Rh3DG078600

FES

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0007319
Physical Location & Seq
Forward (+)
6105830 .. 6116318
10489 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG078600.1

Sequence Viewer

Length: 3966 bp
ATGGCTTTCCAACTCTTCGAAACAAACCACAAATCAACAGTAGTACAGAACATGATGGCACCGATGTCAAAAGTTTACGAGAGGCAGAAGAGACAGCACGTTGGGTCTGAAGACAACAGTGTCATGGAACGGGTTCAGGTTCAGATCCCAGATACTCCTGCAAAGAAAACTCAGAGACAACGTTCTAGCGGGATTTCTATGCTTACTGCCCCTACAAATTCTTTAGAGGAAAACAAAGAGAACCCGATTGCAGAGGACTTGTCTGCAATGGACGACGAGGTAGTGGATTGCAGTGGACTTATGGACACGATTTTGGATTCCATTGGTTCCTTGGATTTGAACAAGCCTGTGTGGGAGGAGTGGGAGACTGAGGAATTTGCTTATGGTTATGGGGCCGCATTATTGCAAACAGAAGATCTTGAAAGGGAGGATGAGGAAGTGTTGACATATAATCTTCCATGTTCTCAGTCATCTTCCAATACCGAAAATCCTGAAAATCACAGTTGCGGTTTTGGCAGCATAGAGGGTTCTTTCATGCCAGTTGTGCAACCTGGTTATGCTGCTGAATCCATGTCACAATATAATTTGAAGAATCAGGAATTAGTTGATGGCAAGTACAATATTGCTGATACAAATTATATTGGTAGCTTAAAAAGAAAACTACATGCTGCTACACTTCAAATGCCACGGAAGAGACTCAAGAGGCCGAAGAAAAAAGTTTACCGGCCAAAAGTGGTACGTAATTTAGGAAGGCCTATACCTAGAATGGAACCCAACACTCCCACCAAAAAGAATGAAAGGATGAAGCTCAAAACACCAAAAATGAAGAAAAACGTGTCATTGAAGAAGTCTTCGAGCACAATCAACCTCAACCATGTCCGAGTTCCTTTAGATTTGGAGCCTATTGTATCAGAGGGAGAATTTTCAAGCATTGACATAGAAAAGCACTCTAACGATGGAACCCGCATCATATCAGAGAAGACAGTGGAGCATTGCATCACAAAATGCATTGATTTAGGATTAAATTTTTTGACGAAGAAAAAGAGGACGACGATGGGAAAGAAATGGGAAGTGAAAGACTATAGTAGAATAATGGAGCCGAATCTTCTGAAAATAAAAAAGAAAAAAAGGATGGACAGGAGGAGATGGGAAGTAGACGTGTGGAAAAAAATAGCTAGCAAGTTTCAGACAAACTATCTTAAACGGAGACGCAATAAAAAGAGAAGAGCTGCCCCCGAAATCAGTCAACAGGAAGTGCGCAACATCTTTAACACCGAAAAGCAGTTGTCTCCATGCTTCATCACCGAATCAGGAGTATTGAACATTGAAGAAAGCTTAACAGGAATGGTGAGCTTACTGGAAAGTGATAGTTTTGAGTTGTTCTTCAAATCAACAGATATACCTGAAGATAATATCAATGTTGTTGTGCAAAAACATCAACTTAACCATGGGATAGTTGTTTGTTACCAAGAAAATGAGAAACATGGTCCTCACATTGAGCTGGGTATCGTAGCCAGTGCAAATGGTGAGCTTGGTATGGTAGCCACGACAAAGGGAAAGGGAAGGAGGCAAGAAAAGCTTGAGAAATTAGAACATGGTGAACTAGCTCACCCATGGAAGCTTCAATTGCAAAATGAAGTTAAGGAGGGGGACAAACATGACCCGAACTCTGAATTTTGGGATAAGGAGAGACAATTCTTTCAGGGTCGGATCAACTTATTTTTAACTCGTATGCGCATAGTTCAAGGAAATAGAAAATTTTCAGAATGGGATGGATCAGTTATGGATTCGATAGTCGGAGTTTTTTTAACACAGAACGTATCAGATCATCTATCCAGTTCTGCCTTTATATCACTCGCTGCAGCTTTCCCATATCAGCCCAGTTGTAACCAAGAATTAACTTATGCAAACTCAGAGCCAAGTTGTGAGCAATCAAGAATTGTTAAAATTGAGGAATTAGTAGAGGGTGGAATCTCAAAGTCCAGCTGTGAACAATCAAATGACCAAACATATTGTGAAGATATAAATAGTGGCAGCCATAAATTGGTTGGAAGTAACATGGTATTTGATAAAGAGTCGTTCACACAAGAAGCCACAACTACAATGAAGTACGAAAATCAGAAGTCAAGTGATTGCGAAATGGTATTAGCCAAACAAGTTGAGTTGCAGCAAAATTTTGAGGGTCAAGATTATTACGTGAACATGCCATCTGATAAGCCTGACTGCTCGATTGATATTATTACTGAAAATTCGCTTTCTTACTTCAATGAGGAAAAGCAAAGAGGAAAGAAGTTGAAGGTGGATGCTCGAGAAATAAATAATGAGGTTGCTATGGTGAATGGAAAACCTGATTGGGATTATTATAGGAGAGAAATTGTAAAGGAAAGAAAGATCAGGCACATGGCGGATTCGGTAGACTGGCAGGCCGTTAGACAAGCAGATGTTGGTGATGTTGCTGAAGCCATCAAAGGACGTGGACAACATCGTGTCATCGCAGCTCGAATAAAGAAATTTCTTAATGACGTGAATCAAGACCATAAAGAAATAAACCTTGAGTGGCTAAGATGGGCTCCAGACAAACTAGCAAGGAAATATTTGACTAATATTGAGGGAATTGGCCTGAAAAGTGTGGAGTGCGTGCGCCTTTTAGCACTCAAGCAGATAGCCTTCCCGGTGGACGTTAATGTTGGCAGGATAGCCATTCGTTTAGGGTGGGTGCCTATGCAACCATTACCTGAAGATGTTCAGATACACGTATTAGAAGAGATGCCGGTTATGGACAATATTCAAAAGTATCTTTGGCCTCGGCTAATGAAATTGAAGTTCGAAGAATTGTATGAACTACACTATCAAATGATAACCTTTGGAAAGGTTTATTGTACCAAACGTAAACCTAATTGTCATTCATGTCCAATGAAGAACGAGTGCAAGTATTATCTTAGTGCATATGCAAGTTCAAAATATGCTGCTTTACCGAAGTCAATGAAGAAGCAAGGACAAAAGAAAACTGATGTGGTTATTTGCAATAATCATCTTTCTTCTGGGTATGAGTCAGTAAATGCAGCATCACAATCATATCTTGAGGACACTACTAAATTAGAATATCAAATAAAAGATTGTGAACCGATAATTGAAGAACCAGCATCACCTCGACCGTCATTAGCGGAACCAGAATATGTGGAAAGAGACATTGAAGATTTATTCCTTGATGATCCTTTTAAAGAGTTCGTAACAAGAAGTCCATATATGAATACATTTCGAGGAAGTGATATCTCAACACTAGTGCCCTTTATGACCCCAGAAGAAGCTGCTTTCATCCCCGCTCGTAGACTAAAAGAAGTGGACCGTTTGAGGACAAAACACTCAGTCTACGTGATTAAAGATCAAGGCCCTTTATTTAAAGAGCTACAACTAGAGAGACGTCACCCTCATGATAATAGCCCTTACCTTTTTGCCTTGTGCACATCAGATCAAATGGCAAAAGTATCTCAATCACCTGATCAAAACAGATGCAGTTGCAAGGGATCTAATGATTCATCATGTGATGGTGAGAAGACATGTTTTTCTTGTAACAGTCTTTCAACACAAGCTAATACTGTTCAAGGAACAATTTTGATACCTTGTAGAACAGCAACGAGGGGAAGTTTTCCATTAAACGGTACATATTTTCAAATTAATGAGGTTTTTGCGGATCATGAATCCAGTATACGCCCCATCAGCGTTCATAGAGATTTGTTATGGGATCAGACGAGAAGAGATGTATACTTTGGAACATCAACAAGTGCAATTCTTAAAGGCTTATCAACATATGACGTTGCGATGTGTTTTAAGGAAGGTTCTATTTGCGTTAGAGCATTTAATCGGGAAACACGCAGGCCTGAGCCTCTATTAGAAAGGTTTCACATTTCAACCGTCAAAACCGCAAAGAAAGGAAAGAAAAAAGCGGAACAGAAAGAAGACGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Perm-CXXC RRM_DME

