Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr1

Orthogroup ID

OG0005765

Physical Location & Seq

Genomic Coordinates Forward (+)

29266135 .. 29281811

Gene Structure 15677 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw1G013100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3480 bp

ATGCAGCAGAAGAGGAGAACCTCCGCCGGAGACTTAAGCCACTTGCGGGAGCTGTTCCGGCACCACATAGAGTCCTTCGATAGTTCGACTACCTCATCACCGCCGGTCTCGGCGTCACGCTCCGTGACATCAAGCCCTATATTACTCTGGTTCGCTGATGATCCTATTCTAGGCCGACCTCAGAAGGATCGGAGTAGCGAGCCTCTCTATCCTTTTGAATGTCGACAGACTCGAATTTCCTACACGGGTAAATTGCTGGCAGACATTTACTTTCAGCGTGATGATAAGGCTCCTGTCAGAGAGCAGTTAAATTTTGGCCAGTTTCCCATCATGCTCATGTCAAAGTGTTGCAACTTAAGAGGTCATGATCCTCAGCAGCTAGTTTCTTGCAAAGAAGAGTCATCAGAAATGGGTGGCTACTTCATTTTGAATGGGCTCGAGAGAGTTATTCGACCTGTTATATTGCCGAAGCGGAACTATCCAATGAGTACGGTGCGCAATTCATTTCGTGATCGAAAGGAAGGATATACAGATAAAGCAGTTGTGATAAGATGTGTAAGAGATGATCAGTCATCAGTAACAGTCAAATTGTATTACCTGCAAAACGGAAGTGCAAGACTTGGTGTTTGGATACAGGGGAAGGAGTTCTTGCTTCCTGTGGGTCTTATTTTAAAGGCTTTTATTGACACTCCTGATCGTGAAATTTTTGCGAGTTTGACATGCTGTTGTAACGAGAAATTTGAGAAAGACAAAGGTGCTGTTGCCACTCAGCTTGTGGGTGAGAGGGCGCAAATTATTATAAATGAAGTGGCAGACTTGGGTCTTCACACTCATCTTCAATGTCTAGAACACATTGGCGAGCACTTTCAACCTCTAATGGGTGGACTGGAGAATGAACCTTATTCAGTTGTTGGAGATGCGGTTCTCAAGGACTACATATTTGTTCACCTAGATAAGAATGAAGACAAATTCAATCTGCTCATATTCATGTTGCAGAAACTTTTTTCGCTTATGGATCAGACTTCGGTTCTCGACAACCCAGATTCCTTGCAAAATCAAGAAGTCTTACTCCCTGGCCATCTGATAACCATATATCTCAAGGAAAAATTAGAGGATTGGTTGCAAAGGACCAAAAAACTTCTTCAAGATGAGATGAAAAAGGAAAGCAACAATATCAATTTTGGCAGCGTCGATAGCTTAAAAAAGGTTCTGGAAAAAAATCCTACGAGGCAAATCAGTATGGCGATTGAGAATTTGTTGAAAACTGGAAGAATTAATACTCAAACAGGTCTAGATTTGCAGCAGAGGGCGGGATTTACAGTTCAGGCAGAAAGGCTTAACTTTTTACGATTTTTATCGCATTTTCGTGCTGTTCATCGAGGGGCTTCATTTGCTGGGCTTCGCACCACAAGTGTGCGAAAATTGCTTCCCGAATCCTGGGGTTTCCTTTGCCCTGTGCATACACCTGATGGAGAGCCATGTGGCTTGTTGAACCATATGACTCGCACTTGTAGAATTACATCGTACTTTGATGCACAAGGGAATATTAGAGATTTCTCTAAGATTCGAATGTCTATTCTTAATGTTCTGATCGAAGTTGGAATGACACCTTCACTGCCAATGTTGGTTCATGCCGGTCCTCCTGCGGATCTTTCTGTTCTTTTAGATGGTCGTCTTGTTGGTTATATATCTTCCAAACAAGTTGAGAGAGTTGTGGCTCATTTAAGGAAGCTGAAAGTTTCAGCTGCTTCAGTGATTCCAGATGACTTGGAAGTGGGATACATTCCTCTCAGCATGGGTGGGGCATATCCTGGTCTCTACCTTAACACTTCTCCTTCTAGATTTGTTCGGCCAGTTAAGAATATTTCGATTCCTGCTGTTGAAGGTCAAAATATTGAACTCATTGGGCCCTTTGAACAGGTGTTCATGGAAATAAGATGTCCTGATGGTCGAGATGGTGGAAGGAAAGATGCTTTCCCTGCAACTCATGAAGAAATTCATCCAACTGGGATGCTCAGTGTTGTTGCTAATCTTACTCCCTGGTCAGAACATAATCAGAGCCCACGAAATATGTACCAGTGTCAGATGGCAAAACAAACGATGGGTTTCTCATTACAAGCACTTTGCAATCGTGCAGATCAGAAGTTGTATCATCTGAAGACTCCTCAATCTCCCATCGTCCGCACAAAAACGTATACGAATTACTGTGTAGATGAATATCCAACGGGCACAAATGCAATCGTTGCTGTATTGTCGTATACCGGGTACGATATGGAGGATGCTATGATTCTGAGCAAGTCATCTGTAGATCGTGGAATGTTTCATGGACTAGTATATCAGACGGAATCCATTGACTTGTCTGATACAAAGGGTAGTAAATCCCAGAGATCGTTCAGGAGAAGTACCACTGATAAGTCACATCATTCTTTGATCGATTCAGATGGTCTTCCTTATGTGGGTCAGACAATAAAACCAGATGAACCCTATTATAGCACTTATGATGAGATAAAAGGTGCCATAAGAACGACCAAACGAAAGGGGTCAGAGCCTGCTATTGTTGACTATGTTGCTATTGATGGGAAGAAGCATCTTCAGAAGGTGAACATAAGGTTTCGGCACCCTAGAAACCCGATAATTGGTGATAAATTTAGCAGCCGACATGGACAAAAAGGAGTTTGCTCCCAGTTGTGGCCTGACATTGATATGCCATTTTCTGGAGTTACGGGAATGCGACCAGATCTTATTATCAATCCACATGCATTTCCTTCAAGAATGACAATAGCCATGCTTTTGGAGTCGGTTGCTGCAAAGGGTGGTAGCTTACATGGAAAATATGTTGATGCGACACCATTTGCTAATTCAGAGGAGAAAGTTAATGAAGAAACTAATGGAGAGAAATCCAAAACCCTTGTTGATGAACTTGGTGAAATGTTAAAGGTTAAAGGGTTTAATTACCATGGTGCAGAGGTATTGTACAGTGGCGTCTATGGAACAGAACTCACGTGTGAAATCTTTATTGGTCCTGTTTACTATCAACGATTACGGCATATGGTTTCAGACAAATTTCAGGTTCGCTCTACGGGAATGGTAGATCAAGTCACACGCCAGCCTATCAAAGGACGAAAACGAGGTGGCGGTATACGTTTTGGTGAAATGGAGAGAGACTCCTTGCTTGCACATGGGGCAGCATATTTGTTGCATGATAGACTTCATACATGTTCTGATTATCACATTGCTGACGTATGTTCCCTTTGTGGATCCATCCTAACAACCTCATCAGTCCAGCCACAAATACGGGTTCGTGAAATGCGGGGCTTGCCCCCTATGAGAGCTCCCAAGAAGGTGACTTGCCATGCATGTAAGTCGAGTAAAGGGATGGAGACGGTTGCAATGCCTTATGTCTTTAGATATTTAGCAGCTGAGTTAGCAGCTATGAACATAAAACTGACCCTCCAGTTGAGCAATGCAACTGGAGCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000428 GO:0003674 GO:0003824 GO:0003899 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005736 GO:0005737 GO:0005829 GO:0006139 GO:0006351 GO:0006360 GO:0006725 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009553 GO:0009561 GO:0009987 GO:0010467 GO:0016070 GO:0016740 GO:0016772 GO:0016779 GO:0018130 GO:0019438 GO:0030880 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032774 GO:0032991 GO:0034062 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044452 GO:0044464 GO:0046483 GO:0048229 GO:0048856 GO:0055029 GO:0061695 GO:0070013 GO:0071704 GO:0090304 GO:0097659 GO:0097747 GO:0140098 GO:1901360 GO:1901362 GO:1901576 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko00230 ko00240 ko01100 ko03020 map00230 map00240 map01100 map03020

