FvH4_6g07060

FES

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0007319
Physical Location & Seq
Forward (+)
4244012 .. 4252995
8984 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g07060.t1

Sequence Viewer

Length: 3822 bp
ATGAGAGTTTACGAAAGGAAGAACAGAGGGCACCGTGGGACTCAAGAGGGCGTTAGTGTAATGGAATCTATTGAGGTTCGAATCCCTGATACGCCCGTAAAGAAGACTCATAGACAGCTTTATGGGAATTCTATGCTCTCTGCCTCTACAAGTGCTTTAGAGGATGAAAACAACAAAGAGAAGGCAGAGTACTCGTCTGTGATGGATGTGGATAAGAAGGAAGAGGATTGTATTGGACTGACGAACACGAATATGGATTCAATTGGTCCCCTCGATTTGAACAAGGAGCCTGTTGAGGAGGAGTGGGACACTGAGGATCAGTTTGCCAATGGTTTTACGGCAGCATTGTTGCAAAGAGAAGATTCATCAAGGGAGGATGAGGAAGCGCTTGCTTTTAATCTTGTAAGTTCTCAGTCATCTTCCGACACCGTAATCCTTGAAAGTAGCAGTCGTTGTGGTTTTGGCAGCATTGAGGGTTCTTTCATGCCAGTACTTTCGCAACCTGAATCCATGTCACAATATAATTTGAAGACTCAGGAATTGGTTGACGGCATTTACAGTACAAATAATATTGATATCCTAAAAAGAAAACTACATGATGCTACACTTCAAAGTCCACGAAAGAGACTCAAGAGGCTGAAGAAAAAAGTTTACCGACCAAAAGTGGTACTAAATCTAGGAAGACCTGTACCTAGAATGGAACCGAAGACTCCCACCAAAAACAATAAAAGGTTGAAGCTCAAGACGCCAAAAAAGAAGAAAAATGTGTCATTGAATGAGAGTTCAAGTACAATCGATCTCAATCATGTCCCATTTACTTTAGATCCGGAGGAAACTGTATCAGAGAAAGAATCTTCAAGCATTGACATAGAAAAACACTCTGAAGATGGAACCCGTATTATATCAGAGAAGGCAGTGGAGTGTTGCATCACAAAATGCGTTGGTGATTCTCATATGGGAATATATTTTGTGAGGAAGAAAAAGAGGACGACAATGGGAAAGAAATGGGAACTGAAAGATTATAGTAGAATAATGGAACCAAATCTTCGACAGATACAAAAGAAAAAAAGGATGGGCAGAAGGAGAACGGAAGTAGAGAGGTGGCAGAAGATAGCTACCAAGTTTCAGGCAAACTATCTTAAACGGAGATATAATAAAAAGAGAAGAGCTGCCCTCGAAATCAGTCAAAAGGAATTGCGGAACATTTTTGACACCGAAAAGCAGTTGTCTCCATGTTTCATCACCCAATCTGGAGGTTTGAACATTGAAGAAAGCTTAACCGGAATAGTAAGCCTACTGGAAAGTGATAGTTTTGAGTTGTTCGTCGAATCAATAGATATATTTGAAGAGAATACAAGTCATGAAAGCAACCATGGGATACTAGTTTGTTACCAAAAAAATGAGAAACACGGTCATCATGTTGAGCTCGGTATCGTAGCTCATGGAAACGGTGAACTTGGTGTGGTAGCTACGACAAAGAGAAAAGGAAGGAGACAAGAAAAGCTTGAGAAATTAGAACACGGTGAACTTGCTCATCCATGGAAGCTTCAATTGCAAAATGGAGTTAAGGAGGGGGAAAAACACGATGAGGAGCATTCTGGATTTTGGGAGAAGGAGAGAGATTTTTTTCGTGGTCGGATTGACTTATTTCTGACTCGTTTGCGTATAGTTCAAGGAAATAGAACATTTTCAGAATGGGATGGATCAGTTATAGATTCGATAGTTGGAGTTTTTTTGACACAGAACGTATCAGATCATCTCTCCAGTTCTGCGTTTATTTCACTAGCTGCAGCTTTCCCATGTCAGCCAAGTTGTGAGCAATCAAAAATTGTTCAAATTGAGGAGTACGTAGAGGATGAGATTTCAAAGCTAAGCTGTGAGCCATCAAATGACCAAACATATGGTGAAAATATAAATACTACCAGCTCTACATCGGTTGGAAGTGACTTGGTATTTGATAAAGAGTTGTTCACAAAAGAAGCCACAACTACAATGGAGTGCGAAAATCAAAAGATAAGTGATTCAGAGATGGTGTTATCTTCTAGATGTGAAATAGGAGATTATGGAAGATCAAATCAAGAAGACATGCCATTTGATAAATCTGAGTGCTCATCAGGTGCTATTATAACTAACAACTCGCAGTCTTACTTCAATGAACTAAAACAAAAAGGAAGGAAGTTGAAGGCGGATGGACTTGATTGGGATTATTATAAGAGAGGAATAGAGAGTGAGCTTGGGAAAAAAAGACAGATTGAGCACATGGATTCAATAAACTGGCAGGCTGTTAGACAAGCAGATGTTGAAGACATTGCTGAAGCCATCAAGGGACGTGGACAACATCGTATCATCGCAGGACGAATAAAGAAATTCCTTAATGAAGTGAATCAAGATCATAAAGAAATAAACCTTGAGTGGCTAAGATGGGCTCCGGCAGAACTAGTAAGGACATACTTGACTAATATTGAAGGAATAGGCTTGAAAAGTGTTGAGTGTGTGCGCCTTTTAGCACTCAAGCAAATAGCGTTCCCGGTGGACGTTAATGTCGGCAGGATAGCTATTCGTTTAGGGTGGGTTCCTATGCAACCACTACCTGAAGATGTTCAGATACACGTATTAGAAGAGATGCCGGTTATGGACAATATTCAAAAGTACCTTTGGCCAAGGCTAACGAATTTGACGCACGAAGAATTGTATGAACTACATTATCATTTGATAACTTTTGGAAAGGTGTATTGTACCAAACGTAAGCCTAATTGTCGCTCATGTCCGATGAAGAACGAGTGCAAGTATTATCTCAGCGCATACGCCAGTGCTAAACATGCTGCATTACCAAAGTCAACGAAGAAAGAAAGACAAAAGAAAAGGGATGAACATCATGAAGTTGTTTGCAGTAATCATCTTTCTGATGGAGACGAGCCACTAAATCCAGCATCGCAGTCATATCTTGAGTATACTACCAAATTAGAATATAAAATGAACGGATGTGAACCGATAATTGAAGAACCAGCATCACCTGGAAGGTCATCATCAGAACCAGAATATGTGGAAAGAGACATTGAAGATTTATTCCTTGACAATCCATTAAATGAGTCTGTAACAAGAAGTCCATATATGAATGCTTTTCGAGGAACTGATATTTCATCACTAGTTCCCTTTCTGACCCATGAAGAAGCTTTCTCAATCCCTCCTCGCAGACTAAAGGAAGTAGAGCGTTTGAGGACAAGACATTCGGTATACGAGATCAAAGATGGAAGCACTTTGTTCAAAGAGCTTAAACTAGAGAGACTACACCCTAATGATGTTAGTCCTTATCTTTTTGCGATGTGCACACCAGTATCTCAACCACATGAAGACAGAAGCAGTTGCAAAGGGTCTAAAGATTCATCGTGTGACGGTGAGAAGACATGTTTTTCCTGTTGTAGTCTCTCAACACAAGATAATACTGATATGGTTCAAGGAACGATTTTGATACCTTGTAGAACAGCAACGAGGGGCAGTTTTCCTCTAAATGGTACATATTTCCAATTTAATGAGGTTTTCGCGGATCATGAATCCAGCATAAACCCCATTAAAGTTCAGAGAGATATGTTATGGAAACAAACAAGAAGAGATGTGTACTTTGGAACCTCAGCAAGTCCAATTCTCAAAGGTTTATCAACGTTTGAAGTTTATGAGTGTTTTGATAAAGGTTTTATTTGTGTGAGAGCATTCAATCGAAGAACACGTAGGCCTGAGCCTCTTCATGAAAGGTTTCATATTTCAACTGTCAAAACAACAAAGAAAGGGAAGAAGAAATCAGAAGAACAGGAGGAGGATGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Perm-CXXC RRM_DME

Protein Analysis

1274

Amino Acids

145.93

Weight (kDa)

6.51

Isoelectric Point (pI)

55.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_DME PF15628 1153 - 1253 3.9e-46 RRM in Demeter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 2126, 2211
AasI GACNNNNNNGTC 1 cut(s) 2540
AccB1I GGYRCC 1 cut(s) 30
AccI GTMKAC 2 cut(s) 2951, 3234
AccII CGCG 1 cut(s) 3542
