Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb5

Orthogroup ID

OG0005765

Physical Location & Seq

Genomic Coordinates Forward (+)

17352664 .. 17366907

Gene Structure 14244 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_5g25870.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3516 bp

ATGCAACAGCAGCAGCACAAGAGGAGGACGAGTGCCGGAGACTTGGCTCACCTCCGTGAGCTGTTCCGGCACCACATCGAGTCTTTCGATTACCTCGTCTCCGCCGGCCTCGGCGTCATGCTCCGTGACATCAAGCCCGTCCAAGTCGACAATCCTCTCACCGGAGAAACCCTTCGCATCTGGTTCGCCGATGATCCTATCCTAGGCCGACCTCTCAAGGACCGGAGCACCGAGCCTCTCTATCCTTTTGAATGTCGCCAGACTCGAATTTCATACACAGGGAAATTGTTGGCAGACATTTATTTCCAGCGTGGGGATAAGGCTCCTGTTAGAGAGCAGTTCAATTTCGGCCAGTTTCCCATCATGCTCATGTCAAAGTGTTGCAACTTAAGAGGTCATGATCCTCAGCAGCTTGTTTATTGCAAAGAAGAGTCATCAGAAATGGGTGGCTACTTCATTTTAAATGGGCTCGAGAGAGTTATTCGACCCGTTATATTGCCAAAGCGGAACTATCCAATGAGTACGGTGCGCAATTCATTTCGTGATCGAAAGGAAGGGTATACAGATAAAGCAGTTGTGATAAGATGTGCAAGAGATGATCAGTCCTCAGTAACAGTCAAATTGTACTACCTGCAAAACGGAAGCGCAAGACTTGGTGTTTGGATACAGGGAAAGGAGTTCTTGCTTCCTGTGGGTCTTATTTTAAAGGCCCTTATTGACACTCCTGATAATGAACTTTATGCGAGTTTGACATGCTGTTATCCTGAGAAATTTGAGAAAGGAAAGGGTGCTGTTGGCACTCAGCTTGTGGGTGAGAGGGCGCAAATCATTATAAATGAAGTAGTGGACTTGGGTCTTTTCACTCGTGGTCAATGTCTAGAACACATTGGTGAGCACTTCCAACCTCTAATGGGTGGACTGGAGAATGAACCTTATTCAGTTGTTGGAGATGCCGTTCTTAAGGACTACATATTTGTTCACCTAGATAAGAATGAAGACAAATTCAATCTCCTCATATTCATGTTGCAGAAACTTTTTTCGCTGATGGATCAGACTTCAGTGCTTGACAACCCAGATTCCCTGCAAAATCAAGAAGTCTTGCTTCCTGGGCACTTGATTACCATATATCTCAAGGAAAAACTAGAGGATTGGTTGCAAAGGACCAAAAAACTTCTTCAAGATGAGATTAAAAAGGAAAGCAACAATATCAATTTTGGCAGCGTCGATAGCTTAAAAAAGGTTCTGGATAAAAATCCTACAAGGCAAATCAGTTTAGCAGTTGAGAATATGTTGAAAACTGGAAGAATTAATACTCAAACAGGTCTAGATTTGCAGCAGAGAGCAGGCTTTTCAGTTCAGGCAGAAAGGCTTAACTTTTTACGTTTTCTATCCCATTTTCGTGCTGTTCATCGAGGGGCTTCATTTGCTGGGCTTCGTACCACAAGTGTTCGGAAATTGCTTCCCGAATCTTGGGGTTTCCTTTGCCCTGTGCACACACCTGATGGAGAGCCATGTGGCTTGTTGAACCATATGACTCGCACTTGTAGAATTACATCGTACTTTGATGCGCAAGGGAATATTAGAGATTTCACTAAGATGCAGATGTCTATTCTCAATGTTCTGATTGAAGTTGGAATGATACCTTCACTGCCAATGTTAGTTCATGCGGGTCTTCCTGCCAATCTTTCAGTTCTTTTAGATGGTCGTGTTGTTGGTTATATATCTTCCACTCAAGTTGAGAAAGTTGTTGCTCATTTAAGGAAGCTGAAAGTGTCAGCTGCTTCAGTGATTCCAGATGACTTGGAAGTGGGATACGTTCCTCTAAGCATGGGTGGGGCATATCCTGGTCTCTACCTTTTCACTTCTCCTTCTAGATTTGTTCGGCCAGTTAAGAATATTTCGATCTCTGCTGTTGAAGGTCAAAACATTGAACTCATTGGGCCCTTTGAACAGGTGTTCATGGAAATAAGATGTCCTGATGGTCGAGATGGTGGAAGGAAAGATGCTTTCCCTGCAACTCATGAAGAAATTCATCCAACTGGGATGCTCAGTGTTGTTGCTAATCTTACTCCCTGGTCAGAACATAATCAGAGTCCACGAAATATGTACCAGTGTCAGATGGCAAAACAAACAATGGGTTTCTCATTACAAGCACTTCGCAATCGTGCAGATCAGAAGTTGTATCATCTGAAGACTCCTCAATCTCCCATTGTCCGCACAAAAACATATACAAATTACTGTGTAGATGAATATCCAACGGGCACAAATGCAATCGTTGCTGTACTGTCATATACTGGGTACGATATGGAGGATGCTATGATTCTAAGCAAGTCATCTGTAGATCGCGGAATGTTTCACGGATTAGTGTATCAGACGGAATCCATTGACTTGTCTGATCAAAAGGGTAGGTCAGGATCCCAGAGATCGTTCAGGAGAAGTGCTGCTGACAAGTCATATCATTCTCTAATCGATTCAGATGGTCTTCCTTATGTGGGTCAGACAATAAAACCAGATGAACCCTATTATAGCACTTATGATGAGGTAAAAGGTGCCATAAGAACGACAAAACGAAAAGGGTCAGAGCCTGCTATTGTTGACTATGTTGCTATTGATGGGAAGAAACAGCTTCAGAAGGTGAACATACGGTTTCGTCACCCTAGAAACCCTATAATTGGTGATAAATTTAGCAGCCGACATGGACAAAAAGGAGTTTGCTCCCAGTTGTGGCCTGACATTGATATGCCATTTTCTGGAGTTACGGGAATGCGCCCAGATCTTATCATCAATCCACATGCATTTCCATCAAGAATGACGATAGCCATGCTTTTGGAGTCGGTTGCTGCAAAGGGTGGTAGCTTACATGGAAAATATGTTGATGCAACACCATTTGCTAATTCAGAGAAGAAAGTTAAGGAAGAAACTAATGGAGAGAAATCCAAAACCCTTGTTGATGAACTTGGTGAAATGTTAAAGGTTAAAGGGTTTAACTACAATGGTGCAGAGGTATTGTACAGTGGCGTCTATGGAACAGAACTCACGTGTGAAATCTTCATTGGTCCTGTTTATTATCAACGATTACGGCATATGGTTTCAGACAAATTTCAGGTTCGTTCTACGGGAATGGTAGATCAAGTCACACGGCAGCCTATAAAAGGACGAAAACGAGGTGGTGGTATACGTTTTGGTGAAATGGAGAGAGACTCCTTGCTTGCACATGGGGCAGCATATTTGTTGCACGATAGACTTCATACATGTTCTGATTATCACATTGCTGATGTATGTTCCCTCTGTGGATCCATCCTAACAACCTCATCAGTCCAGCCACCAGTACGGGTTTGTGAAATCCCTGGCATGCCCCCCATGAGAGCTCCCAAGAAGGTGACTTGCCATGCATGTAAGACGAGTAAAGGGATGGAGACGGTTGCAATGCCTTATGTGTTTAGATACTTAGCTGCTGAGTTAGCAGCTATGAACATAAAACTGAACCTCCAGTTGAGCAATGCAAGCGGAGCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000428 GO:0003674 GO:0003824 GO:0003899 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005736 GO:0005737 GO:0005829 GO:0006139 GO:0006351 GO:0006360 GO:0006725 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009553 GO:0009561 GO:0009987 GO:0010467 GO:0016070 GO:0016740 GO:0016772 GO:0016779 GO:0018130 GO:0019438 GO:0030880 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032774 GO:0032991 GO:0034062 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044452 GO:0044464 GO:0046483 GO:0048229 GO:0048856 GO:0055029 GO:0061695 GO:0070013 GO:0071704 GO:0090304 GO:0097659 GO:0097747 GO:0140098 GO:1901360 GO:1901362 GO:1901576 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko00230 ko00240 ko01100 ko03020 map00230 map00240 map01100 map03020

