Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr1C

Orthogroup ID

OG0005765

Physical Location & Seq

Genomic Coordinates Forward (+)

32538812 .. 32557364

Gene Structure 18553 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh1CG147600.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3513 bp

ATGTGGCCAGAAATAGAGCCTGCAGATCTTGAAGGAGATTTGCATCTGGGTTTGTGGGATTTGGTTAAGAAAGTGGGATTCTTTTTATGTGGGGGGAGCGCAAGATCAGAGGAGGGCTATGAGATGAAAATGGGAGTGGAATCTGGGGGTGGGATTTGGTTGAGAAATGGGAATTTCATGATACAATGGAGGGAAGATTGGAGAGGCCGACCTCAGAAGGATCGGAGTAGCGAGCCTCTCTATCCTTTTGAATGTCGACAGACTCGAATTTCCTACACGGGTAAATTGCTGGCAGACATTTACTTTCAGCGTGATGATAAGGCTCCTGTCAGAGAGCAGTTAAATTTTGGCCAGTTTCCCCTCATGCTCATGTCAAAGTGTTGCAACTTAAGAGGTCATGATCCTCAGCAGCTAGTTTCTTGCAAAGAAGAGTCATCAGAAATGGGTGGCTACTTCATTTTGAATGGGCTCGAGAGAGTTATTCGACCTGTCATATTGCCGAAGCGGAACTATCCAATGAGTACGGTGCGCAATTCATTTCGTGATCGAAAGGAAGGATATACAGATAAAGCAGTTGTGATAAGATGTGTAAGAGATGATCAGTCTTCAGTAACAGTCAAATTGTATTACCTGCAAAACGGAAGCGCAAGACTTGGTGTTTGGATACAGGGGAAGGAGTTCTTGCTTCCTGTGGGTCTTATTTTAAAGGCTTTTATTGACACTCCTGATCGTGAAATTTTTGCGAGTTTGACATGCTGTTGTATCGAGAAATTTGAGAAAGAAAAAGGTGCTGTAGCCACTCAGCTTGTGGGTGAGAGGGCGCAGATTATTATAAATGAAGTGGCAGACTTGGGTCTTCGCACTCATCTTCAATGTCTAGAACACATTGGCGAGCACTTTCAACCTCTAATGGGTGGACTGGAGAATGAACCTTATTCAGTTGTTGGAGATGCCGTTCTCAAGGACTACATATTTGTTCACCTAGATAAGAATGAAGACAAATTCAATCTGCTCATATTCATGTTGCAGAAACTTTTTTCGCTTATGGATCAGACTTCGGTTCTCGACAACCCAGATTCCTTGCAAAATCAAGAAGTCTTACTCCCTGGCCATCTGATAACCATATATCTCAAGGAAAAACTAGAGGATTGGTTGCAAAGGACCAAAAAACTTCTTCAAGATGAGATGAAAAAGGAAAGCAACAATATCAATTTTGGCAGCGTCGATAGCTTAAAAAAGGTTCTGGAAAAAAATCCTACGAGGCAAATCAGTATGGCGATTGAGAATATGTTGAAAACTGGAAGAATTAATACTCAAACAGGTCTAGATTTGCAGCAGAGGGCGGGCTTTACAGTTCAGGCAGAAAGGCTTAATTTTTTACGATTTTTATCGCATTTTCGTGCTGTTCATCGAGGGGCTTCATTTGCTGGGCTTCGCACCACAAGTGTGCGAAAATTGCTTCCCGAATCCTGGGGTTTCCTTTGCCCTGTGCATACACCTGATGGAGAGCCATGTGGCTTGTTGAACCATATGACTCGCACTTGTAGAATTACATCGTACTTTGATGCACAAGGGAATATTAGAGATTTTTCGAAGATTCGAATGTCTATTCTTAATGTTCTGATCGAAGTTGGAATGACACCTTCACTGCCAATGTTGGTTCATGCTGGTCCTCCTGCGGATCTTTCTGTTCTTTTAGATGGTCGTCTTGTTGGTTATATATCTTCCAAACAAGTTGAGAGAGTTGTGGCTCATTTAAGGAAGCTGAAAGTTTCAGCTGCTTCAGTGATTCCAGATGACTTGGAAGTGGGATACGTTCCTCTCAGCATGGGTGGGGCATATCCTGGTCTCTACCTTAACACTTCTCCTTCTAGATTTGTTCGGCCAGTTAAGAATATTTTGATTCCTGCTGTTGAAGGTCAAAATGTTGAACTCATTGGGCCCTTTGAACAGGTGTTCATGGAAATAAGATGTCCTGATGGTCGAGATGGTGGAAGGAAAGATGCTTTCCCTGCAACTCATGAAGAAATTCATCCAACTGGGATGCTCAGTGTTGTTGCTAATCTTACTCCCTGGTCAGAACATAATCAGAGCCCACGAAATATGTACCAGTGTCAGATGGCAAAACAAACGATGGGTTTCTCATTACAAGCACTTTGCAATCGTGCAGATCAGAAGTTGTATCATCTGAAGACTCCTCAATCTCCCATCGTCCGCACAAAAACGTATACGAATTACTGTGTAGATGAATATCCAACGGGCACAAATGCAATCGTTGCTGTATTGTCGTATACCGGGTATGATATGGAGGATGCTATGATTCTGAGCAAGTCATCTGTAGATCGTGGAATGTTTCATGGACTAGTATATCAGACGGAATCCATTGACTTGTCTGATACAAAGGGTAGTAAATCCCAGAGATCGTTCAGGAGAAGTACAACTGATAAGTCACATCATTCTTTGATCGATTCGGATGGTCTTCCTTATGTGGGTCAGACAATAAAACCAGATGAACCCTATTATAGCACTTATGATGAGATAAAAGGTGCCATAAGAACGACCAAACGAAAGGGGTCGGAGCCTGCTATTGTTGACTATGTGTCTATTGATGGGAAGAAGCATCTTCAGAAGGTGAACATAAGGTTTCGGCACCCTAGAAACCCGATAATTGGTGATAAATTTAGCAGCCGACATGGACAAAAAGGAGTTTGCTCCCAGTTGTGGCCTGACATTGATATGCCATTTTCTGGAGTTACGGGAATGCGCCCAGATCTTATTATCAATCCACATGCATTTCCTTCAAGAATGACAATAGCCATGCTTTTGGAGTCGGTTGCTGCAAAGGGTGGTAGCTTACATGGAAAATATGTTGATGCGACACCATTTGCTAATTCAGAGCAGAAAGTTAATGAAGAAACTAATGGAGAGAAATCCAAAACCCTTGTTGATGAACTTGGTGAAATGTTAAAGGTTAAAGGGTTTAATTACCATGGTGCAGAGGTATTGTACAGTGGCGTCTATGGAACAGAACTCACGTGTGAAATCTTTATTGGTCCTGTTTACTATCAACGATTACGGCATATGGTTTCAGACAAATTTCAGGTTCGCTCTACGGGAATGGTAGATCAAGTCACACGCCAGCCTATCAAAGGACGAAAACGAGGTGGCGGTATACGTTTTGGTGAAATGGAGAGAGACTCCTTGCTTGCACATGGGGCAGCATATTTGTTGCATGATAGACTTCATACATGTTCTGATTATCACATTGCTGACGTATGTTCCCTTTGTGGATCCATCCTAACAACCTCATCAGTCCAGCCACAAATACGGGTTCGTGAAATGCGGGGCTTGCCCCCTATGAGAGCTCCCAAGAAGGTGACTTGCCATGCATGCAAGTCGAGTAAAGGGATGGAGACGGTTGCAATGCCTTATGTCTTTAGATATTTAGCAGCTGAGTTAGCAGCTATGAACATAAAACTGACCCTCCAGTTGAGCAATGCAACTGGAGCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000428 GO:0003674 GO:0003824 GO:0003899 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005736 GO:0005737 GO:0005829 GO:0006139 GO:0006351 GO:0006360 GO:0006725 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009553 GO:0009561 GO:0009987 GO:0010467 GO:0016070 GO:0016740 GO:0016772 GO:0016779 GO:0018130 GO:0019438 GO:0030880 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032774 GO:0032991 GO:0034062 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044452 GO:0044464 GO:0046483 GO:0048229 GO:0048856 GO:0055029 GO:0061695 GO:0070013 GO:0071704 GO:0090304 GO:0097659 GO:0097747 GO:0140098 GO:1901360 GO:1901362 GO:1901576 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko00230 ko00240 ko01100 ko03020 map00230 map00240 map01100 map03020

