Rmu_sc0004229.1_g000022

Salutaridine reductase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004229.1
OG0001077
Physical Location & Seq
Forward (+)
90693 .. 94586
3894 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0004229.1_g000022.1.cds

Sequence Viewer

Length: 858 bp
atgcatgtgagagaggagagagagagagctgaccgggtcggacagagcgagctgggattgggccgagctgtgagggaagctccgcggagagaagaaggaagaaaggaagttgctggcaagtgcagaaacgacgtcatttcaatggaggttgcgcggtgctggagtggtgggattgcagttgttactggagccaacaaagggattggacttgagattagtaagcaattagcttctaaaggagttggggtggtattaacagcaagagatgcgaagagaggaacagaagcagctgaaagcctaaaggcttctggtttctccgatgtggtcttccatcagctagatgtaacagccccaactagcattgcttctttggcaaactttctcaaaacgcaattcagaaagcttgacatattggttaacaacgcaggagtccttggatctgtattcctcgcagacgacatagttgggcctgacaatcttatttcaaatgagaaagcaaagaaggagctaggagatgcggataacctcaccgaggagaaagtggacaagctagttgaggaatttctagaggatgtgaagcatgattcgatagaatccaaaggatggcctttagatatatctgcctacattgtatcgaaagcagctcttaatgcttatacaagagtcgtggcaaagaagtatccccacattgctattaacgcagttagtccaggttataccaaaacagatctcaacaataatactgggattctcacagttgaagaagctgcacttggtcctgtgaagctggcattgacagccgaaactggaatttcaggcctcttctttgaccggactgaagagtcaacctttgattaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00902 ko01110 map00902 map01110
Pfam Domains
Protein Families
adh_short adh_short_C2

Protein Analysis

285

Amino Acids

30.71

Weight (kDa)

5.47

Isoelectric Point (pI)