AccIII TCCGGA 1 cut(s) 826
AciI CCGC 3 cut(s) 1198, 2186, 3542
AclI AACGTT 1 cut(s) 3659
AclWI GGATC 4 cut(s) 324, 818, 1711, 3552
AcoI YGGCCR 1 cut(s) 2657
AcsI RAATTY 3 cut(s) 127, 2366, 2671
AcuI CTGAAG 4 cut(s) 659, 903, 2334, 2613
AcyI GRCGYC 1 cut(s) 746
AfeI AGCGCT 1 cut(s) 387
AfiI CCNNNNNNNGG 1 cut(s) 3509
AflIII ACRYGT 3 cut(s) 2608, 3404, 3722
AhlI ACTAGT 3 cut(s) 1381, 2437, 3145
AjiI CACGTC 1 cut(s) 2330
AjnI CCWGG 1 cut(s) 3013
Alw21I GWGCWC 4 cut(s) 1428, 2111, 2259, 3329
Alw26I GTCTC 7 cut(s) 619, 1233, 1486, 2904, 3045, 3277, 3428
Alw44I GTGCAC 1 cut(s) 3325
AlwI GGATC 4 cut(s) 324, 818, 1711, 3552
Aor13HI TCCGGA 1 cut(s) 826
Aor51HI AGCGCT 1 cut(s) 387
AoxI GGCC 2 cut(s) 2657, 3728
ApaLI GTGCAC 1 cut(s) 3325
ApeKI GCWGC 6 cut(s) 341, 465, 1169, 1787, 1790, 2822
ApoI RAATTY 3 cut(s) 127, 2366, 2671
ArsI GACNNNNNNTTYG 6 cut(s) 1937, 1969, 2075, 2107, 3211, 3243
Asp700I GAANNNNTTC 3 cut(s) 1687, 2598, 3750
AspLEI GCGC 3 cut(s) 388, 2499, 2801
AspS9I GGNCC 1 cut(s) 266
AsuC2I CCSGG 1 cut(s) 2528
AsuHPI GGTGA 7 cut(s) 956, 1234, 1463, 1535, 1916, 3003, 3407
AsuII TTCGAA 1 cut(s) 79
AvaII GGWCC 1 cut(s) 266
BaeGI GKGCMC 2 cut(s) 33, 3329
BaeI ACNNNNGTAYC 4 cut(s) 81, 114, 3318, 3351
BalI TGGCCA 1 cut(s) 2659
BanI GGYRCC 1 cut(s) 30
BanII GRGCYC 2 cut(s) 1428, 2428
BarI GAAGNNNNNNTAC 2 cut(s) 173, 205
BbsI GAAGAC 8 cut(s) 110, 536, 688, 713, 2088, 2310, 3357, 3407
Bbv12I GWGCWC 4 cut(s) 1428, 2111, 2259, 3329
BbvCI CCTCAGC 1 cut(s) 3628
BbvI GCAGC 6 cut(s) 353, 477, 1156, 1774, 1802, 2809
BceAI ACGGC 2 cut(s) 354, 565
BcgI CGANNNNNNTGC 2 cut(s) 174, 208
BciT130I CCWGG 1 cut(s) 3015
BciVI GTATCC 1 cut(s) 1371
BcnI CCSGG 1 cut(s) 2528
BcoDI GTCTC 7 cut(s) 619, 1233, 1486, 2904, 3045, 3277, 3428
BcuI ACTAGT 3 cut(s) 1381, 2437, 3145
BfaI CTAG 8 cut(s) 677, 693, 1382, 1784, 2043, 2438, 3146, 3278
BfmI CTRYAG 1 cut(s) 1788
BfoI RGCGCY 1 cut(s) 389
BfuI GTATCC 1 cut(s) 1371
BisI GCNGC 6 cut(s) 342, 466, 1170, 1788, 1791, 2823
BlpI GCTNAGC 1 cut(s) 1871
BlsI GCNGC 6 cut(s) 343, 467, 1171, 1789, 1792, 2824
BmcAI AGTACT 2 cut(s) 191, 492
Bme1390I CCNGG 2 cut(s) 2528, 3015
Bme18I GGWCC 1 cut(s) 266
BmgBI CACGTC 1 cut(s) 2330
BmgT120I GGNCC 1 cut(s) 266
BmiI GGNNCC 9 cut(s) 32, 268, 288, 702, 892, 1038, 2427, 2574, 3625
