MD06G1026500.v1.1

component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0008374
Physical Location & Seq
Forward (+)
3267375 .. 3272705
5331 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1026500.v1.1.491

Sequence Viewer

Length: 684 bp
ATGGAGGACTGGGAAGAGGAGAAAGTTCCAGCTCTCCTTGCTAAGGACAAACCGATACATAAATGGGATGACGAAGATGTGGATGAAAATGATATTACGGAGTCATGGGAGGATTTAGATGAACCTGCTCCTCTGGAACCTGCAGCAAAGCCTGTTGCTGAGAAGGCACCTAAGAAACCTGCAACAAAACCTGCTGCAAAAGCTACAGAAAAGAAAGGAAAAACCGTTGAAGTAGAAAAGGAAGAGCCACTGGATCCTGTGGCTGAGAAACTTCGCCAACAAAGGCTGGTGGAAGAAGCAGATTATAAGTCCACTAAAGAATTGTTTTCCAGTGGAGGCAATGAAAAAAACATTGACAATTTCATTCCGAAATCCGAAAGTGACTTCCTGGAATATGCAGAACTTATTTCACATAAACTTCGTCCATTTGAGAAAAGTTTCCATTATATTGGTCTACTCAAGGCAGTAATGAGACTGTCAATGACTTCGTTGAAAGGAGCAGATGCAAAGGAAGTTGCATCTTCCATTACAGCAATTGCAAATGAGAAGATAAAAGCAGAGAAGGAAGCAAATGCTGGCAAAAAGAAGACAGGTGCCAAGAAAAAGCAGCTAATTGTTGATAAGGCGGATGATGATATAGCTGTTGAGGGCTATGATCAACTCGATGATTATGATTTTATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
eIF3_subunit

Protein Analysis

228

Amino Acids

25.48

Weight (kDa)

4.87

Isoelectric Point (pI)

