Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0005765

Physical Location & Seq

Genomic Coordinates Reverse (-)

30450692 .. 30463615

Gene Structure 12924 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1237100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3507 bp

ATGACAACGTCGGAGAGAGAAGAGAAGCCGACGAAGATGTTCGGGAAGCAATTGGCGGCGGCGGAGGACTTCGCGGCGGTGCGTGAGCTCTTCCGCCACCACATAGAGTCATTCGACTACCTTGTCACTAAGGGTCTCGAGGTTCTACTCAGCAAGATCAAGCCCATCGAAATCGTCGACCCTTCCACCAACAATACGCTTACAAATATCCTTTCTTTTATTTTCCTTGCATTTTTATCTGCAATTTGTTTTTGTAGACAGGCTAAACTTTCCTACACGGGGAAATTCATGGCGGAAGTTTGCTTTAAGTATAATGATAGTGGAGCTGTTATAAGAGAAAAGTTCAATTTTGGGCAGCTTCCCATCATGCTACAGTCAAAGCTTTGCCACTTAAGAGGTGCTACTCCCCAAAAGCTAGTTTCTTACAAAGAAGAGGCATCGGAAATGGGTGGTTACTTCATTTTAAATGGGCTTGAGAGAGTGGTTCGGCTTGTTATATTGCCAAAGCGGAACTATCCAACTAGTATGGTGCGAAATTCATTTCGTGATCGAAAGGAAGGATATACTGACAAAGCAGTTGTAATAAGATGTGAAAGAGAAGATCAGTCTTCAGTAACTGTCAAATTGTATTACCTTCGTAATGGAAGTGCAAGACTGGGATTTTGGATACAAGGGAGGGAGTACTTGCTTCCTGTAGGCGTTGTCTTAAAGGCTCTCATTGATACTACTGATCGTGAAATTTATGAGTGTTTGACATGTTGCTATGTCGAGAACTATGAGAAAGGAAAAGGGGCTGTTGGCACTCAGCTAGTGGGTGAGAGGGCACAAATTATCCTGGATGAAGTGAGAGACTTGAAACTTTTCACTCGTCATGAATGTCTAGAGCACATTGGAGAACACTTCCGACCTCTTATGGGTGGCTTGGAGAATGAACGACCTTCGGTTGTTGGGGATGCTGTTATAATGAATTACATATTGGTCCACCTTAATAAGGCTCATGACAAATTCAATCTGCTCATATTTATGGTGCAGAAACTTTTTTCACTTGTGGATCAGACTTCTGTGCTGGACAACCCAGATTCCTTGCAAAATCATGAAGTCCTACTCCCTGGTCACTTGATTACAATATATCTTAAGGAAAAACTAGAAGAGTGGTTGCAAAGGACGAAAAAAATTATTGAAGATGAGATCAACAAAAATAAGGACTTTGATTTTGGCAACGTAACTAAAGTAAAGAAGCTTATGGATAAAAATCCTGCAAAGCAGATCAGTTTAGCTGTTGAGAATATGTTGAAAACTGGAAGACTTAATACTCAATCTGGTCTAGATTTGCAGCAGAGGGCAGGCTTGACCGTTCAGGCAGAGAGGCTTAACTATTTACGTTTCATATCTCATTTTCGTGCTGTTCATCGAGGGGCATCATTCGCTGGACTTCGCACCACAAGTGTGCGAAAATTGTTACCTGAATCTTGGGGTTTTCTTTGCCCTGTCCATACACCTGATGGTGAACCATGTGGCTTGTTGAATCATATGACCCACATTTGTAGAATTACATCCTTCTTTGATGCTCAAGGAAATATTAGAGACTTCTCAAAGATCCGAGAGTCTATTCTCAGTGTTCTGAGTGCAGTTGGAATGAAGACTTCAAAGCCAAATCTGGTTCAAGCTGGTCCTCCTGCAGCTTTTTCTGTTTTGTTAGATGGCTGTGTTGTTGGTTCTATATCTTCCAGTAAAGTTGAGAAAGTTGTGGCTCATTTAAGGAGGCTGAAAGTGTCTGCTGCTCCAGTGATTCCTGAGGATCTGGAAGTGGGATATGTTCCGTTAAGCATGGGTGGAGCATATCCTGGTCTGTACCTTTTCACTATCCCTTCTAGATTTGTTCGGCCAGTAAAGAATATTTCAATTCCTTCCGTTGAAGGTCAGAATATTGAACTTATTGGACCCTTTGAACAGGTTTTCATGGAAATAAGATGTCCTGATGGTCGAGATGGTGGAAGGAAAGATGGTTTCCCAGCAACTCATGAAGAAATTCATCCAACTGGAATGCTCAGTGTGGTCGCTAATCTTACACCCTGGTCAGATCATAATCAGAGCCCACGAAATATGTATCAGTGCCAGATGGCAAAACAAACAATGGCCTTCTCTTTACAAGGACTTTGCAATCGTGCAGATCAAAAGTTGTACCATCTTCAGACTCCTCAGTCCCCTATTGTACGCACAAGTGCATATAAGAAGTACTGCATCGATGAATACCCAACAGGCACAAATGCGATCGTTGCTGTACTGTCATATACTGGATATGATATGGAGGATGCCATGATTCTAAATAAGTCATCTGTCGAACGCGGAATGTTTCATGGGCATATATATCAGACAGAAACAATAGACTTGTCTGATCAAAAGAAGAGGTCGGATAACACCCCAACATATTTTAAGAAAAGTAACGTCGATAAAAATCCTTTGATTGATTCAGATGGTCTTCCTCATGTTGGTCAGACAATAAAACCATATGAAGAATATTATAGCTTTACAAATGACACTCTTCATAAGCCCAGAGCCGTCAAACGGAAAGGTACAGAACCTGTTATAGTTGACTACGTTGCTATTGATGGGAAGAAACTCCTCCAGAAGGCAAACATACGCTTTCGACACACTAGAAACCCCATCATTGGTGATAAATTTAGCAGCCGGCATGGGCAAAAAGGTGTTTGCTCCCAGTTGTGGCCTGATATTGATATGCCATTTTCTGGGGTTACGGGAATGCGTCCAGATCTAATCATCAATCCCCATGCATTTCCTTCTAGAATGACAATAGCTATGCTGTTGGAATCAGTTGCTGCAAAGGGCGGTAGCTTACACGGAAACTATGTTGACGCGACTCCATTTGCTAGTTCAAAGAATGCTAATGGAGAGACTGGACCAAAATCCAAAACCCTTGTTGACGAACTTGGTGAAATGTTAAAGGTTAAAGGGTTTAACTACCATGGTGTAGAAGTTCTGTACAGCGGGGTTTATGGCACAGAACTCACGTGCGAGATCTTTATTGGCCCTGTTTATTATCAACGACTACGACATATGGTTTCAGACAAATTTCAGGTTCGCTCCACTGGAACGGTAGACCAGGTCACTCGCCAGCCTATCAAAGGACGAAAGCGAGGTGGTGGCATACGTTTTGGTGAAATGGAGCGCGACTCCATGCTTGCGCATGGGGCAGCATATCTGTTGCACGACAGGCTTCATACCTGTTCTGATTATCACATTGCCGATGTATGTTCCATATGTGGAAGCATCCTGACGTCCACATTCATCCAACCAAAACGGGTTGGGCGTGCGGTTTCAGGAATTCCGTCTGTAAGAGCTCCCAAGAAGGTCATCTGCCATGCTTGTAAGACAAGTAAAGGGATGGAGACAGTTGCAATGCCCTATGTCTTCAGATATTTAGCTGCAGAGTTGGCGTCAATGAACATAAAAATGACTCTTCAGCTAAGTAATGCAGCGAAAGATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000428 GO:0003674 GO:0003824 GO:0003899 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005736 GO:0005737 GO:0005829 GO:0006139 GO:0006351 GO:0006360 GO:0006725 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009553 GO:0009561 GO:0009987 GO:0010467 GO:0016070 GO:0016740 GO:0016772 GO:0016779 GO:0018130 GO:0019438 GO:0030880 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032774 GO:0032991 GO:0034062 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044452 GO:0044464 GO:0046483 GO:0048229 GO:0048856 GO:0055029 GO:0061695 GO:0070013 GO:0071704 GO:0090304 GO:0097659 GO:0097747 GO:0140098 GO:1901360 GO:1901362 GO:1901576 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko00230 ko00240 ko01100 ko03020 map00230 map00240 map01100 map03020