Protein Analysis

1321

Amino Acids

151.42

Weight (kDa)

7.92

Isoelectric Point (pI)

48.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_DME PF15628 1202 - 1302 1.6e-45 RRM in Demeter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 119
AatII GACGTC 1 cut(s) 3424
Acc16I TGCGCA 2 cut(s) 1259, 1736
AccB1I GGYRCC 2 cut(s) 58, 2716
AccB7I CCANNNNNTGG 1 cut(s) 2044
AccBSI CCGCTC 1 cut(s) 3323
AccI GTMKAC 6 cut(s) 1155, 2415, 3328, 3369, 3706, 3762
AclI AACGTT 1 cut(s) 181
AclWI GGATC 7 cut(s) 139, 1718, 1783, 3206, 3532, 3699, 3750
AcoI YGGCCR 1 cut(s) 725
AcsI RAATTY 9 cut(s) 217, 374, 920, 1024, 1673, 1757, 2173, 2248, 2510
AcuI CTGAAG 4 cut(s) 129, 1425, 2478, 2757
AcyI GRCGYC 1 cut(s) 3421
AfaI GTAC 6 cut(s) 45, 617, 738, 2111, 2881, 3661
AfiI CCNNNNNNNGG 2 cut(s) 2044, 3656
AflIII ACRYGT 4 cut(s) 834, 1158, 2752, 3557
AhlI ACTAGT 1 cut(s) 3280
AjiI CACGTC 3 cut(s) 1159, 2474, 2524
AjnI CCWGG 1 cut(s) 550
AjuI GAANNNNNNNTTGG 2 cut(s) 2335, 2367
Alw21I GWGCWC 2 cut(s) 860, 3464
Alw26I GTCTC 9 cut(s) 85, 169, 359, 688, 1201, 1293, 1684, 3180, 3412
Alw44I GTGCAC 1 cut(s) 3460
AlwI GGATC 7 cut(s) 139, 1718, 1783, 3206, 3532, 3699, 3750
Ama87I CYCGRG 1 cut(s) 2307
AoxI GGCC 9 cut(s) 393, 704, 725, 752, 2424, 2617, 2801, 3388, 3875
ApaLI GTGCAC 1 cut(s) 3460
ApoI RAATTY 9 cut(s) 217, 374, 920, 1024, 1673, 1757, 2173, 2248, 2510
AseI ATTAAT 1 cut(s) 3675
Asp700I GAANNNNTTC 4 cut(s) 132, 1759, 2742, 3897
AspLEI GCGC 3 cut(s) 1260, 1737, 2643
AspS9I GGNCC 4 cut(s) 393, 1487, 3343, 3389
AsuC2I CCSGG 1 cut(s) 2672
AsuII TTCGAA 2 cut(s) 18, 2826
AsuNHI GCTAGC 1 cut(s) 1175
AvaI CYCGRG 1 cut(s) 2307
AvaII GGWCC 2 cut(s) 1487, 3343
BaeGI GKGCMC 2 cut(s) 3288, 3464
BanI GGYRCC 2 cut(s) 58, 2716
BanII GRGCYC 1 cut(s) 2572
BbsI GAAGAC 4 cut(s) 117, 843, 986, 3560
Bbv12I GWGCWC 2 cut(s) 860, 3464
