FvH4_5g29520

Transmembrane emp24 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0008689
Physical Location & Seq
Reverse (-)
20466420 .. 20470745
4326 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g29520.t1

Sequence Viewer

Length: 630 bp
ATGAGGAGTGCATGCAGCTGGTTGTCGATCCTCCTATTCCTGGTTTTGTTACGGCCGGGTCAATCGATTTGGCTGACTCTACCCTCCACTGGCACCAAGTGCGTCTCCGAAGAAATCCAGAACAACGTCGTCGTTTTGGCCGATTACGTCGTTATCTCCGACGACCACTCCCACTCCCCCACCATCTCCGCCAAGGTGACGTCACCTTATGGTAATAATCTTCACCTCAGGGAGAATGCAACACATGGTCAGTTTGCCTTCACCACTCATGAGGCTGGTAACTACCTGGCATGTTTTTGGATTGATGGTCACAATCCTGGAGGCGGTTCGGTAAGTGTCAACCTTGACTGGAAAACTGGAATTGGAGCTAAGGATTGGGATTCAGTAGCGAGAAAAGAGAAGATCGAGGGTCTTGAGCTTGAGCTGCGGAAACTTGAAGGAGCAGTGGAAGCCATTCATGAAAATCTACTCTTTCTCAAGAGCAGAGAAGCGGAGATGAGGACTGTCAGTGAAAAGACAAACACCAGGGTGGCGTGGTTTAGCATAATGTCATTGGGCGTCTGCATTGCAGTTTCAAGTCTTCAGTTATGGCACTTGAAGCGATTTTTCCAAAAGAAGAAGTTGATTTGA

Protein Analysis

210

Amino Acids

23.37

Weight (kDa)

7.74

Isoelectric Point (pI)

