RLG00000002056

Transmembrane emp24 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0008689
Physical Location & Seq
Reverse (-)
24811048 .. 24814500
3453 bp
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UTR
Exon/CDS
Intron
RLM00000002056

Sequence Viewer

Length: 639 bp
ATGATGATGATGATGATGAGGAGTGCGAGCTTGATCCTCCTATTCCTGTTTCTGTTGCTACGGCCGGGTCAAGCAATTTGGCTGACTCTACCCTCCAGCGGCACCAAGTGCGTCTCCGAGGAAATCCAGAACAACGTCGTCGTTTTGGCCGATTACGTCGTTATTTCCGACGACCACTCCCACTCCCCCACCATCTCCGCTAAGGTGACATCACCTTACGGCAACAATCTTCACCTCAATGAGAATGCAACACATGGTCAGTTTGCCTTCACCACTCATGAGGCTGGTAACTACCTGGCATGTTTTTGGATTGATGGTCATAATCCCGGAGGCGGTGCGGTAAGTGTCAATCTTGACTGGAAAACAGGGATTGGAGCTAAGGATTGGGAATCAGTAGCGAGAAAAGAGAAGATTGAGGGTCTTGAGCTTGAGCTGCGGAAACTTGAAGGAGCAGTGGAAGCCATCCATGAAAATCTACTCTTTCTCAAGAGCAGAGAAGCGGAGATGAGGAGTGTCAGTGAAAAGACAAACACCAGGGTGGCGTGGTTTAGCATAATGTCATTGGGCGTTTGCATTGCAGTTTCAACTCTTCAGTTATGGCACTTGAAGCAATTTTTCCAAAAGAAGAAGCTGATTTGA

Protein Analysis

213

Amino Acids

23.7

Weight (kDa)

6.71

Isoelectric Point (pI)

