MD08G1229000.v1.1

Transmembrane emp24 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0008689
Physical Location & Seq
Reverse (-)
29520507 .. 29524147
3641 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1229000.v1.1.491

Sequence Viewer

Length: 648 bp
ATGGCGACGAGGAGGAGTTGGAGTGGGAGTTCGGTCGCTGCTGCCATCTTGTTGATCCTGTGCTTGGCACGGACTGGTCAAGCCATCTGGGTGACGTTACCTGCCTCCGGCACGAAATGCGTCTCGGATGAAATCCAGAACAACGTCGTCGTTTTGGCCGATTACGTCGTCATTCCGGATGATCATTCCCAAACCCCCACCATCTCCGCCAAGGTAACATCACCATATGGGAACAATCTTCATCAAAATGCGAACGCAACACACGGTCAGTTTGCATTCACAACTCATGAGGCTGGAAATTACTTGGCATGTTTTTGGGTTGATGGTAACAATCCTGGAGGTGGAGGGGTTAGTGTCAACCTTGACTGGAAAACTGGAATTGCAGCTAAGGATTGGGAATCAGTAGCAAGAAAAGAGAAAATTGAGGGTCTTGAGCTTGAGCTGCGGAAACTCGAAGGAGCAGTTGACGCCATCCATGAGAATCTACTTTATCTCAAGGGCAGAGAATCAGAGATGAGGGACGTGAGTGAAAGAACAAATGCCCGGGTGGCGTGGTTTAGCATGATATCGTTGGCTGTCTGCATTATTGTTTCGACTCTTCAGTTATGGCACTTGAAGCATTTTTTCCAAAAGAAGAAGCTTATTTAA

Protein Analysis

216

Amino Acids

23.66

Weight (kDa)

7.05

Isoelectric Point (pI)