Pfam Domains

Protein Families

RNA_pol_Rpa2_4 RNA_pol_Rpb2_1 RNA_pol_Rpb2_2 RNA_pol_Rpb2_3 RNA_pol_Rpb2_6 RNA_pol_Rpb2_7

Protein Analysis

1172

Amino Acids

131.4

Weight (kDa)

8.83

Isoelectric Point (pI)

36.46

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RNA_pol_Rpb2_1	PF04563	21 - 401	2.4e-30	RNA polymerase beta subunit
RNA_pol_Rpb2_2	PF04561	194 - 366	2.8e-08	RNA polymerase Rpb2, domain 2
RNA_pol_Rpb2_3	PF04565	454 - 517	1.2e-27	RNA polymerase Rpb2, domain 3
RNA_pol_Rpa2_4	PF06883	571 - 628	1.3e-21	RNA polymerase I, Rpa2 specific domain
RNA_pol_Rpb2_6	PF00562	686 - 1055	1.7e-120	RNA polymerase Rpb2, domain 6
RNA_pol_Rpb2_7	PF04560	1057 - 1164	1.2e-21	RNA polymerase Rpb2, domain 7

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0005765)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G29940
fragaria_vesca	FvH4_5g25870 FvH4_5g25870 FvH4_7g07420 FvH4_7g07420 FvH4_7g07420 FvH4_7g07420 FvH4_7g07420 FvH4_7g07420 FvH4_7g07420
malus_domestica	MD08G1237100.v1.1 MD15G1428900.v1.1
prunus_persica	Prupe.1G574600_v2.0.a1 Prupe.1G574600_v2.0.a1 Prupe.7G107000_v2.0.a1 Prupe.7G184700_v2.0.a1 Prupe.7G223000_v2.0.a1
pyrus_communis	pycom08g20550
rosa_chinensis	RchiOBHm_Chr1g0339571 RchiOBHm_Chr1g0339591
rosa_laevigata	RLG00000029239
rosa_multiflora	Rmu_sc0015580.1_g000004
rosa_roxburghii	Rroxscaffold_4G00313560 Rroxscaffold_4G00313600
rosa_rugosa	Rorug01G0140100.1
rosa_samantha	Rh1AG158100 Rh1CG147600
rosa_wichuraiana	Rw1G013100