34.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EMP24_GP25L PF01105 25 - 207 6.4e-50 emp24/gp25L/p24 family/GOLD
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 101
AciI CCGC 6 cut(s) 99, 198, 333, 338, 436, 500
AclWI GGATC 1 cut(s) 28
AcoI YGGCCR 2 cut(s) 62, 147
AcuI CTGAAG 1 cut(s) 575
AdeI CACNNNGTG 1 cut(s) 108
AfiI CCNNNNNNNGG 2 cut(s) 98, 332
AgsI TTSAA 3 cut(s) 446, 585, 607
AjnI CCWGG 2 cut(s) 294, 533
AleI CACNNNNGTG 1 cut(s) 536
AluBI AGCT 5 cut(s) 30, 377, 427, 433, 631
AluI AGCT 5 cut(s) 30, 377, 427, 433, 631
Alw26I GTCTC 1 cut(s) 118
AlwI GGATC 1 cut(s) 28
AoxI GGCC 2 cut(s) 62, 147
ApeKI GCWGC 1 cut(s) 433
AsuC2I CCSGG 2 cut(s) 66, 327
AsuHPI GGTGA 4 cut(s) 204, 217, 224, 262
BanI GGYRCC 1 cut(s) 101
BbvI GCAGC 1 cut(s) 420
BccI CCATC 3 cut(s) 200, 308, 470
BceAI ACGGC 2 cut(s) 77, 235
BciT130I CCWGG 2 cut(s) 296, 535
BcnI CCSGG 2 cut(s) 66, 327
BcoDI GTCTC 1 cut(s) 118
BisI GCNGC 2 cut(s) 100, 434
BlsI GCNGC 2 cut(s) 101, 435
Bme1390I CCNGG 4 cut(s) 66, 296, 327, 535
BmiI GGNNCC 1 cut(s) 103
BmrFI CCNGG 4 cut(s) 66, 296, 327, 535
BpmI CTGGAG 1 cut(s) 79
Bpu10I CCTNAGC 2 cut(s) 201, 378
BpuEI CTTGAG 3 cut(s) 443, 449, 470
BpuMI CCSGG 2 cut(s) 66, 327
BsaJI CCNNGG 2 cut(s) 117, 534
BsaXI ACNNNNNCTCC 6 cut(s) 161, 167, 191, 197, 502, 532
Bsc4I CCNNNNNNNGG 2 cut(s) 98, 332
Bse1I ACTGG 1 cut(s) 362
Bse3DI GCAATG 1 cut(s) 573
BseBI CCWGG 2 cut(s) 296, 535
BseDI CCNNGG 2 cut(s) 117, 534
BseGI GGATG 1 cut(s) 462
BseLI CCNNNNNNNGG 2 cut(s) 98, 332
BseMI GCAATG 1 cut(s) 573
BseNI ACTGG 1 cut(s) 362
BseRI GAGGAG 2 cut(s) 34, 523
BseX3I CGGCCG 1 cut(s) 62
BseXI GCAGC 1 cut(s) 420
Bsh1285I CGRYCG 1 cut(s) 65
BshFI GGCC 2 cut(s) 64, 149
BshNI GGYRCC 1 cut(s) 101
BsiEI CGRYCG 1 cut(s) 65
BsiSI CCGG 2 cut(s) 65, 327
BslI CCNNNNNNNGG 2 cut(s) 98, 332
BsmAI GTCTC 1 cut(s) 118
BsmBI CGTCTC 1 cut(s) 118
BsmI GAATGC 1 cut(s) 250
BsnI GGCC 2 cut(s) 64, 149
Bsp143I GATC 1 cut(s) 33
BspACI CCGC 6 cut(s) 99, 198, 333, 338, 436, 500
BspANI GGCC 2 cut(s) 64, 149
BspHI TCATGA 1 cut(s) 277
BspLI GGNNCC 1 cut(s) 103
BspPI GGATC 1 cut(s) 28
BspT107I GGYRCC 1 cut(s) 101
BsrDI GCAATG 1 cut(s) 573
BsrI ACTGG 1 cut(s) 362
BssECI CCNNGG 2 cut(s) 117, 534
BssMI GATC 1 cut(s) 33
Bst2UI CCWGG 2 cut(s) 296, 535
Bst6I CTCTTC 1 cut(s) 594
BstAPI GCANNNNNTGC 1 cut(s) 108
BstC8I GCNNGC 1 cut(s) 28
BstDEI CTNAG 2 cut(s) 201, 378
BstF5I GGATG 1 cut(s) 462
BstKTI GATC 1 cut(s) 36
BstMAI GTCTC 1 cut(s) 118
BstMBI GATC 1 cut(s) 33
BstMCI CGRYCG 1 cut(s) 65
BstMWI GCNNNNNNNGC 4 cut(s) 108, 433, 458, 607
BstNI CCWGG 2 cut(s) 296, 535
BstNSI RCATGY 1 cut(s) 303
BstSCI CCNGG 4 cut(s) 64, 294, 325, 533
BstV1I GCAGC 1 cut(s) 420
BstZI CGGCCG 1 cut(s) 62
BsuRI GGCC 2 cut(s) 64, 149
BtsCI GGATG 1 cut(s) 462
BtsI GCAGTG 1 cut(s) 459
BtsIMutI CAGTG 2 cut(s) 459, 523
Cac8I GCNNGC 1 cut(s) 28
CciI TCATGA 1 cut(s) 277
CseI GACGC 1 cut(s) 100
CviAII CATG 4 cut(s) 254, 278, 300, 467
DdeI CTNAG 2 cut(s) 201, 378
DpnI GATC 1 cut(s) 35
