Rmu_co8494947.1_g000001

component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8494947.1
OG0008374
Physical Location & Seq
Forward (+)
1 .. 2448
2448 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8494947.1_g000001.1.cds

Sequence Viewer

Length: 662 bp
aagaagatgcgagaattccatccctcctttctaaggagcctgcagtaaaaggtaactgggatgatgaagatgcggatgagaatgacgtgaaggactcatgggaggatgatgatgaacctgctcctccggaacctgcaccaaaggctcctgctgaaaaggctcccaagaaacctgcaacaaaggctgcagaaaagaaaggaaaagctgttgaagtacaaaaggaagagccactagatcccctggctgagaaacttcgccaacaaagactggtggaagaagcagattataaggccactaaagaactgttttctactagagatgagaagaacattgataatttcattcccaaatccgaaagtgacttcctggaatatgccgaacttatttcacataaacttcgtccatttgagaaaagttttcattatatcggtctacttaaggcagtaatgagactgtcaatgacttctttgaaaggagctgatgcaaaagatgttgcttcttcaattacggcaattgcaaatgagaagataaaagctgagaaggaagccaatgctggtaaaaagaagacaggtgccaagaaaaagcagcttcatgttgataagccggatgatgatatagctgttgatggctatgatccccttgatgactatgactttatgtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
eIF3_subunit

Protein Analysis

219

Amino Acids

24.47

Weight (kDa)

4.92

Isoelectric Point (pI)