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	833
Acc16I	TGCGCA	2 cut(s)	530, 1569
Acc36I	ACCTGC	1 cut(s)	639
AccB1I	GGYRCC	2 cut(s)	69, 2549
AccB7I	CCANNNNNTGG	1 cut(s)	2750
AccI	GTMKAC	3 cut(s)	147, 560, 3175
AccII	CGCG	1 cut(s)	2346
AciI	CCGC	6 cut(s)	102, 505, 1667, 2215, 2346, 3507
AclWI	GGATC	7 cut(s)	188, 395, 1056, 2409, 2422, 3288, 3301
AcoI	YGGCCR	2 cut(s)	349, 1883
AcsI	RAATTY	6 cut(s)	267, 770, 1001, 2028, 2681, 3098
AcuI	CTGAAG	4 cut(s)	1041, 1767, 2210, 2612
AcvI	CACGTG	1 cut(s)	3039
AcyI	GRCGYC	2 cut(s)	114, 3018
AfaI	GTAC	9 cut(s)	523, 626, 1438, 1559, 2108, 2283, 2300, 3011, 3330
AflII	CTTAAG	2 cut(s)	388, 959
AflIII	ACRYGT	2 cut(s)	3038, 3251
AjnI	CCWGG	4 cut(s)	1105, 1843, 2072, 3346
AjuI	GAANNNNNNNTTGG	2 cut(s)	3036, 3068
AleI	CACNNNNGTG	2 cut(s)	54, 888
AloI	GAACNNNNNNTCC	2 cut(s)	939, 971
Alw21I	GWGCWC	4 cut(s)	230, 897, 1494, 3370
Alw26I	GTCTC	5 cut(s)	33, 103, 1853, 3192, 3410
Alw44I	GTGCAC	1 cut(s)	1490
AlwI	GGATC	7 cut(s)	188, 395, 1056, 2409, 2422, 3288, 3301
AlwNI	CAGNNNCTG	1 cut(s)	2585
Ama87I	CYCGRG	1 cut(s)	470
AoxI	GGCC	7 cut(s)	106, 205, 349, 708, 1883, 1940, 2726
ApaI	GGGCCC	1 cut(s)	1944
ApaLI	GTGCAC	1 cut(s)	1490
ApoI	RAATTY	6 cut(s)	267, 770, 1001, 2028, 2681, 3098
ArsI	GACNNNNNNTTYG	4 cut(s)	642, 674, 956, 988
AseI	ATTAAT	1 cut(s)	1308
Asp700I	GAANNNNTTC	2 cut(s)	171, 2028
AspA2I	CCTAGG	1 cut(s)	202
AspLEI	GCGC	5 cut(s)	531, 647, 823, 1570, 2769
AspS9I	GGNCC	6 cut(s)	220, 709, 1161, 1940, 1941, 3056
AvaI	CYCGRG	1 cut(s)	470
AvaII	GGWCC	3 cut(s)	220, 1161, 3056
AvrII	CCTAGG	1 cut(s)	202
BaeGI	GKGCMC	4 cut(s)	1113, 1494, 1944, 2264
BamHI	GGATCC	2 cut(s)	2414, 3293
BanI	GGYRCC	2 cut(s)	69, 2549
BanII	GRGCYC	3 cut(s)	471, 1944, 3370
BarI	GAAGNNNNNNTAC	2 cut(s)	1294, 1326
BauI	CACGAG	1 cut(s)	864
BbrPI	CACGTG	1 cut(s)	3039
BbsI	GAAGAC	4 cut(s)	1002, 1664, 2198, 2474
Bbv12I	GWGCWC	4 cut(s)	230, 897, 1494, 3370
BbvCI	CCTCAGC	1 cut(s)	405
BceAI	ACGGC	3 cut(s)	938, 3095, 3155
BcgI	CGANNNNNNTGC	4 cut(s)	166, 200, 2803, 2837
BciT130I	CCWGG	4 cut(s)	1107, 1845, 2074, 3348
BciVI	GTATCC	2 cut(s)	657, 1805
BclI	TGATCA	2 cut(s)	598, 2395
BcoDI	GTCTC	5 cut(s)	33, 103, 1853, 3192, 3410
BfaI	CTAG	7 cut(s)	203, 878, 983, 1142, 1325, 1872, 2658
BfmI	CTRYAG	1 cut(s)	2337
BfrI	CTTAAG	2 cut(s)	388, 959
BfuAI	ACCTGC	1 cut(s)	639
BfuI	GTATCC	2 cut(s)	657, 1805
BglII	AGATCT	1 cut(s)	2773
BlnI	CCTAGG	1 cut(s)	202
Bme1390I	CCNGG	4 cut(s)	1107, 1845, 2074, 3348
Bme18I	GGWCC	3 cut(s)	220, 1161, 3056
BmeT110I	CYCGRG	1 cut(s)	470
BmgT120I	GGNCC	6 cut(s)	220, 709, 1161, 1940, 1941, 3056
BmiI	GGNNCC	6 cut(s)	71, 324, 1942, 2416, 2551, 3295
BmrFI	CCNGG	4 cut(s)	1107, 1845, 2074, 3348
BmrI	ACTGGG	3 cut(s)	2049, 2304, 2713
