Rroxscaffold_3G00247240

component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0008374
Physical Location & Seq
Forward (+)
40622745 .. 40626255
3511 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00247240.1

Sequence Viewer

Length: 750 bp
ATGGAGGATTGGGAAGAAGATGCGAGAATTCCATCCCTCCTTTCTAAGGAGCCAGCAGTAAAAGGTAACTGGGATGATGAAGATGCAGATGAGAATGACGTGAAGGACTCATGGGAGGATGATGATGAACCTGCTCCTCCGGAACCTACACCAAAGGCTCCTGCTGAAAAGGCCCCCAAGAAACCTGCAACAAAGGCTGCAGAAAAGAAAGGAAAAGCTGTTGAAGTACAAAAGGAAGAGCCACTAGATTCCGTGGCTGAGAAACTTCGCCAACAAAGACTGGTGGAAGAAGCAGATTATAAGGCCACTAAAGAACTGTTTTCTACTAGAGATGAGAAGAACATTGATAATTTCATTCCCAAATCCGAAAGTGACTTCCTGGAATATGCCGAACTTATTTCACATAAACTTCGTCCATTTGAGAAAAGTTTTCATTATATCGGTCTACTTAAGGCAGTAATGAGACTGTCAATGACTTCTTTGAAAGGAGCTGATGCAAAAGATGTTGCATCTTCAATTACGGCAATTGCAAATGAGAAGATAAAAGCTGAGAAGGAAGCCAATGCCGGTAAAAAGAAGACAGGTGCCAAGAAAAAGCAGCTTCATGTTGATAAGCCGGATGATGATATAGCTGTTGATGGCTATGATCCCCTTGATGACTATGACTTTATACATGTTCAGTCATTCAATGGATCAGACCTTCTGGCTAGAGGTTCTCTCGCTTCTCTTTGCCAAAAGTTGTGGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
eIF3_subunit

Protein Analysis

249

Amino Acids

27.89

Weight (kDa)

4.95

Isoelectric Point (pI)