Pfam Domains

Protein Families

RNA_pol_Rpa2_4 RNA_pol_Rpb2_1 RNA_pol_Rpb2_2 RNA_pol_Rpb2_3 RNA_pol_Rpb2_6 RNA_pol_Rpb2_7

Protein Analysis

1170

Amino Acids

131.61

Weight (kDa)

8.6

Isoelectric Point (pI)

36.7

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RNA_pol_Rpb2_1	PF04563	51 - 401	6.3e-27	RNA polymerase beta subunit
RNA_pol_Rpb2_2	PF04561	194 - 366	5.3e-10	RNA polymerase Rpb2, domain 2
RNA_pol_Rpb2_3	PF04565	454 - 517	1.2e-27	RNA polymerase Rpb2, domain 3
RNA_pol_Rpa2_4	PF06883	571 - 628	3.7e-21	RNA polymerase I, Rpa2 specific domain
RNA_pol_Rpb2_6	PF00562	686 - 1054	1.8e-121	RNA polymerase Rpb2, domain 6
RNA_pol_Rpb2_7	PF04560	1056 - 1163	1e-21	RNA polymerase Rpb2, domain 7

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0005765)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G29940
fragaria_vesca	FvH4_5g25870 FvH4_5g25870 FvH4_7g07420 FvH4_7g07420 FvH4_7g07420 FvH4_7g07420 FvH4_7g07420 FvH4_7g07420 FvH4_7g07420
malus_domestica	MD08G1237100.v1.1 MD15G1428900.v1.1
prunus_persica	Prupe.1G574600_v2.0.a1 Prupe.1G574600_v2.0.a1 Prupe.7G107000_v2.0.a1 Prupe.7G184700_v2.0.a1 Prupe.7G223000_v2.0.a1
pyrus_communis	pycom08g20550
rosa_chinensis	RchiOBHm_Chr1g0339571 RchiOBHm_Chr1g0339591
rosa_laevigata	RLG00000029239
rosa_multiflora	Rmu_sc0015580.1_g000004
rosa_roxburghii	Rroxscaffold_4G00313560 Rroxscaffold_4G00313600
rosa_rugosa	Rorug01G0140100.1
rosa_samantha	Rh1AG158100 Rh1CG147600
rosa_wichuraiana	Rw1G013100