25.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EMP24_GP25L PF01105 28 - 210 5.1e-50 emp24/gp25L/p24 family/GOLD
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 109
AccIII TCCGGA 1 cut(s) 175
AciI CCGC 2 cut(s) 207, 445
AclWI GGATC 1 cut(s) 49
AcoI YGGCCR 1 cut(s) 156
AcuI CTGAAG 1 cut(s) 584
AcyI GRCGYC 1 cut(s) 468
AfiI CCNNNNNNNGG 3 cut(s) 64, 107, 341
AgsI TTSAA 1 cut(s) 616
AjiI CACGTC 1 cut(s) 523
AjnI CCWGG 1 cut(s) 334
AloI GAACNNNNNNTCC 2 cut(s) 13, 45
AluBI AGCT 4 cut(s) 386, 436, 442, 640
AluI AGCT 4 cut(s) 386, 436, 442, 640
Alw26I GTCTC 1 cut(s) 127
AlwI GGATC 1 cut(s) 49
Ama87I CYCGRG 1 cut(s) 543
Aor13HI TCCGGA 1 cut(s) 175
AoxI GGCC 1 cut(s) 156
ApeKI GCWGC 4 cut(s) 38, 41, 383, 442
AsuC2I CCSGG 2 cut(s) 544, 545
AsuHPI GGTGA 2 cut(s) 103, 213
AvaI CYCGRG 1 cut(s) 543
BbvI GCAGC 4 cut(s) 25, 28, 395, 429
BccI CCATC 5 cut(s) 53, 92, 209, 317, 479
BciT130I CCWGG 1 cut(s) 336
BclI TGATCA 1 cut(s) 181
BcnI CCSGG 2 cut(s) 544, 545
BcoDI GTCTC 1 cut(s) 127
BfuAI ACCTGC 1 cut(s) 109
BglI GCCNNNNNGGC 1 cut(s) 548
BisI GCNGC 4 cut(s) 39, 42, 384, 443
BlsI GCNGC 4 cut(s) 40, 43, 385, 444
Bme1390I CCNGG 3 cut(s) 336, 544, 545
BmeT110I CYCGRG 1 cut(s) 543
BmgBI CACGTC 1 cut(s) 523
BmrFI CCNGG 3 cut(s) 336, 544, 545
BpmI CTGGAG 1 cut(s) 357
Bpu10I CCTNAGC 1 cut(s) 387
BpuEI CTTGAG 3 cut(s) 452, 458, 479
BpuMI CCSGG 2 cut(s) 544, 545
BsaHI GRCGYC 1 cut(s) 468
BsaJI CCNNGG 2 cut(s) 210, 543
BsaWI WCCGGW 1 cut(s) 175
BsaXI ACNNNNNCTCC 4 cut(s) 7, 13, 37, 43
Bsc4I CCNNNNNNNGG 3 cut(s) 64, 107, 341
Bse1I ACTGG 3 cut(s) 79, 371, 379
BseAI TCCGGA 1 cut(s) 175
BseBI CCWGG 1 cut(s) 336
BseDI CCNNGG 2 cut(s) 210, 543
BseGI GGATG 3 cut(s) 133, 184, 471
BseLI CCNNNNNNNGG 3 cut(s) 64, 107, 341
BseNI ACTGG 3 cut(s) 79, 371, 379
BseRI GAGGAG 2 cut(s) 25, 28
BseXI GCAGC 4 cut(s) 25, 28, 395, 429
Bsh1285I CGRYCG 1 cut(s) 36
BshFI GGCC 1 cut(s) 158
BsiEI CGRYCG 1 cut(s) 36
BsiHKCI CYCGRG 1 cut(s) 543
BsiSI CCGG 3 cut(s) 108, 176, 544
BslFI GGGAC 1 cut(s) 533
BslI CCNNNNNNNGG 3 cut(s) 64, 107, 341
BsmAI GTCTC 1 cut(s) 127
BsmBI CGTCTC 1 cut(s) 127
BsmFI GGGAC 1 cut(s) 533
BsmI GAATGC 1 cut(s) 275
BsnI GGCC 1 cut(s) 158
BsoBI CYCGRG 1 cut(s) 543
Bsp13I TCCGGA 1 cut(s) 175
Bsp143I GATC 2 cut(s) 54, 181
BspACI CCGC 2 cut(s) 207, 445
BspANI GGCC 1 cut(s) 158
BspEI TCCGGA 1 cut(s) 175
BspHI TCATGA 1 cut(s) 286
BspMI ACCTGC 1 cut(s) 109
BspPI GGATC 1 cut(s) 49
BsrI ACTGG 3 cut(s) 79, 371, 379
BssECI CCNNGG 2 cut(s) 210, 543
BssMI GATC 2 cut(s) 54, 181
BssNI GRCGYC 1 cut(s) 468
BssT1I CCWWGG 1 cut(s) 210
Bst2UI CCWGG 1 cut(s) 336
Bst4CI ACNGT 1 cut(s) 266
Bst6I CTCTTC 1 cut(s) 603
BstACI GRCGYC 1 cut(s) 468
BstAPI GCANNNNNTGC 1 cut(s) 117
BstDEI CTNAG 1 cut(s) 387
BstF5I GGATG 3 cut(s) 133, 184, 471
BstKTI GATC 2 cut(s) 57, 184
BstMAI GTCTC 1 cut(s) 127
BstMBI GATC 2 cut(s) 54, 181
BstMCI CGRYCG 1 cut(s) 36
BstMWI GCNNNNNNNGC 5 cut(s) 117, 442, 467, 548, 616
BstNI CCWGG 1 cut(s) 336
BstNSI RCATGY 1 cut(s) 312
BstSCI CCNGG 3 cut(s) 334, 542, 543
BstV1I GCAGC 4 cut(s) 25, 28, 395, 429
BsuRI GGCC 1 cut(s) 158
BtrI CACGTC 1 cut(s) 523
BtsCI GGATG 3 cut(s) 133, 184, 471
BveI ACCTGC 1 cut(s) 109
CciI TCATGA 1 cut(s) 286
Cfr9I CCCGGG 1 cut(s) 543
CseI GACGC 2 cut(s) 109, 476
CviAII CATG 4 cut(s) 287, 309, 476, 562
CviJI RGCY 8 cut(s) 83, 158, 293, 386, 436, 442, 575, 640
CviKI_1 RGCY 8 cut(s) 83, 158, 293, 386, 436, 442, 575, 640
DdeI CTNAG 1 cut(s) 387
DpnI GATC 2 cut(s) 56, 183
DpnII GATC 2 cut(s) 54, 181
EaeI YGGCCR 1 cut(s) 156
Eam1104I CTCTTC 1 cut(s) 603
EarI CTCTTC 1 cut(s) 603
