Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr1A

Orthogroup ID

OG0005765

Physical Location & Seq

Genomic Coordinates Forward (+)

30042613 .. 30058294

Gene Structure 15682 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh1AG158100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3414 bp

ATGCAGCAGAAGAGGAGAACCTCCGCCGGAGACTTAAGCCACTTGCGCGAGCTGTTCCGGCACCACATAGATATATTACTCTGGTTCGCTGACGATCCTATTCTAGGCCGACCTCAGAAGGATCGGAGTAGCGAGCCTCTCTATCCTTTTGAATGTCGACAGACTCGAATTTCCTACACGGGTAAATTGCTGGCAGACATTTACTTTCAGCGTGATGATAAGGCTCCTGTCAGAGAGCAGTTAAATTTTGGCCAGTTTCCCCTCATGCTCATGTCAAAGTGTTGCAACTTAAGAGGTCATGATCCTCAGCAGCTAGTTTCTTGCAAAGAAGAGTCATCAGAAATGGGTGGCTACTTCATTTTGAATGGGCTCGAGAGAGTTATTCGACCTGTCATATTGCCGAAGCGGAACTATCCAATGAGTACGGTGCGCAATTCATTTCGTGATCGAAAGGAAGGATATACAGATAAAGCAGTTGTGATAAGATGTGTAAGAGATGATCAGTCTTCAGTAACAGTCAAATTGTATTACCTGCAAAACGGAAGCGCAAGACTTGGTGTTTGGATACAGGGGAAGGAGTTCTTGCTTCCTGTGGGTCTTATTTTAAAGGCTTTTATTGACACTCCTGATCGTGAAATTTTTGCGAGTTTGACATGCTGTTGTATCGAGAAATTTGAGAAAGAAAAAGGTGCTGTAGCCACTCAGCTTGTGGGTGAGAGGGCGCAGATTATTATAAATGAAGTGGCAGACTTGGGTCTTCGCACTCATCTTCAATGTCTAGAACACATTGGCGAGCACTTTCAACCTCTAATGGGTGGACTGGAGAATGAACCTTATTCAGTTGTTGGAGATGCCGTTCTCAAGGACTACATATTTGTTCACCTAGATAAGAATGAAGACAAATTCAATCTGCTCATATTCATGTTGCAGAAACTTTTTTCGCTTATGGATCAGACTTCGGTTCTCGACAACCCAGATTCCTTGCAAAATCAAGAAGTCTTACTCCCTGGCCATCTGATAACCATATATCTCAAGGAAAAACTAGAGGATTGGTTGCAAAGGACCAAAAAACTTCTTCAAGATGAGATGAAAAAGGAAAGCAACAATATCAATTTTGGCAGCGTCGATAGCTTAAAAAAGGTTCTGGAAAAAAATCCTACGAGGCAAATCAGTATGGCGATTGAGAATATGTTGAAAACTGGAAGAATTAATACTCAAACAGGTCTAGATTTGCAGCAGAGGGCGGGCTTTACAGTTCAGGCAGAAAGGCTTAATTTTTTACGATTTTTATCGCATTTTCGTGCTGTTCATCGAGGGGCTTCATTTGCTGGGCTTCGCACCACAAGTGTGCGAAAATTGCTTCCCGAATCCTGGGGTTTCCTTTGCCCTGTGCATACACCTGATGGAGAGCCATGTGGCTTGTTGAACCATATGACTCGCACTTGTAGAATTACATCGTACTTTGATGCACAAGGGAATATTAGAGATTTTTCGAAGATTCGAATGTCTATTCTTAATGTTCTGATCGAAGTTGGAATGACACCTTCACTGCCAATGTTGGTTCATGCTGGTCCTCCTGCGGATCTTTCTGTTCTTTTAGATGGTCGTCTTGTTGGTTATATATCTTCCAAACAAGTTGAGAGAGTTGTGGCTCATTTAAGGAAGCTGAAAGTTTCAGCTGCTTCAGTGATTCCAGATGACTTGGAAGTGGGATACGTTCCTCTCAGCATGGGTGGGGCATATCCTGGTCTCTACCTTAACACTTCTCCTTCTAGATTTGTTCGGCCAGTTAAGAATATTTTGATTCCTGCTGTTGAAGGTCAAAATGTTGAACTCATTGGGCCCTTTGAACAGGTGTTCATGGAAATAAGATGTCCTGATGGTCGAGATGGTGGAAGGAAAGATGCTTTCCCTGCAACTCATGAAGAAATTCATCCAACTGGGATGCTCAGTGTTGTTGCTAATCTTACTCCCTGGTCAGAACATAATCAGAGCCCACGAAATATGTACCAGTGTCAGATGGCAAAACAAACGATGGGTTTCTCATTACAAGCACTTTGCAATCGTGCAGATCAGAAGTTGTATCATCTGAAGACTCCTCAATCTCCCATCGTCCGCACAAAAACGTATACGAATTACTGTGTAGATGAATATCCAACGGGCACAAATGCAATCGTTGCTGTATTGTCGTATACCGGGTATGATATGGAGGATGCTATGATTCTGAGCAAGTCATCTGTAGATCGTGGAATGTTTCATGGACTAGTATATCAGACGGAATCCATTGACTTGTCTGATACAAAGGGTAGTAAATCCCAGAGATCGTTCAGGAGAAGTACAACTGATAAGTCACATCATTCTTTGATCGATTCGGATGGTCTTCCTTATGTGGGTCAGACAATAAAACCAGATGAACCCTATTATAGCACTTATGATGAGATAAAAGGTGCCATAAGAACGACCAAACGAAAGGGGTCGGAGCCTGCTATTGTTGACTATGTGTCTATTGATGGGAAGAAGCATCTTCAGAAGGTGAACATAAGGTTTCGGCACCCTAGAAACCCGATAATTGGTGATAAATTTAGCAGCCGACATGGACAAAAAGGAGTTTGCTCCCAGTTGTGGCCTGACATTGATATGCCATTTTCTGGAGTTACGGGAATGCGCCCAGATCTTATTATCAATCCACATGCATTTCCTTCAAGAATGACAATAGCCATGCTTTTGGAGTCGGTTGCTGCAAAGGGTGGTAGCTTACATGGAAAATATGTTGATGCGACACCATTTGCTAATTCAGAGCAGAAAGTTAATGAAGAAACTAATGGAGAGAAATCCAAAACCCTTGTTGATGAACTTGGTGAAATGTTAAAGGTTAAAGGGTTTAATTACCATGGTGCAGAGGTATTGTACAGTGGCGTCTATGGAACAGAACTCACGTGTGAAATCTTTATTGGTCCTGTTTACTATCAACGATTACGGCATATGGTTTCAGACAAATTTCAGGTTCGCTCTACGGGAATGGTAGATCAAGTCACACGCCAGCCTATCAAAGGACGAAAACGAGGTGGCGGTATACGTTTTGGTGAAATGGAGAGAGACTCCTTGCTTGCACATGGGGCAGCATATTTGTTGCATGATAGACTTCATACATGTTCTGATTATCACATTGCTGACGTATGTTCCCTTTGTGGATCCATCCTAACAACCTCATCAGTCCAGCCACAAATACGGGTTCGTGAAATGCGGGGCTTGCCCCCTATGAGAGCTCCCAAGAAGGTGACTTGCCATGCATGCAAGTCGAGTAAAGGGATGGAGACGGTTGCAATGCCTTATGTCTTTAGATATTTAGCAGCTGAGTTAGCAGCTATGAACATAAAACTGACCCTCCAGTTGAGCAATGCAACTGGAGCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000428 GO:0003674 GO:0003824 GO:0003899 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005736 GO:0005737 GO:0005829 GO:0006139 GO:0006351 GO:0006360 GO:0006725 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009553 GO:0009561 GO:0009987 GO:0010467 GO:0016070 GO:0016740 GO:0016772 GO:0016779 GO:0018130 GO:0019438 GO:0030880 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032774 GO:0032991 GO:0034062 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044452 GO:0044464 GO:0046483 GO:0048229 GO:0048856 GO:0055029 GO:0061695 GO:0070013 GO:0071704 GO:0090304 GO:0097659 GO:0097747 GO:0140098 GO:1901360 GO:1901362 GO:1901576 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko00230 ko00240 ko01100 ko03020 map00230 map00240 map01100 map03020