BmrFI CCNGG 2 cut(s) 2528, 3015
BmsI GCATC 5 cut(s) 589, 936, 2613, 2939, 3017
BpiI GAAGAC 8 cut(s) 110, 536, 688, 713, 2088, 2310, 3357, 3407
BplI GAGNNNNNCTC 2 cut(s) 1158, 1190
BpmI CTGGAG 2 cut(s) 1272, 1747
Bpu10I CCTNAGC 2 cut(s) 3628, 3732
Bpu1102I GCTNAGC 1 cut(s) 1871
Bpu14I TTCGAA 1 cut(s) 79
BpuEI CTTGAG 7 cut(s) 27, 614, 725, 1526, 2429, 2495, 2966
BpuMI CCSGG 1 cut(s) 2528
Bsa29I ATCGAT 1 cut(s) 795
BsaAI YACGTR 3 cut(s) 1849, 2611, 3725
BsaBI GATNNNNATC 2 cut(s) 801, 2871
BsaHI GRCGYC 1 cut(s) 746
BsaJI CCNNGG 4 cut(s) 34, 1372, 1538, 2660
BsaWI WCCGGW 2 cut(s) 826, 1280
Bsc4I CCNNNNNNNGG 1 cut(s) 3509
Bse118I RCCGGY 1 cut(s) 2626
Bse1I ACTGG 6 cut(s) 488, 1302, 1764, 2279, 2808, 3332
Bse3DI GCAATG 1 cut(s) 2307
Bse8I GATNNNNATC 2 cut(s) 801, 2871
BseAI TCCGGA 1 cut(s) 826
BseBI CCWGG 1 cut(s) 3015
BseCI ATCGAT 1 cut(s) 795
BseDI CCNNGG 4 cut(s) 34, 1372, 1538, 2660
BseJI GATNNNNATC 2 cut(s) 801, 2871
BseLI CCNNNNNNNGG 1 cut(s) 3509
BseMI GCAATG 1 cut(s) 2307
BseMII CTCAG 7 cut(s) 303, 425, 548, 2094, 2809, 3642, 3723
BseNI ACTGG 6 cut(s) 488, 1302, 1764, 2279, 2808, 3332
BseRI GAGGAG 5 cut(s) 311, 314, 1604, 1856, 3177
BseSI GKGCMC 2 cut(s) 33, 3329
BseXI GCAGC 6 cut(s) 353, 477, 1156, 1774, 1802, 2809
Bsh1236I CGCG 1 cut(s) 3542
BshFI GGCC 2 cut(s) 2659, 3730
BshNI GGYRCC 1 cut(s) 30
BshVI ATCGAT 1 cut(s) 795
BsiHKAI GWGCWC 4 cut(s) 1428, 2111, 2259, 3329
BsiSI CCGG 5 cut(s) 827, 1281, 2429, 2528, 2627
BslFI GGGAC 5 cut(s) 52, 252, 320, 794, 2340
BslI CCNNNNNNNGG 1 cut(s) 3509
BsmAI GTCTC 7 cut(s) 619, 1233, 1486, 2904, 3045, 3277, 3428
BsmBI CGTCTC 1 cut(s) 2904
BsmFI GGGAC 5 cut(s) 52, 252, 320, 794, 2340
BsmI GAATGC 3 cut(s) 1594, 3121, 3707
BsnI GGCC 2 cut(s) 2659, 3730
Bsp119I TTCGAA 1 cut(s) 79
Bsp1286I GDGCHC 6 cut(s) 33, 1428, 2111, 2259, 2428, 3329
Bsp13I TCCGGA 1 cut(s) 826
Bsp143I GATC 9 cut(s) 316, 796, 823, 1703, 1753, 2069, 2389, 3240, 3544
Bsp1720I GCTNAGC 1 cut(s) 1871
Bsp19I CCATGG 2 cut(s) 1372, 1538
BspACI CCGC 3 cut(s) 1198, 2186, 3542
BspANI GGCC 2 cut(s) 2659, 3730
BspCNI CTCAG 7 cut(s) 304, 424, 547, 2095, 2808, 3641, 3724
BspDI ATCGAT 1 cut(s) 795
BspEI TCCGGA 1 cut(s) 826
BspFNI CGCG 1 cut(s) 3542
BspHI TCATGA 4 cut(s) 1360, 2875, 3547, 3742
