896
1755 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000003860

Sequence Viewer

Length: 882 bp
ATGGCGGAAACTACCATCAATTTTGGGTCAAAGAAGATTGCAGTTGTTACTGGAGCCAACAAAGGGATTGGACTTGAGATTAGTAAGCAATTAGCTTCTAAAGGAGTTGGGGTGGTATTAACAGCAAGAGATGCGAAGAGAGGAACAGAAGCTGCTGAAAGCCTAAAGGCTTCTGGTTTCACCGACGTGGTCTTCCATCAGCTAGATGTAACTGCCCCAACTACCATTGCTTCTTTGGCAAACTTTCTCAAAACGCAATTCAGAAAGCTTGACATATTGGTTAACAATGCAGGAGTCCTTGGATCTGTATTCCTCGCAGACGACATAGTTGGGCCTGACAATGTTAAACAAAAAACAGTGCAAACTTACGAGACAGCGGTGGATTGTTTGAAAACAAACTATTATGGAATCAAGCAACTCACAGAAGCACTAGTTCCACTACTTCAAAAATCTGAAGCTCCAAAGATAGTCAATGTCTCTTCCACACTGGGACAGCTAAGACTTATTTCAAATGAGAAAGCAAAGAAGGAGCTGGGAGATGCGGATAACCTCACCGAGGAGAAAGTGGACAAGCTAGTTGAGGAATTTCTAGAGGATGTGAAGCATGATTTGATAGAATCCAAAGGATGGCCTTTAGATATATCTGCCTACATTGTATCAAAAGCAGCTCTTAATGCTTATACAAGAGTCGTGGCAAAGAAGTATCCCCACATTGCTATAAACGCAGTTAGTCCAGGTTATACCAAAACAGATCTCAACAATAATACCGGGATTCTCACAGTTGAAGAAGCTGCACTTGGTCCTGTGAAGCTGGCTTTGATAGTCGAAACTGGAATTTCAGGGCTCTTCTTTGACCGGATTGAAGAGTCAACCTTTGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00902 ko01110 map00902 map01110
Pfam Domains
Protein Families
adh_short adh_short_C2

Protein Analysis

294

Amino Acids

31.57

Weight (kDa)

5.59

Isoelectric Point (pI)