Pfam Domains

Protein Families

RNA_pol_Rpa2_4 RNA_pol_Rpb2_1 RNA_pol_Rpb2_2 RNA_pol_Rpb2_3 RNA_pol_Rpb2_6 RNA_pol_Rpb2_7

Protein Analysis

1137

Amino Acids

128.01

Weight (kDa)

8.83

Isoelectric Point (pI)

37.01

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RNA_pol_Rpb2_1	PF04563	38 - 368	1.7e-26	RNA polymerase beta subunit
RNA_pol_Rpb2_2	PF04561	161 - 333	5.1e-10	RNA polymerase Rpb2, domain 2
RNA_pol_Rpb2_3	PF04565	421 - 484	1.1e-27	RNA polymerase Rpb2, domain 3
RNA_pol_Rpa2_4	PF06883	538 - 595	3.6e-21	RNA polymerase I, Rpa2 specific domain
RNA_pol_Rpb2_6	PF00562	653 - 1021	1.7e-121	RNA polymerase Rpb2, domain 6
RNA_pol_Rpb2_7	PF04560	1023 - 1130	1e-21	RNA polymerase Rpb2, domain 7

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0005765)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G29940
fragaria_vesca	FvH4_5g25870 FvH4_5g25870 FvH4_7g07420 FvH4_7g07420 FvH4_7g07420 FvH4_7g07420 FvH4_7g07420 FvH4_7g07420 FvH4_7g07420
malus_domestica	MD08G1237100.v1.1 MD15G1428900.v1.1
prunus_persica	Prupe.1G574600_v2.0.a1 Prupe.1G574600_v2.0.a1 Prupe.7G107000_v2.0.a1 Prupe.7G184700_v2.0.a1 Prupe.7G223000_v2.0.a1
pyrus_communis	pycom08g20550
rosa_chinensis	RchiOBHm_Chr1g0339571 RchiOBHm_Chr1g0339591
rosa_laevigata	RLG00000029239
rosa_multiflora	Rmu_sc0015580.1_g000004
rosa_roxburghii	Rroxscaffold_4G00313560 Rroxscaffold_4G00313600
rosa_rugosa	Rorug01G0140100.1
rosa_samantha	Rh1AG158100 Rh1CG147600
rosa_wichuraiana	Rw1G013100