BceAI ACGGC 1 cut(s) 2411
BciT130I CCWGG 1 cut(s) 552
BclI TGATCA 1 cut(s) 3499
BcnI CCSGG 1 cut(s) 2672
BcoDI GTCTC 9 cut(s) 85, 169, 359, 688, 1201, 1293, 1684, 3180, 3412
BcuI ACTAGT 1 cut(s) 3280
BfaI CTAG 7 cut(s) 186, 762, 1176, 1604, 2582, 3281, 3413
BfmI CTRYAG 2 cut(s) 1081, 1860
BglII AGATCT 1 cut(s) 415
Bme1390I CCNGG 2 cut(s) 552, 2672
Bme18I GGWCC 2 cut(s) 1487, 3343
BmeT110I CYCGRG 1 cut(s) 2307
BmgBI CACGTC 3 cut(s) 1159, 2474, 2524
BmgT120I GGNCC 4 cut(s) 393, 1487, 3343, 3389
BmrFI CCNGG 2 cut(s) 552, 2672
BmrI ACTGGG 1 cut(s) 1875
BmsI GCATC 7 cut(s) 975, 1005, 2293, 2757, 3074, 3152, 3500
BmtI GCTAGC 1 cut(s) 1179
BmuI ACTGGG 1 cut(s) 1875
BpiI GAAGAC 4 cut(s) 117, 843, 986, 3560
BpmI CTGGAG 1 cut(s) 2556
Bpu10I CCTNAGC 1 cut(s) 3879
Bpu14I TTCGAA 2 cut(s) 18, 2826
BpuEI CTTGAG 5 cut(s) 683, 1601, 2573, 2639, 3101
BpuMI CCSGG 1 cut(s) 2672
BsaAI YACGTR 4 cut(s) 740, 2197, 2755, 3373
BsaHI GRCGYC 1 cut(s) 3421
BsaJI CCNNGG 5 cut(s) 330, 686, 1447, 1613, 2804
Bsc4I CCNNNNNNNGG 2 cut(s) 2044, 3656
Bse118I RCCGGY 2 cut(s) 723, 2770
Bse1I ACTGG 7 cut(s) 539, 1362, 1515, 1836, 1881, 2423, 3702
Bse3DI GCAATG 2 cut(s) 273, 991
BseBI CCWGG 1 cut(s) 552
BseDI CCNNGG 5 cut(s) 330, 686, 1447, 1613, 2804
BseGI GGATG 6 cut(s) 436, 807, 1137, 1777, 2308, 3315
BseLI CCNNNNNNNGG 2 cut(s) 2044, 3656
BseMI GCAATG 2 cut(s) 273, 991
BseMII CTCAG 6 cut(s) 185, 360, 479, 1926, 3378, 3870
BseNI ACTGG 7 cut(s) 539, 1362, 1515, 1836, 1881, 2423, 3702
BseRI GAGGAG 2 cut(s) 371, 1156
BseSI GKGCMC 2 cut(s) 3288, 3464
BseYI CCCAGC 1 cut(s) 1501
Bsh1285I CGRYCG 1 cut(s) 3155
BshFI GGCC 9 cut(s) 395, 706, 727, 754, 2426, 2619, 2803, 3390, 3877
BshNI GGYRCC 2 cut(s) 58, 2716
BsiEI CGRYCG 1 cut(s) 3155
BsiHKAI GWGCWC 2 cut(s) 860, 3464
BsiHKCI CYCGRG 1 cut(s) 2307
BsiSI CCGG 3 cut(s) 724, 2672, 2771
BslFI GGGAC 1 cut(s) 1664
BslI CCNNNNNNNGG 2 cut(s) 2044, 3656
BsmAI GTCTC 9 cut(s) 85, 169, 359, 688, 1201, 1293, 1684, 3180, 3412
BsmBI CGTCTC 2 cut(s) 1201, 3412
BsmFI GGGAC 1 cut(s) 1664