38.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
eIF3_subunit PF08597 3 - 227 7.5e-51 Translation initiation factor eIF3 subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 306
Acc36I ACCTGC 4 cut(s) 133, 148, 187, 199
AccB1I GGYRCC 2 cut(s) 166, 593
AccI GTMKAC 1 cut(s) 454
AciI CCGC 1 cut(s) 626
AclWI GGATC 2 cut(s) 248, 261
AfiI CCNNNNNNNGG 1 cut(s) 43
AgsI TTSAA 2 cut(s) 230, 493
AjnI CCWGG 1 cut(s) 387
AluBI AGCT 4 cut(s) 32, 203, 610, 641
AluI AGCT 4 cut(s) 32, 203, 610, 641
Alw26I GTCTC 1 cut(s) 466
AlwI GGATC 2 cut(s) 248, 261
ApeKI GCWGC 3 cut(s) 143, 194, 607
Asp700I GAANNNNTTC 1 cut(s) 437
BamHI GGATCC 1 cut(s) 253
BanI GGYRCC 2 cut(s) 166, 593
BbsI GAAGAC 1 cut(s) 593
BbvI GCAGC 3 cut(s) 155, 181, 619
BciT130I CCWGG 1 cut(s) 389
BclI TGATCA 1 cut(s) 655
BcoDI GTCTC 1 cut(s) 466
BfmI CTRYAG 2 cut(s) 141, 204
BfuAI ACCTGC 4 cut(s) 133, 148, 187, 199
BisI GCNGC 3 cut(s) 144, 195, 608
BlsI GCNGC 3 cut(s) 145, 196, 609
Bme1390I CCNGG 1 cut(s) 389
BmiI GGNNCC 4 cut(s) 138, 168, 255, 595
BmrFI CCNGG 1 cut(s) 389
BmrI ACTGGG 1 cut(s) 19
BmsI GCATC 2 cut(s) 493, 527
BmuI ACTGGG 1 cut(s) 19
BpiI GAAGAC 1 cut(s) 593
Bpu10I CCTNAGC 1 cut(s) 42
BpuEI CTTGAG 1 cut(s) 443
Bsc4I CCNNNNNNNGG 1 cut(s) 43
Bse1I ACTGG 3 cut(s) 14, 255, 330
Bse3DI GCAATG 1 cut(s) 346
BseBI CCWGG 1 cut(s) 389
BseGI GGATG 3 cut(s) 73, 88, 634
BseLI CCNNNNNNNGG 1 cut(s) 43
BseMI GCAATG 1 cut(s) 346
BseMII CTCAG 2 cut(s) 150, 255
BseNI ACTGG 3 cut(s) 14, 255, 330
BseRI GAGGAG 2 cut(s) 32, 120
BseXI GCAGC 3 cut(s) 155, 181, 619
BshNI GGYRCC 2 cut(s) 166, 593
BslI CCNNNNNNNGG 1 cut(s) 43
BsmAI GTCTC 1 cut(s) 466
Bsp143I GATC 2 cut(s) 253, 655
BspACI CCGC 1 cut(s) 626
BspCNI CTCAG 2 cut(s) 151, 256
BspLI GGNNCC 4 cut(s) 138, 168, 255, 595
BspMAI CTGCAG 1 cut(s) 145
BspMI ACCTGC 4 cut(s) 133, 148, 187, 199
BspPI GGATC 2 cut(s) 248, 261
BspQI GCTCTTC 1 cut(s) 237
BspT107I GGYRCC 2 cut(s) 166, 593
BsrDI GCAATG 1 cut(s) 346
BsrI ACTGG 3 cut(s) 14, 255, 330
BssMI GATC 2 cut(s) 253, 655
Bst2UI CCWGG 1 cut(s) 389
Bst4CI ACNGT 2 cut(s) 226, 477
Bst6I CTCTTC 2 cut(s) 9, 237
BstC8I GCNNGC 1 cut(s) 577
BstDEI CTNAG 4 cut(s) 42, 159, 171, 264
BstENI CCTNNNNNAGG 1 cut(s) 41
BstF5I GGATG 3 cut(s) 73, 88, 634
BstKTI GATC 2 cut(s) 256, 658
BstMAI GTCTC 1 cut(s) 466
BstMBI GATC 2 cut(s) 253, 655
BstMWI GCNNNNNNNGC 3 cut(s) 38, 164, 200
BstNI CCWGG 1 cut(s) 389
BstSCI CCNGG 1 cut(s) 387
BstSFI CTRYAG 2 cut(s) 141, 204
BstV1I GCAGC 3 cut(s) 155, 181, 619
BstV2I GAAGAC 1 cut(s) 593
BstX2I RGATCY 1 cut(s) 253
BstXI CCANNNNNNTGG 1 cut(s) 449
BstYI RGATCY 1 cut(s) 253
BtsCI GGATG 3 cut(s) 73, 88, 634
BtsIMutI CAGTG 2 cut(s) 248, 337
BveI ACCTGC 4 cut(s) 133, 148, 187, 199
Cac8I GCNNGC 1 cut(s) 577
CviAII CATG 1 cut(s) 105
CviJI RGCY 9 cut(s) 32, 151, 203, 247, 263, 286, 610, 641, 651