Pfam Domains

Protein Families

RNA_pol_Rpa2_4 RNA_pol_Rpb2_1 RNA_pol_Rpb2_2 RNA_pol_Rpb2_3 RNA_pol_Rpb2_6 RNA_pol_Rpb2_7

Protein Analysis

1159

Amino Acids

130.12

Weight (kDa)

8.75

Isoelectric Point (pI)

39.2

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RNA_pol_Rpb2_1	PF04563	61 - 390	9e-27	RNA polymerase beta subunit
RNA_pol_Rpb2_2	PF04561	183 - 355	3.9e-10	RNA polymerase Rpb2, domain 2
RNA_pol_Rpb2_3	PF04565	443 - 506	1.1e-27	RNA polymerase Rpb2, domain 3
RNA_pol_Rpa2_4	PF06883	560 - 617	5.2e-21	RNA polymerase I, Rpa2 specific domain
RNA_pol_Rpb2_6	PF00562	675 - 1043	1.9e-121	RNA polymerase Rpb2, domain 6
RNA_pol_Rpb2_7	PF04560	1045 - 1152	1e-21	RNA polymerase Rpb2, domain 7

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0005765)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G29940
fragaria_vesca	FvH4_5g25870 FvH4_5g25870 FvH4_7g07420 FvH4_7g07420 FvH4_7g07420 FvH4_7g07420 FvH4_7g07420 FvH4_7g07420 FvH4_7g07420
malus_domestica	MD08G1237100.v1.1 MD15G1428900.v1.1
prunus_persica	Prupe.1G574600_v2.0.a1 Prupe.1G574600_v2.0.a1 Prupe.7G107000_v2.0.a1 Prupe.7G184700_v2.0.a1 Prupe.7G223000_v2.0.a1
pyrus_communis	pycom08g20550
rosa_chinensis	RchiOBHm_Chr1g0339571 RchiOBHm_Chr1g0339591
rosa_laevigata	RLG00000029239
rosa_multiflora	Rmu_sc0015580.1_g000004
rosa_roxburghii	Rroxscaffold_4G00313560 Rroxscaffold_4G00313600
rosa_rugosa	Rorug01G0140100.1
rosa_samantha	Rh1AG158100 Rh1CG147600
rosa_wichuraiana	Rw1G013100

