Rroxscaffold_6G00422590

FES

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0007319
Physical Location & Seq
Reverse (-)
43419553 .. 43430925
11373 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00422590.1

Sequence Viewer

Length: 3864 bp
ATGTCCTCTTTCGATTCAGAAATACCGGTAGTTTACGAGAGGCCGAAGAGACAGCACGTTGGGTCTGAAGACAACAGTGTCATGGAACGGGTTCAGGTTCAGATCCCAGATACTCCTGCAAAGAAAACTCAGAGACAACGTTCTAGCGGGATTTCTATGCTTACTGCCCCTACAAGTTCTTTAGAGGAAAACAAAGAGAACCCGATTGCAGAGGACTTGTCTTCAATGGACGACGATGTAGTGGATTGCAGTGGACTTATGGACACGATTTTGGATTCCATTGGTTCCTTGGATTTGAACAAGCCTGTGTGGGAGGAGTGGGAGACTGAGGAATTTGCTTATGGTTATAGGGCCGCATTATTGCAAACAGAAGATCTTGCAAGGGAGGATGAGGAAGTGTTGACAAATAATCTTCCAAGTTCTCAGTCATCTTCCAATACCGAAAATCCTGAAAATCACAGTTGCGGTTTTGGCAGCATAGAGGGTTCTTTCATGCCAGTTGTGCAACCAGGTTATGCTGCTGAATCCATGTCACAATATAATTTGAAGAATCAGGAATTAGTTGATGGCAAGTACAATATTGCTGATACAAATTATATTGGTAGCTTAAAAAGAAAACTACATGCTGCTACACTTCAAATGCCACGGAAGAGACTCAAGAGGCCGAAGAAAAAAGTTTACCGGCCAAAAGTGGTACGTAATTTAGGAAGGCCTATACCTAGAATGGAACCCAAGACTCCCACCAAAAAGAATGAAAGGATGAAGCTCAAAACACCAAAAATGAAGAAAAATGTGTCATTGAAGAAGTCTTCGGGCACAATCAACCTCAACCATGACCAAGTTCCTTTAGATTTGGAGCCTATTGTATCAGAGGGAGAAGTTTCAAGCATTGACATAGAAAAGCACTCTAACGATGGAACCCGCATCATATCAGAGAAGACAGTGGAGCATTGCATCACAAAATGCATTGATTTTGATATAGGATTAAATTTTTTGACGAAGAAAAAGAGGACGACGATGGGAAAGAAATGGGAAGTGAAAGACTATAGTAGAATAATGGAACCGAATCTTCTGAAAATAAAAAAGAAAAAAAGGATGGGCAGGAGGAGATGGGAAGTAGACGTGTGGAAAAAAATAGCTAGCAAGTTTCAGACAAACTATCTTAAACGGAGACGCAATAAAAAGAGAAGAGCTGCCCCCGAAATCAGTCAACAGGAAGGGCGCAACATCTTTAACACCGAAAAGCAGTTGTCTCCATGCTCCATCACCGAATCAGGAGTATTGAACATTGAAGAAAGCTTAACAGGAATGGTGTGCTTACTGGAAAGTGATAGTTTTGAGTTGTTCTTCGAATCAACAGATATACTTGAAGATAATATCAATGTTGTTGTGCAAAAACATCAACTTAACCATGGGATACTTGTTTGTTACCAAGAAAATGAGAAACATGGTCCTCATGTTGAGCTGGGTATCGTAGCCAGTGCAAATGGTGAGCTTGGTATGGTAGCCAAGACAAAGGGAAAGGGAAGGAGGCAAGAAAAGCTTGAGAAATTAGAACATGGTGAACTAGCTCACCCATGGAAGCTTCAATTGCAAAATGAAGTTAAGGAGGGGGACAAACATGACCCGAATTCTGAATTTTGGGATAAGGAGAGACAATTCTTTCAGGGTCGGATTGACTTATTTTTAACTCGTATGCGCATAGTTCAAGGAAATAGAAAATTTTCAGAATGGGATGGATCAGTTATGGATTCGATAGTCGGAGTTTTTTTAACACAGAACGTATCAGATCATCTATCCAGTTCTGCCTTTATATCACTCGCTGCAACTTTCCCATATCAGCCCAGTTGTAACCAGGAATTAACTTATGCAAACTCAGAGCCAAGTTGTGAGCAATCAAGAATTGTTAAAATTGAGGAATTAGTAGAGGGTGGAATCTCAAAGTCCAGCTGTGAATCACAATCAAATGACCAAACATATTGTGAAGATATAAATAGTGGCAGCCATAAATTGGTTGGAAGTAACATGGTATTTGATAAAGAGTCGTTCACACAAGAAGCCACAACTACAATGAAGTACGAAAATCAGAAGTCAAGTGATTGCGAAATGGTATTAGCCAAACAAGTTGAGTTGCAGCAAAATTTTGAGGGTCAAGATTATTACGTGGACATGCCATCTGATAAGCCTGACTGCTCGATTGATATTATTACTGAAAATTCGCTTTCTAACTTCAATGAGGAAAAGCAAAGAAGAAAGAAGTTGAAGGTGGATGCTCGAGAAATAAATAATGAGGGTGCTATGGTGAATGGAAAACCTGATTGGGATTATTATAGGAGAGACATTGAAAAGGAAAGAAAGATCAGGCGCATGGCGGATTCGGTAGACTGGCAGGCCGTTAGACAAGCCGATGTTGGTGATATTGCTGAAGCCATCAAAGGACGTGGACAACATCGTGTCATCGCAGATCGAATAAAGAAATTTCTTAATGACGTGAATCAAGACCATAAAGAAATAAACCTTGAGTGGCTAAGATGGGCTCCAGACAAACTAGCAAGGAAATATTTGACTAATATCGAGGGAATTGGCCTGAAAAGTGTAGAGTGCGTGCGCCTTTTAGCACTCAAGCAGATAGCCTTCCCGGTGGACGTTAATGTTGGCAGGATAGCCATTCGTTTAGGGTGGGTGCCTATGCAACCATTACCTGAAGATGTTCAGATACACGTATTAGAAGAGATGCCGGTTATGGACAATATTCAGAAGTATCTTTGGCCTCGGTTAATGAAATTGACGTTCGAAGAACTGTATGAGCTACACTATCAAATGATAACCTTTGGAAAGGTTTATTGTACCAAACGTAAACCTAATTGTCATTCATGTCCAATGAAGAACGAGTGCAAGTATTATCTTAGTGCATATGCAAGTTCAAAATATGCTGCTTTACCGAAGTCAATGAAGAAGCAAGGACAAAAGAAAACTGATGTGGTTATTTGCAATAATCATCTTTCTTCTGGGTACGAGCCAGTAAATGCAGCATCACAATCATATCTTGAGGACACTACTAAATTAGAATGTCAAATAAAAGATTGTGAACCGATAATTGAAGAACCAGCATCACCTCGACCGTCATTACCGGAACCAGAATATGTGGAAAGAGACATTGAAGATTTATTCCTTGATGATCCTTTTAAAGATTTCGTAACAAGAAGTCCATATATGAATACATTTCGAGGAAGTGATATCTCAACACTAGTTCCCTTTATGACCCCAGAAGACGCTGCTTTCATCCCCGCTCGTAGACTAAAAGAAGTGGACCGTTTGAGGACAAAACACTCGGTCTACGTGATTAAAGATCAAGGCCCTTTATTTAAAGAGCTACAACTAGAGAGACGTCACCCTCATGATAATAGTCCTTACCTTTTTGCCTTGTGCACATCAGATCAAATGGCAAAAGTATCTCAATCACCTCAAAACAGATGCAGTTGCAAGGGATCTAATGATTCATCATGTGATGGTGAGAAGACATGTTTTTCTTGTAACAGTCTTTCAACACAAGCTAATACTGTTCAAGGAACAATTTTGGTTTTTGCGGATCATGAATCCAGTATACGCCCCATCAACGTTCATAGAGATTTGTTATGGGATCGGACGAGAAGAGATGTATACTTTGGAACATCAACAAGTGCAATTCTTAAAGGCTTATCAACATATGACGTTGCGATGTGTTTTAAGGAAGGTTATATTTGCGTTAGAGCATTTAATCGGGAAACACGCAGGCCTGAGCCTCTACTAGAAAGGTTTCACATTTCAACCGTCAAAACCGCAAAGAAAGGAAAGAAAAAAGCGGAACAGAAAGAAGACGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Perm-CXXC RRM_DME