25.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
adh_short PF00106 12 - 168 2.1e-28 short chain dehydrogenase
KR PF08659 15 - 106 9.8e-08 KR domain
adh_short_C2 PF13561 18 - 165 2.5e-18 Enoyl-(Acyl carrier protein) reductase
adh_short_C2 PF13561 215 - 255 2.2e-06 Enoyl-(Acyl carrier protein) reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 627
AciI CCGC 3 cut(s) 5, 377, 542
AclWI GGATC 1 cut(s) 310
AcsI RAATTY 2 cut(s) 584, 834
AcuI CTGAAG 1 cut(s) 474
AfiI CCNNNNNNNGG 3 cut(s) 63, 556, 627
AgsI TTSAA 5 cut(s) 391, 446, 510, 785, 863
AhlI ACTAGT 1 cut(s) 430
AjiI CACGTC 1 cut(s) 187
AjnI CCWGG 1 cut(s) 733
AjuI GAANNNNNNNTTGG 2 cut(s) 780, 812
AleI CACNNNNGTG 1 cut(s) 185
Alw26I GTCTC 2 cut(s) 365, 481
AlwI GGATC 1 cut(s) 310
AlwNI CAGNNNCTG 1 cut(s) 152
AoxI GGCC 2 cut(s) 332, 629
ApeKI GCWGC 3 cut(s) 152, 665, 791
ApoI RAATTY 2 cut(s) 584, 834
AspS9I GGNCC 2 cut(s) 332, 800
AsuC2I CCSGG 1 cut(s) 769
AsuHPI GGTGA 2 cut(s) 172, 544
AvaII GGWCC 1 cut(s) 800
BanII GRGCYC 1 cut(s) 846
BbsI GAAGAC 1 cut(s) 184
BbvI GCAGC 3 cut(s) 139, 677, 778
BccI CCATC 3 cut(s) 23, 204, 621
BciT130I CCWGG 1 cut(s) 735
BciVI GTATCC 1 cut(s) 714
BcnI CCSGG 1 cut(s) 769
BcoDI GTCTC 2 cut(s) 365, 481
BcuI ACTAGT 1 cut(s) 430
BfaI CTAG 4 cut(s) 203, 431, 575, 590
BfuI GTATCC 1 cut(s) 714
BglII AGATCT 1 cut(s) 751
BisI GCNGC 3 cut(s) 153, 666, 792
BlsI GCNGC 3 cut(s) 154, 667, 793
Bme1390I CCNGG 2 cut(s) 735, 769
Bme18I GGWCC 1 cut(s) 800
BmgBI CACGTC 1 cut(s) 187
BmgT120I GGNCC 2 cut(s) 332, 800
BmiI GGNNCC 1 cut(s) 55
BmrFI CCNGG 2 cut(s) 735, 769
BmrI ACTGGG 1 cut(s) 497
BmsI GCATC 2 cut(s) 121, 529
BmuI ACTGGG 1 cut(s) 497
BpiI GAAGAC 1 cut(s) 184
BpmI CTGGAG 1 cut(s) 72
BpuEI CTTGAG 1 cut(s) 95
BpuMI CCSGG 1 cut(s) 769
BsaJI CCNNGG 2 cut(s) 298, 555
BsaWI WCCGGW 1 cut(s) 855
BsaXI ACNNNNNCTCC 2 cut(s) 96, 126
Bsc4I CCNNNNNNNGG 3 cut(s) 63, 556, 627
Bse1I ACTGG 3 cut(s) 55, 492, 835
Bse3DI GCAATG 2 cut(s) 225, 711
BseBI CCWGG 1 cut(s) 735
BseDI CCNNGG 2 cut(s) 298, 555
BseGI GGATG 2 cut(s) 601, 632
BseLI CCNNNNNNNGG 3 cut(s) 63, 556, 627
BseMI GCAATG 2 cut(s) 225, 711
BseNI ACTGG 3 cut(s) 55, 492, 835
BseRI GAGGAG 1 cut(s) 572
BseXI GCAGC 3 cut(s) 139, 677, 778
BseYI CCCAGC 1 cut(s) 532
BsgI GTGCAG 1 cut(s) 777
BshFI GGCC 2 cut(s) 334, 631
BsiSI CCGG 2 cut(s) 768, 856
BslFI GGGAC 1 cut(s) 504
BslI CCNNNNNNNGG 3 cut(s) 63, 556, 627
BsmAI GTCTC 2 cut(s) 365, 481
BsmFI GGGAC 1 cut(s) 504
BsnI GGCC 2 cut(s) 334, 631
Bsp1286I GDGCHC 1 cut(s) 846
Bsp143I GATC 2 cut(s) 302, 751
BspACI CCGC 3 cut(s) 5, 377, 542
BspANI GGCC 2 cut(s) 334, 631
BspLI GGNNCC 1 cut(s) 55
