FvH4_5g29900

FES

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0007319
Physical Location & Seq
Forward (+)
20769390 .. 20781080
11691 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g29900.t1

Sequence Viewer

Length: 3357 bp
ATGACGAAGGCACCCAGAGCAAAGGATGTCATGGACTCCTCTGTTCATACTCCCGTAAGGCCTCAGACTATGAAGTTTTACCAGAGGAAAAACACAGCAAAGGATGATATTGTCATGGACTCCTCTGTACATACCCCCGTTAGGCCTCAGACTATGAAGTTTTACCAGAGGAAAAACAGAGCAAAGGACAACATTGTCATGGACTCCTCTGTTCATACCCCCGTAAGGCCTCAGACTATGAAGGTTTACCAGAGGAAAAACAGAGCAAAGGACGACATTGTCTCCTCTGTTCATAACCCCGTAAAGCCTCAGACTATGAAGGTTTACCAGAGGAAAAGGAAAAACAAAGCGAAGGTAGGCGGTGATATTCCGGTAAAGACTCGGGCTATCAGAATCCCTGAAAGTGGCAGCATTGAGGGTTCTTTCATGCCAGTACCTTCACAACCCGAATCCATGTCACAATATAACTTGAAGAATGGAGAATTGGTTGATGGCAAGTTCTGTATTGGTGATACAAGCAATATTAGTATATTAAAAAGAAAACTACATGATGCTACACTTCAAACGCCACAAAAGAGACTCAGGAGACCAAACAAAAAAGTTTACCGGCCAAAAGTGGTACCTAATCTAGGAATGTCTGTGCCTAGAATGGAACTGAAGACTCCCACCAAAGAGACACCAAAAAGGATGAATAATGTGTCATTGAAGAAGAGTTTTATTACAATCGATCTGCAAAAAATACAAAAGAAAAAAAGGCTGGGCAGGAGGAGAATGGAAGTAGAGAGGTGGCAGAAGATAGCTATAAAGTTTCAAGCAAACTATCTTGAACAGAAATATAATAAGAGAAGAGCTGCCCCCGGAATCAGTCAAAAGGAATTGCAGAAACTTTTTGACAACGAACAGCAGTTGTCTCCATGTTTCATCACCCAATCAGGAGATTTGAACATTGAAGAAAGCTTATCCGGAATAGTAAGCCTACTGGAAAGTGATGATTTGGAGCTGTTGGTCAAAAAAATAGCTCATGCAAATGGTGAACGAGGTATGGTGGCCATGACAAAGAAAAAAGGACGCTGGCAAGAAAAGCTTGAGAAATTAGAACACGGTGAACTTTTTCATCCATGGAATCTTCAATTGCAAAATATAGTTAAGGAGGGGGATATACACGATGAGGAGCATTCTCAGTTTTGGGAGAAGGAGAGAGAATTATTTCGTGGTCGGATTGACTTATTTCTGACTCGTATGCGTGATATTCAAGGAAATAGAACATTTTCAGAATGGGATGGTTCAGTTGTAGATTCGATAGTTGGAGTTTTTTTGACACAGAACGTATCAGATCATCTATCCAGCTCTGCGTTTATTTCACTAGCTGCAGCCTTCCCATGCCAGCCCACTTGTGATAAGCAATCAAAAATTGTTAAAATTGAGGAGTACATAGAGGGTGGAACCTCGAAGTCAAGCTGTGAACCATCAAATGACCAAACATATGGTGAAGATATAAATATTGCCAGCTCTACAAGTAACTTGGTGTTATATGATGAGTCTGAGTGCTTGGTGTTATATGATGAGTCTGAGTGCTGGGATGATATTATAAGTAACAACTCGCAGACTCACTTTAATGCGATAAAACAAAAAGGAAAGAAGTTGAAGTTGAAAGCAAAACAAAAAGGAAAGAAGTTGAAGGCGGATGCTGAAGAAATAAACAATATTGAGGGTGCTTTGGTGAATGAAACACCTGGTTGGGATTATTATAAAAAAAAAATAGAGCTTAATTGTGAGGACAAAAGAATGTTGCAGCCCAGAGATTCGGTGAATTGGCAGGCCGTTAGACAAGCAGATGTTGATGACATTGCTGAAGTCATAAAGGGACGTGGACAACAACATAACATCGCAGATCGAATAAAGCAATTTCTTAATGAAGTGAATGAAGACCATAAAGAAATAAACCTTGAGTGGCTACGATGGGCTCCGGCAGAACAAGCAAGGACATACTTGACTACTATTAAGGGAATCGGTTTGAAGAGTGTAGAGTGTGTGCGCCTTTTAGCACTCAAGCAGATTGCATTCCCAGTGGACGTTAATGTTGCCAGGATAGCCGTTCGTTTAGGGTGGGTTCCTTTGCAACCATTACCTGAAGATGTTCAGATACACGTATTAGAAAATATGCCAGTTGTAGACGATATTCAAAAGTATCTTTGGCCACGGCTAAAGAAGTTGCGGGGCTTCAAGGAATTGTATGAACTGCACTATCACTTGATAACTTTTGGAAAGGTATATTGTACCAAACGTAAGCCTAACTGTCACTCATGTCCAATGAAGAACGAGTGCAAGTATTATCTCAGCGCGTACGCAAGTGCAAAACATGCTGCGTTACCAAAGTCAACGGAGACACAAAGGCAAATGAAAAGAGATGAAAATAATAAAATCGTTTGCGCTAGTCATCTTTCTTCTAGATGTGAGCCACCAAATCCAACATCACATACATTTATTGAGGACACTACCAAATTAGAATATAAATTGAGAGAATGTGAACCAATAATTGAAGAACCCGCATCACCTGGAAGGTCATCACCGGAACCAGAATATGTGGAAAGAGACATCGAAGACTTATTCACTGACGAGTCTGTAACAAGAAGTCCACATATGAATGCATTTGGAGGAAGTGATATTTCATCACTAGTACCCTTTATGACCCGTGAAGAAGCTTTTTCAATCCCAGCTCGCAGACTAAAAGAAATTGAGCGTTTGAGGACAAAACACTCGGTATACAAGATTGAAAAAGAAAGCATTTTATTCAAAGAGCTGAAACTAGAGAGACGAGACCCTAGAGATAAATATCCTTACCTTTTTGCGATGTGCAAACCAACACCAGTATCTCAACCACATGAAGACAGATGCAGTTGCAATGGGTCTAAAGATTCATCATGTGATGGTGAGGAGACTTGTTTTTCTTGTAGCAGTCTCTCAACACAAGATGACACTGATACTATTCGAGGAACAATTTTGATACCTTGTAGAACAGCAACGAGGGGAAGTTTTCCTTTAAATGGTACATATTTCCAGATTAATGAGGTTTTTGCAGATCACGAGTCTAGCATAAACCCCATTAAAGTTCATAGAGACTTGTTATGGAATCAGACGACAAGAGATGTATACTGTGGAACCTCAGCAAGTCATATTCTCAAAGGCCTATCAAGAGATGAACTTAGTGAGTGCTTCAAGGATGGTTTTATTTGTGTGAGAGCATTCAATCGAAGAACACGTACGCCTGAGCCTCTTCATGAAAGGCTTCACATTTCAACTGTCAAAACAGCAAAGAAAGGTAAGAAGAAATTGGAACCGGAGGAGGATGATGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Perm-CXXC RRM_DME

Protein Analysis

1119

Amino Acids

128.54

Weight (kDa)

9.04

Isoelectric Point (pI)

54.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_DME PF15628 998 - 1098 1e-46 RRM in Demeter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1589, 1749
AasI GACNNNNNNGTC 2 cut(s) 194, 278
Acc65I GGTACC 1 cut(s) 619
AccB1I GGYRCC 2 cut(s) 10, 619
AccI GTMKAC 3 cut(s) 2172, 2763, 3150
AccII CGCG 1 cut(s) 2342