Protein Analysis

1287

Amino Acids

147.46

Weight (kDa)

7.12

Isoelectric Point (pI)

52.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_DME PF15628 1194 - 1268 8.8e-26 RRM in Demeter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 77
AatII GACGTC 1 cut(s) 3391
Acc16I TGCGCA 1 cut(s) 1700
AccB1I GGYRCC 1 cut(s) 2683
AccB7I CCANNNNNTGG 1 cut(s) 2011
AccBSI CCGCTC 1 cut(s) 3290
AccI GTMKAC 6 cut(s) 1119, 2382, 3295, 3336, 3604, 3660
AciI CCGC 9 cut(s) 147, 354, 465, 922, 2372, 3288, 3587, 3819, 3842
AclI AACGTT 2 cut(s) 139, 3618
AclWI GGATC 6 cut(s) 97, 1747, 3173, 3496, 3597, 3648
AcoI YGGCCR 1 cut(s) 683
AcsI RAATTY 8 cut(s) 332, 988, 1630, 1637, 1721, 2140, 2215, 2477
AcuI CTGAAG 3 cut(s) 87, 2445, 2724
AcyI GRCGYC 1 cut(s) 3388
AfaI GTAC 5 cut(s) 575, 696, 2078, 2848, 3014
AfiI CCNNNNNNNGG 1 cut(s) 2011
AflIII ACRYGT 3 cut(s) 1122, 2719, 3521
AgeI ACCGGT 1 cut(s) 25
AhlI ACTAGT 1 cut(s) 3247
AjiI CACGTC 3 cut(s) 1123, 2441, 2491
AjnI CCWGG 2 cut(s) 508, 1854
AjuI GAANNNNNNNTTGG 2 cut(s) 2302, 2334
Alw21I GWGCWC 1 cut(s) 3431
Alw44I GTGCAC 1 cut(s) 3427
AlwI GGATC 6 cut(s) 97, 1747, 3173, 3496, 3597, 3648
Ama87I CYCGRG 1 cut(s) 2274
ApaLI GTGCAC 1 cut(s) 3427
ApoI RAATTY 8 cut(s) 332, 988, 1630, 1637, 1721, 2140, 2215, 2477
AsiGI ACCGGT 1 cut(s) 25
Asp700I GAANNNNTTC 4 cut(s) 90, 1723, 2709, 3795
AspLEI GCGC 4 cut(s) 1224, 1701, 2367, 2610
AspS9I GGNCC 4 cut(s) 351, 1451, 3310, 3356
AsuC2I CCSGG 1 cut(s) 2639
AsuII TTCGAA 2 cut(s) 1350, 2793
AsuNHI GCTAGC 1 cut(s) 1139
AvaI CYCGRG 1 cut(s) 2274
AvaII GGWCC 2 cut(s) 1451, 3310
BaeGI GKGCMC 2 cut(s) 818, 3431
BanI GGYRCC 1 cut(s) 2683
BanII GRGCYC 1 cut(s) 2539
BbsI GAAGAC 6 cut(s) 75, 213, 801, 944, 3276, 3524
Bbv12I GWGCWC 1 cut(s) 3431
BceAI ACGGC 1 cut(s) 2378
BciT130I CCWGG 2 cut(s) 510, 1856
BciVI GTATCC 1 cut(s) 1410
BcnI CCSGG 1 cut(s) 2639
BcuI ACTAGT 1 cut(s) 3247