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	734
Acc16I	TGCGCA	1 cut(s)	431
Acc36I	ACCTGC	1 cut(s)	540
AccB1I	GGYRCC	3 cut(s)	60, 2447, 2550
AccB7I	CCANNNNNTGG	1 cut(s)	2648
AccI	GTMKAC	4 cut(s)	157, 2129, 2192, 3073
AccII	CGCG	1 cut(s)	48
AciI	CCGC	7 cut(s)	24, 406, 1244, 1580, 2116, 3069, 3244
AclWI	GGATC	7 cut(s)	89, 129, 296, 957, 1590, 3186, 3199
AcoI	YGGCCR	3 cut(s)	250, 1009, 1784
AcsI	RAATTY	8 cut(s)	168, 244, 636, 671, 902, 1929, 2579, 2996
AcuI	CTGAAG	4 cut(s)	492, 1668, 2111, 2510
AcvI	CACGTG	1 cut(s)	2937
AcyI	GRCGYC	1 cut(s)	2916
AfaI	GTAC	5 cut(s)	424, 1460, 2009, 2338, 2909
AfiI	CCNNNNNNNGG	8 cut(s)	104, 812, 1371, 2390, 2570, 2622, 2648, 3050
AflII	CTTAAG	2 cut(s)	34, 289
AflIII	ACRYGT	2 cut(s)	2936, 3149
AhdI	GACNNNNNGTC	1 cut(s)	2500
AhlI	ACTAGT	1 cut(s)	2263
AjnI	CCWGG	4 cut(s)	1006, 1370, 1744, 1973
AjuI	GAANNNNNNNTTGG	2 cut(s)	2934, 2966
AleI	CACNNNNGTG	1 cut(s)	1346
AloI	GAACNNNNNNTCC	2 cut(s)	840, 872
Alw21I	GWGCWC	2 cut(s)	798, 3268
Alw26I	GTCTC	4 cut(s)	24, 1754, 3090, 3308
AlwI	GGATC	7 cut(s)	89, 129, 296, 957, 1590, 3186, 3199
Ama87I	CYCGRG	1 cut(s)	371
AoxI	GGCC	6 cut(s)	106, 250, 1009, 1784, 1841, 2624
ApaI	GGGCCC	1 cut(s)	1845
ApoI	RAATTY	8 cut(s)	168, 244, 636, 671, 902, 1929, 2579, 2996
ArsI	GACNNNNNNTTYG	4 cut(s)	543, 575, 857, 889
AseI	ATTAAT	1 cut(s)	1209
Asp700I	GAANNNNTTC	2 cut(s)	578, 1929
AspLEI	GCGC	5 cut(s)	48, 432, 548, 724, 2667
AspS9I	GGNCC	5 cut(s)	1062, 1571, 1841, 1842, 2954
AsuC2I	CCSGG	1 cut(s)	2197
AsuHPI	GGTGA	7 cut(s)	725, 872, 2545, 2585, 2870, 3095, 3289
AsuII	TTCGAA	2 cut(s)	1493, 1501
AvaI	CYCGRG	1 cut(s)	371
AvaII	GGWCC	3 cut(s)	1062, 1571, 2954
BaeGI	GKGCMC	2 cut(s)	1845, 2165
BalI	TGGCCA	2 cut(s)	252, 1011
BamHI	GGATCC	1 cut(s)	3191
BanI	GGYRCC	3 cut(s)	60, 2447, 2550
BanII	GRGCYC	4 cut(s)	372, 1845, 1997, 3268
BarI	GAAGNNNNNNTAC	2 cut(s)	1195, 1227
BbrPI	CACGTG	1 cut(s)	2937
BbsI	GAAGAC	5 cut(s)	498, 749, 903, 2099, 2372
Bbv12I	GWGCWC	2 cut(s)	798, 3268
BbvCI	CCTCAGC	1 cut(s)	306
BceAI	ACGGC	2 cut(s)	839, 2993
BcgI	CGANNNNNNTGC	2 cut(s)	3279, 3313
BciT130I	CCWGG	4 cut(s)	1008, 1372, 1746, 1975
BciVI	GTATCC	2 cut(s)	558, 1706
BclI	TGATCA	1 cut(s)	499
BcnI	CCSGG	1 cut(s)	2197
BcoDI	GTCTC	4 cut(s)	24, 1754, 3090, 3308
BcuI	ACTAGT	1 cut(s)	2263
BfaI	CTAG	9 cut(s)	104, 314, 779, 884, 1043, 1226, 1773, 2264, 2556
BfmI	CTRYAG	2 cut(s)	693, 2238