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	833
Acc16I	TGCGCA	1 cut(s)	530
Acc36I	ACCTGC	1 cut(s)	639
AccB1I	GGYRCC	2 cut(s)	2546, 2649
AccB7I	CCANNNNNTGG	1 cut(s)	2747
AccI	GTMKAC	4 cut(s)	256, 2228, 2291, 3172
AciI	CCGC	6 cut(s)	505, 1343, 1679, 2215, 3168, 3343
AclWI	GGATC	6 cut(s)	228, 395, 1056, 1689, 3285, 3298
AcoI	YGGCCR	4 cut(s)	5, 349, 1108, 1883
AcsI	RAATTY	9 cut(s)	172, 267, 343, 735, 770, 1001, 2028, 2678, 3095
AcuI	CTGAAG	4 cut(s)	591, 1767, 2210, 2609
AcvI	CACGTG	1 cut(s)	3036
AcyI	GRCGYC	1 cut(s)	3015
AfaI	GTAC	5 cut(s)	523, 1559, 2108, 2437, 3008
AfiI	CCNNNNNNNGG	7 cut(s)	911, 1470, 2489, 2669, 2721, 2747, 3149
AflII	CTTAAG	1 cut(s)	388
AflIII	ACRYGT	2 cut(s)	3035, 3248
AhdI	GACNNNNNGTC	1 cut(s)	2599
AhlI	ACTAGT	1 cut(s)	2362
AjnI	CCWGG	4 cut(s)	1105, 1469, 1843, 2072
AjuI	GAANNNNNNNTTGG	2 cut(s)	3033, 3065
AleI	CACNNNNGTG	1 cut(s)	1445
AloI	GAACNNNNNNTCC	2 cut(s)	939, 971
Alw21I	GWGCWC	2 cut(s)	897, 3367
Alw26I	GTCTC	3 cut(s)	1853, 3189, 3407
AlwI	GGATC	6 cut(s)	228, 395, 1056, 1689, 3285, 3298
Ama87I	CYCGRG	1 cut(s)	470
AoxI	GGCC	7 cut(s)	5, 205, 349, 1108, 1883, 1940, 2723
ApaI	GGGCCC	1 cut(s)	1944
ApeKI	GCWGC	9 cut(s)	409, 1218, 1333, 1778, 2685, 2837, 3218, 3449, 3461
ApoI	RAATTY	9 cut(s)	172, 267, 343, 735, 770, 1001, 2028, 2678, 3095
ArsI	GACNNNNNNTTYG	4 cut(s)	642, 674, 956, 988
AseI	ATTAAT	1 cut(s)	1308
Asp700I	GAANNNNTTC	2 cut(s)	677, 2028
AspLEI	GCGC	5 cut(s)	101, 531, 647, 823, 2766
AspS9I	GGNCC	5 cut(s)	1161, 1670, 1940, 1941, 3053
AsuC2I	CCSGG	1 cut(s)	2296
AsuHPI	GGTGA	7 cut(s)	824, 971, 2644, 2684, 2969, 3194, 3388
AsuII	TTCGAA	2 cut(s)	1592, 1600
AvaI	CYCGRG	1 cut(s)	470
AvaII	GGWCC	3 cut(s)	1161, 1670, 3053
BaeGI	GKGCMC	2 cut(s)	1944, 2264
BalI	TGGCCA	3 cut(s)	7, 351, 1110
BamHI	GGATCC	1 cut(s)	3290
BanI	GGYRCC	2 cut(s)	2546, 2649
BanII	GRGCYC	4 cut(s)	471, 1944, 2096, 3367
BarI	GAAGNNNNNNTAC	2 cut(s)	1294, 1326
BbrPI	CACGTG	1 cut(s)	3036
BbsI	GAAGAC	5 cut(s)	597, 848, 1002, 2198, 2471
Bbv12I	GWGCWC	2 cut(s)	897, 3367
BbvCI	CCTCAGC	1 cut(s)	405
BbvI	GCAGC	9 cut(s)	421, 1230, 1345, 1765, 2697, 2824, 3230, 3461, 3473
BceAI	ACGGC	2 cut(s)	938, 3092
BcgI	CGANNNNNNTGC	2 cut(s)	3378, 3412
BciT130I	CCWGG	4 cut(s)	1107, 1471, 1845, 2074
BciVI	GTATCC	2 cut(s)	657, 1805
BclI	TGATCA	1 cut(s)	598
BcnI	CCSGG	1 cut(s)	2296
BcoDI	GTCTC	3 cut(s)	1853, 3189, 3407
BcuI	ACTAGT	1 cut(s)	2362
BfaI	CTAG	8 cut(s)	413, 878, 983, 1142, 1325, 1872, 2363, 2655
BfmI	CTRYAG	3 cut(s)	21, 792, 2337