AccIII TCCGGA 1 cut(s) 962
AciI CCGC 4 cut(s) 360, 1682, 2215, 2547
AcoI YGGCCR 3 cut(s) 608, 1047, 2195
AcuI CTGAAG 4 cut(s) 677, 1710, 1872, 2151
AfaI GTAC 9 cut(s) 129, 435, 621, 1430, 2278, 2345, 2679, 3049, 3262
AfiI CCNNNNNNNGG 5 cut(s) 141, 225, 404, 629, 3044
AflIII ACRYGT 2 cut(s) 2146, 3257
AhlI ACTAGT 1 cut(s) 2674
AjiI CACGTC 1 cut(s) 1868
AjnI CCWGG 3 cut(s) 1732, 2084, 2554
AjuI GAANNNNNNNTTGG 2 cut(s) 467, 499
Ama87I CYCGRG 1 cut(s) 381
Aor13HI TCCGGA 1 cut(s) 962
AoxI GGCC 8 cut(s) 59, 143, 227, 608, 1047, 1818, 2195, 3184
ApeKI GCWGC 6 cut(s) 408, 851, 1367, 1370, 1792, 2363
AseI ATTAAT 1 cut(s) 3063
Asp700I GAANNNNTTC 5 cut(s) 1110, 1206, 1267, 2136, 3285
Asp718I GGTACC 1 cut(s) 619
AspLEI GCGC 3 cut(s) 2037, 2342, 2432
AsuC2I CCSGG 1 cut(s) 858
AvaI CYCGRG 1 cut(s) 381
BaeI ACNNNNGTAYC 2 cut(s) 2853, 2886
BalI TGGCCA 2 cut(s) 1049, 2197
BanI GGYRCC 2 cut(s) 10, 619
BanII GRGCYC 1 cut(s) 1966
BarI GAAGNNNNNNTAC 2 cut(s) 3244, 3276
BauI CACGAG 1 cut(s) 3083
BbsI GAAGAC 4 cut(s) 665, 1932, 2607, 2892
BbvCI CCTCAGC 1 cut(s) 3163
BbvI GCAGC 6 cut(s) 420, 838, 1354, 1382, 1804, 2350
BccI CCATC 6 cut(s) 485, 1274, 1474, 1953, 2921, 3215
BceAI ACGGC 3 cut(s) 1805, 2078, 2216
BciT130I CCWGG 3 cut(s) 1734, 2086, 2556
BcnI CCSGG 1 cut(s) 858
BcuI ACTAGT 1 cut(s) 2674
BfaI CTAG 9 cut(s) 629, 645, 1364, 2433, 2448, 2675, 2807, 2823, 3090
BfmI CTRYAG 1 cut(s) 1368
BisI GCNGC 6 cut(s) 409, 852, 1368, 1371, 1793, 2364
BlsI GCNGC 6 cut(s) 410, 853, 1369, 1372, 1794, 2365
Bme1390I CCNGG 4 cut(s) 858, 1734, 2086, 2556
BmeT110I CYCGRG 1 cut(s) 381
BmgBI CACGTC 1 cut(s) 1868
BmiI GGNNCC 8 cut(s) 12, 621, 1444, 1965, 2112, 2574, 3160, 3336
BmrFI CCNGG 4 cut(s) 858, 1734, 2086, 2556
BmrI ACTGGG 1 cut(s) 2060
BmsI GCATC 4 cut(s) 541, 1675, 2558, 2882
BmuI ACTGGG 1 cut(s) 2060
BpiI GAAGAC 4 cut(s) 665, 1932, 2607, 2892
Bpu10I CCTNAGC 2 cut(s) 3163, 3267
BpuEI CTTGAG 3 cut(s) 1106, 1967, 2033
BpuMI CCSGG 1 cut(s) 858
Bsa29I ATCGAT 1 cut(s) 726
BsaAI YACGTR 2 cut(s) 2149, 3260
BsaBI GATNNNNATC 1 cut(s) 2832
BsaI GGTCTC 2 cut(s) 580, 2811