BfaI CTAG 8 cut(s) 144, 720, 1140, 1568, 2549, 3248, 3380, 3788
BfmI CTRYAG 1 cut(s) 1045
BfuI GTATCC 1 cut(s) 1410
BglII AGATCT 1 cut(s) 373
Bme1390I CCNGG 3 cut(s) 510, 1856, 2639
Bme18I GGWCC 2 cut(s) 1451, 3310
BmeT110I CYCGRG 1 cut(s) 2274
BmgBI CACGTC 3 cut(s) 1123, 2441, 2491
BmgT120I GGNCC 4 cut(s) 351, 1451, 3310, 3356
BmiI GGNNCC 8 cut(s) 286, 729, 858, 919, 1062, 2538, 2685, 3135
BmrFI CCNGG 3 cut(s) 510, 1856, 2639
BmrI ACTGGG 1 cut(s) 1839
BmsI GCATC 7 cut(s) 933, 963, 2260, 2724, 3041, 3119, 3464
BmtI GCTAGC 1 cut(s) 1143
BmuI ACTGGG 1 cut(s) 1839
BpiI GAAGAC 6 cut(s) 75, 213, 801, 944, 3276, 3524
BpmI CTGGAG 1 cut(s) 2523
Bpu10I CCTNAGC 1 cut(s) 3777
Bpu14I TTCGAA 2 cut(s) 1350, 2793
BpuEI CTTGAG 5 cut(s) 641, 1565, 2540, 2606, 3068
BpuMI CCSGG 1 cut(s) 2639
BsaAI YACGTR 4 cut(s) 698, 2164, 2722, 3340
BsaHI GRCGYC 1 cut(s) 3388
BsaJI CCNNGG 5 cut(s) 288, 644, 1411, 1577, 2771
BsaWI WCCGGW 2 cut(s) 25, 3130
Bsc4I CCNNNNNNNGG 1 cut(s) 2011
Bse118I RCCGGY 3 cut(s) 25, 681, 2737
Bse1I ACTGG 8 cut(s) 497, 1326, 1479, 1800, 1845, 2390, 3020, 3600
Bse3DI GCAATG 1 cut(s) 949
BseBI CCWGG 2 cut(s) 510, 1856
BseDI CCNNGG 5 cut(s) 288, 644, 1411, 1577, 2771
BseGI GGATG 6 cut(s) 394, 765, 1101, 1741, 2275, 3282
BseLI CCNNNNNNNGG 1 cut(s) 2011
BseMI GCAATG 1 cut(s) 949
BseMII CTCAG 5 cut(s) 143, 318, 437, 1890, 3768
BseNI ACTGG 8 cut(s) 497, 1326, 1479, 1800, 1845, 2390, 3020, 3600
BseRI GAGGAG 2 cut(s) 329, 1120
BseSI GKGCMC 2 cut(s) 818, 3431
BseYI CCCAGC 1 cut(s) 1465
Bsh1285I CGRYCG 1 cut(s) 3122
BshNI GGYRCC 1 cut(s) 2683
BshTI ACCGGT 1 cut(s) 25
BsiEI CGRYCG 1 cut(s) 3122
BsiHKAI GWGCWC 1 cut(s) 3431
BsiHKCI CYCGRG 1 cut(s) 2274
BsiSI CCGG 5 cut(s) 26, 682, 2639, 2738, 3131
BslFI GGGAC 1 cut(s) 1628
BslI CCNNNNNNNGG 1 cut(s) 2011
BsmBI CGTCTC 2 cut(s) 1165, 3379
BsmFI GGGAC 1 cut(s) 1628
BsoBI CYCGRG 1 cut(s) 2274
Bsp119I TTCGAA 2 cut(s) 1350, 2793
Bsp1286I GDGCHC 3 cut(s) 818, 2539, 3431
Bsp19I CCATGG 2 cut(s) 1411, 1577
BspACI CCGC 9 cut(s) 147, 354, 465, 922, 2372, 3288, 3587, 3819, 3842