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	800
Acc16I	TGCGCA	1 cut(s)	497
Acc36I	ACCTGC	1 cut(s)	606
AccB1I	GGYRCC	3 cut(s)	60, 2513, 2616
AccB7I	CCANNNNNTGG	1 cut(s)	2714
AccI	GTMKAC	4 cut(s)	223, 2195, 2258, 3139
AclWI	GGATC	7 cut(s)	155, 195, 362, 1023, 1656, 3252, 3265
AcoI	YGGCCR	3 cut(s)	316, 1075, 1850
AcsI	RAATTY	9 cut(s)	234, 310, 702, 737, 968, 1252, 1995, 2645, 3062
AcuI	CTGAAG	3 cut(s)	1734, 2177, 2576
AcvI	CACGTG	1 cut(s)	3003
AcyI	GRCGYC	2 cut(s)	113, 2982
AfaI	GTAC	6 cut(s)	490, 1526, 2075, 2267, 2404, 2975
AfiI	CCNNNNNNNGG	9 cut(s)	46, 170, 878, 1437, 2456, 2636, 2688, 2714, 3116
AflII	CTTAAG	2 cut(s)	34, 355
AflIII	ACRYGT	2 cut(s)	3002, 3215
AhlI	ACTAGT	1 cut(s)	2329
AjnI	CCWGG	4 cut(s)	1072, 1436, 1810, 2039
AjuI	GAANNNNNNNTTGG	2 cut(s)	3000, 3032
AleI	CACNNNNGTG	1 cut(s)	1412
AloI	GAACNNNNNNTCC	4 cut(s)	906, 938, 1305, 1337
Alw21I	GWGCWC	2 cut(s)	864, 3334
Alw26I	GTCTC	5 cut(s)	24, 112, 1820, 3156, 3374
AlwI	GGATC	7 cut(s)	155, 195, 362, 1023, 1656, 3252, 3265
AlwNI	CAGNNNCTG	1 cut(s)	2549
Ama87I	CYCGRG	1 cut(s)	437
AoxI	GGCC	6 cut(s)	172, 316, 1075, 1850, 1907, 2690
ApaI	GGGCCC	1 cut(s)	1911
ApoI	RAATTY	9 cut(s)	234, 310, 702, 737, 968, 1252, 1995, 2645, 3062
ArsI	GACNNNNNNTTYG	4 cut(s)	609, 641, 923, 955
AseI	ATTAAT	1 cut(s)	1275
Asp700I	GAANNNNTTC	2 cut(s)	644, 1995
AspLEI	GCGC	2 cut(s)	498, 790
AspS9I	GGNCC	5 cut(s)	1128, 1637, 1907, 1908, 3020
AsuC2I	CCSGG	1 cut(s)	2263
AsuHPI	GGTGA	8 cut(s)	90, 791, 938, 2611, 2651, 2936, 3161, 3355
AsuII	TTCGAA	1 cut(s)	1567
AvaI	CYCGRG	1 cut(s)	437
AvaII	GGWCC	3 cut(s)	1128, 1637, 3020
BaeGI	GKGCMC	2 cut(s)	1911, 2231
BalI	TGGCCA	2 cut(s)	318, 1077
BamHI	GGATCC	1 cut(s)	3257
BanI	GGYRCC	3 cut(s)	60, 2513, 2616
BanII	GRGCYC	4 cut(s)	438, 1911, 2063, 3334
BarI	GAAGNNNNNNTAC	2 cut(s)	1261, 1293
BbrPI	CACGTG	1 cut(s)	3003
BbsI	GAAGAC	4 cut(s)	815, 969, 2165, 2438
Bbv12I	GWGCWC	2 cut(s)	864, 3334
BbvCI	CCTCAGC	1 cut(s)	372
BceAI	ACGGC	1 cut(s)	3059
BcgI	CGANNNNNNTGC	2 cut(s)	3345, 3379
BciT130I	CCWGG	4 cut(s)	1074, 1438, 1812, 2041
BciVI	GTATCC	2 cut(s)	624, 1772
BclI	TGATCA	1 cut(s)	565
BcnI	CCSGG	1 cut(s)	2263
BcoDI	GTCTC	5 cut(s)	24, 112, 1820, 3156, 3374
BcuI	ACTAGT	1 cut(s)	2329
BfaI	CTAG	8 cut(s)	170, 380, 845, 950, 1292, 1839, 2330, 2622
BfmI	CTRYAG	1 cut(s)	2304
BfrI	CTTAAG	2 cut(s)	34, 355
BfuAI	ACCTGC	1 cut(s)	606
BfuI	GTATCC	2 cut(s)	624, 1772
BglII	AGATCT	1 cut(s)	2737
Bme1390I	CCNGG	5 cut(s)	1074, 1438, 1812, 2041, 2263
Bme18I	GGWCC	3 cut(s)	1128, 1637, 3020
BmeT110I	CYCGRG	1 cut(s)	437
BmgT120I	GGNCC	5 cut(s)	1128, 1637, 1907, 1908, 3020
BmiI	GGNNCC	6 cut(s)	62, 291, 1909, 2515, 2618, 3259
BmrFI	CCNGG	5 cut(s)	1074, 1438, 1812, 2041, 2263
BmrI	ACTGGG	2 cut(s)	2016, 2677
BmsI	GCATC	7 cut(s)	907, 1522, 1960, 2001, 2269, 2596, 2830
BmuI	ACTGGG	2 cut(s)	2016, 2677