BfrI	CTTAAG	2 cut(s)	34, 289
BfuAI	ACCTGC	1 cut(s)	540
BfuI	GTATCC	2 cut(s)	558, 1706
BglII	AGATCT	1 cut(s)	2671
Bme1390I	CCNGG	5 cut(s)	1008, 1372, 1746, 1975, 2197
Bme18I	GGWCC	3 cut(s)	1062, 1571, 2954
BmeRI	GACNNNNNGTC	1 cut(s)	2500
BmeT110I	CYCGRG	1 cut(s)	371
BmgT120I	GGNCC	5 cut(s)	1062, 1571, 1841, 1842, 2954
BmiI	GGNNCC	7 cut(s)	62, 225, 1843, 2449, 2481, 2552, 3193
BmrFI	CCNGG	5 cut(s)	1008, 1372, 1746, 1975, 2197
BmrI	ACTGGG	2 cut(s)	1950, 2611
BmsI	GCATC	7 cut(s)	841, 1456, 1894, 1935, 2203, 2530, 2764
BmuI	ACTGGG	2 cut(s)	1950, 2611
BoxI	GACNNNNGTC	1 cut(s)	753
BpiI	GAAGAC	5 cut(s)	498, 749, 903, 2099, 2372
BplI	GAGNNNNNCTC	2 cut(s)	3083, 3115
BpmI	CTGGAG	3 cut(s)	842, 2670, 3371
Bpu10I	CCTNAGC	1 cut(s)	306
Bpu14I	TTCGAA	2 cut(s)	1493, 1501
BpuEI	CTTGAG	2 cut(s)	845, 1016
BpuMI	CCSGG	1 cut(s)	2197
Bsa29I	ATCGAT	1 cut(s)	2367
BsaAI	YACGTR	1 cut(s)	2937
BsaBI	GATNNNNATC	2 cut(s)	1288, 2151
BsaHI	GRCGYC	1 cut(s)	2916
BsaI	GGTCTC	1 cut(s)	1754
BsaJI	CCNNGG	4 cut(s)	1006, 1371, 1973, 2890
BsaXI	ACNNNNNCTCC	4 cut(s)	840, 870, 3369, 3399
Bsc4I	CCNNNNNNNGG	8 cut(s)	104, 812, 1371, 2390, 2570, 2622, 2648, 3050
Bse1I	ACTGG	9 cut(s)	253, 825, 1204, 1787, 1945, 2011, 2617, 3388, 3409
Bse3DI	GCAATG	3 cut(s)	3165, 3330, 3403
Bse8I	GATNNNNATC	2 cut(s)	1288, 2151
BseBI	CCWGG	4 cut(s)	1008, 1372, 1746, 1975
BseCI	ATCGAT	1 cut(s)	2367
BseDI	CCNNGG	4 cut(s)	1006, 1371, 1973, 2890
BseGI	GGATG	6 cut(s)	1933, 1950, 2218, 2380, 3195, 3315
BseJI	GATNNNNATC	2 cut(s)	1288, 2151
BseLI	CCNNNNNNNGG	8 cut(s)	104, 812, 1371, 2390, 2570, 2622, 2648, 3050
BseMI	GCAATG	3 cut(s)	3165, 3330, 3403
BseMII	CTCAG	7 cut(s)	128, 320, 716, 1738, 1963, 2216, 3345
BseNI	ACTGG	9 cut(s)	253, 825, 1204, 1787, 1945, 2011, 2617, 3388, 3409
BseRI	GAGGAG	2 cut(s)	28, 2088
BseSI	GKGCMC	2 cut(s)	1845, 2165
BseYI	CCCAGC	1 cut(s)	1328
BsgI	GTGCAG	2 cut(s)	2088, 2916
Bsh1236I	CGCG	1 cut(s)	48
BshFI	GGCC	6 cut(s)	108, 252, 1011, 1786, 1843, 2626
BshNI	GGYRCC	3 cut(s)	60, 2447, 2550
BshVI	ATCGAT	1 cut(s)	2367
BsiHKAI	GWGCWC	2 cut(s)	798, 3268
BsiHKCI	CYCGRG	1 cut(s)	371
BsiSI	CCGG	3 cut(s)	27, 58, 2196
BslI	CCNNNNNNNGG	8 cut(s)	104, 812, 1371, 2390, 2570, 2622, 2648, 3050
BsmAI	GTCTC	4 cut(s)	24, 1754, 3090, 3308
BsmBI	CGTCTC	1 cut(s)	3308
BsmI	GAATGC	1 cut(s)	2667
BsnI	GGCC	6 cut(s)	108, 252, 1011, 1786, 1843, 2626
Bso31I	GGTCTC	1 cut(s)	1754
BsoBI	CYCGRG	1 cut(s)	371