39.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EMP24_GP25L PF01105 23 - 204 6.6e-50 emp24/gp25L/p24 family/GOLD
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 203
AccB1I GGYRCC 1 cut(s) 92
AciI CCGC 4 cut(s) 189, 324, 427, 491
AclWI GGATC 1 cut(s) 22
AcoI YGGCCR 2 cut(s) 53, 138
AcuI CTGAAG 1 cut(s) 566
AcyI GRCGYC 2 cut(s) 200, 558
AdeI CACNNNGTG 1 cut(s) 99
AfiI CCNNNNNNNGG 3 cut(s) 40, 89, 323
AgsI TTSAA 3 cut(s) 437, 576, 598
AjnI CCWGG 4 cut(s) 39, 285, 316, 524
AleI CACNNNNGTG 1 cut(s) 527
AluBI AGCT 4 cut(s) 18, 368, 418, 424
AluI AGCT 4 cut(s) 18, 368, 418, 424
Alw26I GTCTC 1 cut(s) 109
AlwI GGATC 1 cut(s) 22
AoxI GGCC 2 cut(s) 53, 138
ApeKI GCWGC 2 cut(s) 15, 424
Asp700I GAANNNNTTC 1 cut(s) 453
AsuC2I CCSGG 1 cut(s) 57
AsuHPI GGTGA 4 cut(s) 195, 208, 215, 253
AxyI CCTNAGG 1 cut(s) 227
BanI GGYRCC 1 cut(s) 92
BbsI GAAGAC 1 cut(s) 572
BbvI GCAGC 2 cut(s) 27, 411
BccI CCATC 2 cut(s) 191, 299
BceAI ACGGC 1 cut(s) 68
BciT130I CCWGG 4 cut(s) 41, 287, 318, 526
BcnI CCSGG 1 cut(s) 57
BcoDI GTCTC 1 cut(s) 109
BisI GCNGC 2 cut(s) 16, 425
BlsI GCNGC 2 cut(s) 17, 426
Bme1390I CCNGG 5 cut(s) 41, 57, 287, 318, 526
BmiI GGNNCC 1 cut(s) 94
BmrFI CCNGG 5 cut(s) 41, 57, 287, 318, 526
BpiI GAAGAC 1 cut(s) 572
BpmI CTGGAG 1 cut(s) 339
Bpu10I CCTNAGC 1 cut(s) 369
BpuEI CTTGAG 3 cut(s) 434, 440, 461
BpuMI CCSGG 1 cut(s) 57
Bsa29I ATCGAT 1 cut(s) 65
BsaHI GRCGYC 2 cut(s) 200, 558
BsaJI CCNNGG 2 cut(s) 192, 525
BsaXI ACNNNNNCTCC 4 cut(s) 152, 158, 182, 188
Bsc4I CCNNNNNNNGG 3 cut(s) 40, 89, 323
Bse1I ACTGG 3 cut(s) 94, 353, 361
Bse21I CCTNAGG 1 cut(s) 227
Bse3DI GCAATG 1 cut(s) 564
BseBI CCWGG 4 cut(s) 41, 287, 318, 526
BseCI ATCGAT 1 cut(s) 65
BseDI CCNNGG 2 cut(s) 192, 525
BseLI CCNNNNNNNGG 3 cut(s) 40, 89, 323
BseMI GCAATG 1 cut(s) 564
BseMII CTCAG 1 cut(s) 241
BseNI ACTGG 3 cut(s) 94, 353, 361
BseRI GAGGAG 1 cut(s) 19
BseX3I CGGCCG 1 cut(s) 53
BseXI GCAGC 2 cut(s) 27, 411
Bsh1285I CGRYCG 1 cut(s) 56
BshFI GGCC 2 cut(s) 55, 140
BshNI GGYRCC 1 cut(s) 92
BshVI ATCGAT 1 cut(s) 65
BsiEI CGRYCG 1 cut(s) 56
BsiSI CCGG 1 cut(s) 56
BslI CCNNNNNNNGG 3 cut(s) 40, 89, 323
BsmAI GTCTC 1 cut(s) 109
BsmBI CGTCTC 1 cut(s) 109
BsmI GAATGC 1 cut(s) 241
BsnI GGCC 2 cut(s) 55, 140
Bsp143I GATC 2 cut(s) 27, 402
BspACI CCGC 4 cut(s) 189, 324, 427, 491
BspANI GGCC 2 cut(s) 55, 140
BspCNI CTCAG 1 cut(s) 240
BspDI ATCGAT 1 cut(s) 65
BspHI TCATGA 2 cut(s) 268, 457
BspLI GGNNCC 1 cut(s) 94
BspPI GGATC 1 cut(s) 22
