Rmu_sc0028304.1_g000002

component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0028304.1
OG0008374
Physical Location & Seq
Forward (+)
1188 .. 3701
2514 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0028304.1_g000002.1.cds

Sequence Viewer

Length: 675 bp
atggaggactgggaagaagatgcgagaattccatccctcctttctaaggagcctgcagtaaaaggtaactgggatgatgaagatgcggatgagaatgacgtgaaggactcatgggaggatgatgatgaacctgctcctccggaacctgcaccaaaggctcctgctgaaaaggctcccaagaaacctgcaacaaaggctgcagaaaagaaaggaaaagctgttgaagtacaaaaggaagagccactagatcccctggctgagaaacttcgccaacaaagactggtggaagaagcagattataaggccactaaagaactgttttctactagagatgagaagaacattgataatttcattcccaaatccgaaagtgacttcctggaatatgccgaacttatttcacataaacttcgtccatttgagaaaagttttcattatatcggtctacttaaggcagtaatgagactgtcaatgacttctttgaaaggagctgatgcaaaagatgttgcttcttcaattacggcaattgcaaatgagaagataaaagctgagaaggaagccaatgctggtaaaaagaagacaggtgccaagaaaaagcagcttcatgttgataagccggatgatgatatagctgttgatggctatgatccccttgatgactatgactttatgtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
eIF3_subunit

Protein Analysis

224

Amino Acids

25.16

Weight (kDa)

4.8

Isoelectric Point (pI)