Pfam Domains

Protein Families

RNA_pol_Rpa2_4 RNA_pol_Rpb2_1 RNA_pol_Rpb2_2 RNA_pol_Rpb2_3 RNA_pol_Rpb2_6 RNA_pol_Rpb2_7

Protein Analysis

1169

Amino Acids

131.05

Weight (kDa)

9.02

Isoelectric Point (pI)

35.21

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RNA_pol_Rpb2_1	PF04563	85 - 413	8.9e-25	RNA polymerase beta subunit
RNA_pol_Rpb2_2	PF04561	195 - 367	2e-10	RNA polymerase Rpb2, domain 2
RNA_pol_Rpb2_3	PF04565	454 - 517	6.8e-28	RNA polymerase Rpb2, domain 3
RNA_pol_Rpa2_4	PF06883	571 - 628	3.7e-20	RNA polymerase I, Rpa2 specific domain
RNA_pol_Rpb2_6	PF00562	686 - 1052	2.3e-117	RNA polymerase Rpb2, domain 6
RNA_pol_Rpb2_7	PF04560	1054 - 1161	1e-21	RNA polymerase Rpb2, domain 7

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0005765)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G29940
fragaria_vesca	FvH4_5g25870 FvH4_5g25870 FvH4_7g07420 FvH4_7g07420 FvH4_7g07420 FvH4_7g07420 FvH4_7g07420 FvH4_7g07420 FvH4_7g07420
malus_domestica	MD08G1237100.v1.1 MD15G1428900.v1.1
prunus_persica	Prupe.1G574600_v2.0.a1 Prupe.1G574600_v2.0.a1 Prupe.7G107000_v2.0.a1 Prupe.7G184700_v2.0.a1 Prupe.7G223000_v2.0.a1
pyrus_communis	pycom08g20550
rosa_chinensis	RchiOBHm_Chr1g0339571 RchiOBHm_Chr1g0339591
rosa_laevigata	RLG00000029239
rosa_multiflora	Rmu_sc0015580.1_g000004
rosa_roxburghii	Rroxscaffold_4G00313560 Rroxscaffold_4G00313600
rosa_rugosa	Rorug01G0140100.1
rosa_samantha	Rh1AG158100 Rh1CG147600
rosa_wichuraiana	Rw1G013100