BoxI	GACNNNNGTC	1 cut(s)	819
BpiI	GAAGAC	4 cut(s)	815, 969, 2165, 2438
BplI	GAGNNNNNCTC	2 cut(s)	3149, 3181
BpmI	CTGGAG	3 cut(s)	908, 2736, 3437
Bpu10I	CCTNAGC	1 cut(s)	372
Bpu14I	TTCGAA	1 cut(s)	1567
BpuEI	CTTGAG	2 cut(s)	911, 1082
BpuMI	CCSGG	1 cut(s)	2263
Bsa29I	ATCGAT	1 cut(s)	2433
BsaAI	YACGTR	1 cut(s)	3003
BsaBI	GATNNNNATC	2 cut(s)	1354, 2217
BsaHI	GRCGYC	2 cut(s)	113, 2982
BsaI	GGTCTC	2 cut(s)	112, 1820
BsaJI	CCNNGG	4 cut(s)	1072, 1437, 2039, 2956
BsaXI	ACNNNNNCTCC	4 cut(s)	906, 936, 3435, 3465
Bsc4I	CCNNNNNNNGG	9 cut(s)	46, 170, 878, 1437, 2456, 2636, 2688, 2714, 3116
Bse118I	RCCGGY	2 cut(s)	103, 1634
Bse1I	ACTGG	9 cut(s)	319, 891, 1270, 1853, 2011, 2077, 2683, 3454, 3475
Bse3DI	GCAATG	3 cut(s)	3231, 3396, 3469
Bse8I	GATNNNNATC	2 cut(s)	1354, 2217
BseBI	CCWGG	4 cut(s)	1074, 1438, 1812, 2041
BseCI	ATCGAT	1 cut(s)	2433
BseDI	CCNNGG	4 cut(s)	1072, 1437, 2039, 2956
BseGI	GGATG	5 cut(s)	1999, 2016, 2284, 3261, 3381
BseJI	GATNNNNATC	2 cut(s)	1354, 2217
BseLI	CCNNNNNNNGG	9 cut(s)	46, 170, 878, 1437, 2456, 2636, 2688, 2714, 3116
BseMI	GCAATG	3 cut(s)	3231, 3396, 3469
BseMII	CTCAG	7 cut(s)	194, 386, 782, 1804, 2029, 2282, 3411
BseNI	ACTGG	9 cut(s)	319, 891, 1270, 1853, 2011, 2077, 2683, 3454, 3475
BseRI	GAGGAG	3 cut(s)	28, 2154, 2879
BseSI	GKGCMC	2 cut(s)	1911, 2231
BseYI	CCCAGC	1 cut(s)	1394
BsgI	GTGCAG	2 cut(s)	2154, 2982
BshFI	GGCC	6 cut(s)	174, 318, 1077, 1852, 1909, 2692
BshNI	GGYRCC	3 cut(s)	60, 2513, 2616
BshVI	ATCGAT	1 cut(s)	2433
BsiHKAI	GWGCWC	2 cut(s)	864, 3334
BsiHKCI	CYCGRG	1 cut(s)	437
BsiSI	CCGG	5 cut(s)	27, 58, 104, 1635, 2262
BslI	CCNNNNNNNGG	9 cut(s)	46, 170, 878, 1437, 2456, 2636, 2688, 2714, 3116
BsmAI	GTCTC	5 cut(s)	24, 112, 1820, 3156, 3374
BsmBI	CGTCTC	1 cut(s)	3374
BsmI	GAATGC	1 cut(s)	2733
BsnI	GGCC	6 cut(s)	174, 318, 1077, 1852, 1909, 2692
Bso31I	GGTCTC	2 cut(s)	112, 1820
BsoBI	CYCGRG	1 cut(s)	437
Bsp119I	TTCGAA	1 cut(s)	1567
Bsp120I	GGGCCC	1 cut(s)	1907
Bsp1286I	GDGCHC	6 cut(s)	438, 864, 1911, 2063, 2231, 3334
Bsp1407I	TGTACA	1 cut(s)	2973
Bsp19I	CCATGG	1 cut(s)	2956
BspANI	GGCC	6 cut(s)	174, 318, 1077, 1852, 1909, 2692
BspCNI	CTCAG	7 cut(s)	193, 385, 781, 1803, 2028, 2283, 3412
BspDI	ATCGAT	1 cut(s)	2433
BspHI	TCATGA	2 cut(s)	364, 1987
BspLI	GGNNCC	6 cut(s)	62, 291, 1909, 2515, 2618, 3259
BspMI	ACCTGC	1 cut(s)	606
BspPI	GGATC	7 cut(s)	155, 195, 362, 1023, 1656, 3252, 3265
BspT104I	TTCGAA	1 cut(s)	1567
BspT107I	GGYRCC	3 cut(s)	60, 2513, 2616
BspTI	CTTAAG	2 cut(s)	34, 355
BspTNI	GGTCTC	2 cut(s)	112, 1820
BsrDI	GCAATG	3 cut(s)	3231, 3396, 3469
BsrFI	RCCGGY	2 cut(s)	103, 1634
BsrGI	TGTACA	1 cut(s)	2973
BsrI	ACTGG	9 cut(s)	319, 891, 1270, 1853, 2011, 2077, 2683, 3454, 3475
BssAI	RCCGGY	2 cut(s)	103, 1634
BssECI	CCNNGG	4 cut(s)	1072, 1437, 2039, 2956
BssNAI	GTATAC	3 cut(s)	2196, 2259, 3140
BssNI	GRCGYC	2 cut(s)	113, 2982
BssT1I	CCWWGG	1 cut(s)	2956
