Rmu_sc0001250.1_g000001

Salutaridine reductase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001250.1
OG0001077
Physical Location & Seq
Forward (+)
1545 .. 5823
4279 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0001250.1_g000001.1.cds

Sequence Viewer

Length: 756 bp
atgcatgtgagagaggagagagagagagctgaccgggtcggacagagcgagctgggattgggccgagctgtgagggaagctccgcggagagaagaaggaagaaaggaagttgctggcaagtgcagaaacgacgtcatttcaatggaggttgcgcggtgctggagtggtggaagcaagtcggaaggtgtttttctgaagcagttaggaggtatacaagttaaacaaaataaagtgcaaacttacgagacagcggcggattgtttgaaaacaaactattatggaatcaagcaactcacagaagcacttgttccactacttcaaaaatctgaagctccaaagatagtcaatgtctcttcctcacggggacagctgagacttatttcaaatgagaaagcaaagaaggagctaggagatgtggataacctcaccgaggagaaagtggacaagctagtcgaggagtttctagaggatgtgaagcatgattcgataaaatccaaaggatggcctttagatatatctgcctacattgtatcaaaagcagctcttaatgcttatacaagagtcgtggcaaagaagtatccccacattgctataaacgcagttggtccaggttataccaaaacagatctcaacaataataacgggattctcacagttgaagaagctgcactaggtcctgtgaagctggctttgatagccgaaactggaatttcaggccgcttctttaaccggaatgaagagtcaacctttgattga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00902 ko01110 map00902 map01110
Pfam Domains
Protein Families
adh_short adh_short_C2

Protein Analysis

251

Amino Acids

27.78

Weight (kDa)

8.61

Isoelectric Point (pI)

