Rorug07G0129800

component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0008374
Physical Location & Seq
Forward (+)
10280868 .. 10285038
4171 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0129800.1

Sequence Viewer

Length: 2517 bp
ATGGCTGCTGTGGCTGTGAGAGTGAGACCACAAGGCTCGTGTTTATCGGAGCCATTACACTGCTGCTACAAGTTCAGAGGTGGTGCTACAACGACGCCTTGCTCAGTTTCTTTTGCTTCAAAGTTTCAATCTTTATGTTGCAAATCACAGTTGCTGGGGAAATCGAATTCCAGAGGTGGGTTTTCCGGGATTGATGTTGTTTCGGGCGGTGAACTGATCAAGAGGTACGTTGAGAATGGGAATTGCGAGGATGCGATAGTGGTTTATGTTGAAATGCTTCAACTTGGTTTGCCTGTAGCTGAGGAATTTCACTTCTTTCCGATTTTGATTAAGGCCTTTGGGGGGTTCTGTGATGCGGGCAAGGCTAGGGAGATTCATGGGCATGTGTTGAAATTGGGGGTTTTGGGCGATGTTTACGATGCCAATTCGATTTTGGGGGTTTATTGGAAATGTGGGGAGGTTGAGGATGCGGTTCAGATGTTTGAGAAAATGCCCGAGAGGGATTTGGTGTCCTGGAATACAATGATATCTGGGTTTTGCCATTCACGGGATTATGTGAGCTCGTTGGGGATGTTTAGTCGGATGGTTCGGGGGGATCATTGGGTGTTGCCTAATCGGGTGGCTTCCCTTTCGGCTCTCACTTCGTGCTCTTCCCTTCACTCTTTGGTTCATGGCAGAGAGATTCATGGCTTTGCTCTGAAGCGCTGGCTGCATGCTGATCAGTTTATGGTTAGTGGGTTGATTGATATGTACATGAAATGTGGGGATGTGAGGAATGCGGAATGTGTTTATAGGAGCGTTATTGGTGAAGCCTGTATTAGAGGAAATACAGTGATATGGAATGTGATGATTTCGGGCTATGTCTCTAATGGGTGTTGGCCGCAGGCGGTGGAGTTGTTTCTACAGATGTTGGCCATTGGGTTGTTACCCGATACTTCCACAATGGTGGCCATTACAGGTTTGTGCTCTCAGCTAATGGATTTAGCATTCGGAAAGCAGATCCATAAATTCTGTTTGAGCACTCAATTGAACATCACTGATGTACGAGTTGAAACAGCTCTTATGGACATGTATTTCAAATGTGGTGATAGTACAACTGGTCTTGAAATCTTCAGAAGGTCCCTCAATCGTAACATGGTCATGTGGGGCGCCGCCATCTCAAATTTTGCTCAAAGTAGTCATCCTCGTGAGGCATTGAATCTGTTTCATGACTATATTTTAGAATATGGCTTTGTGGATTCTGTCATAATCTTGGCTGTTCTGCGGGCTTGTTCATCCCTGGCTGTTAAGTCTATAGGGATGGAAATTCATGGGCTACTAGTGAAATTGGGGTTTGATTCAGACCATTTTGTTGCCAGTGCATTAGTTGATTTTTATGCAAAAACCAGAGATATTGAGTCTGCTCAAAAAGTATTTCACAGACTACCAGCTAGAGATTTGGTGTCATGGAATGCCTTGATATCCGGATATACTCAGAATGAGTATCTAGATGAAGCTTTAAAGGCATTCCTTGACATGCAACTTGAAGAAATTAGACCTAATACTGTGACAGTTGCTAGTATTCTGTCAGTTTGTGCTCAGTTGTCAATCATGATGTTGTGTAAGGAGGTTCACGGTTACCTATTACGGCAAGAATTTGAGTCCAATGTTCTTGTGAGCAATTCCCTGATGACTACCTATGCAAAATGTGGAGACATAAAGAGCTCAAGGAGTGTGTTTGTGAAAATGCCTGAAAGAAATTTAGTTTCATGGAACTCAATTCTTCTGGGGTTAGGAATGCATGGCCATGCAGATGAAACATTTGCTTTATTTGAAAAAATGGAAGCGGCAGGATTGGAGCCTGATCATGCAACTTTTACAGCTCTTCTTTCTGCTTGCAGCCATGCAGGGAGGATAGAAGAGGGGCTGAAGTATTTTCAGTGTATGGTAGAAGTTTACAAAATTGAGCCTCAACTTGAACAGTACACCTGCATGGTTGATCTTTTAGGCCGAGCTGGCCATCTAAAGCAAGCATATGATATTATTTCAACAATGCCTTGTGCCTCAGATGATCGTATATGGGGATCATTGCTTGGATCATGCAAAATTCATGGTGATGAAAGACTGGCAGAAGTTGTGGCTGATCATATCTTTAAACTGGATCCTACTAATATTGGCTACCGTACACTTCTTGCAAATTTGTACGAAGATTATGGGAAATGGAATGAAGTTACCAGGGTTAGATCAGACATCAAAGGTGTGGGACTCAAGAAAACGCCTGGGTGTAGTTGGATTGAAGTTGATAACAATCTCCATACCTTTACTGCGGGTGATCATTCTCATAATGAGTCAGAGGAGATATATTTTACAATAGAAAATCTGACAACTGAAATTAGGAAGGCAGGATATATCCCTCAATTACTGACTGTGGGTGAGGGGCTTGATGAGGTTCTTGATGAAGATCTTACATCATCTAGGGACAAGGGTTTGTTGTTTCTGGTTGCAAGTACAAACAATCACCAACAAGTTTCAATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
eIF3_subunit

Protein Analysis

838

Amino Acids

93.14

Weight (kDa)

5.7

Isoelectric Point (pI)

39.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 276 - 322 2.1e-07 PPR repeat family
PPR PF01535 278 - 307 1e-06 PPR repeat
PPR_2 PF13041 479 - 526 1.2e-09 PPR repeat family
PPR PF01535 481 - 508 4.2e-06 PPR repeat
PPR_2 PF13041 580 - 627 1.3e-10 PPR repeat family
PPR_long PF17177 596 - 678 1.9e-06 Pentacotripeptide-repeat region of PRORP
E_motif PF20431 697 - 759 2.1e-15 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1976
Acc36I ACCTGC 1 cut(s) 1976
AccB1I GGYRCC 1 cut(s) 1148
AccIII TCCGGA 1 cut(s) 1463
AclWI GGATC 6 cut(s) 603, 994, 2071, 2083, 2135, 2148
AcoI YGGCCR 5 cut(s) 878, 912, 948, 1783, 1996
AcsI RAATTY 9 cut(s) 166, 305, 1007, 1162, 1305, 1634, 1738, 2085, 2176
AcuI CTGAAG 3 cut(s) 719, 1096, 1928
AcyI GRCGYC 2 cut(s) 95, 1149
AdeI CACNNNGTG 1 cut(s) 645
AfaI GTAC 8 cut(s) 227, 752, 1044, 1093, 1964, 2164, 2183, 2488
AfeI AGCGCT 1 cut(s) 704
AfiI CCNNNNNNNGG 3 cut(s) 177, 342, 547
AflIII ACRYGT 1 cut(s) 1068
AhlI ACTAGT 1 cut(s) 1318
AjnI CCWGG 4 cut(s) 512, 1278, 2213, 2257
AleI CACNNNNGTG 1 cut(s) 944
AluBI AGCT 9 cut(s) 299, 561, 973, 1058, 1430, 1496, 1704, 1862, 1994
AluI AGCT 9 cut(s) 299, 561, 973, 1058, 1430, 1496, 1704, 1862, 1994