BspLI GGNNCC 9 cut(s) 32, 268, 288, 702, 892, 1038, 2427, 2574, 3625
BspMAI CTGCAG 1 cut(s) 1792
BspPI GGATC 4 cut(s) 324, 818, 1711, 3552
BspQI GCTCTTC 1 cut(s) 1159
BspT104I TTCGAA 1 cut(s) 79
BspT107I GGYRCC 1 cut(s) 30
BsrDI GCAATG 1 cut(s) 2307
BsrFI RCCGGY 1 cut(s) 2626
BsrI ACTGG 6 cut(s) 488, 1302, 1764, 2279, 2808, 3332
BssAI RCCGGY 1 cut(s) 2626
BssECI CCNNGG 4 cut(s) 34, 1372, 1538, 2660
BssMI GATC 9 cut(s) 316, 796, 823, 1703, 1753, 2069, 2389, 3240, 3544
BssNAI GTATAC 2 cut(s) 2952, 3235
BssNI GRCGYC 1 cut(s) 746
BssT1I CCWWGG 3 cut(s) 1372, 1538, 2660
Bst1107I GTATAC 2 cut(s) 2952, 3235
Bst2UI CCWGG 1 cut(s) 3015
Bst4CI ACNGT 9 cut(s) 35, 430, 560, 838, 1412, 1451, 1523, 3395, 3766
Bst6I CTCTTC 6 cut(s) 216, 1159, 1341, 2613, 3601, 3744
BstACI GRCGYC 1 cut(s) 746
BstBAI YACGTR 3 cut(s) 1849, 2611, 3725
BstBI TTCGAA 1 cut(s) 79
BstC8I GCNNGC 2 cut(s) 390, 2280
BstDEI CTNAG 9 cut(s) 312, 411, 534, 1871, 2103, 2417, 2795, 3628, 3732
BstDSI CCRYGG 3 cut(s) 34, 1372, 1538
BstFNI CGCG 1 cut(s) 3542
BstH2I RGCGCY 1 cut(s) 389
BstHHI GCGC 3 cut(s) 388, 2499, 2801
BstKTI GATC 9 cut(s) 319, 799, 826, 1706, 1756, 2072, 2392, 3243, 3547
BstMAI GTCTC 7 cut(s) 619, 1233, 1486, 2904, 3045, 3277, 3428
BstMBI GATC 9 cut(s) 316, 796, 823, 1703, 1753, 2069, 2389, 3240, 3544
BstMWI GCNNNNNNNGC 3 cut(s) 745, 1552, 2819
BstNI CCWGG 1 cut(s) 3015
BstNSI RCATGY 3 cut(s) 2089, 2822, 3408
BstSCI CCNGG 2 cut(s) 2526, 3013
BstSFI CTRYAG 1 cut(s) 1788
BstSLI GKGCMC 2 cut(s) 33, 3329
BstSNI TACGTA 1 cut(s) 1849
BstUI CGCG 1 cut(s) 3542
BstV1I GCAGC 6 cut(s) 353, 477, 1156, 1774, 1802, 2809
BstV2I GAAGAC 8 cut(s) 110, 536, 688, 713, 2088, 2310, 3357, 3407
BstX2I RGATCY 1 cut(s) 823
BstXI CCANNNNNNTGG 1 cut(s) 1901
BstYI RGATCY 1 cut(s) 823
BstZ17I GTATAC 2 cut(s) 2952, 3235
Bsu15I ATCGAT 1 cut(s) 795
BsuI GTATCC 1 cut(s) 1371
BsuRI GGCC 2 cut(s) 2659, 3730
BsuTUI ATCGAT 1 cut(s) 795
BtgI CCRYGG 3 cut(s) 34, 1372, 1538
BtgZI GCGATG 3 cut(s) 2332, 2916, 3335
BtrI CACGTC 1 cut(s) 2330
BtsI GCAGTG 1 cut(s) 921
BtsIMutI CAGTG 3 cut(s) 309, 921, 2815
Cac8I GCNNGC 2 cut(s) 390, 2280
CciI TCATGA 4 cut(s) 1360, 2875, 3547, 3742
CfoI GCGC 3 cut(s) 388, 2499, 2801
Cfr10I RCCGGY 1 cut(s) 2626
Cfr13I GGNCC 1 cut(s) 266
ClaI ATCGAT 1 cut(s) 795