BfrI	CTTAAG	1 cut(s)	388
BfuAI	ACCTGC	1 cut(s)	639
BfuI	GTATCC	2 cut(s)	657, 1805
BglII	AGATCT	2 cut(s)	25, 2770
BisI	GCNGC	9 cut(s)	410, 1219, 1334, 1779, 2686, 2838, 3219, 3450, 3462
BlsI	GCNGC	9 cut(s)	411, 1220, 1335, 1780, 2687, 2839, 3220, 3451, 3463
Bme1390I	CCNGG	5 cut(s)	1107, 1471, 1845, 2074, 2296
Bme18I	GGWCC	3 cut(s)	1161, 1670, 3053
BmeRI	GACNNNNNGTC	1 cut(s)	2599
BmeT110I	CYCGRG	1 cut(s)	470
BmgT120I	GGNCC	5 cut(s)	1161, 1670, 1940, 1941, 3053
BmiI	GGNNCC	6 cut(s)	324, 1942, 2548, 2580, 2651, 3292
BmrFI	CCNGG	5 cut(s)	1107, 1471, 1845, 2074, 2296
BmrI	ACTGGG	2 cut(s)	2049, 2710
BmsI	GCATC	8 cut(s)	52, 940, 1555, 1993, 2034, 2302, 2629, 2863
BmuI	ACTGGG	2 cut(s)	2049, 2710
BoxI	GACNNNNGTC	1 cut(s)	852
BpiI	GAAGAC	5 cut(s)	597, 848, 1002, 2198, 2471
BplI	GAGNNNNNCTC	2 cut(s)	3182, 3214
BpmI	CTGGAG	3 cut(s)	941, 2769, 3470
Bpu10I	CCTNAGC	1 cut(s)	405
Bpu14I	TTCGAA	2 cut(s)	1592, 1600
BpuEI	CTTGAG	2 cut(s)	944, 1115
BpuMI	CCSGG	1 cut(s)	2296
Bsa29I	ATCGAT	1 cut(s)	2466
BsaAI	YACGTR	1 cut(s)	3036
BsaBI	GATNNNNATC	3 cut(s)	42, 1387, 2250
BsaHI	GRCGYC	1 cut(s)	3015
BsaI	GGTCTC	1 cut(s)	1853
BsaJI	CCNNGG	4 cut(s)	1105, 1470, 2072, 2989
BsaXI	ACNNNNNCTCC	4 cut(s)	939, 969, 3468, 3498
Bsc4I	CCNNNNNNNGG	7 cut(s)	911, 1470, 2489, 2669, 2721, 2747, 3149
Bse1I	ACTGG	9 cut(s)	352, 924, 1303, 1886, 2044, 2110, 2716, 3487, 3508
Bse3DI	GCAATG	3 cut(s)	3264, 3429, 3502
Bse8I	GATNNNNATC	3 cut(s)	42, 1387, 2250
BseBI	CCWGG	4 cut(s)	1107, 1471, 1845, 2074
BseCI	ATCGAT	1 cut(s)	2466
BseDI	CCNNGG	4 cut(s)	1105, 1470, 2072, 2989
BseGI	GGATG	6 cut(s)	2032, 2049, 2317, 2479, 3294, 3414
BseJI	GATNNNNATC	3 cut(s)	42, 1387, 2250
BseLI	CCNNNNNNNGG	7 cut(s)	911, 1470, 2489, 2669, 2721, 2747, 3149
BseMI	GCAATG	3 cut(s)	3264, 3429, 3502
BseMII	CTCAG	7 cut(s)	227, 419, 815, 1837, 2062, 2315, 3444
BseNI	ACTGG	9 cut(s)	352, 924, 1303, 1886, 2044, 2110, 2716, 3487, 3508
BseRI	GAGGAG	2 cut(s)	125, 2187
BseSI	GKGCMC	2 cut(s)	1944, 2264
BseXI	GCAGC	9 cut(s)	421, 1230, 1345, 1765, 2697, 2824, 3230, 3461, 3473
BseYI	CCCAGC	1 cut(s)	1427
BsgI	GTGCAG	2 cut(s)	2187, 3015
BshFI	GGCC	7 cut(s)	7, 207, 351, 1110, 1885, 1942, 2725
BshNI	GGYRCC	2 cut(s)	2546, 2649
BshVI	ATCGAT	1 cut(s)	2466
BsiHKAI	GWGCWC	2 cut(s)	897, 3367
BsiHKCI	CYCGRG	1 cut(s)	470
BsiSI	CCGG	1 cut(s)	2295
BslI	CCNNNNNNNGG	7 cut(s)	911, 1470, 2489, 2669, 2721, 2747, 3149
BsmAI	GTCTC	3 cut(s)	1853, 3189, 3407
BsmBI	CGTCTC	1 cut(s)	3407
BsmI	GAATGC	1 cut(s)	2766
BsnI	GGCC	7 cut(s)	7, 207, 351, 1110, 1885, 1942, 2725