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	332, 962
AasI	GACNNNNNNGTC	2 cut(s)	122, 2200
AatII	GACGTC	1 cut(s)	3299
Acc16I	TGCGCA	1 cut(s)	3204
AccB7I	CCANNNNNTGG	1 cut(s)	2747
AccI	GTMKAC	3 cut(s)	177, 256, 3117
AccII	CGCG	4 cut(s)	74, 2346, 2876, 3189
AclWI	GGATC	3 cut(s)	1059, 1591, 1806
AcoI	YGGCCR	1 cut(s)	1883
AcsI	RAATTY	8 cut(s)	284, 535, 738, 1004, 2028, 2678, 3089, 3342
AcuI	CTGAAG	4 cut(s)	594, 2174, 3415, 3464
AcvI	CACGTG	1 cut(s)	3030
AcyI	GRCGYC	2 cut(s)	3296, 3455
AfaI	GTAC	8 cut(s)	683, 1853, 2183, 2214, 2237, 2283, 2575, 3002
AflII	CTTAAG	2 cut(s)	391, 1133
AflIII	ACRYGT	1 cut(s)	755
AhlI	ACTAGT	1 cut(s)	521
AjnI	CCWGG	5 cut(s)	834, 1108, 1843, 2072, 3120
AjuI	GAANNNNNNNTTGG	2 cut(s)	959, 991
AleI	CACNNNNGTG	1 cut(s)	1445
Alw21I	GWGCWC	3 cut(s)	90, 888, 3361
Alw26I	GTCTC	5 cut(s)	140, 843, 1578, 2906, 3401
AlwI	GGATC	3 cut(s)	1059, 1591, 1806
AlwNI	CAGNNNCTG	3 cut(s)	617, 2582, 2837
Ama87I	CYCGRG	1 cut(s)	137
AoxI	GGCC	4 cut(s)	1883, 2136, 2723, 3046
ApeKI	GCWGC	9 cut(s)	355, 1333, 1679, 1778, 2685, 2837, 3212, 3443, 3494
ApoI	RAATTY	8 cut(s)	284, 535, 738, 1004, 2028, 2678, 3089, 3342
ArsI	GACNNNNNNTTYG	4 cut(s)	645, 677, 1196, 1228
Asp700I	GAANNNNTTC	3 cut(s)	38, 860, 2028
AspLEI	GCGC	2 cut(s)	3189, 3205
AspS9I	GGNCC	5 cut(s)	979, 1670, 1940, 2918, 3047
AsuHPI	GGTGA	5 cut(s)	827, 1517, 2684, 2963, 3188
AvaI	CYCGRG	1 cut(s)	137
AvaII	GGWCC	4 cut(s)	979, 1670, 1940, 2918
AxyI	CCTNAGG	1 cut(s)	1794
BaeGI	GKGCMC	1 cut(s)	826
BanII	GRGCYC	3 cut(s)	90, 2096, 3361
BarI	GAAGNNNNNNTAC	2 cut(s)	1294, 1326
BbrPI	CACGTG	1 cut(s)	3030
BbsI	GAAGAC	5 cut(s)	600, 1309, 1646, 2471, 3421
Bbv12I	GWGCWC	3 cut(s)	90, 888, 3361
BbvI	GCAGC	8 cut(s)	367, 1345, 1691, 1765, 2697, 2824, 3224, 3430
BceAI	ACGGC	1 cut(s)	2543
BciT130I	CCWGG	5 cut(s)	836, 1110, 1845, 2074, 3122
BciVI	GTATCC	1 cut(s)	660
BclI	TGATCA	1 cut(s)	2395
BcoDI	GTCTC	5 cut(s)	140, 843, 1578, 2906, 3401
BcuI	ACTAGT	1 cut(s)	521
BfmI	CTRYAG	4 cut(s)	371, 693, 1677, 3444
BfrI	CTTAAG	2 cut(s)	391, 1133
BfuI	GTATCC	1 cut(s)	660
BglII	AGATCT	2 cut(s)	2770, 3036
BmcAI	AGTACT	2 cut(s)	683, 2237
Bme1390I	CCNGG	5 cut(s)	836, 1110, 1845, 2074, 3122
Bme18I	GGWCC	4 cut(s)	979, 1670, 1940, 2918
BmeT110I	CYCGRG	1 cut(s)	137
BmgT120I	GGNCC	5 cut(s)	979, 1670, 1940, 2918, 3047
BmiI	GGNNCC	1 cut(s)	1942
BmrFI	CCNGG	5 cut(s)	836, 1110, 1845, 2074, 3122
BmrI	ACTGGG	2 cut(s)	665, 2710
BmsI	GCATC	7 cut(s)	446, 943, 1427, 1555, 2250, 2302, 3297
BmuI	ACTGGG	2 cut(s)	665, 2710
BpiI	GAAGAC	5 cut(s)	600, 1309, 1646, 2471, 3421
BplI	GAGNNNNNCTC	2 cut(s)	3176, 3208
BpmI	CTGGAG	2 cut(s)	1767, 2609
BpuEI	CTTGAG	2 cut(s)	494, 1554
Bsa29I	ATCGAT	1 cut(s)	2244
BsaAI	YACGTR	1 cut(s)	3030
BsaBI	GATNNNNATC	3 cut(s)	1251, 2085, 2454
BsaHI	GRCGYC	2 cut(s)	3296, 3455