CseI GACGC 2 cut(s) 754, 2686
CspCI CAANNNNNGTGG 2 cut(s) 2311, 2346
DdeI CTNAG 9 cut(s) 312, 411, 534, 1871, 2103, 2417, 2795, 3628, 3732
DpnI GATC 9 cut(s) 318, 798, 825, 1705, 1755, 2071, 2391, 3242, 3546
DpnII GATC 9 cut(s) 316, 796, 823, 1703, 1753, 2069, 2389, 3240, 3544
DrdI GACNNNNNNGTC 1 cut(s) 2540
DseDI GACNNNNNNGTC 1 cut(s) 2540
EaeI YGGCCR 1 cut(s) 2657
Eam1104I CTCTTC 6 cut(s) 216, 1159, 1341, 2613, 3601, 3744
EarI CTCTTC 6 cut(s) 216, 1159, 1341, 2613, 3601, 3744
EciI GGCGGA 1 cut(s) 2201
Ecl136II GAGCTC 1 cut(s) 1426
Eco105I TACGTA 1 cut(s) 1849
Eco130I CCWWGG 3 cut(s) 1372, 1538, 2660
Eco147I AGGCCT 1 cut(s) 3730
Eco24I GRGCYC 2 cut(s) 1428, 2428
Eco32I GATATC 1 cut(s) 577
Eco47I GGWCC 1 cut(s) 266
Eco47III AGCGCT 1 cut(s) 387
Eco53kI GAGCTC 1 cut(s) 1426
Eco57I CTGAAG 4 cut(s) 659, 903, 2334, 2613
EcoICRI GAGCTC 1 cut(s) 1426
EcoRI GAATTC 1 cut(s) 127
EcoRII CCWGG 1 cut(s) 3013
EcoRV GATATC 1 cut(s) 577
EcoT14I CCWWGG 3 cut(s) 1372, 1538, 2660
EcoT38I GRGCYC 2 cut(s) 1428, 2428
ErhI CCWWGG 3 cut(s) 1372, 1538, 2660
Esp3I CGTCTC 1 cut(s) 2904
FalI AAGNNNNNCTT 2 cut(s) 1488, 1520
FaqI GGGAC 5 cut(s) 52, 252, 320, 794, 2340
FauNDI CATATG 2 cut(s) 954, 1900
FblI GTMKAC 2 cut(s) 2951, 3234
Fnu4HI GCNGC 6 cut(s) 342, 466, 1170, 1788, 1791, 2823
FriOI GRGCYC 2 cut(s) 1428, 2428
Fsp4HI GCNGC 6 cut(s) 342, 466, 1170, 1788, 1791, 2823
FspBI CTAG 8 cut(s) 677, 693, 1382, 1784, 2043, 2438, 3146, 3278
GlaI GCGC 3 cut(s) 387, 2498, 2800
GluI GCNGC 6 cut(s) 342, 466, 1170, 1788, 1791, 2823
GsuI CTGGAG 2 cut(s) 1272, 1747
HaeII RGCGCY 1 cut(s) 389
HaeIII GGCC 2 cut(s) 2659, 3730
HapII CCGG 5 cut(s) 827, 1281, 2429, 2528, 2627
HgaI GACGC 2 cut(s) 754, 2686
HhaI GCGC 3 cut(s) 388, 2499, 2801
Hin1I GRCGYC 1 cut(s) 746
Hin6I GCGC 3 cut(s) 386, 2497, 2799
HinP1I GCGC 3 cut(s) 386, 2497, 2799
HincII GTYRAC 2 cut(s) 547, 2838
HindII GTYRAC 2 cut(s) 547, 2838
HindIII AAGCTT 4 cut(s) 1273, 1502, 1544, 3171
HpaII CCGG 5 cut(s) 827, 1281, 2429, 2528, 2627
HphI GGTGA 7 cut(s) 956, 1234, 1463, 1535, 1916, 3003, 3407
Hpy99I CGWCG 1 cut(s) 1328
HpyCH4III ACNGT 9 cut(s) 35, 430, 560, 838, 1412, 1451, 1523, 3395, 3766
HpyCH4IV ACGT 8 cut(s) 1746, 1848, 2329, 2535, 2610, 2743, 3659, 3724
HpyF10VI GCNNNNNNNGC 3 cut(s) 745, 1552, 2819