36.52

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 300
Acc36I ACCTGC 3 cut(s) 139, 154, 193
AccB1I GGYRCC 1 cut(s) 584
AccI GTMKAC 1 cut(s) 445
AccIII TCCGGA 1 cut(s) 139
AciI CCGC 1 cut(s) 86
AclWI GGATC 2 cut(s) 242, 641
AcsI RAATTY 1 cut(s) 27
AfaI GTAC 1 cut(s) 228
AfiI CCNNNNNNNGG 1 cut(s) 46
AflII CTTAAG 1 cut(s) 449
AgsI TTSAA 3 cut(s) 224, 484, 516
AjiI CACGTC 1 cut(s) 100
AjnI CCWGG 2 cut(s) 252, 378
AluBI AGCT 5 cut(s) 218, 491, 548, 601, 632
AluI AGCT 5 cut(s) 218, 491, 548, 601, 632
Alw26I GTCTC 1 cut(s) 457
AlwI GGATC 2 cut(s) 242, 641
Aor13HI TCCGGA 1 cut(s) 139
AoxI GGCC 1 cut(s) 303
ApeKI GCWGC 2 cut(s) 197, 598
ApoI RAATTY 1 cut(s) 27
BanI GGYRCC 1 cut(s) 584
BbsI GAAGAC 1 cut(s) 584
BbvI GCAGC 2 cut(s) 184, 610
BccI CCATC 2 cut(s) 40, 632
BceAI ACGGC 1 cut(s) 537
BciT130I CCWGG 2 cut(s) 254, 380
BcoDI GTCTC 1 cut(s) 457
BfaI CTAG 2 cut(s) 245, 327
BfmI CTRYAG 2 cut(s) 54, 198
BfrI CTTAAG 1 cut(s) 449
BfuAI ACCTGC 3 cut(s) 139, 154, 193
BisI GCNGC 2 cut(s) 198, 599
BlsI GCNGC 2 cut(s) 199, 600
Bme1390I CCNGG 2 cut(s) 254, 380
BmgBI CACGTC 1 cut(s) 100
BmiI GGNNCC 5 cut(s) 51, 144, 159, 174, 586
BmrFI CCNGG 2 cut(s) 254, 380
BmrI ACTGGG 2 cut(s) 19, 79
BmsI GCATC 3 cut(s) 10, 73, 484
BmuI ACTGGG 2 cut(s) 19, 79
BpiI GAAGAC 1 cut(s) 584
BsaJI CCNNGG 1 cut(s) 252
BsaWI WCCGGW 1 cut(s) 139
Bsc4I CCNNNNNNNGG 1 cut(s) 46
Bse1I ACTGG 3 cut(s) 14, 74, 285
BseAI TCCGGA 1 cut(s) 139
BseBI CCWGG 2 cut(s) 254, 380
BseDI CCNNGG 1 cut(s) 252
BseGI GGATG 5 cut(s) 32, 79, 94, 124, 625
BseLI CCNNNNNNNGG 1 cut(s) 46
BseMII CTCAG 2 cut(s) 249, 540
BseNI ACTGG 3 cut(s) 14, 74, 285
BseRI GAGGAG 1 cut(s) 126
BseXI GCAGC 2 cut(s) 184, 610
BsgI GTGCAG 1 cut(s) 132
BshFI GGCC 1 cut(s) 305
BshNI GGYRCC 1 cut(s) 584
BsiSI CCGG 2 cut(s) 140, 617
BslI CCNNNNNNNGG 1 cut(s) 46
BsmAI GTCTC 1 cut(s) 457
BsnI GGCC 1 cut(s) 305
Bsp13I TCCGGA 1 cut(s) 139
Bsp143I GATC 2 cut(s) 247, 646
BspACI CCGC 1 cut(s) 86
BspANI GGCC 1 cut(s) 305
BspCNI CTCAG 2 cut(s) 250, 541
BspEI TCCGGA 1 cut(s) 139
BspLI GGNNCC 5 cut(s) 51, 144, 159, 174, 586
BspMAI CTGCAG 2 cut(s) 58, 202
BspMI ACCTGC 3 cut(s) 139, 154, 193
BspPI GGATC 2 cut(s) 242, 641
BspQI GCTCTTC 1 cut(s) 231
BspT107I GGYRCC 1 cut(s) 584
BspTI CTTAAG 1 cut(s) 449
BsrI ACTGG 3 cut(s) 14, 74, 285
BssECI CCNNGG 1 cut(s) 252
BssMI GATC 2 cut(s) 247, 646
Bst2UI CCWGG 2 cut(s) 254, 380
Bst4CI ACNGT 2 cut(s) 318, 468
Bst6I CTCTTC 1 cut(s) 231
BstAFI CTTAAG 1 cut(s) 449
BstC8I GCNNGC 1 cut(s) 54
BstDEI CTNAG 3 cut(s) 45, 258, 549
BstENI CCTNNNNNAGG 1 cut(s) 44
BstF5I GGATG 5 cut(s) 32, 79, 94, 124, 625
BstKTI GATC 2 cut(s) 250, 649
BstMAI GTCTC 1 cut(s) 457
BstMBI GATC 2 cut(s) 247, 646
BstMWI GCNNNNNNNGC 3 cut(s) 155, 170, 194
BstNI CCWGG 2 cut(s) 254, 380
BstSCI CCNGG 2 cut(s) 252, 378
BstSFI CTRYAG 2 cut(s) 54, 198
BstV1I GCAGC 2 cut(s) 184, 610
BstV2I GAAGAC 1 cut(s) 584
BstX2I RGATCY 1 cut(s) 247
BstYI RGATCY 1 cut(s) 247
BsuRI GGCC 1 cut(s) 305
BtrI CACGTC 1 cut(s) 100
BtsCI GGATG 5 cut(s) 32, 79, 94, 124, 625
BveI ACCTGC 3 cut(s) 139, 154, 193
Cac8I GCNNGC 1 cut(s) 54
Csp6I GTAC 1 cut(s) 227
CviAII CATG 2 cut(s) 111, 605
CviQI GTAC 1 cut(s) 227