BspT107I GGYRCC 1 cut(s) 92
BsrDI GCAATG 1 cut(s) 564
BsrI ACTGG 3 cut(s) 94, 353, 361
BssECI CCNNGG 2 cut(s) 192, 525
BssMI GATC 2 cut(s) 27, 402
BssNI GRCGYC 2 cut(s) 200, 558
BssT1I CCWWGG 1 cut(s) 192
Bst2UI CCWGG 4 cut(s) 41, 287, 318, 526
Bst4CI ACNGT 1 cut(s) 505
BstACI GRCGYC 2 cut(s) 200, 558
BstAPI GCANNNNNTGC 1 cut(s) 99
BstC8I GCNNGC 1 cut(s) 13
BstDEI CTNAG 2 cut(s) 227, 369
BstKTI GATC 2 cut(s) 30, 405
BstMAI GTCTC 1 cut(s) 109
BstMBI GATC 2 cut(s) 27, 402
BstMCI CGRYCG 1 cut(s) 56
BstMWI GCNNNNNNNGC 4 cut(s) 99, 424, 449, 598
BstNI CCWGG 4 cut(s) 41, 287, 318, 526
BstNSI RCATGY 2 cut(s) 15, 294
BstSCI CCNGG 5 cut(s) 39, 55, 285, 316, 524
BstV1I GCAGC 2 cut(s) 27, 411
BstV2I GAAGAC 1 cut(s) 572
BstZI CGGCCG 1 cut(s) 53
Bsu15I ATCGAT 1 cut(s) 65
Bsu36I CCTNAGG 1 cut(s) 227
BsuRI GGCC 2 cut(s) 55, 140
BsuTUI ATCGAT 1 cut(s) 65
BtsI GCAGTG 1 cut(s) 450
BtsIMutI CAGTG 3 cut(s) 87, 450, 514
Cac8I GCNNGC 1 cut(s) 13
CciI TCATGA 2 cut(s) 268, 457
ClaI ATCGAT 1 cut(s) 65
CseI GACGC 2 cut(s) 91, 547
CviAII CATG 5 cut(s) 12, 245, 269, 291, 458
CviJI RGCY 9 cut(s) 18, 55, 73, 140, 275, 368, 418, 424, 452
CviKI_1 RGCY 9 cut(s) 18, 55, 73, 140, 275, 368, 418, 424, 452
DdeI CTNAG 2 cut(s) 227, 369
DpnI GATC 2 cut(s) 29, 404
DpnII GATC 2 cut(s) 27, 402
DraIII CACNNNGTG 1 cut(s) 99
EaeI YGGCCR 2 cut(s) 53, 138
EagI CGGCCG 1 cut(s) 53
EciI GGCGGA 1 cut(s) 178
EclXI CGGCCG 1 cut(s) 53
Eco130I CCWWGG 1 cut(s) 192
Eco52I CGGCCG 1 cut(s) 53
Eco57I CTGAAG 1 cut(s) 566
Eco81I CCTNAGG 1 cut(s) 227
EcoRII CCWGG 4 cut(s) 39, 285, 316, 524
EcoT14I CCWWGG 1 cut(s) 192
ErhI CCWWGG 1 cut(s) 192
Esp3I CGTCTC 1 cut(s) 109
FaeI CATG 5 cut(s) 15, 248, 272, 294, 461
FaiI YATR 8 cut(s) 13, 210, 246, 270, 292, 459, 545, 589
FatI CATG 5 cut(s) 11, 244, 268, 290, 457
Fnu4HI GCNGC 2 cut(s) 16, 425
Fsp4HI GCNGC 2 cut(s) 16, 425
GluI GCNGC 2 cut(s) 16, 425
GsuI CTGGAG 1 cut(s) 339
HaeIII GGCC 2 cut(s) 55, 140
HapII CCGG 1 cut(s) 56
HgaI GACGC 2 cut(s) 91, 547
Hin1I GRCGYC 2 cut(s) 200, 558
Hin1II CATG 5 cut(s) 15, 248, 272, 294, 461
HincII GTYRAC 1 cut(s) 340
HindII GTYRAC 1 cut(s) 340
HinfI GANTC 2 cut(s) 76, 380
HpaII CCGG 1 cut(s) 56
HphI GGTGA 4 cut(s) 195, 208, 215, 253
Hpy166II GTNNAC 1 cut(s) 340
Hpy188I TCNGA 2 cut(s) 109, 160
Hpy188III TCNNGA 5 cut(s) 118, 269, 413, 458, 478
Hpy8I GTNNAC 1 cut(s) 340
Hpy99I CGWCG 4 cut(s) 131, 134, 152, 164
HpyAV CCTTC 2 cut(s) 268, 431
HpyCH4III ACNGT 1 cut(s) 505