BsaJI CCNNGG 3 cut(s) 856, 1118, 2198
BsaWI WCCGGW 4 cut(s) 370, 962, 2569, 3337
BsaXI ACNNNNNCTCC 4 cut(s) 266, 296, 471, 501
Bsc4I CCNNNNNNNGG 5 cut(s) 141, 225, 404, 629, 3044
Bse118I RCCGGY 1 cut(s) 606
Bse1I ACTGG 5 cut(s) 431, 984, 2066, 2165, 2867
Bse3DI GCAATG 2 cut(s) 1845, 2908
Bse8I GATNNNNATC 1 cut(s) 2832
BseAI TCCGGA 1 cut(s) 962
BseBI CCWGG 3 cut(s) 1734, 2086, 2556
BseCI ATCGAT 1 cut(s) 726
BseDI CCNNGG 3 cut(s) 856, 1118, 2198
BseGI GGATG 9 cut(s) 31, 109, 693, 1114, 1285, 1585, 1690, 3226, 3352
BseJI GATNNNNATC 1 cut(s) 2832
BseLI CCNNNNNNNGG 5 cut(s) 141, 225, 404, 629, 3044
BseMI GCAATG 2 cut(s) 1845, 2908
BseNI ACTGG 5 cut(s) 431, 984, 2066, 2165, 2867
BseRI GAGGAG 9 cut(s) 28, 112, 196, 274, 781, 1184, 1439, 2948, 3356
BseXI GCAGC 6 cut(s) 420, 838, 1354, 1382, 1804, 2350
BseYI CCCAGC 3 cut(s) 757, 1575, 2713
BsgI GTGCAG 1 cut(s) 2225
Bsh1236I CGCG 1 cut(s) 2342
BshFI GGCC 8 cut(s) 61, 145, 229, 610, 1049, 1820, 2197, 3186
BshNI GGYRCC 2 cut(s) 10, 619
BshVI ATCGAT 1 cut(s) 726
BsiHKCI CYCGRG 1 cut(s) 381
BsiSI CCGG 7 cut(s) 371, 607, 858, 963, 1967, 2570, 3338
BsiWI CGTACG 2 cut(s) 2343, 3260
BslFI GGGAC 1 cut(s) 1878
BslI CCNNNNNNNGG 5 cut(s) 141, 225, 404, 629, 3044
BsmBI CGTCTC 1 cut(s) 2806
BsmFI GGGAC 1 cut(s) 1878
BsmI GAATGC 4 cut(s) 1174, 2060, 2650, 3242
BsnI GGCC 8 cut(s) 61, 145, 229, 610, 1049, 1820, 2197, 3186
Bso31I GGTCTC 2 cut(s) 580, 2811
BsoBI CYCGRG 1 cut(s) 381
Bsp1286I GDGCHC 1 cut(s) 1966
Bsp13I TCCGGA 1 cut(s) 962
Bsp1407I TGTACA 1 cut(s) 127
Bsp143I GATC 4 cut(s) 727, 1333, 1891, 3079
Bsp19I CCATGG 1 cut(s) 1118
BspACI CCGC 4 cut(s) 360, 1682, 2215, 2547
BspANI GGCC 8 cut(s) 61, 145, 229, 610, 1049, 1820, 2197, 3186
BspDI ATCGAT 1 cut(s) 726
BspEI TCCGGA 1 cut(s) 962
BspFNI CGCG 1 cut(s) 2342
BspHI TCATGA 1 cut(s) 3277
BspLI GGNNCC 8 cut(s) 12, 621, 1444, 1965, 2112, 2574, 3160, 3336
BspMAI CTGCAG 1 cut(s) 1372
BspQI GCTCTTC 1 cut(s) 841
BspT107I GGYRCC 2 cut(s) 10, 619
BspTNI GGTCTC 2 cut(s) 580, 2811
BsrDI GCAATG 2 cut(s) 1845, 2908