30.55

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 841
AatII GACGTC 1 cut(s) 135
AccB7I CCANNNNNTGG 1 cut(s) 603
AccII CGCG 2 cut(s) 85, 154
AciI CCGC 4 cut(s) 83, 85, 154, 518
AclWI GGATC 1 cut(s) 445
AcsI RAATTY 2 cut(s) 560, 810
AcuI CTGAAG 1 cut(s) 858
AcyI GRCGYC 1 cut(s) 132
AfiI CCNNNNNNNGG 3 cut(s) 198, 532, 603
AgsI TTSAA 3 cut(s) 141, 486, 761
AjnI CCWGG 1 cut(s) 709
AjuI GAANNNNNNNTTGG 2 cut(s) 756, 788
AlwI GGATC 1 cut(s) 445
AoxI GGCC 4 cut(s) 61, 467, 605, 817
ApeKI GCWGC 3 cut(s) 287, 641, 767
ApoI RAATTY 2 cut(s) 560, 810
AspLEI GCGC 1 cut(s) 154
AspS9I GGNCC 3 cut(s) 61, 467, 776
AsuC2I CCSGG 1 cut(s) 35
AsuHPI GGTGA 1 cut(s) 520
AvaII GGWCC 1 cut(s) 776
BbsI GAAGAC 1 cut(s) 319
BbvI GCAGC 3 cut(s) 299, 653, 754
BccI CCATC 2 cut(s) 339, 597
BciT130I CCWGG 1 cut(s) 711
BciVI GTATCC 1 cut(s) 690
BcnI CCSGG 1 cut(s) 35
BfaI CTAG 5 cut(s) 338, 357, 509, 551, 566
BfuI GTATCC 1 cut(s) 690
BglII AGATCT 1 cut(s) 727
BisI GCNGC 3 cut(s) 288, 642, 768
BlsI GCNGC 3 cut(s) 289, 643, 769
Bme1390I CCNGG 2 cut(s) 35, 711
Bme18I GGWCC 1 cut(s) 776
BmgT120I GGNCC 3 cut(s) 61, 467, 776
BmiI GGNNCC 1 cut(s) 190
BmrFI CCNGG 2 cut(s) 35, 711
BmrI ACTGGG 1 cut(s) 753
BmsI GCATC 2 cut(s) 256, 505
BmuI ACTGGG 1 cut(s) 753
BpiI GAAGAC 1 cut(s) 319
BplI GAGNNNNNCTC 2 cut(s) 64, 96
BpmI CTGGAG 2 cut(s) 181, 207
BpuEI CTTGAG 1 cut(s) 230
BpuMI CCSGG 1 cut(s) 35
BsaHI GRCGYC 1 cut(s) 132
BsaJI CCNNGG 3 cut(s) 83, 433, 531
BsaWI WCCGGW 1 cut(s) 831
BsaXI ACNNNNNCTCC 2 cut(s) 231, 261
Bsc4I CCNNNNNNNGG 3 cut(s) 198, 532, 603
Bse1I ACTGG 3 cut(s) 190, 748, 811
Bse3DI GCAATG 2 cut(s) 360, 687
BseBI CCWGG 1 cut(s) 711
BseDI CCNNGG 3 cut(s) 83, 433, 531
BseGI GGATG 2 cut(s) 577, 608
BseLI CCNNNNNNNGG 3 cut(s) 198, 532, 603
BseMI GCAATG 2 cut(s) 360, 687
BseNI ACTGG 3 cut(s) 190, 748, 811
BseRI GAGGAG 2 cut(s) 29, 548
BseXI GCAGC 3 cut(s) 299, 653, 754
BseYI CCCAGC 1 cut(s) 52
BsgI GTGCAG 2 cut(s) 142, 753
Bsh1236I CGCG 2 cut(s) 85, 154
BshFI GGCC 4 cut(s) 63, 469, 607, 819
BsiSI CCGG 2 cut(s) 34, 832
BslI CCNNNNNNNGG 3 cut(s) 198, 532, 603
BsnI GGCC 4 cut(s) 63, 469, 607, 819
Bsp143I GATC 2 cut(s) 437, 727
BspACI CCGC 4 cut(s) 83, 85, 154, 518
BspANI GGCC 4 cut(s) 63, 469, 607, 819
BspFNI CGCG 2 cut(s) 85, 154
BspLI GGNNCC 1 cut(s) 190
BspPI GGATC 1 cut(s) 445
BsrDI GCAATG 2 cut(s) 360, 687
BsrI ACTGG 3 cut(s) 190, 748, 811
BssECI CCNNGG 3 cut(s) 83, 433, 531
BssMI GATC 2 cut(s) 437, 727
BssNI GRCGYC 1 cut(s) 132
BssT1I CCWWGG 1 cut(s) 433
Bst2UI CCWGG 1 cut(s) 711
Bst4CI ACNGT 1 cut(s) 757
Bst6I CTCTTC 3 cut(s) 266, 827, 834
BstACI GRCGYC 1 cut(s) 132
BstAPI GCANNNNNTGC 1 cut(s) 266