Bso31I	GGTCTC	1 cut(s)	1853
BsoBI	CYCGRG	1 cut(s)	470
Bsp119I	TTCGAA	2 cut(s)	1592, 1600
Bsp120I	GGGCCC	1 cut(s)	1940
Bsp1286I	GDGCHC	6 cut(s)	471, 897, 1944, 2096, 2264, 3367
Bsp1407I	TGTACA	1 cut(s)	3006
Bsp19I	CCATGG	1 cut(s)	2989
BspACI	CCGC	6 cut(s)	505, 1343, 1679, 2215, 3168, 3343
BspANI	GGCC	7 cut(s)	7, 207, 351, 1110, 1885, 1942, 2725
BspCNI	CTCAG	7 cut(s)	226, 418, 814, 1836, 2061, 2316, 3445
BspDI	ATCGAT	1 cut(s)	2466
BspHI	TCATGA	3 cut(s)	177, 397, 2020
BspLI	GGNNCC	6 cut(s)	324, 1942, 2548, 2580, 2651, 3292
BspMAI	CTGCAG	1 cut(s)	25
BspMI	ACCTGC	1 cut(s)	639
BspPI	GGATC	6 cut(s)	228, 395, 1056, 1689, 3285, 3298
BspT104I	TTCGAA	2 cut(s)	1592, 1600
BspT107I	GGYRCC	2 cut(s)	2546, 2649
BspTI	CTTAAG	1 cut(s)	388
BspTNI	GGTCTC	1 cut(s)	1853
BsrDI	GCAATG	3 cut(s)	3264, 3429, 3502
BsrGI	TGTACA	1 cut(s)	3006
BsrI	ACTGG	9 cut(s)	352, 924, 1303, 1886, 2044, 2110, 2716, 3487, 3508
BssECI	CCNNGG	4 cut(s)	1105, 1470, 2072, 2989
BssNAI	GTATAC	3 cut(s)	2229, 2292, 3173
BssNI	GRCGYC	1 cut(s)	3015
BssT1I	CCWWGG	1 cut(s)	2989
Bst1107I	GTATAC	3 cut(s)	2229, 2292, 3173
Bst2UI	CCWGG	4 cut(s)	1107, 1471, 1845, 2074
Bst4CI	ACNGT	6 cut(s)	526, 616, 1354, 2240, 3011, 3418
Bst6I	CTCTTC	1 cut(s)	423
BstACI	GRCGYC	1 cut(s)	3015
BstAFI	CTTAAG	1 cut(s)	388
BstAPI	GCANNNNNTGC	1 cut(s)	2276
BstAUI	TGTACA	1 cut(s)	3006
BstBAI	YACGTR	1 cut(s)	3036
BstBI	TTCGAA	2 cut(s)	1592, 1600
BstDEI	CTNAG	7 cut(s)	213, 405, 801, 1823, 2048, 2324, 3453
BstDSI	CCRYGG	1 cut(s)	2989
BstENI	CCTNNNNNAGG	1 cut(s)	3147
BstF5I	GGATG	6 cut(s)	2032, 2049, 2317, 2479, 3294, 3414
BstHHI	GCGC	5 cut(s)	101, 531, 647, 823, 2766
BstMAI	GTCTC	3 cut(s)	1853, 3189, 3407
BstNI	CCWGG	4 cut(s)	1107, 1471, 1845, 2074
BstNSI	RCATGY	4 cut(s)	756, 2792, 3252, 3393
BstPAI	GACNNNNGTC	1 cut(s)	852
BstSCI	CCNGG	5 cut(s)	1105, 1469, 1843, 2072, 2294
BstSFI	CTRYAG	3 cut(s)	21, 792, 2337
BstSLI	GKGCMC	2 cut(s)	1944, 2264
BstV1I	GCAGC	9 cut(s)	421, 1230, 1345, 1765, 2697, 2824, 3230, 3461, 3473
BstV2I	GAAGAC	5 cut(s)	597, 848, 1002, 2198, 2471
BstX2I	RGATCY	4 cut(s)	25, 1681, 2770, 3290
BstXI	CCANNNNNNTGG	1 cut(s)	2824
BstYI	RGATCY	4 cut(s)	25, 1681, 2770, 3290
BstZ17I	GTATAC	3 cut(s)	2229, 2292, 3173
Bsu15I	ATCGAT	1 cut(s)	2466
BsuI	GTATCC	2 cut(s)	657, 1805
BsuRI	GGCC	7 cut(s)	7, 207, 351, 1110, 1885, 1942, 2725
BsuTUI	ATCGAT	1 cut(s)	2466
BtgI	CCRYGG	1 cut(s)	2989
BtsCI	GGATG	6 cut(s)	2032, 2049, 2317, 2479, 3294, 3414
BtsI	GCAGTG	1 cut(s)	1646
