MD16G1279500.v1.1

component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0008374
Physical Location & Seq
Reverse (-)
37992203 .. 37997673
5471 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1279500.v1.1.491

Sequence Viewer

Length: 684 bp
ATGGAGGACTGGGAAGATGAGAAAGCCCCAACTCTCCTTTCTAAGGACCAACCAGTACATAAATGGGATGATGAAGATTTGGATGAAAATGATATTACCGAGTCATGGGAGGATTTAGATGAACCTGCTCCTTTGGAACCTGCAACAAAGCCTGTTTCTGAGAAGGCACCTAAGAAACCTGCAACAAAACCTGCTGAAAAAGCTACAGAAAAGAAAGGGAAAACCGTTCAAGTAGAAAAGGAAGAGCCACTGGATCCTGTGTCTGAGAAACTTCGCCAACAAAGGCTGGTGGAAGAAGCAGATTATAAGTCCACTAAAGAATTGTTTTCCAGCGGAGGCAATTATAAAACCCTAGACAATTTCATTCCTAAATCTGAAAGTGACTTCCTGGAATATGCAGAACTAATTTCACATAAACTTCGTCCATTTGAGAAAAGTTTCCATTATATTGGTCTACTCAAGGCAGTAATGAGACTGTCAATGACTTCATTGAAAGGAGCAGATGCAAAAGAAGTTTCATCTTCGATTACAGCAATTGCAAATGAGAAAATAAAAGCAGAGAAGGAAGCAAATGCTGGTAAAAAGAAGACAGGCTCCAAGAAAAAGCAGCTACTTGTTGATAAGCCGAATGATGATATAGCTGTTGATGGCTATGGTCCGCTTGATGACTATGATTTTATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
eIF3_subunit

Protein Analysis

228

Amino Acids

25.57

Weight (kDa)

4.93

Isoelectric Point (pI)