BmsI	GCATC	8 cut(s)	186, 940, 1555, 1587, 1993, 2034, 2302, 2866
BmuI	ACTGGG	3 cut(s)	2049, 2304, 2713
BoxI	GACNNNNGTC	1 cut(s)	852
BpiI	GAAGAC	4 cut(s)	1002, 1664, 2198, 2474
BplI	GAGNNNNNCTC	2 cut(s)	3185, 3217
BpmI	CTGGAG	3 cut(s)	941, 2772, 3473
Bpu10I	CCTNAGC	1 cut(s)	405
BpuEI	CTTGAG	3 cut(s)	200, 1115, 1716
Bsa29I	ATCGAT	1 cut(s)	2469
BsaAI	YACGTR	1 cut(s)	3039
BsaBI	GATNNNNATC	2 cut(s)	1251, 2250
BsaHI	GRCGYC	2 cut(s)	114, 3018
BsaI	GGTCTC	1 cut(s)	1853
BsaJI	CCNNGG	5 cut(s)	109, 202, 1106, 2072, 3346
BsaWI	WCCGGW	2 cut(s)	161, 222
BsaXI	ACNNNNNCTCC	8 cut(s)	16, 46, 83, 113, 939, 969, 3471, 3501
Bse118I	RCCGGY	1 cut(s)	104
Bse3DI	GCAATG	3 cut(s)	3267, 3432, 3505
Bse8I	GATNNNNATC	2 cut(s)	1251, 2250
BseBI	CCWGG	4 cut(s)	1107, 1845, 2074, 3348
BseCI	ATCGAT	1 cut(s)	2469
BseDI	CCNNGG	5 cut(s)	109, 202, 1106, 2072, 3346
BseGI	GGATG	5 cut(s)	2032, 2049, 2317, 3297, 3417
BseJI	GATNNNNATC	2 cut(s)	1251, 2250
BseMI	GCAATG	3 cut(s)	3267, 3432, 3505
BseMII	CTCAG	6 cut(s)	419, 621, 756, 815, 2062, 3447
BseRI	GAGGAG	3 cut(s)	37, 1001, 2187
BseSI	GKGCMC	4 cut(s)	1113, 1494, 1944, 2264
BseYI	CCCAGC	1 cut(s)	1427
BsgI	GTGCAG	2 cut(s)	2187, 3018
Bsh1236I	CGCG	1 cut(s)	2346
BshFI	GGCC	7 cut(s)	108, 207, 351, 710, 1885, 1942, 2728
BshNI	GGYRCC	2 cut(s)	69, 2549
BshVI	ATCGAT	1 cut(s)	2469
BsiHKAI	GWGCWC	4 cut(s)	230, 897, 1494, 3370
BsiHKCI	CYCGRG	1 cut(s)	470
BsiSI	CCGG	5 cut(s)	36, 67, 105, 162, 223
BsmAI	GTCTC	5 cut(s)	33, 103, 1853, 3192, 3410
BsmBI	CGTCTC	2 cut(s)	103, 3410
BsmI	GAATGC	1 cut(s)	2769
BsnI	GGCC	7 cut(s)	108, 207, 351, 710, 1885, 1942, 2728
Bso31I	GGTCTC	1 cut(s)	1853
BsoBI	CYCGRG	1 cut(s)	470
Bsp120I	GGGCCC	1 cut(s)	1940
Bsp1286I	GDGCHC	8 cut(s)	230, 471, 897, 1113, 1494, 1944, 2264, 3370
Bsp1407I	TGTACA	1 cut(s)	3009
BspACI	CCGC	6 cut(s)	102, 505, 1667, 2215, 2346, 3507
BspANI	GGCC	7 cut(s)	108, 207, 351, 710, 1885, 1942, 2728
BspCNI	CTCAG	6 cut(s)	418, 620, 757, 814, 2061, 3448
BspDI	ATCGAT	1 cut(s)	2469
BspFNI	CGCG	1 cut(s)	2346
BspHI	TCATGA	2 cut(s)	397, 2020
BspLI	GGNNCC	6 cut(s)	71, 324, 1942, 2416, 2551, 3295
BspMI	ACCTGC	1 cut(s)	639
BspPI	GGATC	7 cut(s)	188, 395, 1056, 2409, 2422, 3288, 3301
BspT107I	GGYRCC	2 cut(s)	69, 2549
BspTI	CTTAAG	2 cut(s)	388, 959
BspTNI	GGTCTC	1 cut(s)	1853
BsrDI	GCAATG	3 cut(s)	3267, 3432, 3505
BsrFI	RCCGGY	1 cut(s)	104
BsrGI	TGTACA	1 cut(s)	3009
BssAI	RCCGGY	1 cut(s)	104
BssECI	CCNNGG	5 cut(s)	109, 202, 1106, 2072, 3346
BssNAI	GTATAC	2 cut(s)	561, 3176
BssNI	GRCGYC	2 cut(s)	114, 3018
BssSI	CACGAG	1 cut(s)	864
BssT1I	CCWWGG	1 cut(s)	202
Bst1107I	GTATAC	2 cut(s)	561, 3176
Bst2BI	CACGAG	1 cut(s)	864
Bst2UI	CCWGG	4 cut(s)	1107, 1845, 2074, 3348
Bst4CI	ACNGT	7 cut(s)	526, 616, 2240, 2286, 2646, 3014, 3421
Bst6I	CTCTTC	1 cut(s)	423