BspPI GGATC 1 cut(s) 310
BspQI GCTCTTC 1 cut(s) 851
BsrDI GCAATG 2 cut(s) 225, 711
BsrI ACTGG 3 cut(s) 55, 492, 835
BssECI CCNNGG 2 cut(s) 298, 555
BssMI GATC 2 cut(s) 302, 751
BssT1I CCWWGG 1 cut(s) 298
Bst2UI CCWGG 1 cut(s) 735
Bst4CI ACNGT 2 cut(s) 358, 781
Bst6I CTCTTC 4 cut(s) 131, 484, 851, 858
BstAPI GCANNNNNTGC 1 cut(s) 131
BstC8I GCNNGC 1 cut(s) 813
BstDEI CTNAG 1 cut(s) 497
BstENI CCTNNNNNAGG 1 cut(s) 554
BstF5I GGATG 2 cut(s) 601, 632
BstKTI GATC 2 cut(s) 305, 754
BstMAI GTCTC 2 cut(s) 365, 481
BstMBI GATC 2 cut(s) 302, 751
BstMWI GCNNNNNNNGC 4 cut(s) 131, 236, 674, 722
BstNI CCWGG 1 cut(s) 735
BstSCI CCNGG 2 cut(s) 733, 767
BstV1I GCAGC 3 cut(s) 139, 677, 778
BstV2I GAAGAC 1 cut(s) 184
BstX2I RGATCY 2 cut(s) 302, 751
BstYI RGATCY 2 cut(s) 302, 751
BsuI GTATCC 1 cut(s) 714
BsuRI GGCC 2 cut(s) 334, 631
BtrI CACGTC 1 cut(s) 187
BtsCI GGATG 2 cut(s) 601, 632
BtsIMutI CAGTG 2 cut(s) 363, 485
Cac8I GCNNGC 1 cut(s) 813
CaiI CAGNNNCTG 1 cut(s) 152
Cfr13I GGNCC 2 cut(s) 332, 800
CspCI CAANNNNNGTGG 2 cut(s) 672, 707
CviAII CATG 1 cut(s) 605
DdeI CTNAG 1 cut(s) 497
DpnI GATC 2 cut(s) 304, 753
DpnII GATC 2 cut(s) 302, 751
Eam1104I CTCTTC 4 cut(s) 131, 484, 851, 858
EarI CTCTTC 4 cut(s) 131, 484, 851, 858
EciI GGCGGA 1 cut(s) 20
Eco130I CCWWGG 1 cut(s) 298
Eco24I GRGCYC 1 cut(s) 846
Eco47I GGWCC 1 cut(s) 800
Eco57I CTGAAG 1 cut(s) 474
EcoNI CCTNNNNNAGG 1 cut(s) 554
EcoRII CCWGG 1 cut(s) 733
EcoT14I CCWWGG 1 cut(s) 298
EcoT38I GRGCYC 1 cut(s) 846
ErhI CCWWGG 1 cut(s) 298
FaeI CATG 1 cut(s) 608
FaiI YATR 8 cut(s) 275, 326, 405, 606, 641, 681, 719, 741
FalI AAGNNNNNCTT 4 cut(s) 654, 686, 780, 812
FaqI GGGAC 1 cut(s) 504
FatI CATG 1 cut(s) 604
Fnu4HI GCNGC 3 cut(s) 153, 666, 792
FokI GGATG 2 cut(s) 608, 639
FriOI GRGCYC 1 cut(s) 846
Fsp4HI GCNGC 3 cut(s) 153, 666, 792
FspBI CTAG 4 cut(s) 203, 431, 575, 590
GluI GCNGC 3 cut(s) 153, 666, 792
GsaI CCCAGC 1 cut(s) 536
GsuI CTGGAG 1 cut(s) 72
HaeIII GGCC 2 cut(s) 334, 631
HapII CCGG 2 cut(s) 768, 856
Hin1II CATG 1 cut(s) 608
HincII GTYRAC 2 cut(s) 283, 870
HindII GTYRAC 2 cut(s) 283, 870
HindIII AAGCTT 1 cut(s) 266
HinfI GANTC 6 cut(s) 294, 408, 617, 687, 772, 866
HpaI GTTAAC 1 cut(s) 283
HpaII CCGG 2 cut(s) 768, 856
HphI GGTGA 2 cut(s) 172, 544
Hpy166II GTNNAC 3 cut(s) 283, 568, 870
Hpy188I TCNGA 2 cut(s) 263, 454
Hpy188III TCNNGA 1 cut(s) 590
Hpy8I GTNNAC 3 cut(s) 283, 568, 870
Hpy99I CGWCG 1 cut(s) 188
HpyAV CCTTC 1 cut(s) 520
HpyCH4III ACNGT 2 cut(s) 358, 781
HpyCH4IV ACGT 1 cut(s) 186
HpyCH4V TGCA 4 cut(s) 41, 290, 361, 794
HpyF10VI GCNNNNNNNGC 4 cut(s) 131, 236, 674, 722
HpyF3I CTNAG 1 cut(s) 497
HpySE526I ACGT 1 cut(s) 186