BspCNI CTCAG 5 cut(s) 142, 319, 436, 1889, 3769
BspHI TCATGA 2 cut(s) 3397, 3592
BspLI GGNNCC 8 cut(s) 286, 729, 858, 919, 1062, 2538, 2685, 3135
BspOI GCTAGC 1 cut(s) 1143
BspPI GGATC 6 cut(s) 97, 1747, 3173, 3496, 3597, 3648
BspQI GCTCTTC 1 cut(s) 1183
BspT104I TTCGAA 2 cut(s) 1350, 2793
BspT107I GGYRCC 1 cut(s) 2683
BsrBI CCGCTC 1 cut(s) 3290
BsrDI GCAATG 1 cut(s) 949
BsrFI RCCGGY 3 cut(s) 25, 681, 2737
BsrI ACTGG 8 cut(s) 497, 1326, 1479, 1800, 1845, 2390, 3020, 3600
BssAI RCCGGY 3 cut(s) 25, 681, 2737
BssECI CCNNGG 5 cut(s) 288, 644, 1411, 1577, 2771
BssNAI GTATAC 2 cut(s) 3605, 3661
BssNI GRCGYC 1 cut(s) 3388
BssT1I CCWWGG 3 cut(s) 288, 1411, 1577
Bst1107I GTATAC 2 cut(s) 3605, 3661
Bst2UI CCWGG 2 cut(s) 510, 1856
Bst4CI ACNGT 9 cut(s) 77, 461, 943, 2802, 3123, 3314, 3539, 3562, 3811
Bst6I CTCTTC 5 cut(s) 41, 644, 1183, 2724, 3646
BstACI GRCGYC 1 cut(s) 3388
BstBAI YACGTR 4 cut(s) 698, 2164, 2722, 3340
BstBI TTCGAA 2 cut(s) 1350, 2793
BstC8I GCNNGC 4 cut(s) 1141, 2391, 2606, 3773
BstDEI CTNAG 7 cut(s) 129, 327, 423, 1876, 2528, 2906, 3777
BstDSI CCRYGG 3 cut(s) 644, 1411, 1577
BstF5I GGATG 6 cut(s) 394, 765, 1101, 1741, 2275, 3282
BstHHI GCGC 4 cut(s) 1224, 1701, 2367, 2610
BstMCI CGRYCG 1 cut(s) 3122
BstMWI GCNNNNNNNGC 4 cut(s) 471, 502, 1540, 1591
BstNI CCWGG 2 cut(s) 510, 1856
BstNSI RCATGY 3 cut(s) 626, 2173, 3525
BstSCI CCNGG 3 cut(s) 508, 1854, 2637
BstSFI CTRYAG 1 cut(s) 1045
BstSLI GKGCMC 2 cut(s) 818, 3431
BstSNI TACGTA 1 cut(s) 698
BstV2I GAAGAC 6 cut(s) 75, 213, 801, 944, 3276, 3524
BstX2I RGATCY 3 cut(s) 102, 373, 3488
BstYI RGATCY 3 cut(s) 102, 373, 3488
BstZ17I GTATAC 2 cut(s) 3605, 3661
BsuI GTATCC 1 cut(s) 1410
BtgI CCRYGG 3 cut(s) 644, 1411, 1577
BtgZI GCGATG 2 cut(s) 2443, 3731
BtrI CACGTC 3 cut(s) 1123, 2441, 2491
BtsCI GGATG 6 cut(s) 394, 765, 1101, 1741, 2275, 3282
BtsI GCAGTG 1 cut(s) 256
BtsIMutI CAGTG 4 cut(s) 82, 256, 948, 1486
Cac8I GCNNGC 4 cut(s) 1141, 2391, 2606, 3773
CciI TCATGA 2 cut(s) 3397, 3592
CfoI GCGC 4 cut(s) 1224, 1701, 2367, 2610