CviKI_1 RGCY 9 cut(s) 32, 151, 203, 247, 263, 286, 610, 641, 651
DdeI CTNAG 4 cut(s) 42, 159, 171, 264
DpnI GATC 2 cut(s) 255, 657
DpnII GATC 2 cut(s) 253, 655
Eam1104I CTCTTC 2 cut(s) 9, 237
EarI CTCTTC 2 cut(s) 9, 237
EciI GGCGGA 1 cut(s) 641
EcoNI CCTNNNNNAGG 1 cut(s) 41
EcoRII CCWGG 1 cut(s) 387
FaeI CATG 1 cut(s) 108
FatI CATG 1 cut(s) 104
FbaI TGATCA 1 cut(s) 655
FblI GTMKAC 1 cut(s) 454
Fnu4HI GCNGC 3 cut(s) 144, 195, 608
FokI GGATG 3 cut(s) 80, 95, 641
Fsp4HI GCNGC 3 cut(s) 144, 195, 608
GluI GCNGC 3 cut(s) 144, 195, 608
Hin1II CATG 1 cut(s) 108
HinfI GANTC 1 cut(s) 101
Hpy166II GTNNAC 2 cut(s) 312, 455
Hpy188I TCNGA 2 cut(s) 369, 376
Hpy188III TCNNGA 1 cut(s) 134
Hpy8I GTNNAC 2 cut(s) 312, 455
HpyAV CCTTC 2 cut(s) 157, 556
HpyCH4III ACNGT 2 cut(s) 226, 477
HpyCH4V TGCA 7 cut(s) 143, 182, 197, 398, 506, 518, 539
HpyF10VI GCNNNNNNNGC 3 cut(s) 38, 164, 200
HpyF3I CTNAG 4 cut(s) 42, 159, 171, 264
Hsp92II CATG 1 cut(s) 108
Ksp22I TGATCA 1 cut(s) 655
Kzo9I GATC 2 cut(s) 253, 655
LguI GCTCTTC 1 cut(s) 237
LmnI GCTCC 2 cut(s) 133, 497
Lsp1109I GCAGC 3 cut(s) 155, 181, 619
LweI GCATC 2 cut(s) 493, 527
MaeIII GTNAC 1 cut(s) 380
MalI GATC 2 cut(s) 255, 657
MboI GATC 2 cut(s) 253, 655
MboII GAAGA 7 cut(s) 26, 86, 254, 305, 513, 559, 598
MfeI CAATTG 1 cut(s) 534
MflI RGATCY 1 cut(s) 253
MluCI AATT 4 cut(s) 320, 358, 534, 612
MlyI GAGTC 1 cut(s) 110
MnlI CCTC 5 cut(s) 10, 103, 141, 329, 640
MroXI GAANNNNTTC 1 cut(s) 437
MspR9I CCNGG 1 cut(s) 389
MunI CAATTG 1 cut(s) 534
MvaI CCWGG 1 cut(s) 389
MwoI GCNNNNNNNGC 3 cut(s) 38, 164, 200
NdeII GATC 2 cut(s) 253, 655
NlaIII CATG 1 cut(s) 108
NlaIV GGNNCC 4 cut(s) 138, 168, 255, 595
NmuCI GTSAC 1 cut(s) 380
PciSI GCTCTTC 1 cut(s) 237
PdmI GAANNNNTTC 1 cut(s) 437
PfoI TCCNGGA 1 cut(s) 387
PkrI GCNGC 3 cut(s) 145, 196, 609
PleI GAGTC 1 cut(s) 109
PpsI GAGTC 1 cut(s) 109
PsiI TTATAA 1 cut(s) 306
Psp6I CCWGG 1 cut(s) 387
PspGI CCWGG 1 cut(s) 387
PspN4I GGNNCC 4 cut(s) 138, 168, 255, 595
PstI CTGCAG 1 cut(s) 145
PsuI RGATCY 1 cut(s) 253
SapI GCTCTTC 1 cut(s) 237
SatI GCNGC 3 cut(s) 144, 195, 608
Sau3AI GATC 2 cut(s) 253, 655
SchI GAGTC 1 cut(s) 110
ScrFI CCNGG 1 cut(s) 389
SfaNI GCATC 2 cut(s) 493, 527
SfcI CTRYAG 2 cut(s) 141, 204
SmlI CTYRAG 1 cut(s) 458
SmoI CTYRAG 1 cut(s) 458
Sse9I AATT 4 cut(s) 320, 358, 534, 612
SsiI CCGC 1 cut(s) 626
StyD4I CCNGG 1 cut(s) 387
TaaI ACNGT 2 cut(s) 226, 477
TaqI TCGA 1 cut(s) 663
TasI AATT 4 cut(s) 320, 358, 534, 612
TscAI CASTG 2 cut(s) 255, 337
TseFI GTSAC 1 cut(s) 380
TseI GCWGC 3 cut(s) 143, 194, 607
Tsp45I GTSAC 1 cut(s) 380
TspDTI ATGAA 4 cut(s) 99, 135, 352, 357
TspGWI ACGGA 1 cut(s) 113
TspRI CASTG 2 cut(s) 255, 337
XagI CCTNNNNNAGG 1 cut(s) 41
XmiI GTMKAC 1 cut(s) 454
XmnI GAANNNNTTC 1 cut(s) 437
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.