BsaI	GGTCTC	1 cut(s)	140
BsaJI	CCNNGG	3 cut(s)	1108, 2072, 2983
Bse118I	RCCGGY	1 cut(s)	2688
Bse21I	CCTNAGG	1 cut(s)	1794
Bse3DI	GCAATG	2 cut(s)	3258, 3423
Bse8I	GATNNNNATC	3 cut(s)	1251, 2085, 2454
BseBI	CCWGG	5 cut(s)	836, 1110, 1845, 2074, 3122
BseCI	ATCGAT	1 cut(s)	2244
BseDI	CCNNGG	3 cut(s)	1108, 2072, 2983
BseGI	GGATG	8 cut(s)	844, 958, 1553, 2032, 2317, 3288, 3306, 3408
BseJI	GATNNNNATC	3 cut(s)	1251, 2085, 2454
BseMI	GCAATG	2 cut(s)	3258, 3423
BseMII	CTCAG	7 cut(s)	163, 818, 1613, 1627, 1785, 2062, 2213
BseRI	GAGGAG	2 cut(s)	2187, 2612
BseSI	GKGCMC	1 cut(s)	826
BseXI	GCAGC	8 cut(s)	367, 1345, 1691, 1765, 2697, 2824, 3224, 3430
BseYI	CCCAGC	1 cut(s)	2011
BsgI	GTGCAG	3 cut(s)	1049, 1647, 2187
Bsh1236I	CGCG	4 cut(s)	74, 2346, 2876, 3189
Bsh1285I	CGRYCG	1 cut(s)	2274
BshFI	GGCC	4 cut(s)	1885, 2138, 2725, 3048
BshVI	ATCGAT	1 cut(s)	2244
BsiEI	CGRYCG	1 cut(s)	2274
BsiHKAI	GWGCWC	3 cut(s)	90, 888, 3361
BsiHKCI	CYCGRG	1 cut(s)	137
BsiSI	CCGG	1 cut(s)	2689
BslFI	GGGAC	1 cut(s)	2188
BsmAI	GTCTC	5 cut(s)	140, 843, 1578, 2906, 3401
BsmFI	GGGAC	1 cut(s)	2188
BsmI	GAATGC	3 cut(s)	2049, 2766, 2905
BsnI	GGCC	4 cut(s)	1885, 2138, 2725, 3048
Bso31I	GGTCTC	1 cut(s)	140
BsoBI	CYCGRG	1 cut(s)	137
Bsp1286I	GDGCHC	5 cut(s)	90, 826, 888, 2096, 3361
Bsp1407I	TGTACA	1 cut(s)	3000
Bsp19I	CCATGG	1 cut(s)	2983
BspANI	GGCC	4 cut(s)	1885, 2138, 2725, 3048
BspCNI	CTCAG	7 cut(s)	162, 817, 1614, 1626, 1786, 2061, 2212
BspDI	ATCGAT	1 cut(s)	2244
BspFNI	CGCG	4 cut(s)	74, 2346, 2876, 3189
BspHI	TCATGA	4 cut(s)	871, 997, 1093, 2020
BspLI	GGNNCC	1 cut(s)	1942
BspMAI	CTGCAG	2 cut(s)	1681, 3448
BspPI	GGATC	3 cut(s)	1059, 1591, 1806
BspQI	GCTCTTC	1 cut(s)	95
BspTI	CTTAAG	2 cut(s)	391, 1133
BspTNI	GGTCTC	1 cut(s)	140
BsrDI	GCAATG	2 cut(s)	3258, 3423
BsrFI	RCCGGY	1 cut(s)	2688
BsrGI	TGTACA	1 cut(s)	3000
BssAI	RCCGGY	1 cut(s)	2688
BssECI	CCNNGG	3 cut(s)	1108, 2072, 2983
BssNI	GRCGYC	2 cut(s)	3296, 3455
BssT1I	CCWWGG	1 cut(s)	2983
Bst2UI	CCWGG	5 cut(s)	836, 1110, 1845, 2074, 3122
Bst4CI	ACNGT	6 cut(s)	375, 619, 1354, 2286, 3115, 3412
Bst6I	CTCTTC	7 cut(s)	15, 95, 426, 1143, 2399, 2547, 3483
BstACI	GRCGYC	2 cut(s)	3296, 3455
BstAFI	CTTAAG	2 cut(s)	391, 1133
BstAUI	TGTACA	1 cut(s)	3000
BstBAI	YACGTR	1 cut(s)	3030
BstC8I	GCNNGC	5 cut(s)	1345, 2690, 3134, 3201, 3330
BstDEI	CTNAG	9 cut(s)	129, 149, 804, 1613, 1622, 1794, 2048, 2199, 3485
BstDSI	CCRYGG	1 cut(s)	2983
BstENI	CCTNNNNNAGG	3 cut(s)	989, 2627, 3141
BstF5I	GGATG	8 cut(s)	844, 958, 1553, 2032, 2317, 3288, 3306, 3408
BstFNI	CGCG	4 cut(s)	74, 2346, 2876, 3189
BstHHI	GCGC	2 cut(s)	3189, 3205
BstMAI	GTCTC	5 cut(s)	140, 843, 1578, 2906, 3401
BstMCI	CGRYCG	1 cut(s)	2274
BstMWI	GCNNNNNNNGC	5 cut(s)	1424, 2276, 3209, 3232, 3452
BstNI	CCWGG	5 cut(s)	836, 1110, 1845, 2074, 3122
BstNSI	RCATGY	1 cut(s)	759