DdeI CTNAG 3 cut(s) 45, 258, 549
DpnI GATC 2 cut(s) 249, 648
DpnII GATC 2 cut(s) 247, 646
Eam1104I CTCTTC 1 cut(s) 231
EarI CTCTTC 1 cut(s) 231
EcoNI CCTNNNNNAGG 1 cut(s) 44
EcoRI GAATTC 1 cut(s) 27
EcoRII CCWGG 2 cut(s) 252, 378
FaeI CATG 2 cut(s) 114, 608
FatI CATG 2 cut(s) 110, 604
FblI GTMKAC 1 cut(s) 445
Fnu4HI GCNGC 2 cut(s) 198, 599
FokI GGATG 5 cut(s) 19, 86, 101, 131, 632
Fsp4HI GCNGC 2 cut(s) 198, 599
FspBI CTAG 2 cut(s) 245, 327
GluI GCNGC 2 cut(s) 198, 599
HaeIII GGCC 1 cut(s) 305
HapII CCGG 2 cut(s) 140, 617
Hin1II CATG 2 cut(s) 114, 608
HinfI GANTC 1 cut(s) 107
HpaII CCGG 2 cut(s) 140, 617
Hpy166II GTNNAC 1 cut(s) 446
Hpy188I TCNGA 1 cut(s) 367
Hpy188III TCNNGA 1 cut(s) 140
Hpy8I GTNNAC 1 cut(s) 446
HpyAV CCTTC 2 cut(s) 97, 547
HpyCH4III ACNGT 2 cut(s) 318, 468
HpyCH4IV ACGT 1 cut(s) 99
HpyCH4V TGCA 6 cut(s) 56, 149, 188, 200, 497, 530
HpyF10VI GCNNNNNNNGC 3 cut(s) 155, 170, 194
HpyF3I CTNAG 3 cut(s) 45, 258, 549
HpySE526I ACGT 1 cut(s) 99
Hsp92II CATG 2 cut(s) 114, 608
Kpn2I TCCGGA 1 cut(s) 139
Kzo9I GATC 2 cut(s) 247, 646
LguI GCTCTTC 1 cut(s) 231
LmnI GCTCC 5 cut(s) 49, 139, 163, 178, 488
Lsp1109I GCAGC 2 cut(s) 184, 610
LweI GCATC 3 cut(s) 10, 73, 484
MaeI CTAG 2 cut(s) 245, 327
MaeII ACGT 1 cut(s) 99
MaeIII GTNAC 2 cut(s) 65, 371
MalI GATC 2 cut(s) 249, 648
MboI GATC 2 cut(s) 247, 646
MboII GAAGA 9 cut(s) 26, 29, 92, 248, 299, 349, 504, 550, 589
MfeI CAATTG 1 cut(s) 525
MflI RGATCY 1 cut(s) 247
MluCI AATT 4 cut(s) 27, 349, 516, 525
MlyI GAGTC 1 cut(s) 101
MnlI CCTC 3 cut(s) 47, 109, 147
MroI TCCGGA 1 cut(s) 139
MseI TTAA 1 cut(s) 450
MspCI CTTAAG 1 cut(s) 449
MspI CCGG 2 cut(s) 140, 617
MspR9I CCNGG 2 cut(s) 254, 380
MunI CAATTG 1 cut(s) 525
MvaI CCWGG 2 cut(s) 254, 380
MwoI GCNNNNNNNGC 3 cut(s) 155, 170, 194
NdeII GATC 2 cut(s) 247, 646
NlaIII CATG 2 cut(s) 114, 608
NlaIV GGNNCC 5 cut(s) 51, 144, 159, 174, 586
NmuCI GTSAC 1 cut(s) 371
PciSI GCTCTTC 1 cut(s) 231
PfoI TCCNGGA 1 cut(s) 378
PkrI GCNGC 2 cut(s) 199, 600
PleI GAGTC 1 cut(s) 101
PpsI GAGTC 1 cut(s) 101
PsiI TTATAA 1 cut(s) 300
Psp6I CCWGG 2 cut(s) 252, 378
PspGI CCWGG 2 cut(s) 252, 378
PspN4I GGNNCC 5 cut(s) 51, 144, 159, 174, 586
PstI CTGCAG 2 cut(s) 58, 202
PsuI RGATCY 1 cut(s) 247
RsaI GTAC 1 cut(s) 228
RsaNI GTAC 1 cut(s) 227
SapI GCTCTTC 1 cut(s) 231
SaqAI TTAA 1 cut(s) 450
SatI GCNGC 2 cut(s) 198, 599
Sau3AI GATC 2 cut(s) 247, 646
SchI GAGTC 1 cut(s) 101
ScrFI CCNGG 2 cut(s) 254, 380
SfaNI GCATC 3 cut(s) 10, 73, 484
SfcI CTRYAG 2 cut(s) 54, 198
SmlI CTYRAG 1 cut(s) 449
SmoI CTYRAG 1 cut(s) 449
Sse9I AATT 4 cut(s) 27, 349, 516, 525
SsiI CCGC 1 cut(s) 86
SspMI CTAG 2 cut(s) 245, 327
StyD4I CCNGG 2 cut(s) 252, 378
TaaI ACNGT 2 cut(s) 318, 468
TaiI ACGT 1 cut(s) 102
TaqII GACCGA 1 cut(s) 431
TasI AATT 4 cut(s) 27, 349, 516, 525
TatI WGTACW 1 cut(s) 226
Tru1I TTAA 1 cut(s) 450
Tru9I TTAA 1 cut(s) 450
TseFI GTSAC 1 cut(s) 371
TseI GCWGC 2 cut(s) 197, 598
Tsp45I GTSAC 1 cut(s) 371
TspDTI ATGAA 5 cut(s) 93, 141, 343, 422, 593
Vha464I CTTAAG 1 cut(s) 449
XagI CCTNNNNNAGG 1 cut(s) 44
XapI RAATTY 1 cut(s) 27
XmiI GTMKAC 1 cut(s) 445
XspI CTAG 2 cut(s) 245, 327
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.