Bsp119I	TTCGAA	2 cut(s)	1493, 1501
Bsp120I	GGGCCC	1 cut(s)	1841
Bsp1286I	GDGCHC	6 cut(s)	372, 798, 1845, 1997, 2165, 3268
Bsp1407I	TGTACA	1 cut(s)	2907
Bsp19I	CCATGG	1 cut(s)	2890
BspACI	CCGC	7 cut(s)	24, 406, 1244, 1580, 2116, 3069, 3244
BspANI	GGCC	6 cut(s)	108, 252, 1011, 1786, 1843, 2626
BspCNI	CTCAG	7 cut(s)	127, 319, 715, 1737, 1962, 2217, 3346
BspDI	ATCGAT	1 cut(s)	2367
BspFNI	CGCG	1 cut(s)	48
BspHI	TCATGA	2 cut(s)	298, 1921
BspLI	GGNNCC	7 cut(s)	62, 225, 1843, 2449, 2481, 2552, 3193
BspMI	ACCTGC	1 cut(s)	540
BspPI	GGATC	7 cut(s)	89, 129, 296, 957, 1590, 3186, 3199
BspT104I	TTCGAA	2 cut(s)	1493, 1501
BspT107I	GGYRCC	3 cut(s)	60, 2447, 2550
BspTI	CTTAAG	2 cut(s)	34, 289
BspTNI	GGTCTC	1 cut(s)	1754
BsrDI	GCAATG	3 cut(s)	3165, 3330, 3403
BsrGI	TGTACA	1 cut(s)	2907
BsrI	ACTGG	9 cut(s)	253, 825, 1204, 1787, 1945, 2011, 2617, 3388, 3409
BssECI	CCNNGG	4 cut(s)	1006, 1371, 1973, 2890
BssNAI	GTATAC	3 cut(s)	2130, 2193, 3074
BssNI	GRCGYC	1 cut(s)	2916
BssT1I	CCWWGG	1 cut(s)	2890
Bst1107I	GTATAC	3 cut(s)	2130, 2193, 3074
Bst2UI	CCWGG	4 cut(s)	1008, 1372, 1746, 1975
Bst4CI	ACNGT	6 cut(s)	427, 517, 1255, 2141, 2912, 3319
Bst6I	CTCTTC	2 cut(s)	5, 324
BstACI	GRCGYC	1 cut(s)	2916
BstAFI	CTTAAG	2 cut(s)	34, 289
BstAPI	GCANNNNNTGC	1 cut(s)	2177
BstAUI	TGTACA	1 cut(s)	2907
BstBAI	YACGTR	1 cut(s)	2937
BstBI	TTCGAA	2 cut(s)	1493, 1501
BstDEI	CTNAG	7 cut(s)	114, 306, 702, 1724, 1949, 2225, 3354
BstDSI	CCRYGG	1 cut(s)	2890
BstENI	CCTNNNNNAGG	2 cut(s)	102, 3048
BstF5I	GGATG	6 cut(s)	1933, 1950, 2218, 2380, 3195, 3315
BstFNI	CGCG	1 cut(s)	48
BstHHI	GCGC	5 cut(s)	48, 432, 548, 724, 2667
BstMAI	GTCTC	4 cut(s)	24, 1754, 3090, 3308
BstNI	CCWGG	4 cut(s)	1008, 1372, 1746, 1975
BstNSI	RCATGY	4 cut(s)	657, 2693, 3153, 3294
BstPAI	GACNNNNGTC	1 cut(s)	753
BstSCI	CCNGG	5 cut(s)	1006, 1370, 1744, 1973, 2195
BstSFI	CTRYAG	2 cut(s)	693, 2238
BstSLI	GKGCMC	2 cut(s)	1845, 2165
BstUI	CGCG	1 cut(s)	48
BstV2I	GAAGAC	5 cut(s)	498, 749, 903, 2099, 2372
BstX2I	RGATCY	3 cut(s)	1582, 2671, 3191
BstXI	CCANNNNNNTGG	1 cut(s)	2725
BstYI	RGATCY	3 cut(s)	1582, 2671, 3191
BstZ17I	GTATAC	3 cut(s)	2130, 2193, 3074
Bsu15I	ATCGAT	1 cut(s)	2367
BsuI	GTATCC	2 cut(s)	558, 1706
BsuRI	GGCC	6 cut(s)	108, 252, 1011, 1786, 1843, 2626
BsuTUI	ATCGAT	1 cut(s)	2367
BtgI	CCRYGG	1 cut(s)	2890
BtsCI	GGATG	6 cut(s)	1933, 1950, 2218, 2380, 3195, 3315
BtsI	GCAGTG	1 cut(s)	1547