BsnI GGCC 9 cut(s) 395, 706, 727, 754, 2426, 2619, 2803, 3390, 3877
BsoBI CYCGRG 1 cut(s) 2307
Bsp119I TTCGAA 2 cut(s) 18, 2826
Bsp1286I GDGCHC 4 cut(s) 860, 2572, 3288, 3464
Bsp19I CCATGG 2 cut(s) 1447, 1613
BspANI GGCC 9 cut(s) 395, 706, 727, 754, 2426, 2619, 2803, 3390, 3877
BspCNI CTCAG 6 cut(s) 184, 361, 478, 1925, 3377, 3871
BspHI TCATGA 2 cut(s) 3430, 3694
BspMAI CTGCAG 1 cut(s) 1864
BspOI GCTAGC 1 cut(s) 1179
BspPI GGATC 7 cut(s) 139, 1718, 1783, 3206, 3532, 3699, 3750
BspQI GCTCTTC 1 cut(s) 1219
BspT104I TTCGAA 2 cut(s) 18, 2826
BspT107I GGYRCC 2 cut(s) 58, 2716
BsrBI CCGCTC 1 cut(s) 3323
BsrDI GCAATG 2 cut(s) 273, 991
BsrFI RCCGGY 2 cut(s) 723, 2770
BsrI ACTGG 7 cut(s) 539, 1362, 1515, 1836, 1881, 2423, 3702
BssAI RCCGGY 2 cut(s) 723, 2770
BssECI CCNNGG 5 cut(s) 330, 686, 1447, 1613, 2804
BssNAI GTATAC 2 cut(s) 3707, 3763
BssNI GRCGYC 1 cut(s) 3421
BssT1I CCWWGG 3 cut(s) 330, 1447, 1613
Bst1107I GTATAC 2 cut(s) 3707, 3763
Bst2UI CCWGG 1 cut(s) 552
Bst6I CTCTTC 6 cut(s) 20, 83, 686, 1219, 2757, 3748
BstACI GRCGYC 1 cut(s) 3421
BstBAI YACGTR 4 cut(s) 740, 2197, 2755, 3373
BstBI TTCGAA 2 cut(s) 18, 2826
BstC8I GCNNGC 4 cut(s) 1177, 2424, 2639, 3875
BstDEI CTNAG 8 cut(s) 171, 369, 465, 1912, 2561, 2939, 3364, 3879
BstDSI CCRYGG 3 cut(s) 686, 1447, 1613
BstF5I GGATG 6 cut(s) 436, 807, 1137, 1777, 2308, 3315
BstHHI GCGC 3 cut(s) 1260, 1737, 2643
BstMAI GTCTC 9 cut(s) 85, 169, 359, 688, 1201, 1293, 1684, 3180, 3412
BstMCI CGRYCG 1 cut(s) 3155
BstMWI GCNNNNNNNGC 5 cut(s) 513, 544, 1576, 1627, 3717
BstNI CCWGG 1 cut(s) 552
BstNSI RCATGY 3 cut(s) 668, 2206, 3561
BstSCI CCNGG 2 cut(s) 550, 2670
BstSFI CTRYAG 2 cut(s) 1081, 1860
BstSLI GKGCMC 2 cut(s) 3288, 3464
BstSNI TACGTA 1 cut(s) 740
BstV2I GAAGAC 4 cut(s) 117, 843, 986, 3560
BstX2I RGATCY 3 cut(s) 144, 415, 3524
BstYI RGATCY 3 cut(s) 144, 415, 3524
BstZ17I GTATAC 2 cut(s) 3707, 3763
BsuRI GGCC 9 cut(s) 395, 706, 727, 754, 2426, 2619, 2803, 3390, 3877
BtgI CCRYGG 3 cut(s) 686, 1447, 1613
BtgZI GCGATG 2 cut(s) 2476, 3833
BtrI CACGTC 3 cut(s) 1159, 2474, 2524