Alw21I GWGCWC 6 cut(s) 563, 650, 968, 1022, 1579, 1706
Alw26I GTCTC 3 cut(s) 19, 868, 1686
AlwI GGATC 6 cut(s) 603, 994, 2071, 2083, 2135, 2148
AlwNI CAGNNNCTG 1 cut(s) 154
Ama87I CYCGRG 1 cut(s) 494
Aor13HI TCCGGA 1 cut(s) 1463
Aor51HI AGCGCT 1 cut(s) 704
AoxI GGCC 7 cut(s) 333, 878, 912, 948, 1783, 1987, 1996
ApeKI GCWGC 4 cut(s) 5, 63, 709, 1878
ApoI RAATTY 9 cut(s) 166, 305, 1007, 1162, 1305, 1634, 1738, 2085, 2176
Asp700I GAANNNNTTC 1 cut(s) 276
AspLEI GCGC 2 cut(s) 705, 1151
AspS9I GGNCC 1 cut(s) 1119
AsuC2I CCSGG 1 cut(s) 187
AsuHPI GGTGA 7 cut(s) 221, 818, 1097, 2105, 2321, 2423, 2489
AvaI CYCGRG 1 cut(s) 494
AvaII GGWCC 1 cut(s) 1119
BalI TGGCCA 4 cut(s) 914, 950, 1785, 1998
BamHI GGATCC 1 cut(s) 2140
BanI GGYRCC 1 cut(s) 1148
BanII GRGCYC 2 cut(s) 563, 1706
BauI CACGAG 2 cut(s) 37, 1185
Bbv12I GWGCWC 6 cut(s) 563, 650, 968, 1022, 1579, 1706
BbvCI CCTCAGC 1 cut(s) 300
BbvI GCAGC 3 cut(s) 50, 696, 1890
BccI CCATC 4 cut(s) 577, 1163, 1294, 2007
BceAI ACGGC 1 cut(s) 1643
BciT130I CCWGG 4 cut(s) 514, 1280, 2215, 2259
BclI TGATCA 5 cut(s) 216, 718, 1843, 2122, 2311
BcnI CCSGG 1 cut(s) 187
BcoDI GTCTC 3 cut(s) 19, 868, 1686
BcuI ACTAGT 1 cut(s) 1318
BfaI CTAG 6 cut(s) 366, 1319, 1431, 1487, 1557, 2454
BfmI CTRYAG 3 cut(s) 294, 902, 1293
BfoI RGCGCY 2 cut(s) 706, 1152
BfuAI ACCTGC 1 cut(s) 1976
BglI GCCNNNNNGGC 1 cut(s) 1995
BglII AGATCT 1 cut(s) 2440
BisI GCNGC 7 cut(s) 6, 64, 710, 881, 1152, 1827, 1879
BlsI GCNGC 7 cut(s) 7, 65, 711, 882, 1153, 1828, 1880
Bme1390I CCNGG 5 cut(s) 187, 514, 1280, 2215, 2259
Bme18I GGWCC 1 cut(s) 1119
BmeT110I CYCGRG 1 cut(s) 494
BmgT120I GGNCC 1 cut(s) 1119
BmiI GGNNCC 5 cut(s) 51, 1121, 1150, 1839, 2142
BmrFI CCNGG 5 cut(s) 187, 514, 1280, 2215, 2259
BmsI GCATC 4 cut(s) 241, 343, 409, 457
Bpu10I CCTNAGC 1 cut(s) 300
BpuEI CTTGAG 2 cut(s) 1690, 2231
BpuMI CCSGG 1 cut(s) 187
BsaBI GATNNNNATC 2 cut(s) 2286, 2439
BsaHI GRCGYC 2 cut(s) 95, 1149
BsaI GGTCTC 1 cut(s) 19
BsaJI CCNNGG 3 cut(s) 1278, 2214, 2258
BsaWI WCCGGW 1 cut(s) 1463
Bsc4I CCNNNNNNNGG 3 cut(s) 177, 342, 547