BstACI	GRCGYC	2 cut(s)	114, 3018
BstAFI	CTTAAG	2 cut(s)	388, 959
BstAPI	GCANNNNNTGC	1 cut(s)	2276
BstAUI	TGTACA	1 cut(s)	3009
BstBAI	YACGTR	1 cut(s)	3039
BstC8I	GCNNGC	6 cut(s)	106, 1345, 2586, 3210, 3353, 3505
BstENI	CCTNNNNNAGG	2 cut(s)	201, 3150
BstF5I	GGATG	5 cut(s)	2032, 2049, 2317, 3297, 3417
BstFNI	CGCG	1 cut(s)	2346
BstHHI	GCGC	5 cut(s)	531, 647, 823, 1570, 2769
BstMAI	GTCTC	5 cut(s)	33, 103, 1853, 3192, 3410
BstNI	CCWGG	4 cut(s)	1107, 1845, 2074, 3348
BstNSI	RCATGY	5 cut(s)	756, 2795, 3255, 3355, 3396
BstPAI	GACNNNNGTC	1 cut(s)	852
BstSCI	CCNGG	4 cut(s)	1105, 1843, 2072, 3346
BstSFI	CTRYAG	1 cut(s)	2337
BstSLI	GKGCMC	4 cut(s)	1113, 1494, 1944, 2264
BstUI	CGCG	1 cut(s)	2346
BstV2I	GAAGAC	4 cut(s)	1002, 1664, 2198, 2474
BstX2I	RGATCY	3 cut(s)	2414, 2773, 3293
BstXI	CCANNNNNNTGG	1 cut(s)	2827
BstYI	RGATCY	3 cut(s)	2414, 2773, 3293
BstZ17I	GTATAC	2 cut(s)	561, 3176
Bsu15I	ATCGAT	1 cut(s)	2469
BsuI	GTATCC	2 cut(s)	657, 1805
BsuRI	GGCC	7 cut(s)	108, 207, 351, 710, 1885, 1942, 2728
BsuTUI	ATCGAT	1 cut(s)	2469
BtsCI	GGATG	5 cut(s)	2032, 2049, 2317, 3297, 3417
BtsI	GCAGTG	1 cut(s)	1646
BtsIMutI	CAGTG	6 cut(s)	1065, 1646, 1791, 2056, 2117, 3019
BveI	ACCTGC	1 cut(s)	639
Cac8I	GCNNGC	6 cut(s)	106, 1345, 2586, 3210, 3353, 3505
CaiI	CAGNNNCTG	1 cut(s)	2585
CciI	TCATGA	2 cut(s)	397, 2020
CfoI	GCGC	5 cut(s)	531, 647, 823, 1570, 2769
Cfr10I	RCCGGY	1 cut(s)	104
Cfr13I	GGNCC	6 cut(s)	220, 709, 1161, 1940, 1941, 3056
ClaI	ATCGAT	1 cut(s)	2469
CseI	GACGC	3 cut(s)	103, 1210, 3007
Csp6I	GTAC	9 cut(s)	522, 625, 1437, 1558, 2107, 2282, 2299, 3010, 3329
CspCI	CAANNNNNGTGG	2 cut(s)	1717, 1752
CviQI	GTAC	9 cut(s)	522, 625, 1437, 1558, 2107, 2282, 2299, 3010, 3329
DraI	TTTAAA	2 cut(s)	462, 705
EaeI	YGGCCR	2 cut(s)	349, 1883
Eam1104I	CTCTTC	1 cut(s)	423
EarI	CTCTTC	1 cut(s)	423
EciI	GGCGGA	1 cut(s)	91
Ecl136II	GAGCTC	1 cut(s)	3368
Eco130I	CCWWGG	1 cut(s)	202
Eco24I	GRGCYC	3 cut(s)	471, 1944, 3370
Eco31I	GGTCTC	1 cut(s)	1853
Eco47I	GGWCC	3 cut(s)	220, 1161, 3056
Eco53kI	GAGCTC	1 cut(s)	3368
Eco57I	CTGAAG	4 cut(s)	1041, 1767, 2210, 2612
Eco72I	CACGTG	1 cut(s)	3039
Eco88I	CYCGRG	1 cut(s)	470
EcoICRI	GAGCTC	1 cut(s)	3368
EcoNI	CCTNNNNNAGG	2 cut(s)	201, 3150
EcoO109I	RGGNCCY	2 cut(s)	709, 1941
EcoRII	CCWGG	4 cut(s)	1105, 1843, 2072, 3346
EcoT14I	CCWWGG	1 cut(s)	202
EcoT22I	ATGCAT	2 cut(s)	2797, 3394
EcoT38I	GRGCYC	3 cut(s)	471, 1944, 3370
ErhI	CCWWGG	1 cut(s)	202
Esp3I	CGTCTC	2 cut(s)	103, 3410
FalI	AAGNNNNNCTT	5 cut(s)	665, 697, 1086, 1118, 3496
FauI	CCCGC	1 cut(s)	1660
FauNDI	CATATG	2 cut(s)	1530, 3084
FbaI	TGATCA	2 cut(s)	598, 2395
FblI	GTMKAC	3 cut(s)	147, 560, 3175
FokI	GGATG	5 cut(s)	2019, 2056, 2324, 3284, 3424
FriOI	GRGCYC	3 cut(s)	471, 1944, 3370
FspBI	CTAG	7 cut(s)	203, 878, 983, 1142, 1325, 1872, 2658