BstC8I GCNNGC 3 cut(s) 50, 115, 789
BstDSI CCRYGG 1 cut(s) 83
BstENI CCTNNNNNAGG 1 cut(s) 530
BstF5I GGATG 2 cut(s) 577, 608
BstFNI CGCG 2 cut(s) 85, 154
BstHHI GCGC 1 cut(s) 154
BstKTI GATC 2 cut(s) 440, 730
BstMBI GATC 2 cut(s) 437, 727
BstMWI GCNNNNNNNGC 5 cut(s) 266, 371, 650, 698, 797
BstNI CCWGG 1 cut(s) 711
BstNSI RCATGY 1 cut(s) 8
BstSCI CCNGG 2 cut(s) 33, 709
BstUI CGCG 2 cut(s) 85, 154
BstV1I GCAGC 3 cut(s) 299, 653, 754
BstV2I GAAGAC 1 cut(s) 319
BstX2I RGATCY 2 cut(s) 437, 727
BstYI RGATCY 2 cut(s) 437, 727
BsuI GTATCC 1 cut(s) 690
BsuRI GGCC 4 cut(s) 63, 469, 607, 819
BtgI CCRYGG 1 cut(s) 83
BtsCI GGATG 2 cut(s) 577, 608
Cac8I GCNNGC 3 cut(s) 50, 115, 789
CfoI GCGC 1 cut(s) 154
Cfr13I GGNCC 3 cut(s) 61, 467, 776
Cfr42I CCGCGG 1 cut(s) 86
CspCI CAANNNNNGTGG 2 cut(s) 648, 683
CviAII CATG 2 cut(s) 5, 581
DpnI GATC 2 cut(s) 439, 729
DpnII GATC 2 cut(s) 437, 727
DrdI GACNNNNNNGTC 1 cut(s) 841
DseDI GACNNNNNNGTC 1 cut(s) 841
Eam1104I CTCTTC 3 cut(s) 266, 827, 834
EarI CTCTTC 3 cut(s) 266, 827, 834
Eco130I CCWWGG 1 cut(s) 433
Eco147I AGGCCT 1 cut(s) 819
Eco47I GGWCC 1 cut(s) 776
Eco57I CTGAAG 1 cut(s) 858
EcoNI CCTNNNNNAGG 1 cut(s) 530
EcoRII CCWGG 1 cut(s) 709
EcoT14I CCWWGG 1 cut(s) 433
EcoT22I ATGCAT 1 cut(s) 6
ErhI CCWWGG 1 cut(s) 433
FaeI CATG 2 cut(s) 8, 584
FaiI YATR 7 cut(s) 6, 410, 461, 582, 617, 657, 717
FalI AAGNNNNNCTT 4 cut(s) 630, 662, 756, 788
FatI CATG 2 cut(s) 4, 580
Fnu4HI GCNGC 3 cut(s) 288, 642, 768
FokI GGATG 2 cut(s) 584, 615
Fsp4HI GCNGC 3 cut(s) 288, 642, 768
FspBI CTAG 5 cut(s) 338, 357, 509, 551, 566
GlaI GCGC 1 cut(s) 153
GluI GCNGC 3 cut(s) 288, 642, 768
GsaI CCCAGC 1 cut(s) 56
GsuI CTGGAG 2 cut(s) 181, 207
HaeIII GGCC 4 cut(s) 63, 469, 607, 819
HapII CCGG 2 cut(s) 34, 832
HhaI GCGC 1 cut(s) 154
Hin1I GRCGYC 1 cut(s) 132
Hin1II CATG 2 cut(s) 8, 584
Hin6I GCGC 1 cut(s) 152
HinP1I GCGC 1 cut(s) 152
HincII GTYRAC 2 cut(s) 418, 846
HindII GTYRAC 2 cut(s) 418, 846
HindIII AAGCTT 1 cut(s) 401
HinfI GANTC 6 cut(s) 429, 584, 593, 663, 748, 842
HpaI GTTAAC 1 cut(s) 418
HpaII CCGG 2 cut(s) 34, 832
HphI GGTGA 1 cut(s) 520
Hpy166II GTNNAC 3 cut(s) 418, 544, 846
Hpy188I TCNGA 3 cut(s) 41, 319, 398
Hpy188III TCNNGA 1 cut(s) 566
Hpy8I GTNNAC 3 cut(s) 418, 544, 846
Hpy99I CGWCG 1 cut(s) 134
HpyAV CCTTC 2 cut(s) 89, 496
HpyCH4III ACNGT 1 cut(s) 757
HpyCH4IV ACGT 1 cut(s) 132
HpyCH4V TGCA 4 cut(s) 4, 123, 176, 770
HpyF10VI GCNNNNNNNGC 5 cut(s) 266, 371, 650, 698, 797
HpySE526I ACGT 1 cut(s) 132
Hsp92I GRCGYC 1 cut(s) 132
Hsp92II CATG 2 cut(s) 8, 584
HspAI GCGC 1 cut(s) 152
KspAI GTTAAC 1 cut(s) 418
KspI CCGCGG 1 cut(s) 86
Kzo9I GATC 2 cut(s) 437, 727
LmnI GCTCC 3 cut(s) 85, 188, 505