Bse1I ACTGG 4 cut(s) 1102, 1356, 2109, 2142
Bse3DI GCAATG 1 cut(s) 2066
Bse8I GATNNNNATC 2 cut(s) 2286, 2439
BseAI TCCGGA 1 cut(s) 1463
BseBI CCWGG 4 cut(s) 514, 1280, 2215, 2259
BseDI CCNNGG 3 cut(s) 1278, 2214, 2258
BseGI GGATG 8 cut(s) 256, 472, 576, 588, 772, 1180, 1274, 1305
BseJI GATNNNNATC 2 cut(s) 2286, 2439
BseLI CCNNNNNNNGG 3 cut(s) 177, 342, 547
BseMI GCAATG 1 cut(s) 2066
BseMII CTCAG 6 cut(s) 117, 291, 983, 1487, 1592, 2058
BseNI ACTGG 4 cut(s) 1102, 1356, 2109, 2142
BseRI GAGGAG 1 cut(s) 2348
BseXI GCAGC 3 cut(s) 50, 696, 1890
BseYI CCCAGC 1 cut(s) 154
BshFI GGCC 7 cut(s) 335, 880, 914, 950, 1785, 1989, 1998
BshNI GGYRCC 1 cut(s) 1148
BsiHKAI GWGCWC 6 cut(s) 563, 650, 968, 1022, 1579, 1706
BsiHKCI CYCGRG 1 cut(s) 494
BsiSI CCGG 2 cut(s) 186, 1464
BslFI GGGAC 3 cut(s) 1105, 2256, 2471
BslI CCNNNNNNNGG 3 cut(s) 177, 342, 547
BsmAI GTCTC 3 cut(s) 19, 868, 1686
BsmFI GGGAC 3 cut(s) 1105, 2256, 2471
BsmI GAATGC 5 cut(s) 781, 986, 1456, 1505, 1782
BsnI GGCC 7 cut(s) 335, 880, 914, 950, 1785, 1989, 1998
Bso31I GGTCTC 1 cut(s) 19
BsoBI CYCGRG 1 cut(s) 494
Bsp1286I GDGCHC 6 cut(s) 563, 650, 968, 1022, 1579, 1706
Bsp13I TCCGGA 1 cut(s) 1463
Bsp1407I TGTACA 1 cut(s) 750
BspANI GGCC 7 cut(s) 335, 880, 914, 950, 1785, 1989, 1998
BspCNI CTCAG 6 cut(s) 116, 292, 982, 1486, 1591, 2057
BspEI TCCGGA 1 cut(s) 1463
BspHI TCATGA 2 cut(s) 1207, 1590
BspLI GGNNCC 5 cut(s) 51, 1121, 1150, 1839, 2142
BspMI ACCTGC 1 cut(s) 1976
BspPI GGATC 6 cut(s) 603, 994, 2071, 2083, 2135, 2148
BspQI GCTCTTC 2 cut(s) 655, 1869
BspT107I GGYRCC 1 cut(s) 1148
BspTNI GGTCTC 1 cut(s) 19
BsrDI GCAATG 1 cut(s) 2066
BsrGI TGTACA 1 cut(s) 750
BsrI ACTGG 4 cut(s) 1102, 1356, 2109, 2142
BssECI CCNNGG 3 cut(s) 1278, 2214, 2258
BssNI GRCGYC 2 cut(s) 95, 1149
BssSI CACGAG 2 cut(s) 37, 1185
Bst2BI CACGAG 2 cut(s) 37, 1185
Bst2UI CCWGG 4 cut(s) 514, 1280, 2215, 2259
Bst4CI ACNGT 8 cut(s) 150, 832, 1546, 1552, 1616, 1962, 2162, 2407
Bst6I CTCTTC 3 cut(s) 655, 1869, 1893
BstACI GRCGYC 2 cut(s) 95, 1149
BstAUI TGTACA 1 cut(s) 750