Cfr10I RCCGGY 3 cut(s) 25, 681, 2737
Cfr13I GGNCC 4 cut(s) 351, 1451, 3310, 3356
CseI GACGC 2 cut(s) 1182, 3281
CsiI ACCWGGT 1 cut(s) 508
Csp6I GTAC 5 cut(s) 574, 695, 2077, 2847, 3013
CspAI ACCGGT 1 cut(s) 25
CspCI CAANNNNNGTGG 2 cut(s) 2422, 2457
CviQI GTAC 5 cut(s) 574, 695, 2077, 2847, 3013
DdeI CTNAG 7 cut(s) 129, 327, 423, 1876, 2528, 2906, 3777
DraI TTTAAA 2 cut(s) 3187, 3367
DrdI GACNNNNNNGTC 1 cut(s) 77
DseDI GACNNNNNNGTC 1 cut(s) 77
EaeI YGGCCR 1 cut(s) 683
Eam1104I CTCTTC 5 cut(s) 41, 644, 1183, 2724, 3646
EarI CTCTTC 5 cut(s) 41, 644, 1183, 2724, 3646
EciI GGCGGA 1 cut(s) 2387
Eco105I TACGTA 1 cut(s) 698
Eco130I CCWWGG 3 cut(s) 288, 1411, 1577
Eco147I AGGCCT 2 cut(s) 712, 3775
Eco24I GRGCYC 1 cut(s) 2539
Eco32I GATATC 1 cut(s) 3238
Eco47I GGWCC 2 cut(s) 1451, 3310
Eco57I CTGAAG 3 cut(s) 87, 2445, 2724
Eco88I CYCGRG 1 cut(s) 2274
EcoO109I RGGNCCY 1 cut(s) 3356
EcoRI GAATTC 1 cut(s) 1630
EcoRII CCWGG 2 cut(s) 508, 1854
EcoRV GATATC 1 cut(s) 3238
EcoT14I CCWWGG 3 cut(s) 288, 1411, 1577
EcoT22I ATGCAT 1 cut(s) 968
EcoT38I GRGCYC 1 cut(s) 2539
ErhI CCWWGG 3 cut(s) 288, 1411, 1577
Esp3I CGTCTC 2 cut(s) 1165, 3379
FalI AAGNNNNNCTT 2 cut(s) 1527, 1559
FaqI GGGAC 1 cut(s) 1628
FauI CCCGC 3 cut(s) 140, 929, 3295
FauNDI CATATG 2 cut(s) 2914, 3706
FblI GTMKAC 6 cut(s) 1119, 2382, 3295, 3336, 3604, 3660
FokI GGATG 6 cut(s) 401, 772, 1108, 1748, 2282, 3269
FriOI GRGCYC 1 cut(s) 2539
FspAI RTGCGCAY 1 cut(s) 1700
FspBI CTAG 8 cut(s) 144, 720, 1140, 1568, 2549, 3248, 3380, 3788
FspI TGCGCA 1 cut(s) 1700
GlaI GCGC 4 cut(s) 1223, 1700, 2366, 2609
GsaI CCCAGC 1 cut(s) 1469
GsuI CTGGAG 1 cut(s) 2523
HapII CCGG 5 cut(s) 26, 682, 2639, 2738, 3131
HgaI GACGC 2 cut(s) 1182, 3281
HhaI GCGC 4 cut(s) 1224, 1701, 2367, 2610
Hin1I GRCGYC 1 cut(s) 3388
Hin6I GCGC 4 cut(s) 1222, 1699, 2365, 2608
HinP1I GCGC 4 cut(s) 1222, 1699, 2365, 2608
HincII GTYRAC 2 cut(s) 402, 1211
HindII GTYRAC 2 cut(s) 402, 1211
HindIII AAGCTT 3 cut(s) 1297, 1541, 1583
HpaII CCGG 5 cut(s) 26, 682, 2639, 2738, 3131