Bst1107I	GTATAC	3 cut(s)	2196, 2259, 3140
Bst2UI	CCWGG	4 cut(s)	1074, 1438, 1812, 2041
Bst4CI	ACNGT	6 cut(s)	493, 583, 1321, 2207, 2978, 3385
Bst6I	CTCTTC	2 cut(s)	5, 390
BstACI	GRCGYC	2 cut(s)	113, 2982
BstAFI	CTTAAG	2 cut(s)	34, 355
BstAPI	GCANNNNNTGC	1 cut(s)	2243
BstAUI	TGTACA	1 cut(s)	2973
BstBAI	YACGTR	1 cut(s)	3003
BstBI	TTCGAA	1 cut(s)	1567
BstC8I	GCNNGC	7 cut(s)	200, 258, 860, 2550, 3107, 3174, 3317
BstDEI	CTNAG	8 cut(s)	180, 372, 768, 1560, 1790, 2015, 2291, 3420
BstDSI	CCRYGG	1 cut(s)	2956
BstENI	CCTNNNNNAGG	2 cut(s)	168, 3114
BstF5I	GGATG	5 cut(s)	1999, 2016, 2284, 3261, 3381
BstHHI	GCGC	2 cut(s)	498, 790
BstMAI	GTCTC	5 cut(s)	24, 112, 1820, 3156, 3374
BstNI	CCWGG	4 cut(s)	1074, 1438, 1812, 2041
BstNSI	RCATGY	4 cut(s)	723, 2759, 3219, 3360
BstPAI	GACNNNNGTC	1 cut(s)	819
BstSCI	CCNGG	5 cut(s)	1072, 1436, 1810, 2039, 2261
BstSFI	CTRYAG	1 cut(s)	2304
BstSLI	GKGCMC	2 cut(s)	1911, 2231
BstV2I	GAAGAC	4 cut(s)	815, 969, 2165, 2438
BstX2I	RGATCY	3 cut(s)	1648, 2737, 3257
BstXI	CCANNNNNNTGG	1 cut(s)	2791
BstYI	RGATCY	3 cut(s)	1648, 2737, 3257
BstZ17I	GTATAC	3 cut(s)	2196, 2259, 3140
Bsu15I	ATCGAT	1 cut(s)	2433
BsuI	GTATCC	2 cut(s)	624, 1772
BsuRI	GGCC	6 cut(s)	174, 318, 1077, 1852, 1909, 2692
BsuTUI	ATCGAT	1 cut(s)	2433
BtgI	CCRYGG	1 cut(s)	2956
BtsCI	GGATG	5 cut(s)	1999, 2016, 2284, 3261, 3381
BtsI	GCAGTG	1 cut(s)	1613
BtsIMutI	CAGTG	6 cut(s)	1613, 1758, 2023, 2084, 2406, 2983
BveI	ACCTGC	1 cut(s)	606
Cac8I	GCNNGC	7 cut(s)	200, 258, 860, 2550, 3107, 3174, 3317
CaiI	CAGNNNCTG	1 cut(s)	2549
CciI	TCATGA	2 cut(s)	364, 1987
CfoI	GCGC	2 cut(s)	498, 790
Cfr10I	RCCGGY	2 cut(s)	103, 1634
Cfr13I	GGNCC	5 cut(s)	1128, 1637, 1907, 1908, 3020
ClaI	ATCGAT	1 cut(s)	2433
CseI	GACGC	3 cut(s)	102, 1177, 2971
Csp6I	GTAC	6 cut(s)	489, 1525, 2074, 2266, 2403, 2974
CspCI	CAANNNNNGTGG	2 cut(s)	754, 789
CviQI	GTAC	6 cut(s)	489, 1525, 2074, 2266, 2403, 2974
DdeI	CTNAG	8 cut(s)	180, 372, 768, 1560, 1790, 2015, 2291, 3420
DraI	TTTAAA	1 cut(s)	672
EaeI	YGGCCR	3 cut(s)	316, 1075, 1850
Eam1104I	CTCTTC	2 cut(s)	5, 390
EarI	CTCTTC	2 cut(s)	5, 390
EciI	GGCGGA	1 cut(s)	13
Ecl136II	GAGCTC	1 cut(s)	3332
Eco130I	CCWWGG	1 cut(s)	2956
Eco24I	GRGCYC	4 cut(s)	438, 1911, 2063, 3334
Eco31I	GGTCTC	2 cut(s)	112, 1820
Eco47I	GGWCC	3 cut(s)	1128, 1637, 3020
Eco53kI	GAGCTC	1 cut(s)	3332
Eco57I	CTGAAG	3 cut(s)	1734, 2177, 2576
Eco72I	CACGTG	1 cut(s)	3003
Eco88I	CYCGRG	1 cut(s)	437
EcoICRI	GAGCTC	1 cut(s)	3332
EcoNI	CCTNNNNNAGG	2 cut(s)	168, 3114
EcoO109I	RGGNCCY	1 cut(s)	1908
EcoRII	CCWGG	4 cut(s)	1072, 1436, 1810, 2039
EcoT14I	CCWWGG	1 cut(s)	2956
EcoT22I	ATGCAT	2 cut(s)	2761, 3358
EcoT38I	GRGCYC	4 cut(s)	438, 1911, 2063, 3334
ErhI	CCWWGG	1 cut(s)	2956
Esp3I	CGTCTC	1 cut(s)	3374
FalI	AAGNNNNNCTT	2 cut(s)	632, 664
FauI	CCCGC	3 cut(s)	39, 1303, 3303
FauNDI	CATATG	2 cut(s)	1497, 3048
FbaI	TGATCA	1 cut(s)	565