BstC8I GCNNGC 8 cut(s) 358, 707, 714, 885, 1266, 1876, 1996, 2010
BstDEI CTNAG 6 cut(s) 103, 300, 969, 1473, 1578, 2044
BstEII GGTNACC 1 cut(s) 1616
BstF5I GGATG 8 cut(s) 256, 472, 576, 588, 772, 1180, 1274, 1305
BstH2I RGCGCY 2 cut(s) 706, 1152
BstHHI GCGC 2 cut(s) 705, 1151
BstMAI GTCTC 3 cut(s) 19, 868, 1686
BstMWI GCNNNNNNNGC 5 cut(s) 11, 362, 709, 1502, 1995
BstNI CCWGG 4 cut(s) 514, 1280, 2215, 2259
BstNSI RCATGY 4 cut(s) 386, 716, 1072, 1519
BstPI GGTNACC 1 cut(s) 1616
BstSCI CCNGG 5 cut(s) 185, 512, 1278, 2213, 2257
BstSFI CTRYAG 3 cut(s) 294, 902, 1293
BstV1I GCAGC 3 cut(s) 50, 696, 1890
BstX2I RGATCY 3 cut(s) 999, 2140, 2440
BstXI CCANNNNNNTGG 1 cut(s) 946
BstYI RGATCY 3 cut(s) 999, 2140, 2440
BsuRI GGCC 7 cut(s) 335, 880, 914, 950, 1785, 1989, 1998
BtgZI GCGATG 1 cut(s) 423
BtsCI GGATG 8 cut(s) 256, 472, 576, 588, 772, 1180, 1274, 1305
BtsI GCAGTG 1 cut(s) 58
BtsIMutI CAGTG 5 cut(s) 58, 837, 1035, 1363, 1925
BveI ACCTGC 1 cut(s) 1976
Cac8I GCNNGC 8 cut(s) 358, 707, 714, 885, 1266, 1876, 1996, 2010
CaiI CAGNNNCTG 1 cut(s) 154
CciI TCATGA 2 cut(s) 1207, 1590
CfoI GCGC 2 cut(s) 705, 1151
Cfr13I GGNCC 1 cut(s) 1119
CseI GACGC 1 cut(s) 103
Csp6I GTAC 8 cut(s) 226, 751, 1043, 1092, 1963, 2163, 2182, 2487
CviQI GTAC 8 cut(s) 226, 751, 1043, 1092, 1963, 2163, 2182, 2487
DdeI CTNAG 6 cut(s) 103, 300, 969, 1473, 1578, 2044
DinI GGCGCC 1 cut(s) 1150
DraI TTTAAA 2 cut(s) 1500, 2134
DraIII CACNNNGTG 1 cut(s) 645
EaeI YGGCCR 5 cut(s) 878, 912, 948, 1783, 1996
Eam1104I CTCTTC 3 cut(s) 655, 1869, 1893
EarI CTCTTC 3 cut(s) 655, 1869, 1893
Ecl136II GAGCTC 2 cut(s) 561, 1704
Eco147I AGGCCT 1 cut(s) 335
Eco24I GRGCYC 2 cut(s) 563, 1706
Eco31I GGTCTC 1 cut(s) 19
Eco32I GATATC 2 cut(s) 528, 1461
Eco47I GGWCC 1 cut(s) 1119
Eco47III AGCGCT 1 cut(s) 704
Eco53kI GAGCTC 2 cut(s) 561, 1704
Eco57I CTGAAG 3 cut(s) 719, 1096, 1928
Eco88I CYCGRG 1 cut(s) 494
Eco91I GGTNACC 1 cut(s) 1616
EcoICRI GAGCTC 2 cut(s) 561, 1704
EcoO109I RGGNCCY 1 cut(s) 1119
EcoO65I GGTNACC 1 cut(s) 1616
EcoRI GAATTC 1 cut(s) 166