BsrFI RCCGGY 1 cut(s) 606
BsrGI TGTACA 1 cut(s) 127
BsrI ACTGG 5 cut(s) 431, 984, 2066, 2165, 2867
BssAI RCCGGY 1 cut(s) 606
BssECI CCNNGG 3 cut(s) 856, 1118, 2198
BssMI GATC 4 cut(s) 727, 1333, 1891, 3079
BssNAI GTATAC 2 cut(s) 2764, 3151
BssSI CACGAG 1 cut(s) 3083
BssT1I CCWWGG 1 cut(s) 1118
Bst1107I GTATAC 2 cut(s) 2764, 3151
Bst2BI CACGAG 1 cut(s) 3083
Bst2UI CCWGG 3 cut(s) 1734, 2086, 2556
Bst4CI ACNGT 4 cut(s) 1103, 2297, 3155, 3301
Bst6I CTCTTC 4 cut(s) 704, 841, 2012, 3279
BstAPI GCANNNNNTGC 1 cut(s) 2360
BstAUI TGTACA 1 cut(s) 127
BstBAI YACGTR 2 cut(s) 2149, 3260
BstC8I GCNNGC 5 cut(s) 1073, 1385, 1507, 1818, 2719
BstDSI CCRYGG 2 cut(s) 1118, 2198
BstENI CCTNNNNNAGG 1 cut(s) 627
BstF5I GGATG 9 cut(s) 31, 109, 693, 1114, 1285, 1585, 1690, 3226, 3352
BstFNI CGCG 1 cut(s) 2342
BstHHI GCGC 3 cut(s) 2037, 2342, 2432
BstKTI GATC 4 cut(s) 730, 1336, 1894, 3082
BstMBI GATC 4 cut(s) 727, 1333, 1891, 3079
BstMWI GCNNNNNNNGC 5 cut(s) 17, 1081, 1976, 2090, 2360
BstNI CCWGG 3 cut(s) 1734, 2086, 2556
BstNSI RCATGY 1 cut(s) 2363
BstSCI CCNGG 4 cut(s) 856, 1732, 2084, 2554
BstSFI CTRYAG 1 cut(s) 1368
BstUI CGCG 1 cut(s) 2342
BstV1I GCAGC 6 cut(s) 420, 838, 1354, 1382, 1804, 2350
BstV2I GAAGAC 4 cut(s) 665, 1932, 2607, 2892
BstXI CCANNNNNNTGG 1 cut(s) 1484
BstZ17I GTATAC 2 cut(s) 2764, 3151
Bsu15I ATCGAT 1 cut(s) 726
BsuRI GGCC 8 cut(s) 61, 145, 229, 610, 1049, 1820, 2197, 3186
BsuTUI ATCGAT 1 cut(s) 726
BtgI CCRYGG 2 cut(s) 1118, 2198
BtgZI GCGATG 2 cut(s) 1870, 2864
BtrI CACGTC 1 cut(s) 1868
BtsCI GGATG 9 cut(s) 31, 109, 693, 1114, 1285, 1585, 1690, 3226, 3352
BtsIMutI CAGTG 3 cut(s) 2073, 2610, 2976
Cac8I GCNNGC 5 cut(s) 1073, 1385, 1507, 1818, 2719
CciI TCATGA 1 cut(s) 3277
CfoI GCGC 3 cut(s) 2037, 2342, 2432
Cfr10I RCCGGY 1 cut(s) 606
ClaI ATCGAT 1 cut(s) 726
CseI GACGC 1 cut(s) 1077
CsiI ACCWGGT 1 cut(s) 1732
Csp6I GTAC 9 cut(s) 128, 434, 620, 1429, 2277, 2344, 2678, 3048, 3261
CviQI GTAC 9 cut(s) 128, 434, 620, 1429, 2277, 2344, 2678, 3048, 3261
DpnI GATC 4 cut(s) 729, 1335, 1893, 3081
DpnII GATC 4 cut(s) 727, 1333, 1891, 3079