37.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
eIF3_subunit PF08597 2 - 227 1.5e-50 Translation initiation factor eIF3 subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 306, 345
Acc36I ACCTGC 4 cut(s) 133, 148, 187, 199
AccB1I GGYRCC 1 cut(s) 166
AccI GTMKAC 1 cut(s) 454
AciI CCGC 2 cut(s) 333, 659
AclWI GGATC 2 cut(s) 248, 261
AfaI GTAC 1 cut(s) 57
AfiI CCNNNNNNNGG 2 cut(s) 43, 105
AgsI TTSAA 2 cut(s) 230, 493
AjnI CCWGG 1 cut(s) 387
AjuI GAANNNNNNNTTGG 2 cut(s) 22, 54
AluBI AGCT 3 cut(s) 203, 610, 641
AluI AGCT 3 cut(s) 203, 610, 641
Alw26I GTCTC 1 cut(s) 466
AlwI GGATC 2 cut(s) 248, 261
ApeKI GCWGC 1 cut(s) 607
Asp700I GAANNNNTTC 1 cut(s) 437
AspS9I GGNCC 2 cut(s) 46, 656
AvaII GGWCC 2 cut(s) 46, 656
BamHI GGATCC 1 cut(s) 253
BanI GGYRCC 1 cut(s) 166
BbsI GAAGAC 1 cut(s) 593
BbvI GCAGC 1 cut(s) 619
BccI CCATC 1 cut(s) 641
BciT130I CCWGG 1 cut(s) 389
BcoDI GTCTC 1 cut(s) 466
BfaI CTAG 1 cut(s) 353
BfmI CTRYAG 1 cut(s) 204
BfuAI ACCTGC 4 cut(s) 133, 148, 187, 199
BisI GCNGC 1 cut(s) 608
BlsI GCNGC 1 cut(s) 609
Bme1390I CCNGG 1 cut(s) 389
Bme18I GGWCC 2 cut(s) 46, 656
BmgT120I GGNCC 2 cut(s) 46, 656
BmiI GGNNCC 4 cut(s) 138, 168, 255, 595
BmrFI CCNGG 1 cut(s) 389
BmrI ACTGGG 1 cut(s) 19
BmsI GCATC 1 cut(s) 493
BmuI ACTGGG 1 cut(s) 19
BpiI GAAGAC 1 cut(s) 593
BpuEI CTTGAG 1 cut(s) 443
Bsc4I CCNNNNNNNGG 2 cut(s) 43, 105
Bse1I ACTGG 3 cut(s) 14, 53, 255
BseBI CCWGG 1 cut(s) 389
BseGI GGATG 2 cut(s) 73, 88
BseLI CCNNNNNNNGG 2 cut(s) 43, 105
BseMII CTCAG 2 cut(s) 150, 255
BseNI ACTGG 3 cut(s) 14, 53, 255
BseXI GCAGC 1 cut(s) 619
BshNI GGYRCC 1 cut(s) 166
BslI CCNNNNNNNGG 2 cut(s) 43, 105
BsmAI GTCTC 1 cut(s) 466
Bsp143I GATC 1 cut(s) 253
BspACI CCGC 2 cut(s) 333, 659
BspCNI CTCAG 2 cut(s) 151, 256
BspLI GGNNCC 4 cut(s) 138, 168, 255, 595
BspMI ACCTGC 4 cut(s) 133, 148, 187, 199
BspPI GGATC 2 cut(s) 248, 261
BspQI GCTCTTC 1 cut(s) 237
BspT107I GGYRCC 1 cut(s) 166
BsrI ACTGG 3 cut(s) 14, 53, 255
BssMI GATC 1 cut(s) 253
Bst2UI CCWGG 1 cut(s) 389
Bst4CI ACNGT 2 cut(s) 226, 477
Bst6I CTCTTC 1 cut(s) 237
BstDEI CTNAG 4 cut(s) 42, 159, 171, 264
BstENI CCTNNNNNAGG 1 cut(s) 41
BstF5I GGATG 2 cut(s) 73, 88
BstKTI GATC 1 cut(s) 256
BstMAI GTCTC 1 cut(s) 466
BstMBI GATC 1 cut(s) 253
BstMWI GCNNNNNNNGC 1 cut(s) 200
BstNI CCWGG 1 cut(s) 389
BstSCI CCNGG 1 cut(s) 387
BstSFI CTRYAG 1 cut(s) 204
BstV1I GCAGC 1 cut(s) 619
BstV2I GAAGAC 1 cut(s) 593
BstX2I RGATCY 1 cut(s) 253
BstXI CCANNNNNNTGG 1 cut(s) 449
BstYI RGATCY 1 cut(s) 253
BtsCI GGATG 2 cut(s) 73, 88
BtsIMutI CAGTG 1 cut(s) 248
BveI ACCTGC 4 cut(s) 133, 148, 187, 199
Cfr13I GGNCC 2 cut(s) 46, 656
Csp6I GTAC 1 cut(s) 56
CviAII CATG 1 cut(s) 105
CviQI GTAC 1 cut(s) 56
DdeI CTNAG 4 cut(s) 42, 159, 171, 264
DpnI GATC 1 cut(s) 255
DpnII GATC 1 cut(s) 253