34.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
eIF3_subunit PF08597 2 - 224 4e-51 Translation initiation factor eIF3 subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 300
Acc36I ACCTGC 2 cut(s) 139, 193
AccB1I GGYRCC 1 cut(s) 584
AccI GTMKAC 1 cut(s) 445
AccIII TCCGGA 1 cut(s) 139
AclWI GGATC 2 cut(s) 641, 700
AcsI RAATTY 1 cut(s) 27
AfaI GTAC 1 cut(s) 228
AfiI CCNNNNNNNGG 1 cut(s) 46
AflII CTTAAG 1 cut(s) 449
AflIII ACRYGT 1 cut(s) 673
AgsI TTSAA 4 cut(s) 224, 484, 516, 688
AjiI CACGTC 1 cut(s) 100
AjnI CCWGG 1 cut(s) 378
AluBI AGCT 5 cut(s) 218, 491, 548, 601, 632
AluI AGCT 5 cut(s) 218, 491, 548, 601, 632
Alw26I GTCTC 1 cut(s) 457
AlwI GGATC 2 cut(s) 641, 700
Aor13HI TCCGGA 1 cut(s) 139
AoxI GGCC 2 cut(s) 171, 303
ApeKI GCWGC 2 cut(s) 197, 598
ApoI RAATTY 1 cut(s) 27
AspS9I GGNCC 1 cut(s) 172
BanI GGYRCC 1 cut(s) 584
BbsI GAAGAC 1 cut(s) 584
BbvI GCAGC 2 cut(s) 184, 610
BccI CCATC 2 cut(s) 40, 632
BceAI ACGGC 1 cut(s) 537
BciT130I CCWGG 1 cut(s) 380
BcoDI GTCTC 1 cut(s) 457
BfaI CTAG 3 cut(s) 245, 327, 708
BfmI CTRYAG 1 cut(s) 198
BfrI CTTAAG 1 cut(s) 449
BfuAI ACCTGC 2 cut(s) 139, 193
BisI GCNGC 2 cut(s) 198, 599
BlsI GCNGC 2 cut(s) 199, 600
Bme1390I CCNGG 1 cut(s) 380
BmgBI CACGTC 1 cut(s) 100
BmgT120I GGNCC 1 cut(s) 172
BmiI GGNNCC 5 cut(s) 51, 144, 159, 174, 586
BmrFI CCNGG 1 cut(s) 380
BmrI ACTGGG 1 cut(s) 79
BmsI GCATC 4 cut(s) 10, 73, 484, 518
BmuI ACTGGG 1 cut(s) 79
BpiI GAAGAC 1 cut(s) 584
BplI GAGNNNNNCTC 2 cut(s) 702, 734
BsaJI CCNNGG 1 cut(s) 252
BsaWI WCCGGW 1 cut(s) 139
Bsc4I CCNNNNNNNGG 1 cut(s) 46
Bse118I RCCGGY 1 cut(s) 566
Bse1I ACTGG 2 cut(s) 74, 285
BseAI TCCGGA 1 cut(s) 139
BseBI CCWGG 1 cut(s) 380
BseDI CCNNGG 1 cut(s) 252
BseGI GGATG 4 cut(s) 32, 79, 124, 625
BseLI CCNNNNNNNGG 1 cut(s) 46
BseMII CTCAG 2 cut(s) 249, 540
BseNI ACTGG 2 cut(s) 74, 285
BseRI GAGGAG 1 cut(s) 126
BseXI GCAGC 2 cut(s) 184, 610
BshFI GGCC 2 cut(s) 173, 305
BshNI GGYRCC 1 cut(s) 584
BsiSI CCGG 3 cut(s) 140, 567, 617
BslI CCNNNNNNNGG 1 cut(s) 46
BsmAI GTCTC 1 cut(s) 457
BsnI GGCC 2 cut(s) 173, 305
Bsp13I TCCGGA 1 cut(s) 139
Bsp143I GATC 2 cut(s) 646, 692
BspANI GGCC 2 cut(s) 173, 305
BspCNI CTCAG 2 cut(s) 250, 541
BspEI TCCGGA 1 cut(s) 139
BspLI GGNNCC 5 cut(s) 51, 144, 159, 174, 586
BspMAI CTGCAG 1 cut(s) 202
BspMI ACCTGC 2 cut(s) 139, 193
BspPI GGATC 2 cut(s) 641, 700
BspQI GCTCTTC 1 cut(s) 231
BspT107I GGYRCC 1 cut(s) 584
BspTI CTTAAG 1 cut(s) 449
BsrFI RCCGGY 1 cut(s) 566
BsrI ACTGG 2 cut(s) 74, 285
BssAI RCCGGY 1 cut(s) 566
BssECI CCNNGG 1 cut(s) 252
BssMI GATC 2 cut(s) 646, 692
Bst2UI CCWGG 1 cut(s) 380
Bst4CI ACNGT 2 cut(s) 318, 468
Bst6I CTCTTC 1 cut(s) 231
BstAFI CTTAAG 1 cut(s) 449
BstC8I GCNNGC 1 cut(s) 54
BstDEI CTNAG 3 cut(s) 45, 258, 549
BstDSI CCRYGG 1 cut(s) 252
BstENI CCTNNNNNAGG 1 cut(s) 44
BstF5I GGATG 4 cut(s) 32, 79, 124, 625
BstKTI GATC 2 cut(s) 649, 695
BstMAI GTCTC 1 cut(s) 457
BstMBI GATC 2 cut(s) 646, 692
BstMWI GCNNNNNNNGC 2 cut(s) 170, 194
BstNI CCWGG 1 cut(s) 380
BstNSI RCATGY 1 cut(s) 677
BstSCI CCNGG 1 cut(s) 378
BstSFI CTRYAG 1 cut(s) 198
BstV1I GCAGC 2 cut(s) 184, 610
BstV2I GAAGAC 1 cut(s) 584
BsuRI GGCC 2 cut(s) 173, 305
BtgI CCRYGG 1 cut(s) 252
BtrI CACGTC 1 cut(s) 100
BtsCI GGATG 4 cut(s) 32, 79, 124, 625
BveI ACCTGC 2 cut(s) 139, 193
Cac8I GCNNGC 1 cut(s) 54
Cfr10I RCCGGY 1 cut(s) 566
Cfr13I GGNCC 1 cut(s) 172
Csp6I GTAC 1 cut(s) 227
CspCI CAANNNNNGTGG 1 cut(s) 722
CviAII CATG 3 cut(s) 111, 605, 674