EciI GGCGGA 1 cut(s) 196
Eco130I CCWWGG 1 cut(s) 210
Eco32I GATATC 1 cut(s) 567
Eco57I CTGAAG 1 cut(s) 584
Eco88I CYCGRG 1 cut(s) 543
EcoRII CCWGG 1 cut(s) 334
EcoRV GATATC 1 cut(s) 567
EcoT14I CCWWGG 1 cut(s) 210
ErhI CCWWGG 1 cut(s) 210
Esp3I CGTCTC 1 cut(s) 127
FaeI CATG 4 cut(s) 290, 312, 479, 565
FaiI YATR 7 cut(s) 226, 228, 288, 310, 477, 563, 607
FaqI GGGAC 1 cut(s) 533
FatI CATG 4 cut(s) 286, 308, 475, 561
FauNDI CATATG 1 cut(s) 226
FbaI TGATCA 1 cut(s) 181
Fnu4HI GCNGC 4 cut(s) 39, 42, 384, 443
FokI GGATG 3 cut(s) 140, 191, 458
Fsp4HI GCNGC 4 cut(s) 39, 42, 384, 443
GluI GCNGC 4 cut(s) 39, 42, 384, 443
GsuI CTGGAG 1 cut(s) 357
HaeIII GGCC 1 cut(s) 158
HapII CCGG 3 cut(s) 108, 176, 544
HgaI GACGC 2 cut(s) 109, 476
Hin1I GRCGYC 1 cut(s) 468
Hin1II CATG 4 cut(s) 290, 312, 479, 565
HincII GTYRAC 2 cut(s) 358, 466
HindII GTYRAC 2 cut(s) 358, 466
HindIII AAGCTT 1 cut(s) 638
HinfI GANTC 4 cut(s) 398, 481, 506, 595
HpaII CCGG 3 cut(s) 108, 176, 544
HphI GGTGA 2 cut(s) 103, 213
Hpy166II GTNNAC 2 cut(s) 358, 466
Hpy188I TCNGA 2 cut(s) 127, 511
Hpy188III TCNNGA 4 cut(s) 136, 176, 287, 431
Hpy8I GTNNAC 2 cut(s) 358, 466
Hpy99I CGWCG 4 cut(s) 10, 149, 152, 170
HpyAV CCTTC 1 cut(s) 449
HpyCH4III ACNGT 1 cut(s) 266
HpyCH4IV ACGT 4 cut(s) 95, 144, 165, 522
HpyCH4V TGCA 3 cut(s) 275, 383, 582
HpyF10VI GCNNNNNNNGC 5 cut(s) 117, 442, 467, 548, 616
HpyF3I CTNAG 1 cut(s) 387
HpySE526I ACGT 4 cut(s) 95, 144, 165, 522
Hsp92I GRCGYC 1 cut(s) 468
Hsp92II CATG 4 cut(s) 290, 312, 479, 565
Kpn2I TCCGGA 1 cut(s) 175
Ksp22I TGATCA 1 cut(s) 181
Kzo9I GATC 2 cut(s) 54, 181
LmnI GCTCC 1 cut(s) 458
Lsp1109I GCAGC 4 cut(s) 25, 28, 395, 429
MaeII ACGT 4 cut(s) 95, 144, 165, 522
MaeIII GTNAC 4 cut(s) 91, 96, 214, 326
MalI GATC 2 cut(s) 56, 183
MboI GATC 2 cut(s) 54, 181
MboII GAAGA 3 cut(s) 230, 590, 646
MluCI AATT 3 cut(s) 298, 378, 420
MlyI GAGTC 1 cut(s) 589
MnlI CCTC 8 cut(s) 3, 6, 115, 283, 332, 338, 418, 510
MroI TCCGGA 1 cut(s) 175
MseI TTAA 1 cut(s) 646
MslI CAYNNNNRTG 2 cut(s) 89, 246
MspI CCGG 3 cut(s) 108, 176, 544
MspR9I CCNGG 3 cut(s) 336, 544, 545
Mva1269I GAATGC 1 cut(s) 275
MvaI CCWGG 1 cut(s) 336
MwoI GCNNNNNNNGC 5 cut(s) 117, 442, 467, 548, 616
NciI CCSGG 2 cut(s) 544, 545
NdeI CATATG 1 cut(s) 226
NdeII GATC 2 cut(s) 54, 181
NlaIII CATG 4 cut(s) 290, 312, 479, 565
NmuCI GTSAC 1 cut(s) 91
NspI RCATGY 1 cut(s) 312
PagI TCATGA 1 cut(s) 286
PcsI WCGNNNNNNNCGW 1 cut(s) 156
PctI GAATGC 1 cut(s) 275
PfeI GAWTC 3 cut(s) 398, 481, 506
PfoI TCCNGGA 1 cut(s) 334
PkrI GCNGC 4 cut(s) 40, 43, 385, 444
PleI GAGTC 1 cut(s) 589
PpsI GAGTC 1 cut(s) 589
Psp6I CCWGG 1 cut(s) 334
PspGI CCWGG 1 cut(s) 334
RseI CAYNNNNRTG 2 cut(s) 89, 246
SaqAI TTAA 1 cut(s) 646
SatI GCNGC 4 cut(s) 39, 42, 384, 443
Sau3AI GATC 2 cut(s) 54, 181
SchI GAGTC 1 cut(s) 589
ScrFI CCNGG 3 cut(s) 336, 544, 545
SmaI CCCGGG 1 cut(s) 545
SmiMI CAYNNNNRTG 2 cut(s) 89, 246
SmlI CTYRAG 3 cut(s) 431, 437, 494
SmoI CTYRAG 3 cut(s) 431, 437, 494
Sse9I AATT 3 cut(s) 298, 378, 420
SsiI CCGC 2 cut(s) 207, 445
StyD4I CCNGG 3 cut(s) 334, 542, 543
StyI CCWWGG 1 cut(s) 210
TaaI ACNGT 1 cut(s) 266
TaiI ACGT 4 cut(s) 98, 147, 168, 525
TaqI TCGA 2 cut(s) 453, 593
TaqII GACCGA 1 cut(s) 22
TasI AATT 3 cut(s) 298, 378, 420
TfiI GAWTC 3 cut(s) 398, 481, 506
Tru1I TTAA 1 cut(s) 646
Tru9I TTAA 1 cut(s) 646
TseFI GTSAC 1 cut(s) 91
TseI GCWGC 4 cut(s) 38, 41, 383, 442
Tsp45I GTSAC 1 cut(s) 91
TspDTI ATGAA 2 cut(s) 144, 230
TspGWI ACGGA 1 cut(s) 85
TspMI CCCGGG 1 cut(s) 543
XceI RCATGY 1 cut(s) 312
XmaI CCCGGG 1 cut(s) 543
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.