BtsIMutI	CAGTG	5 cut(s)	1646, 1791, 2056, 2117, 3016
BveI	ACCTGC	1 cut(s)	639
CciI	TCATGA	3 cut(s)	177, 397, 2020
CfoI	GCGC	5 cut(s)	101, 531, 647, 823, 2766
Cfr13I	GGNCC	5 cut(s)	1161, 1670, 1940, 1941, 3053
ClaI	ATCGAT	1 cut(s)	2466
CseI	GACGC	2 cut(s)	1210, 3004
Csp6I	GTAC	5 cut(s)	522, 1558, 2107, 2436, 3007
CspCI	CAANNNNNGTGG	2 cut(s)	787, 822
CviQI	GTAC	5 cut(s)	522, 1558, 2107, 2436, 3007
DdeI	CTNAG	7 cut(s)	213, 405, 801, 1823, 2048, 2324, 3453
DraI	TTTAAA	1 cut(s)	705
DriI	GACNNNNNGTC	1 cut(s)	2599
EaeI	YGGCCR	4 cut(s)	5, 349, 1108, 1883
Eam1104I	CTCTTC	1 cut(s)	423
Eam1105I	GACNNNNNGTC	1 cut(s)	2599
EarI	CTCTTC	1 cut(s)	423
Ecl136II	GAGCTC	1 cut(s)	3365
Eco130I	CCWWGG	1 cut(s)	2989
Eco24I	GRGCYC	4 cut(s)	471, 1944, 2096, 3367
Eco31I	GGTCTC	1 cut(s)	1853
Eco47I	GGWCC	3 cut(s)	1161, 1670, 3053
Eco53kI	GAGCTC	1 cut(s)	3365
Eco57I	CTGAAG	4 cut(s)	591, 1767, 2210, 2609
Eco72I	CACGTG	1 cut(s)	3036
Eco88I	CYCGRG	1 cut(s)	470
EcoICRI	GAGCTC	1 cut(s)	3365
EcoNI	CCTNNNNNAGG	1 cut(s)	3147
EcoO109I	RGGNCCY	1 cut(s)	1941
EcoRII	CCWGG	4 cut(s)	1105, 1469, 1843, 2072
EcoT14I	CCWWGG	1 cut(s)	2989
EcoT22I	ATGCAT	2 cut(s)	2794, 3391
EcoT38I	GRGCYC	4 cut(s)	471, 1944, 2096, 3367
ErhI	CCWWGG	1 cut(s)	2989
Esp3I	CGTCTC	1 cut(s)	3407
FalI	AAGNNNNNCTT	2 cut(s)	665, 697
FauI	CCCGC	2 cut(s)	1336, 3336
FauNDI	CATATG	2 cut(s)	1530, 3081
FbaI	TGATCA	1 cut(s)	598
FblI	GTMKAC	4 cut(s)	256, 2228, 2291, 3172
Fnu4HI	GCNGC	9 cut(s)	410, 1219, 1334, 1779, 2686, 2838, 3219, 3450, 3462
FokI	GGATG	6 cut(s)	2019, 2056, 2324, 2486, 3281, 3421
FriOI	GRGCYC	4 cut(s)	471, 1944, 2096, 3367
Fsp4HI	GCNGC	9 cut(s)	410, 1219, 1334, 1779, 2686, 2838, 3219, 3450, 3462
FspBI	CTAG	8 cut(s)	413, 878, 983, 1142, 1325, 1872, 2363, 2655
FspI	TGCGCA	1 cut(s)	530
GlaI	GCGC	5 cut(s)	100, 530, 646, 822, 2765
GluI	GCNGC	9 cut(s)	410, 1219, 1334, 1779, 2686, 2838, 3219, 3450, 3462
GsaI	CCCAGC	1 cut(s)	1431
GsuI	CTGGAG	3 cut(s)	941, 2769, 3470
HaeIII	GGCC	7 cut(s)	7, 207, 351, 1110, 1885, 1942, 2725
HapII	CCGG	1 cut(s)	2295
HgaI	GACGC	2 cut(s)	1210, 3004
HhaI	GCGC	5 cut(s)	101, 531, 647, 823, 2766
Hin1I	GRCGYC	1 cut(s)	3015
Hin6I	GCGC	5 cut(s)	99, 529, 645, 821, 2764
HinP1I	GCGC	5 cut(s)	99, 529, 645, 821, 2764
HincII	GTYRAC	2 cut(s)	257, 2593
HindII	GTYRAC	2 cut(s)	257, 2593
HpaII	CCGG	1 cut(s)	2295
HphI	GGTGA	7 cut(s)	824, 971, 2644, 2684, 2969, 3194, 3388
Hpy166II	GTNNAC	9 cut(s)	257, 917, 979, 2229, 2292, 2593, 2635, 3061, 3173
Hpy8I	GTNNAC	9 cut(s)	257, 917, 979, 2229, 2292, 2593, 2635, 3061, 3173