BtsCI GGATG 6 cut(s) 436, 807, 1137, 1777, 2308, 3315
BtsI GCAGTG 1 cut(s) 298
BtsIMutI CAGTG 4 cut(s) 124, 298, 990, 1522
Cac8I GCNNGC 4 cut(s) 1177, 2424, 2639, 3875
CciI TCATGA 2 cut(s) 3430, 3694
CfoI GCGC 3 cut(s) 1260, 1737, 2643
Cfr10I RCCGGY 2 cut(s) 723, 2770
Cfr13I GGNCC 4 cut(s) 393, 1487, 3343, 3389
CseI GACGC 1 cut(s) 1218
CsiI ACCWGGT 1 cut(s) 550
Csp6I GTAC 6 cut(s) 44, 616, 737, 2110, 2880, 3660
CspCI CAANNNNNGTGG 2 cut(s) 2455, 2490
CviQI GTAC 6 cut(s) 44, 616, 737, 2110, 2880, 3660
DdeI CTNAG 8 cut(s) 171, 369, 465, 1912, 2561, 2939, 3364, 3879
DraI TTTAAA 2 cut(s) 3220, 3400
DrdI GACNNNNNNGTC 1 cut(s) 119
DseDI GACNNNNNNGTC 1 cut(s) 119
EaeI YGGCCR 1 cut(s) 725
Eam1104I CTCTTC 6 cut(s) 20, 83, 686, 1219, 2757, 3748
EarI CTCTTC 6 cut(s) 20, 83, 686, 1219, 2757, 3748
EciI GGCGGA 1 cut(s) 2420
Eco105I TACGTA 1 cut(s) 740
Eco130I CCWWGG 3 cut(s) 330, 1447, 1613
Eco147I AGGCCT 2 cut(s) 754, 3877
Eco24I GRGCYC 1 cut(s) 2572
Eco32I GATATC 1 cut(s) 3271
Eco47I GGWCC 2 cut(s) 1487, 3343
Eco57I CTGAAG 4 cut(s) 129, 1425, 2478, 2757
Eco88I CYCGRG 1 cut(s) 2307
EcoO109I RGGNCCY 1 cut(s) 3389
EcoRII CCWGG 1 cut(s) 550
EcoRV GATATC 1 cut(s) 3271
EcoT14I CCWWGG 3 cut(s) 330, 1447, 1613
EcoT22I ATGCAT 1 cut(s) 1010
EcoT38I GRGCYC 1 cut(s) 2572
ErhI CCWWGG 3 cut(s) 330, 1447, 1613
Esp3I CGTCTC 2 cut(s) 1201, 3412
FalI AAGNNNNNCTT 2 cut(s) 1563, 1595
FaqI GGGAC 1 cut(s) 1664
FauI CCCGC 3 cut(s) 182, 971, 3328
FauNDI CATATG 2 cut(s) 2947, 3808
FbaI TGATCA 1 cut(s) 3499
FblI GTMKAC 6 cut(s) 1155, 2415, 3328, 3369, 3706, 3762
FokI GGATG 6 cut(s) 443, 814, 1144, 1784, 2315, 3302
FriOI GRGCYC 1 cut(s) 2572
FspAI RTGCGCAY 1 cut(s) 1736
FspBI CTAG 7 cut(s) 186, 762, 1176, 1604, 2582, 3281, 3413
FspI TGCGCA 2 cut(s) 1259, 1736
GlaI GCGC 3 cut(s) 1259, 1736, 2642
GsaI CCCAGC 1 cut(s) 1505
GsuI CTGGAG 1 cut(s) 2556
HaeIII GGCC 9 cut(s) 395, 706, 727, 754, 2426, 2619, 2803, 3390, 3877
HapII CCGG 3 cut(s) 724, 2672, 2771
HgaI GACGC 1 cut(s) 1218
HhaI GCGC 3 cut(s) 1260, 1737, 2643
Hin1I GRCGYC 1 cut(s) 3421