Hsp92II CATG 1 cut(s) 608
KspAI GTTAAC 1 cut(s) 283
Kzo9I GATC 2 cut(s) 302, 751
LguI GCTCTTC 1 cut(s) 851
LmnI GCTCC 3 cut(s) 53, 463, 529
Lsp1109I GCAGC 3 cut(s) 139, 677, 778
LweI GCATC 2 cut(s) 121, 529
MaeI CTAG 4 cut(s) 203, 431, 575, 590
MaeII ACGT 1 cut(s) 186
MaeIII GTNAC 2 cut(s) 46, 208
MalI GATC 2 cut(s) 304, 753
MboI GATC 2 cut(s) 302, 751
MboII GAAGA 7 cut(s) 46, 148, 184, 471, 797, 838, 875
MflI RGATCY 2 cut(s) 302, 751
MhlI GDGCHC 1 cut(s) 846
MluCI AATT 5 cut(s) 19, 89, 257, 584, 834
MlyI GAGTC 3 cut(s) 303, 696, 875
MnlI CCTC 6 cut(s) 134, 323, 550, 560, 574, 586
MseI TTAA 4 cut(s) 119, 282, 345, 672
MslI CAYNNNNRTG 1 cut(s) 185
MspA1I CMGCKG 1 cut(s) 377
MspI CCGG 2 cut(s) 768, 856
MspR9I CCNGG 2 cut(s) 735, 769
MvaI CCWGG 1 cut(s) 735
MwoI GCNNNNNNNGC 4 cut(s) 131, 236, 674, 722
NciI CCSGG 1 cut(s) 769
NdeII GATC 2 cut(s) 302, 751
NlaIII CATG 1 cut(s) 608
NlaIV GGNNCC 1 cut(s) 55
OliI CACNNNNGTG 1 cut(s) 185
PciSI GCTCTTC 1 cut(s) 851
PfeI GAWTC 3 cut(s) 408, 617, 772
PflFI GACNNNGTC 1 cut(s) 188
PflMI CCANNNNNTGG 1 cut(s) 627
PkrI GCNGC 3 cut(s) 154, 667, 793
PleI GAGTC 3 cut(s) 302, 695, 874
PpsI GAGTC 3 cut(s) 302, 695, 874
Psp6I CCWGG 1 cut(s) 733
PspFI CCCAGC 1 cut(s) 532
PspGI CCWGG 1 cut(s) 733
PspN4I GGNNCC 1 cut(s) 55
PspPI GGNCC 2 cut(s) 332, 800
PstNI CAGNNNCTG 1 cut(s) 152
PsuI RGATCY 2 cut(s) 302, 751
PsyI GACNNNGTC 1 cut(s) 188
RseI CAYNNNNRTG 1 cut(s) 185
SapI GCTCTTC 1 cut(s) 851
SaqAI TTAA 4 cut(s) 119, 282, 345, 672
SatI GCNGC 3 cut(s) 153, 666, 792
Sau3AI GATC 2 cut(s) 302, 751
Sau96I GGNCC 2 cut(s) 332, 800
SchI GAGTC 3 cut(s) 303, 696, 875
ScrFI CCNGG 2 cut(s) 735, 769
SduI GDGCHC 1 cut(s) 846
SfaNI GCATC 2 cut(s) 121, 529
SinI GGWCC 1 cut(s) 800
SmiMI CAYNNNNRTG 1 cut(s) 185
SmlI CTYRAG 1 cut(s) 74
SmoI CTYRAG 1 cut(s) 74
SpeI ACTAGT 1 cut(s) 430
Sse9I AATT 5 cut(s) 19, 89, 257, 584, 834
SsiI CCGC 3 cut(s) 5, 377, 542
SspMI CTAG 4 cut(s) 203, 431, 575, 590
StyD4I CCNGG 2 cut(s) 733, 767
StyI CCWWGG 1 cut(s) 298
TaaI ACNGT 2 cut(s) 358, 781
TaiI ACGT 1 cut(s) 189
TaqI TCGA 1 cut(s) 825
TasI AATT 5 cut(s) 19, 89, 257, 584, 834
TfiI GAWTC 3 cut(s) 408, 617, 772
Tru1I TTAA 4 cut(s) 119, 282, 345, 672
Tru9I TTAA 4 cut(s) 119, 282, 345, 672
TscAI CASTG 2 cut(s) 363, 492
TseI GCWGC 3 cut(s) 152, 665, 791
TspRI CASTG 2 cut(s) 363, 492
Tth111I GACNNNGTC 1 cut(s) 188
Van91I CCANNNNNTGG 1 cut(s) 627
VpaK11BI GGWCC 1 cut(s) 800
XagI CCTNNNNNAGG 1 cut(s) 554
XapI RAATTY 2 cut(s) 584, 834
XbaI TCTAGA 1 cut(s) 589
XcmI CCANNNNNNNNNTGG 1 cut(s) 232
XspI CTAG 4 cut(s) 203, 431, 575, 590
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.