BtsIMutI	CAGTG	5 cut(s)	1547, 1692, 1957, 2018, 2917
BveI	ACCTGC	1 cut(s)	540
CciI	TCATGA	2 cut(s)	298, 1921
CfoI	GCGC	5 cut(s)	48, 432, 548, 724, 2667
Cfr13I	GGNCC	5 cut(s)	1062, 1571, 1841, 1842, 2954
ClaI	ATCGAT	1 cut(s)	2367
CseI	GACGC	2 cut(s)	1111, 2905
Csp6I	GTAC	5 cut(s)	423, 1459, 2008, 2337, 2908
CspCI	CAANNNNNGTGG	2 cut(s)	688, 723
CviQI	GTAC	5 cut(s)	423, 1459, 2008, 2337, 2908
DdeI	CTNAG	7 cut(s)	114, 306, 702, 1724, 1949, 2225, 3354
DraI	TTTAAA	1 cut(s)	606
DriI	GACNNNNNGTC	1 cut(s)	2500
EaeI	YGGCCR	3 cut(s)	250, 1009, 1784
Eam1104I	CTCTTC	2 cut(s)	5, 324
Eam1105I	GACNNNNNGTC	1 cut(s)	2500
EarI	CTCTTC	2 cut(s)	5, 324
EciI	GGCGGA	1 cut(s)	13
Ecl136II	GAGCTC	1 cut(s)	3266
Eco130I	CCWWGG	1 cut(s)	2890
Eco24I	GRGCYC	4 cut(s)	372, 1845, 1997, 3268
Eco31I	GGTCTC	1 cut(s)	1754
Eco47I	GGWCC	3 cut(s)	1062, 1571, 2954
Eco53kI	GAGCTC	1 cut(s)	3266
Eco57I	CTGAAG	4 cut(s)	492, 1668, 2111, 2510
Eco72I	CACGTG	1 cut(s)	2937
Eco88I	CYCGRG	1 cut(s)	371
EcoICRI	GAGCTC	1 cut(s)	3266
EcoNI	CCTNNNNNAGG	2 cut(s)	102, 3048
EcoO109I	RGGNCCY	1 cut(s)	1842
EcoRII	CCWGG	4 cut(s)	1006, 1370, 1744, 1973
EcoT14I	CCWWGG	1 cut(s)	2890
EcoT22I	ATGCAT	2 cut(s)	2695, 3292
EcoT38I	GRGCYC	4 cut(s)	372, 1845, 1997, 3268
ErhI	CCWWGG	1 cut(s)	2890
Esp3I	CGTCTC	1 cut(s)	3308
FalI	AAGNNNNNCTT	2 cut(s)	566, 598
FauI	CCCGC	2 cut(s)	1237, 3237
FauNDI	CATATG	2 cut(s)	1431, 2982
FbaI	TGATCA	1 cut(s)	499
FblI	GTMKAC	4 cut(s)	157, 2129, 2192, 3073
FokI	GGATG	6 cut(s)	1920, 1957, 2225, 2387, 3182, 3322
FriOI	GRGCYC	4 cut(s)	372, 1845, 1997, 3268
FspBI	CTAG	9 cut(s)	104, 314, 779, 884, 1043, 1226, 1773, 2264, 2556
FspI	TGCGCA	1 cut(s)	431
GlaI	GCGC	5 cut(s)	47, 431, 547, 723, 2666
GsaI	CCCAGC	1 cut(s)	1332
GsuI	CTGGAG	3 cut(s)	842, 2670, 3371
HaeIII	GGCC	6 cut(s)	108, 252, 1011, 1786, 1843, 2626
HapII	CCGG	3 cut(s)	27, 58, 2196
HgaI	GACGC	2 cut(s)	1111, 2905
HhaI	GCGC	5 cut(s)	48, 432, 548, 724, 2667
Hin1I	GRCGYC	1 cut(s)	2916
Hin6I	GCGC	5 cut(s)	46, 430, 546, 722, 2665
HinP1I	GCGC	5 cut(s)	46, 430, 546, 722, 2665
HincII	GTYRAC	2 cut(s)	158, 2494
HindII	GTYRAC	2 cut(s)	158, 2494
HpaII	CCGG	3 cut(s)	27, 58, 2196
HphI	GGTGA	7 cut(s)	725, 872, 2545, 2585, 2870, 3095, 3289
Hpy166II	GTNNAC	9 cut(s)	158, 818, 880, 2130, 2193, 2494, 2536, 2962, 3074
Hpy8I	GTNNAC	9 cut(s)	158, 818, 880, 2130, 2193, 2494, 2536, 2962, 3074
Hpy99I	CGWCG	1 cut(s)	1127