DraI TTTAAA 1 cut(s) 3042
DrdI GACNNNNNNGTC 2 cut(s) 194, 278
DseDI GACNNNNNNGTC 2 cut(s) 194, 278
EaeI YGGCCR 3 cut(s) 608, 1047, 2195
Eam1104I CTCTTC 4 cut(s) 704, 841, 2012, 3279
EarI CTCTTC 4 cut(s) 704, 841, 2012, 3279
EciI GGCGGA 1 cut(s) 1697
Eco130I CCWWGG 1 cut(s) 1118
Eco147I AGGCCT 4 cut(s) 61, 145, 229, 3186
Eco24I GRGCYC 1 cut(s) 1966
Eco31I GGTCTC 2 cut(s) 580, 2811
Eco57I CTGAAG 4 cut(s) 677, 1710, 1872, 2151
Eco88I CYCGRG 1 cut(s) 381
EcoNI CCTNNNNNAGG 1 cut(s) 627
EcoRII CCWGG 3 cut(s) 1732, 2084, 2554
EcoT14I CCWWGG 1 cut(s) 1118
EcoT22I ATGCAT 1 cut(s) 2650
EcoT38I GRGCYC 1 cut(s) 1966
ErhI CCWWGG 1 cut(s) 1118
Esp3I CGTCTC 1 cut(s) 2806
FalI AAGNNNNNCTT 4 cut(s) 1068, 1100, 3022, 3054
FaqI GGGAC 1 cut(s) 1878
FauI CCCGC 2 cut(s) 2208, 2554
FauNDI CATATG 2 cut(s) 1483, 2640
FblI GTMKAC 3 cut(s) 2172, 2763, 3150
Fnu4HI GCNGC 6 cut(s) 409, 852, 1368, 1371, 1793, 2364
FokI GGATG 8 cut(s) 38, 116, 700, 1101, 1292, 1592, 1697, 3233
FriOI GRGCYC 1 cut(s) 1966
Fsp4HI GCNGC 6 cut(s) 409, 852, 1368, 1371, 1793, 2364
FspBI CTAG 9 cut(s) 629, 645, 1364, 2433, 2448, 2675, 2807, 2823, 3090
GlaI GCGC 3 cut(s) 2036, 2341, 2431
GluI GCNGC 6 cut(s) 409, 852, 1368, 1371, 1793, 2364
GsaI CCCAGC 3 cut(s) 761, 1579, 2717
HaeIII GGCC 8 cut(s) 61, 145, 229, 610, 1049, 1820, 2197, 3186
HapII CCGG 7 cut(s) 371, 607, 858, 963, 1967, 2570, 3338
HgaI GACGC 1 cut(s) 1077
HhaI GCGC 3 cut(s) 2037, 2342, 2432
Hin6I GCGC 3 cut(s) 2035, 2340, 2430
HinP1I GCGC 3 cut(s) 2035, 2340, 2430
HincII GTYRAC 1 cut(s) 2379
HindII GTYRAC 1 cut(s) 2379
HindIII AAGCTT 3 cut(s) 955, 1082, 2700
HpaII CCGG 7 cut(s) 371, 607, 858, 963, 1967, 2570, 3338
Hpy188III TCNNGA 9 cut(s) 583, 824, 933, 963, 2448, 3058, 3083, 3192, 3278
HpyAV CCTTC 8 cut(s) 235, 313, 346, 447, 1186, 1384, 1672, 2553
HpyCH4III ACNGT 4 cut(s) 1103, 2297, 3155, 3301
HpyCH4IV ACGT 6 cut(s) 1326, 1867, 2073, 2148, 2284, 3259
HpyF10VI GCNNNNNNNGC 5 cut(s) 17, 1081, 1976, 2090, 2360
HpySE526I ACGT 6 cut(s) 1326, 1867, 2073, 2148, 2284, 3259
HspAI GCGC 3 cut(s) 2035, 2340, 2430