FspI	TGCGCA	2 cut(s)	530, 1569
GlaI	GCGC	5 cut(s)	530, 646, 822, 1569, 2768
GsaI	CCCAGC	1 cut(s)	1431
GsuI	CTGGAG	3 cut(s)	941, 2772, 3473
HaeIII	GGCC	7 cut(s)	108, 207, 351, 710, 1885, 1942, 2728
HapII	CCGG	5 cut(s)	36, 67, 105, 162, 223
HgaI	GACGC	3 cut(s)	103, 1210, 3007
HhaI	GCGC	5 cut(s)	531, 647, 823, 1570, 2769
Hin1I	GRCGYC	2 cut(s)	114, 3018
Hin6I	GCGC	5 cut(s)	529, 645, 821, 1568, 2767
HinP1I	GCGC	5 cut(s)	529, 645, 821, 1568, 2767
HincII	GTYRAC	2 cut(s)	148, 2596
HindII	GTYRAC	2 cut(s)	148, 2596
HpaII	CCGG	5 cut(s)	36, 67, 105, 162, 223
Hpy99I	CGWCG	1 cut(s)	1226
HpyAV	CCTTC	8 cut(s)	182, 548, 1653, 1878, 1910, 1989, 2626, 3370
HpyCH4III	ACNGT	7 cut(s)	526, 616, 2240, 2286, 2646, 3014, 3421
HpyCH4IV	ACGT	4 cut(s)	1381, 1815, 3038, 3178
HpySE526I	ACGT	4 cut(s)	1381, 1815, 3038, 3178
Hsp92I	GRCGYC	2 cut(s)	114, 3018
HspAI	GCGC	5 cut(s)	529, 645, 821, 1568, 2767
KroI	GCCGGC	1 cut(s)	104
KroNI	GCCGGC	1 cut(s)	106
Ksp22I	TGATCA	2 cut(s)	598, 2395
LmnI	GCTCC	6 cut(s)	126, 225, 328, 2720, 3373, 3509
LweI	GCATC	8 cut(s)	186, 940, 1555, 1587, 1993, 2034, 2302, 2866
MaeI	CTAG	7 cut(s)	203, 878, 983, 1142, 1325, 1872, 2658
MaeII	ACGT	4 cut(s)	1381, 1815, 3038, 3178
MaeIII	GTNAC	6 cut(s)	125, 610, 2651, 2755, 3133, 3379
MflI	RGATCY	3 cut(s)	2414, 2773, 3293
MhlI	GDGCHC	8 cut(s)	230, 471, 897, 1113, 1494, 1944, 2264, 3370
MlyI	GAGTC	8 cut(s)	89, 256, 440, 1528, 2101, 2188, 2840, 3194
MmeI	TCCRAC	5 cut(s)	925, 925, 1612, 2060, 2279
Mph1103I	ATGCAT	2 cut(s)	2797, 3394
MroNI	GCCGGC	1 cut(s)	104
MroXI	GAANNNNTTC	2 cut(s)	171, 2028
MslI	CAYNNNNRTG	7 cut(s)	54, 368, 888, 1019, 1398, 1595, 2738
MspA1I	CMGCKG	1 cut(s)	1778
MspCI	CTTAAG	2 cut(s)	388, 959
MspI	CCGG	5 cut(s)	36, 67, 105, 162, 223
MspR9I	CCNGG	4 cut(s)	1107, 1845, 2074, 3348
Mva1269I	GAATGC	1 cut(s)	2769
MvaI	CCWGG	4 cut(s)	1107, 1845, 2074, 3348
MvnI	CGCG	1 cut(s)	2346
NaeI	GCCGGC	1 cut(s)	106
NdeI	CATATG	2 cut(s)	1530, 3084
NgoMIV	GCCGGC	1 cut(s)	104
NlaIV	GGNNCC	6 cut(s)	71, 324, 1942, 2416, 2551, 3295
NmeAIII	GCCGAG	1 cut(s)	90
NmuCI	GTSAC	4 cut(s)	125, 2651, 3133, 3379
NsbI	TGCGCA	2 cut(s)	530, 1569
NsiI	ATGCAT	2 cut(s)	2797, 3394
NspI	RCATGY	5 cut(s)	756, 2795, 3255, 3355, 3396
OliI	CACNNNNGTG	2 cut(s)	54, 888
PaeI	GCATGC	1 cut(s)	3355
PaeR7I	CTCGAG	1 cut(s)	470
PagI	TCATGA	2 cut(s)	397, 2020
PciI	ACATGT	1 cut(s)	3251
PcsI	WCGNNNNNNNCGW	3 cut(s)	84, 93, 262
PctI	GAATGC	1 cut(s)	2769
PdiI	GCCGGC	1 cut(s)	106
PdmI	GAANNNNTTC	2 cut(s)	171, 2028
PfeI	GAWTC	6 cut(s)	1076, 1466, 1789, 2320, 2378, 2471
PflMI	CCANNNNNTGG	1 cut(s)	2750
PleI	GAGTC	8 cut(s)	88, 256, 439, 1528, 2100, 2188, 2839, 3194
PmaCI	CACGTG	1 cut(s)	3039
PmlI	CACGTG	1 cut(s)	3039
PpsI	GAGTC	8 cut(s)	88, 256, 439, 1528, 2100, 2188, 2839, 3194