Hpy99I	CGWCG	1 cut(s)	1226
HpyCH4III	ACNGT	6 cut(s)	526, 616, 1354, 2240, 3011, 3418
HpyCH4IV	ACGT	5 cut(s)	1815, 2225, 3035, 3175, 3273
HpyF3I	CTNAG	7 cut(s)	213, 405, 801, 1823, 2048, 2324, 3453
HpySE526I	ACGT	5 cut(s)	1815, 2225, 3035, 3175, 3273
Hsp92I	GRCGYC	1 cut(s)	3015
HspAI	GCGC	5 cut(s)	99, 529, 645, 821, 2764
Ksp22I	TGATCA	1 cut(s)	598
LmnI	GCTCC	6 cut(s)	96, 328, 2578, 2717, 3370, 3506
Lsp1109I	GCAGC	9 cut(s)	421, 1230, 1345, 1765, 2697, 2824, 3230, 3461, 3473
LweI	GCATC	8 cut(s)	52, 940, 1555, 1993, 2034, 2302, 2629, 2863
MaeI	CTAG	8 cut(s)	413, 878, 983, 1142, 1325, 1872, 2363, 2655
MaeII	ACGT	5 cut(s)	1815, 2225, 3035, 3175, 3273
MaeIII	GTNAC	5 cut(s)	610, 2448, 2752, 3130, 3376
MflI	RGATCY	4 cut(s)	25, 1681, 2770, 3290
MhlI	GDGCHC	6 cut(s)	471, 897, 1944, 2096, 2264, 3367
MlsI	TGGCCA	3 cut(s)	7, 351, 1110
MluNI	TGGCCA	3 cut(s)	7, 351, 1110
MlyI	GAGTC	6 cut(s)	256, 440, 1528, 2188, 2837, 3191
MmeI	TCCRAC	5 cut(s)	925, 1612, 2060, 2279, 2556
Mox20I	TGGCCA	3 cut(s)	7, 351, 1110
Mph1103I	ATGCAT	2 cut(s)	2794, 3391
MroXI	GAANNNNTTC	2 cut(s)	677, 2028
MscI	TGGCCA	3 cut(s)	7, 351, 1110
MslI	CAYNNNNRTG	5 cut(s)	368, 1019, 1398, 1445, 2735
Msp20I	TGGCCA	3 cut(s)	7, 351, 1110
MspA1I	CMGCKG	2 cut(s)	1778, 3452
MspCI	CTTAAG	1 cut(s)	388
MspI	CCGG	1 cut(s)	2295
MspR9I	CCNGG	5 cut(s)	1107, 1471, 1845, 2074, 2296
Mva1269I	GAATGC	1 cut(s)	2766
MvaI	CCWGG	4 cut(s)	1107, 1471, 1845, 2074
NciI	CCSGG	1 cut(s)	2296
NcoI	CCATGG	1 cut(s)	2989
NdeI	CATATG	2 cut(s)	1530, 3081
NlaIV	GGNNCC	6 cut(s)	324, 1942, 2548, 2580, 2651, 3292
NmuCI	GTSAC	3 cut(s)	2448, 3130, 3376
NsbI	TGCGCA	1 cut(s)	530
NsiI	ATGCAT	2 cut(s)	2794, 3391
NspI	RCATGY	4 cut(s)	756, 2792, 3252, 3393
NspV	TTCGAA	2 cut(s)	1592, 1600
OliI	CACNNNNGTG	1 cut(s)	1445
PaeI	GCATGC	1 cut(s)	3393
PaeR7I	CTCGAG	1 cut(s)	470
PagI	TCATGA	3 cut(s)	177, 397, 2020
PciI	ACATGT	1 cut(s)	3248
PcsI	WCGNNNNNNNCGW	1 cut(s)	262
PctI	GAATGC	1 cut(s)	2766
PdmI	GAANNNNTTC	2 cut(s)	677, 2028
PflMI	CCANNNNNTGG	1 cut(s)	2747
PkrI	GCNGC	9 cut(s)	411, 1220, 1335, 1780, 2687, 2839, 3220, 3451, 3463
PleI	GAGTC	6 cut(s)	256, 439, 1528, 2188, 2836, 3191
PmaCI	CACGTG	1 cut(s)	3036
PmlI	CACGTG	1 cut(s)	3036
PpsI	GAGTC	6 cut(s)	256, 439, 1528, 2188, 2836, 3191
Ppu21I	YACGTR	1 cut(s)	3036
PscI	ACATGT	1 cut(s)	3248
PshAI	GACNNNNGTC	1 cut(s)	852
PshBI	ATTAAT	1 cut(s)	1308
PsiI	TTATAA	1 cut(s)	833
Psp124BI	GAGCTC	1 cut(s)	3367
Psp6I	CCWGG	4 cut(s)	1105, 1469, 1843, 2072
PspCI	CACGTG	1 cut(s)	3036