Hpy99I CGWCG 2 cut(s) 236, 1018
HpyAV CCTTC 6 cut(s) 702, 1211, 1521, 2257, 2644, 3725
HpyCH4III ACNGT 9 cut(s) 77, 461, 943, 2802, 3123, 3314, 3539, 3562, 3811
HpyF10VI GCNNNNNNNGC 4 cut(s) 471, 502, 1540, 1591
HpyF3I CTNAG 7 cut(s) 129, 327, 423, 1876, 2528, 2906, 3777
Hsp92I GRCGYC 1 cut(s) 3388
HspAI GCGC 4 cut(s) 1222, 1699, 2365, 2608
LguI GCTCTTC 1 cut(s) 1183
LmnI GCTCC 4 cut(s) 856, 946, 1265, 2542
LweI GCATC 7 cut(s) 933, 963, 2260, 2724, 3041, 3119, 3464
MabI ACCWGGT 1 cut(s) 508
MaeI CTAG 8 cut(s) 144, 720, 1140, 1568, 2549, 3248, 3380, 3788
MaeIII GTNAC 7 cut(s) 531, 1427, 1850, 2021, 3196, 3389, 3533
MbiI CCGCTC 1 cut(s) 3290
MfeI CAATTG 1 cut(s) 1589
MflI RGATCY 3 cut(s) 102, 373, 3488
MhlI GDGCHC 3 cut(s) 818, 2539, 3431
MlyI GAGTC 3 cut(s) 648, 730, 2051
MmeI TCCRAC 3 cut(s) 1652, 1741, 1996
Mph1103I ATGCAT 1 cut(s) 968
MroXI GAANNNNTTC 4 cut(s) 90, 1723, 2709, 3795
MslI CAYNNNNRTG 1 cut(s) 3396
MspA1I CMGCKG 1 cut(s) 1950
MspI CCGG 5 cut(s) 26, 682, 2639, 2738, 3131
MspR9I CCNGG 3 cut(s) 510, 1856, 2639
MunI CAATTG 1 cut(s) 1589
MvaI CCWGG 2 cut(s) 510, 1856
MwoI GCNNNNNNNGC 4 cut(s) 471, 502, 1540, 1591
NciI CCSGG 1 cut(s) 2639
NcoI CCATGG 2 cut(s) 1411, 1577
NdeI CATATG 2 cut(s) 2914, 3706
NheI GCTAGC 1 cut(s) 1139
NlaIV GGNNCC 8 cut(s) 286, 729, 858, 919, 1062, 2538, 2685, 3135
NmuCI GTSAC 2 cut(s) 531, 3389
NsbI TGCGCA 1 cut(s) 1700
NsiI ATGCAT 1 cut(s) 968
NspI RCATGY 3 cut(s) 626, 2173, 3525
NspV TTCGAA 2 cut(s) 1350, 2793
PaeR7I CTCGAG 1 cut(s) 2274
PagI TCATGA 2 cut(s) 3397, 3592
PceI AGGCCT 2 cut(s) 712, 3775
PciI ACATGT 1 cut(s) 3521
PciSI GCTCTTC 1 cut(s) 1183
PdmI GAANNNNTTC 4 cut(s) 90, 1723, 2709, 3795
PflMI CCANNNNNTGG 1 cut(s) 2011
PinAI ACCGGT 1 cut(s) 25
PleI GAGTC 3 cut(s) 648, 730, 2050
PpsI GAGTC 3 cut(s) 648, 730, 2050
Ppu21I YACGTR 4 cut(s) 698, 2164, 2722, 3340
PscI ACATGT 1 cut(s) 3521
Psp1406I AACGTT 2 cut(s) 139, 3618
Psp6I CCWGG 2 cut(s) 508, 1854
PspFI CCCAGC 1 cut(s) 1465
PspGI CCWGG 2 cut(s) 508, 1854
PspN4I GGNNCC 8 cut(s) 286, 729, 858, 919, 1062, 2538, 2685, 3135