CviQI GTAC 1 cut(s) 227
DdeI CTNAG 3 cut(s) 45, 258, 549
DpnI GATC 2 cut(s) 648, 694
DpnII GATC 2 cut(s) 646, 692
Eam1104I CTCTTC 1 cut(s) 231
EarI CTCTTC 1 cut(s) 231
EcoNI CCTNNNNNAGG 1 cut(s) 44
EcoO109I RGGNCCY 1 cut(s) 172
EcoRI GAATTC 1 cut(s) 27
EcoRII CCWGG 1 cut(s) 378
FaeI CATG 3 cut(s) 114, 608, 677
FatI CATG 3 cut(s) 110, 604, 673
FblI GTMKAC 1 cut(s) 445
Fnu4HI GCNGC 2 cut(s) 198, 599
FokI GGATG 4 cut(s) 19, 86, 131, 632
Fsp4HI GCNGC 2 cut(s) 198, 599
FspBI CTAG 3 cut(s) 245, 327, 708
GluI GCNGC 2 cut(s) 198, 599
HaeIII GGCC 2 cut(s) 173, 305
HapII CCGG 3 cut(s) 140, 567, 617
Hin1II CATG 3 cut(s) 114, 608, 677
HinfI GANTC 2 cut(s) 107, 248
HpaII CCGG 3 cut(s) 140, 567, 617
Hpy166II GTNNAC 1 cut(s) 446
Hpy188I TCNGA 2 cut(s) 367, 697
Hpy188III TCNNGA 1 cut(s) 140
Hpy8I GTNNAC 1 cut(s) 446
HpyAV CCTTC 3 cut(s) 97, 547, 710
HpyCH4III ACNGT 2 cut(s) 318, 468
HpyCH4IV ACGT 1 cut(s) 99
HpyCH4V TGCA 6 cut(s) 86, 188, 200, 497, 509, 530
HpyF10VI GCNNNNNNNGC 2 cut(s) 170, 194
HpyF3I CTNAG 3 cut(s) 45, 258, 549
HpySE526I ACGT 1 cut(s) 99
Hsp92II CATG 3 cut(s) 114, 608, 677
Kpn2I TCCGGA 1 cut(s) 139
Kzo9I GATC 2 cut(s) 646, 692
LguI GCTCTTC 1 cut(s) 231
LmnI GCTCC 4 cut(s) 49, 139, 163, 488
Lsp1109I GCAGC 2 cut(s) 184, 610
LweI GCATC 4 cut(s) 10, 73, 484, 518
MaeI CTAG 3 cut(s) 245, 327, 708
MaeII ACGT 1 cut(s) 99
MaeIII GTNAC 2 cut(s) 65, 371
MalI GATC 2 cut(s) 648, 694
MboI GATC 2 cut(s) 646, 692
MboII GAAGA 9 cut(s) 26, 29, 92, 248, 299, 349, 504, 550, 589
MfeI CAATTG 1 cut(s) 525
MluCI AATT 4 cut(s) 27, 349, 516, 525
MlyI GAGTC 1 cut(s) 101
MnlI CCTC 4 cut(s) 47, 109, 147, 704
MroI TCCGGA 1 cut(s) 139
MseI TTAA 1 cut(s) 450
MspCI CTTAAG 1 cut(s) 449
MspI CCGG 3 cut(s) 140, 567, 617
MspR9I CCNGG 1 cut(s) 380
MunI CAATTG 1 cut(s) 525
MvaI CCWGG 1 cut(s) 380
MwoI GCNNNNNNNGC 2 cut(s) 170, 194
NdeII GATC 2 cut(s) 646, 692
NlaIII CATG 3 cut(s) 114, 608, 677
NlaIV GGNNCC 5 cut(s) 51, 144, 159, 174, 586
NmuCI GTSAC 1 cut(s) 371
NspI RCATGY 1 cut(s) 677
PciI ACATGT 1 cut(s) 673
PciSI GCTCTTC 1 cut(s) 231
PfeI GAWTC 1 cut(s) 248
PfoI TCCNGGA 1 cut(s) 378
PkrI GCNGC 2 cut(s) 199, 600
PleI GAGTC 1 cut(s) 101
PpsI GAGTC 1 cut(s) 101
PscI ACATGT 1 cut(s) 673
PsiI TTATAA 1 cut(s) 300
Psp6I CCWGG 1 cut(s) 378
PspGI CCWGG 1 cut(s) 378
PspN4I GGNNCC 5 cut(s) 51, 144, 159, 174, 586
PspPI GGNCC 1 cut(s) 172
PstI CTGCAG 1 cut(s) 202
RsaI GTAC 1 cut(s) 228
RsaNI GTAC 1 cut(s) 227
SapI GCTCTTC 1 cut(s) 231
SaqAI TTAA 1 cut(s) 450
SatI GCNGC 2 cut(s) 198, 599
Sau3AI GATC 2 cut(s) 646, 692
Sau96I GGNCC 1 cut(s) 172
SchI GAGTC 1 cut(s) 101
ScrFI CCNGG 1 cut(s) 380
SfaNI GCATC 4 cut(s) 10, 73, 484, 518
SfcI CTRYAG 1 cut(s) 198
SmlI CTYRAG 1 cut(s) 449
SmoI CTYRAG 1 cut(s) 449
Sse9I AATT 4 cut(s) 27, 349, 516, 525
SspMI CTAG 3 cut(s) 245, 327, 708
StyD4I CCNGG 1 cut(s) 378
TaaI ACNGT 2 cut(s) 318, 468
TaiI ACGT 1 cut(s) 102
TaqII GACCGA 1 cut(s) 431
TasI AATT 4 cut(s) 27, 349, 516, 525
TatI WGTACW 1 cut(s) 226
TfiI GAWTC 1 cut(s) 248
Tru1I TTAA 1 cut(s) 450
Tru9I TTAA 1 cut(s) 450
TseFI GTSAC 1 cut(s) 371
TseI GCWGC 2 cut(s) 197, 598
Tsp45I GTSAC 1 cut(s) 371
TspDTI ATGAA 5 cut(s) 93, 141, 343, 422, 593
TspGWI ACGGA 1 cut(s) 241
Vha464I CTTAAG 1 cut(s) 449
XagI CCTNNNNNAGG 1 cut(s) 44
XapI RAATTY 1 cut(s) 27
XceI RCATGY 1 cut(s) 677
XmiI GTMKAC 1 cut(s) 445
XspI CTAG 3 cut(s) 245, 327, 708
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.