Kpn2I TCCGGA 1 cut(s) 962
KpnI GGTACC 1 cut(s) 623
Kzo9I GATC 4 cut(s) 727, 1333, 1891, 3079
LguI GCTCTTC 1 cut(s) 841
LmnI GCTCC 3 cut(s) 997, 1171, 1969
Lsp1109I GCAGC 6 cut(s) 420, 838, 1354, 1382, 1804, 2350
LweI GCATC 4 cut(s) 541, 1675, 2558, 2882
MabI ACCWGGT 1 cut(s) 1732
MaeI CTAG 9 cut(s) 629, 645, 1364, 2433, 2448, 2675, 2807, 2823, 3090
MaeII ACGT 6 cut(s) 1326, 1867, 2073, 2148, 2284, 3259
MaeIII GTNAC 6 cut(s) 456, 1517, 1592, 2297, 2367, 2623
MalI GATC 4 cut(s) 729, 1335, 1893, 3081
MboI GATC 4 cut(s) 727, 1333, 1891, 3079
MfeI CAATTG 1 cut(s) 1130
MhlI GDGCHC 1 cut(s) 1966
MlsI TGGCCA 2 cut(s) 1049, 2197
MluNI TGGCCA 2 cut(s) 1049, 2197
MmeI TCCRAC 3 cut(s) 1196, 1285, 2492
Mox20I TGGCCA 2 cut(s) 1049, 2197
Mph1103I ATGCAT 1 cut(s) 2650
MroI TCCGGA 1 cut(s) 962
MroXI GAANNNNTTC 5 cut(s) 1110, 1206, 1267, 2136, 3285
MscI TGGCCA 2 cut(s) 1049, 2197
MslI CAYNNNNRTG 4 cut(s) 197, 1026, 1614, 2643
Msp20I TGGCCA 2 cut(s) 1049, 2197
MspI CCGG 7 cut(s) 371, 607, 858, 963, 1967, 2570, 3338
MspR9I CCNGG 4 cut(s) 858, 1734, 2086, 2556
MunI CAATTG 1 cut(s) 1130
Mva1269I GAATGC 4 cut(s) 1174, 2060, 2650, 3242
MvaI CCWGG 3 cut(s) 1734, 2086, 2556
MvnI CGCG 1 cut(s) 2342
MwoI GCNNNNNNNGC 5 cut(s) 17, 1081, 1976, 2090, 2360
NciI CCSGG 1 cut(s) 858
NcoI CCATGG 1 cut(s) 1118
NdeI CATATG 2 cut(s) 1483, 2640
NdeII GATC 4 cut(s) 727, 1333, 1891, 3079
NlaIV GGNNCC 8 cut(s) 12, 621, 1444, 1965, 2112, 2574, 3160, 3336
NmuCI GTSAC 2 cut(s) 456, 2297
NsiI ATGCAT 1 cut(s) 2650
NspI RCATGY 1 cut(s) 2363
PagI TCATGA 1 cut(s) 3277
PceI AGGCCT 4 cut(s) 61, 145, 229, 3186
PciSI GCTCTTC 1 cut(s) 841
PcsI WCGNNNNNNNCGW 1 cut(s) 3256
PctI GAATGC 4 cut(s) 1174, 2060, 2650, 3242
PdmI GAANNNNTTC 5 cut(s) 1110, 1206, 1267, 2136, 3285
PfeI GAWTC 9 cut(s) 393, 449, 861, 1123, 1295, 1802, 2007, 2915, 3130
Pfl23II CGTACG 2 cut(s) 2343, 3260
PflFI GACNNNGTC 1 cut(s) 278
PkrI GCNGC 6 cut(s) 410, 853, 1369, 1372, 1794, 2365
Ppu21I YACGTR 2 cut(s) 2149, 3260
PshBI ATTAAT 1 cut(s) 3063
PsiI TTATAA 2 cut(s) 1589, 1749