BstSCI	CCNGG	5 cut(s)	834, 1108, 1843, 2072, 3120
BstSFI	CTRYAG	4 cut(s)	371, 693, 1677, 3444
BstSLI	GKGCMC	1 cut(s)	826
BstUI	CGCG	4 cut(s)	74, 2346, 2876, 3189
BstV1I	GCAGC	8 cut(s)	367, 1345, 1691, 1765, 2697, 2824, 3224, 3430
BstV2I	GAAGAC	5 cut(s)	600, 1309, 1646, 2471, 3421
BstX2I	RGATCY	4 cut(s)	1596, 1798, 2770, 3036
BstYI	RGATCY	4 cut(s)	1596, 1798, 2770, 3036
Bsu15I	ATCGAT	1 cut(s)	2244
Bsu36I	CCTNAGG	1 cut(s)	1794
BsuI	GTATCC	1 cut(s)	660
BsuRI	GGCC	4 cut(s)	1885, 2138, 2725, 3048
BsuTUI	ATCGAT	1 cut(s)	2244
BtgI	CCRYGG	1 cut(s)	2983
BtsCI	GGATG	8 cut(s)	844, 958, 1553, 2032, 2317, 3288, 3306, 3408
BtsIMutI	CAGTG	5 cut(s)	1621, 1791, 2056, 2117, 3105
Cac8I	GCNNGC	5 cut(s)	1345, 2690, 3134, 3201, 3330
CaiI	CAGNNNCTG	3 cut(s)	617, 2582, 2837
CciI	TCATGA	4 cut(s)	871, 997, 1093, 2020
CfoI	GCGC	2 cut(s)	3189, 3205
Cfr10I	RCCGGY	1 cut(s)	2688
Cfr13I	GGNCC	5 cut(s)	979, 1670, 1940, 2918, 3047
ClaI	ATCGAT	1 cut(s)	2244
CseI	GACGC	3 cut(s)	2753, 2882, 3444
CsiI	ACCWGGT	1 cut(s)	3120
Csp6I	GTAC	8 cut(s)	682, 1852, 2182, 2213, 2236, 2282, 2574, 3001
CviQI	GTAC	8 cut(s)	682, 1852, 2182, 2213, 2236, 2282, 2574, 3001
DdeI	CTNAG	9 cut(s)	129, 149, 804, 1613, 1622, 1794, 2048, 2199, 3485
DraI	TTTAAA	1 cut(s)	465
DrdI	GACNNNNNNGTC	2 cut(s)	122, 2200
DseDI	GACNNNNNNGTC	2 cut(s)	122, 2200
EaeI	YGGCCR	1 cut(s)	1883
Eam1104I	CTCTTC	7 cut(s)	15, 95, 426, 1143, 2399, 2547, 3483
EarI	CTCTTC	7 cut(s)	15, 95, 426, 1143, 2399, 2547, 3483
EciI	GGCGGA	3 cut(s)	77, 83, 308
Ecl136II	GAGCTC	2 cut(s)	88, 3359
Eco130I	CCWWGG	1 cut(s)	2983
Eco24I	GRGCYC	3 cut(s)	90, 2096, 3361
Eco31I	GGTCTC	1 cut(s)	140
Eco47I	GGWCC	4 cut(s)	979, 1670, 1940, 2918
Eco53kI	GAGCTC	2 cut(s)	88, 3359
Eco57I	CTGAAG	4 cut(s)	594, 2174, 3415, 3464
Eco72I	CACGTG	1 cut(s)	3030
Eco81I	CCTNAGG	1 cut(s)	1794
Eco88I	CYCGRG	1 cut(s)	137
EcoICRI	GAGCTC	2 cut(s)	88, 3359
EcoNI	CCTNNNNNAGG	3 cut(s)	989, 2627, 3141
EcoRI	GAATTC	1 cut(s)	3342
EcoRII	CCWGG	5 cut(s)	834, 1108, 1843, 2072, 3120
EcoT14I	CCWWGG	1 cut(s)	2983
EcoT22I	ATGCAT	1 cut(s)	2794
EcoT38I	GRGCYC	3 cut(s)	90, 2096, 3361
ErhI	CCWWGG	1 cut(s)	2983
FaqI	GGGAC	1 cut(s)	2188
FauI	CCCGC	1 cut(s)	2999
FauNDI	CATATG	4 cut(s)	1530, 2509, 3075, 3278
FbaI	TGATCA	1 cut(s)	2395
FblI	GTMKAC	3 cut(s)	177, 256, 3117
FokI	GGATG	8 cut(s)	851, 965, 1540, 2019, 2324, 3275, 3293, 3415
FriOI	GRGCYC	3 cut(s)	90, 2096, 3361
FspI	TGCGCA	1 cut(s)	3204
GlaI	GCGC	2 cut(s)	3188, 3204
GsaI	CCCAGC	1 cut(s)	2015
GsuI	CTGGAG	2 cut(s)	1767, 2609
HaeIII	GGCC	4 cut(s)	1885, 2138, 2725, 3048
HapII	CCGG	1 cut(s)	2689
HgaI	GACGC	3 cut(s)	2753, 2882, 3444
HhaI	GCGC	2 cut(s)	3189, 3205
Hin1I	GRCGYC	2 cut(s)	3296, 3455
Hin6I	GCGC	2 cut(s)	3187, 3203
HinP1I	GCGC	2 cut(s)	3187, 3203
HincII	GTYRAC	4 cut(s)	178, 2593, 2872, 2941