EcoRII CCWGG 4 cut(s) 512, 1278, 2213, 2257
EcoRV GATATC 2 cut(s) 528, 1461
EcoT22I ATGCAT 1 cut(s) 1782
EcoT38I GRGCYC 2 cut(s) 563, 1706
EgeI GGCGCC 1 cut(s) 1150
EheI GGCGCC 1 cut(s) 1150
FaqI GGGAC 3 cut(s) 1105, 2256, 2471
FauI CCCGC 3 cut(s) 349, 1257, 2299
FauNDI CATATG 1 cut(s) 2014
FbaI TGATCA 5 cut(s) 216, 718, 1843, 2122, 2311
Fnu4HI GCNGC 7 cut(s) 6, 64, 710, 881, 1152, 1827, 1879
FokI GGATG 8 cut(s) 263, 479, 583, 595, 779, 1167, 1261, 1312
FriOI GRGCYC 2 cut(s) 563, 1706
Fsp4HI GCNGC 7 cut(s) 6, 64, 710, 881, 1152, 1827, 1879
FspBI CTAG 6 cut(s) 366, 1319, 1431, 1487, 1557, 2454
GlaI GCGC 2 cut(s) 704, 1150
GluI GCNGC 7 cut(s) 6, 64, 710, 881, 1152, 1827, 1879
GsaI CCCAGC 1 cut(s) 158
HaeII RGCGCY 2 cut(s) 706, 1152
HaeIII GGCC 7 cut(s) 335, 880, 914, 950, 1785, 1989, 1998
HapII CCGG 2 cut(s) 186, 1464
HgaI GACGC 1 cut(s) 103
HhaI GCGC 2 cut(s) 705, 1151
Hin1I GRCGYC 2 cut(s) 95, 1149
Hin6I GCGC 2 cut(s) 703, 1149
HinP1I GCGC 2 cut(s) 703, 1149
HindIII AAGCTT 1 cut(s) 1494
HinfI GANTC 9 cut(s) 373, 682, 1198, 1238, 1337, 1397, 1640, 2244, 2327
HpaII CCGG 2 cut(s) 186, 1464
HphI GGTGA 7 cut(s) 221, 818, 1097, 2105, 2321, 2423, 2489
Hpy166II GTNNAC 6 cut(s) 212, 415, 1612, 1936, 1965, 2165
Hpy8I GTNNAC 6 cut(s) 212, 415, 1612, 1936, 1965, 2165
Hpy99I CGWCG 1 cut(s) 97
HpyAV CCTTC 3 cut(s) 665, 1110, 2371
HpyCH4III ACNGT 8 cut(s) 150, 832, 1546, 1552, 1616, 1962, 2162, 2407
HpyCH4IV ACGT 1 cut(s) 228
HpyF10VI GCNNNNNNNGC 5 cut(s) 11, 362, 709, 1502, 1995
HpyF3I CTNAG 6 cut(s) 103, 300, 969, 1473, 1578, 2044
HpySE526I ACGT 1 cut(s) 228
Hsp92I GRCGYC 2 cut(s) 95, 1149
HspAI GCGC 2 cut(s) 703, 1149
KasI GGCGCC 1 cut(s) 1148
Kpn2I TCCGGA 1 cut(s) 1463
Ksp22I TGATCA 5 cut(s) 216, 718, 1843, 2122, 2311
LguI GCTCTTC 2 cut(s) 655, 1869
LmnI GCTCC 3 cut(s) 49, 795, 1837
Lsp1109I GCAGC 3 cut(s) 50, 696, 1890
LweI GCATC 4 cut(s) 241, 343, 409, 457
MaeI CTAG 6 cut(s) 366, 1319, 1431, 1487, 1557, 2454
MaeII ACGT 1 cut(s) 228
MaeIII GTNAC 5 cut(s) 924, 1130, 1546, 1616, 2209
MboII GAAGA 8 cut(s) 642, 1102, 1538, 1754, 1856, 1910, 2198, 2450