HpyCH4III	ACNGT	6 cut(s)	427, 517, 1255, 2141, 2912, 3319
HpyCH4IV	ACGT	5 cut(s)	1716, 2126, 2936, 3076, 3174
HpyF3I	CTNAG	7 cut(s)	114, 306, 702, 1724, 1949, 2225, 3354
HpySE526I	ACGT	5 cut(s)	1716, 2126, 2936, 3076, 3174
Hsp92I	GRCGYC	1 cut(s)	2916
HspAI	GCGC	5 cut(s)	46, 430, 546, 722, 2665
Ksp22I	TGATCA	1 cut(s)	499
LmnI	GCTCC	5 cut(s)	229, 2479, 2618, 3271, 3407
LweI	GCATC	7 cut(s)	841, 1456, 1894, 1935, 2203, 2530, 2764
MaeI	CTAG	9 cut(s)	104, 314, 779, 884, 1043, 1226, 1773, 2264, 2556
MaeII	ACGT	5 cut(s)	1716, 2126, 2936, 3076, 3174
MaeIII	GTNAC	5 cut(s)	511, 2349, 2653, 3031, 3277
MflI	RGATCY	3 cut(s)	1582, 2671, 3191
MhlI	GDGCHC	6 cut(s)	372, 798, 1845, 1997, 2165, 3268
MlsI	TGGCCA	2 cut(s)	252, 1011
MluNI	TGGCCA	2 cut(s)	252, 1011
MlyI	GAGTC	6 cut(s)	157, 341, 1429, 2089, 2738, 3092
MmeI	TCCRAC	5 cut(s)	826, 1513, 1961, 2180, 2457
Mox20I	TGGCCA	2 cut(s)	252, 1011
Mph1103I	ATGCAT	2 cut(s)	2695, 3292
MroXI	GAANNNNTTC	2 cut(s)	578, 1929
MscI	TGGCCA	2 cut(s)	252, 1011
MslI	CAYNNNNRTG	5 cut(s)	269, 920, 1299, 1346, 2636
Msp20I	TGGCCA	2 cut(s)	252, 1011
MspA1I	CMGCKG	2 cut(s)	1679, 3353
MspCI	CTTAAG	2 cut(s)	34, 289
MspI	CCGG	3 cut(s)	27, 58, 2196
MspR9I	CCNGG	5 cut(s)	1008, 1372, 1746, 1975, 2197
Mva1269I	GAATGC	1 cut(s)	2667
MvaI	CCWGG	4 cut(s)	1008, 1372, 1746, 1975
MvnI	CGCG	1 cut(s)	48
NciI	CCSGG	1 cut(s)	2197
NcoI	CCATGG	1 cut(s)	2890
NdeI	CATATG	2 cut(s)	1431, 2982
NlaIV	GGNNCC	7 cut(s)	62, 225, 1843, 2449, 2481, 2552, 3193
NmuCI	GTSAC	3 cut(s)	2349, 3031, 3277
NsbI	TGCGCA	1 cut(s)	431
NsiI	ATGCAT	2 cut(s)	2695, 3292
NspI	RCATGY	4 cut(s)	657, 2693, 3153, 3294
NspV	TTCGAA	2 cut(s)	1493, 1501
OliI	CACNNNNGTG	1 cut(s)	1346
PaeI	GCATGC	1 cut(s)	3294
PaeR7I	CTCGAG	1 cut(s)	371
PagI	TCATGA	2 cut(s)	298, 1921
PciI	ACATGT	1 cut(s)	3149
PcsI	WCGNNNNNNNCGW	1 cut(s)	163
PctI	GAATGC	1 cut(s)	2667
PdmI	GAANNNNTTC	2 cut(s)	578, 1929
PfeI	GAWTC	8 cut(s)	977, 1367, 1498, 1690, 1804, 2221, 2279, 2369
PflMI	CCANNNNNTGG	1 cut(s)	2648
PleI	GAGTC	6 cut(s)	157, 340, 1429, 2089, 2737, 3092
PmaCI	CACGTG	1 cut(s)	2937
PmlI	CACGTG	1 cut(s)	2937
PpsI	GAGTC	6 cut(s)	157, 340, 1429, 2089, 2737, 3092
Ppu21I	YACGTR	1 cut(s)	2937
PscI	ACATGT	1 cut(s)	3149
PshAI	GACNNNNGTC	1 cut(s)	753
PshBI	ATTAAT	1 cut(s)	1209
PsiI	TTATAA	1 cut(s)	734
Psp124BI	GAGCTC	1 cut(s)	3268
Psp6I	CCWGG	4 cut(s)	1006, 1370, 1744, 1973
PspCI	CACGTG	1 cut(s)	2937
PspFI	CCCAGC	1 cut(s)	1328