DpnII GATC 1 cut(s) 33
DraIII CACNNNGTG 1 cut(s) 108
EaeI YGGCCR 2 cut(s) 62, 147
EagI CGGCCG 1 cut(s) 62
Eam1104I CTCTTC 1 cut(s) 594
EarI CTCTTC 1 cut(s) 594
EclXI CGGCCG 1 cut(s) 62
Eco52I CGGCCG 1 cut(s) 62
Eco57I CTGAAG 1 cut(s) 575
EcoRII CCWGG 2 cut(s) 294, 533
Esp3I CGTCTC 1 cut(s) 118
FaeI CATG 4 cut(s) 257, 281, 303, 470
FaiI YATR 7 cut(s) 255, 279, 301, 321, 468, 554, 598
FatI CATG 4 cut(s) 253, 277, 299, 466
Fnu4HI GCNGC 2 cut(s) 100, 434
FokI GGATG 1 cut(s) 449
Fsp4HI GCNGC 2 cut(s) 100, 434
GluI GCNGC 2 cut(s) 100, 434
GsuI CTGGAG 1 cut(s) 79
HaeIII GGCC 2 cut(s) 64, 149
HapII CCGG 2 cut(s) 65, 327
HgaI GACGC 1 cut(s) 100
Hin1II CATG 4 cut(s) 257, 281, 303, 470
HinfI GANTC 2 cut(s) 85, 389
HpaII CCGG 2 cut(s) 65, 327
HphI GGTGA 4 cut(s) 204, 217, 224, 262
Hpy188I TCNGA 2 cut(s) 118, 169
Hpy188III TCNNGA 5 cut(s) 127, 278, 353, 422, 487
Hpy99I CGWCG 4 cut(s) 140, 143, 161, 173
HpyAV CCTTC 2 cut(s) 277, 440
HpyCH4IV ACGT 2 cut(s) 135, 156
HpyCH4V TGCA 3 cut(s) 248, 573, 578
HpyF10VI GCNNNNNNNGC 4 cut(s) 108, 433, 458, 607
HpyF3I CTNAG 2 cut(s) 201, 378
HpySE526I ACGT 2 cut(s) 135, 156
Hsp92II CATG 4 cut(s) 257, 281, 303, 470
Kzo9I GATC 1 cut(s) 33
LmnI GCTCC 2 cut(s) 374, 449
Lsp1109I GCAGC 1 cut(s) 420
MaeII ACGT 2 cut(s) 135, 156
MaeIII GTNAC 2 cut(s) 205, 287
MalI GATC 1 cut(s) 35
MboI GATC 1 cut(s) 33
MboII GAAGA 4 cut(s) 221, 421, 581, 637
MluCI AATT 2 cut(s) 75, 611
MlyI GAGTC 1 cut(s) 79
MmeI TCCRAC 1 cut(s) 192
MnlI CCTC 9 cut(s) 12, 47, 103, 112, 245, 274, 323, 409, 501
MslI CAYNNNNRTG 2 cut(s) 237, 536
MspA1I CMGCKG 1 cut(s) 99
MspI CCGG 2 cut(s) 65, 327
MspR9I CCNGG 4 cut(s) 66, 296, 327, 535
Mva1269I GAATGC 1 cut(s) 250
MvaI CCWGG 2 cut(s) 296, 535
MwoI GCNNNNNNNGC 4 cut(s) 108, 433, 458, 607
NciI CCSGG 2 cut(s) 66, 327
NdeII GATC 1 cut(s) 33
NlaIII CATG 4 cut(s) 257, 281, 303, 470
NlaIV GGNNCC 1 cut(s) 103
NmuCI GTSAC 1 cut(s) 205
NspI RCATGY 1 cut(s) 303
OliI CACNNNNGTG 1 cut(s) 536
PagI TCATGA 1 cut(s) 277
PcsI WCGNNNNNNNCGW 2 cut(s) 147, 165
PctI GAATGC 1 cut(s) 250
PfeI GAWTC 1 cut(s) 389
PfoI TCCNGGA 1 cut(s) 325
PkrI GCNGC 2 cut(s) 101, 435
PleI GAGTC 1 cut(s) 79
PpsI GAGTC 1 cut(s) 79
Psp6I CCWGG 2 cut(s) 294, 533
PspGI CCWGG 2 cut(s) 294, 533
PspN4I GGNNCC 1 cut(s) 103
RseI CAYNNNNRTG 2 cut(s) 237, 536
SatI GCNGC 2 cut(s) 100, 434
Sau3AI GATC 1 cut(s) 33
SchI GAGTC 1 cut(s) 79
ScrFI CCNGG 4 cut(s) 66, 296, 327, 535
SmiMI CAYNNNNRTG 2 cut(s) 237, 536
SmlI CTYRAG 3 cut(s) 422, 428, 485
SmoI CTYRAG 3 cut(s) 422, 428, 485
Sse9I AATT 2 cut(s) 75, 611
SsiI CCGC 6 cut(s) 99, 198, 333, 338, 436, 500
StyD4I CCNGG 4 cut(s) 64, 294, 325, 533
TaiI ACGT 2 cut(s) 138, 159
TasI AATT 2 cut(s) 75, 611
TauI GCSGC 1 cut(s) 102
TfiI GAWTC 1 cut(s) 389
TscAI CASTG 2 cut(s) 459, 523
TseFI GTSAC 1 cut(s) 205
TseI GCWGC 1 cut(s) 433
Tsp45I GTSAC 1 cut(s) 205
TspDTI ATGAA 1 cut(s) 483
TspRI CASTG 2 cut(s) 459, 523
XceI RCATGY 1 cut(s) 303
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.