MfeI CAATTG 1 cut(s) 1025
MflI RGATCY 3 cut(s) 999, 2140, 2440
MhlI GDGCHC 6 cut(s) 563, 650, 968, 1022, 1579, 1706
MlsI TGGCCA 4 cut(s) 914, 950, 1785, 1998
MluNI TGGCCA 4 cut(s) 914, 950, 1785, 1998
Mly113I GGCGCC 1 cut(s) 1149
MlyI GAGTC 4 cut(s) 1406, 1649, 2238, 2336
MmeI TCCRAC 2 cut(s) 560, 2249
Mox20I TGGCCA 4 cut(s) 914, 950, 1785, 1998
Mph1103I ATGCAT 1 cut(s) 1782
MroI TCCGGA 1 cut(s) 1463
MroXI GAANNNNTTC 1 cut(s) 276
MscI TGGCCA 4 cut(s) 914, 950, 1785, 1998
MseI TTAA 4 cut(s) 330, 1287, 1499, 2133
MslI CAYNNNNRTG 8 cut(s) 381, 944, 1139, 1185, 1785, 1791, 1970, 2094
Msp20I TGGCCA 4 cut(s) 914, 950, 1785, 1998
MspI CCGG 2 cut(s) 186, 1464
MspR9I CCNGG 5 cut(s) 187, 514, 1280, 2215, 2259
MunI CAATTG 1 cut(s) 1025
Mva1269I GAATGC 5 cut(s) 781, 986, 1456, 1505, 1782
MvaI CCWGG 4 cut(s) 514, 1280, 2215, 2259
MwoI GCNNNNNNNGC 5 cut(s) 11, 362, 709, 1502, 1995
NarI GGCGCC 1 cut(s) 1149
NciI CCSGG 1 cut(s) 187
NdeI CATATG 1 cut(s) 2014
NlaIV GGNNCC 5 cut(s) 51, 1121, 1150, 1839, 2142
NmeAIII GCCGAG 1 cut(s) 2015
NmuCI GTSAC 1 cut(s) 1546
NsiI ATGCAT 1 cut(s) 1782
NspI RCATGY 4 cut(s) 386, 716, 1072, 1519
OliI CACNNNNGTG 1 cut(s) 944
PaeI GCATGC 1 cut(s) 716
PagI TCATGA 2 cut(s) 1207, 1590
PaqCI CACCTGC 1 cut(s) 1976
PceI AGGCCT 1 cut(s) 335
PciI ACATGT 1 cut(s) 1068
PciSI GCTCTTC 2 cut(s) 655, 1869
PctI GAATGC 5 cut(s) 781, 986, 1456, 1505, 1782
PdmI GAANNNNTTC 1 cut(s) 276
PfeI GAWTC 5 cut(s) 373, 682, 1198, 1238, 1337
PfoI TCCNGGA 2 cut(s) 185, 512
PkrI GCNGC 7 cut(s) 7, 65, 711, 882, 1153, 1828, 1880
PleI GAGTC 4 cut(s) 1405, 1648, 2238, 2335
PluTI GGCGCC 1 cut(s) 1152
PpsI GAGTC 4 cut(s) 1405, 1648, 2238, 2335
PpuMI RGGWCCY 1 cut(s) 1119
PscI ACATGT 1 cut(s) 1068
Psp124BI GAGCTC 2 cut(s) 563, 1706
Psp5II RGGWCCY 1 cut(s) 1119
Psp6I CCWGG 4 cut(s) 512, 1278, 2213, 2257
PspEI GGTNACC 1 cut(s) 1616
PspFI CCCAGC 1 cut(s) 154
PspGI CCWGG 4 cut(s) 512, 1278, 2213, 2257
PspN4I GGNNCC 5 cut(s) 51, 1121, 1150, 1839, 2142
PspPI GGNCC 1 cut(s) 1119
PspPPI RGGWCCY 1 cut(s) 1119
PstNI CAGNNNCTG 1 cut(s) 154