34.76

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 287
Acc36I ACCTGC 3 cut(s) 126, 141, 180
AccB1I GGYRCC 1 cut(s) 571
AccI GTMKAC 1 cut(s) 432
AccIII TCCGGA 1 cut(s) 126
AciI CCGC 1 cut(s) 73
AclWI GGATC 2 cut(s) 229, 628
AcsI RAATTY 1 cut(s) 14
AfaI GTAC 1 cut(s) 215
AfiI CCNNNNNNNGG 1 cut(s) 33
AflII CTTAAG 1 cut(s) 436
AgsI TTSAA 3 cut(s) 211, 471, 503
AjiI CACGTC 1 cut(s) 87
AjnI CCWGG 2 cut(s) 239, 365
AluBI AGCT 5 cut(s) 205, 478, 535, 588, 619
AluI AGCT 5 cut(s) 205, 478, 535, 588, 619
Alw26I GTCTC 1 cut(s) 444
AlwI GGATC 2 cut(s) 229, 628
Aor13HI TCCGGA 1 cut(s) 126
AoxI GGCC 1 cut(s) 290
ApeKI GCWGC 2 cut(s) 184, 585
ApoI RAATTY 1 cut(s) 14
BanI GGYRCC 1 cut(s) 571
BbsI GAAGAC 1 cut(s) 571
BbvI GCAGC 2 cut(s) 171, 597
BccI CCATC 2 cut(s) 27, 619
BceAI ACGGC 1 cut(s) 524
BciT130I CCWGG 2 cut(s) 241, 367
BcoDI GTCTC 1 cut(s) 444
BfaI CTAG 2 cut(s) 232, 314
BfmI CTRYAG 2 cut(s) 41, 185
BfrI CTTAAG 1 cut(s) 436
BfuAI ACCTGC 3 cut(s) 126, 141, 180
BisI GCNGC 2 cut(s) 185, 586
BlsI GCNGC 2 cut(s) 186, 587
Bme1390I CCNGG 2 cut(s) 241, 367
BmgBI CACGTC 1 cut(s) 87
BmiI GGNNCC 5 cut(s) 38, 131, 146, 161, 573
BmrFI CCNGG 2 cut(s) 241, 367
BmrI ACTGGG 1 cut(s) 66
BmsI GCATC 2 cut(s) 60, 471
BmuI ACTGGG 1 cut(s) 66
BpiI GAAGAC 1 cut(s) 571
BsaJI CCNNGG 1 cut(s) 239
BsaWI WCCGGW 1 cut(s) 126
Bsc4I CCNNNNNNNGG 1 cut(s) 33
Bse1I ACTGG 2 cut(s) 61, 272
BseAI TCCGGA 1 cut(s) 126
BseBI CCWGG 2 cut(s) 241, 367
BseDI CCNNGG 1 cut(s) 239
BseGI GGATG 5 cut(s) 19, 66, 81, 111, 612
BseLI CCNNNNNNNGG 1 cut(s) 33
BseMII CTCAG 2 cut(s) 236, 527
BseNI ACTGG 2 cut(s) 61, 272
BseRI GAGGAG 1 cut(s) 113
BseXI GCAGC 2 cut(s) 171, 597
BsgI GTGCAG 1 cut(s) 119
BshFI GGCC 1 cut(s) 292
BshNI GGYRCC 1 cut(s) 571
BsiSI CCGG 2 cut(s) 127, 604
BslI CCNNNNNNNGG 1 cut(s) 33
BsmAI GTCTC 1 cut(s) 444
BsnI GGCC 1 cut(s) 292
Bsp13I TCCGGA 1 cut(s) 126
Bsp143I GATC 2 cut(s) 234, 633
BspACI CCGC 1 cut(s) 73
BspANI GGCC 1 cut(s) 292
BspCNI CTCAG 2 cut(s) 237, 528
BspEI TCCGGA 1 cut(s) 126
BspLI GGNNCC 5 cut(s) 38, 131, 146, 161, 573
BspMAI CTGCAG 2 cut(s) 45, 189
BspMI ACCTGC 3 cut(s) 126, 141, 180
BspPI GGATC 2 cut(s) 229, 628
BspQI GCTCTTC 1 cut(s) 218
BspT107I GGYRCC 1 cut(s) 571
BspTI CTTAAG 1 cut(s) 436
BsrI ACTGG 2 cut(s) 61, 272
BssECI CCNNGG 1 cut(s) 239
BssMI GATC 2 cut(s) 234, 633
Bst2UI CCWGG 2 cut(s) 241, 367
Bst4CI ACNGT 2 cut(s) 305, 455
Bst6I CTCTTC 1 cut(s) 218
BstAFI CTTAAG 1 cut(s) 436
BstC8I GCNNGC 1 cut(s) 41
BstDEI CTNAG 3 cut(s) 32, 245, 536
BstENI CCTNNNNNAGG 1 cut(s) 31
BstF5I GGATG 5 cut(s) 19, 66, 81, 111, 612
BstKTI GATC 2 cut(s) 237, 636
BstMAI GTCTC 1 cut(s) 444
BstMBI GATC 2 cut(s) 234, 633
BstMWI GCNNNNNNNGC 3 cut(s) 142, 157, 181
BstNI CCWGG 2 cut(s) 241, 367
BstSCI CCNGG 2 cut(s) 239, 365
BstSFI CTRYAG 2 cut(s) 41, 185
BstV1I GCAGC 2 cut(s) 171, 597
BstV2I GAAGAC 1 cut(s) 571
BstX2I RGATCY 1 cut(s) 234
BstYI RGATCY 1 cut(s) 234
BsuRI GGCC 1 cut(s) 292
BtrI CACGTC 1 cut(s) 87
BtsCI GGATG 5 cut(s) 19, 66, 81, 111, 612
BveI ACCTGC 3 cut(s) 126, 141, 180
Cac8I GCNNGC 1 cut(s) 41
Csp6I GTAC 1 cut(s) 214
CviAII CATG 2 cut(s) 98, 592
CviQI GTAC 1 cut(s) 214