FblI	GTMKAC	4 cut(s)	223, 2195, 2258, 3139
FokI	GGATG	5 cut(s)	1986, 2023, 2291, 3248, 3388
FriOI	GRGCYC	4 cut(s)	438, 1911, 2063, 3334
FspBI	CTAG	8 cut(s)	170, 380, 845, 950, 1292, 1839, 2330, 2622
FspI	TGCGCA	1 cut(s)	497
GlaI	GCGC	2 cut(s)	497, 789
GsaI	CCCAGC	1 cut(s)	1398
GsuI	CTGGAG	3 cut(s)	908, 2736, 3437
HaeIII	GGCC	6 cut(s)	174, 318, 1077, 1852, 1909, 2692
HapII	CCGG	5 cut(s)	27, 58, 104, 1635, 2262
HgaI	GACGC	3 cut(s)	102, 1177, 2971
HhaI	GCGC	2 cut(s)	498, 790
Hin1I	GRCGYC	2 cut(s)	113, 2982
Hin6I	GCGC	2 cut(s)	496, 788
HinP1I	GCGC	2 cut(s)	496, 788
HincII	GTYRAC	2 cut(s)	224, 2560
HindII	GTYRAC	2 cut(s)	224, 2560
HpaII	CCGG	5 cut(s)	27, 58, 104, 1635, 2262
HphI	GGTGA	8 cut(s)	90, 791, 938, 2611, 2651, 2936, 3161, 3355
Hpy166II	GTNNAC	9 cut(s)	224, 884, 946, 2196, 2259, 2560, 2602, 3028, 3140
Hpy8I	GTNNAC	9 cut(s)	224, 884, 946, 2196, 2259, 2560, 2602, 3028, 3140
Hpy99I	CGWCG	1 cut(s)	1193
HpyCH4III	ACNGT	6 cut(s)	493, 583, 1321, 2207, 2978, 3385
HpyCH4IV	ACGT	4 cut(s)	2192, 3002, 3142, 3240
HpyF3I	CTNAG	8 cut(s)	180, 372, 768, 1560, 1790, 2015, 2291, 3420
HpySE526I	ACGT	4 cut(s)	2192, 3002, 3142, 3240
Hsp92I	GRCGYC	2 cut(s)	113, 2982
HspAI	GCGC	2 cut(s)	496, 788
Ksp22I	TGATCA	1 cut(s)	565
LmnI	GCTCC	6 cut(s)	49, 125, 295, 2684, 3337, 3473
LweI	GCATC	7 cut(s)	907, 1522, 1960, 2001, 2269, 2596, 2830
MaeI	CTAG	8 cut(s)	170, 380, 845, 950, 1292, 1839, 2330, 2622
MaeII	ACGT	4 cut(s)	2192, 3002, 3142, 3240
MaeIII	GTNAC	8 cut(s)	114, 124, 577, 728, 2415, 2719, 3097, 3343
MflI	RGATCY	3 cut(s)	1648, 2737, 3257
MhlI	GDGCHC	6 cut(s)	438, 864, 1911, 2063, 2231, 3334
MlsI	TGGCCA	2 cut(s)	318, 1077
MluNI	TGGCCA	2 cut(s)	318, 1077
MlyI	GAGTC	7 cut(s)	80, 223, 407, 1495, 2155, 2804, 3158
MmeI	TCCRAC	4 cut(s)	892, 1579, 2027, 2246
Mox20I	TGGCCA	2 cut(s)	318, 1077
Mph1103I	ATGCAT	2 cut(s)	2761, 3358
MroXI	GAANNNNTTC	2 cut(s)	644, 1995
MscI	TGGCCA	2 cut(s)	318, 1077
MslI	CAYNNNNRTG	5 cut(s)	335, 986, 1365, 1412, 2702
Msp20I	TGGCCA	2 cut(s)	318, 1077
MspA1I	CMGCKG	2 cut(s)	1745, 3419
MspCI	CTTAAG	2 cut(s)	34, 355
MspI	CCGG	5 cut(s)	27, 58, 104, 1635, 2262
MspR9I	CCNGG	5 cut(s)	1074, 1438, 1812, 2041, 2263
Mva1269I	GAATGC	1 cut(s)	2733
MvaI	CCWGG	4 cut(s)	1074, 1438, 1812, 2041
NciI	CCSGG	1 cut(s)	2263
NcoI	CCATGG	1 cut(s)	2956
NdeI	CATATG	2 cut(s)	1497, 3048
NlaIV	GGNNCC	6 cut(s)	62, 291, 1909, 2515, 2618, 3259
NmeAIII	GCCGAG	1 cut(s)	89
NmuCI	GTSAC	5 cut(s)	114, 124, 2415, 3097, 3343
NsbI	TGCGCA	1 cut(s)	497
NsiI	ATGCAT	2 cut(s)	2761, 3358
NspI	RCATGY	4 cut(s)	723, 2759, 3219, 3360
NspV	TTCGAA	1 cut(s)	1567
OliI	CACNNNNGTG	1 cut(s)	1412
PaeR7I	CTCGAG	1 cut(s)	437
PagI	TCATGA	2 cut(s)	364, 1987
PciI	ACATGT	1 cut(s)	3215
PcsI	WCGNNNNNNNCGW	1 cut(s)	229
PctI	GAATGC	1 cut(s)	2733
PdmI	GAANNNNTTC	2 cut(s)	644, 1995
PfeI	GAWTC	8 cut(s)	1043, 1433, 1564, 1756, 1870, 2287, 2345, 2435