HindII	GTYRAC	4 cut(s)	178, 2593, 2872, 2941
HindIII	AAGCTT	2 cut(s)	380, 1238
HpaII	CCGG	1 cut(s)	2689
HphI	GGTGA	5 cut(s)	827, 1517, 2684, 2963, 3188
Hpy166II	GTNNAC	9 cut(s)	178, 257, 982, 1508, 2593, 2872, 2941, 3118, 3300
Hpy8I	GTNNAC	9 cut(s)	178, 257, 982, 1508, 2593, 2872, 2941, 3118, 3300
Hpy99I	CGWCG	4 cut(s)	13, 34, 179, 2450
HpyCH4III	ACNGT	6 cut(s)	375, 619, 1354, 2286, 3115, 3412
HpyCH4IV	ACGT	8 cut(s)	8, 1221, 1381, 2445, 2598, 3029, 3169, 3296
HpyF10VI	GCNNNNNNNGC	5 cut(s)	1424, 2276, 3209, 3232, 3452
HpyF3I	CTNAG	9 cut(s)	129, 149, 804, 1613, 1622, 1794, 2048, 2199, 3485
HpySE526I	ACGT	8 cut(s)	8, 1221, 1381, 2445, 2598, 3029, 3169, 3296
Hsp92I	GRCGYC	2 cut(s)	3296, 3455
HspAI	GCGC	2 cut(s)	3187, 3203
KroI	GCCGGC	1 cut(s)	2688
KroNI	GCCGGC	1 cut(s)	2690
Ksp22I	TGATCA	1 cut(s)	2395
LguI	GCTCTTC	1 cut(s)	95
LmnI	GCTCC	7 cut(s)	323, 1786, 1835, 2717, 3107, 3184, 3364
Lsp1109I	GCAGC	8 cut(s)	367, 1345, 1691, 1765, 2697, 2824, 3224, 3430
LweI	GCATC	7 cut(s)	446, 943, 1427, 1555, 2250, 2302, 3297
MabI	ACCWGGT	1 cut(s)	3120
MaeII	ACGT	8 cut(s)	8, 1221, 1381, 2445, 2598, 3029, 3169, 3296
MaeIII	GTNAC	9 cut(s)	124, 452, 613, 1112, 1222, 1458, 2441, 2752, 3124
MfeI	CAATTG	1 cut(s)	50
MflI	RGATCY	4 cut(s)	1596, 1798, 2770, 3036
MhlI	GDGCHC	5 cut(s)	90, 826, 888, 2096, 3361
MlyI	GAGTC	6 cut(s)	116, 1613, 2188, 2872, 3185, 3469
MmeI	TCCRAC	7 cut(s)	542, 928, 1612, 2060, 2391, 2805, 3334
Mph1103I	ATGCAT	1 cut(s)	2794
MroNI	GCCGGC	1 cut(s)	2688
MroXI	GAANNNNTTC	3 cut(s)	38, 860, 2028
MslI	CAYNNNNRTG	4 cut(s)	1022, 1398, 1445, 3470
MspA1I	CMGCKG	1 cut(s)	3006
MspCI	CTTAAG	2 cut(s)	391, 1133
MspI	CCGG	1 cut(s)	2689
MspR9I	CCNGG	5 cut(s)	836, 1110, 1845, 2074, 3122
MunI	CAATTG	1 cut(s)	50
Mva1269I	GAATGC	3 cut(s)	2049, 2766, 2905
MvaI	CCWGG	5 cut(s)	836, 1110, 1845, 2074, 3122
MvnI	CGCG	4 cut(s)	74, 2346, 2876, 3189
MwoI	GCNNNNNNNGC	5 cut(s)	1424, 2276, 3209, 3232, 3452
NaeI	GCCGGC	1 cut(s)	2690
NcoI	CCATGG	1 cut(s)	2983
NdeI	CATATG	4 cut(s)	1530, 2509, 3075, 3278
NgoMIV	GCCGGC	1 cut(s)	2688
NlaIV	GGNNCC	1 cut(s)	1942
NmuCI	GTSAC	3 cut(s)	124, 1112, 3124
NsbI	TGCGCA	1 cut(s)	3204
NsiI	ATGCAT	1 cut(s)	2794
NspI	RCATGY	1 cut(s)	759
OliI	CACNNNNGTG	1 cut(s)	1445
PaeR7I	CTCGAG	1 cut(s)	137
PagI	TCATGA	4 cut(s)	871, 997, 1093, 2020
PciI	ACATGT	1 cut(s)	755
PciSI	GCTCTTC	1 cut(s)	95
PcsI	WCGNNNNNNNCGW	2 cut(s)	174, 3325
PctI	GAATGC	3 cut(s)	2049, 2766, 2905
PdiI	GCCGGC	1 cut(s)	2690
PdmI	GAANNNNTTC	3 cut(s)	38, 860, 2028
PfeI	GAWTC	7 cut(s)	1079, 1466, 1525, 1789, 2320, 2468, 2828
PflFI	GACNNNGTC	2 cut(s)	7, 3122
PflMI	CCANNNNNTGG	1 cut(s)	2747
PfoI	TCCNGGA	1 cut(s)	834
Ple19I	CGATCG	1 cut(s)	2274
PleI	GAGTC	6 cut(s)	115, 1612, 2188, 2872, 3185, 3469
PmaCI	CACGTG	1 cut(s)	3030
PmlI	CACGTG	1 cut(s)	3030