PsuI RGATCY 3 cut(s) 999, 2140, 2440
RsaI GTAC 8 cut(s) 227, 752, 1044, 1093, 1964, 2164, 2183, 2488
RsaNI GTAC 8 cut(s) 226, 751, 1043, 1092, 1963, 2163, 2182, 2487
RseI CAYNNNNRTG 8 cut(s) 381, 944, 1139, 1185, 1785, 1791, 1970, 2094
SacI GAGCTC 2 cut(s) 563, 1706
SapI GCTCTTC 2 cut(s) 655, 1869
SaqAI TTAA 4 cut(s) 330, 1287, 1499, 2133
SatI GCNGC 7 cut(s) 6, 64, 710, 881, 1152, 1827, 1879
Sau96I GGNCC 1 cut(s) 1119
SchI GAGTC 4 cut(s) 1406, 1649, 2238, 2336
ScrFI CCNGG 5 cut(s) 187, 514, 1280, 2215, 2259
SduI GDGCHC 6 cut(s) 563, 650, 968, 1022, 1579, 1706
SfaNI GCATC 4 cut(s) 241, 343, 409, 457
SfcI CTRYAG 3 cut(s) 294, 902, 1293
SfiI GGCCNNNNNGGCC 1 cut(s) 1995
SfoI GGCGCC 1 cut(s) 1150
SinI GGWCC 1 cut(s) 1119
SmiMI CAYNNNNRTG 8 cut(s) 381, 944, 1139, 1185, 1785, 1791, 1970, 2094
SmlI CTYRAG 2 cut(s) 1705, 2246
SmoI CTYRAG 2 cut(s) 1705, 2246
SpeI ACTAGT 1 cut(s) 1318
SphI GCATGC 1 cut(s) 716
SseBI AGGCCT 1 cut(s) 335
SspDI GGCGCC 1 cut(s) 1148
SspI AATATT 1 cut(s) 2152
SspMI CTAG 6 cut(s) 366, 1319, 1431, 1487, 1557, 2454
SstI GAGCTC 2 cut(s) 563, 1706
StuI AGGCCT 1 cut(s) 335
StyD4I CCNGG 5 cut(s) 185, 512, 1278, 2213, 2257
TaaI ACNGT 8 cut(s) 150, 832, 1546, 1552, 1616, 1962, 2162, 2407
TaiI ACGT 1 cut(s) 231
TaqI TCGA 2 cut(s) 164, 428
TatI WGTACW 4 cut(s) 750, 1091, 1962, 2486
TauI GCSGC 3 cut(s) 883, 1154, 1829
TfiI GAWTC 5 cut(s) 373, 682, 1198, 1238, 1337
Tru1I TTAA 4 cut(s) 330, 1287, 1499, 2133
Tru9I TTAA 4 cut(s) 330, 1287, 1499, 2133
TscAI CASTG 5 cut(s) 65, 837, 1042, 1363, 1925
TseFI GTSAC 1 cut(s) 1546
TseI GCWGC 4 cut(s) 5, 63, 709, 1878
Tsp45I GTSAC 1 cut(s) 1546
TspRI CASTG 5 cut(s) 65, 837, 1042, 1363, 1925
VpaK11BI GGWCC 1 cut(s) 1119
XapI RAATTY 9 cut(s) 166, 305, 1007, 1162, 1305, 1634, 1738, 2085, 2176
XbaI TCTAGA 1 cut(s) 1486
XceI RCATGY 4 cut(s) 386, 716, 1072, 1519
XcmI CCANNNNNNNNNTGG 1 cut(s) 430
XmnI GAANNNNTTC 1 cut(s) 276
XspI CTAG 6 cut(s) 366, 1319, 1431, 1487, 1557, 2454
Zsp2I ATGCAT 1 cut(s) 1782
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.