36.65

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 449
AatII GACGTC 1 cut(s) 135
AccB7I CCANNNNNTGG 1 cut(s) 501
AccI GTMKAC 1 cut(s) 211
AccII CGCG 2 cut(s) 85, 154
AciI CCGC 6 cut(s) 83, 85, 154, 251, 254, 718
AcsI RAATTY 1 cut(s) 708
AcuI CTGAAG 2 cut(s) 215, 348
AcyI GRCGYC 1 cut(s) 132
AfiI CCNNNNNNNGG 2 cut(s) 430, 501
AgsI TTSAA 5 cut(s) 141, 265, 320, 384, 659
AjnI CCWGG 1 cut(s) 607
Alw26I GTCTC 3 cut(s) 239, 355, 367
AoxI GGCC 3 cut(s) 61, 503, 715
ApeKI GCWGC 2 cut(s) 539, 665
ApoI RAATTY 1 cut(s) 708
AspLEI GCGC 1 cut(s) 154
AspS9I GGNCC 3 cut(s) 61, 605, 674
AsuC2I CCSGG 1 cut(s) 35
AsuHPI GGTGA 1 cut(s) 418
AvaII GGWCC 2 cut(s) 605, 674
BbvI GCAGC 2 cut(s) 551, 652
BccI CCATC 1 cut(s) 495
BciT130I CCWGG 1 cut(s) 609
BciVI GTATCC 1 cut(s) 588
BcnI CCSGG 1 cut(s) 35
BcoDI GTCTC 3 cut(s) 239, 355, 367
BfaI CTAG 4 cut(s) 407, 449, 464, 671
BfuI GTATCC 1 cut(s) 588
BglII AGATCT 1 cut(s) 625
BisI GCNGC 4 cut(s) 252, 540, 666, 718
BlsI GCNGC 4 cut(s) 253, 541, 667, 719
Bme1390I CCNGG 2 cut(s) 35, 609
Bme18I GGWCC 2 cut(s) 605, 674
BmgT120I GGNCC 3 cut(s) 61, 605, 674
BmrFI CCNGG 2 cut(s) 35, 609
BplI GAGNNNNNCTC 2 cut(s) 64, 96
BpmI CTGGAG 1 cut(s) 181
BpuMI CCSGG 1 cut(s) 35
BsaHI GRCGYC 1 cut(s) 132
BsaJI CCNNGG 2 cut(s) 83, 429
BsaWI WCCGGW 1 cut(s) 729
Bsc4I CCNNNNNNNGG 2 cut(s) 430, 501
Bse1I ACTGG 1 cut(s) 709
Bse3DI GCAATG 1 cut(s) 585
BseBI CCWGG 1 cut(s) 609
BseDI CCNNGG 2 cut(s) 83, 429
BseGI GGATG 2 cut(s) 475, 506
BseLI CCNNNNNNNGG 2 cut(s) 430, 501
BseMI GCAATG 1 cut(s) 585
BseMII CTCAG 1 cut(s) 362
BseNI ACTGG 1 cut(s) 709
BseRI GAGGAG 3 cut(s) 29, 446, 470
BseXI GCAGC 2 cut(s) 551, 652
BseYI CCCAGC 1 cut(s) 52
BsgI GTGCAG 2 cut(s) 142, 651
Bsh1236I CGCG 2 cut(s) 85, 154
BshFI GGCC 3 cut(s) 63, 505, 717
BsiSI CCGG 2 cut(s) 34, 730
BslFI GGGAC 1 cut(s) 378
BslI CCNNNNNNNGG 2 cut(s) 430, 501
BsmAI GTCTC 3 cut(s) 239, 355, 367
BsmFI GGGAC 1 cut(s) 378
BsnI GGCC 3 cut(s) 63, 505, 717
Bsp143I GATC 1 cut(s) 625
BspACI CCGC 6 cut(s) 83, 85, 154, 251, 254, 718
BspANI GGCC 3 cut(s) 63, 505, 717
BspCNI CTCAG 1 cut(s) 363
BspFNI CGCG 2 cut(s) 85, 154
BsrDI GCAATG 1 cut(s) 585
BsrI ACTGG 1 cut(s) 709
BssECI CCNNGG 2 cut(s) 83, 429
BssMI GATC 1 cut(s) 625
BssNAI GTATAC 1 cut(s) 212
BssNI GRCGYC 1 cut(s) 132
Bst1107I GTATAC 1 cut(s) 212
Bst2UI CCWGG 1 cut(s) 609
Bst4CI ACNGT 1 cut(s) 655
Bst6I CTCTTC 2 cut(s) 358, 732
BstACI GRCGYC 1 cut(s) 132
BstC8I GCNNGC 3 cut(s) 50, 115, 687
BstDEI CTNAG 1 cut(s) 371