PflMI	CCANNNNNTGG	1 cut(s)	2714
PleI	GAGTC	7 cut(s)	79, 223, 406, 1495, 2155, 2803, 3158
PmaCI	CACGTG	1 cut(s)	3003
PmlI	CACGTG	1 cut(s)	3003
PpsI	GAGTC	7 cut(s)	79, 223, 406, 1495, 2155, 2803, 3158
Ppu21I	YACGTR	1 cut(s)	3003
PscI	ACATGT	1 cut(s)	3215
PshAI	GACNNNNGTC	1 cut(s)	819
PshBI	ATTAAT	1 cut(s)	1275
PsiI	TTATAA	1 cut(s)	800
Psp124BI	GAGCTC	1 cut(s)	3334
Psp6I	CCWGG	4 cut(s)	1072, 1436, 1810, 2039
PspCI	CACGTG	1 cut(s)	3003
PspFI	CCCAGC	1 cut(s)	1394
PspGI	CCWGG	4 cut(s)	1072, 1436, 1810, 2039
PspN4I	GGNNCC	6 cut(s)	62, 291, 1909, 2515, 2618, 3259
PspOMI	GGGCCC	1 cut(s)	1907
PspPI	GGNCC	5 cut(s)	1128, 1637, 1907, 1908, 3020
PstNI	CAGNNNCTG	1 cut(s)	2549
PsuI	RGATCY	3 cut(s)	1648, 2737, 3257
PvuII	CAGCTG	2 cut(s)	1745, 3419
RsaI	GTAC	6 cut(s)	490, 1526, 2075, 2267, 2404, 2975
RsaNI	GTAC	6 cut(s)	489, 1525, 2074, 2266, 2403, 2974
RseI	CAYNNNNRTG	5 cut(s)	335, 986, 1365, 1412, 2702
SacI	GAGCTC	1 cut(s)	3334
SalI	GTCGAC	1 cut(s)	222
Sau96I	GGNCC	5 cut(s)	1128, 1637, 1907, 1908, 3020
SchI	GAGTC	7 cut(s)	80, 223, 407, 1495, 2155, 2804, 3158
ScrFI	CCNGG	5 cut(s)	1074, 1438, 1812, 2041, 2263
SduI	GDGCHC	6 cut(s)	438, 864, 1911, 2063, 2231, 3334
SfaNI	GCATC	7 cut(s)	907, 1522, 1960, 2001, 2269, 2596, 2830
SfcI	CTRYAG	1 cut(s)	2304
Sfr274I	CTCGAG	1 cut(s)	437
SfuI	TTCGAA	1 cut(s)	1567
SinI	GGWCC	3 cut(s)	1128, 1637, 3020
SlaI	CTCGAG	1 cut(s)	437
SmiMI	CAYNNNNRTG	5 cut(s)	335, 986, 1365, 1412, 2702
SmlI	CTYRAG	5 cut(s)	34, 355, 437, 926, 1097
SmoI	CTYRAG	5 cut(s)	34, 355, 437, 926, 1097
SpeI	ACTAGT	1 cut(s)	2329
SspI	AATATT	3 cut(s)	1546, 1864, 1894
SspMI	CTAG	8 cut(s)	170, 380, 845, 950, 1292, 1839, 2330, 2622
SstI	GAGCTC	1 cut(s)	3334
StyD4I	CCNGG	5 cut(s)	1072, 1436, 1810, 2039, 2261
StyI	CCWWGG	1 cut(s)	2956
TaaI	ACNGT	6 cut(s)	493, 583, 1321, 2207, 2978, 3385
TaiI	ACGT	4 cut(s)	2195, 3005, 3145, 3243
TatI	WGTACW	1 cut(s)	2973
TfiI	GAWTC	8 cut(s)	1043, 1433, 1564, 1756, 1870, 2287, 2345, 2435
TscAI	CASTG	6 cut(s)	1620, 1758, 2023, 2084, 2413, 2983
TseFI	GTSAC	5 cut(s)	114, 124, 2415, 3097, 3343
Tsp45I	GTSAC	5 cut(s)	114, 124, 2415, 3097, 3343
TspGWI	ACGGA	3 cut(s)	112, 621, 2357
TspRI	CASTG	6 cut(s)	1620, 1758, 2023, 2084, 2413, 2983
Van91I	CCANNNNNTGG	1 cut(s)	2714
Vha464I	CTTAAG	2 cut(s)	34, 355
VpaK11BI	GGWCC	3 cut(s)	1128, 1637, 3020
VspI	ATTAAT	1 cut(s)	1275
XagI	CCTNNNNNAGG	2 cut(s)	168, 3114
XapI	RAATTY	9 cut(s)	234, 310, 702, 737, 968, 1252, 1995, 2645, 3062
XbaI	TCTAGA	3 cut(s)	844, 1291, 1838
XceI	RCATGY	4 cut(s)	723, 2759, 3219, 3360
XcmI	CCANNNNNNNNNTGG	1 cut(s)	772
XhoI	CTCGAG	1 cut(s)	437
XmiI	GTMKAC	4 cut(s)	223, 2195, 2258, 3139
XmnI	GAANNNNTTC	2 cut(s)	644, 1995
XspI	CTAG	8 cut(s)	170, 380, 845, 950, 1292, 1839, 2330, 2622
Zsp2I	ATGCAT	2 cut(s)	2761, 3358

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.