PspGI	CCWGG	4 cut(s)	1006, 1370, 1744, 1973
PspN4I	GGNNCC	7 cut(s)	62, 225, 1843, 2449, 2481, 2552, 3193
PspOMI	GGGCCC	1 cut(s)	1841
PspPI	GGNCC	5 cut(s)	1062, 1571, 1841, 1842, 2954
PsuI	RGATCY	3 cut(s)	1582, 2671, 3191
PvuII	CAGCTG	2 cut(s)	1679, 3353
RsaI	GTAC	5 cut(s)	424, 1460, 2009, 2338, 2909
RsaNI	GTAC	5 cut(s)	423, 1459, 2008, 2337, 2908
RseI	CAYNNNNRTG	5 cut(s)	269, 920, 1299, 1346, 2636
SacI	GAGCTC	1 cut(s)	3268
SalI	GTCGAC	1 cut(s)	156
Sau96I	GGNCC	5 cut(s)	1062, 1571, 1841, 1842, 2954
SchI	GAGTC	6 cut(s)	157, 341, 1429, 2089, 2738, 3092
ScrFI	CCNGG	5 cut(s)	1008, 1372, 1746, 1975, 2197
SduI	GDGCHC	6 cut(s)	372, 798, 1845, 1997, 2165, 3268
SfaNI	GCATC	7 cut(s)	841, 1456, 1894, 1935, 2203, 2530, 2764
SfcI	CTRYAG	2 cut(s)	693, 2238
Sfr274I	CTCGAG	1 cut(s)	371
SfuI	TTCGAA	2 cut(s)	1493, 1501
SinI	GGWCC	3 cut(s)	1062, 1571, 2954
SlaI	CTCGAG	1 cut(s)	371
SmiMI	CAYNNNNRTG	5 cut(s)	269, 920, 1299, 1346, 2636
SmlI	CTYRAG	5 cut(s)	34, 289, 371, 860, 1031
SmoI	CTYRAG	5 cut(s)	34, 289, 371, 860, 1031
SpeI	ACTAGT	1 cut(s)	2263
SphI	GCATGC	1 cut(s)	3294
SsiI	CCGC	7 cut(s)	24, 406, 1244, 1580, 2116, 3069, 3244
SspI	AATATT	2 cut(s)	1480, 1798
SspMI	CTAG	9 cut(s)	104, 314, 779, 884, 1043, 1226, 1773, 2264, 2556
SstI	GAGCTC	1 cut(s)	3268
StyD4I	CCNGG	5 cut(s)	1006, 1370, 1744, 1973, 2195
StyI	CCWWGG	1 cut(s)	2890
TaaI	ACNGT	6 cut(s)	427, 517, 1255, 2141, 2912, 3319
TaiI	ACGT	5 cut(s)	1719, 2129, 2939, 3079, 3177
TatI	WGTACW	2 cut(s)	2336, 2907
TfiI	GAWTC	8 cut(s)	977, 1367, 1498, 1690, 1804, 2221, 2279, 2369
TscAI	CASTG	5 cut(s)	1554, 1692, 1957, 2018, 2917
TseFI	GTSAC	3 cut(s)	2349, 3031, 3277
Tsp45I	GTSAC	3 cut(s)	2349, 3031, 3277
TspGWI	ACGGA	2 cut(s)	555, 2291
TspRI	CASTG	5 cut(s)	1554, 1692, 1957, 2018, 2917
Van91I	CCANNNNNTGG	1 cut(s)	2648
Vha464I	CTTAAG	2 cut(s)	34, 289
VpaK11BI	GGWCC	3 cut(s)	1062, 1571, 2954
VspI	ATTAAT	1 cut(s)	1209
XagI	CCTNNNNNAGG	2 cut(s)	102, 3048
XapI	RAATTY	8 cut(s)	168, 244, 636, 671, 902, 1929, 2579, 2996
XbaI	TCTAGA	3 cut(s)	778, 1225, 1772
XceI	RCATGY	4 cut(s)	657, 2693, 3153, 3294
XcmI	CCANNNNNNNNNTGG	1 cut(s)	706
XhoI	CTCGAG	1 cut(s)	371
XmiI	GTMKAC	4 cut(s)	157, 2129, 2192, 3073
XmnI	GAANNNNTTC	2 cut(s)	578, 1929
XspI	CTAG	9 cut(s)	104, 314, 779, 884, 1043, 1226, 1773, 2264, 2556
Zsp2I	ATGCAT	2 cut(s)	2695, 3292

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.