PspPI GGNCC 4 cut(s) 351, 1451, 3310, 3356
PsuI RGATCY 3 cut(s) 102, 373, 3488
PvuII CAGCTG 1 cut(s) 1950
RsaI GTAC 5 cut(s) 575, 696, 2078, 2848, 3014
RsaNI GTAC 5 cut(s) 574, 695, 2077, 2847, 3013
RseI CAYNNNNRTG 1 cut(s) 3396
SapI GCTCTTC 1 cut(s) 1183
Sau96I GGNCC 4 cut(s) 351, 1451, 3310, 3356
SchI GAGTC 3 cut(s) 648, 730, 2051
ScrFI CCNGG 3 cut(s) 510, 1856, 2639
SduI GDGCHC 3 cut(s) 818, 2539, 3431
SexAI ACCWGGT 1 cut(s) 508
SfaNI GCATC 7 cut(s) 933, 963, 2260, 2724, 3041, 3119, 3464
SfcI CTRYAG 1 cut(s) 1045
Sfr274I CTCGAG 1 cut(s) 2274
SfuI TTCGAA 2 cut(s) 1350, 2793
SinI GGWCC 2 cut(s) 1451, 3310
SlaI CTCGAG 1 cut(s) 2274
SmiMI CAYNNNNRTG 1 cut(s) 3396
SmlI CTYRAG 6 cut(s) 656, 1544, 2274, 2519, 2621, 3047
SmoI CTYRAG 6 cut(s) 656, 1544, 2274, 2519, 2621, 3047
SnaBI TACGTA 1 cut(s) 698
SpeI ACTAGT 1 cut(s) 3247
SseBI AGGCCT 2 cut(s) 712, 3775
SsiI CCGC 9 cut(s) 147, 354, 465, 922, 2372, 3288, 3587, 3819, 3842
SspI AATATT 3 cut(s) 580, 2561, 2752
SspMI CTAG 8 cut(s) 144, 720, 1140, 1568, 2549, 3248, 3380, 3788
StuI AGGCCT 2 cut(s) 712, 3775
StyD4I CCNGG 3 cut(s) 508, 1854, 2637
StyI CCWWGG 3 cut(s) 288, 1411, 1577
TaaI ACNGT 9 cut(s) 77, 461, 943, 2802, 3123, 3314, 3539, 3562, 3811
TaqII GACCGA 1 cut(s) 3322
TatI WGTACW 1 cut(s) 573
TauI GCSGC 1 cut(s) 356
TscAI CASTG 4 cut(s) 82, 256, 948, 1486
TseFI GTSAC 2 cut(s) 531, 3389
Tsp45I GTSAC 2 cut(s) 531, 3389
TspGWI ACGGA 2 cut(s) 661, 1183
TspRI CASTG 4 cut(s) 82, 256, 948, 1486
Van91I CCANNNNNTGG 1 cut(s) 2011
VneI GTGCAC 1 cut(s) 3427
VpaK11BI GGWCC 2 cut(s) 1451, 3310
XapI RAATTY 8 cut(s) 332, 988, 1630, 1637, 1721, 2140, 2215, 2477
XceI RCATGY 3 cut(s) 626, 2173, 3525
XcmI CCANNNNNNNNNTGG 2 cut(s) 286, 2012
XhoI CTCGAG 1 cut(s) 2274
XmiI GTMKAC 6 cut(s) 1119, 2382, 3295, 3336, 3604, 3660
XmnI GAANNNNTTC 4 cut(s) 90, 1723, 2709, 3795
XspI CTAG 8 cut(s) 144, 720, 1140, 1568, 2549, 3248, 3380, 3788
ZraI GACGTC 1 cut(s) 3389
Zsp2I ATGCAT 1 cut(s) 968
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.