BstDSI CCRYGG 1 cut(s) 83
BstENI CCTNNNNNAGG 1 cut(s) 428
BstF5I GGATG 2 cut(s) 475, 506
BstFNI CGCG 2 cut(s) 85, 154
BstHHI GCGC 1 cut(s) 154
BstKTI GATC 1 cut(s) 628
BstMAI GTCTC 3 cut(s) 239, 355, 367
BstMBI GATC 1 cut(s) 625
BstMWI GCNNNNNNNGC 3 cut(s) 548, 596, 695
BstNI CCWGG 1 cut(s) 609
BstNSI RCATGY 1 cut(s) 8
BstSCI CCNGG 2 cut(s) 33, 607
BstUI CGCG 2 cut(s) 85, 154
BstV1I GCAGC 2 cut(s) 551, 652
BstX2I RGATCY 1 cut(s) 625
BstYI RGATCY 1 cut(s) 625
BstZ17I GTATAC 1 cut(s) 212
BsuI GTATCC 1 cut(s) 588
BsuRI GGCC 3 cut(s) 63, 505, 717
BtgI CCRYGG 1 cut(s) 83
BtsCI GGATG 2 cut(s) 475, 506
Cac8I GCNNGC 3 cut(s) 50, 115, 687
CfoI GCGC 1 cut(s) 154
Cfr13I GGNCC 3 cut(s) 61, 605, 674
Cfr42I CCGCGG 1 cut(s) 86
CspCI CAANNNNNGTGG 2 cut(s) 546, 581
CviAII CATG 2 cut(s) 5, 479
DdeI CTNAG 1 cut(s) 371
DpnI GATC 1 cut(s) 627
DpnII GATC 1 cut(s) 625
DrdI GACNNNNNNGTC 1 cut(s) 449
DseDI GACNNNNNNGTC 1 cut(s) 449
Eam1104I CTCTTC 2 cut(s) 358, 732
EarI CTCTTC 2 cut(s) 358, 732
EciI GGCGGA 1 cut(s) 269
Eco47I GGWCC 2 cut(s) 605, 674
Eco57I CTGAAG 2 cut(s) 215, 348
EcoNI CCTNNNNNAGG 1 cut(s) 428
EcoO109I RGGNCCY 1 cut(s) 674
EcoRII CCWGG 1 cut(s) 607
EcoT22I ATGCAT 1 cut(s) 6
FaeI CATG 2 cut(s) 8, 482
FaiI YATR 8 cut(s) 6, 212, 279, 480, 515, 555, 593, 615
FalI AAGNNNNNCTT 2 cut(s) 528, 560
FaqI GGGAC 1 cut(s) 378
FatI CATG 2 cut(s) 4, 478
FblI GTMKAC 1 cut(s) 211
Fnu4HI GCNGC 4 cut(s) 252, 540, 666, 718
FokI GGATG 2 cut(s) 482, 513
Fsp4HI GCNGC 4 cut(s) 252, 540, 666, 718
FspBI CTAG 4 cut(s) 407, 449, 464, 671
GlaI GCGC 1 cut(s) 153
GluI GCNGC 4 cut(s) 252, 540, 666, 718
GsaI CCCAGC 1 cut(s) 56
GsuI CTGGAG 1 cut(s) 181
HaeIII GGCC 3 cut(s) 63, 505, 717
HapII CCGG 2 cut(s) 34, 730
HhaI GCGC 1 cut(s) 154
Hin1I GRCGYC 1 cut(s) 132
Hin1II CATG 2 cut(s) 8, 482
Hin6I GCGC 1 cut(s) 152
HinP1I GCGC 1 cut(s) 152
HincII GTYRAC 1 cut(s) 744
HindII GTYRAC 1 cut(s) 744
HinfI GANTC 5 cut(s) 282, 482, 561, 646, 740
HpaII CCGG 2 cut(s) 34, 730
HphI GGTGA 1 cut(s) 418
Hpy166II GTNNAC 3 cut(s) 212, 442, 744
Hpy188I TCNGA 4 cut(s) 41, 181, 195, 328
Hpy188III TCNNGA 1 cut(s) 464
Hpy8I GTNNAC 3 cut(s) 212, 442, 744
Hpy99I CGWCG 1 cut(s) 134
HpyAV CCTTC 3 cut(s) 89, 176, 394
HpyCH4III ACNGT 1 cut(s) 655
HpyCH4IV ACGT 1 cut(s) 132
HpyCH4V TGCA 4 cut(s) 4, 123, 235, 668
HpyF10VI GCNNNNNNNGC 3 cut(s) 548, 596, 695
HpyF3I CTNAG 1 cut(s) 371
HpySE526I ACGT 1 cut(s) 132
Hsp92I GRCGYC 1 cut(s) 132
Hsp92II CATG 2 cut(s) 8, 482
HspAI GCGC 1 cut(s) 152
KspI CCGCGG 1 cut(s) 86