Hin6I GCGC 3 cut(s) 1258, 1735, 2641
HinP1I GCGC 3 cut(s) 1258, 1735, 2641
HincII GTYRAC 2 cut(s) 444, 1247
HindII GTYRAC 2 cut(s) 444, 1247
HindIII AAGCTT 3 cut(s) 1333, 1577, 1619
HpaII CCGG 3 cut(s) 724, 2672, 2771
Hpy99I CGWCG 2 cut(s) 278, 1054
HpyAV CCTTC 5 cut(s) 744, 1557, 2290, 2677, 3827
HpyF10VI GCNNNNNNNGC 5 cut(s) 513, 544, 1576, 1627, 3717
HpyF3I CTNAG 8 cut(s) 171, 369, 465, 1912, 2561, 2939, 3364, 3879
Hsp92I GRCGYC 1 cut(s) 3421
HspAI GCGC 3 cut(s) 1258, 1735, 2641
Ksp22I TGATCA 1 cut(s) 3499
LguI GCTCTTC 1 cut(s) 1219
LmnI GCTCC 4 cut(s) 898, 988, 1096, 2575
LweI GCATC 7 cut(s) 975, 1005, 2293, 2757, 3074, 3152, 3500
MabI ACCWGGT 1 cut(s) 550
MaeI CTAG 7 cut(s) 186, 762, 1176, 1604, 2582, 3281, 3413
MaeIII GTNAC 7 cut(s) 573, 1463, 1886, 2054, 3229, 3422, 3569
MbiI CCGCTC 1 cut(s) 3323
MfeI CAATTG 1 cut(s) 1625
MflI RGATCY 3 cut(s) 144, 415, 3524
MhlI GDGCHC 4 cut(s) 860, 2572, 3288, 3464
MlyI GAGTC 3 cut(s) 690, 2084, 3059
MmeI TCCRAC 4 cut(s) 34, 1688, 1777, 2029
Mph1103I ATGCAT 1 cut(s) 1010
MroXI GAANNNNTTC 4 cut(s) 132, 1759, 2742, 3897
MslI CAYNNNNRTG 1 cut(s) 3429
MspA1I CMGCKG 1 cut(s) 1986
MspI CCGG 3 cut(s) 724, 2672, 2771
MspR9I CCNGG 2 cut(s) 552, 2672
MunI CAATTG 1 cut(s) 1625
MvaI CCWGG 1 cut(s) 552
MwoI GCNNNNNNNGC 5 cut(s) 513, 544, 1576, 1627, 3717
NciI CCSGG 1 cut(s) 2672
NcoI CCATGG 2 cut(s) 1447, 1613
NdeI CATATG 2 cut(s) 2947, 3808
NheI GCTAGC 1 cut(s) 1175
NmeAIII GCCGAG 1 cut(s) 2785
NmuCI GTSAC 2 cut(s) 573, 3422
NsbI TGCGCA 2 cut(s) 1259, 1736
NsiI ATGCAT 1 cut(s) 1010
NspI RCATGY 3 cut(s) 668, 2206, 3561
NspV TTCGAA 2 cut(s) 18, 2826
PaeR7I CTCGAG 1 cut(s) 2307
PagI TCATGA 2 cut(s) 3430, 3694
PceI AGGCCT 2 cut(s) 754, 3877
PciI ACATGT 1 cut(s) 3557
PciSI GCTCTTC 1 cut(s) 1219
PdmI GAANNNNTTC 4 cut(s) 132, 1759, 2742, 3897
PflMI CCANNNNNTGG 1 cut(s) 2044
PleI GAGTC 3 cut(s) 690, 2083, 3058
PpsI GAGTC 3 cut(s) 690, 2083, 3058
Ppu21I YACGTR 4 cut(s) 740, 2197, 2755, 3373
PscI ACATGT 1 cut(s) 3557
PshBI ATTAAT 1 cut(s) 3675
Psp1406I AACGTT 1 cut(s) 181
Psp6I CCWGG 1 cut(s) 550