Lsp1109I GCAGC 3 cut(s) 299, 653, 754
LweI GCATC 2 cut(s) 256, 505
MaeI CTAG 5 cut(s) 338, 357, 509, 551, 566
MaeII ACGT 1 cut(s) 132
MaeIII GTNAC 2 cut(s) 181, 343
MalI GATC 2 cut(s) 439, 729
MboI GATC 2 cut(s) 437, 727
MboII GAAGA 7 cut(s) 104, 111, 283, 319, 773, 814, 851
MflI RGATCY 2 cut(s) 437, 727
MluCI AATT 4 cut(s) 224, 392, 560, 810
MlyI GAGTC 3 cut(s) 438, 672, 851
MmeI TCCRAC 1 cut(s) 19
Mph1103I ATGCAT 1 cut(s) 6
MseI TTAA 5 cut(s) 254, 417, 648, 696, 856
MslI CAYNNNNRTG 1 cut(s) 140
MspA1I CMGCKG 2 cut(s) 85, 290
MspI CCGG 2 cut(s) 34, 832
MspR9I CCNGG 2 cut(s) 35, 711
MvaI CCWGG 1 cut(s) 711
MvnI CGCG 2 cut(s) 85, 154
MwoI GCNNNNNNNGC 5 cut(s) 266, 371, 650, 698, 797
NciI CCSGG 1 cut(s) 35
NdeII GATC 2 cut(s) 437, 727
NlaIII CATG 2 cut(s) 8, 584
NlaIV GGNNCC 1 cut(s) 190
NmeAIII GCCGAG 1 cut(s) 89
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 1 cut(s) 8
PceI AGGCCT 1 cut(s) 819
PcsI WCGNNNNNNNCGW 1 cut(s) 45
PfeI GAWTC 3 cut(s) 584, 593, 748
PflFI GACNNNGTC 1 cut(s) 35
PflMI CCANNNNNTGG 1 cut(s) 603
PkrI GCNGC 3 cut(s) 289, 643, 769
PleI GAGTC 3 cut(s) 437, 671, 850
PpsI GAGTC 3 cut(s) 437, 671, 850
Psp6I CCWGG 1 cut(s) 709
PspFI CCCAGC 1 cut(s) 52
PspGI CCWGG 1 cut(s) 709
PspN4I GGNNCC 1 cut(s) 190
PspPI GGNCC 3 cut(s) 61, 467, 776
PsuI RGATCY 2 cut(s) 437, 727
PsyI GACNNNGTC 1 cut(s) 35
PvuII CAGCTG 1 cut(s) 290
RseI CAYNNNNRTG 1 cut(s) 140
SacII CCGCGG 1 cut(s) 86
SaqAI TTAA 5 cut(s) 254, 417, 648, 696, 856
SatI GCNGC 3 cut(s) 288, 642, 768
Sau3AI GATC 2 cut(s) 437, 727
Sau96I GGNCC 3 cut(s) 61, 467, 776
SchI GAGTC 3 cut(s) 438, 672, 851
ScrFI CCNGG 2 cut(s) 35, 711
SfaNI GCATC 2 cut(s) 256, 505
Sfr303I CCGCGG 1 cut(s) 86
SgrBI CCGCGG 1 cut(s) 86
SinI GGWCC 1 cut(s) 776
SmiMI CAYNNNNRTG 1 cut(s) 140
SmlI CTYRAG 1 cut(s) 209
SmoI CTYRAG 1 cut(s) 209
Sse9I AATT 4 cut(s) 224, 392, 560, 810
SseBI AGGCCT 1 cut(s) 819
SsiI CCGC 4 cut(s) 83, 85, 154, 518
SspMI CTAG 5 cut(s) 338, 357, 509, 551, 566
StuI AGGCCT 1 cut(s) 819
StyD4I CCNGG 2 cut(s) 33, 709
StyI CCWWGG 1 cut(s) 433
TaaI ACNGT 1 cut(s) 757
TaiI ACGT 1 cut(s) 135
TaqI TCGA 2 cut(s) 587, 635
TasI AATT 4 cut(s) 224, 392, 560, 810
TfiI GAWTC 3 cut(s) 584, 593, 748
Tru1I TTAA 5 cut(s) 254, 417, 648, 696, 856
Tru9I TTAA 5 cut(s) 254, 417, 648, 696, 856
TseI GCWGC 3 cut(s) 287, 641, 767
Tth111I GACNNNGTC 1 cut(s) 35
Van91I CCANNNNNTGG 1 cut(s) 603
VpaK11BI GGWCC 1 cut(s) 776
XagI CCTNNNNNAGG 1 cut(s) 530
XapI RAATTY 2 cut(s) 560, 810
XbaI TCTAGA 1 cut(s) 565
XceI RCATGY 1 cut(s) 8
XspI CTAG 5 cut(s) 338, 357, 509, 551, 566
ZraI GACGTC 1 cut(s) 133
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.