Eam1104I CTCTTC 1 cut(s) 237
EarI CTCTTC 1 cut(s) 237
Eco47I GGWCC 2 cut(s) 46, 656
EcoNI CCTNNNNNAGG 1 cut(s) 41
EcoRII CCWGG 1 cut(s) 387
FaeI CATG 1 cut(s) 108
FatI CATG 1 cut(s) 104
FblI GTMKAC 1 cut(s) 454
Fnu4HI GCNGC 1 cut(s) 608
FokI GGATG 2 cut(s) 80, 95
Fsp4HI GCNGC 1 cut(s) 608
FspBI CTAG 1 cut(s) 353
GluI GCNGC 1 cut(s) 608
Hin1II CATG 1 cut(s) 108
HinfI GANTC 1 cut(s) 101
Hpy166II GTNNAC 2 cut(s) 312, 455
Hpy188I TCNGA 3 cut(s) 160, 265, 376
Hpy8I GTNNAC 2 cut(s) 312, 455
HpyAV CCTTC 2 cut(s) 157, 556
HpyCH4III ACNGT 2 cut(s) 226, 477
HpyCH4V TGCA 5 cut(s) 143, 182, 398, 506, 539
HpyF10VI GCNNNNNNNGC 1 cut(s) 200
HpyF3I CTNAG 4 cut(s) 42, 159, 171, 264
Hsp92II CATG 1 cut(s) 108
Kzo9I GATC 1 cut(s) 253
LguI GCTCTTC 1 cut(s) 237
LmnI GCTCC 3 cut(s) 133, 497, 599
Lsp1109I GCAGC 1 cut(s) 619
LweI GCATC 1 cut(s) 493
MaeI CTAG 1 cut(s) 353
MaeIII GTNAC 1 cut(s) 380
MalI GATC 1 cut(s) 255
MboI GATC 1 cut(s) 253
MboII GAAGA 6 cut(s) 26, 86, 254, 305, 513, 598
MfeI CAATTG 1 cut(s) 534
MflI RGATCY 1 cut(s) 253
MluCI AATT 5 cut(s) 320, 340, 358, 405, 534
MlyI GAGTC 1 cut(s) 110
MnlI CCTC 2 cut(s) 103, 329
MroXI GAANNNNTTC 1 cut(s) 437
MspA1I CMGCKG 1 cut(s) 333
MspR9I CCNGG 1 cut(s) 389
MunI CAATTG 1 cut(s) 534
MvaI CCWGG 1 cut(s) 389
MwoI GCNNNNNNNGC 1 cut(s) 200
NdeII GATC 1 cut(s) 253
NlaIII CATG 1 cut(s) 108
NlaIV GGNNCC 4 cut(s) 138, 168, 255, 595
NmuCI GTSAC 1 cut(s) 380
PciSI GCTCTTC 1 cut(s) 237
PdmI GAANNNNTTC 1 cut(s) 437
PfoI TCCNGGA 1 cut(s) 387
PkrI GCNGC 1 cut(s) 609
PleI GAGTC 1 cut(s) 109
PpsI GAGTC 1 cut(s) 109
PsiI TTATAA 2 cut(s) 306, 345
Psp6I CCWGG 1 cut(s) 387
PspGI CCWGG 1 cut(s) 387
PspN4I GGNNCC 4 cut(s) 138, 168, 255, 595
PspPI GGNCC 2 cut(s) 46, 656
PsuI RGATCY 1 cut(s) 253
RsaI GTAC 1 cut(s) 57
RsaNI GTAC 1 cut(s) 56
SapI GCTCTTC 1 cut(s) 237
SatI GCNGC 1 cut(s) 608
Sau3AI GATC 1 cut(s) 253
Sau96I GGNCC 2 cut(s) 46, 656
SchI GAGTC 1 cut(s) 110
ScrFI CCNGG 1 cut(s) 389
SetI ASST 8 cut(s) 127, 142, 172, 181, 193, 205, 612, 643
SfaNI GCATC 1 cut(s) 493
SfcI CTRYAG 1 cut(s) 204
SinI GGWCC 2 cut(s) 46, 656
SmlI CTYRAG 1 cut(s) 458
SmoI CTYRAG 1 cut(s) 458
Sse9I AATT 5 cut(s) 320, 340, 358, 405, 534
SsiI CCGC 2 cut(s) 333, 659
SspMI CTAG 1 cut(s) 353
StyD4I CCNGG 1 cut(s) 387
TaaI ACNGT 2 cut(s) 226, 477
TaqI TCGA 1 cut(s) 524
TasI AATT 5 cut(s) 320, 340, 358, 405, 534
TatI WGTACW 1 cut(s) 55
TscAI CASTG 1 cut(s) 255
TseFI GTSAC 1 cut(s) 380
TseI GCWGC 1 cut(s) 607
Tsp45I GTSAC 1 cut(s) 380
TspDTI ATGAA 6 cut(s) 87, 99, 135, 352, 477, 507
TspRI CASTG 1 cut(s) 255
VpaK11BI GGWCC 2 cut(s) 46, 656
XagI CCTNNNNNAGG 1 cut(s) 41
XcmI CCANNNNNNNNNTGG 1 cut(s) 60
XmiI GTMKAC 1 cut(s) 454
XmnI GAANNNNTTC 1 cut(s) 437
XspI CTAG 1 cut(s) 353
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.