PspFI CCCAGC 1 cut(s) 1501
PspGI CCWGG 1 cut(s) 550
PspPI GGNCC 4 cut(s) 393, 1487, 3343, 3389
PstI CTGCAG 1 cut(s) 1864
PsuI RGATCY 3 cut(s) 144, 415, 3524
PvuII CAGCTG 1 cut(s) 1986
RsaI GTAC 6 cut(s) 45, 617, 738, 2111, 2881, 3661
RsaNI GTAC 6 cut(s) 44, 616, 737, 2110, 2880, 3660
RseI CAYNNNNRTG 1 cut(s) 3429
SapI GCTCTTC 1 cut(s) 1219
Sau96I GGNCC 4 cut(s) 393, 1487, 3343, 3389
SchI GAGTC 3 cut(s) 690, 2084, 3059
ScrFI CCNGG 2 cut(s) 552, 2672
SduI GDGCHC 4 cut(s) 860, 2572, 3288, 3464
SexAI ACCWGGT 1 cut(s) 550
SfaNI GCATC 7 cut(s) 975, 1005, 2293, 2757, 3074, 3152, 3500
SfcI CTRYAG 2 cut(s) 1081, 1860
Sfr274I CTCGAG 1 cut(s) 2307
SfuI TTCGAA 2 cut(s) 18, 2826
SinI GGWCC 2 cut(s) 1487, 3343
SlaI CTCGAG 1 cut(s) 2307
SmiMI CAYNNNNRTG 1 cut(s) 3429
SmlI CTYRAG 6 cut(s) 698, 1580, 2307, 2552, 2654, 3080
SmoI CTYRAG 6 cut(s) 698, 1580, 2307, 2552, 2654, 3080
SnaBI TACGTA 1 cut(s) 740
SpeI ACTAGT 1 cut(s) 3280
SseBI AGGCCT 2 cut(s) 754, 3877
SspI AATATT 4 cut(s) 622, 2594, 2605, 2785
SspMI CTAG 7 cut(s) 186, 762, 1176, 1604, 2582, 3281, 3413
StuI AGGCCT 2 cut(s) 754, 3877
StyD4I CCNGG 2 cut(s) 550, 2670
StyI CCWWGG 3 cut(s) 330, 1447, 1613
TaqI TCGA 9 cut(s) 18, 854, 1790, 2228, 2308, 2500, 2826, 3151, 3259
TatI WGTACW 2 cut(s) 43, 615
TauI GCSGC 1 cut(s) 398
TscAI CASTG 4 cut(s) 124, 298, 990, 1522
TseFI GTSAC 2 cut(s) 573, 3422
Tsp45I GTSAC 2 cut(s) 573, 3422
TspGWI ACGGA 2 cut(s) 703, 1219
TspRI CASTG 4 cut(s) 124, 298, 990, 1522
Van91I CCANNNNNTGG 1 cut(s) 2044
VneI GTGCAC 1 cut(s) 3460
VpaK11BI GGWCC 2 cut(s) 1487, 3343
VspI ATTAAT 1 cut(s) 3675
XapI RAATTY 9 cut(s) 217, 374, 920, 1024, 1673, 1757, 2173, 2248, 2510
XceI RCATGY 3 cut(s) 668, 2206, 3561
XcmI CCANNNNNNNNNTGG 2 cut(s) 328, 2045
XhoI CTCGAG 1 cut(s) 2307
XmiI GTMKAC 6 cut(s) 1155, 2415, 3328, 3369, 3706, 3762
XmnI GAANNNNTTC 4 cut(s) 132, 1759, 2742, 3897
XspI CTAG 7 cut(s) 186, 762, 1176, 1604, 2582, 3281, 3413
ZraI GACGTC 1 cut(s) 3422
Zsp2I ATGCAT 1 cut(s) 1010
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.