HpyF3I CTNAG 9 cut(s) 312, 411, 534, 1871, 2103, 2417, 2795, 3628, 3732
HpySE526I ACGT 8 cut(s) 1746, 1848, 2329, 2535, 2610, 2743, 3659, 3724
Hsp92I GRCGYC 1 cut(s) 746
HspAI GCGC 3 cut(s) 386, 2497, 2799
Kpn2I TCCGGA 1 cut(s) 826
Kzo9I GATC 9 cut(s) 316, 796, 823, 1703, 1753, 2069, 2389, 3240, 3544
LguI GCTCTTC 1 cut(s) 1159
LmnI GCTCC 3 cut(s) 286, 1591, 2431
Lsp1109I GCAGC 6 cut(s) 353, 477, 1156, 1774, 1802, 2809
LweI GCATC 5 cut(s) 589, 936, 2613, 2939, 3017
MaeI CTAG 8 cut(s) 677, 693, 1382, 1784, 2043, 2438, 3146, 3278
MaeII ACGT 8 cut(s) 1746, 1848, 2329, 2535, 2610, 2743, 3659, 3724
MaeIII GTNAC 5 cut(s) 513, 1388, 1943, 3094, 3389
MalI GATC 9 cut(s) 318, 798, 825, 1705, 1755, 2071, 2391, 3242, 3546
MboI GATC 9 cut(s) 316, 796, 823, 1703, 1753, 2069, 2389, 3240, 3544
MfeI CAATTG 2 cut(s) 261, 1550
MflI RGATCY 1 cut(s) 823
MhlI GDGCHC 6 cut(s) 33, 1428, 2111, 2259, 2428, 3329
MlsI TGGCCA 1 cut(s) 2659
MluNI TGGCCA 1 cut(s) 2659
MlyI GAGTC 7 cut(s) 34, 100, 526, 621, 703, 1648, 3098
MmeI TCCRAC 4 cut(s) 447, 1616, 1705, 1918
Mox20I TGGCCA 1 cut(s) 2659
MroI TCCGGA 1 cut(s) 826
MroXI GAANNNNTTC 3 cut(s) 1687, 2598, 3750
MscI TGGCCA 1 cut(s) 2659
MslI CAYNNNNRTG 2 cut(s) 251, 3294
Msp20I TGGCCA 1 cut(s) 2659
MspI CCGG 5 cut(s) 827, 1281, 2429, 2528, 2627
MspR9I CCNGG 2 cut(s) 2528, 3015
MunI CAATTG 2 cut(s) 261, 1550
Mva1269I GAATGC 3 cut(s) 1594, 3121, 3707
MvaI CCWGG 1 cut(s) 3015
MvnI CGCG 1 cut(s) 3542
MwoI GCNNNNNNNGC 3 cut(s) 745, 1552, 2819
NciI CCSGG 1 cut(s) 2528
NcoI CCATGG 2 cut(s) 1372, 1538
NdeI CATATG 2 cut(s) 954, 1900
NdeII GATC 9 cut(s) 316, 796, 823, 1703, 1753, 2069, 2389, 3240, 3544
NlaIV GGNNCC 9 cut(s) 32, 268, 288, 702, 892, 1038, 2427, 2574, 3625
NmuCI GTSAC 3 cut(s) 513, 1943, 3389
NspI RCATGY 3 cut(s) 2089, 2822, 3408
NspV TTCGAA 1 cut(s) 79
PagI TCATGA 4 cut(s) 1360, 2875, 3547, 3742
PceI AGGCCT 1 cut(s) 3730
PciI ACATGT 1 cut(s) 3404
PciSI GCTCTTC 1 cut(s) 1159
PcsI WCGNNNNNNNCGW 1 cut(s) 3721
PctI GAATGC 3 cut(s) 1594, 3121, 3707
PdmI GAANNNNTTC 3 cut(s) 1687, 2598, 3750
PkrI GCNGC 6 cut(s) 343, 467, 1171, 1789, 1792, 2824
PleI GAGTC 7 cut(s) 34, 100, 526, 621, 703, 1648, 3097
PpsI GAGTC 7 cut(s) 34, 100, 526, 621, 703, 1648, 3097
Ppu21I YACGTR 3 cut(s) 1849, 2611, 3725