PspFI	CCCAGC	1 cut(s)	1427
PspGI	CCWGG	4 cut(s)	1105, 1469, 1843, 2072
PspN4I	GGNNCC	6 cut(s)	324, 1942, 2548, 2580, 2651, 3292
PspOMI	GGGCCC	1 cut(s)	1940
PspPI	GGNCC	5 cut(s)	1161, 1670, 1940, 1941, 3053
PstI	CTGCAG	1 cut(s)	25
PsuI	RGATCY	4 cut(s)	25, 1681, 2770, 3290
PvuII	CAGCTG	2 cut(s)	1778, 3452
RsaI	GTAC	5 cut(s)	523, 1559, 2108, 2437, 3008
RsaNI	GTAC	5 cut(s)	522, 1558, 2107, 2436, 3007
RseI	CAYNNNNRTG	5 cut(s)	368, 1019, 1398, 1445, 2735
SacI	GAGCTC	1 cut(s)	3367
SalI	GTCGAC	1 cut(s)	255
SatI	GCNGC	9 cut(s)	410, 1219, 1334, 1779, 2686, 2838, 3219, 3450, 3462
Sau96I	GGNCC	5 cut(s)	1161, 1670, 1940, 1941, 3053
SchI	GAGTC	6 cut(s)	256, 440, 1528, 2188, 2837, 3191
ScrFI	CCNGG	5 cut(s)	1107, 1471, 1845, 2074, 2296
SduI	GDGCHC	6 cut(s)	471, 897, 1944, 2096, 2264, 3367
SfaNI	GCATC	8 cut(s)	52, 940, 1555, 1993, 2034, 2302, 2629, 2863
SfcI	CTRYAG	3 cut(s)	21, 792, 2337
Sfr274I	CTCGAG	1 cut(s)	470
SfuI	TTCGAA	2 cut(s)	1592, 1600
SinI	GGWCC	3 cut(s)	1161, 1670, 3053
SlaI	CTCGAG	1 cut(s)	470
SmiMI	CAYNNNNRTG	5 cut(s)	368, 1019, 1398, 1445, 2735
SmlI	CTYRAG	4 cut(s)	388, 470, 959, 1130
SmoI	CTYRAG	4 cut(s)	388, 470, 959, 1130
SpeI	ACTAGT	1 cut(s)	2362
SphI	GCATGC	1 cut(s)	3393
SsiI	CCGC	6 cut(s)	505, 1343, 1679, 2215, 3168, 3343
SspI	AATATT	2 cut(s)	1579, 1897
SspMI	CTAG	8 cut(s)	413, 878, 983, 1142, 1325, 1872, 2363, 2655
SstI	GAGCTC	1 cut(s)	3367
StyD4I	CCNGG	5 cut(s)	1105, 1469, 1843, 2072, 2294
StyI	CCWWGG	1 cut(s)	2989
TaaI	ACNGT	6 cut(s)	526, 616, 1354, 2240, 3011, 3418
TaiI	ACGT	5 cut(s)	1818, 2228, 3038, 3178, 3276
TatI	WGTACW	2 cut(s)	2435, 3006
TscAI	CASTG	5 cut(s)	1653, 1791, 2056, 2117, 3016
TseFI	GTSAC	3 cut(s)	2448, 3130, 3376
TseI	GCWGC	9 cut(s)	409, 1218, 1333, 1778, 2685, 2837, 3218, 3449, 3461
Tsp45I	GTSAC	3 cut(s)	2448, 3130, 3376
TspGWI	ACGGA	2 cut(s)	654, 2390
TspRI	CASTG	5 cut(s)	1653, 1791, 2056, 2117, 3016
Van91I	CCANNNNNTGG	1 cut(s)	2747
Vha464I	CTTAAG	1 cut(s)	388
VpaK11BI	GGWCC	3 cut(s)	1161, 1670, 3053
VspI	ATTAAT	1 cut(s)	1308
XagI	CCTNNNNNAGG	1 cut(s)	3147
XapI	RAATTY	9 cut(s)	172, 267, 343, 735, 770, 1001, 2028, 2678, 3095
XbaI	TCTAGA	3 cut(s)	877, 1324, 1871
XceI	RCATGY	4 cut(s)	756, 2792, 3252, 3393
XcmI	CCANNNNNNNNNTGG	1 cut(s)	805
XhoI	CTCGAG	1 cut(s)	470
XmiI	GTMKAC	4 cut(s)	256, 2228, 2291, 3172
XmnI	GAANNNNTTC	2 cut(s)	677, 2028
XspI	CTAG	8 cut(s)	413, 878, 983, 1142, 1325, 1872, 2363, 2655
Zsp2I	ATGCAT	2 cut(s)	2794, 3391

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.