PpsI	GAGTC	6 cut(s)	115, 1612, 2188, 2872, 3185, 3469
Ppu21I	YACGTR	1 cut(s)	3030
PscI	ACATGT	1 cut(s)	755
PsiI	TTATAA	2 cut(s)	332, 962
Psp124BI	GAGCTC	2 cut(s)	90, 3361
Psp6I	CCWGG	5 cut(s)	834, 1108, 1843, 2072, 3120
PspCI	CACGTG	1 cut(s)	3030
PspFI	CCCAGC	1 cut(s)	2011
PspGI	CCWGG	5 cut(s)	834, 1108, 1843, 2072, 3120
PspN4I	GGNNCC	1 cut(s)	1942
PspPI	GGNCC	5 cut(s)	979, 1670, 1940, 2918, 3047
PstI	CTGCAG	2 cut(s)	1681, 3448
PstNI	CAGNNNCTG	3 cut(s)	617, 2582, 2837
PsuI	RGATCY	4 cut(s)	1596, 1798, 2770, 3036
PsyI	GACNNNGTC	2 cut(s)	7, 3122
PvuI	CGATCG	1 cut(s)	2274
RsaI	GTAC	8 cut(s)	683, 1853, 2183, 2214, 2237, 2283, 2575, 3002
RsaNI	GTAC	8 cut(s)	682, 1852, 2182, 2213, 2236, 2282, 2574, 3001
RseI	CAYNNNNRTG	4 cut(s)	1022, 1398, 1445, 3470
SacI	GAGCTC	2 cut(s)	90, 3361
SalI	GTCGAC	1 cut(s)	176
SapI	GCTCTTC	1 cut(s)	95
Sau96I	GGNCC	5 cut(s)	979, 1670, 1940, 2918, 3047
ScaI	AGTACT	2 cut(s)	683, 2237
SchI	GAGTC	6 cut(s)	116, 1613, 2188, 2872, 3185, 3469
ScrFI	CCNGG	5 cut(s)	836, 1110, 1845, 2074, 3122
SduI	GDGCHC	5 cut(s)	90, 826, 888, 2096, 3361
SexAI	ACCWGGT	1 cut(s)	3120
SfaNI	GCATC	7 cut(s)	446, 943, 1427, 1555, 2250, 2302, 3297
SfcI	CTRYAG	4 cut(s)	371, 693, 1677, 3444
Sfr274I	CTCGAG	1 cut(s)	137
SinI	GGWCC	4 cut(s)	979, 1670, 1940, 2918
SlaI	CTCGAG	1 cut(s)	137
SmiMI	CAYNNNNRTG	4 cut(s)	1022, 1398, 1445, 3470
SmlI	CTYRAG	5 cut(s)	137, 391, 473, 1133, 1569
SmoI	CTYRAG	5 cut(s)	137, 391, 473, 1133, 1569
SpeI	ACTAGT	1 cut(s)	521
SspI	AATATT	4 cut(s)	1579, 1897, 1927, 2519
SstI	GAGCTC	2 cut(s)	90, 3361
StyD4I	CCNGG	5 cut(s)	834, 1108, 1843, 2072, 3120
StyI	CCWWGG	1 cut(s)	2983
TaaI	ACNGT	6 cut(s)	375, 619, 1354, 2286, 3115, 3412
TaiI	ACGT	8 cut(s)	11, 1224, 1384, 2448, 2601, 3032, 3172, 3299
TatI	WGTACW	4 cut(s)	681, 2235, 2281, 3000
TauI	GCSGC	3 cut(s)	59, 62, 77
TfiI	GAWTC	7 cut(s)	1079, 1466, 1525, 1789, 2320, 2468, 2828
TscAI	CASTG	5 cut(s)	1621, 1791, 2056, 2117, 3112
TseFI	GTSAC	3 cut(s)	124, 1112, 3124
TseI	GCWGC	9 cut(s)	355, 1333, 1679, 1778, 2685, 2837, 3212, 3443, 3494
Tsp45I	GTSAC	3 cut(s)	124, 1112, 3124
TspGWI	ACGGA	5 cut(s)	1809, 1900, 2581, 2874, 3336
TspRI	CASTG	5 cut(s)	1621, 1791, 2056, 2117, 3112
Tth111I	GACNNNGTC	2 cut(s)	7, 3122
Van91I	CCANNNNNTGG	1 cut(s)	2747
Vha464I	CTTAAG	2 cut(s)	391, 1133
VpaK11BI	GGWCC	4 cut(s)	979, 1670, 1940, 2918
XagI	CCTNNNNNAGG	3 cut(s)	989, 2627, 3141
XapI	RAATTY	8 cut(s)	284, 535, 738, 1004, 2028, 2678, 3089, 3342
XbaI	TCTAGA	4 cut(s)	880, 1324, 1871, 2801
XceI	RCATGY	1 cut(s)	759
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1499
XhoI	CTCGAG	1 cut(s)	137
XmiI	GTMKAC	3 cut(s)	177, 256, 3117
XmnI	GAANNNNTTC	3 cut(s)	38, 860, 2028
ZraI	GACGTC	1 cut(s)	3297
ZrmI	AGTACT	2 cut(s)	683, 2237
Zsp2I	ATGCAT	1 cut(s)	2794

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.