PscI ACATGT 1 cut(s) 3404
PsiI TTATAA 2 cut(s) 2126, 2211
Psp124BI GAGCTC 1 cut(s) 1428
Psp1406I AACGTT 1 cut(s) 3659
Psp6I CCWGG 1 cut(s) 3013
PspGI CCWGG 1 cut(s) 3013
PspN4I GGNNCC 9 cut(s) 32, 268, 288, 702, 892, 1038, 2427, 2574, 3625
PspPI GGNCC 1 cut(s) 266
PstI CTGCAG 1 cut(s) 1792
PsuI RGATCY 1 cut(s) 823
RseI CAYNNNNRTG 2 cut(s) 251, 3294
SacI GAGCTC 1 cut(s) 1428
SapI GCTCTTC 1 cut(s) 1159
SatI GCNGC 6 cut(s) 342, 466, 1170, 1788, 1791, 2823
Sau3AI GATC 9 cut(s) 316, 796, 823, 1703, 1753, 2069, 2389, 3240, 3544
Sau96I GGNCC 1 cut(s) 266
ScaI AGTACT 2 cut(s) 191, 492
SchI GAGTC 7 cut(s) 34, 100, 526, 621, 703, 1648, 3098
ScrFI CCNGG 2 cut(s) 2528, 3015
SduI GDGCHC 6 cut(s) 33, 1428, 2111, 2259, 2428, 3329
SfaNI GCATC 5 cut(s) 589, 936, 2613, 2939, 3017
SfcI CTRYAG 1 cut(s) 1788
SfuI TTCGAA 1 cut(s) 79
SinI GGWCC 1 cut(s) 266
SmiMI CAYNNNNRTG 2 cut(s) 251, 3294
SmlI CTYRAG 7 cut(s) 42, 629, 740, 1505, 2408, 2510, 2945
SmoI CTYRAG 7 cut(s) 42, 629, 740, 1505, 2408, 2510, 2945
SnaBI TACGTA 1 cut(s) 1849
SpeI ACTAGT 3 cut(s) 1381, 2437, 3145
SseBI AGGCCT 1 cut(s) 3730
SsiI CCGC 3 cut(s) 1198, 2186, 3542
SspI AATATT 3 cut(s) 571, 2461, 2641
SspMI CTAG 8 cut(s) 677, 693, 1382, 1784, 2043, 2438, 3146, 3278
SstI GAGCTC 1 cut(s) 1428
StuI AGGCCT 1 cut(s) 3730
StyD4I CCNGG 2 cut(s) 2526, 3013
StyI CCWWGG 3 cut(s) 1372, 1538, 2660
TaaI ACNGT 9 cut(s) 35, 430, 560, 838, 1412, 1451, 1523, 3395, 3766
TaiI ACGT 8 cut(s) 1749, 1851, 2332, 2538, 2613, 2746, 3662, 3727
TaqI TCGA 9 cut(s) 79, 273, 795, 1048, 1176, 1326, 1718, 3124, 3715
TatI WGTACW 5 cut(s) 189, 490, 560, 788, 3615
TscAI CASTG 3 cut(s) 316, 921, 2815
TseFI GTSAC 3 cut(s) 513, 1943, 3389
TseI GCWGC 6 cut(s) 341, 465, 1169, 1787, 1790, 2822
Tsp45I GTSAC 3 cut(s) 513, 1943, 3389
TspGWI ACGGA 3 cut(s) 1103, 1159, 2994
TspRI CASTG 3 cut(s) 316, 921, 2815
VneI GTGCAC 1 cut(s) 3325
VpaK11BI GGWCC 1 cut(s) 266
XapI RAATTY 3 cut(s) 127, 2366, 2671
XbaI TCTAGA 1 cut(s) 2042
XceI RCATGY 3 cut(s) 2089, 2822, 3408
XcmI CCANNNNNNNNNTGG 1 cut(s) 1990
XmiI GTMKAC 2 cut(s) 2951, 3234
XmnI GAANNNNTTC 3 cut(s) 1687, 2598, 3750
XspI CTAG 8 cut(s) 677, 693, 1382, 1784, 2043, 2438, 3146, 3278
ZrmI AGTACT 2 cut(s) 191, 492
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.