Psp6I CCWGG 3 cut(s) 1732, 2084, 2554
PspFI CCCAGC 3 cut(s) 757, 1575, 2713
PspGI CCWGG 3 cut(s) 1732, 2084, 2554
PspLI CGTACG 2 cut(s) 2343, 3260
PspN4I GGNNCC 8 cut(s) 12, 621, 1444, 1965, 2112, 2574, 3160, 3336
PstI CTGCAG 1 cut(s) 1372
PsyI GACNNNGTC 1 cut(s) 278
RsaI GTAC 9 cut(s) 129, 435, 621, 1430, 2278, 2345, 2679, 3049, 3262
RsaNI GTAC 9 cut(s) 128, 434, 620, 1429, 2277, 2344, 2678, 3048, 3261
RseI CAYNNNNRTG 4 cut(s) 197, 1026, 1614, 2643
SapI GCTCTTC 1 cut(s) 841
SatI GCNGC 6 cut(s) 409, 852, 1368, 1371, 1793, 2364
Sau3AI GATC 4 cut(s) 727, 1333, 1891, 3079
ScrFI CCNGG 4 cut(s) 858, 1734, 2086, 2556
SduI GDGCHC 1 cut(s) 1966
SexAI ACCWGGT 1 cut(s) 1732
SfaNI GCATC 4 cut(s) 541, 1675, 2558, 2882
SfcI CTRYAG 1 cut(s) 1368
SmiMI CAYNNNNRTG 4 cut(s) 197, 1026, 1614, 2643
SmlI CTYRAG 3 cut(s) 1085, 1946, 2048
SmoI CTYRAG 3 cut(s) 1085, 1946, 2048
SpeI ACTAGT 1 cut(s) 2674
SseBI AGGCCT 4 cut(s) 61, 145, 229, 3186
SsiI CCGC 4 cut(s) 360, 1682, 2215, 2547
SspI AATATT 3 cut(s) 523, 1501, 1705
SspMI CTAG 9 cut(s) 629, 645, 1364, 2433, 2448, 2675, 2807, 2823, 3090
StuI AGGCCT 4 cut(s) 61, 145, 229, 3186
StyD4I CCNGG 4 cut(s) 856, 1732, 2084, 2554
StyI CCWWGG 1 cut(s) 1118
TaaI ACNGT 4 cut(s) 1103, 2297, 3155, 3301
TaiI ACGT 6 cut(s) 1329, 1870, 2076, 2151, 2287, 3262
TaqI TCGA 7 cut(s) 726, 1298, 1448, 1894, 2598, 2989, 3250
TatI WGTACW 2 cut(s) 127, 1428
TfiI GAWTC 9 cut(s) 393, 449, 861, 1123, 1295, 1802, 2007, 2915, 3130
TscAI CASTG 3 cut(s) 2073, 2617, 2983
TseFI GTSAC 2 cut(s) 456, 2297
TseI GCWGC 6 cut(s) 408, 851, 1367, 1370, 1792, 2363
Tsp45I GTSAC 2 cut(s) 456, 2297
TspGWI ACGGA 1 cut(s) 2396
TspRI CASTG 3 cut(s) 2073, 2617, 2983
Tth111I GACNNNGTC 1 cut(s) 278
VspI ATTAAT 1 cut(s) 3063
XagI CCTNNNNNAGG 1 cut(s) 627
XbaI TCTAGA 1 cut(s) 2447
XceI RCATGY 1 cut(s) 2363
XmiI GTMKAC 3 cut(s) 2172, 2763, 3150
XmnI GAANNNNTTC 5 cut(s) 1110, 1206, 1267, 2136, 3285
XspI CTAG 9 cut(s) 629, 645, 1364, 2433, 2448, 2675, 2807, 2823, 3090
Zsp2I ATGCAT 1 cut(s) 2650
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.