HpyCH4IV ACGT 3 cut(s) 126, 147, 200
HpyCH4V TGCA 5 cut(s) 11, 15, 239, 564, 569
HpyF10VI GCNNNNNNNGC 4 cut(s) 99, 424, 449, 598
HpyF3I CTNAG 2 cut(s) 227, 369
HpySE526I ACGT 3 cut(s) 126, 147, 200
Hsp92I GRCGYC 2 cut(s) 200, 558
Hsp92II CATG 5 cut(s) 15, 248, 272, 294, 461
Kzo9I GATC 2 cut(s) 27, 402
LmnI GCTCC 2 cut(s) 365, 440
Lsp1109I GCAGC 2 cut(s) 27, 411
MaeII ACGT 3 cut(s) 126, 147, 200
MaeIII GTNAC 5 cut(s) 48, 196, 201, 278, 308
MalI GATC 2 cut(s) 29, 404
MboI GATC 2 cut(s) 27, 402
MboII GAAGA 5 cut(s) 122, 212, 412, 572, 628
MluCI AATT 1 cut(s) 360
MlyI GAGTC 1 cut(s) 70
MmeI TCCRAC 1 cut(s) 183
MnlI CCTC 7 cut(s) 41, 94, 236, 265, 314, 400, 492
MroXI GAANNNNTTC 1 cut(s) 453
MslI CAYNNNNRTG 1 cut(s) 527
MspA1I CMGCKG 1 cut(s) 18
MspI CCGG 1 cut(s) 56
MspR9I CCNGG 5 cut(s) 41, 57, 287, 318, 526
Mva1269I GAATGC 1 cut(s) 241
MvaI CCWGG 4 cut(s) 41, 287, 318, 526
MwoI GCNNNNNNNGC 4 cut(s) 99, 424, 449, 598
NciI CCSGG 1 cut(s) 57
NdeII GATC 2 cut(s) 27, 402
NlaIII CATG 5 cut(s) 15, 248, 272, 294, 461
NlaIV GGNNCC 1 cut(s) 94
NmuCI GTSAC 3 cut(s) 196, 201, 308
NspI RCATGY 2 cut(s) 15, 294
OliI CACNNNNGTG 1 cut(s) 527
PaeI GCATGC 1 cut(s) 15
PagI TCATGA 2 cut(s) 268, 457
PcsI WCGNNNNNNNCGW 2 cut(s) 138, 156
PctI GAATGC 1 cut(s) 241
PdmI GAANNNNTTC 1 cut(s) 453
PfeI GAWTC 1 cut(s) 380
PfoI TCCNGGA 1 cut(s) 316
PkrI GCNGC 2 cut(s) 17, 426
PleI GAGTC 1 cut(s) 70
PpsI GAGTC 1 cut(s) 70
Psp6I CCWGG 4 cut(s) 39, 285, 316, 524
PspGI CCWGG 4 cut(s) 39, 285, 316, 524
PspN4I GGNNCC 1 cut(s) 94
PvuII CAGCTG 1 cut(s) 18
RseI CAYNNNNRTG 1 cut(s) 527
SatI GCNGC 2 cut(s) 16, 425
Sau3AI GATC 2 cut(s) 27, 402
SchI GAGTC 1 cut(s) 70
ScrFI CCNGG 5 cut(s) 41, 57, 287, 318, 526
SmiMI CAYNNNNRTG 1 cut(s) 527
SmlI CTYRAG 3 cut(s) 413, 419, 476
SmoI CTYRAG 3 cut(s) 413, 419, 476
SphI GCATGC 1 cut(s) 15
Sse9I AATT 1 cut(s) 360
SsiI CCGC 4 cut(s) 189, 324, 427, 491
StyD4I CCNGG 5 cut(s) 39, 55, 285, 316, 524
StyI CCWWGG 1 cut(s) 192
TaaI ACNGT 1 cut(s) 505
TaiI ACGT 3 cut(s) 129, 150, 203
TaqI TCGA 3 cut(s) 26, 65, 405
TasI AATT 1 cut(s) 360
TfiI GAWTC 1 cut(s) 380
TscAI CASTG 3 cut(s) 94, 450, 514
TseFI GTSAC 3 cut(s) 196, 201, 308
TseI GCWGC 2 cut(s) 15, 424
Tsp45I GTSAC 3 cut(s) 196, 201, 308
TspDTI ATGAA 2 cut(s) 446, 474
TspRI CASTG 3 cut(s) 94, 450, 514
XceI RCATGY 2 cut(s) 15, 294
XmnI GAANNNNTTC 1 cut(s) 453
ZraI GACGTC 1 cut(s) 201
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.