Ppu21I	YACGTR	1 cut(s)	3039
PscI	ACATGT	1 cut(s)	3251
PshAI	GACNNNNGTC	1 cut(s)	852
PshBI	ATTAAT	1 cut(s)	1308
PsiI	TTATAA	1 cut(s)	833
Psp124BI	GAGCTC	1 cut(s)	3370
Psp6I	CCWGG	4 cut(s)	1105, 1843, 2072, 3346
PspCI	CACGTG	1 cut(s)	3039
PspFI	CCCAGC	1 cut(s)	1427
PspGI	CCWGG	4 cut(s)	1105, 1843, 2072, 3346
PspN4I	GGNNCC	6 cut(s)	71, 324, 1942, 2416, 2551, 3295
PspOMI	GGGCCC	1 cut(s)	1940
PspPI	GGNCC	6 cut(s)	220, 709, 1161, 1940, 1941, 3056
PstNI	CAGNNNCTG	1 cut(s)	2585
PsuI	RGATCY	3 cut(s)	2414, 2773, 3293
PvuII	CAGCTG	1 cut(s)	1778
RsaI	GTAC	9 cut(s)	523, 626, 1438, 1559, 2108, 2283, 2300, 3011, 3330
RsaNI	GTAC	9 cut(s)	522, 625, 1437, 1558, 2107, 2282, 2299, 3010, 3329
RseI	CAYNNNNRTG	7 cut(s)	54, 368, 888, 1019, 1398, 1595, 2738
SacI	GAGCTC	1 cut(s)	3370
SalI	GTCGAC	1 cut(s)	146
Sau96I	GGNCC	6 cut(s)	220, 709, 1161, 1940, 1941, 3056
SchI	GAGTC	8 cut(s)	89, 256, 440, 1528, 2101, 2188, 2840, 3194
ScrFI	CCNGG	4 cut(s)	1107, 1845, 2074, 3348
SduI	GDGCHC	8 cut(s)	230, 471, 897, 1113, 1494, 1944, 2264, 3370
SfaNI	GCATC	8 cut(s)	186, 940, 1555, 1587, 1993, 2034, 2302, 2866
SfcI	CTRYAG	1 cut(s)	2337
Sfr274I	CTCGAG	1 cut(s)	470
SinI	GGWCC	3 cut(s)	220, 1161, 3056
SlaI	CTCGAG	1 cut(s)	470
SmiMI	CAYNNNNRTG	7 cut(s)	54, 368, 888, 1019, 1398, 1595, 2738
SmlI	CTYRAG	6 cut(s)	215, 388, 470, 959, 1130, 1731
SmoI	CTYRAG	6 cut(s)	215, 388, 470, 959, 1130, 1731
SphI	GCATGC	1 cut(s)	3355
SsiI	CCGC	6 cut(s)	102, 505, 1667, 2215, 2346, 3507
SspI	AATATT	2 cut(s)	1579, 1897
SspMI	CTAG	7 cut(s)	203, 878, 983, 1142, 1325, 1872, 2658
SstI	GAGCTC	1 cut(s)	3370
StyD4I	CCNGG	4 cut(s)	1105, 1843, 2072, 3346
StyI	CCWWGG	1 cut(s)	202
TaaI	ACNGT	7 cut(s)	526, 616, 2240, 2286, 2646, 3014, 3421
TaiI	ACGT	4 cut(s)	1384, 1818, 3041, 3181
TatI	WGTACW	3 cut(s)	624, 2281, 3009
TfiI	GAWTC	6 cut(s)	1076, 1466, 1789, 2320, 2378, 2471
TscAI	CASTG	6 cut(s)	1065, 1653, 1791, 2056, 2117, 3019
TseFI	GTSAC	4 cut(s)	125, 2651, 3133, 3379
Tsp45I	GTSAC	4 cut(s)	125, 2651, 3133, 3379
TspGWI	ACGGA	5 cut(s)	44, 113, 654, 2373, 2390
TspRI	CASTG	6 cut(s)	1065, 1653, 1791, 2056, 2117, 3019
Van91I	CCANNNNNTGG	1 cut(s)	2750
Vha464I	CTTAAG	2 cut(s)	388, 959
VneI	GTGCAC	1 cut(s)	1490
VpaK11BI	GGWCC	3 cut(s)	220, 1161, 3056
VspI	ATTAAT	1 cut(s)	1308
XagI	CCTNNNNNAGG	2 cut(s)	201, 3150
XapI	RAATTY	6 cut(s)	267, 770, 1001, 2028, 2681, 3098
XbaI	TCTAGA	3 cut(s)	877, 1324, 1871
XceI	RCATGY	5 cut(s)	756, 2795, 3255, 3355, 3396
XhoI	CTCGAG	1 cut(s)	470
XmaJI	CCTAGG	1 cut(s)	202
XmiI	GTMKAC	3 cut(s)	147, 560, 3175
XmnI	GAANNNNTTC	2 cut(s)	171, 2028
XspI	CTAG	7 cut(s)	203, 878, 983, 1142, 1325, 1872, 2658
Zsp2I	ATGCAT	2 cut(s)	2797, 3394

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.