Kzo9I GATC 1 cut(s) 625
LmnI GCTCC 3 cut(s) 85, 337, 403
Lsp1109I GCAGC 2 cut(s) 551, 652
MaeI CTAG 4 cut(s) 407, 449, 464, 671
MaeII ACGT 1 cut(s) 132
MalI GATC 1 cut(s) 627
MboI GATC 1 cut(s) 625
MboII GAAGA 5 cut(s) 104, 111, 345, 671, 749
MflI RGATCY 1 cut(s) 625
MluCI AATT 1 cut(s) 708
MlyI GAGTC 2 cut(s) 570, 749
MmeI TCCRAC 2 cut(s) 19, 159
MnlI CCTC 9 cut(s) 7, 66, 139, 200, 367, 424, 434, 448, 460
Mph1103I ATGCAT 1 cut(s) 6
MseI TTAA 3 cut(s) 219, 546, 726
MslI CAYNNNNRTG 1 cut(s) 140
MspA1I CMGCKG 3 cut(s) 85, 251, 370
MspI CCGG 2 cut(s) 34, 730
MspR9I CCNGG 2 cut(s) 35, 609
MvaI CCWGG 1 cut(s) 609
MvnI CGCG 2 cut(s) 85, 154
MwoI GCNNNNNNNGC 3 cut(s) 548, 596, 695
NciI CCSGG 1 cut(s) 35
NdeII GATC 1 cut(s) 625
NlaIII CATG 2 cut(s) 8, 482
NmeAIII GCCGAG 1 cut(s) 89
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 1 cut(s) 8
PcsI WCGNNNNNNNCGW 1 cut(s) 45
PfeI GAWTC 3 cut(s) 282, 482, 646
PflFI GACNNNGTC 1 cut(s) 35
PflMI CCANNNNNTGG 1 cut(s) 501
PkrI GCNGC 4 cut(s) 253, 541, 667, 719
PleI GAGTC 2 cut(s) 569, 748
PpsI GAGTC 2 cut(s) 569, 748
PpuMI RGGWCCY 1 cut(s) 674
Psp5II RGGWCCY 1 cut(s) 674
Psp6I CCWGG 1 cut(s) 607
PspFI CCCAGC 1 cut(s) 52
PspGI CCWGG 1 cut(s) 607
PspPI GGNCC 3 cut(s) 61, 605, 674
PspPPI RGGWCCY 1 cut(s) 674
PsuI RGATCY 1 cut(s) 625
PsyI GACNNNGTC 1 cut(s) 35
PvuII CAGCTG 1 cut(s) 370
RseI CAYNNNNRTG 1 cut(s) 140
SacII CCGCGG 1 cut(s) 86
SaqAI TTAA 3 cut(s) 219, 546, 726
SatI GCNGC 4 cut(s) 252, 540, 666, 718
Sau3AI GATC 1 cut(s) 625
Sau96I GGNCC 3 cut(s) 61, 605, 674
SchI GAGTC 2 cut(s) 570, 749
ScrFI CCNGG 2 cut(s) 35, 609
Sfr303I CCGCGG 1 cut(s) 86
SgrBI CCGCGG 1 cut(s) 86
SinI GGWCC 2 cut(s) 605, 674
SmiMI CAYNNNNRTG 1 cut(s) 140
Sse9I AATT 1 cut(s) 708
SsiI CCGC 6 cut(s) 83, 85, 154, 251, 254, 718
SspMI CTAG 4 cut(s) 407, 449, 464, 671
StyD4I CCNGG 2 cut(s) 33, 607
TaaI ACNGT 1 cut(s) 655
TaiI ACGT 1 cut(s) 135
TaqI TCGA 2 cut(s) 453, 485
TasI AATT 1 cut(s) 708
TauI GCSGC 2 cut(s) 254, 720
TfiI GAWTC 3 cut(s) 282, 482, 646
Tru1I TTAA 3 cut(s) 219, 546, 726
Tru9I TTAA 3 cut(s) 219, 546, 726
TseI GCWGC 2 cut(s) 539, 665
TspDTI ATGAA 1 cut(s) 750
Tth111I GACNNNGTC 1 cut(s) 35
Van91I CCANNNNNTGG 1 cut(s) 501
VpaK11BI GGWCC 2 cut(s) 605, 674
XagI CCTNNNNNAGG 1 cut(s) 428
XapI RAATTY 1 cut(s) 708
XbaI TCTAGA 1 cut(s) 463
XceI RCATGY 1 cut(s) 8
XmiI GTMKAC 1 cut(s) 211
XspI CTAG 4 cut(s) 407, 449, 464, 671
ZraI GACGTC 1 cut(s) 133
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.