DdeI CTNAG 3 cut(s) 32, 245, 536
DpnI GATC 2 cut(s) 236, 635
DpnII GATC 2 cut(s) 234, 633
Eam1104I CTCTTC 1 cut(s) 218
EarI CTCTTC 1 cut(s) 218
EcoNI CCTNNNNNAGG 1 cut(s) 31
EcoRI GAATTC 1 cut(s) 14
EcoRII CCWGG 2 cut(s) 239, 365
FaeI CATG 2 cut(s) 101, 595
FatI CATG 2 cut(s) 97, 591
FblI GTMKAC 1 cut(s) 432
Fnu4HI GCNGC 2 cut(s) 185, 586
FokI GGATG 5 cut(s) 6, 73, 88, 118, 619
Fsp4HI GCNGC 2 cut(s) 185, 586
FspBI CTAG 2 cut(s) 232, 314
GluI GCNGC 2 cut(s) 185, 586
HaeIII GGCC 1 cut(s) 292
HapII CCGG 2 cut(s) 127, 604
Hin1II CATG 2 cut(s) 101, 595
HinfI GANTC 1 cut(s) 94
HpaII CCGG 2 cut(s) 127, 604
Hpy166II GTNNAC 1 cut(s) 433
Hpy188I TCNGA 1 cut(s) 354
Hpy188III TCNNGA 1 cut(s) 127
Hpy8I GTNNAC 1 cut(s) 433
HpyAV CCTTC 2 cut(s) 84, 534
HpyCH4III ACNGT 2 cut(s) 305, 455
HpyCH4IV ACGT 1 cut(s) 86
HpyCH4V TGCA 6 cut(s) 43, 136, 175, 187, 484, 517
HpyF10VI GCNNNNNNNGC 3 cut(s) 142, 157, 181
HpyF3I CTNAG 3 cut(s) 32, 245, 536
HpySE526I ACGT 1 cut(s) 86
Hsp92II CATG 2 cut(s) 101, 595
Kpn2I TCCGGA 1 cut(s) 126
Kzo9I GATC 2 cut(s) 234, 633
LguI GCTCTTC 1 cut(s) 218
LmnI GCTCC 5 cut(s) 36, 126, 150, 165, 475
Lsp1109I GCAGC 2 cut(s) 171, 597
LweI GCATC 2 cut(s) 60, 471
MaeI CTAG 2 cut(s) 232, 314
MaeII ACGT 1 cut(s) 86
MaeIII GTNAC 2 cut(s) 52, 358
MalI GATC 2 cut(s) 236, 635
MboI GATC 2 cut(s) 234, 633
MboII GAAGA 8 cut(s) 16, 79, 235, 286, 336, 491, 537, 576
MfeI CAATTG 1 cut(s) 512
MflI RGATCY 1 cut(s) 234
MluCI AATT 4 cut(s) 14, 336, 503, 512
MlyI GAGTC 1 cut(s) 88
MnlI CCTC 3 cut(s) 34, 96, 134
MroI TCCGGA 1 cut(s) 126
MseI TTAA 1 cut(s) 437
MspCI CTTAAG 1 cut(s) 436
MspI CCGG 2 cut(s) 127, 604
MspR9I CCNGG 2 cut(s) 241, 367
MunI CAATTG 1 cut(s) 512
MvaI CCWGG 2 cut(s) 241, 367
MwoI GCNNNNNNNGC 3 cut(s) 142, 157, 181
NdeII GATC 2 cut(s) 234, 633
NlaIII CATG 2 cut(s) 101, 595
NlaIV GGNNCC 5 cut(s) 38, 131, 146, 161, 573
NmuCI GTSAC 1 cut(s) 358
PciSI GCTCTTC 1 cut(s) 218
PfoI TCCNGGA 1 cut(s) 365
PkrI GCNGC 2 cut(s) 186, 587
PleI GAGTC 1 cut(s) 88
PpsI GAGTC 1 cut(s) 88
PsiI TTATAA 1 cut(s) 287
Psp6I CCWGG 2 cut(s) 239, 365
PspGI CCWGG 2 cut(s) 239, 365
PspN4I GGNNCC 5 cut(s) 38, 131, 146, 161, 573
PstI CTGCAG 2 cut(s) 45, 189
PsuI RGATCY 1 cut(s) 234
RsaI GTAC 1 cut(s) 215
RsaNI GTAC 1 cut(s) 214
SapI GCTCTTC 1 cut(s) 218
SaqAI TTAA 1 cut(s) 437
SatI GCNGC 2 cut(s) 185, 586
Sau3AI GATC 2 cut(s) 234, 633
SchI GAGTC 1 cut(s) 88
ScrFI CCNGG 2 cut(s) 241, 367
SfaNI GCATC 2 cut(s) 60, 471
SfcI CTRYAG 2 cut(s) 41, 185
SmlI CTYRAG 1 cut(s) 436
SmoI CTYRAG 1 cut(s) 436
Sse9I AATT 4 cut(s) 14, 336, 503, 512
SsiI CCGC 1 cut(s) 73
SspMI CTAG 2 cut(s) 232, 314
StyD4I CCNGG 2 cut(s) 239, 365
TaaI ACNGT 2 cut(s) 305, 455
TaiI ACGT 1 cut(s) 89
TaqII GACCGA 1 cut(s) 418
TasI AATT 4 cut(s) 14, 336, 503, 512
TatI WGTACW 1 cut(s) 213
Tru1I TTAA 1 cut(s) 437
Tru9I TTAA 1 cut(s) 437
TseFI GTSAC 1 cut(s) 358
TseI GCWGC 2 cut(s) 184, 585
Tsp45I GTSAC 1 cut(s) 358
TspDTI ATGAA 5 cut(s) 80, 128, 330, 409, 580
Vha464I CTTAAG 1 cut(s) 436
XagI CCTNNNNNAGG 1 cut(s) 31
XapI RAATTY 1 cut(s) 14
XmiI GTMKAC 1 cut(s) 432
XspI CTAG 2 cut(s) 232, 314
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.